Early Patterning and Specification of Cardiac Progenitors in Gastrulating
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The Evolving Cardiac Lymphatic Vasculature in Development, Repair and Regeneration
REVIEWS The evolving cardiac lymphatic vasculature in development, repair and regeneration Konstantinos Klaourakis 1,2, Joaquim M. Vieira 1,2,3 ✉ and Paul R. Riley 1,2,3 ✉ Abstract | The lymphatic vasculature has an essential role in maintaining normal fluid balance in tissues and modulating the inflammatory response to injury or pathogens. Disruption of normal development or function of lymphatic vessels can have severe consequences. In the heart, reduced lymphatic function can lead to myocardial oedema and persistent inflammation. Macrophages, which are phagocytic cells of the innate immune system, contribute to cardiac development and to fibrotic repair and regeneration of cardiac tissue after myocardial infarction. In this Review, we discuss the cardiac lymphatic vasculature with a focus on developments over the past 5 years arising from the study of mammalian and zebrafish model organisms. In addition, we examine the interplay between the cardiac lymphatics and macrophages during fibrotic repair and regeneration after myocardial infarction. Finally, we discuss the therapeutic potential of targeting the cardiac lymphatic network to regulate immune cell content and alleviate inflammation in patients with ischaemic heart disease. The circulatory system of vertebrates is composed of two after MI. In this Review, we summarize the current complementary vasculatures, the blood and lymphatic knowledge on the development, structure and function vascular systems1. The blood vasculature is a closed sys- of the cardiac lymphatic vasculature, with an emphasis tem responsible for transporting gases, fluids, nutrients, on breakthroughs over the past 5 years in the study of metabolites and cells to the tissues2. This extravasation of cardiac lymphatic heterogeneity in mice and zebrafish. -
1 Early Patterning and Specification of Cardiac Progenitors In
Early Patterning and Specification of Cardiac Progenitors in Gastrulating Mesoderm W. Patrick Devine1,2,3,4, Joshua D. Wythe1,2, Matthew George1,2,5 , Kazuko Koshiba- Takeuchi1,2, Benoit G. Bruneau1,2,4,5 1. Gladstone Institute of Cardiovascular Disease, San Francisco, CA, 94158 USA 2. Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, CA 94158, USA 3. Department of Pathology, University of California, San Francisco, CA 94143 USA 4. Cardiovascular Research Institute, University of California, San Francisco, CA 94158 USA 5. Developmental and Stem Cell Biology Program, University of San Francisco, CA 94143, USA 6. Department of Pediatrics, University of California, San Francisco, CA 94143 USA Competing interests statement: The authors declare no competing interests. 1 Abstract Mammalian heart development requires precise allocation of cardiac progenitors. The existence of a multipotent progenitor for all anatomic and cellular components of the heart has been predicted but its identity and contribution to the two cardiac progenitor "fields" has remained undefined. Here we show, using clonal genetic fate mapping, that Mesp1+ cells in gastrulating mesoderm are rapidly specified into committed cardiac precursors fated for distinct anatomic regions of the heart. We identify Smarcd3 as a marker of early specified cardiac precursors and identify within these precursors a compartment boundary at the future junction of the left and right ventricles that arises prior to morphogenesis. Our studies define the timing and hierarchy of cardiac progenitor specification and demonstrate that the cellular and anatomical fate of mesoderm-derived cardiac cells is specified very early. These findings will be important to understand the basis of congenital heart defects and to derive cardiac regeneration strategies. -
Supplemental Table 1. Complete Gene Lists and GO Terms from Figure 3C
Supplemental Table 1. Complete gene lists and GO terms from Figure 3C. Path 1 Genes: RP11-34P13.15, RP4-758J18.10, VWA1, CHD5, AZIN2, FOXO6, RP11-403I13.8, ARHGAP30, RGS4, LRRN2, RASSF5, SERTAD4, GJC2, RHOU, REEP1, FOXI3, SH3RF3, COL4A4, ZDHHC23, FGFR3, PPP2R2C, CTD-2031P19.4, RNF182, GRM4, PRR15, DGKI, CHMP4C, CALB1, SPAG1, KLF4, ENG, RET, GDF10, ADAMTS14, SPOCK2, MBL1P, ADAM8, LRP4-AS1, CARNS1, DGAT2, CRYAB, AP000783.1, OPCML, PLEKHG6, GDF3, EMP1, RASSF9, FAM101A, STON2, GREM1, ACTC1, CORO2B, FURIN, WFIKKN1, BAIAP3, TMC5, HS3ST4, ZFHX3, NLRP1, RASD1, CACNG4, EMILIN2, L3MBTL4, KLHL14, HMSD, RP11-849I19.1, SALL3, GADD45B, KANK3, CTC- 526N19.1, ZNF888, MMP9, BMP7, PIK3IP1, MCHR1, SYTL5, CAMK2N1, PINK1, ID3, PTPRU, MANEAL, MCOLN3, LRRC8C, NTNG1, KCNC4, RP11, 430C7.5, C1orf95, ID2-AS1, ID2, GDF7, KCNG3, RGPD8, PSD4, CCDC74B, BMPR2, KAT2B, LINC00693, ZNF654, FILIP1L, SH3TC1, CPEB2, NPFFR2, TRPC3, RP11-752L20.3, FAM198B, TLL1, CDH9, PDZD2, CHSY3, GALNT10, FOXQ1, ATXN1, ID4, COL11A2, CNR1, GTF2IP4, FZD1, PAX5, RP11-35N6.1, UNC5B, NKX1-2, FAM196A, EBF3, PRRG4, LRP4, SYT7, PLBD1, GRASP, ALX1, HIP1R, LPAR6, SLITRK6, C16orf89, RP11-491F9.1, MMP2, B3GNT9, NXPH3, TNRC6C-AS1, LDLRAD4, NOL4, SMAD7, HCN2, PDE4A, KANK2, SAMD1, EXOC3L2, IL11, EMILIN3, KCNB1, DOK5, EEF1A2, A4GALT, ADGRG2, ELF4, ABCD1 Term Count % PValue Genes regulation of pathway-restricted GDF3, SMAD7, GDF7, BMPR2, GDF10, GREM1, BMP7, LDLRAD4, SMAD protein phosphorylation 9 6.34 1.31E-08 ENG pathway-restricted SMAD protein GDF3, SMAD7, GDF7, BMPR2, GDF10, GREM1, BMP7, LDLRAD4, phosphorylation -
