AIBNAustralian Institute for Bioengineering and Nanotechnology Annual Report 2015 Scanning electron microscopy samples undergoing preparation at the Centre for Microscopy and Microanalysis facility at AIBN.

2 - AIBN Annual Report 2015 AIBN Annual Report 2015

Vice-Chancellor and President’s Message 2 Discoveries and collaborations 28 MERS antibody building on Hendra virus work 30 Director’s Message 3 Cheaper, cleaner production of carbon fibre 31 AIBN Board 4 Getting biomarkers out of the skin Scientific Advisory Committee 5 with Micropatches 32 AIBN Research 6 High performance cluster boosts Alexandrov Group research capabilities 33 Synthetic biology drives diagnostic care 8 Self-assembled nanocapsules deliver results 34 Bernhardt Group Using theoretical and computational science Fast, cheap and easy to use for nanomaterials and fluids 9 tuberculosis screening 35 Cooper-White Group IAP removes barriers between academia Biomaterials development and discovery for and industry 36 regenerative medicine 10 AIBN Early-/Mid-Career Support Program 37 Gray Group Funding and recognition 38 Mammalian cell expression and bulk stem cell Australian Research Council funding 40 cultivation 11 National Health and Medical Research Council 41 Halley Group Biopolymers and starch for a Fellowships fuel research opportunities 42 cheaper, cleaner future 12 Awards highlight scientific excellence 43 Kendall Group Newly awarded research funding Gene and drug delivery and diagnostics commencing in 2015 44 through the skin 13 Facilities and infrastructure 46 Mahler Group Infrastructure facilitates research 48 Biologics for medical applications 14 Students and graduates 50 Martin Group Student difference 52 Advances make plastic truly fantastic 15 AIBN Student Association 53 Middelberg Group Self-assembling soft condensed Student highlights 54 materials science 16 Research Higher Degree students 56 Monteiro Group 2015 graduate list 58 Synthesis and application of complex polymer architectures 17 Milestone student develops career 64 Neilsen Group High achieving graduate takes on Systems biology to unlock biological US challenge 65 complexity 18 Engagement 66 Trau Group Scientific engagement expands AIBN’s reach 68 Point-of-care diagnostics to save lives 19 Community and school engagement activities 70 Wang Group AIBN Seminar Series 71 Development of solar conversion and storage Publications 72 materials for a greener future 20 Publications 74 Wells Group Contact AIBN 85 Big data shows big stem cell possibilities 21 Whittaker Group Innovative polymer chemistry for health and IT 22 Wolvetang Group Functional genomics drive regenerative medicine 23 Xu Group Nanoparticles to boost healthcare 24 Yu Group Applied functional materials 25 Cover image: A high density microfluidic array Associate Group Leaders 26 for rapid screening of cells under development in the Cooper-White laboratory.

AIBN Annual Report 2015 - 1 Vice-Chancellor and President’s Message

With its proven record for propelling the leading four of them. The industry-supported Just as young, exciting researchers begin riding outcomes of excellent research from the grants are worth more than $1.7 million in total. the innovation escalator, so the seasoned laboratory to end-users, AIBN is primed to be a veterans make way for others at the top. At heavy-lifter for national innovation and global An exciting example of downstream potential the year’s end, Professor Peter Gray retired problem-solving. is exemplified by the Nanopatch, an original as Inaugural Director of AIBN, having built technology developed in the AIBN laboratory it into an institute of international standing, of Professor Mark Kendall. It is one of UQ’s respected by collaborators from leading global After more than a decade of hot innovation prospects. In 2015 Vaxxas, the institutions, as well as leaders in business, increasingly robust associations start-up that has grown around the invention, government and philanthropy. attracted $25 million in series B financing to with industry, 2015 saw the bring its total capital raising to $40 million. I look forward to working with Peter’s Institute and its partners The new funding will further the novel successor, Professor Alan Rowan, an vaccine delivery device‘s progress towards outstanding scientist and energetic leader continue to demonstrate the clinical trials. Vaxxas has previously forged a who has joined UQ from the Institute of benefits and exciting potential partnership with pharmaceutical giant Merck Molecules and Materials at Radboud University & Co. Nijmegen, a highly-regarded European centre of partnerships between strong for nanoscience research and training. With researchers and innovative This annual report highlights many other an impressive background in molecular notable 2015 developments. Among them are systems, Alan is ideally placed to lead AIBN to companies. Professor Lars Nielsen’s success in securing its next stage of excellence and impact, where an $8.6 million Laureate Research Grant from outstanding research and partnerships will The AIBN again showed that it has both raw the Novo Nordisk Foundation, and Associate continue to create advantageous change. research power, and the capacity to translate Professor Zhi Ping “Gordon” Xu’s receipt of this into great outcomes for society. The raw $100,000 of philanthropic funding from China. power is reflected in some of The University In both cases, the funding supports work that Effective leaders need focussed of Queensland’s strongest scores in the strives to benefit cancer patients. Excellence in Research for Australia evaluation and energetic teams, and I am (published in December). AIBN contributed Crucially, AIBN staff do not only pursue their confident that is what Alan will to six fields where UQ gained the highest own research goals; they also impart their possible rating of ‘well above world standard’ find at AIBN. wisdom and experience to students. The – including one field, industrial biotechnology, resulting talent cycle is essential for national In closing, I would like to thank and where UQ is the outright national leader. and global productivity and prosperity. It was congratulate Peter and all AIBN staff, students, therefore very satisfying to see AIBN pass the partners and supporters for another strong Industry’s appreciation of the downstream milestone of 100 PhD conferrals in 2015. The year, which will be the springboard to a great potential of this research is evident in AIBN’s one hundredth graduate, Dr Nick Fletcher, is future. success in the vigorous contest for Australian highlighted in these pages. Research Council Linkage Project grants. AIBN Professor Peter Høj is involved in six new Linkage-funded projects, Vice-Chancellor and President

2 - AIBN Annual Report 2015 Director’s Message

We live in exciting times as our ability to look an exceptional career Professor John Drennan The support of the greater University of down to and manipulate at the atomic scale retires, leaving the Centre for Microscopy and Queensland also deserves our thanks. Since allows for tantalising prospects. New scientific Microanalysis as his AIBN legacy. Professor my arrival to this university I have been discoveries are made every day, and AIBN has Peter Halley’s dedication saw him promoted pleased to see the collaborative nature UQ proven to be one of the world leaders in turning to Head of School of Chemical Engineering at shares across its institutes, schools, centres these discoveries into applications for better UQ. Furthermore, Professor Ajayan Vinu took and faculties, which I intend to further health and sustainable solutions for energy, up a Professorship at the University of South explore. The leadership of Professor Peter manufacturing and more. Many technologies Australia. We wish them all well. Høj (Vice-Chancellor and President), together developed under AIBN’s roof play a significant with Professor Max Lu (Provost), Professor role in shaping our society and environment for These departures bring renewed opportunity; Robyn Ward (Vice-Chancellor (Research)) the better. for individuals to rise to the challenge of and Professor Anton Middelburg (Pro-Vice- scientific endeavour, and for new ideas to Chancellor (Research and International)) has In 2015 with the stepping down of Inaugural flourish. Coming to AIBN with my external been pivotal in the success of the university in Director Professor Peter Gray, AIBN saw the perspective from the Radboud University recent years, and fostering an environment in end of an era that started with its foundation Nijmegen, Netherlands, in my opinion the which AIBN has been able to thrive. in 2002. Institute’s greatest strengths are its dedication to applied research outcomes and the breadth I look forward to a future in which AIBN will and scope of the work undertaken. They are continue to turn ground breaking science I now have the honour to further a truly unique and remarkable combination. I into innovative applications, from advanced develop this Institute with its look forward to cultivating those strengths and materials and diagnostic tools, to cures for building upon them. important diseases and many more. proven reputation for research excellence, its world class Within the pages of this report you will find Close cooperation with medical facilities, and its talented cohort summaries of the work, achievements and success stories accomplished at AIBN during professionals and industries of more than 450 staff and 2015. Reading it myself, I am enthused by the will be key to achieving such students. high quality of the work being accomplished. The year saw the Institute enjoy success in goals, as is providing promising science, as well as in funding, awards and The Institute owes tremendous gratitude recognition. The achievements highlighted here young academics with the to Professor Peter Gray for his courage and are a credit to the researchers, students and dedication to join UQ and establish this entrepreneurial skills required support staff that make up this Institute. incredible lasting legacy. I am excited to step to turn cutting edge science into this role with the foundations well and truly established; the people in place, the research into successful products. The I would like to particularly recognise the work programs developed and infrastructure built. of the AIBN Board, which has been ably chaired foundations for a dynamic There are a great – and ever increasing – by Euan Murdoch, and fellow Board members number of people who owe their careers and AIBN, recognised around the Kathy Hirschfeld, Chris Lowe, Bob McCarthy, livelihoods to AIBN, and I hope Professor Susan Pond and Robyn Ward. Additionally, world, have been laid. Now it is Gray takes great satisfaction in what he has I would like to acknowledge the efforts and achieved. my challenge to enable it. direction provided by the Scientific Advisory Board. Professor Alan Rowan In 2015 the Institute saw further change, with AIBN Director two foundation Group Leaders departing. After

AIBN Annual Report 2015 - 3 AIBN Board

Following its establishment in 2011, AIBN has drawn on the interdisciplinary expertise of the Board whose collective experience spans industrial, corporate, research, government and academic sectors.

Together with the Scientific Advisory Committee, the AIBN Board plays a key role in the development and strategic planning of the Institute.

2015 Board membership Euan Murdoch (Chair) Dr Susan Pond AM Founder of Australian-owned Herron Adjunct Professor in The Dow Sustainability Pharmaceuticals, Mr Euan Murdoch has held Program at the United States Study Centre at the Board responsibilities positions including on the Australian Food University of Sydney, Dr Susan Pond has a strong  and Grocery Council, the Complementary scientific and commercial background. She has Assist in defining strategic goals and Healthcare Council of Australia, the Queensland held executive positions in the biotechnology and progress against goals  Biotechnology Advisory Council, the Reserve pharmaceutical industry for 12 years, including Assist in defining levels of funding Bank of Australia Small Business Advisory as Chair and Managing Director of Johnson & required to support ongoing Board, Harvest Fresh Cuts Pty Ltd and Johnson Research Pty Ltd. Her board positions operations and strategic initiatives  Sigma Pharmaceuticals. In 2000, he received include the Australian Nuclear Science and Provide advice on funding the Bicentenary Medal for services to the Technology Organisation, Commercialisation opportunities, commercialisation pharmaceutical and complementary healthcare Australia, the Academy of Technological paths, extension activities and growth industry. Sciences and Engineering (as Vice-President), strategies for the Institute  Biotron Ltd and the Centenary Institute. Review progress of the Institute in the areas of Professor Peter Gray research, internationalisation, Inaugural Director of AIBN, 2003-2015. Professor Robyn Ward AM commercialisation, governance and Professor Peter Gray has held positions at Deputy Vice Chancellor (Research) at UQ, management University of New South Wales as Director of Professor Robyn Ward has held roles including Provide advice on matters such as the Bioengineering Centre and Professor of Director of the Prince of Wales Cancer Centre, raising the international profile of the Biotechnology, and at the Garvan Institute of and Clinical Associate Dean at University of Institute to maximise the benefits to Medical Research as a Senior Principal Research New South Wales. She remains Director of the Queensland and Australia Fellow. Professor Gray has had commercial Translational Cancer Research Network, a multi- Assisting to maintain the high experience in the US, working for Eli Lilly and Co institutional group based in NSW and supported visibility and reputation of AIBN in and Cetus Corporation. He has held academic by the Cancer Institute NSW, and chairs the research, industry, government and positions at the University College of London and Medical Services Advisory Committee, and is a public domains. the University of California, Berkeley. long standing member of the Pharmaceutical Benefits Advisory Committee. Kathy Hirschfeld Non-executive director of InterOil Corp, Transfield Services Limited, and Toxfree Solutions; a Senator of The University of Queensland; and a member of the Board of UN Women in Australia. A chemical engineer, Ms Kathy Hirschfeld’s 20- year career with BP included oil refining, logistics and exploration, located in Australia, the UK and Turkey. Her last executive role was as Managing Director of BP Bulwer Island Refinery in Brisbane, with responsibility for all aspects of the business.

Emeritus Professor Chris Lowe OBE Affiliated with the Institute of Biotechnology at the Department of Chemical Engineering and Biotechnology at the University of Cambridge, Emeritus Professor Chris Lowe is a fellow of the Royal Academy of Engineering, the Institute of Physics and the Royal Society of Chemistry. He has been the driving force for the establishment of 11 spin-out companies and fosters entrepreneurship within the University. Professor Lowe is also Chair of the AIBN Scientific Advisory Committee.

Bob McCarthy AM With more than three decades of experience in senior positions in both the public and private sectors, Mr Bob McCarthy has been Director General of several Queensland Government departments, including the Department of Natural Resources and Mines, and the Department of State Development AIBN Board Members (from left to right): Bob McCarthy AM, Professor Peter Gray, Kathy Hirshfeld, and Innovation. He has been at the forefront of Emeritus Professor Chris Lowe OBE, Dr Susan Pond, Euan Murdoch, Professor Robyn Ward AM, efforts to diversify the Queensland economy and Dr Zoe Cahill. develop new industries, based on science and innovation.

4 - AIBN Annual Report 2015 Scientific Advisory Committee

The Scientific Advisory Committee is comprised of renowned researchers from across the globe. The group are tasked with advising the AIBN Board and Director on scientific direction and research strategies.

This highly skilled group are leaders in their fields, and are at the forefront of scientific research. Their combined expertise and knowledge is a valuable source for AIBN to use for maintaining its leadership position. 2015 Committee membership Professor Chris Lowe (Chair) Dr Anita Hill Professor Virgil Percec Professor of Biotechnology, University of Chief of Process Science and Engineering, P Roy Vagelos Chair and Professor of Cambridge CSIRO Chemistry, University of Pennsylvania

Professor Chunli Bai Professor Andrew Holmes Professor Colin Raston President, Chinese Academy of Sciences University Laureate Professor of Chemistry, South Australian Premier’s Professorial CSIRO Fellow and Distinguished Research Research Fellow in Clean Technology, Flinders Professor Harvey W Blanch Fellow (Imperial), University of Melbourne University The Merck Professor of Biochemical Engineering, University of California, Berkeley Professor Martin Lavin Professor Laura Poole-Warren Centre for Clinical Research, The University of Pro Vice-Chancellor Research Training and Professor Barry Buckland Queensland Dean Graduate Research School, University of Visiting Professor Department of Biochemical New South Wales Engineering, Faculty of Engineering Science, Professor Martin Pera University College London; CEO of BioLogicB Chair of Stem Cell Sciences, University of LLC Melbourne, Florey Neuroscience and Mental Health Institute and Walter and Eliza Hall Professor Thomas W Healy AO Institute of Medical Research Particulate Fluids Processing Centre, University of Melbourne

Committee responsibilities

Identify future strategic opportunities for fields of research, collaboration and cross-disciplinary foci Recommend research strategies and goals to the AIBN Board Identify unique funding opportunities for AIBN activities Assist in providing global visibility for AIBN activities Propose strategies for training and developing researchers and research students to build scientific capacity and capability in a multi- disciplinary, global environment.

AIBN Annual Report 2015 - 5 AIBN Research

6 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 7 Synthetic biology drives diagnostic care

Focus Highlights The Alexandrov Group uses protein engineering Of the many scientific publications produced by A three year NHMRC Development Grant to build entire biological expression systems the group in 2015, two were seminal papers. An was also awarded to further develop the using standardised biological blocks that can article in the Journal of the American Chemical Group’s work in point-of-care diagnostics for be integrated with electronics for application in Society used in vitro protein synthesis using monitoring the concentration and uptake of diagnostics and analytics. semi-synthetic tRNAs to formulate all 20 immunosuppressant drugs. common amino acids. A separate paper in Chemical Communications was the first to “Over the last eight years “Most of our work is centred demonstrate a functional electrochemical on signal detection and signal biosensor for human saliva using a we have had a 100 per cent repurposed glucometer, which used Ca2+ to success rate with securing ARC propagation. A key challenge electrochemically activate the sensor. is to detect and recognise Discovery Project grants we An international collaboration was established have applied for, so we know biomarkers of disease, which with the laboratory of Professor Andreas appear as very weak biological Plückthun at the University of Zurich, our work is being recognised for Switzerland. As international leaders in DARPin its need and output,” he said. signals in a very noisy, (designed ankyrin repeat proteins) research, the chemically similar background,” Plückthun Laboratory collaboration will assist Professor Alexandrov’s work was further Professor Alexandrov said. the Alexandrov Group to develop a pipeline acknowledged during the year with an for creating DARPins that can be used for cell Innovator and Vision in Research Award from signalling and receptor binding. Spin-out company Molecular Warehouse Ltd the National Breast Cancer Foundation (NBCF). has been established to further develop and This follows on from the award of an NBCF Major funding success in 2015 included an ARC commercialise this research, focussing on Innovator Grant in 2014 for research on point- Linkage Project with industry partner Phylogica point-of-care diagnostics that could, much of-care biosensors for detection of relapse in Limited for research studying the generation like glucose monitors for diabetics, enable breast cancer patients. and screening of highly diversified macrocyclic individuals to extract and analyse their own peptide libraries for use in pharmaceuticals. biological data. “We are still in the early days of our work Additionally, an ARC Discovery Project will with the NBCF, but I feel there is a compelling aim to develop a new class of electrochemical One stream of research is developing narrative there for the patient having more biosensors using artificial protein receptors. point-of-care sensors for monitoring control over their condition,” he said. immunosuppressant drugs given to organ transplant recipients. The therapeutic window for delivering these drugs requires extreme Alexandrov Group precision; over-dosage will shut down the www.aibn.uq.edu.au/kirill-alexandrov immune system and the patient dies from infection, and under-dosage can lead to the body rejecting the organ. The group has shown the sensors can detect clinically relevant levels of immunosuppressant drug Sirolimus, and is currently investigating detection of other commonly used drugs Tacrolimus and Cyclosporin.

In further work, the group are developing electrochemical sensors that could rapidly extract biomarkers from blood, saliva, urine or sweat to allow quantitative measurement of markers of various psychological and physiological conditions.

“One application area is detecting stress levels, which would be particularly important for monitoring people in professions subjected to high levels of stress that can drastically impact their work and the people they interact with as a part of their duties,” he said.

8 - AIBN Annual Report 2015 Using theoretical and computational science for nanomaterials and fluids Focus The Bernhardt Group develops theory and Bernhardt Group algorithms, and uses computational modelling www.aibn.uq.edu.au/debra-bernhardt to develop new materials and enhance the properties and capabilities of existing materials.

Utilising high performance computing, including a cluster housed at AIBN, the group models how materials, chemicals and biologics interact with each other.

Professor Debra Bernhardt said a prime example of the group’s work is its study of rechargeable batteries. Through computational modelling, the team is calculating how much lithium can be loaded onto an anode, how strongly it will bind, and how quickly it will diffuse.

Different cathode and electrolyte materials are also being studied as part of this process, and a paper published in The Journal of Physical Chemistry C highlighted the importance of the membrane structure and composition in determining its capacity.

“These relationships between materials help determine how long the battery will last in one cycle, as well as how many cycles of discharge and charge can occur through the battery’s life,” Professor Bernhardt said.

Further research was focussed towards Professor Bernhardt also leads the AIBN Work established with the Whittaker Group is optimising the effectiveness of materials used Centre for Theoretical and Computational investigating how temperature fluctuations in water desalination by studying the diffusion Molecular Science (CTCMS). The Centre has can influence the structure of polymers and of water through zeolites, which act as reverse high throughput computational facilities, and molecules, and determine how the molecular osmosis membranes. Work published in the leads collaborative activities with a number structure can influence the ability of a molecule Journal of Membrane Science found one of UQ schools and centres in the areas of to sit at interfaces between liquids such as dimensional cylindrical pores can result in algorithm development, materials, fluids, and oil and water. The collaboration with the Yu higher rates of diffusion. biomolecular systems. Group will research the mechanism by which surfactant molecules form micelles, and the “Atomic level modelling of the Highlights passage of molecules into the micelle for the diffusion of water and exclusion During the year Professor Bernhardt received synthesis of porous nanoparticles. a Window of Science Award from the United of ions for different zeolites States Air Force, supporting her to visit NASA In 2015 the group also oversaw can predict which zeolites and the Langley Air Force Base laboratories. the launch of a new high would be the most effective at The award was enabled through a continuing performance computational desalinating water,” she said. study funded by the Asian Office of Aerospace Research and Development (AOARD) – an arm facility, established with At a fundamental level, the Bernhardt of the Air Force Office of Scientific Research – $275,000 of funding received Group made a major breakthrough with the for research into the reinforcement of metals development of a new understanding of how such as aluminium and titanium with boron through the UQ Major systems relax to steady states, and these new nitride nanotubes to reduce the overall weight Equipment and Infrastructure theoretical relationships have been verified for and increase strength. the study of flow far from equilibrium. scheme. “The problem with any type of reinforcement, The dedicated machine has greatly enhanced Standard theories do not apply to however, is that if it slips or corrodes, it will throughput capability - allowing calculations nonequilibrium systems and interesting degrade the strength to a level that was lower that provide immediate access to high phenomena can be the direct result of the than it was originally, so we are investigating performance modelling and computations (full nonequilibrium nature of the system – for how to alleviate this,” Professor Bernhardt said. story page 33). instance, the alignment of polymers in solutions that are being sheared – leading the Bernhardt The year also saw the Bernhardt Group Group to develop theories and algorithms to commence new interdisciplinary projects with model nonequilibrium systems and investigate AIBN colleagues Professors Andrew Whittaker their behaviour at the molecular level. and Chengzhong (Michael) Yu.

AIBN Annual Report 2015 - 9 Biomaterials development and discovery for regenerative medicine

Focus Highlights The Cooper-White Group is focussed on Their engineered hydrogels are also being In 2015 the Cooper-White Group was improving health outcomes by utilising applied to promote repair in cardiac tissue. successful in achieving both philanthropic and biomaterials in regenerative medicine to assist Trials in mice are investigating whether competitive grant funding. the body’s ability to heal beyond its natural hydrogels developed by the Cooper-White limits. laboratory have the ability to stimulate cardiac repair by delivering small therapeutic “We were very excited to receive Led by Professor Justin Cooper-White, the molecules. Further potential applications for philanthropic support to test group studies novel methods to promote tissue these gels will be to study their suitability for regeneration in the bone, heart, intervertebral meniscal repair. our biomaterials system in large discs and the meniscus. animal trials. We really want Separately, microfluidic and micro-bioreactor “The body is quite remarkable in its ability to technology is being developed that screens to acknowledge this valuable heal itself. However, those capabilities only soluble environments to identify the optimal support, as it will enable us extend so far, and we are looking at ways setting required for cellular growth in to promote further regenerative healing,” tissues. Thousands of factors can be tested to generate essential data to Professor Cooper-White said. simultaneously using a nested fluidic circuit support our case of advancing that trials different chemical configurations of One strategy the laboratory is investigating is growth factors. this technology to pre-clinical the development of novel biomaterials that can trials in humans,” Professor direct stem cells to turn into the desired tissue “Our microfluidic technology could accelerate end points. the development of new media to discover new Cooper-White said. molecules that can enhance the differentiation An NHMRC Project Grant will support research During the year the group focussed on in vivo process of cells,” he said. to deliver targeted nanoparticles containing validation and assessment of its methods genetic material to repair cardiac cells that using animal models. The research team looks to apply that knowledge to the earliest stages of cellular have been damaged through ischemic heart disease, which accounts for 15 per cent of “We have large animal trials in sheep currently development from pluripotent stem cell Australian deaths every year. The project aims in progress, assessing the efficacy of our starting points, and guide their specification to transform a patient’s damaged cardiac hydrogels to elicit chondrogenesis locally to into kidney, cardiac and other tissues such as fibroblast cells into functional cardiomyocytes repair cartilage within the intervertebral disc,” cartilage and skeletal muscle. in situ. he said. Similarly, the group is continuing research into Publication highlights from the year included a Future large animal model trials will utilise kidney genesis using directed differentiation paper in Biomacromolecules, which highlighted porous scaffolds which have been developed of human embryonic stem cells as kidney a significant improvement in the lifespan and modified by the laboratory to promote progenitors. of self-assembled pseudopolyrotaxane meniscal repair. hydrogels from a few hours up to one week. The team achieved this result by introducing an enzymatically mediated chemical bond with a branched eight-arm poly(ethylene glycol) linked to the hydrogel structure to improve its environmental resistance. A review article was also published in Stem Cells Translational Medicine on the topic of tailoring bioengineered scaffolds for stem cell applications.

Professor Cooper-White received recognition from Queensland Life Sciences – accepting the 2015 Aon Risk Solutions Regenerative Medicine Award. Separately, he was elected to the Queensland Academy of Arts and Sciences, where he joined fellow AIBN Group Leader Professor Matt Trau and retired Group Leader Professor John Drennan.

Professor Cooper-White holds a joint appointment with the CSIRO as an Office of the Chief Executive Science Leader, and is Director of the Australian National Fabrication Facility - Queensland Node.

Cooper-White Group www.aibn.uq.edu.au/justin-cooper-white

10 - AIBN Annual Report 2015 Manammali cell expression and bulk stem cell cultivation

Focus The Gray Group is developing novel methods “By raising the temperature again, the smaller enable the laboratory to expand its to reduce the cost of producing monoclonal aggregates of stem cells once again grow into efforts in understanding how it will be antibodies for therapeutics, and significantly larger aggregates. By repeating the process you possible to take the work with the thermo- boost the production yields of stem cells for get an exponential increase in cell yield using responsive polymers and develop scalable use in regenerative medicines. very benign conditions which the cells tolerate processes which will allow sufficient stem cells well.” to be produced for future clinical applications. Professor Peter Gray said many modern pharmaceutical products rely on complex The team hopes to initially use stem cells recombinant proteins, most of which are for treating injured heart muscle with a The group’s expertise led to manufactured in mammalian cell culture. differentiated progeny as cardiomyocytes. a new collaboration being Another application area being investigated is “The cost of supplying these is very high, their use as neural progenitor cells, which have established with Translational however, given their efficacy such products been difficult to produce, but there are hopes Cell Imaging Queensland at the now make up seven out of the top 10 selling that the thermo-responsive polymer method pharmaceuticals globally,” Professor Gray said. developed by the Gray and Monteiro Groups Translational Research Institute, could resolve this challenge. which will investigate methods “Australia alone is spending for high density 3D imaging of Highlights billions of dollars on During the year the team was involved with cell aggregates and examine therapeutics such as these the Phase I clinical safety trials of the m102.4 their organisation. Hendra virus antibody which it assisted in under the pharmaceutical producing for Queensland Health, with the trial In December Professor Gray took the esteemed benefits scheme, and a lot our progressing well. position of Interim President of the Australian Academy of Technological Sciences and work is in developing ways to Outbreaks of Middle East respiratory syndrome Engineering (ATSE), one of four learned lower the manufacturing cost of (MERS) also led to collaborative talks between Academies in Australia. Professor Gray, Queensland Health, AIBN, and US collaborators. who served on the ATSE board for six years, these drugs.” The US researchers from the National Institutes replaced Dr Alan Finkel who was appointed as of Health, who were involved in the initial Australia’s Chief Scientist. In collaboration with the Nielsen and Mahler development of the anti-Hendra virus antibody, Groups at AIBN, continued research in have now developed a monoclonal antibody improving production yields of therapeutic against MERS (full story on page 30). monoclonal antibodies (mAb) using Chinese hamster ovary (CHO) cells as a host The year also saw philanthropic research was published in Metabolic Engineering funding from the Merchant Charitable Communications and the Journal of Proteome Foundation and the JEM Research Foundation Research in 2015.

This research program using CHO cells produced a four-fold improvement in mAb Gray Group yields and six-fold improvement in culture volumes. These results demonstrate www.aibn.uq.edu.au/peter-gray economically significant advances to reducing the production costs of high-expression cell lines.

In further work, the Gray Group combined their expertise in stem cells with the Monteiro Group’s knowledge of materials to address the significant challenge of achieving desirable properties when growing these extremely fastidious cells.

The Monteiro Group has developed a thermo- responsive polymer that allows an aggregate of cells to stick together and promotes cell growth and replication at 37oC. When followed by a reduction in temperature, the aggregates divide when subjected to mild shear forces.

