DOCSLIB.ORG

Cytogenetics of Edalorhina Perezi (Anura, Leptodactylidae)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Cytogenetics of Edalorhina Perezi (Anura, Leptodactylidae) C 2000 The Japan Mendel Society Cytologia 65: 359-363, 2000 Cytogenetics of Edalorhina perezi (Anura, Leptodactylidae) L. B. Lourengol, A. J. Cardoso" and S. M. Recco-Pimentel1 1 Departamento de Biologia Celular and 2 Departamento de Zoologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP) 13083-970, Campinas, SP, Brasil Accepted June 25, 2000 Summary Specimens of Edalorhina perezi Jimenez de la Espada from the Brazilian Amazon were studied cytogenetically using Giemsa staining, silver staining and C-banding. The diploid comple- ment of 2n=22 agreed with the chromosome number previously described for Edalorhina sp. Mor- phologically, the E. perezi karyotype resembled those of Physalaemus and Pleurodema species, but NOR localization and C-banding were unable to detect accurate homologies between E. perezi kary- otype and those of the Physalaemus and Pleurodema species studied to date. Key words Edalorhina, Leptodactylidae, cytogenetics. Currently, the genus Edalorhina (Leptodactylidae, Leptodactylinae) encompasses 2 species, E. perezi Jimenez de la Espada and E. nasuta Boulenger, as originally proposed by Dunn (1949). E. perezi is widespread in lowland tropical rainforest of the Amazon Basin in Ecuador, Peru, Colom- bia and Brazil, whereas E. nasuta is known only from Peru (Duellman and Morales 1990). Based on morphological and ecological characteristics, Duellman and Morales (1990) described geo- graphic variation in E. perezi and suggested the need for more studies on this species. The phylogenetic relationships of Edalorhina are still not clear. Dunn (1949) considered this genus closely related to Pleurodema, while Lynch (1971) placed Edalorhina between Lithodytes and Physalaemus. The proximity between Edalorhina and Pseudopaludicola was suggested by Heyer (1975), and Bogart (1976) proposed the inclusion of Edalorhina in the genus Physalaemus. In Heyer's study the chromosome number of Edalorhina sp. (Bogart 1973) was considered to- gether with ecological and morphological characters. Bogart (1976) also considered karyological information in his analysis, although no description of the Edalorhina karyotype, other than its chromosome number, has been reported. In this report we describe a more complete cytogenetic analysis of E. perezi in order to contribute for the assembly of chromosomal data for the study of the subfamily Leptodactylinae. Materials and methods Eight specimens (2 females, 5 males and 1 tadpole) of E. perezi from the Alto Jurua Reserve, in Acre State, Brazil, collected in February 1994 and January 1996 were examined. All adult ani- mals were deposited in the "Ada) Jose Cardoso" Zoology Museum at the State University of Camp- inas (ZUEC). The chromosomal preparations were obtained from suspensions of intestinal and testis cells from animals treated with colchicine for at least 4 h, as described by Schmid (1978) and Schmid et al. (1979). Conventional staining with Giemsa solution, silver staining (Howell and Black 1980) and C-banding (King 1980) were used to study the chromosomes, which were classified according to Green and Sessions (1991). * Dr . Adao Jose Cardoso died before this work was finished. 360 L. B. Lourenco, A. J. Cardoso and S. M. Recco-Pimentel Cytologia 65 Results All specimens had a full diploid chromosome complement of 2n=22 consisting of 6 pairs of metacentric chromosomes (pairs 1, 5, 6, 9-11), 4 pairs of submetacentric chromosomes and 1 pair of subacrocentric chromosomes (pair 3) (Fig. 1, Table 1). No heteromorphic sex chromosomes were detected. The nucleolus organizer region (NOR) was located on the long arm of chromosome 8 (Fig. 1C,D), in a region that appeared as a secondary constriction in Giemsa stained metaphases A B C D E F Fig. 1. Karyotype of Edalorhina perezi after Giemsa staining (A, B), silver