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HSF1 Activation Cycle

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HSF1 Activation Cycle HSF1 Activation Cycle Inactive monomeric heat shock factor 1 (HSF1) exists predominantly in the cytoplasm and remains repressed by complex with regulatory proteins of HSF1 its own transcriptional targets of heat-shock protein (HSP) and cytosolic chaperonin TCP1 ring complex (TRiC). Upon a wide range of stress conditions, HSF1 is activated to undergo trimerization, nuclear translocation, and post-translational modifications (PTMs) including phosphorylation, acetylation, and sumoylation, converted into a DNA-binding-competent, active homotrimer. HSF1 is then modified by different inhibitory PTMs to cause DNA dissociation, HSF1 inactivation, and HSF1 recycling/degradation and maintained in a negative-feedback mechanism. HSP70 HSP40 HSP90 HSP90 HSP40 Inhibitory protein TRiC dissociation HSP70 TRiC Recycling Inhibitory chaperone re-association HSP70 HSP40 TRiC HSP90 Cofactor recruitment Gene DNA binding expression Phosphorylation Sumoylation HSF1 Activation Cycle HSF1 Transcription activation DNA dissociation Acetylation Phosphorylation Neef et al., 2011 Gomez-Pastor et al., 2017 HSF1 Catalog No. Products Name Applications Reactivity H00003297-M09 HSF1 monoclonal antibody (M09), clone 1A11 IF,S-ELISA,ELISA,WB-Re Human MAB16307 HSF1 monoclonal antibody, clone 10H8 WB,IP,ELISA,ICC,GSA,IHC-P Human H00003297-A01 HSF1 polyclonal antibody (A01) WB-Ce,ELISA,WB-Re Human PAB25360 HSF1 (phospho S307) polyclonal antibody WB-Ce,IF Human PAB25849 HSF1 (phospho S303) polyclonal antibody WB-Ce,IHC-P Human PAB29289 HSF1 (phospho T142) polyclonal antibody IHC-P Human,Mouse,Rat H00003297-P01 HSF1 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003297-R01 HSF1 Pre-design Chimera RNAi RNAi Human KA2770 HSF1 (Human) Cell-Based ELISA Kit Qual Human,Mouse HSP90 Catalog No. Products Name Applications Reactivity MAB2186 HSP90AA1 monoclonal antibody, clone 2G5.G3 IP,ELISA,ICC,IHC,WB-Ti Human,Mouse,Rat Abnova MAB6631 HSP90AA1/HSP90AA2 monoclonal antibody, WB,ELISA,IHC Human,Rat,S. pombe,Yeast clone Hyb-K41220A PAB10218 HSP90AA1 polyclonal antibody ELISA,IHC,WB-Tr Chicken,Fruit fly, Human, Monkey,Mouse,Rat Abnova Corporation 9F, No. 108, Jhouzih St., Neihu, Taipei 114, Taiwan Tel: + 886 2 8751 1888 Fax: + 886 2 6602 1218 www.abnova.com HSP90 Catalog No. Products Name Applications Reactivity P3387 HSP90AA1 (Human) Recombinant Protein SDS-PAGE MAB10686 HSP90AB1 monoclonal antibody, clone 5G4 ELISA,WB-Ce,IHC-P,IF,Flow Cyt Human,Monkey,Mouse,Rat PAB5725 HSP90AB1 (phospho S254) polyclonal antibody WB-Ce Human,Mouse,Rat PAB18096 HSP90AB1 polyclonal antibody IP,ELISA,WB-Ce,IHC-P,IF Human,Mouse,Rat H00003326-D01P HSP90AB1 purified MaxPab rabbit polyclonal antibody (D01P) WB-Ce,WB-Tr,PLA-Ce Human KA2778 HSP90AB1 (Human) Cell-Based ELISA Kit Qual Human,Mouse,Rat H00003326-P01 HSP90AB1 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003326-T02 HSP90AB1 293T Cell Transient Overexpression Lysate(Denatured) WB HSP70 Catalog No. Products Name Applications Reactivity MAB15248 HSP70 monoclonal antibody, clone 3G10 WB,WB-Ce,IHC-P,IF Human,Mouse,Pig MAB11390 HSPA1A monoclonal antibody, clone 5A5 IP,ICC,IHC,WB-Ti,IF Amphibia,Chicken,Fish,Fruit fly, Human,Mouse,Rat,Yeast H00003303-D01 HSPA1A MaxPab rabbit polyclonal antibody (D01) WB-Tr,IP