The Eye Examination 1
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Prevention of Traumatic Corneal Ulcer in South East Asia
FROM OUR SOUTH ASIA EDITION Prevention of traumatic corneal ulcer in South East Asia S C AE Srinivasan/ (c)M Country Principal Investigator and Lead Principal Investigator with village health workers in Bhutan Dr. M. Srinivasan ciasis, and leprosy, are declining, and (VVHW) of the Government were utilized Director Emeritus, Aravind Eye Care, soon the majority of corneal blindness will to identify ocular injury and treat corneal Madurai, Tamil Nadu India. be due to microbial keratitis. Most abrasion corneal ulcers occur among agricultural Myanmar: Village Health Workers (VHW) workers in developing countries following of the health department Introduction corneal abrasion. India: paid village volunteers were utilized Corneal ulceration is a leading cause of Several non-randomized prevention visual impairment globally, with a dispro- studies conducted before 2000 Inclusion criteria 2 portionate burden in developing (Bhaktapur Eye Study) and during 2002 • Resident of study area countries. It was estimated that 6 million to 2004 in India, Myanmar, and Bhutan • Corneal abrasion after ocular injury, corneal ulcers occur annually in the ten by World Health Organization(WHO), have confirmed by clinical examination with countries of South East Asia Region suggested that antibiotic ointment fluorescein stain and a blue torch encompassing a total population of 1.6 applied promptly after a corneal abrasion • Reported within 48 hours of the injury billion.1 While antimicrobial treatment is could lower the incidence of ulcers, • Subject aged >5 years of age generally effective in treating infection, relative to neighbouring or historic “successful” treatment is often controls.3-4 Prevention of traumatic Exclusion criteria associated with a poor visual outcome. -
Wildlife Ophthalmology
Wildlife Ophthalmology DR. HEATHER REID TORONTO WILDLIFE CENTRE TORONTO, ON CANADA Why understand eyes? Wildlife need to have excellent vision to survive in the wild Eye related problems are common in wildlife admitted to rehabilitation centers What we will cover Anatomy of the eye Differences between birds and mammals The eye exam Recognizing common problems Prognosis Treatment options When to see the vet Anatomy Around the Eye: Muscles & nerves Skin Eye lids Nictitating eyelid Conjunctiva & sclera Tear glands & ducts Ossicles (birds) Anatomy Front of the Eye: Cornea Iris Pupil Ciliary body Anterior Chamber Aqueous humor Anatomy Back of the Eye: Lens Retina Optic nerve Choroid Pecten (birds) Posterior Chamber Vitreous humor Fundus of the Eye Mammal Eye Bird Eye The Avian Eye - Differences Small eye size in most birds and small pupil size makes it hard to examine Can control the size of their pupil Lower eyelid more developed The nictitating membrane spreads the tears allowing birds to blink less Moves horizontally across eye The Avian Eye - Differences Eyes are not as protected by skull Less muscles around eye so less eye movement Boney ossicles support the eye Three main eye shapes; flat, globose & tubular The Avian Eye - Differences Four different color receptors compared to the three in mammals means better color detail Can see in the ultraviolet range Higher flicker rate – can detect lights that flicker at more than 100 flashes per second (humans detect at 50) The Avian Eye - Differences In some species the eye -
Optic Disc Edema, Globe Flattening, Choroidal Folds, and Hyperopic Shifts Observed in Astronauts After Long-Duration Space Flight
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln NASA Publications National Aeronautics and Space Administration 10-2011 Optic Disc Edema, Globe Flattening, Choroidal Folds, and Hyperopic Shifts Observed in Astronauts after Long-duration Space Flight Thomas H. Mader Alaska Native Medical Center, [email protected] C. Robert Gibson Coastal Eye Associates Anastas F. Pass University of Houston Larry A. Kramer University of Texas Health Science Center Andrew G. Lee The Methodist Hospital See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/nasapub Part of the Physical Sciences and Mathematics Commons Mader, Thomas H.; Gibson, C. Robert; Pass, Anastas F.; Kramer, Larry A.; Lee, Andrew G.; Fogarty, Jennifer; Tarver, William J.; Dervay, Joseph P.; Hamilton, Douglas R.; Sargsyan, Ashot; Phillips, John L.; Tran, Duc; Lipsky, William; Choi, Jung; Stern, Claudia; Kuyumjian, Raffi; andolk, P James D., "Optic Disc Edema, Globe Flattening, Choroidal Folds, and Hyperopic Shifts Observed in Astronauts after Long-duration Space Flight" (2011). NASA Publications. 69. https://digitalcommons.unl.edu/nasapub/69 This Article is brought to you for free and open access by the National Aeronautics and Space Administration at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in NASA Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Thomas H. Mader, C. Robert Gibson, Anastas F. Pass, Larry A. -