AIBN Annual Report 2015 - 11 Biopolymers and starch for a cheaper, cleaner future Focus Highlights The Halley Group are leaders in the field derived from wastewater treatment for value- During the year Professor Halley’s spin-out of biopolymer processing, with strengths added use in the production of biodegradable company Plantic Technologies, which produces in developing materials with lower carbon plastics. biodegradable plastic packaging from corn footprints. starch, was purchased by Japanese company Another new area of research is investigating Kuraray Co. Ltd following 20 years of research This year saw the group led by Professor Peter whether the hydrophilic nature of abundant and more than $75 million in venture financing. Halley embark on a number of new research starch is suitable for use in extractive programs. metallurgy to separate minerals from their ore. “The company was spun-out in 2001, Led by Dr Paul Luckman, the work is being and started funding the group with PhDs undertaken with industry partner Manildra and projects to continue the research and “Traditionally our work has Group in Nowra, NSW. development, alongside the company’s strong been in starch polymers for use domestic and international growth,” Professor Current extraction techniques use synthetic Halley said. in films and packaging, but a chemicals to separate minerals from their ore. number of new opportunities While existing methods are effective, they are “The acquisition of Plantic Technologies is truly costly due to the expense of the chemicals – a an example of commercial success being led have arisen from what we had cost that is more pronounced while commodity by university research.” learned from that research,” prices are low. Dr David Fengwei Xie from the Halley Group Professor Halley said. “Our work has been very promising, but we has also played a leading role in strengthening are still looking to understand exactly why this the laboratory’s international linkages, with In the first of those new areas is research process works, and whether we could apply it collaborations formed with the South China into bio-based composite materials led by Dr to other areas,” Professor Halley said. University of Technology (SCUT) and the Bronwyn Laycock. University of Strasbourg, France. “This has created a whole different area of The group is further The productive collaboration has involved composite materials that combines a polymer joint students, laboratory exchanges and joint with a fibre to create a stronger material,” investigating the use of publications, including a paper published with Professor Halley said. starches for improving the SCUT in Innovative Food Science and Emerging Technologies on the pasting properties of Dr Laycock led new projects in 2015 at the adhesive properties of glues on mechanically modified high-amylose corn UQ School of Chemical Engineering, the UQ cardboard to develop stronger starches. Dr Xie’s work is centred on new Dow Centre for Sustainable Engineering biopolymer systems such as chitin and the use Innovation and the QLD Advanced Materials and more robust packaging. of ionic liquids as novel biopolymer plasticisers. Processing and Manufacturing (AMPAM) Centre. One stream of research, with findings Following his appointment as Head of the UQ published in Water Practice and Technology, is School of Chemical Engineering, Professor evaluating the economic feasibility of using Halley has transitioned his work to the School- polyhydroxyalkanoate (PHA) biopolymers based AMPAM Centre.

Halley Group www.aibn.uq.edu.au/peter-halley

12 - AIBN Annual Report 2015 Gene and drug delivery and diagnostics through the skin

Kendall Group www.aibn.uq.edu.au/mark-kendall

Focus The Delivery of Drugs and Genes Group is “In animal studies, we have shown the Moving from vaccination to disease diagnosis, prompting the world to rethink the role of Nanopatch generates equivalent and protective the group advanced a micro projection the needle and syringe as a method of both immune responses as the needle and syringe technology which selectively extracts introducing vaccines to the body and extracting (into muscle), but with only small fraction biomarkers from the skin – with minimal biological material. of the delivered dose (e.g. 1/100th). Steps invasion – for the detection of disease. Work are underway to translate this to humans,” published in Biointerphases demonstrated in Group Leader Professor Mark Kendall is Professor Kendall said. vivo application of the system with successful inventor of the patented Nanopatch technology, detection of the dengue NS1 disease biomarker which has many important benefits over the Professor Kendall founded Vaxxas in 2011, (full story page 32), followed by new insights more than the needle and syringe, which with the aim of taking the Nanopatch through into how to gain access to blood through the – more than 160 years after it was first clinical testing and along the commercial use of physical stress in the skin (published in invented – is still the main way vaccines are pathway to a product in market. Biomaterials). administered. Vaxxas are scheduled to conduct vaccination clinical trials with Nanopatches in 2016. The The group’s line of research “The Nanopatch has the technology has also been licenced to Merck & inquiry extends at the potential to significantly Co for the development of further vaccines to work in conjunction with the Nanopatch. interface of biomaterials and improve the reach of effective immunology, including 3d vaccines, by overcoming key Highlights printing of microstructures, limitations of the needle and Working with the World Health Organisation, US Centres for Disease Control and Prevention their utility in epithelial tissues syringe,” Professor Kendall said. and Vaxxas, Professor Kendall’s team has and the interrogation of key been testing the utility of polio vaccines with Because the Nanopatch is designed to target Nanopatches. They have shown that in rats, interactions (e.g. with systems the immune-rich cells of the skin’s outer layers using just a fraction of the dose that is required biology and proteomics). (the epidermis and upper dermis), it offers with a needle and syringe, the Nanopatch improved immune responses, compared to the generates functional immune responses In 2015 Vaxxas secured a further $25 million needle which puts vaccine into muscle (which (publication in Scientific Reports). in Series B venture financing to extend the has comparatively fewer immune cells). It development of the Nanopatch for further does this with an array of thousands of micro Furthermore, the group published work clinical platforms and vaccines for diseases. projections – invisible to the naked eye – on a in Vaccine highlighting the first use of a single patch. pneumococcal-conjugate vaccine delivered using micro projections, achieving through Vaccines are dry-coated onto the patch, targeted delivery to the skin improved helping to eliminate the need for the vaccine functional immune responses compared to the cold chain which is both expensive to maintain, needle and syringe. Pneumonia is a leading and risks being broken in underdeveloped cause of death in children globally under the regions that have poor access to electricity age of five, and was estimated to cause more networks. than 900,000 childhood deaths in 2015.

AIBN Annual Report 2015 - 13 Biologics for medical applications

Focus The Mahler Group is working in drug discovery “In particular we are focussing on therapeutic The work is being primarily and development, principally researching and diagnostic applications of antibodies and biologic medicines and nanomedicines for the antibody derivatives, which are engineered applied to the development of treatment of human disease. proteins built from monoclonal antibodies,” antibody-targeted nanomedicines Associate Professor Mahler said. The research led by Associate Professor and theranostics, with a number Stephen Mahler has a focus on the discovery of “The approach of using nanoparticles laden of influential collaborations new therapeutic proteins, as well as developing with drugs targeted at tumours, with our drug delivery platforms to precisely target antibodies on the surface, provides an advancing the Mahler Group’s cancer cells. added targeting capability to drug-loaded research. nanomedicines.”

Mahler Group www.aibn.uq.edu.au/stephen-mahler

Highlights A provisional patent “Targeting Constructs Mahler also led a paper published in mAbs, “Cpn10 is a new potential drug for the for the Delivery of Payloads” with co-inventor which investigated nanocell targeting using treatment of inflammatory and autoimmune Dr Chris Howard was filed in April 2015 and engineered BsAbs, which enabled enhanced disorders, so this is providing further avenues has advanced to the PCT stage to provide tumour regression by 40 per cent. for our research to progress in new areas,” he international legal protection. The invention said. is associated with the discovery of systems In other work associated with antibody for the targeted delivery of nanoparticles to research and development, an international A Research Connections Grant was also cells using bispecific antibodies (BsAbs). The (PCT) patent application “CD83 antibodies secured during the year through AusIndustry technology will be used to target promising and use thereof” entered the national phase in to continue work into targeted delivery of prostate cancer antigen MIL38 in a new Australia, New Zealand, Europe, Japan, South nanoparticles for therapy with Sydney-based partnership with Minomic International Ltd. The Korea, Mexico and the United States. The company EnGeneIC. This follows a previously work also offers hopes as a pancreatic cancer parent antibody 3C12 was first isolated at the successful ARC Linkage Project. therapy. National Biologics Facility at AIBN, with AIBN co-inventors Dr Martina Jones and Dr Trent The success of the group’s work in antibody Munro (now at Amgen). discovery and drug delivery also led to a new “We had an existing relationship collaboration with Australian pharmaceutical with Minomic International Ltd Further success was achieved with the first leader CSL Limited, which will begin in early royalties arriving for the licensing of 3C12 as a 2016. through prior work, and we therapy against graft-versus-host disease. The were very happy to secure an group’s work on CD83 saw research published “It is very gratifying to see momentum building in the high impact factor journal Leukemia, led behind this research, and we are hopeful of ARC Linkage Project grant with by Professor Derek Hart of the University of rapidly advancing these technologies,” he said. them led by Associate Professor Sydney, demonstrating that 3C12 can be used as an immunosuppressive therapy in organ In December, Associate Professor Mahler and Kristopher Thurecht to continue transplantation. AIBN colleague Dr Simon Corrie organised a our research,” Associate successful workshop hosted at UQ entitled In the area of bioengineering and biologics “Biomedical Applications of Engineered Professor Mahler said. production, a paper in Process Biochemistry Antibodies and Proteins”, attracting many studied the production and characterisation research leaders and industry representatives Related work utilising BsAbs saw the group’s of an anti-inflammatory biologic, recombinant from around Australia, leading to plans to hold involvement in a successful NHMRC Project human chaperonin 10 (Cpn 10). Ala-Cpn10 the event annually. Grant with Associate Professor Kristopher is undergoing phase two efficacy trials as a Thurecht on immuno-polymeric drugs for potential treatment of autoimmune disease prostate cancer therapy. Associate Professor systemic lupus erythematosis.

14 - AIBN Annual Report 2015 Advances Make Plastic Truly Fantastic

Focus A significant amount of plastics, rubbers and carbon fibres are unsustainably sourced from petrochemical-derived materials, which consume limited resources and adversely impact the environment.

The Martin Group, led by Professor Darren Martin, is investigating ways to not only replace these materials with sustainable alternatives, but in many cases enhance the performance compared to existing products.

“We established a company called Tenasitech Pty Ltd in 2009, which has developed polymer nanocomposites targeting products such as scuff resistant golf balls and scratch and mar resistant headlight lenses and kitchen cupboard doors, but without the need for solvent-based ‘hard coats’,” Professor Martin said.

“However, recent developments have seen us significantly extend the sustainable, scalable, and high performance nanomaterial theme of our research into new and truly exciting areas.” Martin Group A discovery in the Martin laboratory found www.aibn.uq.edu.au/darren-martin an economically viable and environmentally friendly method of extracting nanocellulose fibres derived from the Triodiapungens carbon fibre research facility. The team is “In Northern Queensland, for instance, bitumen species of Australian spinifex grasses. This studying the suitability of nanocellulose as nanocellulose can be used as an additive to roads quickly develop ruts with extreme an additive to create more renewable, cost- temperatures and use by heavy vehicles. If a enhance the strength and toughness of a range effective nanocomposite precursor materials – of materials, offering numerous commercial road lasted twice as long, you would save a lot and convert these to high performance carbon of money.” opportunities. fibre (full story page 31). A study in RSC Advances compared spinifex The technology is also being investigated “There is growing use of carbon fibre due to improve water filtration, which ideally nanocellulose to other sources of cellulose, and to its strength and light weight, but cost is found its unusually high remnant hemicellulose provides for high flux – allowing a lot of water the restriction between it becoming more through, but also separating fine particles content is responsible for the significant mainstream, and the spinifex nanocellulose “effortless fibrillation” characteristics it or nasty bugs. Together with Stony Brook looks like it could play a significant role in University, USA, the group is studying whether displays. A further study in Cellulose highlighted reducing those costs,” he said. the ease of extraction, requiring minimal energy the nanocellulose could be used to develop a input to deconstruct the spinifex leaves into cheaper and possibly even higher performing Another industrial collaboration sees the membrane. cellulose nanofibrils with an average diameter group working with Sydney-based Derby below 10nm. Rubber Products Pty Ltd to investigate manufacturing stronger compounded rubber During the year group also Highlights using nanocellulose in applications such tyres gained support from Cook In 2015 an umbrella agreement with Dugalunji and traffic counter tubes. Compounded rubber Aboriginal Corporation (DAC) was signed to products wear down via abrasion, and also tear Medical Australia Pty Ltd for ensure ongoing equity between UQ and DAC and deform through the application of various research and development of in the development and commercialisation of forces – particularly under elevated service the nanocellulose technology. Development temperatures. The improvement of these polymeric materials for medical of a commercial spinifex feedstock industry products could lead to commercial avenues applications. could provide new and sustainable economic in the $200 billion global compounded rubber opportunities for remote communities. market. The efforts of Tenasitech Pty Ltd were recognised as a finalist in the 2015 Telstra The research team was awarded a three year The work could also be extended to civil Australian Business Awards. ARC Linkage Project with CarbonNexus at infrastructure applications as an additive for Deakin University to investigate the additive’s bitumen road surfaces to improve durability, utility in remodelling the way carbon fibres after research found nanocellulose increases are made at the world’s first university-based the dimensional stability of bitumen.

AIBN Annual Report 2015 - 15 Self-assembling soft condensed materials science

Middelberg Group www.aibn.uq.edu.au/anton-middelberg

Focus Highlights The primary research theme of the Middelberg In a world first approach, work led by During the year the Middelberg Group was Group is the engineering of self-assembling ARC Future Fellow Dr Chunxia Zhao of awarded an ARC Discovery Project grant bio-molecular systems. the Middelberg Group has developed a to study vaccine delivery for pathogenic recombinant catalytic modular protein that can viruses. In a high value collaboration for Group Leader Professor Anton Middelberg said take a base oil droplet at room temperature and AIBN and UQ, the grant will establish a major that the laboratory’s work uses bio-inspiration encase it with silica formed out of 116-residue strategic partnership with UQ Gatton and to drive new functional outcomes in materials protein D4S2. CSIRO’s animal sciences facility in Geelong, sciences and health. which is one of only a handful of high level This design creates a barrier that protects biocontainment facilities worldwide. “When people think of nanomaterials, they the cargo, and provides control over the tend to be perceived as inorganic, hard rate at which the cargo is released into the “There are some fundamental science questions materials; whereas our group primarily surrounding environment. If used in agricultural underpinning the system that can really radically intersects nanomaterials with a field called soft applications such as crop protection, for change how we do vaccine delivery, and the condensed matter,” Professor Middelberg said. example, the system allows for a prolonged vaccine field has been very conservative without treatment period with a lower environmental a lot of innovation from the manufacturing side,” “It is the meeting of polymer chemistry and impact than traditional pesticides (full story Professor Middelberg said. protein chemistry for the design of self- page 34). assembling systems such as virus like particles In 2015 the group published a study in Vaccine, (VLPs), with multiple layers of self-assembly to Dr Frank Salisbury is furthering this targeted with Associate Professor Linda Lua as first create entirely new functional structures.” nano-emulsion delivery technology with self- author, which used the laboratory’s self-created assembling oil droplet technology for health- vaccine platform to develop a VLP against The group has developed a platform related applications. rotavirus. The illness is responsible for around technology that modularises VLPs with entirely 400,000 deaths annually. new antigenic modules to drive favourable Nanoparticles are traditionally solid and unable antibody and cellular responses in both human to pass through narrow in vivo obstacles such The study showed strong immunogenicity and animal diseases. as blood capillaries, whereas the deformable results for the VLP when paired with an antigen oil droplets can carry a therapeutic cargo that as large as 18kDa in size. The VLP removes the could distort to pass through. Use of such need for a vaccine adjuvant, has the potential The development of soft carriers would allow for quicker and wider to be cheaper to produce than existing templated nanocapsules has circulation through the body, and consequently vaccines, and is not dependent on the vaccine require lower doses of therapeutics. also led to new discoveries in cold chain. smart reaction systems. “The human body is a collection of soft The research team chose to publish this work matter, so this focus on exploring deformable, in an open access journal due to its potential condensed soft matter systems is for to significantly impact human health globally, the creation of more robust drug delivery and to facilitate the work to progress towards platforms,” Professor Middelberg said. translational pathways.

16 - AIBN Annual Report 2015 Synthesis and application of complex polymer architectures Focus Highlights The Monteiro Group takes a holistic approach spherical shape. Recent advances have In 2015 the group built on previous work towards polymer chemistry, understanding the enabled the creation of a range of shapes from published during 2014, which developed fundamentals of kinetics and thermodynamics loops, rods, worms, vesicles, and now a bi- nanoparticles in the shapes of worms and of polymers to create complex polymer compartmentalised tadpole nanostructure. rods. They were the first to demonstrate the architectures that can be custom designed to creation of polymeric tadpole structures, with a suit specific applications. “We can now put chemical functionality onto paper appearing in the Journal of the American these custom designed nanoparticle structures Chemical Society. Group Leader Professor Michael Monteiro said by binding molecules such as fibronectin and the approach has allowed the group to design vitronectin on the surface,” he said. complex dendritic structures using polymeric “With the tadpole structure, building blocks. The group is collaborating with the Gray the tail can have a different Group at AIBN to use these types of polymeric “We use living radical polymerisation nanostructures to study cell expansion for functionality to the head, which techniques, including SET-LRP, which allows stem cell research. can be important in vaccine and us to create polymers with very high chain-end functionality to connect polymers together,” Work previously published in drug delivery where the optimal Professor Monteiro said. Biomacromolecules (2014) demonstrated application requires more than the novel growth of human embryonic stem Papers published during 2015 in Current Drug cells (hESCs) in suspension cultures using the one agent to be administered,” Delivery and the Journal of Medicinal Chemistry Monteiro Group’s thermo-responsive polymer Professor Monteiro said. with Professor Istvan Toth and Dr Mariusz worm nanostructures. Skwarczynski of the UQ School of Chemistry Further work published in the European Polymer and Molecular Biosciences successfully “Together with the Gray Group we have clearly Journal developed methods to accurately demonstrated the use the Group’s polymers demonstrated that hESCs can be built, cooled measure the molecular weight of polymers as carriers for vaccine and drug delivery. The down and then broken up using our polymer using size exclusion chromatography (SEC). respective findings showed decreases in nanoworms as a suspension culture for The paper received more than 3,000 views in tumour size and eradication of E7-positive TC-1 scaled-up production,” Professor Monteiro the first six months following its publication, tumours in a murine human papilloma virus said. indicating wide scientific appeal. tumour model. The research team reported a more than 30- Findings published during the year in Polymer Taking their work of complex structures further, fold expansion in cell numbers over 18 days Chemistry (RSC) in collaboration with Professor the group studies emulsion polymerisation to with the enzyme-free propagation approach, Virgil Percec (University of Pennsylvania) make nanoparticles beyond the traditional while critically maintaining cell pluripotency. provided the strongest evidence for Copper(II) oxide (Cu(0)) activation in water, resulting in the preparation of water-soluble polymers directly Monteiro Group in water. Both propagation and reversible www.aibn.uq.edu.au/michael-monteiro deactivation steps occur while the polymer radical is adsorbed on the surface of the Cu(0) catalyst.

During the year work continued on existing grants, including an ARC Discovery Project on precision-engineered polymer nanomaterials, which is linking synthetic materials and biological systems. Work was also undertaken on developing highly productive and selective bio-organic hybrid membrane water filters, through a grant awarded by the National Centre of Excellence in Desalination Australia.

Professor Monteiro is an editor of the European Polymer Journal, and also accepted a position as an editor of the Heliyon journal in 2015. He is also on the advisory boards of Biomacromolecules (ACS) and the Journal of Polymer Science Part A: Polymer Chemistry.

He was an organiser of two symposia at the Pacific Chem Conference held in Hawaii, USA, in December and over the year also delivered three plenary lectures at international conferences in the USA, Mexico and Greece.

AIBN Annual Report 2015 - 17 Systems biology to unlock biological complexity Focus The Human Genome Project inspired the tools for better understanding (analysis); “Studying such a diverse range development of high throughput, low cost they use these tools to design products and ‘omics technologies. We can now sequence processes for human benefit (synthesis). of organisms is challenging and a microbe in a morning and prepare a The science is moving from crude retrofitting is only practical through close comprehensive molecular inventory (expressed of living systems with a single or few genes genes, proteins, metabolites) in a week. using genetic engineering to purposeful re- collaboration with field experts,” However enumerating the components in engineering of living systems using systems Professor Nielsen said. a biological system does not in itself yield and synthetic biology. understanding. “However, the advantage is that knowledge Drawing on a common core of expertise in gleaned from one system can lead to The Nielsen Group uses systems biology, that genome scale metabolic modelling and the full unanticipated findings in unrelated organisms.” is systems science, modelling and simulation, range of ‘omics technologies, the group studies to integrate data streams and generate a diverse range of biological systems, including knowledge. As applied systems biologists or model and industrial microbes, animals cells, biotechnologists, they do more than develop plants and increasingly complex ecosystems.

Nielsen Group www.aibn.uq.edu.au/lars-nielsen

Highlights The group produced more than 20 peer- to build a detailed kinetic model explaining Australia on improving production techniques reviewed publications during the year. Of why cancer cells and other fast-growing cells for animal toxoid vaccines. The project particular significance was a paper published in produce lactic acid. continuous a successful pilot project and aims high impact factor journal PLoS Computational to reduce the number of batch failures and Biology, which employed a General Reaction increase the concentration and quality of the Assembly and Sampling Platform (GRASP) “The grant enables me to vaccines. developed within the laboratory to consistently return to the problem that first parameterise and sample accurate kinetic Work also commenced on a new ARC Linkage models of enzymatic reactions. The framework attracted me to a career as Project with Lanzatech on gas fermentation, in allows for complex kinetic modelling without a research scientist, namely particular the use of systems biology to guide requiring large data points to parameterise strain engineering. The project has already enzyme kinetics under non-equilibrium how do we develop a tractable produced the first comprehensive systems conditions. model of the complex set of biology platform for gas fermenting bacteria and the first patent application. 2015 was a successful year for securing biochemical reactions within a funding for the group. Professor Nielsen was human cell,” Professor Nielsen Work continued on an existing ARC Linkage awarded a $AUD8.6 million Novo Nordisk Project with The Dow Chemical Company on Foundation Laureate Research Grant, which said. propionic acid production leading to very high will see him develop computational models of yielding strains and a patent application. During the year the group was also awarded an mammalian cell metabolism. The aim is ARC Linkage Project grant to work with Zoetis

18 - AIBN Annual Report 2015 Point-of-care diagnostics to save lives

Focus Highlights In further work published on the cover of The Centre for Personalised Medicine is 2015 was a successful year for the Trau Group, Chemical Communications, a collaboration dedicated to developing improved point-of-care seeing broad research outcomes and grant with Professor Jimmy Botella from UQ’s School diagnostics from the benchtop to bedside, success provide a foundation for further work of Agriculture and Food Sciences developed a with the goal of significantly enhancing patient to continue. single-drop DNA test that can detect disease in outcomes and helping to transition the medical crops and livestock, as well as human diseases. system towards early disease detection and The group secured a new ARC Discovery personalised treatment. Project grant to develop a platform technology to characterise nano-scaled objects on a “The test uses fully automated particle-by-particle basis in situ. Driven by a molecular/nanoparticle By understanding disease need across broad industries to understand the epigenetics and combining distribution, charge and composition of nano- systems within a single drop of particles, the project aims to design a system fluid to analyse a sample, detect chemistry with nanotechnology, to accurately ascertain these features. Professor Matt Trau said the the presence of a pathogen, and More than 20 peer-reviewed journal articles in 55 minutes provide a positive Group’s research aims to were published by the lab in 2015, including provide improved accuracy over two papers in the Nature family of journals and or negative result,” Professor a front cover article in the journal Chemistry Trau said. existing diagnostic devices, at a Communications. In collaboration with the Garvan Institute of Medical Research, cheaper price. The single-drop test has been proven to work work published in Nature Communications in human diseases such as prostate cancer, “We have a strong history of developing described the study of the responsive enhancer HIV and malaria, as well as bovine herpes virus diagnostics that use novel biomarkers to detect methylation of oestrogen receptor 1 (ESR1). in cattle, and fusarium fungus in crops. Another the presence of diseases such as cancer,” The presence of ESR1 determines whether avenue of development includes a cost- Professor Trau said. a breast cancer patient receives endocrine effective tuberculosis screening test for use therapy, but does not predict the success of in developing countries, which resulted in the The group engineers platforms that allow treatment. The findings showed that ESR1- publication of three papers during the year in biological data to be collected and analysed responsive enhancer methylation status could ACS Sensors, Scientific Reports, and Analytical outside of clinics – with the goal of providing potentially be used to classify patients as Chemistry (full story page 35). patients with an enhanced quality of life, while responders to endocrine therapy. monitoring disease progression, regression, or reoccurrence. Trau Group The group has been strongly supported by the National Breast Cancer Foundation, with www.aibn.uq.edu.au/matt-trau successive grants of $5 million funding the development of next generation breast cancer diagnostics. An Australian Research Council Discovery Project grant is also providing the opportunity to develop accurate methods of capturing specific cells and molecules, such as low concentration pathogenic molecules and circulating tumour cells (CTCs).

CTCs shed from cancerous tissue and travel away from a primary tumour site through the bloodstream, eventually establishing secondary sites that speed the progression of cancer in the body.

“As hazardous as CTCs are, they provide us with a biomarker that we can detect in the blood stream, which can help tailor more effective and personalised treatment of cancer,” Professor Trau said.

“We are very passionate about inventing highly innovative technologies that can be translated into the clinic. We thank all of our supporters, funding agencies, charitable organisations, industry partners and philanthropic donors.”

AIBN Annual Report 2015 - 19 Development of solar conversion and storage materials for a greener future Focus Highlights The Wang Group is focussed on the clean 2015 was a successful year for the group, Additionally, Dr Yu Chen from energy sector, developing new functional with a number of publications in high impact materials for solar energy conversion and journals and successful funding outcomes. the Wang Group secured storage systems. a highly competitive ARC Dr Delai Ye published a new design for a Discovery Early Career Led by Professor Lianzhou high capacity lithium-ion battery using a new cathode material in Advanced Functional Research Award for the Wang, who also directs the Materials. The design allows for around a 30 development of a multi- Nanomaterials Centre that per cent capacity increase, with more than 800 cycles without significant degradation in functional nanoparticle system, was established at UQ in 2000 performance. which is expected to lead to by former AIBN Group Leader In work published in Advanced Materials exciting new progress on a Professor Max Lu, the group is with Dr Eun Ji Yoo, Mr Miaoqiang Lyu and diagnostic and therapeutic building on the Centre’s strong colleagues reported the first example of using organic-inorganic hybrid perovskite materials system to combat cancer. legacy. in resistive random access memory devices. An ARC Linkage Project grant was also “One of my main research areas has been in Another paper in Advanced Energy Materials awarded mid-year for a partnership with solar hydrogen generation, and around half by Dr Qiong Wang highlighted a new design UQ’s School of Mechanical and Mining of the team is working on photocatalytic for a solar battery, combining the concept of Engineering and Baosteel Company to develop hydrogen production,” he said. a solar cell and rechargeable battery in one nano-lubricants that can be used for steel integrated system by using lead and iodine on processing. Dubbed artificial photosynthesis, the splitting one electrolyte. of water to produce hydrogen is a very “We are investigating the development of promising solution to address renewable “We are very excited by the potential of this nanoparticle suspension to replace oil-based energy utilisation and climate change work, but further progress is needed in the lubricants that would be more environmentally abatement. capacity and energy conversion rate. If this friendly,” he said. can be addressed it would be a very elegant The group is also actively developing new system,” Professor Wang said. Professor Wang was also honoured during the advanced rechargeable battery technologies to year with his inclusion in the ARC College of improve existing lithium-ion batteries as well as An ARC Discovery Project was awarded for Experts for 2016-2018 within the Engineering, discover competitive alternatives. work on a new bifunctional photocatalytic Information and Computing Sciences discipline reaction system, which will provide new grouping. opportunities to convert industrial waste water “Lithium-ion batteries are the into solar hydrogen and valuable chemicals state-of-the-art technology such as bromine using solar energy. in rechargeable batteries and

are quite effective, but lithium Wang Group is a very limited resource so www.aibn.uq.edu.au/lianzhou-wang the world cannot rely on it for our energy storage needs,” Professor Wang said.

To address this, the team is developing new materials for a next generation sodium- ion battery. Although originating from a far more plentiful source, there are substantial technological hurdles to overcome before it can genuinely challenge lithium-ion in the marketplace.

“The sodium-ion is bigger, so the charge has been slower and the capacity is smaller. However, during the year our group made significant inroads by developing a prototype sodium-ion battery with comparable capacity and charge times to lithium-ion ones.”

20 - AIBN Annual Report 2015 Big data shows big stem cell possibilities

Wells Group www.aibn.uq.edu.au/christine-wells

Focus Highlights The Wells Group is seeking to unlock the long As part of a large international research By taking hundreds of data sets from around term potential stem cells have promised for the consortium named FANTOM (Functional the world the group’s work with Stemformatics last four decades. The team primarily uses a Annotation of the Mammalian Genome), in found a genetic signature – 16 genes that are computational approach to analyse enormous 2015 the group published a paper in Science. like a fingerprint that highlight whether a cell is datasets. actually a stem cell. Using a large-scale comparative analysis, the Professor Christine Wells said the group is paper describes how stem cells are activated The group also partnered with Bioplatforms addressing key questions that still need to be by a DNA “switch” and genetic information. Australia to work with eight different answered about stem cells, which, functionally, The cascade of changes that occur to a laboratories around Australia to establish a can become any other type of cell in the body. mammalian cell during transcription are large new data set. $1 million in funding across triggered by enhancer transcription, followed the labs for 18 months will enable research “In every cell of your body there are stem cells by the production of enhancer RNA. into stem cell biology, with the Wells Group that are critically important for how you age taking on the role of coordinating the data and how your body repairs itself, and there are “I think this will continue to be a very major gathering, and processing the data through the also implications for disease. So understanding resource for the entire research community for Stemformatics platform. this process is critically important,” Professor some time because the depth of information Wells said there is very rich,” Professor Wells said. Professor Wells’ achievements were recognised during the year with the award “It remains to be properly understood as to of the prestigious Metcalf prize for stem what mechanisms dictate whether a stem cell The FANTOM consortium has cell research, and a prestigious ARC Future converts into kidney or a beating heart.” previously published work in Fellowship to undertake a systems biology approach to understand the complex cell-to- In a research field known as computational Nature and Science. cell interactions of cellular populations. This genomics, the team grapple with incredibly work to identify new classifiers will further In 2015 the group’s international online vast amounts of data, dealing with millions of assist in identifying the differentiation potential platform Stemformatics.org grew to around data points at any given time. of stem cells. 7,000 users a month. The platform – a collaboration with Stem Cells Australia – The group is a member of Project Grandiose, NHMRC funding also supported work to processes and presents data to stem cell led by Dr Andras Nagy of the Lunenfeld- investigate stroke and stroke outcomes, a researchers and clinicians in a way that is Tanenbaum Research Institute, Canada, research theme led by Dr Silvia Manzanero. easily understood. It hosts hundreds of data which identified new F-class stem cells in Additionally, in work supported by the ARC, Dr sets, and thousands of different stem cells a 2014 Nature paper. These artificial cells Florian Rohart is looking to build new statistical have been identified within the platform. only exist in culture and are formed from the tools to continue improving data mining reprogramming of somatic cells in elevated techniques. Successful application of the Stemformatics reprogramming factor expression. Studies platform answered one of the biggest are continuing into F-class stem cells to gain Professor Wells has held a joint appointment as challenges the field has faced: identifying what insights into the formation of stem cells. a reader at the University of Glasgow’s Institute is a stem cell and not an already differentiated of Infection, Immunity and Inflammation. cell assigned to a particular role.

