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ACTH in Tetrahymena Pyriformis, 76 Adenylate Cyclase Inhibition By INDEX ACTH Aromatase in tetrahymena pyriformis, 76 activity, 469 Adenylate Cyclase Armatase activity inhibition by somatostatin, granulosa cells, 317 377 OS induced, 253 Administered LHRHA with testosterone enanthate, 598 Bacteria Adrenal androgens binding of TSH, 77 in prostatic cancer, 540 Blocking agents Agonist or antagonist of gonadotropin receptor, 222 binding to receptor, 242 Ca2+ Androgen insensitive cells interaction of receptor, 267 exposed to low androgen, 542 on intracellular events, 267 Androgen receptor blockers Calmodulin inhibitors cyproterone acetate, flutamide, block GnRH, 160 558 cAMP Angiotensin 11 activation of phospho­ PRL release, 359, 361 diesterase, 413 Anterior pituitary function cumulus-oocyte complex, 328 neuromodulators, 48 regulation of oocyte Antral f1uid maturation, 326 FSH, LH and prolactin, 320 cAMP accumulation steroid hormone concentrations, LH-induced, 333 319 oocyte maturation, 332 AP cells cAMP and 20a-OH-P information on membrane compart­ response to OS or FSH, 257 ments, 170 cAMP binding activity Arachidonic acid effects of FSH and LHRH, 473 action of LHRH, 6 cAMP levels Arachidonic acid (AA) pregnenolone production, 429 prostaglandins (PG), 494 cAMP phosphodieste~se Arachidonic acid conversion stimulated by Ca ,calmodulin, preovulatory follicles, 348 414 Arachidonic acid metabolites Casein secretion of LH, 6 encoded by complex split genes, Arachodonic metabolites 389 in ovulation, 344 gene structure, 389 611 612 INDEX 6 Casein mRNAs D-Arg analog sequences, 389 of LH-RH, 522 a-casein Deglycosylated gonadotropins phosphorylation site, 391 acute effects, 227 y-casein gene antagonists, 608 hormone binding sites, 392 desentization, 241 structure, 390 preparation, 224 Cell response regulation Demorphin phosphodiesterase, 417 LH, FSH secretion, 40 Cholesterol prolactin release, 43 for steroidogenic tissues, 292 Desensitization Cholesterol availability hormone-induced, 243 uptake of lipoproteins, 424 hormone-receptor complexes, 236 Cholesterol side-chain cleavage DG-gonadotropins regulation, 426 antagonistic activity, 229 Chromatography DGhCG and hCG degradation products of LHRH, cycSic AMP response, 239 119 D-Lys -GnRH CL function agonist dimer, 159 controlled by PRL, LH and immobile support, 154 placental lactogen, 291 Dopamine Collagen degradation release of LHRH, 7 cysteine, 342 Dopamine agonists Collagen degrading enzymes anterior pituitary cells, 375 follicular rupture, 338 Down regulation Copper continuous high concentrations regulation of LHRH release, LHRH, 608 35 low concentrations LHRH, 608 Corpora lutea up-regulation, 608 in culture, 281 estradiol treatment, 278 in organ culture, 278 E pr~.freatment progesterone and 20-dihydro- Ca -dependent protein phosphory­ progesterone, 279 lation, 301 Corpus luteum Endocrine and nervous system dependence of estradiol, 276 boundaries, 73 functional regulation, 264 a-endorphin antiserum regression, 268 elevated plasma LH, 511 Covalent linking Ergot alkaloids of photoreactive GnRH, 131 adenylate cyclase receptor, 374 Cumulus cells Estradiol uridine uptake, 334 inhibition of translation, 405 Cyclic AMP formation on luteal steroidogenesis, 296 effect of DGFSH and DghCG, 242 l7a estradiol Cycling female rats luteotrophic hormone, 274 serial fields, 186 luteotrophic hormone in rabbit, 28~ Cysteine Estradiol implant prevented ovulation, 340 serum progesterone, 277 Estradiol-treated rabbits progesterone, 275 INDEX 613 Estrogen FSH release luteal weight, 291 prostacyclin, 12 Estrogens FSH cell population cardiovascular side-effects, proestrous females, 193 542 FSHRF Estrous cycle activity of FSH, 20 adenohypophyseal muscarinic receptors, 57 Gene expression milk protein, 386 Flutamide GH3 cells antiandrogen, 541 secretory activity, 174 Follicle visualized, 172 dominant, 476 GnRH healthy and atretic, 310 antiserum treatment, 146 numbers of granulosa cells, binding by the receptor, 153 314 calmodulin, 160 microenvironment, 310 clinical applications, 564 morphologic and hormonal desensitization, 160 characterization, 312 fluorescent analogs, 154 peptide hormones, 318 gonadotroph differentiation, 147 progesterone/estradiol ratio, induction of ovulation, 566 321 internalization, 134 proteins in regulation, 476 mode of action, 563 Follicle-enclosed oocytes PA and P1 labeling, 497 maturation, 331 phospholipid in interstitial Follicular cAMP levels cells, 502 oocyte maturation, 331 phospholipid labeling in ovary, Follicular cells 502 Gap junctions, 201 photoaffinity derivative, 128 Follicular rupture pulsatile administration, 586, 537 arachidonic acid, 345 pulsatile induction of ovulation, collagenolysis, 338 576 lipoxygenase, 349 pulsatile therapy, 564 lipoxygenase products, 346 response system, 157 plasminogen