Cultivating a Cure for Blindness
news and views were taken in a l-mm2 biopsy from the good eye, then they were cultured and, on second Cultivating a cure passage, they formed a tightly packed and communicating (confluent) monolayer of cells. For grafting, the conjunctiva! epi for blindness thelium was completely removed from the cornea and limbus of the recipient eye, and replaced with a slightly larger monolayer of Stuart Hodson cultured limbal epithelium. The eyes were Damaged corneas can often be repaired using donor grafts, but if the then covered with therapeutic soft contact damage is too great the graft wlll be rejected. This may change with the lenses, and tightly patched for several days. development of a method to Inhibit rejection which uses cultivated cells. The results after the limbal epithelial graft were very promising. The grafted epithelium he human cornea has a special proper epithelium can mould a smooth apical and was stable, transparent, multi-layered and Tty known as immune privilege, which basal surface, the limbal epithelial stem cells smooth. One of the patients had suffered an allows tissue grafts from donors to be do not form such a smooth surface. alkali burn to his left eye ten years earlier, and carried out without the usual problems of If the corneal epithelium is lost it can be had undergone three previous unsuccessful immune rejection. However, immune privi functionally regenerated by the limbal stem corneal grafts. Before the treatment he had lege is lost at the perimeter of the cornea - cells. But if both the corneal and limbal continual severe corneal vascularization the limb us of the eye - where the transpar epithelia are lost, the corneal surface is re ( development of blood vessels) and persis ent corneal stroma meets the opaque sclera colonized by the other neighbour of the tent ulceration, and the eye was painful and (Fig. -
Quantitative Assessment of Central and Limbal Epithelium After Long
Eye (2016) 30, 979–986 © 2016 Macmillan Publishers Limited All rights reserved 0950-222X/16 www.nature.com/eye 1,5 1,5 1 Quantitative RK Prakasam , BS Kowtharapu , K Falke , CLINICAL STUDY K Winter2,3, D Diedrich4, A Glass4, A Jünemann1, assessment of central RF Guthoff1 and O Stachs1 and limbal epithelium after long-term wear of soft contact lenses and in patients with dry eyes: a pilot study Abstract Purpose Analysis of microstructural Eye (2016) 30, 979–986; doi:10.1038/eye.2016.58; alterations of corneal and limbal epithelial published online 22 April 2016 cells in healthy human corneas and in other ocular conditions. Introduction Patients and methods Unilateral eyes of three groups of subjects include healthy The X, Y, Z hypothesis1 explains cell mechanism volunteers (G1, n = 5), contact lens wearers that is essential for the renewal and maintenance 1Department of (G2, n = 5), and patients with dry eyes of the corneal epithelium. This hypothesis Ophthalmology, University = proposes that the loss of corneal epithelial of Rostock, Rostock, (G3, n 5) were studied. Imaging of basal Germany (BC) and intermediate (IC) epithelial cells surface cells (Z) can be maintained by the from central cornea (CC), corneal limbus proliferation of basal epithelial cells (X), and the 2Faculty of Medicine, centripetal movements of the peripheral (CL) and scleral limbus (SL) was obtained by Institute of Anatomy, epithelial cells (Y). By utilizing this mechanism, University of Leipzig, in vivo confocal microscopy (IVCM). An it is also possible to categorize both disease and Leipzig, Germany appropriate image analysis algorithm was therapies according to the specific component 3 used to quantify morphometric parameters involved.1 Therefore it is vital to understand the Institute for Medical including mean cell area, compactness, Informatics, Statistics and cellular structures of both central and limbal Epidemiology (IMISE), solidity, major and minor diameter, and epithelial cells in normal and in various corneal University of Leipzig, maximum boundary distance. -
