Surgical Anatomy and Histology of the Levator Palpebrae Superioris Muscle
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View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Directory of Open Access Journals Strana 1124 VOJNOSANITETSKI PREGLED Vojnosanit Pregl 2013; 70(12): 1124–1131. UDC: 611.847::617.77-089 ORIGINAL ARTICLE DOI: 10.2298/VSP1312124D Surgical anatomy and histology of the levator palpebrae superioris muscle for blepharoptosis correction Hirurška anatomija i histologija mišiüa podizaþa gornjeg kapka u korekciji blefaroptoze Boban Djordjeviü*, Marijan Novakoviü†‡, Milan Milisavljeviü§, Saša Miliüeviü*, Aleksandar Malikoviü§ *Clinic for Plastic Surgery and Burns, ‡Head Office, Military Medical Academy, Belgrade, Serbia; †Faculty of Medicine of the Military Medical Academy, University of Defense, Belgrade, Serbia; §Institute of Anatomy, Faculty of Medicine, University of Belgrade, Belgrade, Serbia Abstract 1.41 mm on the right, and 40.3 ± 1.63 mm on the left side. In all the cases, the LPS had blood supply from 4 different Background/Aim. The detailed knowledge of the archi- arterial systems: the lacrimal, supratrochlear, and supraorbital tecture of the upper eyelid is very important in numerous artery and muscle branches of the ophthalmic artery. The upper eyelid corrective surgeries. The article deals with the LPS muscle in all the specimens was supplied by the superior detailed anatomy of the major components of the upper lid, medial branch of the oculomotor nerve. The connective tis- which are commonly seen in surgical practice. Methods. sue associated with the LPS muscle contains two transverse This study was conducted on 19 human cadavers (12 adults ligaments: the superior (Whitnall’s) and intermuscular trans- and 7 infants) without pathologic changes in the orbital re- verse ligaments (ITL). The orbital septum in all the speci- gion and eyelids. Anatomic microdissection of the contents mens originated from the arcus marginalis of the frontal of the orbita was performed bilaterally on 12 orbits from 6 bone, and consisted of two layers – the superficial and the unfixed cadavers (3 male and 3 female). Micromorphologic inner layer. In addition, a detailed histological analysis re- investigations of the orbital tissue were performed on 8 en vealed that the upper eyelid’s crease was formed by the con- bloc excised and formalin-fixed orbits of infant cadavers. joined fascia including the fascia of the orbicularis muscle, Specimens were fixed according to the Duvernoy method. the superficial layer of the orbital septum, and the aponeuro- An intra-arterial injection of 5% mixture of melt formalin sis of the LPS muscle, as well as the pretarsal fascia. Conclu- and black ink was administered into the carotid arterial sys- sion. The conducted study provided a valuable morphologi- tem. Using routine fixation, decalcination, dehydration, illu- cal basis for biomechanical and clinical considerations re- mination, impregnation and molding procedures in paraplast, garding blepharoptosis surgery. specimens were prepared for cross-sections. Results. The measurement of the muscle length and diameter in situ in 6 Key words: nonfixed cadavers (12 orbits) showed an average length of oculomotor muscles; blepharoptosis; microdissection; the levator palpbrae superioris (LPS) muscle body of the 42.0 ± oculomotor nerve. Apstrakt orbita kadavera odojÿadi fiksiranih formalinom. Preparati su fiksirani Duvernoy metodom. U karotidni sistem intraarte- Uvod/Cilj. Detaljno poznavanje graĀe gornjeg kapka veo- rijski je ubrizgavana mešavina 5% rastvora formalina i crnog ma je važno za mnogobrojne korektivne hirurške zahvate na tuša. Rutinskom procedurom, koja obuhvata fiksaciju, de- gornjem kapku. Ovaj ÿlanak bavi se detaljnom anatomijom kalcifikaciju, dehidrataciju, prosvetljavanje, impregnaciju i glavnih struktura gornjeg kapka koji se obiÿno susreýu u hi- kalupljenje u paraplastu, uzorci su pripremani za pravljenje rurškoj praksi. Metode. Studija je sprovedena na 19 ljudskih preseka. Rezultati. Merenje dužine i širine mišiýa na šest kadavera (12 odraslih i 7 odojÿadi) bez patoloških promena svežih kadavera (12 orbita) pokazalo je proseÿnu dužinu tela u orbitalnoj regiji i kapcima. Anatomska mikrodisekcija sa- mišiýa levator palpebrae superioris (LPS) od 42,0 ± 1,41 mm na držaja orbite sprovedena je na 12 orbita, obostrano na 6 desnoj strani, a 40,3 ± 1,63 mm na levoj strani. U svim slu- svežih kadavera odraslih (3 muška i 3 ženska). Mikromor- ÿajevima, LPS je bio vaskularizovan iz ÿetiri razliÿita arterij- fološka ispitivanja struktura orbite izvedena su na 8 en bloc ska sistema: a. lacrimalis, a. supratrochlearis, a. supraorbitalis i mi- Correspondence to: Boban Djordjeviý, Milovana Šaranoviýa 14, 11 000 Belgrade, Serbia. Phone: +381 64 2 096 096. E-mail: [email protected] Volumen 70, Broj 12 VOJNOSANITETSKI PREGLED Strana 1125 šiýnih grana a. ophthalmicae. Mišiý LPS u svim sluÿajevima bicularis oculi, površni sloj orbitalnog septuma, aponeuroza inervisala je gornja medijalna grana n. oculomotoriusa. Usta- mišiýa levator palpebrae superioris i pretarzalna fascija. Zak- novljeno je da je vezivno tkivo povezano sa LPS mišiýem, ljuÿak. Istraživanjem je obezbeĀena važna morfološka os- sastavljeno od dva popreÿna ligamenta: gornjeg (Whit- nova za biomehaniÿka i kliniÿka ispitivanja u hirurgiji ble- nallovog) i intermuskularnog popreÿnog ligamenta (ITL). faroptoza. Orbitalni septum je u svim sluÿajevima polazio od supra- orbitalne ivice ÿeone kosti i sastojao se od dva sloja – po- Kljuÿne reÿi: vršnog i dubokog. Osim toga, detaljna histološka analiza mišiýi, okulomotorni; blefaroptoza; mikrodisekcija; pokazala je da gornji kapaÿni žleb formira fascija mišiýa or- n. oculomotorius. Introduction muscle was dissected to expose the tarsal plate. Microdis- section of the orbital subject was performed using a stereo A wider knowledge of the levator palpebrae superioris magnifying glass (4u). (LPS) muscle and the suspensory fibrous tissue related to the The method of standard dissection of 12 orbits from 6 LPS muscle is essential for the eyelid surgery, especially for formalin fixed cadavers (3 male and 3 female) was per- the blepharoptosis correction. Even though, there are several formed by the combined transcutaneous and transcranial ap- papers providing the description of the eyelid anatomy, anat- proach. Incisions were made on the skin, the orbicularis oculi omic features of the orbicularis oculi and the LPS muscle, muscle was exposed, and a strip of orbicularis oculi muscle there are various anatomical details that are still controver- was turned aside. After the junction of the orbital septum and sial. In particular, the importance of the orbital connective levator aponeurosis was observed, a blunt dissection was car- tissue and the role they play in pathogenesis or the blepha- ried out through the outer layer of the orbital septum, and roptosis treatment are still unclear. continued to the Whitnall’s ligament between the levator In most patients with congenital and acquired blepha- aponeurosis and the inner layer of the orbital septum, which roptosis, palpebral creases are not so distinctive. In addition, covers the posterior surface of the orbital fat. Anderson et al. 1 described an atrophic and dehiscent supe- All the specimens were photographed by a Sony Cyber rior transverse ligament (Whitnall’s ligament-WL 2) in those Shot DCS P8 digital camera. patients. Fink et al. 3 first reported the presence of the con- Micromorphologic investigations of the orbital tissue nective tissue which underlines the LPS muscle, the tissue were performed on 8 en bloc excised and formalin-fixed orbits that was called the intermuscular transverse ligament (ITL) of infant cadavers with no pathologic changes in the orbital re- by Lukas et al. 4. However, the functional role of these liga- gion and eyelids. An intra-arterial injection of 5% mixture of ments in the LPS muscle is still unclear. melt formalin and black ink was administered in the carotid The aim of this study was to reinvestigate the detailed arterial system, and rinsed out using a saline solution and 4% anatomy of the connective tissues related to the LPS muscle, neutral buffered solution of formaldehyde. The specimens and to establish their role in the suspension and creation of were fixed according to the method of Duvernoy. We used the normal upper eyelid contours in the occidental race. standard technique for the brain removal, and the specific tran- scranial approach. Microdissections (using micro instruments) Methods of injected orbital blood vessels, nerves and muscles were analyzed under the Leica MZ6 stereomicroscope. The anatomic microdissection study and histological All the specimens were photographed by Sony Cyber analysis were conducted on 19 human cadavers (12 adults Shot DCS P8 digital camera. To get more easily visible de- and 7 infants). We used microdissection techniques for the tails, we used the Leica DC 300 digital camera (magnifica- orbits from formalin-unfixed/fixed cadavers, as well as mi- tions 6.3u, 10u and 20u). cromorphologic investigation methods. Histological analysis was carried out on 6 en bloc ex- The anatomic microdissection of the contents of the or- cised and formalin-fixed infant orbits. We used the material bita through the anterior cranial fossa (transcranial approach) from the Anatomic Institute of the Faculty of Medicine, Bel- was performed bilaterally on 12 orbits from six unfixed ca- grade. The aim was to establish the exact micromorphologic davers (3 male and 3 female). The cadavers, aged 42–75 architecture of the upper