staining (C, D) and C- banding (E, F). The arrows in A-D indicate the NOR. In F, the small arrows indicate faint C-bands and the large arrow points a conspicuous band of chromosome pair 8. Bar= 5 ƒÊm. 2000 Cytogenetics of Edalorhina perezi 361 Table 1. Morphometric analysis of the Edalorhina perezi karyotype. The values are the meant S.D. of measurements for 29 metaphases from 8 specimens M = metacentric, SM = submetacentric, SA= subacrocentric. (Fig. 1A, B). Neither intra- nor interindividual variability was observed in the occurrence of NOR based on silver staining. The C-banding pattern of the E. perezi karyo- type was determined in 2 specimens. The cen- tromeric region of all the chromosomes and an interstitial segment adjacent to the NOR stained strongly by the C-banding technique. Two other regions, one located interstitially on the short arm of chromosome 1 and the other in a pericen- tromeric segment of the long arm of chromo- some 2, stained faintly in C-banded metaphases Fig. 2. Idiogram of the Edalorhina perezi karyotype based on the morphometric data of Table 1. Solid blocks: (Fig. 1E, F). dark C-bands. Gray blocks: faint C-bands. Open area: sec- ondary constriction. Circles: NOR. Discussion The chromosome number of 2n=22 observed in this population of E. perezi from the Brazilian Amazon is the same as that described by Bogart (1973) for Edalorhina sp. Comparing Edalorhina with the genera considered to be closely related, we can observe that only Lithodytes lineatus (2n=18) (Bogart 1970), the unique species of this genus to be studied karyologically, Pseudopalu- dicola ameghini (2n=20) (Becak 1968), and some cytotypes of Pseudopaludicola falcipes (2n=16, 18, 20) (Batistic et al. 1969) have a diploid chromosome number different from 22. The karyotypes of all Physalaemus and Pleurodema species described to date (Kuramoto 1990, Lourenco et al. 1999) and one cytotype of Pseudopaludicola falcipes (Saez and Brum 1960) have diploid comple- ments of 22 chromosomes, as observed for E. perezi. In addition to their chromosome number, the chromosomal morphology of the karyotypes of E. perezi, Pleurodema species (Barrio and Rinaldi de Chieri 1970, Duellman and Veloso 1977, Schmid et al. 1993) and most Physalaemus species (Becak et al. 1970, De Lucca et al. 1974, Denaro 1972) also share similarities. Karyotype I of Physalaemus petersi (Lourenco et al. 1999) re- sembles that of E. perezi, although the former has heteromorphic sex chromosomes which are ab- sent in E. perezi. In contrast, karyotype II of P petersi (Lourenco et al. 1999) differs considerably from that of E. perezi. 362 L. B. Lourenco, A. J. Cardoso and S. M. Recco-Pimentel Cytologia 65 The apparent divergence in the morphology of some chromosomes in the E. perezi karyotype and those of Pleurodema species (Barrio and Rinaldi de Chieri 1970, Duellman and Veloso 1977, Schmid et al. 1993) may reflect the different classification of these chromosome pairs based on their length. Thus, homologous chromosomes are probably identified by different numbers in differ- ent karyotypes. Alternatively, small changes in chromosome length may have resulted in some karyotypic divergence. On the other hand, the morphology of pair 8 differs greatly between E. perezi and P brachyops (Schmid et al. 1993). A comparative analysis of E. perezi and Physalaemus karyotypes, except for karyotype II of P petersi, revealed smaller divergences in chromosome ordering by length than those observed be- tween E. perezi and Pleurodema karyotypes. However, there was again a clear difference in the morphology of pair 8 between the E. perezi karyotype and P petersi karyotype I (Lourenco et al. 1999). Silver staining showed that pair 8 was the only NOR-bearing pair in E. perezi. In the kary- otypes of Pleurodema species, except P brachyops, pair 8 also bears an NOR constriction (Barrio and Rinaldi de Chieri 1970, Duellman and Veloso 1977, Schmid et al. 1993). Otherwise, the intra- chromosomal localization of