Identification of Scavenger Receptor B1 As the Airway Microfold Cell
RESEARCH ARTICLE Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis Haaris S Khan1, Vidhya R Nair1, Cody R Ruhl1, Samuel Alvarez-Arguedas1, Jorge L Galvan Rendiz1, Luis H Franco1†, Linzhang Huang2, Philip W Shaul2, Jiwoong Kim3, Yang Xie3,4,5, Ron B Mitchell6, Michael U Shiloh1,7* 1Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States; 2Center for Pulmonary and Vascular Biology, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, United States; 3Quantitative Biomedical Research Center, Department of Population and Data Sciences, University of Texas Southwestern Medical Center, Dallas, United States; 4Harold C Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, United States; 5Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, United States; 6Department of Otolaryngology, University of Texas Southwestern Medical Center, Dallas, United States; 7Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, United States Abstract Mycobacterium tuberculosis (Mtb) can enter the body through multiple routes, *For correspondence: including via specialized transcytotic cells called microfold cells (M cell). However, the mechanistic michael.shiloh@utsouthwestern. basis for M cell entry remains undefined. Here, we show that M cell transcytosis depends on the edu Mtb Type VII secretion machine and its major virulence factor EsxA. We identify scavenger Present address: †Federal receptor B1 (SR-B1) as an EsxA receptor on airway M cells. SR-B1 is required for Mtb binding to University of Minas Gerais, Belo and translocation across M cells in mouse and human tissue. Together, our data demonstrate a Horizonte, Brazil previously undescribed role for Mtb EsxA in mucosal invasion and identify SR-B1 as the airway M Competing interests: The cell receptor for Mtb.
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