Development of in Vitro Corneal Models: Opportunity for Pharmacological Testing
Review Development of In Vitro Corneal Models: Opportunity for Pharmacological Testing Valentina Citi 1, Eugenia Piragine 1, Simone Brogi 1,* , Sara Ottino 2 and Vincenzo Calderone 1 1 Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy; [email protected] (V.C.); [email protected] (E.P.); [email protected] (V.C.) 2 Farmigea S.p.A., Via G.B. Oliva 6/8, 56121 Pisa, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-050-2219-613 Received: 24 October 2020; Accepted: 30 October 2020; Published: 2 November 2020 Abstract: The human eye is a specialized organ with a complex anatomy and physiology, because it is characterized by different cell types with specific physiological functions. Given the complexity of the eye, ocular tissues are finely organized and orchestrated. In the last few years, many in vitro models have been developed in order to meet the 3Rs principle (Replacement, Reduction and Refinement) for eye toxicity testing. This procedure is highly necessary to ensure that the risks associated with ophthalmic products meet appropriate safety criteria. In vitro preclinical testing is now a well-established practice of significant importance for evaluating the efficacy and safety of cosmetic, pharmaceutical, and nutraceutical products. Along with in vitro testing, also computational procedures, herein described, for evaluating the pharmacological profile of potential ocular drug candidates including their toxicity, are in rapid expansion. In this review, the ocular cell types and functionality are described, providing an overview about the scientific challenge for the development of three-dimensional (3D) in vitro models. -
Physical Eye Examination
Physical Eye Examination Kaevalin Lekhanont, MD Department of Ophthalmology Ramathibodi Hospp,ital, Mahidol Universit y Outline • Visual acuity (VA) testing – Distant VA test – Pinhole test – Near VA test • Visual field testing • Record and interpretations Outline • Penlight examination •Swingggping penli ght test • Direct ophthalmoscopy – Red reflex examination • Schiotz tonometry • RdditttiRecord and interpretations Conjunctiva, Sclera Retina Cornea Iris Retinal blood vessels Fovea Pupil AtAnteri or c ham ber Vitreous Aqueous humor Lens Optic nerve Trabecular meshwork Ciliary body Choriod and RPE Function evaluation • Visual function – Visual acuity test – Visual field test – Refraction • Motility function Anatomical evaluation Visual acuity test • Distant VA test • Near VA test Distance VA test Snellen’s chart • 20 ฟุตหรือ 6 เมตร • วัดที่ละขาง ตาขวากอนตาซาย • ออานทละตาานทีละตา แถวบนลงลแถวบนลงลางาง • บันทึกแถวลางสุดที่อานได Pinhole test VA with pinhole (PH) Refractive error emmetitropia myypopia hyperopia VA record 20/200 ผูปวยสามารถอานต ัวเลขทมี่ ี ขนาดใหญขนาดใหญพอทคนปกตพอที่คนปกติ สามารถอานไดจากท ี่ระยะ 200 ฟตฟุต แตแตผผปูปวยอานไดจากวยอานไดจาก ที่ระยะ 20 ฟุต 20/20 Distance VA test • ถาอานแถวบนสุดไไไมได ใหเดินเขาใกล chthart ทีละกาวจนอานได (10/200, 5/200) • Counting finger 2ft - 1ft - 1/2ft • Hand motion • Light projection • Light perception • No light perception (NLP) ETDRS Chart Most accurate Illiterate E chart For children age ≥ 3.5 year Near VA test Near chart •14 นวิ้ หรอื 33 เซนตเมตริ • วัดที่ละขาง ตาขวากอนตาซาย • อานทีละตา แถวบนลงลาง -
Examination of The
Colors and Eye Examination Techniques in Horses Equine Ophthalmology Service University of Florida There are really only 3 ophthalmic diseases!! 1. Corneal ulcers 2. Uveitis 3. Everything else!! Heine C-002-14-400 Heine C-002.14.602 Obvious Things Just stand back and look at the symmetry – Lashes – Discomfort and squinting – Tearing – Colors – Pupil – Clarity of cornea and lens – The normal eye is “shiny” – Anatomy: anterior to posterior 95677 Champagne RMH Lashes pointing down can be early Lashes sign of eye pain (ponies can look through their lashes) Nuclear Sclerosis Ocular Discomfort Level 107656 Dusty UF Res Corneal “Colors” White cornea: abscess or necrosis Blue cornea: edema Red cornea: vessels – Superficial (tree-like) and deep vessels (brush). – Note intensity of the red Dark is thin Shiny