these sites apparently differs between E. perezi and those Pleurodema species karyotypes. The P petersi karyotype I studied by silver staining has one of its multiple NOR sites located on pair 8 (Lourenco et al. 1998). However, the very distinct morphology between this pair and pair 8 of E. perezi precludes the recognition of any homology between these chromosomes. C-band analyses of E. perezi could not reveal any chromosomal homologies between this kary- otype and those of Pleurodema and Physalaernus species already studied by this technique (Schmid et al. 1993, Lourenco et al. 1999). Probably more useful information can arise after the cytogenetic study of a greater number of species of these genera. Acknowledgements This work was supported by the Brazilian agency CNPq (Conselho Nacional de Desenvolvi- mento Cientifico e Tecnologico). References Barrio, A. and Rinaldi de Chieri, P. 1970. Estudios citogeneticos sobre el genero Pleurodema y sus consecencias evolutivas (Amphibia, Anura, Leptodactylidae). Physis 30: 309-319. Batistic, R. F., Becak, M. L. and Vizotto, L. D. 1969. Variacao cromosomica no género Pseudopaludicola (Anura). Ciéncia e Cultura 21: 260. Becak, M. L. 1968. Chromosomal analysis of eighteen species of Anura. Caryologia 21: 191-208. ― , Denaro, L. and Becak, W. 1970. Polyploidy and mechanisms of karyotypic deversification in Amphibia. Cytogenetics 9: 225-238. Bogart, J. P. 1970. Systematics problems in the amphibian family Leptodactylidae (Anura) as indicated by karyotypic analy- sis. Cytogenetics 9: 369-383. •\ 1973. Evolution of Amuran Karyotypes. In: Vial J. L. (ed.). Evolutionary Biology of the Anurans: Contemporary Re- search on Major Problems. Univ. Missouri Press, Columbia. pp. 337-349. 1976. Ethology, karyology and taxonomic position of the South American•\ leptodactylid frog genus Edalorhina. Her- petol. Rev. 7: 75. De Lucca, E., Jim, J. and Foresti, F 1974. Chromosomal studies in twelve species of Leptodactylidae and one Brachy- cephalidae. Caryologia 27: 183-192. Denaro, L. 1972. Karyotypes of Leptodactylidae anurans. J. Herpetol. 6: 71-74. Duellman, W E. and Morales, V R. 1990. Variation, distribution, and life history of Edalorhina perezi (Amphibia, Anura, Leptodactylidae). Stud. Neotrop. Fauna Environ. 25: 19-30. and Veloso, M. A. 1977. Phylogeny of Pleurodema (Anura: •\ Leptodactylidae): a biogeographic model. Occas. Papers Mus. Nat. Hist. Univ. Kansas 64: 1-46.
Load more

Recommended publications
  • Plano De Manejo Da Reserva Biológica Nascentes Da Serra Do Cachimbo
    Apresentação Plano de Manejo da Reserva Biológica Nascentes da Serra do Cachimbo 2009 MINISTÉRIO DO MEIO AMBIENTE INSTITUTO CHICO MENDES DE CONSERVAÇÃO DA BIODIVERSIDADE DIRETORIA DE UNIDADES DE CONSERVAÇÃO DE PROTEÇÃO INTEGRAL PLANO DE MANEJO DA RESERVA BIOLÓGICA NASCENTES DA SERRA DO CACHIMBO APRESENTAÇÃO Brasília, 2009 PRESIDÊNCIA DA REPÚBLICA Luis Inácio Lula da Silva MINISTÉRIO DO MEIO AMBIENTE - MMA Carlos Minc Baumfeld - Ministro INSTITUTO CHICO MENDES DE CONSERVAÇÃO DA BIODIVERSIDADE - ICMBio Rômulo José Fernandes Mello - Presidente DIRETORIA DE UNIDADES DE CONSERVAÇÃO DE PROTEÇÃO INTEGRAL – DIREP Ricardo José Soavinski - Diretor COORDENAÇÃO GERAL DE UNIDADES DE CONSERVAÇÃO DE PROTEÇÃO INTEGRAL Maria Iolita Bampi - Coordenador COORDENAÇÃO DE PLANO DE MANEJO – CPLAM Carlos Henrique Fernandes - Coordenador COORDENAÇÃO DO BIOMA AMAZÔNIA - COBAM Lilian Hangae - Coordenador Brasília, 2009 CRÉDITOS TÉCNICOS E INSTITUCIONAIS Equipe de Elaboração do Plano de Manejo da Reserva Biológica Nascentes da Serra do Cachimbo Coordenação Geral Gustavo Vasconcellos Irgang – Instituto Centro de Vida - ICV Coordenação Técnica Jane M. de O. Vasconcellos – Instituto Centro de Vida Marisete Catapan – WWF Brasil Coordenação da Avaliação Ecológica Rápida Jan Karel Felix Mähler Junior Supervisão e Acompanhamento Técnico do ICMBio Allan Razera e Lílian Hangae Equipe de Consultores Responsáveis pelas Áreas Temáticas Meio Físico Gustavo Vasconcellos Irgang Roberta Roxilene dos Santos Jean Carlo Correa Figueira Vegetação Marcos Eduardo G. Sobral Ayslaner Victor