AIBN Annual Report 2015 - 21 Innovative polymer chemistry for health and IT Focus The Whittaker Group applies synthetic methods the work is being done in collaboration with on fundamentals of thin polymeric films will to develop technologies for health and our neurosurgeons who implement the findings. help to further build this program. modern technological society. These include advanced next generation molecular imaging Another important area of nanomedicine being Amongst the numerous papers published agents for disease detection, and polymers addressed by the group is the development from the group during the year, PhD student to be used in advanced lithography for the of anti-microbial surfaces to remove or kill Cheng Zhang’s work on thermoresponsive fabrication of integrated circuits. microbes on surfaces to reduce hospital-born poly(OEGMA-co-TFEA) in the leading research infections. journal Macromolecules was a standout. The Professor Andrew Whittaker said future copolymers, synthesised using reversible imaging agents injected into the body and “Around 30 per cent of patients admitted addition-fragmentation chain transfer (RAFT) imaged using MRI, CT or fluorescence imaging into hospital acquire infections, which is a polymerisation, are temperature sensitive, will go beyond just identifying the location of frightening statistic. Dr Hui Peng from our leading the polymer chains to respectively disease. laboratory has assembled a team to tackle this collapse and become solubilised at higher and problem,” Professor Whittaker said. lower temperatures in water. “We are developing new technologies that will report on the local biology of a patient by The Whittaker Group has a separate research sensing the presence of specific proteins, or program focussed on the development of next “Responsive polymers are measuring changes in ionic make-up or local generation lithography for use in integrated attracting significant interest in temperature. These are all potential indicators circuitry. As integrated circuitry in devices such of tissue health,” Professor Whittaker said. as mobile phones become ever smaller, yet the field for their use in drug and more powerful, technological pressures have gene delivery, tissue engineering Such non-invasive methodologies would been placed on the microfabrication process provide clinicians more comprehensive of lithography, requiring novel solutions to be and in the creation of sensing diagnoses without the need for biopsies, found. devices,” Professor Whittaker which are particularly problematic for internal medicine. The group are developing light-sensitive said. polymers to transfer intricate structures on Professor Whittaker continued his engagement In medical imaging, the group is directing silicon wafers spaced tens of nanometres with China, being the only Australian researcher efforts towards detection and treatment of apart. This is the fundamental process for 2015 to be awarded the Chinese Academy glioblastoma, the most aggressive form of involved in fabrication of integrated circuits. of Sciences President’s International brain cancer with an average life expectancy The group’s experience in polymer science and Fellowship, enabling him to work in Beijing of only 14 months after diagnosis. New photochemistry has attracted collaborations with the National Centre for Nanoscience imaging technologies are being developed with leading global companies such as and Technology. New collaborations were for delineation of diseased tissue in projects Sematech, Intel and The Dow Chemical also forged with Shanghai University, Fudan supported by the NHMRC and the Cure Brain Company. University, and within the framework of Cancer Foundation. the Wuhan-Brisbane Research Alliance in Highlights Functional Polymeric Materials, of which Addressing such complex challenges requires In lithography, the group continues to be Professor Whittaker will direct the Brisbane expertise from a wide range of disciplines. Dr innovators in the field, with an ARC Linkage node. Simon Puttick from the Whittaker Group is Project with The Dow Chemical Company performing pre-clinical imaging, and the group leading to a number of patents being filed in Professor Andrew Whittaker is the Theme is working with molecular biologists to identify 2015, together with licensed technologies. Leader of Imaging Technologies within the ARC molecular targets. Partners with expertise in Funding secured from the Asian Office of Centre of Excellence in Convergent Bio-Nano clinical scanning have also been formed, and Aerospace Research and Development to work Science and Technology.

Whittaker Group www.aibn.uq.edu.au/andrew-whittaker

22 - AIBN Annual Report 2015 Functional genomics drive regenerative medicine

Wolvetang Group www.aibn.uq.edu.au/ernst-wolvetang

Focus The Wolvetang Group is working towards “Generation of isogenic controls through The group had a successful year in solving neurological diseases by understanding CRISPR gene correction is essential for publications, co-authoring a number of the relationships between genes and seeing disease phenotypes emerge at a gene papers in high impact journals, including neurological abnormalities. expression level,” he said. Nature, Science, Nature Communications, Neuron, Human Molecular Genetics, and PLoS Primarily focussed on utilising stem cells The research is also increasingly engineering Genetics. to this end, Professor Ernst Wolvetang said mini organoids from human stem cells with the scope of the laboratory’s work ranges the aim of employing these as 3D models for The group also hosted Cell Reprogramming from common disorders such as Alzheimer’s studying diseases in vitro. The construction of Australia’s 3rd Annual Collaborative Conference disease, to rarer conditions such as forms of these organoides will help develop platforms in May, and Professor Wolvetang was invited childhood leukodystrophy. for screening drugs in a high throughput to present a plenary lecture at the Joint manner, with the postulation that the results Australian- Chinese Academy of Sciences of testing in the cellular environment of an symposium. “We harvest cells from patients organoide will be more relevant than disease and reprogram these into cell types grown in a petri dish. The Wolvetang laboratory was induced pluripotent stem cells, Highlights also part of a new $1 million essentially bringing them back The group built on its strong program in ataxia initiative with Bioplatforms to a fetal state, which then telangiectasia (A-T), which is a rare disease where sufferers lose motor control as a result Australia, led by the Wells allows them to make all the of brain cell degeneration. Affecting around Group, to establish a new stem different cell types in the body,” 50 people in Australia, primarily children, most cells data set. Professor Wolvetang said. patients lose their lives before the age of 20. “We are working very closely with a patient and Professor Wolvetang’s vision for stem cell The group is particularly interested in the parent support group, BrAshA-T, and we are based regenerative medicine of the 21st complex interconnectivity of cells in the brain very grateful for the generous philanthropic century is exemplified by his engagement with and how a breakdown in these connections funding they have provided for our research,” patient advocate groups such as the Mission contributes to disease. They use high content Professor Wolvetang said. Massimo Foundation, which aims to eradicate real time imaging, gene expression analysis childhood leukoencephalopathies. tools, and a range of cell biology techniques in As a potential prelude to cellular therapy for order to identify disease phenotypes. this disease, the group is researching how to “It is at this interface between cutting edge deliver gene-corrected, patient-specific cells research, patient advocacy, philanthropy, The group is currently screening for genes that to the brain of a mouse and determine if they and industry engagement that our stem cell cause demyelinating diseases, where damage integrate and function correctly. Once this research can make a real difference for children to the insulation of nerve fibres in the brain capability becomes clinically viable they hope suffering from rare and currently incurable leads to impaired nerve impulses and to be able to apply this cell-based therapy to diseases,” Professor Wolvetang said. neurological defects. Using clustered regularly children with A-T and other degenerative brain interspaced short palindromic repeats (CRISPR) diseases. technology, the group can mutate or correct genes suspected to be involved in a disease.

AIBN Annual Report 2015 - 23 Naranop ticles to Boost Healthcare Focus The Xu Group is developing nanoparticles to with the PD1-PDL1 interaction which acts as a A collaboration established with UQ’s act as carrier devices for novel applications in shield to mask the presence of cancer from the Queensland Alliance for Agriculture and healthcare and agriculture. body, and provide the immune system’s T cells Food Innovation is investigating oral delivery a target to engage. methods for animals such as chicken or fish Associate Professor Zhi Ping (Gordon) Xu that are farmed in vast numbers. said a significant reduction in the debilitating The other focus of the research group is side-effects of anti-cancer drugs is one of studying how to use nanoparticles to reduce “There can be tens or hundreds of thousands the group’s primary goals in the fight against the disease susceptibility of plants and of animals, so practically you cannot vaccinate cancers. In parallel, the group is also looking to animals. This work is investigating methods to them individually. However, you could quite develop improved cancer treatments that boost deliver therapeutics such as an anti-oxidant, easily deliver a vaccine through food that is the body’s immune system against cancer cells. anti-bacterial agent, vaccine, or gene. then absorbed through the intestines.”

“The agricultural sector suffers from huge “Many anti-cancer drugs in loses each year due to various diseases. Our The team is also using the market are quite efficient aim is to deliver therapeutics and protect environmentally friendly dsRNA animals and plants from infections, insects, in killing cancer cells, however microbes and viruses.” biological pesticides instead of they are very efficient in killing traditional chemical alternatives healthy cells as well, which is Highlights to protect plants and crops from The group was highly productive throughout why chemotherapy is such the year with the publication of more than 15 diseases. journal articles. an awful process,” Associate A limitation of dsRNA pesticides is that they Professor Zhi Ping Xu said. A study appearing in the Journal of Materials quickly break down under natural conditions, Chemistry B provided in vitro demonstration so are only effective for a short period of The team is investigating the delivery of two of lipid coated calcium phosphate (LCP) time. However, when they are loaded into or more therapeutics in one nanoparticle to nanoparticles delivering the functional cell nanoparticles, their release is controlled, produce a synergic effect and minimise the death siRNA to human breast cancer cell line resulting in a longer protection period. amount of therapeutics delivered. Smaller MDA-MB-468. The LCP nanoparticles were dosages reduce the cost of the drugs required, also more effective in inhibiting the tumour In 2015 the group’s work also received a but moreover there is a reduced impact of the growth in comparison with a commercial $100,000 private donation from China for its therapeutic escaping from the cancer target transfection agent. cancer research, following a separate donation and causing cellular damage to other parts of from China in 2014. The group also received an the body. Further work published in this same journal Australian Government Department of Industry showed inorganic layered double hydroxide and Science Research Connections grant with The group is also looking to build on (LDH) nanoparticles pre-coated with albumin Cobbett Pty Ltd, and contract research with these advances in tandem by developing are able to achieve colloidal stability. The NuFarm Ltd for plant protection. nanoparticles that maximise the body’s capacity of particles to resist aggregation is an immune response to fight the illness. Studies important attribute for the successful in vivo are underway using nanoparticles to interfere delivery of a drug or gene.

Xu Group www.aibn.uq.edu.au/zhi-ping-xu

24 - AIBN Annual Report 2015 Applied functional materials Focus The Yu Group is internationally recognised for its development of functional materials that are tailored to needs-based research outcomes in sustainable agriculture, water treatment, energy storage and biomedical applications.

Professor Chengzhong (Michael) Yu said the group’s work combines its knowledge of fundamental material principles to rapidly deliver tailored platforms and systems in extensive collaboration with researchers and industrial partners.

“Nanoscale platforms have a remarkable ability to alter the environments they are in, creating changes that are seen on a much larger scale,” Professor Yu said.

Functional materials can react to their surroundings through catalytic reactions, separate molecules based on size and composition, and have desirable attributes such as solubility or strength.

“By knowing the needs of Queensland, Australia, and the world more broadly, the group is dedicated to needs-driven applications, using innovative functional nanomaterials,” Professor Yu said.

Yu Group www.aibn.uq.edu.au/michael-yu

Highlights 2015 was a successful year for the Yu Group, currently have widespread use in the Australian be synthesised with a large core-cone cavity securing an ARC Linkage Project grant with livestock industry. structure, that is suitable for protein delivery. industry partner Elanco Animal Health, together The findings displayed the highest ever report- with Associate Professor Neena Mitter from The group published a number of influential ed pore size and volume for mesoporous silica UQ’s Queensland Alliance for Agriculture and papers during the year, including a journal nanoparticles with a radial pore structure. Food Innovation. The project, “Novel Na- paper in ACS Central Science as a cover story, no-Pesticides for Animal Healthcare”, will use which investigated mesoporous hollow silica A Chemical Communications paper highlighted a nanotechnology patented during the year to nanoparticles with hydrophilic compositions synthesised mesoporous nanomaterials with develop a new generation nano-pesticide with and hydrophobic properties as nano-carriers co-modified aptamers and methyl groups, improved safety and performance. for diverse bio-applications. Findings in the which provide more sensitive and measurea- paper showed that nanoparticles with a rough ble detection of insulin in serum compared to surface displayed an enhanced antibacterial commercially available products. “Spinosad, a naturally derived effect when loaded with antibiotic vancomy- pesticide with low environmen- cin, compared to nanoparticles with a smooth Professor Yu co-chaired the 9th International surface. Mesostructured Materials Symposium (IMMS- tal impact and low toxicity, will 9), which was held at the Brisbane Convention be loaded into designer na- Additional work published in Chemistry of Ma- and Exhibition Centre during August. He also terials showed mesostructured hollow carbon received the 2015 Le Févre Memorial Prize for no-carriers which will improve nanoparticles can be synthesised with bi- and Chemistry from the Australian Academy of adhesion to skin and hair and triple-layered walls for diverse biomedical Science. applications. Furthermore, a paper published in protect against UV degradation,” Nanoscale showed mesoporous hollow silica– More broadly in the group, three research Professor Yu said. fullerene nanoparticles can be prepared using a higher degree students completed their selective etching method, and have a signifi- studies during the year, and Dr Jun Zhang was The nano-spinosad pesticide is expected to cantly higher drug loading capacity as well as awarded the 2015 Distinguished International enhance the efficacy and effective duration enhanced photodynamic properties. Students Scholarship by the China Scholarship in field conditions, significantly reducing the Council. cost of pest control and the risks associated Related work was published in Small, which with the highly toxic synthetic pesticides that showed mesoporous silica nanoparticles can

AIBN Annual Report 2015 - 25 associate group leaders

Dr Claudia Vickers Dr Chun-Xia Zhao Associate Professor Dr Zhongfan Jia Queensland Government ARC Future Fellow ARC Future Fellow Accelerate Fellow Kristofer Thurecht ARC Future Fellow Sustainability through the devel- Dr Chun-Xia Zhao’s research pro- Associate Professor Kristofer Thure- Dr Zhongfan Jia is working to un- opment of novel bio-products is a gram is focussed on improving cht is developing new materials in derstand the basic properties of core focus area of Dr Claudia Vick- production of nanomedicines, and nanomedicine that combine the polymers and applying them in ers’ research, which aims to rede- faster screening and evaluation of ability to deliver therapeutics with areas as broad as stem cells and sign microbial cells for producing promising nanomedicine candi- monitoring disease progression. batteries. industrially useful bio-chemicals. dates using biomimetic chips. Holding a joint appointment be- During 2015 he won a UQ Foun- “A lot of the products we are looking Medical research takes many years tween AIBN and UQ’s Centre for dation Research Excellence Award at are replacements for petrochem- to progress. Successful drug candi- Advanced Imaging, he is a polymer to provide one year of funding to icals, to make more sustainable dates first need to be packaged in chemist with a goal of developing develop a battery made entirely of and environmentally friendly chem- a drug delivery system that effec- new materials classed as ‘thera- plastic. icals,” Dr Vickers said. tively transports them to the target nostics’. “It is an exciting new area that cell or organ. This is followed by She focusses on a group of natural “We want to have an approach shows a lot of promise, however, it another five to 10 years to proceed organic compounds called terpe- where the material we design is is only in the earliest stages of de- through animal and then human noids. This group includes com- able to deliver a therapeutic agent, velopment,” Dr Jia said. clinical trials. pounds that can be used as biofu- but also assist in monitoring its ef- The vision sees a fully completed els and rubbers, as well as specialty “A lot of drug delivery systems have fectiveness at targeting particular product where the waste battery chemicals that can be used to im- been developed, however, many fail biomarkers,” Associate Professor could be disposed of into a recy- prove crop yield and make industri- in human trials despite working in Thurecht said. cle bin, without the environmental al fragrances. animal models,” Dr Zhao said. Using tumours in animal models to concerns of heavy metals used in “Making cells do something that “We are interested in developing test the materials, his work utilis- current batteries. they haven’t spent millions of years in vivo mimicking biochips, with es positron emission tomography His work under an existing ARC evolving to do is not trivial,” she critical cell types that resemble (PET) to see therapeutic agents Future Fellowship also saw him said. different human organs onto small effectiveness in three dimensions, utilising polymers in a project that chips, allowing tests to occur very together with magnetic resonance Dr Vickers uses synthetic biology to aims to generate large quantities of quickly, and at lower cost.” imaging (MRI) and optical imaging. create new biological components, undifferentiated stem cells for the or substantially modify existing 2015 was a productive year for Dr In 2015 Associate Professor Thure- first time. components that can be applied Zhao; building collaborations with cht was awarded an ARC Linkage “The applications of polymer ma- to re-engineer cells. She then uses Harvard University and Cornell Uni- Project with AIBN Group Lead- terials are very broad and can be systems biology tools to examine versity, receiving a UQ Advancing er Associate Professor Stephen completely different, but as poly- the effect of her engineering ap- Women Researchers Grant, and Mahler to partner with Minomic mer chemists, our aim is to be able proaches. beginning an ARC Future Fellow- International Ltd on developing to precisely design and synthesise ship. an antibody delivery platform for She also investigates sustainable the polymer materials with desired a prostate cancer antigen. He will feed stocks, particularly sucrose, Additionally, she received an ARC properties for specific applications,” also lead a facility for characteris- which is a large economic driver Discovery Project for further de- he said. ing bionanomaterials through the locally in Queensland. veloping the patented nanocapsule ARC Linkage, Infrastructure, Equip- Work undertaken in his Future Fel- technology and an ARC Linkage Further research applies sys- ment and Facilities scheme. lowship project is using polymer Project grant for improving the tems biology tools to examine the nanostructures that are decorated manufacture of nanoparticle-based An NHMRC Project Grant was 7,000-year-old bioprocess of beer with growth factors to facilitate the adjuvants in partnership with Vax- awarded to him as lead investi- making. She aims to better under- generation of stem cells in a three ine Pty Ltd. gator to study immuno-polymeric stand how each of the three differ- dimensional environment, allowing drugs for prostate cancer therapy. ent organisms that go into making for a new source of stems cells that In further success during the year, beer – barley, hops, and yeast – im- could be applied in regenerative he and Associate Professor Mahler pact its quality. medicine. were awarded a patent for develop- ing new antibody conjugates using antibody engineering combined with imaging.

26 - AIBN Annual Report 2015 High achieving early and mid-career researchers at AIBN are recognised with appointment as Associate Group Leaders. These researchers are the next generation of leaders, selected for their excellence in research output, collaborations, and professional leadership qualities.

Dr Esteban Marcellin Associate Professor Dr Muhammad Dr Simon Corrie Queensland Government ARC DECRA Fellow Accelerate Fellowship Idriss Blakey Shiddiky Principal Research Fellow NHMRC Career Development Fellow Dr Esteban Marcellin’s research Associate Professor Idriss Blakey Dr Muhammad Shiddiky is working Dr Simon Corrie has led research in is focussed on reducing industrial is developing new materials for the on the development of a new gen- the development of a needle-free waste and producing chemicals generation of semiconductors and eration of cancer detecting devices. blood test device, dubbed the Mi- and fuels sustainably. sensors in future technologies. cropatch. Circulating tumour cells are the The highlight of the year was the “We are working with The Dow invasive bodies that enable the This new device, which initially establishment of a gas fermenta- Chemical Company on an ARC spread of cancer by travelling from looks similar to a Nanopatch, fea- tion group and facility hosted at Linkage Project to develop poly- one tissue site to another through tures longer projections for deep- AIBN, which is the first of its kind in mers that improve the manufactur- the bloodstream. As a result, the er entry into the skin and access Australia. The facility was enabled ing processes for semiconductors detection of these cells and diag- into the top layer of blood vessels through a UQ Major Equipment and to be used in computer chips such nosis of the particular cancer type known as capillaries. Infrastructure grant, and an ARC as random access memory,” Asso- is vital to inform therapeutic strat- “We’re really excited that we have Linkage Project with Lanzatech. ciate Professor Blakey said. egies. now progressed to testing this Concurrently, he worked with The new diagnostic microneedle array Dow Centre for Sustainable Engi- The project has been highly suc- “We are trying to develop devices in a dengue fever disease model; neering and Innovation to evaluate cessful, with the filing of three pat- for circulating tumour cell detec- previously we have been restricted the economic feasibility of ferment- ent applications during 2015 based tion using microfluidic devices and to testing artificial biomarkers,” Dr ing methane. on technology developed during electrochemical nanobiosensors,” the project. A further patent was Dr Shiddiky said. Corrie said. “There is a lot of gas that is flared also granted for a previous project It is hoped that microstructured The new patch selectively collects and lost, which is a major contribu- with Intel. biomarkers from the blood in the tor to climate change,” Dr Marcellin electrodes may enable this diag- skin, which can then be analysed said. In the same area of photolithogra- nosis by selectively capturing bi- phy, he is working on an ARC Dis- ological data, achieving not just offline. “Finding a method for recapturing covery Project with Dr Kevin Jack increased sensitivity but the ability Dr Corrie has worked together with carbon monoxide, carbon dioxide from UQ’s Centre for Microscopy to achieve a diagnosis in a quicker the Australian National Fabrication and methane, and converting them and Microanalysis. fashion than current methods. Facility – Queensland at AIBN to into fuels and chemicals would pro- develop a microfluidic device that vide a new way for reducing climate “We are looking to develop self-as- “Our work with biomarkers to de- will make the Micropatch a stan- change through biotechnology.” sembling polymers, using light to velop diagnostic devices could also direct the nanoscale structure of be applied to other diseases out- dalone diagnostic tool without the He is also working with The Dow the polymer.” side of cancer,” he said. need for a laboratory. Chemical Company to sustainably “This would make it a very practi- produce propionic acid from sugar A research paper based on the Dis- This research, initially funded cal field device, especially for areas – a collaboration that won him the covery Project was published dur- through an ARC Discovery Project, where hospital and laboratory ac- 2015 Science Prize of the UQ Part- ing the year in Macromolecules. has resulted in the most produc- tive couple of years in Dr Shiddiky’s cess is limited, allowing for a quick ners in Research Excellence Award. Research partnered with the career with more than 20 research diagnosis,” he said. Queensland University of Technol- “A bacterial strain we found papers published. The work is supported by an NHM- achieved our target yield, and it is ogy is studying the design of a new RC Development Grant, ARC Dis- in the process of being patented,” type of sensor. Unlike today’s de- In this time he has also obtained a covery Early Career Researcher he said. vices that rely on electronic sens- NHMRC Career Development Fel- ing, is has a wider sensing range lowship, and developed closer ties Award, and the ARC Centre of Ex- Further success saw another ARC and detects chemical signals using with the University of Washington. cellence in Convergent Bio-Nano Linkage Project awarded with in- light. Science and Technology. dustry partner Zoetis Australia. This will support research aimed at low- ering the cost of producing clostrid- ia toxoid vaccines, commonly used in livestock, by decreasing the high level of batch failures and improv- ing toxin production yields.

AIBN Annual Report 2015 - 27 Discoveries AND collaborations

28 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 29 MERS antibody building on Hendra virus work

One of the Institute’s most outstanding that would impact its suitability as a drug MERS has been linked to dromedary camels achievements has been the successful candidate to treat infected patients. as the reservoir for the coronavirus, and there partnership between AIBN and Queensland are estimated to be approximately 300,000 Health to produce an antibody treatment for The antibody was initially discovered by a team of the animals in Australia, which were first the deadly Hendra virus. of US researchers led by Professor Christopher introduced in the 1840s. Broder at the Uniformed Services University of 2015 was a landmark year for this collaborative the Health Sciences, and Dr Dimiter Dimitrov Given previous experience that the Hendra endeavour, with the m102.4 antibody entering of the National Cancer Institute, National virus jumps from bats to horses, and phase 1 clinical safety trials run by Q-Pharm in Institutes of Health. from horses to humans, the Queensland Brisbane. Government has prioritised the need to identify AIBN advanced this discovery to successfully a means to help combat the MERS virus. Professor Peter Gray said there was good develop the capability to produce the antibody cause to be optimistic about the trial. at the highest quality and in sufficient The same team of US researchers has now quantities for the clinical trial to occur, as well developed an antibody for use in MERS, leading “The Hendra virus has claimed as provide emergency supplies of the antibody to high level discussions in Washington with for further compassionate use if required. the Queensland Chief Health Officer to bring the lives of four people since the antibody to Australia. 1994, and the availability Professor Gray said the threat of another virus, Middle East respiratory syndrome (MERS), led and use of the antibody on the parties to discuss the continuation of the “AIBN has been at the centre compassionate grounds means collaboration in a new area. of these discussions, given that there is now a treatment “Since its discovery in 2012, MERS has claimed our mutual track record in the to offer people who are at risk the lives of more than 400 people in more than production of the Hendra virus 20 countries,” he said. from the disease.” Professor antibody,” Professor Gray said. “Just one infected patient entering South Gray said. “We are hopeful that a similar agreement can Korea resulted in thousands of people entering be reached to help secure our biosecurity.” The clinical safety trial is aimed at better quarantine and the deaths of 36 people, which understanding the impact of m102.4 on the shows that MERS is a highly virulent disease, body, and whether it has adverse side effects and has made Queensland Health take notice.”

30 - AIBN Annual Report 2015 CHEAPER, CLEANER PRODUCTION OF CARBON FIBRE

AIBN research from the Martin Group has “We discovered that if we blend the The team has signed a landmark agreement developed an innovative technology that polyacrylonitrile with only a small amount of with the Myuma Dugalunji Aboriginal promises to radically disrupt the global carbon our spinifex-additive, then we can reduce the Corporation in Camooweal, north-west industry by producing a more cost-effective furnace temperature – which is a large energy Queensland. It provides a mechanism to and sustainable product. saving,” Professor Martin said. commercialise the technology with indigenous- royalty sharing in any commercialised products Current production methods for the lightweight Even more incredibly, the team has discovered that evolve out of the technology. and strong material use unsustainable that the new nanocellulose material can be petrochemical precursors, and are costly cleverly used to enable further significant “The grass grows where to manufacture. This makes the material reductions in PAN precursor cost. economically unfeasible for many industries indigenous communities are, that would otherwise benefit from its “We’ve experimented with blending our and if we can develop this advantages. nanocellulose with the PAN precursor in all sorts of ways, and some of them happen to process then this opens up Existing production techniques use a precursor be dramatically more cost-effective, we now strong economic possibilities polymer resin called polyacrylonitrile, or PAN, have to work with our collaborators at Deakin which is spun into threads finer than a human University to take this proof-of-concept and across regional Australia,” hair, and then fired in a furnace at increasing scale it up over the next two years,” he said. Professor Martin said. temperatures to convert it into strong, stiff Such breakthroughs would revolutionise the carbon fibre. The research is also supported by an ARC carbon fibre industry if successfully scaled up, Discovery Project in collaboration with Carbon however, Professor Martin said the team have “You don’t need the world’s strongest or stiffest Nexus at Deakin University, providing access an even more ambitious goal in mind. carbon fibre to make a wind turbine blade, to state-of-the art carbon fibre production and for instance. It just needs to be lightweight testing facilities. and lower cost than, for example, heavier but “The Holy Gail of carbon fibre research is to remove the solvents used to make it, which currently cheaper fibreglass – and that is “Commercially there is a black art to making aren’t particularly green.” where our technology is targeted,” Professor carbon fibre, and the big players do not share Darren Martin said. information, so we’re looking at opening up The nanofibres the team have created can that knowledge with Carbon Nexus at Deakin suspend in water and are long and thin, lending To improve the process, the group is using University,” he said. spinifex grass – a native grass which is themselves towards the spinning and aligning required for thread production. This could endemic in outback Australia – to develop Research on spinifex-derived nanocellulose result in a totally water-based textile that nanocellulose, which they have demonstrated was published in Cellulose and RSC Advances removes the solvents from the process. to have remarkable properties. during the year.