is thin Vascular Patterns Redness – Very Red – Pale Symmetry – Asymmetry 189711 154841 194013 Asymmetrical vascularization Callie Edema Corneal Haze – Endothelial – Uveitis Subepithelial scar Corneal abrasion/ulcer Subepithelial inflitrate – Immune mediated Fly – Fungal Mount Oakely IMMK Shooter Epithelial edema Sunshine Chief Barber IMMK Jupiter: DSA Chronic Recurrent Deep Immune Mediated Keratitis Green fluid filled lacunae form in the stroma “Alexiej” May SEK: fungi Candleabra SEK Pupil Size Dilated – Glaucoma – Retinal Disease – Optic Nerve Disease Miotic – Uveitis Deep corneal scrapings at the edge of the ulcer to detect bacteria and fungal hyphae Superficial swabbing cannot be expected to yield microbes in a high percentage of cases. Scrape with handle end of scalpel blade. Fluorescein: Every eye exhibiting signs of pain should be stained!! – Detects a corneal epithelial defect or “ulcer”. – Cobalt blue filter aids detection of abrasions. -
Smartphone-Based Dilated Fundus Photography and Near Visual Acuity Testing As Inexpensive Screening Tools to Detect Referral Warranted Diabetic Eye Disease
Smartphone-Based Dilated Fundus Photography and Near Visual Acuity Testing as Inexpensive Screening Tools to Detect Referral Warranted Diabetic Eye Disease BRIAN C. TOY, MD,*† DAVID J. MYUNG, MD, PHD,*† LINGMIN HE, MD,*† CAROLYN K. PAN, MD,*† ROBERT T. CHANG, MD,* ALISON POLKINHORNE, MA,‡ DOUGLAS MERRELL, BS,‡ DOUG FOSTER, MBA,‡ MARK S. BLUMENKRANZ, MD* Purpose: To compare clinical assessment of diabetic eye disease by standard dilated examination with data gathered using a smartphone-based store-and-forward teleoph- thalmology platform. Methods: 100 eyes of 50 adult patients with diabetes from a health care safety-net ophthalmology clinic. All patients underwent comprehensive ophthalmic examination. Concurrently, a smartphone was used to estimate near visual acuity and capture anterior and dilated posterior segment photographs, which underwent masked, standardized review. Quantitative comparison of clinic and smartphone-based data using descriptive, kappa, Bland-Altman, and receiver operating characteristic analyses was performed. Results: Smartphone visual acuity was successfully measured in all eyes. Anterior and posterior segment photography was of sufficient quality to grade in 96 and 98 eyes, respectively. There was good correlation between clinical Snellen and smartphone visual acuity measurements (rho = 0.91). Smartphone-acquired fundus photographs demon- strated 91% sensitivity and 99% specificity to detect moderate nonproliferative and worse diabetic retinopathy, with good agreement between clinic and photograph grades (kappa = 0.91 ± 0.1, P , 0.001; AUROC = 0.97, 95% confidence interval, 0.93–1). Conclusion: The authors report a smartphone-based telemedicine system that demon- strated sensitivity and specificity to detect referral-warranted diabetic eye disease as a proof-of-concept. Additional studies are warranted to evaluate this approach to expanding screening for diabetic retinopathy. -
Management of Hemorrhagic Choroidal Detachment by Thomas Albini, MD; John Kitchens, MD; Jonathan Prenner, MD; Charles Mango, MD; and Andrew Moshfeghi, MD, MBA
RETINA SURGERY SURGICAL UPDATES Section Co-Editors: Rohit Ross Lakhanpal, MD; and Jorge A. Fortun, MD A print & video series from the Vit-Buckle Society eyetube.net Management of Hemorrhagic Choroidal Detachment BY THOMAS ALBINI, MD; JOHN KITCHENS, MD; JONATHAN PRENNER, MD; CHARLES MANGO, MD; AND ANDREW MOSHFEGHI, MD, MBA emorrhagic choroidal detachment can be an unfortunate complication of ophthalmic surgery with significant ocular morbidity. Often, vitreo- retinal surgeons are involved in the management Hof such cases; however, evidence to support a standardized approach to the treatment strategy or surgical drainage techniques is not well established. In this month’s discus- sion, a panel of Vit-Buckle Society (VBS) members answers “In appositional choroidal key questions regarding their approaches to the manage- detachments, I will make the ment of this often challenging condition. Our esteemed decision to drain if there is no panel consists of VBS members Thomas Albini, MD; Jonathan Prenner, MD; John Kitchens, MD; Charles Mango, resolution within 1 week.” MD; and Andrew Moshfeghi, MD, MBA. -Charles Mango, MD Are there any medical treatments that you have found helpful before proceeding with What are your indications for proceeding surgical intervention? with drainage of a hemorrhagic choroidal Dr. Prenner: I tend to place my choroidal detachment detachment? patients on 4 times daily atropine and difluprednate Dr. Prenner: I perform drainage when the choroidal (Durezol, Alcon Laboratories, Inc.). detachment results in retinal apposition or angle closure with an elevated intraocular pressure (IOP). Dr. Kitchens: I find that use of oral steroids (predini- sone 40-60 mg daily for 1 week followed by a taper) Dr. -