    [Show full text]
  • Catalogue of the Amphibians of Venezuela: Illustrated and Annotated Species List, Distribution, and Conservation 1,2César L
    Mannophryne vulcano, Male carrying tadpoles. El Ávila (Parque Nacional Guairarepano), Distrito Federal. Photo: Jose Vieira. We want to dedicate this work to some outstanding individuals who encouraged us, directly or indirectly, and are no longer with us. They were colleagues and close friends, and their friendship will remain for years to come. César Molina Rodríguez (1960–2015) Erik Arrieta Márquez (1978–2008) Jose Ayarzagüena Sanz (1952–2011) Saúl Gutiérrez Eljuri (1960–2012) Juan Rivero (1923–2014) Luis Scott (1948–2011) Marco Natera Mumaw (1972–2010) Official journal website: Amphibian & Reptile Conservation amphibian-reptile-conservation.org 13(1) [Special Section]: 1–198 (e180). Catalogue of the amphibians of Venezuela: Illustrated and annotated species list, distribution, and conservation 1,2César L. Barrio-Amorós, 3,4Fernando J. M. Rojas-Runjaic, and 5J. Celsa Señaris 1Fundación AndígenA, Apartado Postal 210, Mérida, VENEZUELA 2Current address: Doc Frog Expeditions, Uvita de Osa, COSTA RICA 3Fundación La Salle de Ciencias Naturales, Museo de Historia Natural La Salle, Apartado Postal 1930, Caracas 1010-A, VENEZUELA 4Current address: Pontifícia Universidade Católica do Río Grande do Sul (PUCRS), Laboratório de Sistemática de Vertebrados, Av. Ipiranga 6681, Porto Alegre, RS 90619–900, BRAZIL 5Instituto Venezolano de Investigaciones Científicas, Altos de Pipe, apartado 20632, Caracas 1020, VENEZUELA Abstract.—Presented is an annotated checklist of the amphibians of Venezuela, current as of December 2018. The last comprehensive list (Barrio-Amorós 2009c) included a total of 333 species, while the current catalogue lists 387 species (370 anurans, 10 caecilians, and seven salamanders), including 28 species not yet described or properly identified. Fifty species and four genera are added to the previous list, 25 species are deleted, and 47 experienced nomenclatural changes.
    [Show full text]
  • Biology and Impacts of Pacific Island Invasive Species. 8
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln USDA National Wildlife Research Center - Staff U.S. Department of Agriculture: Animal and Publications Plant Health Inspection Service 2012 Biology and Impacts of Pacific Island Invasive Species. 8. Eleutherodactylus planirostris, the Greenhouse Frog (Anura: Eleutherodactylidae) Christina A. Olson Utah State University, [email protected] Karen H. Beard Utah State University, [email protected] William C. Pitt National Wildlife Research Center, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/icwdm_usdanwrc Olson, Christina A.; Beard, Karen H.; and Pitt, William C., "Biology and Impacts of Pacific Island Invasive Species. 8. Eleutherodactylus planirostris, the Greenhouse Frog (Anura: Eleutherodactylidae)" (2012). USDA National Wildlife Research Center - Staff Publications. 1174. https://digitalcommons.unl.edu/icwdm_usdanwrc/1174 This Article is brought to you for free and open access by the U.S. Department of Agriculture: Animal and Plant Health Inspection Service at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in USDA National Wildlife Research Center - Staff Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Biology and Impacts of Pacific Island Invasive Species. 8. Eleutherodactylus planirostris, the Greenhouse Frog (Anura: Eleutherodactylidae)1 Christina A. Olson,2 Karen H. Beard,2,4 and William C. Pitt 3 Abstract: The greenhouse frog, Eleutherodactylus planirostris, is a direct- developing (i.e., no aquatic stage) frog native to Cuba and the Bahamas. It was introduced to Hawai‘i via nursery plants in the early 1990s and then subsequently from Hawai‘i to Guam in 2003. The greenhouse frog is now widespread on five Hawaiian Islands and Guam.