Human H00003303-A01 HSPA1A polyclonal antibody (A01) WB-Ce,ELISA,WB-Re Human H00003303-B01P HSPA1A purified MaxPab mouse polyclonal antibody (B01P) WB-Tr Human P3391 HSPA1A (Human) Recombinant Protein SDS-PAGE H00003304-M02 HSPA1B monoclonal antibody (M02), clone 3B7 WB-Ce,IHC-P,IF,S-ELISA,ELISA, Human,Mouse,Rat WB-Re,PLA-Ce H00003304-A01 HSPA1B polyclonal antibody (A01) WB-Ce,ELISA,WB-Re Human H00003304-Q01 HSPA1B (Human) Recombinant Protein (Q01) AP,Array,ELISA,WB-Re H00003304-R01 HSPA1B Pre-design Chimera RNAi RNAi Human H00003305-M02 HSPA1L monoclonal antibody (M02), clone 7H6 WB-Ce,IHC-P,S-ELISA,ELISA, Human,Rat WB-Re,PLA-Ce H00003305-D01P HSPA1L purified MaxPab rabbit polyclonal antibody (D01P) WB-Ti,WB-Tr,PLA-Ce Human,Mouse H00003305-D01 HSPA1L MaxPab rabbit polyclonal antibody (D01) WB-Ti,WB-Tr,IP Human,Mouse H00003305-A01 HSPA1L polyclonal antibody (A01) WB-Ce,ELISA,WB-Re Human H00003305-R01 HSPA1L Pre-design Chimera RNAi RNAi Human H00003305-PW2 HSPA1L (Human) IP-WB Antibody Pair IP-WB Human H00003305-AP21 HSPA1L (Human) Matched Antibody Pair EPair-Re Human H00003305-P01 HSPA1L (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003305-T01 HSPA1L 293T Cell Transient Overexpression Lysate(Denatured) WB H00003306-M06 HSPA2 monoclonal antibody (M06), clone S51 WB-Ce,IHC-P,S-ELISA,ELISA, Human WB-Re,IP H00003306-B01P HSPA2 purified MaxPab mouse polyclonal antibody (B01P) WB-Ce,IF,WB-Tr Human H00003306-PW2 HSPA2 (Human) IP-WB Antibody Pair IP-WB Human H00003306-P01 HSPA2 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003306-T01 HSPA2 293T Cell Transient Overexpression Lysate(Denatured) WB H00003308-M01 HSPA4 monoclonal antibody (M01), clone 3A11 S-ELISA,ELISA,WB-Re,WB-Tr Human MAB3737 HSPA4 monoclonal antibody, clone W27 IP,IHC-P,IF,Flow Cyt Bovine,Human,Monkey MAB6130 HSPA4/HSPA8 monoclonal antibody, clone N27F3-4 WB,IP,IHC,Flow Cyt Bovine,Chicken,Dog,Fish,Frog,Fruit fly, Guinea pig,Hamster,Human,Monkey, Mouse,Nematoda,Pig,Plants,Rab H00003308-D01 HSPA4 MaxPab rabbit polyclonal antibody (D01) WB-Ce,WB-Ti,WB-Tr,IP Human,Mouse H00003308-D01P HSPA4 purified MaxPab rabbit polyclonal antibody (D01P) WB-Ce,WB-Ti,WB-Tr Human,Mouse PAB30574 HSPA4 polyclonal antibody WB,WB-Ce,IHC-P,IF Human,Mouse,Rat H00003308-PW1 HSPA4 (Human) IP-WB Antibody Pair IP-WB Human H00003308-P01 HSPA4 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003308-T04 HSPA4 293T Cell Transient Overexpression Lysate(Denatured) WB H00003312-M01 HSPA8 monoclonal antibody (M01), clone 1G2 S-ELISA,ELISA,WB-Re Human MAB6636 HSPA8 monoclonal antibody, clone 1F2-H5 WB,IP,ELISA,IHC Human,Mouse,Rat PAB15412 HSPA8 polyclonal antibody WB,IP,IHC,IF,Blocking Hamster,Human PAB8771 HSPA8 polyclonal antibody WB,IHC Bovine,Hamster,Human,Monkey, Mouse,Pig,Rat,Sheep H00003312-R01 HSPA8 Pre-design Chimera RNAi RNAi Human H00003312-P01 HSPA8 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re Abnova Corporation 9F, No. 108, Jhouzih St., Neihu, Taipei 114, Taiwan www.abnova.com Tel: + 886 2 8751 1888 Fax: + 886 2 6602 1218 HSP40 Catalog No. Products Name Applications Reactivity H00003301-M03 DNAJA1 monoclonal antibody (M03), clone 4F2 ELISA Human MAB3738 DNAJA1 monoclonal antibody, clone KA2A5.6 WB,IP,IHC-Fr,IHC-P,IF,Flow Cyt Bacteria,Hamster,Human,Monkey, Mouse,Pig,Rabbit,Rat PAB29361 DNAJA1 polyclonal antibody WB-Ce,IHC-P,IF Human,Mouse,Rat H00003301-R01 DNAJA1 Pre-design Chimera