AIBN Annual Report 2015 - 31 Getting biomarkers out of the skin with Micropatches

The Kendall Group is researching a device, “The obvious advantage is that the Micropatch can access biomarkers in blood vessels, which rather than delivering vaccine to the skin, is far less invasive than a needle. But potentially instantaneously and over several hours as a instead takes biological samples away as a of even greater relevance, we envisage that as wearable device. needle-free blood test. a diagnostic tool the Micropatch would only require minimal training and would offer an First author Dr Jacob Coffey said, “The Professor Mark Kendall said the Micropatch immediate diagnosis,” he said. Micropatch is designed with different features projections designed for selectively- projection shapes to sample material, with extracting biomarkers from the outer layers of “Currently patients have to wait surface chemistry to allow for the selective the skin, rather than needing a needle or lancet extraction of biomarkers.” for a blood sample. for a diagnosis, especially in developing countries; whereas The projections featured on the Micropatch “By applying the Micropatch to the skin, and are also longer than those on the Nanopatch, leaving it on for under an hour, the micro an immediate diagnosis allows due to the need to access deeper into the skin. projections are exposed to the fluid in the treatment to begin straight Despite this, the Micropatch only delves as far skin – which includes blood in the capillaries as the capillary loops in the dermal papillae – – capturing protein biomarkers which can away.” or fingerprints – for target probes to capture be analysed when the patch is removed,” target biomarkers. Professor Kendall said. Kendall Group PhD student Kye Robinson was the first author on a paper published in In 2011, Professor Kendall founded the “The device is being advanced to have ‘plug- Biointerphases, and said the Micropatch has key UQ start-up Vaxxas, which achieved initial and-play’ functionality with a diagnostic reader. differences from the Nanopatch. capital raising of $15 million to advance the Achieving this will allow for quick and easy Nanopatch through clinical testing and along diagnosis of diseases such as dengue fever or Proof of concept was successfully established the pathway to a commercial product. A further malaria.” during the study, with in vivo capture of $25 million was secured during 2015 through antibodies specific to the dengue NS1 protein Series B venture financing, funding Nanopatch Professor Kendall said that while blood from the skin of a live mouse model. clinical programs and the application of further samples taken with a needle and syringe are vaccines. effective, the Micropatch offers a number of This was followed by work published in competitive advantages. Biomaterials showing how Micropatches

32 - AIBN Annual Report 2015 High performance cluster boosts research capabilities

Computational capabilities at UQ were This increase in processing speed is “As an example of what we work on, the cluster significantly boosted in 2015 with the arrival of attributable to an increase in the number enables us to rapidly model different materials a new high performance computing cluster at of cores from 280 to 500, and a significant and the impact of manipulating various AIBN. increase in memory per core. Critically, components of these materials,” Dr Hankel however, half of the new cores come from said. A UQ Major Equipment and Infrastructure grant the new Phi co-processor family, which can awarded to Professor Debra Bernhardt enabled operate numerous data threads similar to a the purchase of the $275,000 cluster dubbed GPU (graphics processing unit). “That allows us to model ‘Golden Orb’. materials that have different “The Phi cores are used to give the calculation Replacing the previous ‘Funnel Web’ cluster, a boost, by providing extra cores that are chemical elements added to which operated for six years, the new cluster located on the same node and do not need to them, and we are able to model became operational in April to the immediate be reached over the network,” she said. benefit of computational researchers. how that changes the material’s “We are seeing a better speed up for a behaviour,” she said. Dr Marlies Hankel of the Bernhardt Group calculation using the Phi cores compared to a manages the new equipment, which has calculation using a second node. The new Golden Orb cluster has a modular significantly accelerated the time it takes to run design which will allow future expansion to modelling simulations. “The other big advantage of the Phi cards is meet the growing demand for state-of-the-art that existing programming languages can be computational capability. used and programs do not need to be rewritten, “Runtimes are much quicker; like for a GPU.” in a day we can now run a In addition to the Bernhardt Group, the calculation that would have advanced capability of the new cluster is being taken a week with the old utilised by the Wells, Middelberg, Yu and Wang Groups at AIBN, as well as a number of others cluster,” Dr Hankel said. across the University, including the Centre for Hypersonics.

AIBN Annual Report 2015 - 33 Self-assembled nanocapsules deliver results

Nanocapsule technology invented by Professor High temperatures or extreme pH levels with Although highly effective as a pesticide, Anton Middelberg and Drs Chun-Xia Zhao solvents are then used to remove the core, fipronil has been identified as one of the and David Wibowo could be applied to a leaving the shell. leading causes behind the declining number variety of industrial applications, with a lower of bee colonies worldwide, and also affects environmental impact during production and “These are difficult steps, and a lot can go many other animals including insects and usage. wrong – the shell can rupture, or the agents fish. Consequently, discovering a method that are meant to be added might be too big for of minimising its consequences is of high Group Leader Professor Anton Middelberg said the gap that is created,” Dr Zhao said. importance. UQ has developed a patented process of using a peptide or a protein to self-assemble a shell Nanoparticles have been studied worldwide “The termite pesticide fipronil is highly toxic around a pharmaceutical-grade oil that can as delivery vehicles for many years, however to the environment, and current methods rely carry a chemical or therapeutic cargo. these manufacturing processes have proven on burst release of a large amount of it at limiting for bringing the technologies to some once. However, using fipronil in conjunction “What’s unique about the markets. with the nanocapsules resulted in a significant materials saving and lower environmental nanocapsule technology is that “However, our system is very simple. The impact,” Dr Zhao said. you make the droplet at room proteins and peptide sequence at the interface harden into a silica shell that surrounds the oil temperature with the proteins droplet, which is pre-loaded with the agent you Research on the termite field on the surface, which self- want to deliver,” she said. trial was published during the assemble, and when a reagent This method has the additional advantage year in Langmuir, and a new is added a protective silica layer of having a high loading capacity, and the trial began in December 2015 precursor can control the shell’s thickness, builds around the surface,” he which in turn controls the speed of release. looking to eliminate termite said. Together with agricultural applications, the nests within a period of several nanocapsules could also be used in areas as weeks. The research program “At no point do you use any toxic solvents or diverse as health and cosmetic products. high temperature in the process, whereas the is funded through an ARC traditional way is very resource and energy As a proof of concept, the team collaborated Discovery Project grant. intensive.” with Mr Brenton Peters, formerly Principal Entomologist at the Queensland Department Associate Group Leader and ARC Future Fellow of Employment, Economic Development Dr Chun-Xia Zhao said that the traditional and Innovation, in a field trial to use the approach of producing nanoparticle carriers nanocapsules as a cost-effective termite uses what is called a template particle, which a pesticide with a lower ecological impact than layer of silica is grown around. when used with current delivery methods.

34 - AIBN Annual Report 2015 Fast, cheap and easy to use tuberculosis screening

The dire need for improved diagnosis of tuberculosis (TB) has led UQ researchers to develop a testing platform to suit the specific needs of developing countries where the illness is most prevalent.

Work led by AIBN’s Professor Matt Trau and Dr Nick West of the School of Chemistry and Molecular Biosciences is looking to build a system that is substantially cheaper and easier to use than those currently available.

The work is based on the “Single Drop Genomics” technology first invented through a collaboration between Professor Jimmy Botella’s laboratory at UQ’s School of Agriculture and Food Science and the Trau laboratory.

“Though the numbers are slowly dropping, around 10 million people still fall ill from TB each year, and devastatingly this is fatal for around one and half million of these people,” Professor Matt Trau said.

“Left unchecked, tuberculosis is an extremely powerful illness that together with HIV is one of the leading causes of death worldwide, making the need for an easily accessible diagnosis even more pronounced.

“In developing countries there is an urgent need for a way to diagnose people cheaply, and in a way that requires minimal training as medical practitioners are thinly spread on the ground.” “We are also incorporating isothermal DNA If successful, this research is of immense amplification, which allows us to work at a significance. The potential cost savings Collaborator Dr Nick West said many existing constant temperature to copy the DNA and per test will be amplified many fold when detection methods have limitations including increase the signal without the need for extrapolated across the millions of diagnostic cost or lengthy wait times for accurate results. sophisticated laboratory equipment.” tests that are performed annually on suspected This presents problems for wider adoption in TB cases worldwide. developing countries at the point of care. In 2016 the team will test the system with patient samples, and hopes to have a workable This research has been “PCR (polymerase chain reaction) tests prototype for field trials in 2017. published in three seminal exist, which are accurate and quite quick, but papers in 2015, with findings they are expensive and require training and “On the patient side we would ultimately like expertise to run and interpret,” he said. the test to be available in the local pharmacy appearing in ACS Sensors, for a cheap price,” Mr Ng said. Scientific Reports, and “There are also other methods to detect TB, such as a skin test, but if you have previously “A person feeling unwell at home could Analytical Chemistry. been inoculated with the BCG vaccine, which purchase the test, give a sample, run the test, is the only vaccine against TB currently used, and within the hour know if they are infected The work is in part funded by a donation of then there is a high chance you will return a with TB. more than $3 million to UQ in 2012 by the TB positive result.” Sailors’, Soldiers’ & Airmen’s Association of “Once you identify a person with active TB, you Queensland, and continues the University’s PhD student Mr Benjamin Ng said their test can administer antibiotics immediately and historical connection with the illness. UQ’s analyses DNA biomarkers from tuberculosis for take measures to ensure they do not spread Fryer Library is named after a former UQ a reliable result. the illness further, and you can also quickly student and First World War veteran, John and cheaply screen people they’ve already Denis Fryer, who died of tuberculosis in 1923. “Sputum is the easiest way to obtain a sample been in contact with. This will bolster national from a patient with TB, but often they also healthcare efforts to control the spread of cough up blood which tempers the sample, TB through systematic screening and early which means that our system requires a robust diagnosis.” DNA purification technique,” Mr Ng said.

AIBN Annual Report 2015 - 35 Industrial Affiliates Program removes barriers between academia and industry The Industrial Affiliates Program (IAP) is a conduit between AIBN’s ground-breaking research and the establishment of industry partnerships.

Founded in 2009, the IAP directly links domestic and international industry with the Institute via a membership program, together with networking events held throughout the year.

The program enables the formation of partnerships to accelerate research and form consultative agreements.

Networking events There were three very well attended IAP events during the year, beginning with the annual Showcase and Networking Event held at AIBN on 19 March.

More than 60 guests from industry, philanthropy, academia and government were given a taste of the research undertaken at the Institute.

Professor Andrew Whittaker spoke about advances in battling brain cancer, while Professor Peter Gray described new technology for growing stem cells for regenerative medicine.

Professor Matt Trau closed the evening with a stimulating discussion about his research aimed at developing a novel diagnostic platform that will enable personalised nanomedicine with the capacity to tailor treatments to patients based on their individual physiology and disease pathology.

On 16 April the eighth event in the AIBN The year’s final event was the ninth AIBN Membership Thought Leaders’ dinner series was held at Thought Leaders’ dinner held on 1 October at The IAP provides three levels of membership, Customs House, with guest speaker Professor Customs House. with scaled levels of access to AIBN Jim Patrick AO describing his journey in researchers and facilities. developing the globally successful cochlear Dr Alan Finkel AO, then Chancellor of Monash implant technology. University and President of the Australian More than 20 companies have joined the Academy of Technological Sciences and program which, at its highest level, provides His presentation included Engineering, spoke about his experience in significant discounts on consulting fees and founding the US-based, ASX-listed company privileged access to AIBN researchers to personal insights into Axon Instruments, from original idea through engage with the member company’s own to acquisition by NASDAQ-listed Molecular personnel. biomedical research and Devices. He shared what he considered to engineering innovation, as well be the key drivers behind this successful AIBN Industry Fellow Dr Ian Nisbet said IAP as education and professional venture, gave thought-provoking insight on the memberships provide affordable access to innovation culture in Australia, and challenged companies of differing sizes and needs. associations. The cochlear us to change the way we see ourselves by “Companies are fortunate to be able to success story was particularly acknowledging and celebrating our global work with our researchers for their own success stories. separate programs, together with developing relevant to those in attendance, relationships that could lead to joint- with the development and developments,” Dr Nisbet said. commercialisation of novel “The Premier, Member, and Associate Member technologies at the forefront of levels of the program provide tailored access depending on the company’s needs and AIBN’s mission. budget.”

More information: www.aibn.uq.edu.au/iap.

36 - AIBN Annual Report 2015 AIBN Early-/Mid-Career Support Program

Following its formation in 2014, the Early-Mid Program in collaboration with the AIBN grants AIBN Group Leaders rotated their attendance at Career Researcher (EMCR) Committee enjoyed unit and executive. This encompasses a range the EMCR Committee meetings, offering valued a successful year of expanding its program of support initiatives targeted at improving support and guidance in developing the many of support and educational initiatives to the the scientific and written quality of grant activities throughout the year. strong cohort of EMCR researchers within the applications, and also educating EMCRs on Institute. the various schemes and how to best position The committee is seeking to expand its themselves to be competitive. The program activities to develop initiatives that support AIBN EMCR Chair Dr Michael Crichton said culminates in a pitch session to a panel of EMCRs in achieving work/life balance, EMCRs, which comprise researchers in the first scientific experts where EMCRs have the particularly focussing on ways to build five to 10 years of their career after completing opportunity to rigorously test the merit of their a research career while balancing family a PhD, face the challenge of establishing their application. commitments. career after an extended period of education. Following a survey of the EMCR cohort, “Most EMCRs are of an age where starting a “It’s not a situation too dissimilar to what any the committee initiated a professional family is important, and they do not want to other university graduate faces as they enter development seminar series during the year. miss out on having a career or a family,” he the workforce and try to establish a career,” Dr said. Crichton said. “We ran seminars to inform the cohort about commercial avenues for research; what Members of the committee also assisted “We look to support the 100 or kinds of work are of interest to industry, and in hosting the 6th Annual Early Career developing career paths for researchers Researcher Symposium, which was held at UQ so EMCRs at AIBN through a outside of academia,” Dr Crichton said. in December and attended by more than 200 number of initiatives that assist registered guests. Over the course of the year the committee them on a professional and ran three networking events at AIBN to foster In 2016 the committee will continue building personal level.” internal collaborations and discuss the EMCR its program, collaborating with other institute environment. The committee also worked committees and engaging with EMCRs. closely with other UQ institutes to cross- Dr Ilaria Stefani will take over as Chair the Faced with a competitive funding environment, promote events for EMCRs. committee, with a team of enthusiastic EMCRs. the EMCR Committee launched the GrantReady

AIBN Annual Report 2015 - 37 Funding and recognition L-R: Professor Mark Kendall, The Hon. Christopher Pyne - Minister for Education and Training, The Hon. Jane Prentice - Federal Member for Ryan, Professor Peter Gray, and Professor Peter Høj.

38 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 39 Aanustrali Research Council funding

Over the course of 2015, AIBN researchers $400 million loss of Australian livestock to ticks refrigeration but is advantageously stable at were involved in Australian Research Council and the buffalo fly. A $330,000 grant will study room temperature in a $430,000 project. grants that totalled more than $5.8 million. the use of a naturally derived pesticide called Spinosad in silica hollow nanospheres for Professor Matt Trau secured $315,000 to The Institute’s commercial focus was reflected improved adhesion to fur and skin, and reduced develop a technology that can capture and in particularly strong results in securing ARC ultraviolet degradation. study nano-sized molecular complexes in their Linkage Projects, which provides funding to natural state for use in biology, biotechnology partner with external companies and end- Professor Kirill Alexandrov will lead the study and advanced materials. user organisations to expedite translation of of novel peptides for use in pharmaceuticals innovative ideas and technologies. in a $670,243 grant partnered with Phylogica Professor Lianzhou Wang will develop Limited. photocatalyst systems that can convert waste products into valuable chemicals using solar In total, AIBN researchers were In further research involving AIBN researchers, energy in a $310,000 project. Professor Kirill named investigators on six new Professor Lianzhou Wang will partner with Alexandrov will also lead a $650,000 project to Professor Han Huang of the UQ School of develop electric biosensors that utilise a new ARC Linkage Project grants, Mechanical and Mining Engineering and class of artificial protein receptors. attracting funding in excess of Baosteel Company in a $377,000 project to study the development of water‐based nano‐ Dr Yu Chen from the Wang Group received a $2.3 million. additive lubricants for use in steel production. prestigious Discovery Early Career Researcher They aim to improve the quality of steel and Award worth $355,000 for the development of $488,000 was awarded to Professor Lars reduce waste by-products. a diagnostic and therapeutic nano-platform for Nielsen and Dr Esteban Marcellin for research cancer treatment. to lower the cost of producing toxoid vaccines Professor Darren Martin was named on a commonly used in livestock. Partnering with $159,661 Linkage Project with DOW Chemical Separately, Associate Professor Kristofer Zoetis Australia, they will look to decrease the (Australia) Ltd to develop innovative, high Thurecht will lead the establishment of an high level of batch failures and improve vaccine performance carbon fibres that are cost‐ advanced facility for the characterisation of concentration. effective using plastic polyethylene to reduce bio-nanomaterials in biomedical applications, the restrictive price of carbon fibres. with the award of a $650,300 Linkage Associate Professors Kris Thurecht and Steve Infrastructure, Equipment and Facilities project. Mahler will partner with Minomic International The Institute also secured four ARC Discovery Ltd on a $360,000 grant to develop a stable Projects, a prestigious Discovery Early In partnership with Professor David Williams nanomaterials and biomolecules delivery Career Researcher Award, and two Linkage of the UQ School of Civil Engineering, platform for antibodies, using promising Infrastructure, Equipment and Facilities grants. Professor Darren Martin was an investigator prostate cancer antigen MIL38 as a on a successful Linkage Infrastructure, translational test bed. Associate Professor Linda Lua and Professor Equipment and Facilities bid for $800,000 to Anton Middelberg will study a single-dose create a national rock, concrete and advanced Professor Chengzong (Michael) Yu and Elanco nanovaccine for cost-effective influenza composite testing facility. Animal Health will look to combat the annual poultry vaccination, which does not require

40 - AIBN Annual Report 2015 Naation l Health and Medical Research Council

AIBN celebrated a very successful year in “We’re developing a targeted approach that In a joint project with UQ’s Professor Maree National Health and Medical Research Council uses nanomedicine to increase the delivery of Smith from the School of Pharmacy, Professor funding, attracting new project funding totalling the therapeutic to the tumour site, and reduce Andrew Whittaker was awarded $796,950 to more than $4.5 million. the amount lost to other sites,” Associate study relief strategies for patients suffering Professor Thurecht said. from advanced cancer by using polymer microparticles. The Institute achieved an AIBN Group Leader Professor Justin Cooper- outstanding 60 per cent White secured $618,493 of Project Grant “We are looking to develop polymer funding to study the direct reprogramming of microparticles that can be injected into spinal success rate in AIBN-led adult cardiac fibroblasts following injury. fluid, and deliver a prolonged-release of NHMRC Project Grants – analgesic drugs with the objective of reduced “Rather than a patient’s heart tissue becoming complications and longer periods of pain relief,” significantly higher than the scarred, it could become functional heart Professor Whittaker said. national success rate of 13.7 tissue,” Professor Cooper-White said. A $621,979 project in collaboration with Dr per cent. A total of six new “Current drug-based therapies work towards Kate Stacey and Professor Paul Young from projects will commence in 2016, stopping further damage to the heart, but the UQ’s School of Chemistry and Molecular impact on heart repair is marginal at best.” Biosciences will see AIBN’s Dr David Muller in addition to two new NHMRC study the dengue virus NS1 protein as a Development Grants which A novel therapeutic strategy for metastatic mediator of pathology. melanoma will be the subject of a $546,495 support research at the pre- study by AIBN’s Dr Barbara Rolfe. The research Professor Kirill Alexandrov, who shares a joint seed stage to drive commercial will investigate how an immune protein, appointment between AIBN and the Institute C3a, regulates melanoma metastasis – an for Molecular Bioscience, was awarded a outcomes within a five-year aggressive and advanced form of skin cancer $587,360 NHMRC Development Grant for work timeframe. that has spread to other sites. on developing a point-of-care diagnostic test for immunosuppressant drugs. AIBN Associate Group Leader Associate “We have seen dramatic reductions in the size Professor Kristofer Thurecht will lead a project of primary tumours with this treatment in the Professor Peter Gray was named on a studying immuno-polymeric drugs for prostate laboratory, and will now look into whether it can $736,300 Development Grant administered by cancer therapy. The $626,995 study, which also also reduce the spread of the metastasis,” Dr the University of Sydney and led by Professor involves AIBN’s Associate Professor Stephen Rolfe said. Derek Hart for the a new immunosuppressive Mahler and Dr Christopher Howard, seeks to monoclonal antibody to advance eliminate side-effects of chemotherapy agents. transplantation.

AIBN Annual Report 2015 - 41 Fellowships fuel research opportunities

Research fellowships are awarded through Professor Christine Wells was recognised for Dr Yu Chen of the Wang Group will study the highly competitive funding schemes and her leading work in understanding stem cells development of two-dimensional nanosheets provide support for researchers recognised for with the award of one of only fifty ARC Future for novel applications under an ARC Discovery their exceptional talent. Fellowships awarded nationally. Early Career Researcher Award.

Her work will seek to understand the systems The nontoxic and biocompatible manganese- During 2015 the strength biology of stem cells and how complex oxide nanosheets have the potential to perform of innovation and output populations of stem cells behave. as a new theranostic nanomaterial platform.

demonstrated by AIBN It is hoped that identifying new classifiers for Dr Chen will investigate their use as stimuli- researchers resulted in another stem cells will provide molecular predictors responsive agents that could combine for the differentiation potential that stem cells simultaneous targeting, magnetic resonance successful year of achieving have, which control what type of cell they will imaging enhancements, and drug release and fellowship funding. become. delivery.

Professor Lars Nielsen was awarded a highly The project will use new bioinformatic methods Internationally, Professor Andrew Whittaker prestigious Novo Nordisk Foundation Laureate and develop statistical models that address was the only Australian during the year to Research Grant which will fund his research to population heterogeneity for new insights into be awarded a Chinese Academy of Sciences the value of $8.6 million over seven years. stem cell biology and tissue engineering. President’s International Fellowship Initiative.

Professor Nielsen is one of the world’s Dr Muhammad Shiddiky of the Trau Group foremost systems biology researchers, and received a NHMCR Career Development Fifty such fellowships are the honour marks the first time the Novo Fellowship for his work in developing a awarded annually to support Nordisk Foundation has awarded a grant to a microfluidic strategy for analysing circulating biotechnology researcher. tumour cells in cancer patients. leading international scientists to conduct research at a CAS The research will seek to understand why Circulating tumour cells detach themselves cancer cells and other rapidly growing from a primary tumour and travel through the institute. mammalian cells produce lactic acid. blood stream, often resulting in secondary Under the fellowship, Professor Whittaker cancer sites. spent one month working in Beijing and at Professor Nielsen’s work will aim to develop the National Centre for Nanoscience and a detailed kinetic and regulatory model of Though they show excellent promise as a Technology. the central carbon metabolism in cultured biomarker, the low abundance of circulating mammalian cells. tumour cells in the blood stream has up until now made their presence difficult to detect. In addition to understanding the differences between healthy and cancerous cells, findings The objective of Dr Shiddiky’s work is the of the research could also be applied to development of a standard diagnostic tool for metabolic diseases such as diabetes. cancer patients undergoing systemic therapy.

42 - AIBN Annual Report 2015 Awards highlight scientific excellence

The top quality science at AIBN was recognised It was the second successive year an AIBN Dr Marcellin’s research program is studying with a number of researchers receiving researcher has won the award, with Professor the production of an industrially organic both local and national awards during 2015, Ernst Wolvetang being the recipient in 2014. insecticide, the development of tools to better highlighting their outstanding contribution to understand microbes, and finding genetic their respective fields. Professor Chengzhong (Michael) Yu was engineering targets. awarded the 2015 Le Fèvre Memorial Prize by Professor Christine Wells won a prestigious the Australian Academy of Science. “It is a great honour to get recognition for the Metcalf prize for her leadership in stem cell work I am doing and I am very grateful for it,” research. Named after the late Professor RJW Le Fèvre Dr Marcellin said. FAA FRS, the prize recognises outstanding Awarded by the National Stem Cell Foundation basic research in chemistry. Fellow Associate Group Leader Dr Zhongfan of Australia, the $50,000 award is named Jia from the Monteiro Group won a UQ after the late Professor Donald Metcalf AC, an Professor Mark Kendall’s ongoing work on Foundation Research Excellence Award for internationally renowned Australian expert on nano projections for vaccine delivery and work to develop a totally plastic battery to haematopoiesis. diagnostics was recognised by professional power future flexible and wearable electronic body Engineers Australia. He was named devices. “It was great recognition for on the organisation’s annual Top 100 Most Influential Engineers list. AIBN Board member “I am very proud and excited to what the lab is doing, and Kathy Hirschfeld was also named on the 2015 the resources we have made list. get this award because it is very competitive,” Dr Jia said. available,” Professor Wells said. AIBN has proudly been consistently represented by its researchers on this annual “This is a big step towards obtaining AIBN’s expertise in stem cell research was list, with former AIBN Director Professor Peter competitive grant funding to progress my further recognised during the year with Gray, former Group Leader Professor Max Lu, career, and it is a big boost for my research,” Professor Justin Cooper-White being awarded and current Group Leader Professor Anton he said. the 2015 Aon Risk Solutions Regenerative Middelberg all being included in past years. Medicine Award by Life Sciences Queensland Dr Jia is the eleventh AIBN recipient of the for his research at the interface of engineering During the annual UQ Research Week UQ Foundation Research Excellence Award, and biology. ceremonies AIBN researchers were recognised including Group Leaders Professor Michael for their contribution to science, and the strong Monteiro, Professor Lianzhou Wang and “I’m proud to receive this award from my potential and novelty of their work. Associate Professor Zhi Ping (Gordon) Xu. peers, and will continue to advocate for the development of regenerative medicine Associate Group Leader Dr Esteban Marcellin to improve clinical outcomes for patients,” from the Nielsen Group won the Science Prize Professor Cooper-White said. of the Partners in Research Excellence Award for his collaboration with The Dow Chemical Company.