    [Show full text]
  • Species Diversity and Conservation Status of Amphibians in Madre De Dios, Southern Peru
    Herpetological Conservation and Biology 4(1):14-29 Submitted: 18 December 2007; Accepted: 4 August 2008 SPECIES DIVERSITY AND CONSERVATION STATUS OF AMPHIBIANS IN MADRE DE DIOS, SOUTHERN PERU 1,2 3 4,5 RUDOLF VON MAY , KAREN SIU-TING , JENNIFER M. JACOBS , MARGARITA MEDINA- 3 6 3,7 1 MÜLLER , GIUSEPPE GAGLIARDI , LILY O. RODRÍGUEZ , AND MAUREEN A. DONNELLY 1 Department of Biological Sciences, Florida International University, 11200 SW 8th Street, OE-167, Miami, Florida 33199, USA 2 Corresponding author, e-mail: [email protected] 3 Departamento de Herpetología, Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos, Avenida Arenales 1256, Lima 11, Perú 4 Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, California 94132, USA 5 Department of Entomology, California Academy of Sciences, 55 Music Concourse Drive, San Francisco, California 94118, USA 6 Departamento de Herpetología, Museo de Zoología de la Universidad Nacional de la Amazonía Peruana, Pebas 5ta cuadra, Iquitos, Perú 7 Programa de Desarrollo Rural Sostenible, Cooperación Técnica Alemana – GTZ, Calle Diecisiete 355, Lima 27, Perú ABSTRACT.—This study focuses on amphibian species diversity in the lowland Amazonian rainforest of southern Peru, and on the importance of protected and non-protected areas for maintaining amphibian assemblages in this region. We compared species lists from nine sites in the Madre de Dios region, five of which are in nationally recognized protected areas and four are outside the country’s protected area system. Los Amigos, occurring outside the protected area system, is the most species-rich locality included in our comparison.
    [Show full text]
  • Bibliography and Scientific Name Index to Amphibians
    lb BIBLIOGRAPHY AND SCIENTIFIC NAME INDEX TO AMPHIBIANS AND REPTILES IN THE PUBLICATIONS OF THE BIOLOGICAL SOCIETY OF WASHINGTON BULLETIN 1-8, 1918-1988 AND PROCEEDINGS 1-100, 1882-1987 fi pp ERNEST A. LINER Houma, Louisiana SMITHSONIAN HERPETOLOGICAL INFORMATION SERVICE NO. 92 1992 SMITHSONIAN HERPETOLOGICAL INFORMATION SERVICE The SHIS series publishes and distributes translations, bibliographies, indices, and similar items judged useful to individuals interested in the biology of amphibians and reptiles, but unlikely to be published in the normal technical journals. Single copies are distributed free to interested individuals. Libraries, herpetological associations, and research laboratories are invited to exchange their publications with the Division of Amphibians and Reptiles. We wish to encourage individuals to share their bibliographies, translations, etc. with other herpetologists through the SHIS series. If you have such items please contact George Zug for instructions on preparation and submission. Contributors receive 50 free copies. Please address all requests for copies and inquiries to George Zug, Division of Amphibians and Reptiles, National Museum of Natural History, Smithsonian Institution, Washington DC 20560 USA. Please include a self-addressed mailing label with requests. INTRODUCTION The present alphabetical listing by author (s) covers all papers bearing on herpetology that have appeared in Volume 1-100, 1882-1987, of the Proceedings of the Biological Society of Washington and the four numbers of the Bulletin series concerning reference to amphibians and reptiles. From Volume 1 through 82 (in part) , the articles were issued as separates with only the volume number, page numbers and year printed on each. Articles in Volume 82 (in part) through 89 were issued with volume number, article number, page numbers and year.