activator, 341 rhodamine derivative, 133 proteolytic activity, 337 testicular P1 labeling, 500 Forskolin GnRHa cAMP response, 328 labeling of phospholipids, 499 cumulis-enclosed oocytes, 330 GnRH agonists FSH calcium dependent, 503 gastric inhibitory peptide, 16 no effect on cAMP, 501 separate hypothalamic control, granulosa and luteal cells, 202 19 GnRH and analogs FSHS structures, 128 l5-base oligomer, 402 GnRH and LH FSH and LH release stimulation 9f ovarian functions, effects of various peptides, 18 504 614 INDEX GnRH antagonist Gonadotropes (continued) desensitization, 161 LH and FSH types, 181 molecules crosslinked, 158 monohormonal and multihormonal, GnRH-like substances 188 produced by the gonads, 505 volume and area, 185 GnRH molecule Gonadotrope population binding affinity, 163 in adult female rat, 191 conformational analysis, 163 Gonadotrope responses GnRH occupancy requirements of, 163 receptor numbers, 161 Gonadotropic cell GnRH-R Golgi zone, 176 post-castration fall, 145 Gonadotrophic hormones GnRH recentor ovarian function, 200 down-regulation, 144 Gonadotroph microaggregation, 164 cAMP, 5 modulation of numbers, 208 cAMP, 5 in pituitary gonadotrophs, 210 control by peptides, 3 in suckling, 146 Gonadotrophs GnRH recep tors interaction with lactotrophs, correlation with GnRH secretion, 360 142 lactotroph junctional complexes, distribution, 132 355 identification, 129 many are monohormonal, 608 induce GnRH-R, 143 PRL-releasing activity, 362 physiological regulation, 142 signal to lactotrophs, 357 GnRH-receptor complex Gonadotroph-lactotroph intracellular compartments, 154 interactions, 362 GnRH-stimulated Gonadotropins gonadotropin release, 497 deglycosylated, 206 GnRH stimulation test deglycosylated derivatives, 235 anovulatory, amenorrheic women, removal of sugar residues, 223 565 resumption of meiosis, 325 GnRH target organs selective hypothalmic control, phospholipid labeling, 497 9-10 GnRH therapy strategies for inhibition, 220 in luteal phase deficiency, 571 Gonadotropin antagonists Golgi membrane antigen properties, 232 subcellular localization, 177 Gonadotropin function Golgi zone pulsatile administration of intracisternal transit of Gn-RH, 584 hormones, 170 Gonadotropin receptor Gonadectomy blocking agents, 221 increases synthesis of subunits, hormonal complex localization, 406 200 synthesis, release of gonado­ modified gonadotropin, 223 tropins, 400 Gonadotropin release Gonadotropes catecholamines, 10 in cell population, 184 Gonadotropin-releasing activity GnRH stimulated, 190 vertebrate LH-RHs, 445 INDEX 615 Gonadotropin secretion hCG antagonist acetylcholine, 13 termination of pregnancy, 231 cholecystokinin, 15 hCG and DG-hCG estrogen, 4 interstitial cells, 228 frequency modulated by LHRH, HDL and hCG binding 552 luteal cells, 299 neurotensin and gastrin, 15 HMC-CoA reductase how opiotes inhibit, 516 effect of estradiol and testos­ progesterone, 4 terone, 302 pulsatile pattern, 578 Hormone molecules role of the hypothalmaus, 584 gone astray, 73 substance P, 15 Hormone-receptor testosterone, 4 internalized, 204 VIP, 15 Hormone-receptor complexes Gonadotropin storage processing, 134 analysis of, 188 Hormonal regulation estrous cycle, 187 casein gene family, 392 Gonadotropin subunits Hormonal release mechanisms estradiol, 398, 400 multireceptor regulation, 56 synthesized as precursors, 406 Hormone storage Gonadotropin subunit precursor gonadotropes, 184 gonadectomy, 398 Hormones Gonadotropin synthesis in antral fluid, 312 effect of LHRH, 96 in follicle, 310 Granulosa Hormones in antral fluid steroid products, 471 functional correlates, 316 Granulosa cells Hybridization aromatase activity, 468 specific mRNAs, 404 cholesterol side-chain cleavage, Hypothalamic amenorrhea 470 gonadotropin secretion, 579 culture techniques, 249 grading, 580 OS morphological changes, 251 pituitary responses to Gn-RH, progesterone, 472 583 Granulosa cell differentiation pulsatle Gn-RH, 585 mechanism, 201 reduced Gn-RH, 584 Granulosa cell phosphodiesterase suprapituitary origin, 579 FSH regulation, 416 Hypothalamic factors Granulosa cell structure modulating prolactin, 367 degradation of hCG, 240 Hypothalamic granules GTP effect incubated with CuHistadine or binding somatostatin, 376 CuCysteine, 33 Hypothalamic implants into hpg mice, 148 hCG Hypothalamic mRNA arachidonic acid conversion, 349 translation products, 91, 93 luteal cell, 294 Hypothalamic-pituitary system in nuclear fractions, 204 main features, 606 ovarian secretion, 293 synthesis of T and E, 294 616 INDEX Insulin LH action in tetrahymena, 74 on prognenolone production, 426 Infertle women LH and FSH release clinical characteristics, 568 prolonged suppression, 523 results of GnRH therapy, 570 LH and GnRH Immunological probes increase PGE production, 503 membrane antigens, 178 LH and GnRHa
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