AIBN Annual Report 2015 - 43 NEWLY AWARDED RESEARCH FUNDING COMMENCING IN 2015

Granting Funding Total Funding Granting Body Investigators Project Title Scheme Years Awarded Dr Zhi Chen, Prof John Drennan, Dr Development of high performance nanostructured 2015 - 2017 $305,900 Gerard Snyder (Bi, Sb)2Te3 nanomaterials Prof Ajayan Vinu, Prof Mietek Design of functionalized mesoporous fullerenes for 2015 - 2017 $324,700 Jaroniec clean energy Prof Darren Martin, Prof Eric Discovery McFarland, Dr Bronwyn Fox, Dr High performance sustainable carbon fibres from Projects 2015 - 2017 $345,500 Pratheep Annamalai, Dr Bronwyn Australian spinifex grass Laycock Dr Chunxia Zhao, Prof Anton Next generation core-shell materials based on 2015 - 2017 $458,600 Middelberg, Prof Zhengzhong Shao biomolecular dual-templating Prof Kirill Alexandrov Developing orthogonal synthetic signaling cascades 2015 - 2018 $534,700 Future Prof Christine Wells The systems biology of stem cells 2015 - 2018 $931,552 Fellowships Prof Lianzhou Wang, A/Prof Shanqing Zhang, A/Prof Nunzio A new integrated photo-electrochemical device Motta, Prof Eric McFarland, Dr Jung 2015 - 2015 $371,500 fabrication & testing system Ho Yun, Dr Hongxia Wang, Prof Peter Halley, Dr Bin Luo Prof Ben Hankamer, Dr Michael Landsberg Prof Joel Mackay, Prof Reaching new heights in high-resolution electron Linkage 2015 - 2015 $956,000 Robert Parton, Prof Paul Young, Prof microscopy Infrastructure, Michael Monteiro, Dr Daniela Stock Australian Equipment and Research Facilities A/Prof Glenda Gobe, Prof Pam Council Russell, A/Prof John Hooper, Prof Adrian Herington, Prof Christian Langton, A/Prof Kristofer, Prof Ian The Vevo 2100 micro-ultrasound plus LAZR 2015 $55,000 Brereton, Prof Ian Frazer, Dr Andrew photoacoustic imaging platform Fielding, Prof Dietmar Hutmacher, Dr Michelle Hill, Prof Josephine Forbes, Dr Allison Pettit, Dr Kate Johnston Prof Eric McFarland, Prof Darren Martin, Dr Bronwyn Fox, Dr Jorge Cost effective carbon fibres from polyethylene for 2015 - 2017 $159,661 Beltramini, Dr Christopher Derstine, lightweight applications Dr Michael Mills Prof Chengzhong Yu, A/Prof Neena Novel nano-pesticides for animal healthcare 2015 - 2018 $330,000 Mitter, Dr Aleta Knowles A/Prof Kristofer Thurecht, A/Prof Stephen Mahler, Dr Bradley Walsh, Next generation hybrid nanomaterials: bispecific 2015 - 2018 $360,000 Linkage Projects Prof Pamela Russell, Dr Douglas antobodies using antibody-targeted polymers Campbell Prof Han Huang, Prof Lianzhou Understanding the role of nanoparticles in water- Wang, Prof Zheng Jiang, Dr 2015 - 2018 $377,000 based lubrication Jianqiang Zhang Prof Lars Nielsen, Dr Esteban Improving clostridial toxoid production through 2015 - 2018 $488,000 Marcellin Saldana, George Moutafis molecular fermentation maps Prof Kirill Alexandrov, Dr Paul Watt In vitro expression of macrocyclic peptides 2015 - 2018 $670,243 Deciphering the role of atypical DNA methylation Prof Ernst Wolvetang, Dr Ryan Lister, in neural genome regulation and neurological 2015 - 2017 $278,617 Dr Ozren Bogdanovich National Project Grant disorders Health and Prof Ernst Wolvetang, Prof Melissa Directed differentiation of human embryonic stem Medical 2015 $110,000 Little, Prof Justin Cooper-White cells to kidney progenitors Research Career Council Microfluidic strategy for circulating tumour cells Development Dr Muhammad Shiddiky 2015 $52,397 analysis in cancer patients Fellowship Australia- Australian Value adding to agriculture waste by catalytic India Strategic Dr Jorge Beltramini, Dr John Zhu 2015 - 2016 $218,957 Government gasification to bio-fuel production Department of Research Fund Industry and Research Dr Yasmina Sultanbawa, A/Prof Zhi Natural additives to improve quality and shelf life of 2015 - 2016 $143,000 Science Connections Ping Xu animal feeds Australian National Collaborative Research Infrastructure Government Prof Lars Nielsen Strategy: Bioplatforms Australia Project - 2015 - 2016 $250,000 NCRIS Department of Metabolomics Australia-Qld node 2013/2015 Education and National Collaborative Research Infrastructure Prof Justin Cooper-White 2015 - 2016 $1,562,079 Training Strategy: Australian National Fabrication Facility

44 - AIBN Annual Report 2015 Granting Funding Total Funding Granting Body Investigators Project Title Scheme Years Awarded

Accelerate Novel tetravalent dengue virus subunit vaccines Dr David Muller 2015 - 2017 $441,000 Fellowship delivered by Nanopatch Queensland Government Advance Queensland Dr Claudia Vickers Microbial metabolic engineering 2015 $13,585 Women's Academic Fund National Developing of point-of-care biosensors for detection Breast Cancer Innovator Award Prof Kirill Alexandrov, Dr Viktor Stein 2015 - 2016 $220,000 of breast cancer relapse Foundation Asian Office Air Force Defense of Aerospace Research Prof Andrew Whittaker, Dr Hui Peng, Unlocking the structure and dynamics of thin 2015 - 2015 $73,457 Research and Sciences Dr Kevin Jack polymeric films Development Program Prof David Reutens, Prof Matt Cooper, Prof Maree Smith, Prof Andrew Boyd, Prof Linda Richards, Australian Prof Perry Bartlett, Prof Pam Russell, Cancer Major Equipment ACRF Facility for Molecular Imaging Agents in Prof David Walker, A/Prof Rajiv 2015 - 2015 $3,200,000 Research Grant Cancer (AFMIAC) Bhalla, A/Prof Markus Barth, A/Prof Foundation Chiara Palmieri, A/Prof Kristofer Thurecht, Dr Venku Venkatachalam and others Australian Bragg Institute Nuclear Dr Jack Clegg, Dr Alison Edwards, Investigating the oxidation state of Neutron Beam Science and Dr Michael Pfrunder, Dr Samuel bis(diphenylglyoximato) cobalt dihalide catalysts by 2015 - 2015 $1,476 Instrument Technology Richardson, Prof Andrew Whittaker single crystal neutron diffraction Proposal Organisation Dr Simon Puttick, A/Prof Steven Rose, Prof Andrew Boyd, Prof Cure Brain Biomarker Andrew Whittaker, Dr Michael Fay, Developing targeted EphA2 based imaging Cancer Discovery 2015 - 2016 $219,712 A/Prof Stephen Mahler, Dr Brett technology for glioblastoma Foundation Program Grant Stringer, Dr Bryan W. Day, A/Prof Kristofer Thurecht Go8 and Go8 Australia the German - Germany Prof Lianzhou Wang, Dr Hongjun Complex mixed oxide photocatalysts with high Academic Joint Research 2015 - 2016 $20,000 Chen porosity and surface area Exchange Co-operation Service Scheme King Saud Prof Ajayan Vinu, Prof Salem Al- Functional nanoporous materials for renewable Research Grant 2015 $51,329 University Deyab, A/Prof Mohamed El-Newehy energy applications Cook Medical Research & development of polymeric materials for Prof Darren Martin 2015 - 2016 N/A* Australia Pty Ltd medical applications Development of polymer coated surface with a Boeing Defence Prof Michael Monteiro significantly greater surface area to capture and kill 2015 - 2016 N/A* Australia microbes Evaluation of the suitability of cross-linked hydrogels formed from Polyethyleneglycol (PEG), ProteoBioactives Prof Justin Cooper-White Hyaluronic Acid (HA) and Hyaluronic Acid-Pentosan 2015 N/A* Pty Ltd Polysulfate (HA-PPS), alone or in combination with … UniQuest Pty Ltd Prof Darren Martin Pathfinder Funding:condom prototyping project 2015 N/A* Tailorable nanoemulsions as prophylactic Influenza Medicago Dr Frank Sainsbury 2015 N/A* vaccines Prof Chengzhong Yu, A/Prof Neena Development of an advanced Hepatitis B N4 Pharma Ltd 2015 N/A* Contract Mitter nanovaccine for human use - Stage 1 FMC Australasia Research Prof Chengzhong Yu Transfer of Materials 2015 N/A* Pty Ltd Identification of thermostable variants of Phytase Bioproton Pty Ltd Prof Kirill Alexandrov 2015 N/A* for use as an animal feed additive King Saud One-dimensional nanostructured photoanodes for Prof Ajayan Vinu 2015 - 2017 N/A* University efficient solar-driven hydrogen production TenasiTech Pty Prof Darren Martin Research Sub-Contract 2015 N/A* Ltd TenasiTech Pty Prof Darren Martin Nanocomposite R&D 2015 - 2016 N/A* Ltd Dr Bhagirath Chauhan, A/Prof Nufarm Ltd Herbicide nanotechnology efficacy trial 2015 - 2016 N/A* Neena Mitter, A/Prof Zhi Ping Xu Vaxxas Pty Ltd Prof Mark Kendall, Dr David Muller Nanopatch and delivery device for Polio vaccine 2015 N/A* Funding of UQ personnel dedicated to Vaxxas Vaxxas Pty Ltd Prof Mark Kendall 2015 - 2016 N/A* activities Research Mr Shufeng Yang Dr Wenyi Gu, A/Prof Zhi Ping Xu Bio-immunotherapy for cervical cancer 2015 $100,000 Donation

AIBN Annual Report 2015 - 45 Facilities and infrastructure

46 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 47 iNFRASTRUCTURE FACILITATES RESEARCH

Australian National Fabrication Centre for Microscopy and Metabolomics The Queensland Node of Metabolomics Facility – Queensland Microanalysis Australia hosted at AIBN provides scientists The Queensland node of the Australian AIBN is home to one of the four laboratories with access to specialised expertise in the National Fabrication Facility at AIBN is one at UQ that form the Centre for Microscopy and analysis of metabolites (metabolomics), and of eight ANFF nodes in Australia across 21 Microanalysis. a particular focus on bioengineering and institutions. ANFF is an open access network fluxomics. of facilities for both academic researchers and The centre is the Queensland node of the industry. Australian Microscopy and Microanalysis Facility Manager Dr Mark Hodson said the Facility and it facilitates research outcomes facility specialises in understanding cellular Facility Manager Mr Derek Hirons said ANFF-Q through the provision of training, expertise and behaviour at the metabolite and system level. provides specialised facilities and expertise state-of-the-art infrastructure in microscopy “We measure concentrations of endogenous in fabricating microfluidic devices, organic and microanalysis. (and exogenous) metabolites, which are the electronics, biomaterials and semiconductor chemical products of metabolism,” Dr Hodson materials. The CMM houses an impressive array of multi- said. million dollar electron and optical microscopes “Our position at AIBN allows us to service and X-ray analysis instruments. The Centre’s “Studying metabolism extends well beyond the the needs of many of the researchers at the facilities enable much of the imaging and human body – it is the process by which all Institute and The University of Queensland, microanalysis that supports AIBN researchers organisms convert and utilise energy, and thus but also the needs of the Queensland bio and as well as external academic and industry sustain life.” nanotechnology sector,” Mr Hirons said. clients. Understanding this process can result in In 2014, over 100 peer-reviewed publications “The Centre enables researchers to glimpse advances in a diverse range of bioengineering acknowledged the support provided by the world at atomic, nano, molecular, cellular projects, with recent highlights in studies ANFF-Q. and macromolecular scales,” Acting Director Dr involved in the production of biofuels, Kevin Jack said. biopesticides, and bioplastics. “At ANFF-Q, researchers can develop their work from a concept to a prototype, and In 2015 four new state-of-the-art instruments The facility offers its expertise to any project consultation with our Professional Officers can and imaging cameras were commissioned, with a biological question where metabolism clarify potential applications of fabrication and providing improved imaging to existing plays a role. characterisation,” he said. transmission electron microscopes and a range of new capabilities. “Our extensive expertise in metabolomics “All of this can be supported in-house and analytical chemistry extends from pure using our specialised software packages, “The Gatan K2 Summit and OneView cameras service provision to active collaboration fabrication facilities ranging from 10nm to enable a new level of detail to be captured, on study design and grant submission as 70µm resolution, and our suite of imaging and further improving CMM’s already impressive co-investigators that shape the direction of characterisation tools.” capabilities in structural biology and material research projects,” Dr Hodson said. science,” he said. ANFF-Q continued to expand its capabilities In the past two years the Queensland Node in 2015, with the procurement of a JEOL CMM commissioned a new Rigaku X-ray has successfully extended the reach of its IT-300 variable pressure scanning electron Diffractometer, which offers thin-film services, with an increasing number of external microscope allowing for larger sample sizes diffraction and reflectivity, micro-diffraction and contracts and clients. and different compounds such as samples in-situ temperature studies. An FEI focussed- that are wet, oily, non-conductive or outgas ion-beam scanning electron microscope was The Node has worked on a multitude of excessively. also commissioned providing high-resolution projects from very different areas of biology, and 3D imaging, analysis and fabrication for a including studying wheat, maize, sugarcane, The facility’s fabrication capabilities were also range of materials and biological samples. and avocado crops, as well as microbial and extended with the addition of an air objective mammalian cell systems, to either improve for the NanoScribe Photonic Professional GT. The Centre has been particularly involved in yields, disease resistance or increase output of ANFF-Q’s Nanoscribe is the only open-access enabling the imaging of research for AIBN spin- bioengineered products. piece in the world, and is capable of fabricating out companies Vaxxas Pty Ltd and Tenasitech true three-dimensional nanostructures in Pty Ltd, which were formed out of the Kendall “We have also been involved in projects centred numerous photoresists down to 100nm and Martin Groups respectively. The level of on the understanding of disease/treatment, resolution. detail provided by CMM equipment allowed both in animals (equine laminitis) and also both companies to improve their products and humans, such as anaesthesia, diabetes Other equipment secured during the year accelerate their development. (types 1 & 2), chronic kidney disease, bacterial includes an Oxford PlasmaPro NGP80 RIE infection, liver transplantation, and so on.” system for dry etching and plasma deposition, During the year the quality of the provision and an Ultimaker II 3D Printer, which is capable of service CMM provides through its The facility has also been actively involved in of 3D printing at a 20 micron definition, facilities across UQ was recognised with a areas of national importance, investigating allowing for rapid bespoke fabrication at Commendation for the Service award at the UQ organisms from the Great Barrier Reef, with minute scales. Awards for Excellence. interest in signalling and communication in Crown of Thorns starfish as well as marine “Through National Collaborative Research sponge ecology. Further collaborations Infrastructure Strategy funding we have been with researchers are investigating seasonal able to continue to develop our resources and behaviour in crop pest snails, and provision of expertise, and will continue to do so in the expertise to a study on the health and mating future,” Mr Hirons said. behaviour of a Northern Quoll population.

48 - AIBN Annual Report 2015 Cutting edge research is progressing at AIBN in part because of support infrastructure based at the Institute, with expertise in nano-fabrication, metabolomics, biologics, protein expression, stem cells, microscopy and microanalysis

National Biologics Facility Protein Expression Facility StemCore The National Biologics Facility (NBF) assists Providing specialised recombinant protein StemCore is a state-of-the-art Pluripotent Australian researchers with the production of production services across Australia, and Stem Cell Core Facility at AIBN that enables biologics - a relatively new class of therapeutic increasingly drawing international clients, the and accelerates stem cell research for which are produced using a biological process. Protein Expression Facility (PEF) experienced academics and industries working in the area Funded by the National Collaborative Research growth in its output during the year. of regenerative medicine. Infrastructure Strategy, the NBF has two nodes – one at AIBN and the other at CSIRO PEF’s existing relationship with Malaysian The facility offers a range of products and Molecular Health Technologies in Melbourne. biotechnology company Sentinext services, including proprietary human Therapeutics Sdn Bhd was expanded with the embryonic and induced pluripotent stem cells The Queensland node houses a $15 million signing of a contract to deliver a fifth project (iPSCs), cell characterisation, and training. The suite of laboratories, clean rooms and to develop an antivirus vaccine for Enterovirus organisation also produces custom generation equipment, and is staffed by a dedicated 71, which is known for causing hand, foot and of induced pluripotent stem cell lines and team of researchers engaged in antibody mouth disease. differentiation of stem cells into ectodermal, discovery, protein engineering, mammalian mesodermal and endodermal cells. cell line development and biopharmaceutical The facility has also developed a reputation for development. The Queensland node is led by producing virus-like particles for diagnostic During the year, StemCore embarked on an AIBN Group Leaders Professor Peter Gray and and vaccine development in other areas such exciting development phase that will open new Associate Professor Stephen Mahler. as human papillomavirus, hepatitis B, influenza, opportunities to validate stem cell research chikungunya virus, and porcine circovirus. capabilities in Australia. “The National Biologics Facility works together with academia, government, private PEF Director Associate Professor Linda In late 2015 StemCore entered into a strategic research bodies and industry to develop new Lua said the facility’s capabilities make it a partnership with international drug discovery therapeutics and accelerate their path to standout among other service providers. company. commercialisation,” Antibody Development Manager Dr Martina Jones said. “Our strength is in the expertise and skill of General Manager Dr Nilay Thakar said the our staff. Clients come to us with a problem agreement is a milestone for the organisation. Following a number of years manufacturing wanting a solution, and we then design the an experimental antibody (m102.4) to combat experiment, and conduct the experiment all in- “This is StemCore’s first commercial the deadly Hendra virus, 2015 saw the start house with our facilities at the AIBN,” Associate agreement with a large multinational biotech of the Hendra Phase I safety trial. Involving 40 Professor Lua said. company, providing an endorsement of our participants, the trial organised by Q-Pharm will capabilities and paving the way for further report its results in early 2016. In collaboration “Some clients want knowledge-based partnerships with pharmaceutical and with Queensland Health, NBF manufactured results and data, others want materials to be biotechnology companies,” Dr Thakar said. sufficient amounts of m102.4 to complete produced.” the safety trial and stock-pile the antibody for Further developments during the year resulted compassionate use in individuals deemed to In a trend seen among other facilities based in a research agreement with the Translational have had high-risk exposure to Hendra virus. at AIBN, PEF saw growth in the number of Research Institute and the Wolvetang Group external users accessing its services. at AIBN to generate induced pluripotent stem “NBF has showcased its ability to mobilise cells from patients with intellectual disorders. resources in the interest of national safety, “Small biotech companies are increasingly in response to the threat of an emerging seeing us as a viable alternative to heavily “This is an exciting pilot study that brings infectious virus,” Manufacturing Manager Dr investing in their own internal capabilities,” she together the best of Stemcore, AIBN and TRI Jeff Hou said. said. to systematically investigate the mechanisms underlying the development of certain The year also saw the licence of a novel As the Foundation Director of PEF, Associate intellectual disorders and may inform new antibody targeting the dendritic cell marker Professor Lua has seen rapid growth in PEF’s intervention strategies,” he said. CD83 transfer from TransBio Ltd to DendroCyte size and research output since 2004; more than BioTech Pty Ltd for the purpose of developing 220 projects were completed in 2015 for over StemCore serves academic and industry novel therapeutics to prevent and treat graft 40 groups. clients, and is one of the few Australian versus host disease, a common side effect of labs to have successfully employed non- bone marrow transplantation. This antibody Trends point to requests for larger scale protein viral, genome-integration free technology to was isolated at the NBF, followed by the production, resulting in an increased capacity in generate iPSC lines from several diverse and creation of a production level cell line and upstream and downstream processing. challenging diseases such as schizophrenia, bioprocess optimisation. X-linked cerebral palsy, X-linked Chromosomal During the year the facility worked with disorders, Parkinson’s, Down syndrome and The NBF’s reputation is growing within the international non-profit organisation PATH certain intellectual disorders. research sphere, resulting in a marked increase Malaria Diagnostic Development Group, and of external users accessing the facility’s also supported research targeting Middle analytical equipment and services during the East Respiratory Syndrome during the year’s year. outbreak in Asia.

AIBN Annual Report 2015 - 49 Students and graduates

50 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 51 STUDENT DIfference

The AIBN aims to cultivate academically talented individuals with the potential and desire for a successful career in research.

AIBN’s commitment to research excellence is embedded in our quality research programs and the selection of high calibre students for these projects, which positions students to be leaders of exciting science that produces high impact peer-reviewed publications. The dynamic research environment at AIBN also provides opportunities for students to be named on patents for discoveries they are a part of.

Students can engage with AIBN through a range of formal opportunities. These include the Doctor of Philosophy (PhD) and Master of Philosophy (MPhil) programs, undergraduate honours projects, and research internships.

This range of programs allows AIBN to foster individuals throughout the tertiary education lifecycle, and promotes students to develop their abilities and interests at multiple entry points during their studies.

Support AIBN provides a suite of research student support that promotes timely progress through candidature and productive outcomes in the form of publications, patents and industry- ready graduates.

All commencing students receive a $2,000 IT package to ensure efficient and Undergraduate opportunities – Research degrees – contemporary IT infrastructure research internships and honours PhD and MPhil projects from the start. All AIBN PhD and projects MPhil students have a minimum Research internships are available to The Doctor of Philosophy (PhD) and Master undergraduate science and engineering of Philosophy (MPhil) programs are typically living allowance equal to the students from Australian and International 3.5 – 4 years and 1.5-2 years in duration current Australian Postgraduate universities. Research internship projects may respectively. The PhD is the entry qualification be from six weeks to six months, depending on for those who wish to teach and research in an Award (APA) rate. program arrangements. Australian university. Progress through candidature is incentivised by top-up payments of $1,800 for each milestone AIBN hosted 37 undergraduate There are 130 individuals whose that is attained on time. AIBN provides $4,000 PhD or MPhil has been awarded to each research student to present their research interns from research and grow their academic network at universities in Australia, Europe, through AIBN. conferences in Australia and abroad. Asia and the Americas. AIBN’s research higher degree team are always Some interns have returned to AIBN to available to provide advice, administrative and complete further research in their Honours or pastoral support to our RHD students. Masters degrees.

52 - AIBN Annual Report 2015 AIBN Student Association

The research higher degree cohort at AIBN has In conjunction with the Institute for Molecular “This was reflected most in grown to more than 120 students attracted to Bioscience, the ASA organised and promoted the institute from across the globe – spanning a six part statistics workshop, which covered the social and sporting events 26 countries and six continents. This group areas of statistical data analysis of relevance to hosted during the year where, has long been represented by a very active and those working in the fields of molecular biology valued AIBN Student Association (ASA). and biomedical research. in addition to social events the ASA has run in the past, The year saw student representatives from The year saw the return of the AIBN Student nearly every floor of the building lend a hand Conference, which was held in Customs we also collaborated with the on the committee, including ASA President House. With this historic venue as a backdrop, student associations of both Lewis Chambers, Vice-President Hamid students were given the opportunity to Ilbeygi, Secretary Anand Meka, Treasurer showcase the diverse and exciting work that the Institute for Molecular Anna Gemmell, RHD Representative Jessica they undertake at AIBN. The standard of Bioscience and the School Schwaber, Academic Representatives presentations was notably high, and special Manasi Mantri and Abu Sina, and Social congratulations go to Benjamin Ng (Trau of Chemistry and Molecular Representatives Trisha Ghosh, Alireza Group) and Amanda Septevani (Martin Group) Biosciences on a number of Hosseinmardi and Prasanna Abbaraju. who took out the top prizes for best oral and digital poster presentations respectively. events,” Mr Chambers said.

“2015 was a very active year for Delivering the keynote presentation of the The year also saw the first inter-Institute the ASA, hosting 20 sporting, conference, AIBN Industry Fellow Dr Ian Nisbet soccer competition, which featured eight spoke of experiences that have served him best teams from five different institutes, and the social, cultural and academic in the field of biotechnology commercialisation, beginning of an Ultimate Frisbee competition events, with a particular focus and gave his unique insight from a career with the Centre for Advanced Imaging. Within which has spanned both the academic and the Institute, the annual staff versus students’ on integration with the student industrial spheres of scientific research. soccer and cricket matches continue to be associations of other research a source of social interaction and friendly One of the key goals for the ASA in 2015 competition. institutes and schools around was to expand interactions with the student UQ,” ASA President Mr Lewis associations of other institutes. This was to promote cooperation and collaboration not Chambers said. just between students in the different research groups at AIBN, but also within UQ as a whole.

AIBN Annual Report 2015 - 53 RHD Report

The Institute’s research higher degree (RHD) student, signifying the growth the Institute has Graduate School, enabling students to travel student cohort has maintained a high level experienced since its inception. The first annual to conferences and visit external institutions to of enrolment. An additional 34 Doctor of report published by AIBN for 2004 shows six further their education. Philosophy (PhD) and five Master of Philosophy students enrolled. Fast forwarding to the 2008 (MPhil) students commenced projects in 2015. annual report, AIBN celebrated the completion AIBN’s outreach program to provide The RHD cohort of 122 candidates included 30 of its first PhD student, Dr Akshat Tanksale, opportunities to high-achieving undergraduate Australian and 92 international students. in December of that year, and the Institute’s students continued through Summer and student cohort then numbered 64. Winter research internship programs, which collectively hosted 37 high-calibre students In 2015 another 29 PhDs and It was therefore of particular significance and from UQ, as well as other Australian and two MPhils were awarded pride that Dr Nicholas Fletcher graduated in international universities. bringing the total number of March 2015, as part of a cohort nearly double the size of that in 2008. Students are at the These four to 12 week RHD graduates to 130. The heart of AIBN – a commitment shown by research student cohort has research groups and supervisors to support opportunities embed students and provide the opportunity to train budding within research groups, been sustained above 120 for researchers, and in turn a commitment by students to further the pursuit of scientific engaging them in authentic several years and features a research undertaken at the Institute. regular intake and graduation research projects at the host of approximately 30 students The AIBN research experience is enriched by laboratories. More than 200 the great diversity of our people, the quality of students have now successfully annually. Impressively, we are their research and its potential translation to products and technologies that will improve participated in the Summer and seeing a consistent reduction human life and our environment. in the time taken to complete Winter research internships a PhD at AIBN to well below 4 AIBN’s research students are men and since the program’s inception in women from 26 nations who are pursuing 2007. years, largely supported by the new knowledge in fields across the science and engineering disciplines. The quality of The AIBN internship program has an alumni well-defined milestone structure our students is evident in their success in that includes many students who have obtaining competitively awarded scholarships. and financial and pastoral acquired a passion for research, transitioning In 2015 more than 34 merit-based scholarships through honours research projects to PhD support provided to all students. were awarded to commencing RHD students and into post-doctoral research. Current PhD at AIBN. A further 11 travel scholarships A significant milestone for the Institute in 2015 students Rebecca Lane, Lewis Chambers and were awarded to AIBN students by the UQ was the graduation of our one hundredth PhD

54 - AIBN Annual Report 2015 The Institute’s research higher degree (RHD) student cohort has maintained a high level of enrolment. An additional 34 Doctor of Philosophy (PhD) and five Master of Philosophy (MPhil) students commenced projects in 2015. The RHD cohort of 122 candidates included 30 Australian and 92 international students.

Ellen Otte, as well as AIBN graduates Dr Amanda Pearce, Dr Nathan Boase and Dr Nick Fletcher are all among the students who were introduced to AIBN as undergraduates and participated in the internship program.

2015 also marked the second year of hosting students from Fudan University on a student exchange program over six weeks. The visits are part of a wider UQ initiative to engage with Chinese institutions, and build on AIBN’s already strong ties with Fudan University through the work of Professor Chengzhong (Michael) Yu and Professor Andrew Whittaker.

AIBN also hosts Industry placements from UQ’s growing Bachelor of Engineering/ Master of Engineering (BE/ME) program. These placements provide hands-on experience to emerging, talented students who aspire to a career in research.

The Institute is proud of its track record of attracting research talent from geographically and academically diverse sources. In addition to the 92 international students from 25 countries enrolled in 2015, the research intern program attracted a further four overseas students. AIBN has the second highest rate of international RHD enrolment within the university, and this ability to draw overseas students highlights its reputation on an international level.

A further focus is to attract emerging talent from other universities in south-east Queensland and Australia more broadly. The RHD team attends key student career fairs and expos throughout the year to promote the Institute and recruit local students who exhibit academic excellence.

After taking on the duty of AIBN Deputy Director (Graduate Studies) in 2012, Professor Darren Martin stepped down at the end of the year, with the appointment passing to Professor Chengzhong (Michael) Yu. The Institute is grateful of Professor Martin’s service in the role.

AIBN Annual Report 2015 - 55 research higher degree students

Students form a significant proportion of AIBN’s research personnel, and we recognise the following students who were enrolled at the Institute during 2015 and contributed to AIBN’s successes during the year.

Prasanna Lakshmi Abbaraju Donna Capararo Ricardo Axayacatl Gonzalez Garcia Rufika Shari Abidin Ian Cartmill Stephen Goodall Yusilawati Ahmad Nor Lewis Charles Chambers Yadveer Grewal Mostak Ahmed Ao Chen Zhengying (Allison) Gu Dewan Taslima Akhter Liyu Chen Gency Gunasingh Md. Mahbub Alam Weiyu Chen Michael James Hayter Suad Alateeq Xiaoli Chen Matthew Henry Mohamed Ahmed M Alfaleh Panagiotis Chrysanthopoulos Hadi Hezaveh Samah Alharbi Jacob Coffey Alejandro Hidalgo-Gonzalez Eid Alosime Noor Dashti Elizabeth Hines Abdul Karim Al Sultan Renato de Souza Pinto Lemgruber Alireza Hosseinmardi Will Anderson Shuvashis Dey Cheng Huang Aditya Ardana Thanh Tam Doan Yue Hui Waqas Aslam Amir Hossein Farokh Niaei Alessandro Iovino Luqman Atanda Julia Fischer Jiaul Islam Thejus Baby Nicholas Fletcher Manasi Jambhrunkar Anushree Balachandran Patrick Fortuna Edward Jiang Thomas Bennett Jianye Fu Zhen Jiang Nathan Boase Wanli Johnny Fu Andrew Johnston Valentin Bobrin Mikhail Gavrilov Mohammad Kalantari Mareike Bongers Anna Gemmell Li Pin Kao Marion Brunck Trisha Ghosh Kamil Reza Khondakar Teera Butburee Marsel Gokovi Maisheng Koo Suzanne Kathryn Butcher Arghavan Golbaz Hagh Rebecca Emily Lane

56 - AIBN Annual Report 2015 Geoffrey Lawrence Ellen Otte Kanupriya Tiwari Kebaneilwe Lebani Harish Padmanabhan Nguyen Tran Hong Seng Lee Amanda Pearce Nicole van der Burg Chang Lei Bingyin Peng Dhanunjaya Naidu Vangapandu U Wang Lei Nicolas Pichon Jarurin Waneesor Bei Beatrice Li Clementine Pradal Haofei (Faye) Wang Juan Li Ramanathan Pudhukode Vaidyanathan Jing Wang Lim, Soo Swasmi Purwajanti Yue Wang Sharon Chien-Yu Lin Lyndon Raftery Jonathan Wei Yang Liu Rui Ran Yangyang Wen Yun Liu Masih Rashidi David Wibowo Derong Lu Gishan Ratnayake Thomas Williams Jennifer Lu Irene Reto Li-Yen Wong Carlos Luna Flores Samuel Richardson Yanheng Wu Yiming Ma Kye Jakob Robinson Yilun Wu Vijayan Manoharan Tim Ruder Mu Xiao Elizabeth Mason Pedro Andres Eduardo Saa Higuera Chun Xu Aini Syahida Mat Yassim Andrea Schaller Shiyu Yan Sainimili Vaubula Mateyawa Jennifer Schoning Guang Ze Yang Nicholas Matigian Jessica Schwaber Tianyu Yang Alireza Mayahi Athanasia Amanda Septevani Yannan Yang Timothy McCubbin Arjun Seth Nicolas Eugenio Zaragoza Michael Mehlman Abu Ali Ibn Sina Bijun Zeng Anand Kumar Meka Mitchell John Smith Cheng Zhang Khairatun Najwa Mohd Amin Mohammad Soheilmoghaddam Hongwei Zhang Shehzahdi Moonshi Faheem Amir Solangi Jun Zhang Fatemeh Movahedi Hao Song Kai Zhang Swathi Mukundan Michael Song Min Zhang Taryn Naidoo Marcos Saul Soto Perez Liang Zhao Amir Nemati Hayati Qi David Sun Yongmei Zhao Benjamin Yong Chou Ng Xiaoran Sun Ruifeng Zhou Hwee Ing Ng Jie Tang Xiaobo Zhu Yuting Niu Hossam Tayeb Yingdong Zhu Owen Noonan Karin Taylor Huali Zuo Camila Orellana Alemu Tekewe Mogus

* The list includes graduating students and those in a UQ RHD program undertaken at AIBN during 2015, and excludes withdrawn students.