    [Show full text]
  • On the Uncertain Taxonomic Identity of Adenomera Hylaedactyla (Cope, 1868) and the Composite Type Series of A
    Copeia 107, No. 4, 2019, 708–723 On the Uncertain Taxonomic Identity of Adenomera hylaedactyla (Cope, 1868) and the Composite Type Series of A. andreae (Muller,¨ 1923) (Anura, Leptodactylidae) Thiago R. de Carvalho1, Ariovaldo A. Giaretta2, Natan M. Maciel3, Diego A. Barrera4,Cesar´ Aguilar-Puntriano4,5,Celio´ F. B. Haddad1, Marcelo N. C. Kokubum6, Marcelo Menin7, and Ariadne Angulo4,5 Adenomera andreae and A. hylaedactyla are two widespread Amazonian frogs that have been traditionally distinguished from each other by the use of different habitats, toe tip development, and more recently through advertisement calls. Yet, taxonomic identification of these species has always been challenging. Herein we undertake a review of type specimens and include new phenotypic (morphology and vocalization) and mitochondrial DNA information for an updated diagnosis of both species. Our morphological analysis indicates that the single type (holotype) of A. hylaedactyla could either belong to lineages associated with Amazonian forest-dwelling species (A. andreae clade) or to the open-formation morphotype (A. hylaedactyla clade). Given the holotype’s poor preservation, leading to the ambiguous assignment of character states for toe tip development, as well as a vague type locality encompassing a vast area in eastern Ecuador and northern Peru, the identity of this specimen is uncertain. Morphology of toe tip fragments and the original species description suggest that A. hylaedactyla could correspond to at least two described species (A. andreae or A. simonstuarti) or additional unnamed genetic lineages of the A. andreae clade, all bearing toe tips expanded into discs. Analysis of morphometric data, however, clustered the holotype with the Amazonian open-formation morphotype (toe tips unexpanded).
    [Show full text]
  • For Submission As a Note Green Anole (Anolis Carolinensis) Eggs
    For submission as a Note Green Anole (Anolis carolinensis) Eggs Associated with Nest Chambers of the Trap Jaw Ant, Odontomachus brunneus Christina L. Kwapich1 1Department of Biological Sciences, University of Massachusetts Lowell One University Ave., Lowell, Massachusetts, USA [email protected] Abstract Vertebrates occasionally deposit eggs in ant nests, but these associations are largely restricted to neotropical fungus farming ants in the tribe Attini. The subterranean chambers of ponerine ants have not previously been reported as nesting sites for squamates. The current study reports the occurrence of Green Anole (Anolis carolinensis) eggs and hatchlings in a nest of the trap jaw ant, Odontomachus brunneus. Hatching rates suggest that O. brunneus nests may be used communally by multiple females, which share spatial resources with another recently introduced Anolis species in their native range. This nesting strategy is placed in the context of known associations between frogs, snakes, legless worm lizards and ants. Introduction Subterranean ant nests are an attractive resource for vertebrates seeking well-defended cavities for their eggs. To access an ant nest, trespassers must work quickly or rely on adaptations that allow them to overcome the strict odor-recognition systems of ants. For example the myrmecophilous frog, Lithodytes lineatus, bears a chemical disguise that permits it to mate and deposit eggs deep inside the nests of the leafcutter ant, Atta cephalotes, without being bitten or harassed. Tadpoles inside nests enjoy the same physical and behavioral protection as the ants’ own brood, in a carefully controlled microclimate (de Lima Barros et al. 2016, Schlüter et al. 2009, Schlüter and Regös 1981, Schlüter and Regös 2005).
    [Show full text]
  • Restriction Fragment Analysis of the Ribosomal DNA of Paratelmatobius and Scythrophrys Species (Anura, Leptodactylidae)
    Genetics and Molecular Biology, 26, 2, 139-143 (2003) Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br Research Article Restriction fragment analysis of the ribosomal DNA of Paratelmatobius and Scythrophrys species (Anura, Leptodactylidae) Luciana B. Lourenço1, Paulo C.A. Garcia2 and Shirlei M. Recco-Pimentel1 1Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. 2Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Abstract Physical maps of the ribosomal RNA gene 28S of species belonging to the genera Paratelmatobius and Scythrophrys were constructed, using five restriction endonucleases. The restriction sites for Bam HI, Bgl II, Bst EII, and Eco RI had similar positions in all species, although there were interspecific differences in the size of the restriction fragments obtained. An additional Pvu II site was found in Scythrophrys specimens from Piraquara (State of Paraná, Brazil) and from São Bento do Sul (State of Santa Catarina, Brazil), but not in the Scythrophrys specimens from Rancho Queimado (State of Santa Catarina, Brazil). This finding is in agreement with the hypothesis regarding the existence of two species in the genus Scythrophrys. On the other hand, the extra Bst EII site considered in the literature to be a synapomorphy for the subfamilies Leptodactylinae and Telmatobiinae was not observed in the genera Paratelmatobius and Scythrophrys, which brings new questions about some taxonomic classifications that include Paratelmatobius in Leptodactylinae and Scythrophrys in Telmatobiinae. Interspecific variation was observed in the size of the restriction fragments analyzed and, in the case of group I Scythrophrys, there was also a variation between the individuals of the two populations.
