CSIRO PUBLISHING Marine and Freshwater Research http://dx.doi.org/10.1071/MF16006 Genetic analyses reveal limited dispersal and recovery potential in the large freshwater crayfish Euastacus armatus from the southern Murray–Darling Basin Nick S. WhiterodA,E, Sylvia ZukowskiA, Martin AsmusB, Dean GilliganC and Adam D. MillerA,D,E AAquasave – Nature Glenelg Trust, Goolwa Beach, SA 5214, Australia. BNSW Department of Primary Industries, Narrandera, NSW 2700, Australia. CNSW Department of Primary Industries, Batemans Bay, NSW 2536, Australia. DSchool of Life & Environmental Sciences, Centre for Integrative Ecology, Deakin University, Warrnambool, Vic. 3280, Australia. ECorresponding authors. Email:
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[email protected] Abstract. Understanding dispersal traits and adaptive potential is critically important when assessing the vulnerability of freshwater species in highly modified ecosystems. The present study investigates the population genetic structure of the Murray crayfish Euastacus armatus in the southern Murray–Darling Basin. This species has suffered significant population declines in sections of the Murray River in recent years, prompting the need for information on natural recruitment processes to help guide conservation. We assessed allele frequencies from 10 polymorphic microsatellite loci across 20 sites encompassing the majority of the species’ range. Low levels of gene flow were observed throughout hydrologically connected waterways, but significant spatial autocorrelation and low migration rate estimates reflect local genetic structuring and dispersal limitations, with home ranges limited to distances ,50-km. Significant genetic differentiation of headwater populations upstream of barriers imposed by impoundments were also observed; however, population simulations demonstrate that these patterns likely reflect historical limitations to gene flow rather than contemporary anthropogenic impacts.