AIBN Annual Report 2015 - 57 2015 gRADUATE LIST

Samah Alharbi specificity for tumours in vivo. Chemical McQualter, et al., (2014) The use of an Qualifications: Bachelor of Medical Sciences Communications, 49 37: 3836-3838. doi:10.1039/ acetoacetyl-CoA synthase in place of a (Pathology Medicine) - Umm Al Qura c3cc00127j. ?-ketothiolase enhances poly-3-hydroxybutyrate University, Master of Clinical Sciences (Clinical Boase, et al., (2012) Molecular imaging with production in sugarcane mesophyll cells. Physiology) with Honors - Griffith University polymers. Polymer Chemistry, 3 6: 1384-1389. Plant Biotechnology Journal, 13 5: 700-707. PhD Awarded: July doi:10.1039/c2py20132a. doi:10.1111/pbi.12298. Principal Supervisor: Professor Ernst Conwell, et al., (2014). 1,5-Anhydroglucitol, Wolvetang Marion Brunck an indicator of short term glycaemic control, is Thesis Title: Generation of Intestinal Stem Qualifications: BSc with Honours (Class the most discriminatory metabolomic marker Cells from Human Pluripotent Stem Cells and I) Immunology stream - University of in adolescents with type 1 diabetes compared Partially Reprogrammed Mouse Fibroblast Queensland, BSc in Biological Sciences to control subjects. In: APPES & APEG 2014: Using a Novel LGR5 Promoter-Based Reporter. (Immunology) - University of Queensland Asia Pacific Paediatric Endocrine Society & Publications: PhD Awarded: June Australasian Paediatric Endocrine Group Joint Ovchinnikov, et al., (2014) Transgenic human Principal Supervisor: Professor Lars Nielsen Annual Scientific Meeting, Darwin, NT, Australia, ES and iPS reporter cell lines for identification Thesis Title: RNA CaptureSeq: Targeted 29 October-1 November, 2014. and selection of pluripotent stem cells in vitro. Sequencing for Comprehensive Transcriptome Behrendorff, et al., (2013) 2,2-Diphenyl- Stem Cell Research, 13 2: 251-261. doi:10.1016/j. Studies. 1-picrylhydrazyl as a screening tool for scr.2014.05.006. Publications: recombinant monoterpene biosynthesis. Schwaber, et al., (2015) Filling the void: Microbial Cell Factories, 12 76: 1-11. Abdul Karim Al Sultan allogeneic myeloid cells for transplantation. doi:10.1186/1475-2859-12-76. Qualifications: Bachelor of Pharmacy Current Opinion in Hematology, 1-6. doi:10.1097/ Dietmair, et al., (2012) Metabolite profiling of (Pharmaceutical and Industrial Pharmacy) - MOH.0000000000000205. CHO cells with different growth characteristics. King Saud University, Master of Biotechnology Clark, et al., (2015) Quantitative gene profiling Biotechnology and Bioengineering, 109 6: 1404- (BioPharmaceutical and Bioprocessing of long noncoding RNAs with targeted RNA 1414. doi:10.1002/bit.24496. Industry) - University of Queensland sequencing. Nature Methods, 12 4: 339-342. Dietmair, et al., (2012). Metabolomic PhD Awarded: June doi:10.1038/nmeth.3321. analysis of CHO cultures with different growth Principal Supervisor: Associate Professor Mercer, et al., (2015) Genome-wide characteristics – development of a metabolite Stephen Mahler discovery of human splicing branchpoints. extraction protocol for suspension adapted mammalian cells. In Nigel Jenkins, Niall Barron Thesis Title: Beyond Antibodies: Development Genome Research, 25 2: 290-303. doi:10.1101/ and Paula Alves (Ed.), Proceedings of the of a Novel Molecular Scaffold Based on Human gr.182899.114. 21st Annual Meeting of the European Society Chaperonin 10. Brunck, et al., (2014) Absolute counting of neutrophils in whole blood using flow for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 (pp. 37-41) Dordrecht, Nathan Boase cytometry. Cytometry Part A, 85 12: 1057-1064. doi:10.1002/cyto.a.22503. Netherlands: Springer. Qualifications: Bachelor of Science (Honours) -  Brunck, et al., (2014) Concise review: next- Constantinou, et al., (2011) GC-MS University of Queensland metabolomic analysis reveals significant PhD Awarded: July generation cell therapies to prevent infections in neutropenic patients. Stem Cells Translational alterations in cerebellar metabolic physiology in Principal Supervisor: Associate Professor a mouse model of adult onset hypothyroidism. Kristofer Thurecht Medicine, 3 4: 541-548. doi:10.5966/sctm.2013- 0145. Journal of Proteome Research, 10 2: 869-879. Thesis Title: Hyperbranched Polymers for In doi:10.1021/pr100699m. Vivo Multimodal Molecular Imaging. Xiaoli Chen Publications: Jacob Coffey Ma, et al., (2015) The in vivo fate of Qualifications: Bachelor of Engineering (Bioengineering) - Nanyang Technological Qualifications: Bachelor of Science nanoparticles and nanoparticle-loaded (Nanotechnology) - University of New South microcapsules after oral administration in mice: University Wales, Diploma of Innovation Management - evaluation of their potential for colon-specific PhD Awarded: December University of New South Wales delivery. European Journal of Pharmaceutics Principal Supervisor: Professor Peter Gray PhD Awarded: October and Biopharmaceutics, 94393-403. Thesis Title: Development of a Culture Principal Supervisor: Professor Mark Kendall doi:10.1016/j.ejpb.2015.06.014. Platform for the Expansion of Pluripotent Thesis Title: Microprojection arrays for Eliezar, et al., (2015) In vivo evaluation of Human Embryonic Stem Cells Cells with the selective capture of circulating biomarkers in folate decorated cross-linked micelles for use of Nanopolymers vivo from the skin the delivery of platinum anticancer drugs. Publications: Publications: Biomacromolecules, 16 2: 515-523. doi:10.1021/ Chen, et al., (2014) Thermoresponsive worms Robinson, et al., (2015) Comparison between bm501558d. for expansion and release of human embryonic polyethylene glycol and zwitterionic polymers Puttick, et al., (2014) Imaging tumour stem cells. Biomacromolecules, 15 3: 844-855. as antifouling coatings on wearable devices for distribution of a polymeric drug delivery doi:10.1021/bm401702h. selective antigen capture from biological tissue. platform in vivo by PET-MRI. Journal of Chemical Biointerphases, 10 4: 04A305.1-04A305.11. Technology and Biotechnology. doi:10.1002/ Panagiotis Chrysanthopoulos doi:10.1116/1.4932055. jctb.4489. Qualifications: Bachelor of Science - University Corrie, et al., (2015) Blood, sweat, and tears: Boase, et al., (2014) Synthesis of a of Patras developing clinically relevant protein biosensors multimodal molecular imaging probe based on PhD Awarded: May for integrated body fluid analysis. Analyst, 140 a hyperbranched polymer architecture. Polymer Principal Supervisor: Professor Lars Nielsen 13: 4350-4364. doi:10.1039/c5an00464k. Chemistry, 5 15: 4450-4458. doi:10.1039/ Thesis Title: Systems Level Signalling Analysis Lee, et al., (2014) Capture of the circulating c4py00513a. A Case Study of Growth Hormone Receptor Plasmodium falciparum biomarker HRP2 in a Rolfe, et al., (2014) Multimodal polymer Signalling. multiplexed format, via a wearable skin patch. nanoparticles with combined 19F magnetic Publications: Analytical Chemistry, 86 20: 10474-10483. resonance and optical detection for tunable, Bongers, et al., (2015) Systems analysis of doi:10.1021/ac5031682. targeted, multimodal imaging in vivo. Journal of methylerythritol-phosphate pathway flux in E. Depelsenaire, et al., (2014) Colocalization the American Chemical Society, 136 6: 2413-2419. coli: Insights into the role of oxidative stress of cell death with antigen deposition in skin doi:10.1021/ja410351h. and the validity of lycopene as an isoprenoid enhances vaccine immunogenicity. The Journal Coles, et al., (2013) Aptamer-targeted reporter metabolite. Microbial Cell Factories, 14 . of Investigative Dermatology, 134 9: 2361-2370. hyperbranched polymers: towards greater doi:10.1186/s12934-015-0381-7. doi:10.1038/jid.2014.174.

58 - AIBN Annual Report 2015 Meliga,et al., (2014). A validated Technology and Biotechnology, 90 7: 1230-1236. 55 417-422. doi:10.1016/j.bios.2013.12.043. computational approach to study skin doi:10.1002/jctb.4545. Grewal, et al., (2013) Label-free mechanical interaction with dynamically-applied Goodall, et al., (2014) An EGFR targeting electrochemical detection of an Entamoeba microneedle and microprojection arrays. In: nanoparticle self assembled from a histolytica antigen using cell-free yeast-scFv NanoBio Australia 2014, Brisbane, Australia, 6 - thermoresponsive polymer. Journal of Chemical probes. Chemical Communications, 49 15: 1551- 10 July 2014. Technology and Biotechnology, 90 7: 1222-1229. 1553. doi:10.1039/c2cc38882k. Yeow, et al., (2013) Surface modification and doi:10.1002/jctb.4509. Patents: Affinity Reagents characterization of polycarbonate microdevices for capture of circulating biomarkers, both in Yadveer Grewal Alejandro Hidalgo-Gonzalez vitro and in vivo. Analytical Chemistry, 85 21: Qualifications: Bachelor of Information Qualifications: Bachelor of Biotechnology 10196-10204. doi:10.1021/ac402942x Technology (Software Architecture)/Bachelor Engineering - Costa Rica Institute of Meliga, et al., (2013). Rational design of of Applied Science (Biochemistry) - Queensland Technology microprojection array-mediated vaccine University of Technology, Bachelor of Applied PhD Awarded: May delivery to skin, using mathematical modelling Science First Class Honours (Life Science) - Principal Supervisor: Professor Justin Cooper- and experimental methods. In: 13AH: Vaccine Queensland University of Technology White Delivery and Stabilization: Improving the Reach PhD Awarded: November Thesis Title: In Vitro Ischemia-Reperfusion of Vaccines, Boston, MA, USA, 8-10 September, Principal Supervisor: Professor Matt Trau Injury Model for Pharmacological Post 2013. Thesis Title: Nanoyeast Antibody Fragments: Conditioning of Human Pluripotent Stem Cell-  Meliga, et al., (2013). Improving vaccine Engineering the Next Generation of Molecular Derived Cardiomyocytes targeting to skin using mathematical model- Diagnostics Publications: driven design and application of microprojection Publications: Titmarsh, et al., (2015). Multiplexed arrays. In: Skin Vaccination Summit, Seattle, Vaidyanathan, et al., (2015) Enhancing protein Microbioreactor Arrays for Rapid Optimisation Washington, USA, 4 - 6 September 2013. capture using a combination of nanoyeast of Pluripotent Stem Cell Maintenance and  Corrie, et al., (2013). In Vivo Biomarker single-chain fragment affinity reagents and Differentiation. In: Tissue Engineering Part Capture Via the Skin Using Surface-Modified alternating current electrohydrodynamic forces. a. 4th TERMIS World Congress, Boston Ma, Microprojection Arrays. In: Proceedings of the Analytical Chemistry, 87 23: 11673-11681. (S335-S336). Sep 08-11, 2015. Asme 2nd Global Congress On Nanoengineering doi:10.1021/acs.analchem.5b02490 Ovchinnikov, et al., (2014) Isolation of for Medicine and Biology. 2nd ASME Global Grewal, et al., (2015) Structural contractile cardiomyocytes from human Congress on NanoEngineering for Medicine and characterization of nanoyeast single-chain pluripotent stem-cell-derived cardiomyogenic Biology, Boston MA., United States, (9-10). 04-06 fragment variable affinity reagents. The Journal cultures using a human NCX1-EGFP reporter. February 2013. doi:10.1115/NEMB2013-93215. of Physical Chemistry Part C: Nanomaterials, Stem Cells and Development, 24 1: 11-20.  Coffey, et al., (2013) Early circulating Interfaces and Hard Matter, 119 22: 12674- doi:10.1089/scd.2014.0195. biomarker detection using a wearable 12680. doi:10.1021/acs.jpcc.5b01234. Ovchinnikov, et al., (2014) Transgenic human microprojection array skin patch. Wang, et al., (2014) Duplex microfluidic SERS ES and iPS reporter cell lines for identification Biomaterials, 34 37: 9572-9583. doi:10.1016/j. detection of pathogen antigens with nanoyeast and selection of pluripotent stem cells in vitro. biomaterials.2013.08.078. single-chain variable fragments. Analytical Stem Cell Research, 13 2: 251-261. doi:10.1016/j. Muller, et al., (2012) Surface modified Chemistry, 86 19: 9930-9938. doi:10.1021/ scr.2014.05.006. microprojection arrays for the selective ac5027012. extraction of the dengue virus NS1 protein as Grewal, et al., (2014) Nano-yeast–scFv a marker for disease. Analytical Chemistry, 84 7: probes on screen-printed gold electrodes for 3262-3268. doi:10.1021/ac2034387. detection of Entamoeba histolytica antigens in a biological matrix. Biosensors and Bioelectronics, Nicholas Fletcher Qualifications: Bachelor of Biotechnology First Class Honours (Nanotechnology) University of Queensland PhD Awarded: March Principal Supervisor: Professor Andrew Whittaker Thesis Title: Bioproduction and self-assembly of designer peptides Publications: Fuchs,et al., (2015) Evaluation of Polymeric Nanomedicines Targeted to PSMA: effect of Ligand on Targeting Efficiency. Biomacromolecules. doi:10.1021/acs. biomac.5b00913.

Stephen Goodall Qualifications: Bachelor of Applied Science (Applied Chemistry) - Queensland Institute of Technology, Master of Applied Science - Queensland University of Technology, Master of Business Administration - Queensland University of Technology PhD Awarded: October Principal Supervisor: Associate Professor Stephen Mahler Thesis Title: Design, Assembly and Characterisation of a Multifunctional, Self- Assembled Nanoparticle incorporating Antibody Fragments Publications: Goodall, et al., (2014) Monoclonal antibody- targeted polymeric nanoparticles for cancer therapy - future prospects. Journal of Chemical Technology and Biotechnology, 90 7: 1169-1176. doi:10.1002/jctb.4555. Buecheler, et al., (2014) Development of a protein nanoparticle platform for targeting EGFR expressing cancer cells. Journal of Chemical

AIBN Annual Report 2015 - 59 Li Pin Kao phosphoproteome. Molecular and Cellular Michael Mehlman Qualifications: Bachelor of Science Proteomics, 13 5: 1219-1230. doi:10.1074/mcp. Qualifications: (Biochemistry) - University of Otago, Master of M113.033951. MPhil Awarded: June Science (Biochemical Engineering) - National Principal Supervisor: Professor Justin Cooper- Chiao Tung University Sharon Chien-Yu Lin White PhD Awarded: March Qualifications: Bachelor of Biotechnology Thesis Title: Effects of hyaluronan-pentosan Principal Supervisor: Professor Ernst Wolvetang (Honours Class IIA) - University of Queensland polysulfate and bone morphogenetic protein-6 Thesis Title: Regulation of Mitochondrial PhD Awarded: July on human mesenchymal stem cells Biogenesis in Human Embryonic Stem Cells Principal Supervisor: Professor Traian Chirila Publications: Thesis Title: Production and in vitro Evaluation Yuting Niu Turner, et al., (2014) Metabolic profiling and of Macroporous Alginate Hydrogel Fibres for Qualifications: Bachelor of Medicine - Wuhan flux analysis of MEL-2 human embryonic stem Nerve Tissue Engineering University, Master of Medicine (Prosthodontics) cells during exponential growth at physiological - Wuhan University and atmospheric oxygen concentrations. PLoS Yiming Ma PhD Awarded: December One, 9 11: e112757.1-e112757.13. doi:10.1371/ Qualifications: Principal Supervisor: Professor Chengzhong journal.pone.0112757. PhD Awarded: May Yu Briggs, et al., (2013) Integration-free induced Principal Supervisor: Associate Professor Thesis Title: Bio-inspired Virus-mimicking pluripotent stem cells model genetic and neural Kristofer Thurecht Nanoparticles for Efficient Cellular Delivery developmental features of Down Syndrome Thesis Title: Controlled delivery of Publications: etiology. Stem Cells, 31 3: 467-478. doi:10.1002/ nanoparticles to the colon for tumour targeting Niu, et al., (2015) Synthesis of silica stem.1297. Publications: nanoparticles with controllable surface Ma, et al., (2015) The in vivo fate of roughness for therapeutic protein delivery. Kebaneilwe Lebani nanoparticles and nanoparticle-loaded Journal of Materials Chemistry B, 3 43: 8477- Qualifications: Bachelor of Biomedical Science microcapsules after oral administration in mice: 8485. doi:10.1039/c5tb01405k. - Monash University, Master of Biotechnology - evaluation of their potential for colon-specific Ahmad, et al., (2015) Shaping nanoparticles University of Queensland delivery. European Journal of Pharmaceutics and with hydrophilic compositions and hydrophobic PhD Awarded: March Biopharmaceutics, 94 393-403. doi:10.1016/j. properties as nanocarriers for antibiotic delivery. Principal Supervisor: Associate Professor ejpb.2015.06.014. ACS Central Science, 1 328-334. doi:10.1021/ Stephen Mahler acscentsci.5b00199. Thesis Title: Antibody Discovery for Sainimili Vaubula Mateyawa Yang, et al., (2015) Biphasic synthesis of Development of a Serotyping Dengue Virus Qualifications: Bachelor of Science - University large-pore and well-dispersed benzene bridged NS1 Capture Assay of the South Pacific , Postgraduate Diploma mesoporous organosilica nanoparticles for of Chemistry - University of the South Pacific, intracellular protein delivery. Small, 11 23: 2743- Soo Lim Master of Science - University of the South 2749. doi:10.1002/smll.201402779. Qualifications: Bachelor of Information Pacific Jambhrunkar, et al., (2014) Effect of Technology (Computing) - Monash University, PhD Awarded: June surface functionality of silica nanoparticles Bachellor of Plant Science - Seoul National Principal Supervisor: Professor Peter Halley on cellular uptake and cytotoxicity. Molecular University, Master of Applied Science Thesis Title: Understanding the role of water- Pharmaceutics, 11 10: 3642-3655. doi:10.1021/ (Bioinformatics) - University of Sydney ionic liquid mixtures on the gelatinization and mp500385n. PhD Awarded: April dissolution of maize starches Yu, et al., (2014) An approach to prepare Principal Supervisor: Professor Lars Nielsen Publications: polyethylenimine functionalized silica-based Thesis Title: Quantitative bacterial Mateyawa, et al., (2013) Effect of the ionic spheres with small size for siRNA delivery. ACS phosphoproteomics liquid 1-ethyl-3-methylimidazolium acetate on Applied Materials and Interfaces, 6 18: 15626- Publications: the phase transition of starch: dissolution or 15631. doi:10.1021/am503060n. Xu, et al., (2014) Rod-like mesoporous silica Lim, et al., (2015) Global dynamics of gelatinization? Carbohydrate Polymers, 94 1: 520- Escherichia coli phosphoproteome in central 530. doi:10.1016/j.carbpol.2013.01.024. nanoparticles with rough surfaces for enhanced carbon metabolism under changing culture Xie, et al., (2013). Starch-based bioplastics: cellular delivery. Journal of Materials Chemistry B, conditions. Journal of Proteomics, 126 24-33. using ionic liquid as a novel plasticiser for 2 3: 253-256. doi:10.1039/c3tb21431a.  doi:10.1016/j.jprot.2015.05.021. improving the processibility of starch polymers. Niu, et al., (2013) Nanoparticles mimicking viral surface topography for enhanced cellular Arifin, et al., (2014) Escherichia coli W shows In: EP’2013: The Sixth International Symposium fast, highly oxidative sucrose metabolism and on Engineering Plastics, Xiamen, China, (121- delivery. Advanced Materials, 25 43: 6233-6237. low acetate formation. Applied Microbiology and 122). 25-28 August, 2013. doi:10.1002/adma.201302737.  Biotechnology, 98 21: 9033-9044. doi:10.1007/ Xie, et al., (2013). Ionic liquid plasticised Niu, et al., (2012) Recent advance in silica- s00253-014-5956-4. starch-based materials. In: 4th International based engineered nanoparticles for gene therapy. Therapeutic Delivery, 3 10: 1217-1237. Licona-Cassani, et al., (2014) Temporal Conference on Biodegradable and Biobased dynamics of the Saccharopolyspora erythraea Polymers (BIOPOL 2013), Rome, Italy, (). 1-3 doi:10.4155/tde.12.98. October 2013.

60 - AIBN Annual Report 2015 Clementine Pradal Vaidyanathan, et al., (2014) Alternating pathways for nuclear delivery of plasmid DNA Qualifications: Bachelor of General Engineering current electrohydrodynamics induced complexed with highly efficient endosome - Ecole Centrale Paris, Master of General nanoshearing and fluid micromixing for specific escape polymers. Biomacromolecules, 15 10: Engineering - Ecole Centrale Paris, Master of capture of cancer cells. Chemistry: A European 3569-3576. doi:10.1021/bm5008376. Biotechnology - University of Queensland Journal, 20 13: 3724-3729. doi:10.1002/ Hou, et al., (2012). Analysis of protein PhD Awarded: March chem.201304590. expression via alternate 3’ Untranslated Region Principal Supervisor: Professor Justin Cooper- Vaidyanathan, et al., (2014) Tuneable “Nano- (UTR) signals through the use of site specific White Shearing”: a physical mechanism to displace recombination. In Nigel Jenkins, Niall Barron Thesis Title: Cellular Delivery nonspecific cell adhesion during rare cell and Paula Alves (Ed.), Proceedings of the Publications: detection. Analytical Chemistry, 86 4: 2042-2049. 21st Annual Meeting of the European Society Pradal, et al., (2015) Hydrolytically degradable doi:10.1021/ac4032516. for Animal Cell Technology (ESACT), Dublin, polyrotaxane hydrogels for drug and cell delivery Shiddiky, et al., (2014) Molecular Ireland, June 7-10, 2009 (pp. 47-51) Dordrecht, applications. Biomacromolecules, 16 1: 389-403. nanoshearing: an innovative approach to shear Netherlands: Springer. doi:10.1021/bm501615p. off molecules with AC-induced nanoscopic Codamo, et al., (2012). An optimised Pradal, et al., (2013) Gelation Kinetics fluid flow. Scientific Reports, 4 3716.1-3716.7. transfection platform for the Epi-CHO transient and Viscoelastic Properties of Pluronic and doi:10.1038/srep03716. expression system in serum-free media. In Nigel alpha-Cyclodextrin-Based Pseudopolyrotaxane Vaidyanathan, et al., (2014). A Multiplex Jenkins, Niall Barron and Paula Alves (Ed.), Hydrogels. Biomacromolecules, 14 10: 3780- Device Based on Tunable Nanoshear Forces Proceedings of the 21st Annual Meeting of the 3792. doi:10.1021/bm401168h. for Highly Specific Detection of Multiple European Society for Animal Cell Technology Protein Biomarkers. In: Proceedings of the (ESACT), Dublin, Ireland, June 7-10, 2009 Ramanathan Pudhukode Vaidyanathan 18th International Conference on Miniaturized (pp. 19-23) Dordrecht, Netherlands: Springer. Qualifications: Bachelor of Technology Systems for Chemistry and Life Sciences doi:10.1007/978-94-007-0884-6_3. (Biotechnology) - Arulmigu Kalasalingam (MicroTAS). 18th International Conference on Song, et al., (2011) Clonal selection of high College of Engineering, Master of Research Miniaturized Systems for Chemistry and Life producing, stably transfected HEK293 cell Biomedical Science (Cell Engineering) - Sciences, San Antonio, TX United States, (1972- lines utilizing modified, high-throughput FACS University of Glasgow 1974). 26-30 October 2014. screening. Journal of Chemical Technology and PhD Awarded: October Shiddiky, et al., (2014). Detecting Exosomes Biotechnology, 86 7: 935-941. doi:10.1002/ Principal Supervisor: Dr Muhammad Johirul Specifically: a Microfluidic Approach Based jctb.2618. Alam Shiddiky on Alternating Current Electrohydrodynamic Codamo, et al., (2011) Enhanced CHO Thesis Title: Nanoshearing: A Microfluidic Induced Nanoshearing. In: Proceedings of the cell-based transient gene expression with Approach for Isolation and Enumeration of 18th International Conference on Miniaturized the Epi-CHO expression system. Molecular Cancer Biomarkers Systems for Chemistry and Life Sciences Biotechnology, 48 2: 109-115. doi:10.1007/ Publications: (MicroTAS). 18th International Conference on s12033-010-9351-9. Miniaturized Systems for Chemistry and Life Vaidyanathan, et al., (2015) Enhancing protein capture using a combination of nanoyeast Sciences, San Antonio, TX United States, (674- Karin Taylor single-chain fragment affinity reagents and 676). 26-30 October 2014. Qualifications: Bachelor of Science (Genetics)  alternating current electrohydrodynamic forces. Curtis, et al., (2013) Cell interactions at - University of Pretoria, Bachelor of Science Analytical Chemistry, 87 23: 11673-11681. the nanoscale: piezoelectric stimulation. IEEE (Honours Class IIA) - University of Queensland doi:10.1021/acs.analchem.5b02490 Transactions On Nanobioscience, 12 3: 247-254. PhD Awarded: August doi:10.1109/TNB.2013.2257837. Wang, et al., (2015) Enabling rapid and Principal Supervisor: Associate Professor  specific surface enhanced raman scattering Shiddiky, et al., (2013). Tuneable Stephen Mahler immunoassay using nano-scaled surface shear “nanoshearing”: an innovative mechanism for Thesis Title: Engineering bispecific antibodies forces. ACS Nano, 9 6: 6354-6362. doi:10.1021/ the accurate and specific capture of rare cancer for targeted delivery of cytotoxin-loaded acsnano.5b01929. cells. In: Roland Zengerle, The 17th International nanoparticles to tumour cells Conference on Miniaturised Systems for Vaidyanathan, et al., (2015) A multiplexed Publications: device based on tunable nanoshearing for Chemistry and Life Sciences: TAS 2013. Taylor, et al., (2014) Nanocell targeting using specific detection of multiple protein biomarkers Proceedings. TAS 2013: The 17th International engineered bispecific antibodies. mAbs, 7 1: 53- in serum. Scientific Reports, 5 9756.1-9756.7. Conference on Miniaturised Systems for 65. doi:10.4161/19420862.2014.985952. doi:10.1038/srep09756. Chemistry and Life Sciences (MicroTAS 2013), Freiburg, Germany, (26-28). 27-31 October, 2013. Lane, et al., (2015) Analysis of exosome Nguyen Tran Patents: Device and method for the detection purification methods using a model liposome Qualifications: Bachelor of Science (Materials of target entities system and tunable-resistive pulse sensing. Science) Scientific Reports, 5 7639.1-7639.7. doi:10.1038/ PhD Awarded: May srep07639. Michael Song Principal Supervisor: Professor Michael Vaidyanathan, et al., (2014) Tuneable surface Qualifications: Bachelor of Applied Science Monteiro shear forces to physically displace nonspecific (Biotechnology) - Queensland University Thesis Title: Synthesis of timed-release molecules in protein biomarker detection. of Technology, Master of Biotechnology - polymer nanoparticles Biosensors and Bioelectronics, 61 184-191. University of Queensland Publications: doi:10.1016/j.bios.2014.03.061. PhD Awarded: November Tran, et al., (2013) Fine tuning the Vaidyanathan, et al., (2014) Detecting Principal Supervisor: Professor Peter Gray disassembly time of thermoresponsive polymer exosomes specifically: a multiplexed Thesis Title: Novel Cell Engineering of the nanoparticles. Biomacromolecules, 14 10: 3463- device based on alternating current Unfolded Protein Response to Achieve Efficient 3471. doi:10.1021/bm4007858. electrohydrodynamic induced nanoshearing. Therapeutic Protein Production Cell Line Analytical Chemistry, 86 22: 11125-11132. Publications: doi:10.1021/ac502082b. Gillard, et al., (2014) Intracellular trafficking