    [Show full text]
  • Etar a Área De Distribuição Geográfica De Anfíbios Na Amazônia
    Universidade Federal do Amapá Pró-Reitoria de Pesquisa e Pós-Graduação Programa de Pós-Graduação em Biodiversidade Tropical Mestrado e Doutorado UNIFAP / EMBRAPA-AP / IEPA / CI-Brasil YURI BRENO DA SILVA E SILVA COMO A EXPANSÃO DE HIDRELÉTRICAS, PERDA FLORESTAL E MUDANÇAS CLIMÁTICAS AMEAÇAM A ÁREA DE DISTRIBUIÇÃO DE ANFÍBIOS NA AMAZÔNIA BRASILEIRA MACAPÁ, AP 2017 YURI BRENO DA SILVA E SILVA COMO A EXPANSÃO DE HIDRE LÉTRICAS, PERDA FLORESTAL E MUDANÇAS CLIMÁTICAS AMEAÇAM A ÁREA DE DISTRIBUIÇÃO DE ANFÍBIOS NA AMAZÔNIA BRASILEIRA Dissertação apresentada ao Programa de Pós-Graduação em Biodiversidade Tropical (PPGBIO) da Universidade Federal do Amapá, como requisito parcial à obtenção do título de Mestre em Biodiversidade Tropical. Orientador: Dra. Fernanda Michalski Co-Orientador: Dr. Rafael Loyola MACAPÁ, AP 2017 YURI BRENO DA SILVA E SILVA COMO A EXPANSÃO DE HIDRELÉTRICAS, PERDA FLORESTAL E MUDANÇAS CLIMÁTICAS AMEAÇAM A ÁREA DE DISTRIBUIÇÃO DE ANFÍBIOS NA AMAZÔNIA BRASILEIRA _________________________________________ Dra. Fernanda Michalski Universidade Federal do Amapá (UNIFAP) _________________________________________ Dr. Rafael Loyola Universidade Federal de Goiás (UFG) ____________________________________________ Alexandro Cezar Florentino Universidade Federal do Amapá (UNIFAP) ____________________________________________ Admilson Moreira Torres Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá (IEPA) Aprovada em de de , Macapá, AP, Brasil À minha família, meus amigos, meu amor e ao meu pequeno Sebastião. AGRADECIMENTOS Agradeço a CAPES pela conceção de uma bolsa durante os dois anos de mestrado, ao Programa de Pós-Graduação em Biodiversidade Tropical (PPGBio) pelo apoio logístico durante a pesquisa realizada. Obrigado aos professores do PPGBio por todo o conhecimento compartilhado. Agradeço aos Doutores, membros da banca avaliadora, pelas críticas e contribuições construtivas ao trabalho.
    [Show full text]
  • Foam Nest Construction and First Report of Agonistic Behaviour In
    Neotropical Biology and Conservation 14(1): 117–128 (2019) doi: 10.3897/neotropical.14.e34841 SHORT COMMUNICATION Foam nest construction and first report of agonistic behaviour in Pleurodema tucumanum (Anura, Leptodactylidae) Melina J. Rodriguez Muñoz1,2, Tomás A. Martínez1,2, Juan Carlos Acosta1, Graciela M. Blanco1 1 Gabinete DIBIOVA (Diversidad y Biología de Vertebrados del Árido). Departamento de Biología, FCEFN, Universidad Nacional de San Juan, Avenida Ignacio de la Roza 590, Rivadavia J5400DCS, San Juan, Argentina 2 Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, C1425FQB, Ciudad Autónoma de Buenos Aires Argentina Corresponding author: Melina J. Rodriguez Muñoz ([email protected]) Academic editor: A. M. Leal-Zanchet | Received 21 May 2018 | Accepted 27 December 2018 | Published 11 April 2019 Citation: Rodriguez Muñoz MJ, Martínez TA, Acosta JC, Blanco GM (2019) Foam nest construction and first report of agonistic behaviour in Pleurodema tucumanum (Anura: Leptodactylidae). Neotropical Biology and Conservation, 14(1): 117–128. https://doi.org/10.3897/neotropical.14.e34841 Abstract Reproductive strategies are the combination of physiological, morphological, and behavioural traits interacting to increase species reproductive success within a set of environmental conditions. While the reproductive strategies of Leiuperinae are known, few studies have been conducted regarding the reproductive behaviour that underlies them. The aim of this study was to document the structural characteristics of nesting microsites, to describe the process of foam nest construction, and to explore the presence of male agonistic and chorus behaviour in Pleurodema tucumanum. Nests were found close to the edge of a temporary pond and the mean temperature of the foam nests was always close to the mean temperature of the pond water.