Semaphorin3a/Neuropilin-1 Signaling Acts As a Molecular Switch Regulating Neural Crest Migration During Cornea Development
Developmental Biology 336 (2009) 257–265 Contents lists available at ScienceDirect Developmental Biology journal homepage: www.elsevier.com/developmentalbiology Semaphorin3A/neuropilin-1 signaling acts as a molecular switch regulating neural crest migration during cornea development Peter Y. Lwigale a,⁎, Marianne Bronner-Fraser b a Department of Biochemistry and Cell Biology, MS 140, Rice University, P.O. Box 1892, Houston, TX 77251, USA b Division of Biology, 139-74, California Institute of Technology, Pasadena, CA 91125, USA article info abstract Article history: Cranial neural crest cells migrate into the periocular region and later contribute to various ocular tissues Received for publication 2 April 2009 including the cornea, ciliary body and iris. After reaching the eye, they initially pause before migrating over Revised 11 September 2009 the lens to form the cornea. Interestingly, removal of the lens leads to premature invasion and abnormal Accepted 6 October 2009 differentiation of the cornea. In exploring the molecular mechanisms underlying this effect, we find that Available online 13 October 2009 semaphorin3A (Sema3A) is expressed in the lens placode and epithelium continuously throughout eye development. Interestingly, neuropilin-1 (Npn-1) is expressed by periocular neural crest but down- Keywords: Semaphorin3A regulated, in a manner independent of the lens, by the subpopulation that migrates into the eye and gives Neuropilin-1 rise to the cornea endothelium and stroma. In contrast, Npn-1 expressing neural crest cells remain in the Neural crest periocular region and contribute to the anterior uvea and ocular blood vessels. Introduction of a peptide that Cornea inhibits Sema3A/Npn-1 signaling results in premature entry of neural crest cells over the lens that Lens phenocopies lens ablation. -
Localisation of Corneal Epithelial Progenitors and Characterization of Cell-Cell Interactions in the Human Limbal Stem Cell Niche
Localisation of corneal epithelial progenitors and characterization of cell-cell interactions in the human limbal stem cell niche A thesis submitted for the degree of Doctor of Philosophy (PhD) University College London (UCL) 2015 Marc A. Dziasko Supervised by Professor Julie T. Daniels, PhD FSB Mr Stephen J. Tuft MA MChir MD FRCOphth Division of ORBIT (Ocular Biology and Therapeutics) UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL 1 Declaration I, Marc Alexandre Dziasko confirm that the work presented in this thesis is my own. Where information has been derived from other sources, I confirm that this has been referenced in the thesis. Name: Marc Alexandre DZIASKO Signature: Date: 18/09/2015 2 Abstract The cornea, the transparent tissue located at the front of the eye, is a highly specialized tissue that transmits and refracts light onto the retina. Maintenance of the corneal epithelium relies on a population of limbal epithelial stem cells (LESCs) that maintain transparency of the ocular surface that is essential for vision. Despite great advances in our understanding of ocular stem cell biology over the last decade, the exact location of the LESC niche remains unclear. After observing a high population of basal epithelial cells expressing stem cell markers within the previously identified limbal crypts (LC), the first aim of this study was to demonstrate by in vitro clonal analysis that these structures provide a niche for the resident LESCs. High-resolution transmission electron microscopy has been further used to image the basal epithelial layer at the limbus. Cells with morphology consistent with stem cells were present within the basal layer of the limbal crypts but not within the basal layer of non-crypt limbal biopsies.