Acoustic Wave Biosensors for Biomechanical and Biological Characterization of Cells
ACOUSTIC WAVE BIOSENSORS FOR BIOMECHANICAL AND BIOLOGICAL CHARACTERIZATION OF CELLS by Huiyan Wu B.E., Xi’an Jiaotong University, P. R. China, 2008 M.E., Xi’an Jiaotong University, P. R. China, 2011 Submitted to the Graduate Faculty of Swanson School of Engineering in partial fulfillment of the requirements for the degree of Doctor of Philosophy University of Pittsburgh 2017 UNIVERSITY OF PITTSBURGH SWANSON SCHOOL OF ENGINEERING This dissertation was presented by Huiyan Wu It was defended on June 22, 2017 and approved by Dissertation Co-Advisor: James H.-C. Wang, Ph.D., Professor, Departments of Orthopaedic Surgery & Mechanical Engineering and Materials Science William S. Slaughter, Ph.D., Associate Professor, Department of Mechanical Engineering and Materials Science Patrick Smolinski, Ph.D., Associate Professor, Department of Mechanical Engineering and Materials Science Dissertation Advisor: Qing-Ming Wang, Ph.D., Professor, Department of Mechanical Engineering and Materials Science ii Copyright © by Huiyan Wu 2017 iii ACOUSTIC WAVE BIOSENSORS FOR BIOMECHANICAL AND BIOLOGICAL CHARACTERIZATION OF CELLS Huiyan Wu, PhD University of Pittsburgh, 2017 During past decades, interest in development of cell-based biosensors has increased considerably. In this study, two kinds of acoustic wave sensors are adopted as the cell-based biosensors to investigate the biomechanical and biological behaviors of cells, the quartz thickness shear mode (TSM) resonator and Love wave sensor. For the first part, the quartz TSM resonator is applied to detect the structural and mechanical properties of tendon stem/progenitor cells (TSCs), which are one kind of newly discovered adult cells in tendons, and the platelets from blood. Through the TSM resonator, the related viscoelastic properties of cells are extracted, which could indicate the state of cells in different physiological conditions.
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