    [Show full text]
  • Natural History Notes 643
    NATURAL HISTORY NOTES 643 Programa de Pós-Graduação em Ciências Biológicas (Zoologia), Labo- Amazonas, Brazil (e-mail: [email protected]); ALBERTINA P. LIMA, De- ratório/Coleção de Herpetologia, Universidade Federal da Paraíba, Cidade partamento de Ecologia, Instituto Nacional de Pesquisas da Amazônia – Universitária, Campus I, CEP 58059-900, João Pessoa, Paraíba, Brazil; JEN- Campus V8, Av. Efigênio Sales, 2239, 69060-020, Manaus, Amazonas, Brazil NIFER KATIA RODRIGUES, Department of Biology, Universidade Region- (e-mail: [email protected]). al do Cariri, Rua Cel. Antonio Luiz, s/n, Bairro-Pimenta, Crato-Ceará, Brazil. MICROHYLA BUTLERI (Tubercled Pygmy Frog). PREDATION. At LITHODYTES LINEATUS (Painted Antnest Frog). ASSOCIATION 2112 h on 15 August 2008, at the Xishuangbanna Tropical Botanic WITH ATTA ANTS. The first report showing the association be- Garden (21.92906°N, 101.25269°E, WGS 84), Yunnan Province, tween Lithodytes lineatus and leaf-cutting ants of the genus Atta China, we observed the female (bottom member) of a breeding was described by Schlüter (1980. Salamandra 164:227–247) who heard individuals vocalizing inside the galleries of these ants. Later, other observations involving the association between these genera were published and, until now, involved L. lineatus using active nests of Atta cephalotes to vocalize and also as breeding sites HELMUS R. M. BY (Schlüter and Rëgos 1981. Amphibia-Reptilia 2:117–121; Lamar PHOTO and Wild 1995. Herpetol. Nat. Hist. 32:135–142; Schlüter et al. 2009. Herpetol. Notes 2:101–105). However, the taxonomic identities of other species of Atta that L. lineatus associates with have not yet been described. We conducted active searches to find L.
    [Show full text]
  • Chromosome Analysis of Five Brazilian
    c Indian Academy of Sciences RESEARCH ARTICLE Chromosome analysis of five Brazilian species of poison frogs (Anura: Dendrobatidae) PAULA CAMARGO RODRIGUES1, ODAIR AGUIAR2, FLÁVIA SERPIERI1, ALBERTINA PIMENTEL LIMA3, MASAO UETANEBARO4 and SHIRLEI MARIA RECCO-PIMENTEL1∗ 1Departamento de Anatomia, Biologia Celular e Fisiologia, Instituto de Biologia, Universidade Estadual de Campinas, 13083-863 Campinas, São Paulo, Brazil 2Departamento de Biociências, Universidade Federal de São Paulo, Campus Baixada Santista, 11060-001 Santos, São Paulo, Brazil 3Coordenadoria de Pesquisas em Ecologia, Instituto Nacional de Pesquisas do Amazonas, 69011-970 Manaus, Amazonas, Brazil 4Departamento de Biologia, Universidade Federal de Mato Grosso do Sul, 70070-900 Campo Grande, Mato Grosso do Sul, Brazil Abstract Dendrobatid frogs have undergone an extensive systematic reorganization based on recent molecular findings. The present work describes karyotypes of the Brazilian species Adelphobates castaneoticus, A. quinquevittatus, Ameerega picta, A. galactonotus and Dendrobates tinctorius which were compared to each other and with previously described related species. All karyotypes consisted of 2n = 18 chromosomes, except for A. picta which had 2n = 24. The karyotypes of the Adelphobates and D. tinctorius species were highly similar to each other and to the other 2n = 18 previously studied species, revealing conserved karyotypic characteristics in both genera. In recent phylogenetic studies, all Adelphobates species were grouped in a clade separated from the Dendrobates species. Thus, we hypothesized that their common karyotypic traits may have a distinct origin by chromosome rearrangements and mutations. In A. picta, with 2n = 24, chromosome features of pairs from 1 to 8 are shared with other previously karyotyped species within this genus. Hence, the A.
    [Show full text]