RNAi RNAi Human H00003301-P01 DNAJA1 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00010294-M01 DNAJA2 monoclonal antibody (M01), clone 2A11-F2 WB-Ce,IF,S-ELISA,ELISA,WB-Re Human H00010294-A01 DNAJA2 polyclonal antibody (A01) ELISA,WB-Re Human H00010294-R01 DNAJA2 Pre-design Chimera RNAi RNAi Human H00010294-P01 DNAJA2 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00009093-B01 DNAJA3 MaxPab mouse polyclonal antibody (B01) IF,WB-Tr Human H00009093-B01P DNAJA3 purified MaxPab mouse polyclonal antibody (B01P) IF,WB-Tr Human H00009093-R01 DNAJA3 Pre-design Chimera RNAi RNAi Human H00009093-P01 DNAJA3 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00009093-T01 DNAJA3 293T Cell Transient Overexpression Lysate(Denatured) WB H00055466-M01 DNAJA4 monoclonal antibody (M01), clone 4B4-1F2 WB-Ce,IHC-P,IF,S-ELISA, Human ELISA,WB-Re H00055466-A01 DNAJA4 polyclonal antibody (A01) ELISA,WB-Re Human H00055466-R01 DNAJA4 Pre-design Chimera RNAi RNAi Human H00055466-B01P DNAJA4 purified MaxPab mouse polyclonal antibody (B01P) WB-Tr Human H00055466-B01 DNAJA4 MaxPab mouse polyclonal antibody (B01) WB-Tr Human H00055466-P01 DNAJA4 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00055466-T01 DNAJA4 293T Cell Transient Overexpression Lysate(Denatured) WB MAB2185 DNAJB1 monoclonal antibody, clone 3B9.E6 WB,IP,ELISA,IHC Human,Mouse,Rat H00003337-B01 DNAJB1 MaxPab mouse polyclonal antibody (B01) WB-Ti,WB-Tr Human H00003337-D01 DNAJB1 MaxPab rabbit polyclonal antibody (D01) IP Human PAB15411 DNAJB1 polyclonal antibody IP,WB-Ce Human KA2775 DNAJB1 (Human) Cell-Based ELISA Kit Qual Human,Mouse,Rat H00003337-P01 DNAJB1 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003337-T01 DNAJB1 293T Cell Transient Overexpression Lysate(Denatured) WB H00003300-M03 DNAJB2 monoclonal antibody (M03), clone 2C9 WB-Ce,IHC-P,IF,S-ELISA, Human ELISA,WB-Re H00003300-D01P DNAJB2 purified MaxPab rabbit polyclonal antibody (D01P) WB-Tr Human H00003300-D01 DNAJB2 MaxPab rabbit polyclonal antibody (D01) WB-Tr,IP Human H00003300-A01 DNAJB2 polyclonal antibody (A01) ELISA,WB-Re Human H00003300-R01 DNAJB2 Pre-design Chimera RNAi RNAi Human H00003300-P01 DNAJB2 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00003300-T01 DNAJB2 293T Cell Transient Overexpression Lysate(Denatured) WB H00414061-B01P DNAJB3 purified MaxPab mouse polyclonal antibody (B01P) WB-Tr Human H00011080-M01 DNAJB4 monoclonal antibody (M01), clone 1H2-3G4 WB-Ce,S-ELISA,ELISA,WB-Re, Human WB-Tr H00011080-B01 DNAJB4 MaxPab mouse polyclonal antibody (B01) WB-Ti,WB-Tr Human H00011080-A01 DNAJB4 polyclonal antibody (A01) ELISA,WB-Re Human H00011080-R01 DNAJB4 Pre-design Chimera RNAi RNAi Human H00011080-B01P DNAJB4 purified MaxPab mouse polyclonal antibody (B01P) WB-Ti,WB-Tr Human H00011080-P01 DNAJB4 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00011080-T02 DNAJB4 293T Cell Transient Overexpression Lysate(Denatured) WB L072T6 DNAJB4 HEK293 Cell Transient Overexpression Lysate WB,IP (Non-Denatured) H00025822-M01 DNAJB5 monoclonal antibody (M01), clone 1C3 WB-Ce,S-ELISA,ELISA,WB-Re Human H00025822-R01 DNAJB5 Pre-design Chimera RNAi RNAi Human H00025822-P01 DNAJB5 (Human) Recombinant Protein (P01) AP,Array,ELISA,WB-Re H00010049-M01 DNAJB6 monoclonal antibody (M01), clone 2C11-C1 WB-Ce,S-ELISA,ELISA, Human WB-Re,WB-Tr Abnova Corporation 9F, No.
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