AIBN Annual Report 2015 - 61 David Wibowo Williams, et al., (2015) Quorum-sensing polyethylenimine functionalized silica-based Qualifications: Bachelor of Science (Chemical linked RNA interference for dynamic metabolic spheres with small size for siRNA delivery. ACS Engineering) - Widya Mandala Catholic pathway control in Saccharomyces cerevisiae. Applied Materials and Interfaces, 6 18: 15626- University, Master of Science Engineering Metabolic Engineering, 29 124-134. doi:10.1016/j. 15631. doi:10.1021/am503060n. (Chemical Engineering) - National Taiwan ymben.2015.03.008. Yang, et al., (2014) Synthesis of SBA-15 rods University of Science and Technology Williams, et al., (2015) Dynamic regulation with small sizes for enhanced cellular uptake. PhD Awarded: May of gene expression using sucrose responsive Journal of Materials Chemistry B, 2 30: 4929- Principal Supervisor: Dr Chunxia Zhao promoters and RNA interference in 4934. doi:10.1039/c4tb00595c. Thesis Title: Bio-inspired oil-core silica- Saccharomyces cerevisiae. Microbial Cell Yu, et al., (2014) Facile synthesis of ultra- shell nanocapsules for controlled-release Factories, 14 43: 1-10. doi:10.1186/s12934-015- small hybrid silica spheres for enhanced applications 0223-7. penetration of 3D glioma spheroids. Chemical Publications: Williams, et al., (2013) Engineered quorum- Communications, 50 13: 1527-1529. doi:10.1039/ Wibowo, et al., (2015) Interfacial biomimetic sensing using pheromone-mediated cell-to-cell C3CC48416E. synthesis of silica nanocapsules using a communication in Saccharomyces cerevisiae. Yang, et al., (2014) Synthesis of silica vesicles recombinant catalytic modular protein. ACS Synthetic Biology, 2 3: 136-149. doi:10.1021/ with small sizes and reduced aggregation for Langmuir, 31 6: 1999-2007. doi:10.1021/ sb300110b. photodynamic therapy. Chemistry Letters, Article la504684g. in press . doi:10.1246/cl.130930. Wibowo, et al., (2014) Sustained release Yannan Yang of fipronil insecticide in Vitro and in Vivo from Qualifications: Bachelor of Science Tianyu Yang biocompatible silica nanocapsules. Journal of (Chemistry) - Wuhan University, Master of Qualifications: Bachelor of Science Agricultural and Food Chemistry, 62 52: 12504- Engineering (Engineering Science) - University (Biotechnology) - Northwest University 12511. doi:10.1021/jf504455x. of Queensland PhD Awarded: July Wibowo, et al., (2014) Emulsion-templated MPhil Awarded: March Principal Supervisor: Professor Michael silica nanocapsules formed using bio-inspired Principal Supervisor: Professor Chengzhong Monteiro silicification. Chemical Communications, 50 77: Yu Thesis Title: Design, Synthesis and 11325-11328. doi:10.1039/c4cc04904g. Thesis Title: Synthesis of Self-assembled Applications of Functional Cabonaceous Wibowo, et al., (2010) Nonleaching Nanoporous Silica for Therapeutic Delivery Nanospheres antimicrobial cotton fibers for hyaluronic acid Publications: Publications: adsorption. Biochemical Engineering Journal, 53 Zhang, et al., (2015) Self-Organized Yang, Tet al., (2015) Hierarchical mesoporous 1: 44-51. doi:10.1016/j.bej.2010.09.002. Mesostructured Hollow Carbon Nanoparticles yolk-shell structured carbonaceous Wibowo, et al., (2007) Adsorption of via a Surfactant-Free Sequential Heterogeneous nanospheres for high performance benzene and toluene from aqueous solutions Nucleation Pathway. Chemistry of Materials, electrochemical capacitive energy storage. onto activated carbon and its acid and 27 18: 6297-6304. doi:10.1021/acs. Chemical Communications, 51 13: 2518-2521. heat treated forms: Influence of surface chemmater.5b01993. doi:10.1039/c4cc09366f. chemistry on adsorption. Journal of Hazardous Niu, et al., (2015) Synthesis of silica Yang, et al., (2014) N-doped mesoporous Materials, 146 1-2: 237-242. doi:10.1016/j. nanoparticles with controllable surface carbon spheres as the oxygen reduction jhazmat.2006.12.011. roughness for therapeutic protein delivery. reaction catalysts. Journal of Materials Chemistry Patents: Silica Micro- and Nano-capsules Journal of Materials Chemistry B, 3 43: 8477- A, 2 42: 18139-18146. doi:10.1039/c4ta04301d. and methods for making them 8485. doi:10.1039/c5tb01405k. Yang, et al., (2013) Facile Fabrication Yang, et al., (2015) Preparation of of CoreShell-Structured Ag@Carbon and Thomas Williams fluorescent mesoporous hollow silica-fullerene Mesoporous YolkShell-Structured Ag@ Qualifications: Bachelor of Science nanoparticles via selective etching for combined Carbon@Silica by an Extended Stober Method. (Biochemistry) - University of Waikato, Master chemotherapy and photodynamic therapy. Chemistry-A European Journal, 19 22: 6942-6945. of Science - University of Waikato Nanoscale, 7 28: 11894-11898. doi:10.1039/ doi:10.1002/chem.201300523. PhD Awarded: March c5nr02769a. Principal Supervisor: Dr Claudia Vickers Yang, et al., (2015) Biphasic synthesis of Bijun Zeng Thesis Title: Separating Growth and large-pore and well-dispersed benzene bridged Qualifications: Bachelor of Biotechnology - Ji Production Phases Using Synthetic Quorum mesoporous organosilica nanoparticles for Nan University, Master of Biotechnology - Sensing in Saccharomyces cerevisiae intracellular protein delivery. Small, 11 23: 2743- University of Queensland Publications: 2749. doi:10.1002/smll.201402779. PhD Awarded: February Peng, et al., (2015) Controlling heterologous Zhang, et al., (2014) Nanodispersed UV Principal Supervisor: Professor Anton gene expression in yeast cell factories on blockers in skin-friendly silica vesicles with Middelberg different carbon substrates and across the superior UV-attenuating efficiency. Journal Thesis Title: Tailorable Nanocarrier Emulsion diauxic shift: a comparison of yeast promoter of Materials Chemistry B, 2 44: 7673-7678. for Drug Delivery activities. Microbial Cell Factories, 14 91: 1-11. doi:10.1039/c4tb01332h. Patents: Nanocarrier emulsions doi:10.1186/s12934-015-0278-5. Yu, et al., (2014) An approach to prepare

62 - AIBN Annual Report 2015 Jun Zhang mesoporous organosilica nanoparticles for doi:10.1021/ja402463h. Qualifications: Bachelor of Science (Chemistry) intracellular protein delivery. Small, 11 23: 2743- Yang, et al., (2013) Confinement of - Fudan University 2749. doi:10.1002/smll.201402779. chemisorbed phosphates in a controlled PhD Awarded: June Abbaraju, et al., (2014) Floating tablets nanospace with three-dimensional Principal Supervisor: Professor Chengzhong from mesoporous silica nanoparticles. Journal mesostructures. Chemistry-A European Yu of Materials Chemistry B, 2 47: 8298-8302. Journal, 19 18: 5578-5585. doi:10.1002/ Thesis Title: Synthesis of Novel Silica Vesicles doi:10.1039/c4tb01337a. chem.201300273. and Their Pharmaceutical and Cosmetic Mody, et al., (2014) Silica vesicles as Patents: Silica nanovesicles and vaccine Applications nanocarriers and adjuvants for generating both applications Publications: antibody and T-cell mediated immune resposes Mody, et al., (2015) Silica Vesicle to Bovine Viral Diarrhoea Virus E2 protein. Ruifeng Zhou Nanovaccine Formulations Stimulate Long- Biomaterials, 35 37: 9972-9983. doi:10.1016/j. Qualifications: Bachelor of Science (Physics) Term Immune Responses to the Bovine Viral biomaterials.2014.08.044. - Tsinghua University, Master of Science Diarrhoea Virus E2 Protein. PLoS One, 10 12: . Zhang, et al., (2014) Nanodispersed UV (Physics) - Tsinghua University doi:10.1371/journal.pone.0143507. blockers in skin-friendly silica vesicles with PhD Awarded: October Shen, et al., (2015) Nitrogen-doped ordered superior UV-attenuating efficiency. Journal Principal Supervisor: Dr Shizhang Qiao mesoporous carbon single crystals: Aqueous of Materials Chemistry B, 2 44: 7673-7678. Thesis Title: Preparation and Mechanism organic-organic self-assembly and superior doi:10.1039/c4tb01332h. Study of Non-precious Metal Catalysts for supercapacitor performance. Journal of Yang, et al., (2014) Synthesis of SBA-15 rods Electrochemical Oxygen Reduction Materials Chemistry A, 3 47: 24041-24048. with small sizes for enhanced cellular uptake. Publications: doi:10.1039/c5ta06129f. Journal of Materials Chemistry B, 2 30: 4929- Zhou, et al., (2015) An Fe/N co-doped Purwajanti, et al., (2015) Synthesis of 4934. doi:10.1039/c4tb00595c. graphitic carbon bulb for high-performance Magnesium Oxide Hierarchical Microspheres: A Lei, et al., (2014) Sensitive detection of oxygen reduction reaction. Chemical Dual-Functional Material for Water Remediation. human insulin using a designed combined pore Communications, 51 35: 7516-7519. doi:10.1039/ ACS Applied Materials and Interfaces, 7 38: 21278- approach. Small, 10 12: 2413-2418. doi:10.1002/ c5cc00995b. 21286. doi:10.1021/acsami.5b05553. smll.201303748. Yang, et al., (2015) Hierarchical mesoporous Zhang, et al., (2015) Self-Organized Qian, et al., (2014) A combo-pore approach yolk-shell structured carbonaceous Mesostructured Hollow Carbon Nanoparticles for the programmable extraction of peptides/ nanospheres for high performance via a Surfactant-Free Sequential Heterogeneous proteins. Nanoscale, 6 10: 5121-5125. electrochemical capacitive energy storage. Nucleation Pathway. Chemistry of Materials, doi:10.1039/c4nr00633j. Chemical Communications, 51 13: 2518-2521. 27 18: 6297-6304. doi:10.1021/acs. Popat, et al., (2014) Programmable drug doi:10.1039/c4cc09366f. chemmater.5b01993. release using bioresponsive mesoporous silica Yang, et al., (2014) N-doped mesoporous Niu, et al., (2015) Synthesis of silica nanoparticles for site-specific oral drug delivery. carbon spheres as the oxygen reduction nanoparticles with controllable surface Chemical Communications, 50 42: 5547-5550. reaction catalysts. Journal of Materials Chemistry roughness for therapeutic protein delivery. doi:10.1039/C4CC00620H. A, 2 42: 18139-18146. doi:10.1039/c4ta04301d. Journal of Materials Chemistry B, 3 43: 8477- Zhang, et al., (2014) Synthesis of silica Zhou, et al., (2014) Silver/nitrogen-doped 8485. doi:10.1039/c5tb01405k. vesicles with controlled entrance size for high graphene interaction and its effect on Ahmad, et al., (2015) Shaping nanoparticles loading, sustained release, and cellular delivery electrocatalytic oxygen reduction. Chemistry with hydrophilic compositions and hydrophobic of therapeutical proteins. Small, 10 24: 5068- of Materials, 26 20: 5868-5873. doi:10.1021/ properties as nanocarriers for antibiotic delivery. 5076. doi:10.1002/smll.201401538. cm502260m. ACS Central Science, 1 328-334. doi:10.1021/ Jarnbhrunkar, et al., 2013) Stepwise pore acscentsci.5b00199. size reduction of ordered nanoporous silica Yang, et al., (2015) Biphasic synthesis of materials at angstrom precision. Journal of the large-pore and well-dispersed benzene bridged American Chemical Society, 135 23: 8444-8447.

AIBN Annual Report 2015 - 63 Milestone student develops career

In 2015 Dr Nick Fletcher completed his PhD He then continued his studies at AIBN, Reflecting on his time as an AIBN student, Dr in the Whittaker Group, and in the process competing his Honours in the same laboratory Fletcher said he has fond memories. became the one hundredth AIBN graduate. In working on a diagnostic for tuberculosis under a phase of rapid growth, AIBN’s first research the supervision of Dr Ashley Connolly. “The AIBN has great equipment higher degree graduate completed their studies in 2008, making Dr Fletcher’s graduation only “The diagnostic itself really wasn’t successful in the labs, and being able to seven years later a remarkable achievement for at all, but the project was really working out access all of the facilities on the the Institute. why it didn’t work, and that is a valuable lesson,” he said. UQ campus is incredible; I don’t Now a Postdoctoral Researcher at UQ’s Centre know of anywhere else where for Advanced Imaging supervised by AIBN “We have students now who are worried that Associate Group Leader Associate Professor they are not getting positive results, but at you have so many capabilities in Kristofer Thurecht, he continues to have ties that stage of their development the focus is one place,” he said. with the Institute that date back to his time as not necessarily about making discoveries, but rather bringing up their technical skills and an undergraduate student. “I was part of the AIBN Student Association, learning to troubleshoot.” and it was a good experience to meet other Dr Fletcher began his studies at AIBN in 2008 students and find out more about them as In 2010 he began a PhD under the supervision as a third year undergraduate science student individuals with diverse backgrounds and of Dr Annette Dexter, Dr Kevin Jack and completing a project in Professor Matt Trau’s researchers working on a variety of projects.” laboratory under the supervision of Dr Darby Professor Andrew Whittaker. It was a joint project, firstly developing peptide bio- Kozak. The opportunities to develop his skills and surfactants as a greener alternative to the career have now led him to making polymer- petrochemical derived surfactants that are nanoparticles that specifically target breast “It was really valuable as an commonly used today. Secondly, it was cancer. undergraduate to see how discovered that some of the designer peptides he was using could form very soft, almost jelly- a laboratory actually works, like hydrogels. because it is completely “We studied whether these hydrogels could different to what you experience have applications in wound healing by acting in a science classroom,” Dr as a scaffold for regenerating tissue or nerves to reduce scar tissue or brain injury,” Dr Fletcher said. Fletcher said.

64 - AIBN Annual Report 2015 High achieving graduate takes on US challenge

Graduation from AIBN in late 2014 set off a chain of positive events for Dr Kewei Wang that have helped to firmly establish his career as a researcher.

Joining the Institute in 2011 from China, and having embraced the Australian way of life, Dr Wang’s work was focussed on the development of multifunctional polymers as contrast agents for molecular imaging.

He achieved his PhD award and was also proudly a recipient of the UQ Dean’s Award for Outstanding Research Higher Degree Theses.

Each year the Dean’s Award is granted to no more than 10 per cent of the theses submitted by graduating research higher degree students.

“I felt very surprised when I received the news, because I knew there were hundreds of other PhD graduates who had done as well as me,” Dr Wang said.

“I am glad and honoured to have been given this award.”

Dr Wang said that completing his PhD studies at AIBN has significantly shaped his career, which has landed him with a prestigious role at The University of Tennessee, Knoxville.

Now a Postdoctoral Research Associate in the Department of Chemistry, he is working on a project funded by the U.S. Department of Energy on the development of hybrid nanoparticles as new lubricant additives to improve fuel efficiency.

“UTK is one of the oldest universities in this “The knowledge and findings I gained from country, and has a renowned reputation for “In addition, my wonderful my PhD work were mainly the fundamental outstanding teaching and research,” Dr Wang understanding and design concept for 19F supervisors, Professor Andrew said. MRI-based theranostic agents,” he said. Whittaker, Associate Professor “In the past few months I have “As cancer diseases have become a leading Kris Thurecht and Dr Hui Peng, been enjoying my work and cause of death in the developed countries, gave me the chance to work in our hope is to develop polymeric multimodal life in this beautiful place with imaging agents for early and accurate cancer an exciting research field and a long history, and I think that diagnosis, which is vital for successful guided me in conducting high- treatment.” working at UTK is an exciting quality research with a creative beginning of my professional Dr Wang praises the facilities offered to him at and critical attitude.” AIBN as world-class enablers of his research. career.” This solid foundation has provided Dr Wang the “The access to modern equipment and facilities opportunity to develop his career as an early Although he misses the people and natural provided me with essential skills to work career researcher, which he has taken with both environment of Australia, Dr Wang hopes he efficiently and safely in a modern research hands. left his own mark through his work as a PhD environment,” he said. student in the Whittaker Group.

AIBN Annual Report 2015 - 65 Engagement

66 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 67 Scientific engagement expands AIBN’s reach

Each year AIBN researchers are involved in Internationally, Professor Andrew Whittaker’s distinct pluripotent states, and Professor local, national and international professional long-term efforts in developing links with Philippe Collas of the University of Oslo, engagement and outreach activities. Their China were rewarded with the establishment Norway, presented on cloning and induced efforts raise the scientific profile and of the Wuhan-Brisbane Research Alliance in pluripotent stem cells. awareness of the work undertaken at AIBN, Functional Polymeric Materials. and help to establish valuable research The conference featured 11 invited speakers, collaborations. The alliance ties AIBN and the Queensland as well as presentations from students and University of Technology with five Chinese early career researchers. institutions which include Wuhan Institute In December AIBN Group of Technology, Wuhan University, Huazhong On 27 November AIBN researchers Associate Leader and Inaugural Director University of Science and Technology, Hubei Professor Stephen Mahler and Dr Simon Corrie University, and the Chinese Academy of co-organised the Biomedical Applications of Professor Peter Gray was Sciences Institute of Intelligent Machines. Engineered Antibodies and Proteins workshop appointed as Interim President Professor Whittaker will manage the Brisbane held in UQ’s Advanced Engineering Building. of the Australian Academy of node of the alliance. The workshop featured a Technological Sciences and 2015 also saw a number of high profile events Engineering (ATSE). organised by researchers at the Institute. number of speakers from The 3rd Annual Cell Reprogramming Australia Australian academic and He replaced outgoing President Dr Alan Finkel Conference 2015 was held at AIBN over 11-12 AO, who was appointed as Australia’s Chief research institutions, as well May, and was organised by the StemCore Scientist by Prime Minister Malcolm Turnbull. facility, which holds AIBN researchers on its as speakers from industry. It Board of Directors and Scientific Advisory ATSE is one of four learned Academies in was focussed on the field of Committee. Australia; advocating for technological and antibody engineering, and the scientific development for social, environmental Opened by AIBN Group Leader Professor and economic benefit, it has more than 800 latest developments on how it Ernst Wolvetang, the conference featured a inducted Fellows within its ranks. presentation by fellow AIBN Group Leader can be applied to applications Professor Christine Wells and plenary lectures Professor Gray had been on the ATSE board for such as theranostics, reagents, from two international speakers. six years, including serving as Vice President, biologic medicines and targeted and was inducted as a Fellow in 1992. Dr Jacob Hanna of the Weizmann Institute nanomedicines. of Science, Israel, spoke on the molecular mechanisms for assembling and resolving

68 - AIBN Annual Report 2015 Each year AIBN researchers are involved in a number of engagements which further the Institute’s work to their scientific peers. Their efforts increase the scientific profile of the work undertaken at AIBN, and regularly lead to research collaborations.

AIBN researchers spoke during the The event saw ECRs from 14 life science The AIBN Student Association also provided proceedings, with Professor Matt Trau institutes and schools across Brisbane valuable experience for young researchers discussing cell membrane antibody fragments give oral and poster presentations. Oral to present their work in a formal setting, as a new class of affinity agents, and Dr presentations were made by AIBN’s Dr David hosting the annual AIBN Student Conference Chris Howard presenting on the development Wibowo and Dr Chris Howard, and poster at Customs House on 2 October. The event of cancer targeted nanomaterials that use presentations were made by 10 AIBN ECRs. featured oral and digital poster presentations bispecific antibodies. by more than 30 AIBN students.

The Centre for Theoretical and Computational Molecular Science at AIBN led by Professor Debra Bernhardt organised a Student and ECR Mini Symposium on 21 May for researchers in that field.

The event featured a presentation by guest speaker Dr David Huang of University of Adelaide, who spoke about designing and identifying porous molecular materials for gas storage and separation.

Other presentations were made by student and ECR speakers, together with a poster presentation that was exclusively for PhD students to display their research.

AIBN’s Early-Mid Career Researcher Committee was actively involved in the organisation of the 6th Annual ECR Symposium on 4 December at UQ’s Advanced Engineering Building.

AIBN Annual Report 2015 - 69 Community and school engagement activities

AIBN staff and students actively partake in Australia to discuss the topic of translating UQ, Cracking the Code, detailing the story of broad community and school engagement stem cell research into real health and Stephen and Sally Damiani and their search to activities to raise awareness of not only the economic benefits. identify a diagnosis for their ill son, Massimo. work directly undertaken at the Institute, but also to promote the importance and value of The panel debated the potential benefits Genetic sequencing discovered a previously scientific advances. of creating a personal body repair kit, the unknown form of childhood leukodystrophy, technical challenges that remain to be collared, and the family has established the Mission Through media appearances, school visits and and considered the potential ethical questions Massimo Foundation. This has provided community consultations, AIBN researchers raised by stem cell based therapies. financial support for ongoing research within are able to provide insights into their work, the Professor Ernst Wolvetang’s laboratory to current scientific landscape, and offer views of A further initiative undertaken during Stem Cell search for treatments for the disease. how their fields may positively impact society Week was the StemCells@UQ event held at in the future. AIBN on 11 May for secondary school students AIBN research by Professor Darren Martin’s in grades 9 and 10, which provided the group into spinifex grass also took science students with an opportunity to meet UQ stem interstate and into the gallery. A premier example of cell researchers. AIBN’s efforts to raise public The 24 September – 6 November exhibition Students engaged with a panel of experts, Ochre, Spinifex & Foil held at the Tin Sheds awareness during 2015 addressing how stem cell science has Gallery, The University of Sydney, investigated occurred during Stem Cell Week developed and how stem cells may impact Indigenous and non-Indigenous science, with people’s lives in the future, in particular the the Martin Group contributing through their (11-17 May), with a number of realities behind the hopes and fears associated research into nanocellulose derived from events being held and attended with stem cell science. spinifex.

by AIBN researchers. The event coincided with the 3rd Annual Cell Throughout the year AIBN researchers also Reprogramming Australia Conference, which made media appearances in leading outlets Professors Peter Gray, Ernst Wolvetang and was also held at AIBN. across television, radio, newspapers and online Christine Wells took part in UQ’s premier public platforms. lecture platform, the Global Leadership Series, During the year AIBN hosted other school on 14 May at Customs House, Brisbane. visits, which involved tours of the facilities Media coverage provides an opportunity for and laboratory demonstrations by AIBN researchers to inform and educate the broader As part of a panel moderated by ABC researchers. community about their own discoveries and broadcaster Dr Norman Swan, they joined findings, as well as provide commentary on Professor Alan Trounson of Monash University Further examples of community engagement their fields of expertise. and Professor Martin Pera of Stem Cells included the 1 April launch of a new book at

70 - AIBN Annual Report 2015 AIBN Seminar Series

Each year AIBN hosts a number of seminars from both domestic and international guest speakers to promote novel science to the Institute’s staff and students, fostering new ideas and collaborations.

23 February: Professor Ryan M. Richards, Department 3 July: Professor Wey Yang Teoh, Clean Energy 18 September: Professor Rob Capon, Institute for of Chemistry and Geochemistry, Materials Science, and Nanotechnology Lab, School of Energy and Molecular Bioscience, UQ Colorado School of Mines Joint Appointment at Environment, City University of Hong Kong Biodiscovery: In search of new Drugs to Treat National Renewable Energy Laboratory Heterojunction Engineering and Charge Transport in Infectious Diseases, Cancer and Pain Nanostructured Materials with Unique Physical and Photoelectrochemical Water Splitting Chemical Properties 25 September: Dr Matthew Kearnes, University of New 10 July: Professor Mitchell A. Winnik, Department of South Wales 13 March: Professor Christine Wells, AIBN, UQ Chemistry, University of Toronto, Canada NanoScience: Social Science Perspectives Systems Biology of Stem Cells One-Dimensional Block Copolymer Micelles and Their Hybrid Structures with Inorganic nanoparticles 6 October: Professor Jiming Hu, Institute of Analytical 20 March: Mr David Martin, Research Connections Biomedicine, Wuhan University, China Facilitator, AusIndustry 13 July: Professor Leaf Huang, University of North Bio-Raman Spectroscopy: A Bridge between Shaping industry with your Research – Let us show Carolina at Chapel Hill, USA Pathological Research and Clinical Diagnosis of you how! Lipid-Stabilized Calcium Phosphate Nanoparticles for Disease Gene and Drug Delivery 27 March: Dr Esteban Marcellin, Associate Group 9 October: Professor Nancy Monteiro-Riviere, Director Leader, AIBN, UQ 17 July: Professor Jürgen Götz, Foundation Chair of the Nanotechnology Innovation Center, Kansas Systems Metabolic Engineering for Industrial Process of Dementia Research; Director, Clem Jones Centre State University (NICKS), USA Improvements for Ageing Dementia Research, Queensland Brain Physicochemical Properties of Nanoparticles that Institute, UQ may Influence Skin Penetration and Toxicity 13 April: Professor Jayakrishnan, Department of Lessons from Alzheimer’s Disease Models - Novel Biotechnology, Bhupat and Jyothi Mehta School of Approaches to Remove Toxic Protein Aggregates from 9 October: Professor Jim Riviere, Director of the Biosciences, Indian Institute of Technology the Brain Institute of Computational Comparative Medicine, Polymeric Prodrugs of Amphotericin B and Kansas State University, USA Primaquine 24 July: Dr David Noon FTSE, Vice President of Global Development of a Physiologically Relevant Operations & Sales, GroundProbe Pty Ltd Multivariate Nanoparticle Molecular Interaction 16 April: Adjunct Professor Jens Sommer-Knudsen, Industry-focused Research: A Career Pathway Fingerprint Endoluminal Sciences Towards Innovation and Entrepreneurship A novel Protein NanoParticle Technology 16 October: Dr Barbara Rolfe, AIBN, UQ 31 July: Professor Bernd H. A. Rehm, Institute of The Complement Anaphylatoxins: Therapeutic Targets 17 April: Professor Jan Recker, Queensland University Fundamental Sciences and MacDiarmid Institute for for Melanoma? of Technology Advanced Materials and Nanotechnology, Massey Collaborate to Innovate: Lessons Learnt from Three University, New Zealand 21 October: Professor Yasuhiro Sakamoto, Years of the Woolworths Chair of Retail Innovation In vivo Assembly of Functionalised Shell-core Department of Physics, Osaka University, Japan Nanobeads by Engineered Bacteria Electron Microscopy Studies of Nanoporous Materials 17 April: Professor Samuel S. Mao, University of on the Micro- and Meso-scale California at Berkeley, USA 10 August: Dr Roland Marshall, Justus-Liebig- Disorder-Engineered Titanium Dioxide Nanocrystals: University Giessen, Germany 23 October: Professor Chris Goodnow, Deputy Fundamentals and Application to Solar-Driven Mesostructured Mixed Oxides for Solar Energy Director, The Bill and Patricia Ritchie Foundation, Hydrogen Production Conversion Garvan Institute of Medical Research Analysis of Mutations in Antibodies, Cancer, and Germ 23 April: Dr Noel Chambers, Chief Executive Officer, 21 August: Dr Esther Levy, Consulting Editor, Line Genome National Foundation for Medical Research and Advanced Materials, Wiley, Australia Innovation (NFMRI) Materials Science Publishing: An Advanced 30 October: Professor Takayoshi Sasaki, National A New Approach to Philanthropy Supporting Medical Perspective Institute for Materials Sciences, Japan Research and Innovation 2D Nanoarchitectonics with Metal Oxide and 21 August: Professor Christoph Hagemeyer, Head, Hydroxide Nanosheets 24 April: Professor Xuchuan Jiang, Laboratory for Vascular Biotechnology Laboratory, Baker IDI Simulation and Modelling of Particulate Systems Molecular Imaging and Targeted Drug Delivery for the 30 October: Professor Shuming Nie, Department of (SIMPAS), Department of Chemical Engineering, Prevention, Diagnosis and Treatment of Myocardial Biomedical Engineering, Emory University and Georgia Monash University Infarction and Stroke Institute of Technology, USA Engineering Nanoparticles and their Nanocoatings for Nanotechnology and Precision Medicine: Image- Energy and Environmental Applications 28 August: Professor Christopher J.H. Porter, Guided Surgical Resection of Microscopic Tumors Professor of Pharmaceutics and Associate Dean 8 May: Associate Professor Mark Molloy, APAF (Research), Monash Institute of Pharmaceutical 26 November: Kuan-Lin (Elly) Ku, National Tsing Hua Director, Australian Proteome Analysis Facility (APAF) Sciences University, Taiwan A Protein-Centric View of Cancer Signalling: Therapies Dendrimers as Enhanced Drug Delivery Vectors Development of the Potential of Polyanhydride and Biomarkers Copolymers and Hydroxyapatite Ceramic Composites 3 September: Dr Steve D R Christie, Senior Lecturer for Bone Applications 15 May: Professor Geoffrey Faulkner, Translational and Associate Dean (Enterprise) of the Department of Research Institute Chemistry, Loughborough University, UK 30 November: Prof I-Ming Chu, Chemical Engineering Mobile DNA: Shuffling the Genetic Deck in Cancer, The Additive Manufacturing of Chemistry and Biology: Department, National Tsing Hua University, Hsinchu, Stem Cells and Neurons Towards 3D Printing of Science? Taiwan Biodegradable Thermo-Sensitive Polymeric Hydrogels 22 May: Professor Julian Rood, Monash University 11 September: Professor Brett Neilan, School of and Their Biomedical Applications NetB Toxin and Avian Necrotic Enteritis: The Hole Biotechnology and Biomolecular Sciences, University Movable Story of New South Wales 7 December: Dr Jeffrey Wiggins, University of Engineering Cyanobacteria and their Toxin Southern Mississippi, USA 29 May: Dr Timothy Mercer, Garvan Institute of Biosynthesis Pathways for Unnatural Production Next Generation Aerospace Composite Materials Medical Research Genes Under the Microscope: Targeted Sequencing of 14 September: Chris Stowers, Fermentation 10 December: Dr Lionel Hebbard, Department of the Human Transcriptome Technology Leader, Bioengineering and Bioprocess Molecular and Cell Biology, James Cook University R&D, Dow AgroSciences LLC Adiponectin and its Role in Human Disease The Challenges of Industrial Fermentation – From Primary to Secondary Metabolites

AIBN Annual Report 2015 - 71 Publications

72 - AIBN Annual Report 2015 AIBN Annual Report 2015 - 73 Publications

Book Chapter Ali, N., Jurczyluk, J., Shay, G., Tnimov, Z., 7.11% through simultaneous engineering of Alexandrov, K., Munoz, M. A., Skinner, O. P., photocathode and photoanode, Nano Energy Annamalai, P. K., and Depan, D. (2015) Nano- Pavlos, N. J., and Rogers, M. J. (2015) A 13, 609-619. cellulose reinforced chitosan nanocomposites highly sensitive prenylation assay reveals in for packaging and biomedical applications, vivo effects of bisphosphonate drug on the Banks, T. A., Luckman, P. S. B., Frith, J. E., and in Green biorenewable biocomposites: from Rab prenylome of macrophages outside the Cooper-White, J. J. (2015) Effects of electric knowledge to industrial applications (Vijay skeleton, Small GTPases 6. fields on human mesenchymal stem cell Kumar, T., and Michael, R. K., Eds.), pp 489-506, behaviour and morphology using a novel Apple Academic Press, Oakville, ON, Canada. Alosime, E. M., Edwards, G. A., and Martin, multichannel device, Integrative Biology (United D. J. (2015) Structure-property relationships Kingdom) 7, 693-712. Fay, M., Bell, C., Dowson, N., Puttick, S., and in copolyester elastomer-layered silicate Rose, S. (2015) Imaging of brain tumours, in nanocomposites, Journal of Applied Polymer Barnabas, M. J., Parambadath, S., Mathew, A., Molecular considerations and evolving surgical Science 132, 41742, doi: 41710.41002/ Park, S. S., Vinu, A., and Ha, C.-S. (2016) Highly management issues in the treatment of patients app.41742. efficient and selective adsorption of In3+ on with a brain tumor (Dr. Terry, L., Ed.), pp 235- pristine Zn/Al layered double hydroxide (Zn/Al- 276, InTech Europe, Rijeka, Croatia. Amiralian, N., Annamalai, P. K., Memmott, P., LDH) from aqueous solutions, Journal of Solid and Martin, D. J. (2015) Isolation of cellulose State Chemistry 233, 133-142. Gu, W., McMillan, N., and Yu, C. (2015) Silencing nanofibrils from Triodia pungens via different of E6/E7 expression in cervical cancer stem-like mechanical methods, Cellulose 22, 2483-2498. Becherel, O. J., Sun, J., Yeo, A. J., Nayler, S., cells, in Cervical cancer: methods and protocols Fogel, B. L., Gao, F., Coppola, G., Criscuolo, C., (Daniel, K., and Athena, W. L., Eds.), pp 173-182, Amiralian, N., Annamalai, P. K., Memmott, P., De Michele, G., Wolvetang, E., and Lavin, M. F. Springer, New York, NY United States. Taran, E., Schmidt, S., and Martin, D. J. (2015) (2015) A new model to study neurodegeneration Easily deconstructed, high aspect ratio cellulose in ataxia oculomotor apraxia type 2, Human Osborne, G. W. (2015) A brief introduction nanofibres from Triodia pungens; an abundant Molecular Genetics 24, 5759-5774. to neural flow cytometry from a practical grass of Australia’s arid zone, RSC Advances 5, perspective, in Neural surface antigens: from 32124-32132. Behrendorff, J. B. Y. H., Huang, W., and Gillam, E. basic biology towards biomedical applications M. J. (2015) Directed evolution of cytochrome (Jan, P., Ed.), pp 15-26, Elsevier, London, United Anderson, W., Lane, R., Korbie, D., and Trau, P450 enzymes for biocatalysis: exploiting the Kingdom. M. (2015) Observations of tunable resistive catalytic versatility of enzymes with relaxed pulse sensing for exosome analysis: improving substrate specificity, Biochemical Journal 467, Thakar, N. Y., and Wolvetang, E. J. (2015) system sensitivity and stability, Langmuir 31, 1-15. Ascorbate as a modulator of the epigenome, 6577-6587. in Nutrition and epigenetics (Emily, H., and Bell, C., Dowson, N., Fay, M., Thomas, P., Puttick, Frederick, D., Eds.), pp 199-218, CRC Press, Arner, E., Beckhouse, A., Briggs, J., Ovchinnikov, S., Gal, Y., and Rose, S. (2015) Hypoxia imaging Boca Raton, FL, United States. D., Wolvetang, E., Wells, C., and Consortium, in gliomas with 18F-fluoromisonidazole PET: F. (2015) Transcribed enhancers lead waves toward clinical translation, Seminars in Nuclear Vickers, C. E., Behrendorff, J. B. Y. H., Bongers, of coordinated transcription in transitioning Medicine 45, 136-150. M., Brennan, T. C. R., Bruschi, M., and Nielsen, mammalian cells, Science 347, 1010-1014. L. K. (2015) Production of industrially-relevant Bell, C., Dowson, N., Puttick, S., Gal, Y., Thomas, isoprenoid compounds in engineered microbes, Atanda, L., Mukundan, S., Shrotri, A., Ma, Q., P., Fay, M., Smith, J., and Rose, S. (2015) in Microorganisms in biorefineries (Birgit, K., and Beltramini, J. (2015) Catalytic conversion Increasing feasibility and utility of 18F-FDOPA Ed.), pp 303-334, Springer, Berlin, Heidelberg. of glucose to 5-hydroxymethyl-furfural with a PET for the management of glioma, Nuclear phosphated TiO2 catalyst, ChemCatChem 7, Medicine and Biology 42, 788-795. Zong, X., Lu, G., and Wang, L. (2015) 781-790. Nonmetal doping in TiO2 toward visible-light- Bell, C. A., Hedir, G. G., O’Reilly, R. K., and induced photocatalysis, in Environmental Atanda, L., Shrotri, A., Mukundan, S., Ma, Q., Dove, A. P. (2015) Controlling the synthesis Photochemistry Part III (Detlef, W. B., and Konarova, M., and Beltramini, J. (2015) Direct of degradable vinyl polymers by xanthate- Peter, K. J. R., Eds.), pp 87-113, Springer, Berlin, production of 5-Hydroxymethylfurfural via mediated polymerization, Polymer Chemistry 6, Germany. catalytic conversion of simple and complex 7447-7454. sugars over phosphated TiO2, ChemSusChem 8, 2907-2916. Bennett, T. M., Jack, K. S., Thurecht, K. J., Journal Article and Blakey, I. (2016) Perturbation of the Atanda, L., Silahua, A., Mukundan, S., Shrotri, experimental phase diagram of a diblock Abidin, R. S., Lua, L. H. L., Middelberg, A. P. J., A., Torres-Torres, G., and Beltramini, J. (2015) copolymer by blending with an ionic liquid, and Sainsbury, F. (2015) Insert engineering and Catalytic behaviour of TiO2-ZrO2 binary oxide Macromolecules 49, 205-214. solubility screening improves recovery of virus- synthesized by sol-gel process for glucose like particle subunits displaying hydrophobic conversion to 5-hydroxymethylfurfural, RSC Benson, M. J., Manzanero, S., and Borges, K. epitopes, Protein Science 24, 1820-1828. Advances 5, 80346-80352. (2015) Complex alterations in microglial M1/M2 markers during the development of epilepsy in Adelnia, H., Gavgani, J. N., and Babur, B. K., Futrega, K., Lott, W. B., Klein, T. two mouse models, Epilepsia 56, 895-905. Soheilmoghaddam, M. (2015) Fabrication of J., Cooper-White, J., and Doran, M. R. (2015) composite polymer particles by stabilizer-free High-throughput bone and cartilage micropellet Benson, M. J., Thomas, N. K., Talwar, S., Hodson, seeded polymerization, Colloid and Polymer manufacture, followed by assembly of M. P., Lynch, J. W., Woodruff, T. M., and Borges, Science 293, 2445-2450. micropellets into biphasic osteochondral tissue, K. (2015) A novel anticonvulsant mechanism via inhibition of complement receptor C5ar1 Ahmad Nor, Y., Niu, Y., Karmakar, S., Zhou, L., Cell and Tissue Research 361, 755-768. in murine epilepsy models, Neurobiology of Xu, C., Zhang, J., Zhang, H., Yu, M., Mahony, Bai, Y., Butburee, T., Yu, H., Li, Z., Amal, R., G.Q., Disease 76, 87-97. D., Mitter, N., Cooper, M., and Yu, C. Z. (2015) L., and Wang, L. (2015) Controllable synthesis of Shaping nanoparticles with hydrophilic concave cubic gold core-shell nanoparticles for Bernardi, S., and Searles, D. J. (2015) Local compositions and hydrophobic properties as plasmon-enhanced photon harvesting, Journal response in nanopores, Molecular Simulation, nanocarriers for antibiotic delivery, ACS Central of Colloid And Interface Science 449, 246-251. DOI: 10.1080/08927022.08922015.01049174. Science 1, 328-334. Bai, Y., Han, C., Chen, X., Yu, H., Zong, X., Li, Z., Bobrin, V. A., and Monteiro, M. J. (2015) Alateeq, S., Fortuna, P. R. J., and Wolvetang, and Wang, L. (2015) Boosting the efficiency Temperature-Directed Self-Assembly of E. (2015) Advances in reprogramming to of quantum dot sensitized solar cells up to Multifunctional Polymeric Tadpoles, Journal of pluripotency, Current Stem Cell Research and the American Chemical Society 137, 15652- Therapy 10, 193-207. 15655.

74 - AIBN Annual Report 2015 Boehm, M. W., Shewan, H. M., Steen, J. A., Chen, H., Liu, G., and Wang, L. (2015) Switched conditions on the membrane distillation and Stokes, J. R. (2015) Illustrating ultra-low- photocurrent direction in Au/TiO2 bilayer thin performance for desalination, Desalination 370, volume rheology on a conventional rheometer: films, Scientific Reports 5, 10852. 53-62. Charting the development of hyaluronan during fermentation, Applied Rheology 25, 12-19. Chen, H., Sun, J., Wolvetang, E., and Cooper- Chua, Y. T., Ji, G., Birkett, G., Lin, C. X. C., Kleitz, F., White, J. (2015) High-throughput, deterministic and Smart, S. (2015) Nanoporous organosilica Bongers, M., Chrysanthopoulos, P. K., single cell trapping and long-term clonal cell membrane for water desalination: theoretical Behrendorff, J. B. Y. H., Hodson, M. P., Vickers, culture in microfluidic devices, Lab on a Chip 15, study on the water transport, Journal of C. E., and Nielsen, L. K. (2015) Systems analysis 1072-1083. Membrane Science 482, 56-66. of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress Chen, H., Wang, Q., Lyu, M., Zhang, Z., and Wang, Chua, Y. T., Lin, C. X. C., Kleitz, F., and Smart, S. and the validity of lycopene as an isoprenoid L. (2015) Wavelength-switchable photocurrent (2015) Synthesis of mesoporous carbon-silica reporter metabolite, Microbial Cell Factories 14, in a hybrid TiO2-Ag nanocluster photoelectrode, nanocomposite water-treatment membranes 1-16. Chemical Communications 51, 12072-12075. using a triconstituent co-assembly method, Journal of Materials Chemistry A 3, 10480- Bose, U., Hewavitharana, A. K., Ng, Y. K., Shaw, Chen, J. (2015) Signaling pathways in 10491. P. N., Fuerst, J. A., and Hodson, M. P. (2015) HPV-associated cancers and therapeutic LC-MS-Based Metabolomics Study of Marine implications, Reviews in Medical Virology 25, Clark, M. B., Mercer, T. R., Bussotti, G., Leonardi, Bacterial Secondary Metabolite and Antibiotic 24-53. T., Haynes, K. R., Crawford, J., Brunck, M. E., Cao, K.-A. L., Thomas, G. P., Chen, W. Y., Taft, R. J., Production in Salinispora arenicola, Marine Chen, J., Gu, W., Yang, L., Chen, C., Shao, R., Xu, Drugs 13, 249-266. Nielsen, L. K., Enright, A. J., Mattick, J. S., and K., and Xu, Z. P. (2015) Nanotechnology in the Dinger, M. E. (2015) Quantitative gene profiling Brennan, T. C. R., Williams, T. C., Schulz, B. management of cervical cancer, Reviews in of long noncoding RNAs with targeted RNA L., Palfreyman, R. W., Kroemer, J. O., and Medical Virology 25, 72-83. sequencing, Nature Methods 12, 339-342. Nielsen, L. K. (2015) Evolutionary engineering Chen, X. Q., Li, Z., Bai, Y., Sun, Q., Wang, L. improves tolerance for replacement jet fuels Clulow, A. J., Cavaye, H., Tang, G., Shaw, P. E., Z., and Dou, S. X. (2015) Room-temperature Cooper-White, J. J., Burn, P. L., and Meredith, P. in Saccharomyces cerevisiae, Applied and synthesis of Cu2-xE (E = S, Se) nanotubes with Environmental Microbiology 81, 3316-3325. (2015) Quantitative real time sensing reveals hierarchical architecture as high-performance enhanced sensitivity of polar dendrimer thin Briggs, J. A., Wolvetang, E. J., Mattick, J. S., counter electrodes of quantum-dot-sensitized films for plastic explosive taggants, Journal of Rinn, J. L., and Barry, G. (2015) Mechanisms of solar cells, Chemistry - A European Journal 21, Materials Chemistry C 3, 9412-9424. long non-coding RNAs in mammalian nervous 1055-1063. Cork, J., Whittaker, A. K., and Cooper-White, system development, plasticity, disease, and Chen, Y., Tan, C., Zhang, H., and Wang, L. (2015) evolution, Neuron 88, 861-877. J. J. (2015) Tensile properties and in vitro Two-dimensional graphene analogues for degradation of P(TMC-co-LLA) elastomers, Catrice, E. V. B., and Sainsbury, F. (2015) biomedical applications, Chemical Society Journal of Materials Chemistry B 3, 4406-4416. Assembly and purification of polyomavirus-like Reviews 44, 2681-2701. Corrie, S. R., Coffey, J. W., Islam, J., Markey, K. particles from plants, Molecular Biotechnology Choi, G., Yang, J.-H., Park, G.-Y., Vinu, A., 57, 904-913. A., and Kendall, M. A. F. (2015) Blood, sweat, Elzatahry, A., Yo, C. H., and Choy, J.-H. (2015) and tears: developing clinically relevant protein Chen, A., Blakey, I., Jack, K. S., Whittaker, Intercalative ion-exchange route to amino acid biosensors for integrated body fluid analysis, A. K., and Peng, H. (2015) Control through layered double hydroxide nanohybrids and Analyst 140, 4350-4364. monomer placement of surface properties and their sorption properties, European Journal of morphology of fluoromethacrylate copolymers, Inorganic Chemistry, 925-930. Cosson, S., Otte, E. A., Hezaveh, H., and Cooper-White, J. J. (2015) Concise review: Journal of Polymer Science, Part A: Polymer Chua, Y., Lin, C. X. C., Kleitz, F., and Smart, S. Chemistry 53, 2633-2641. tailoring bioengineered scaffolds for stem (2015) Mesoporous organosilica membranes: cell applications in tissue engineering and Effects of pore geometry and calcination regenerative medicine, Stem Cells Translational Medicine 4, 156-164.

AIBN Annual Report 2015 - 75 Cui, Z., Stein, V., Tnimov, Z., Mureev, S., and Dong, H., Parekh, H. S., and Xu, Z. P. (2015) Emadzadeh, D., Lau, W. J., Rahbari-Sisakht, Alexandrov, K. (2015) Semisynthetic tRNA Particle size- and number-dependent delivery to M., Ilbeygi, H., Rana, D., Matsuura, T., and complement mediates in vitro protein synthesis, cells by layered double hydroxide nanoparticles, Ismail, A. F. (2015) Synthesis, modification and Journal of the American Chemical Society 137, Journal of Colloid And Interface Science 437, optimization of titanate nanotubes-polyamide 4404-4413. 10-16. thin film nanocomposite (TFN) membrane for forward osmosis (FO) application, Chemical Dai Pre, M., Martucci, A., Martin, D. J., Lavina, Du, Y., Kopittke, P. M., Noller, B. N., James, S. A., Engineering Journal 281, 243-251. S., and Di Noto, V. (2015) Structural features, Harris, H. H., Xu, Z. P., Li, P., Mulligan, D. R., and properties, and relaxations of PMMA-ZnO Huang, L. (2015) In situ analysis of foliar zinc Faghihi, E. M., Martin, D., Clifford, S., Edwards, nanocomposite, Journal of Materials Science absorption and short-distance movement in G., He, C., Asbach, C., and Morawska, L. (2015) 50, 2218-2228. fresh and hydrated leaves of tomato and citrus Are there generalizable trends in the release using synchrotron-based X-ray fluorescence of airborne synthetic clay nanoparticles from Davidson, K. C., Mason, E. A., and Pera, M. F. microscopy, Annals of Botany 115, 41-53. a jet milling process?, Aerosol and Air Quality (2015) The pluripotent state in mouse and Research 15, 365-375. human, Development (Cambridge) 142, 3090- Du, Y., Li, P., Nguyen, A. V., Xu, Z. P., Mulligan, 3099. D., and Huang, L. (2015) Zinc uptake and Farandos, N. M., Yetisen, A. K., Monteiro, M. distribution in tomato plants in response to J., Lowe, C. R., and Yun, S. H. (2015) Contact de Oliveira Dal’molin, C. G., Quek, L.-E., Saa, foliar supply of Zn hydroxide-nitrate nanocrystal lens sensors in ocular diagnostics, Advanced P. A., and Nielsen, L. K. (2015) A multi-tissue suspension with controlled Zn solubility, Journal Healthcare Materials 4, 792-810. genome-scale metabolic modelling framework of Plant Nutrition and Soil Science 178, 722- for the analysis of whole plant systems, 731. Fatima, B., Jabeen, F., Padashbarmchi, Z., and Frontiers in Plant Science 6, 4.1-4.12. Najam-Ul-Haq, M. (2015) Enhanced enrichment Dürig, W., Blakey, I., Grant, S., Chambers, L., performance of nickel oxide nanoparticles via Dhawale, D. S., Kim, S., Park, D.-H., Choy, J.-H., Escher, B. I., Weijs, L., and Gaus, C. (2015) fabrication of a nanocomposite with a graphene Al-deyab, S. S., Ariga, K., Kim, E., and Vinu, A. New polymer passive sampler for sensitive template, RSC Advances 5, 23658-23665. 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76 - AIBN Annual Report 2015 Fiset, E., Rufford, T. E., Seredych, M., Bandosz, degree of sulphonation in microbial fuel cell: Guo, L., Feng, W., Liu, X., Lin, C., Li, B., and Qiang, T. J., and Hulicova-Jurcakova, D. (2015) Application study and economical analysis, Y. (2015) Sol-gel synthesis of antibacterial Comparison of melamine resin and melamine International Journal of Hydrogen Energy, hybrid coatings on titanium, Materials Letters network as precursors for carbon electrodes, doi:10.1016/j.ijhydene.2015.1010.1029. 160, 448-451. Carbon 81, 239-250. Gillies, P. J., Bray, L. J., Richardson, N. A., Chirila, Guo, Y., Hill, D. J. T., Whittaker, A. K., Jack, K. Francis, G., and Stein, S. (2015) Circulating cell- T. V., and Harkin, D. G. (2015) Isolation of S., and Peng, H. 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AIBN Annual Report 2015 - 77 Hankel, M., Ye, D., Wang, L., and Searles, D. J. Hedir, G. G., Bell, C. A., O’Reilly, R. K., and Dove, Huynh, H. T., Tran, T. T. B., Chan, L. C. L., Nielsen, (2015) Lithium and sodium storage on graphitic A. P. (2015) Functional degradable polymers L. K., and Reid, S. (2015) Decline in Helicoverpa carbon nitride, Journal of Physical Chemistry C by radical ring-opening copolymerization of armigera nucleopolyhedrovirus occlusion body 119, 21921-21927. MDO and vinyl bromobutanoate: synthesis, yields with increasing infection cell density in degradability and post-polymerization vitro is strongly correlated with viral DNA levels, Haque, E., Bhandari, B. R., Gidley, M. J., Deeth, modification, Biomacromolecules 16, 2049- Archives of Virology 160, 2169-2180. H. C., and Whittaker, A. K. 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78 - AIBN Annual Report 2015 Kubala, M., Norwood, S., Gomez, G., Jones, Licona-Cassani, C., Cruz-Morales, P., Luo, J., Qian, G., Liu, J., and Xu, Z. P. (2015) A., Johnston, W. A., Yap, A. S. K., Mureev, Manteca, A., Barona-Gomez, F., Nielsen, L. Anaerobic methanogenesis of fresh leachate S., and Alexandrov, K. (2015) Mammalian K., and Marcellin, E. (2015) Systems biology from municipal solid waste: a brief review on farnesyltransferase α subunit regulates approaches to understand natural products current progress, Renewable and Sustainable vacuolar protein sorting-associated protein 4A biosynthesis, Frontiers in Bioengineering and Energy Reviews 49, 21-28. (Vps4A) – dependent intracellular trafficking Biotechnology 3, 199.191-199.198. through recycling endosomes, Biochemical and Lyu, M., Yun, J.-H., Ahmed, R., Elkington, D., Biophysical Research Communications 468, Lightowlers, M. W., Assana, E., Jayashi, C. M., Wang, Q., Zhang, M., Wang, H., Dastoor, P., 580-586. Gauci, C. 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AIBN Annual Report 2015 - 79 Medina-Torres, C. E., Underwood, C., Pollitt, Mohoney, S. A., Rufford, T., Rudolph, V., Liu, K.-Y., tuberculosis—toward rapid screening for C. C., Castro-Olivera, E. M., Hodson, M. P., Rodrigues, S., and Steel, K. (2015) Creation of active case finding, ACS Sensors, doi:10.1021/ Richardson, D. W., and van Eps, A. W. (2015) micro channels in Bowen Basin coals using acssensors.1025b00171. Microdialysis measurements of equine lamellar UV laser and reactive ion etching, International perfusion and energy metabolism in response Journal of Coal Geology 144-145, 48-57. Ng, B. Y. C., Xiao, W., West, N. P., Wee, E. J. H., to physical and pharmacological manipulations Wang, Y., and Trau, M. (2015) Rapid, Single-Cell of blood flow, Equine Veterinary Journal, Monteiro, M. J. (2015) Fitting molecular weight Electrochemical Detection of Mycobacterium doi:10.1111/evj.12511. distributions using a log-normal distribution tuberculosis Using Colloidal Gold Nanoparticles, model, European Polymer Journal 65, 197-201. Analytical Chemistry 87, 10613-10618. Medina-Torres, C. E., Underwood, C., Pollitt, C. C., Castro-Olivera, E. M., Hodson, M. P., Moslehyani, A., Mobaraki, M., Ismail, A. F., Nguyen, T. T., Parat, M.-O., Hodson, M. P., Richardson, D. W., and Van Eps, A. W. (2015) Matsuura, T., Hashemifard, S. A., Othman, M. Pan, J., Shaw, P. N., and Hewavitharana, A. K. Microdialysis measurements of lamellar H. D., Mayahi, A., Rezaei Dashtarzhandi, M., (2015) Chemical characterization and in vitro perfusion and energy metabolism during the Soheilmoghaddam, M., and Shamsaei, E. (2015) cytotoxicity on squamous cell carcinoma cells development of laminitis in the oligofructose Effect of HNTs modification in nanocomposite of Carica papaya leaf extracts, Toxins 8, 7.1- model, Equine Veterinary Journal 48, 246-252. membrane enhancement for bacterial removal 7.11. by cross-flow ultrafiltration system, Reactive Medina-Torres, C. E., van Eps, A. W., Nielsen, and Functional Polymers 95, 80-87. Niu, X., Zhao, H., Lan, M., and Zhou, L. (2015) L. K., and Hodson, M. P. (2015) A liquid Platinum nanoparticles encapsulated in carbon chromatography-tandem mass spectrometry- Mukundan, S., Konarova, M., Atanda, L., microspheres: toward electro-catalyzing based investigation of the lamellar interstitial Ma, Q., and Beltramini, J. 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AIBN Annual Report 2015 - 81 Methylome sequencing in triple negative breast Tekewe, A., Connors, N. K., Sainsbury, F., Wang, B., Bai, Y., Xing, Z., Hulicova-Jurcakova, cancer reveals distinct methylation clusters Wibowo, N., Lua, L. H. L., and Middelberg, A. P. D., and Wang, L. (2015) Enhanced performance with prognostic value, Nature Communications J. (2015) A rapid and simple screening method of a pillared TiO2 nanohybrid as an anode 6, 1-11. to identify conditions for enhanced stability material for fast and reversible lithium storage, of modular vaccine candidates, Biochemical ChemNanoMat 1, 96-101. Stone, A., Zotenko, E., Locke, W. J., Korbie, D., Engineering Journal 100, 50-58. Millar, E. K. A., Pidsley, R., Stirzaker, C., Graham, Wang, B., Liu, A., Abdulla, W. A., Wang, D., and P., Trau, M., Musgrove, E. A., Nicholson, R. I., Gee, Thakar, N. Y., Ovchinnikov, D. A., Hastie, M. L., Zhao, X. S. (2015) Desired crystal oriented J. M. W., and Clark, S. J. 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82 - AIBN Annual Report 2015 M@B9/M@B9- (M = Ir, Rh, Ru) clusters: a Wibowo, N., Wu, Y., Fan, Y., Meers, J., Lua, L. different amylose contents plasticised theoretical study, Journal of Physical Chemistry H. L., and Middelberg, A. P. J. (2015) Non- by 1-ethyl-3-methylimidazolium acetate, A 119, 796-805. chromatographic preparation of a bacterially Carbohydrate Polymers 122, 160-168. produced single-shot modular virus-like particle Wang, Y., Li, Y., Xu, T., Shi, Z., and Wu, Q. capsomere vaccine for avian influenza, Vaccine Xie, M. X., Jiang, L., Xu, Z. P., and Chen, D. Y. (2015) Experimental evidence for growth 33, 5960-5965. (2015) Monofunctional polymer nanoparticles advantage and metabolic shift stimulated by prepared through intramolecularly cross-linking photophosphorylation of proteorhodopsin Willenbacher, J., Altintas, O., Trouillet, V., Knofel, the polymer chains sparsely grafted on the expressed in Escherichia coli at anaerobic N., Monteiro, M. J., Roesky, P. 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(2015) Metabolic Engineering 29, 124-134. Xu, C., Yu, M., Noonan, O., Zhang, J., Song, Highly sensitive DNA methylation analysis at H., Zhang, H., Lei, C., Niu, Y., Huang, X., CpG resolution by surface-enhanced Raman Williams, T. C., Espinosa, M. I., Nielsen, L. K., and Yang, Y., and Yu, C. (2015) Core-cone scattering via ligase chain reaction, Chemical Vickers, C. E. (2015) Dynamic regulation of gene structured monodispersed mesoporous silica Communications 51, 10953-10956. expression using sucrose responsive promoters nanoparticles with ultra-large cavity for protein and RNA interference in Saccharomyces delivery, Small 11, 5949-5955. Wee, E. J. H., Lau, H. Y., Botella, J., and Trau, cerevisiae, Microbial Cell Factories 14, 1-10. M. (2015) Re-purposing bridging flocculation Xu, Y., Zhang, J., Liang, Y., Zhou, J., Zhao, for on-site, rapid, qualitative DNA detection Wong, N. C., Pope, B. J., Candiloro, I., Korbie, D., J., Ruan, X., Xu, Z. P., and Qian, G. 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AIBN Annual Report 2015 - 83 hydroxide/polypropylene nanocomposites, Zainal Abidin, N. I., Da Forno, A., Bestetti, Zhong, L., Chokkalingam, A., Cha, W. S., Lakhi, Journal of Materials Chemistry A 3, 22730- M., Martin, D., Beer, A., and Atrens, A. (2015) K. S., Su, X., Lawrence, G., and Vinu, A. (2015) 22738. Evaluation of coatings for Mg alloys for Pd nanoparticles embedded in mesoporous biomedical applications, Advanced Engineering carbon: A highly efficient catalyst for Suzuki- Yang, Q., Wu, J., Xu, Z. P., and Chen, D. (2015) Materials 17, 58-67. Miyaura reaction, Catalysis Today 243, 195-198. A new design of ionic complexation and its application for efficient protection of proteins, Zhang, B., Chen, L., Xie, F., Li, X., Truss, R. W., Zhou, C., Zheng, K., Lu, Z., Zhang, Z., Liao, Z., Polymer Chemistry 6, 1688-1692. Halley, P. J., Shamshina, J. L., Rogers, R. D., and Chen, P., Lu, W., and Zou, J. (2015) Quality McNally, T. 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84 - AIBN Annual Report 2015 T hank-you

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