Genome-Wide Transcriptome and Binding Sites Analyses Identify
www.nature.com/scientificreports OPEN Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions Received:06October2015 Accepted:14July2016 for Enhancing Cardiomyogenesis Published:09August2016 Efficiency of hESC Cultures Hock Chuan Yeo1,2, Sherwin Ting1, Romulo Martin Brena3, Geoffrey Koh1, Allen Chen1, Siew Qi Toh2, Yu Ming Lim1, Steve Kah Weng Oh1 & Dong-Yup Lee1,2,4 The differentiation efficiency of human embryonic stem cells (hESCs) into heart muscle cells (cardiomyocytes) is highly sensitive to culture conditions. To elucidate the regulatory mechanisms involved, we investigated hESCs grown on three distinct culture platforms: feeder-free Matrigel, mouse embryonic fibroblast feeders, and Matrigel replated on feeders. At the outset, we profiled and quantified their differentiation efficiency, transcriptome, transcription factor binding sites and DNA- methylation. Subsequent genome-wide analyses allowed us to reconstruct the relevant interactome, thereby forming the regulatory basis for implicating the contrasting differentiation efficiency of the culture conditions. We hypothesized that the parental expressions of FOXC1, FOXD1 and FOXQ1 transcription factors (TFs) are correlative with eventual cardiomyogenic outcome. Through WNT induction of the FOX TFs, we observed the co-activation of WNT3 and EOMES which are potent inducers of mesoderm differentiation. The result strengthened our hypothesis on the regulatory role of the FOX TFs in enhancing mesoderm differentiation capacity of hESCs. Importantly, the final proportions of cells expressing cardiac markers were directly correlated to the strength of FOX inductions within 72 hours after initiation of differentiation across different cell lines and protocols. Thus, we affirmed the relationship between early FOX TF expressions and cardiomyogenesis efficiency. -
Development of Right Ventricle
DEVELOPMENT OF THE HEART II. David Lendvai M.D., Ph.D. Mark Kozsurek, M.D., Ph.D. • Septation of the common atrioventricular (AV) orifice. • Formation of the interatrial septum. • Formation of the muscular interventricular septum. • Appearance of the membranous interventricular septum and the spiral aorticopulmonary septum. right left septum primum septum primum septum primum septum primum septum primum septum primum foramen primum foramen primum septum primum septum primum foramen primum foramen primum septum primum septum primum foramen secundum foramen secundum foramen primum foramen primum septum primum foramen secundum septum primum foramen secundum foramen primum foramen primum septum primum septum primum foramen secundum foramen secundum septum secundum septum secundum foramen secundum foramen ovale foramen ovale septum primum septum primum septum secundum septum secundum foramen secundum foramen ovale foramen ovale septum primum septum primum septum secundum septum secundum foramen secundum septum primum foramen ovale foramen ovale septum primum SUMMARY • The septation of the common atrium starts with the appearance of the crescent-shaped septum primum. The opening of this septum, the foramen primum, becomes progressively smaller. • Before the foramen primum completly closes, postero-superiorly several small openings appear on the septum primum. These perforations coalesce later and form the foramen secundum. • On the right side of the septum primum a new septum, the septum secundum, starts to grow. The orifice of the septum secundum is the foramen ovale. • Finally two crescent-like, incomplete, partially overlapping septa exist with one hole on each. Septum secundum is more rigid and the septum primum on its left side acts as a valve letting the blood flow exclusively from the right to the left. -
Abnormalities of Placental Development and Function Are
bioRxiv preprint doi: https://doi.org/10.1101/388074; this version posted August 27, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abnormalities of placental development and function are associated with the different fetal growth patterns of hypoplastic left heart syndrome and transposition of the great arteries. Weston Troja1, Kathryn J. Owens1, Jennifer Courtney1, Andrea C. Hinton3, Robert B. Hinton3, James F. Cnota2 and Helen N. Jones1* 1. Center for Fetal and Placental Therapy, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Ave, Cincinnati, Ohio 2. Heart Institute, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Ave, Cincinnati, Ohio 3. TriHealth Maternal Fetal Medicine, 375 Dixsmyth Ave, Cincinnati Ohio *Corresponding Author: Helen N. Jones [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/388074; this version posted August 27, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Background: Birthweight is a critical predictor of congenital heart disease (CHD) surgical outcomes. Hypoplastic left heart syndrome (HLHS) is cyanotic CHD with known fetal growth restriction and placental abnormalities. Transposition of the great arteries (TGA) is cyanotic CHD with normal fetal growth. Comparison of the placenta in these diagnoses may provide insights on the fetal growth abnormality of CHD. Methods: Clinical data and placental histology from placentas associated with Transposition of the Great Arteries (TGA) were analyzed for gross pathology, morphology, maturity and vascularity and compared to both control and previously analyzed HLHS placentas [1]. -
Conditional Mutation of Hand1 in the Mouse Placenta Disrupts Placental Vascular Development Resulting in Fetal Loss in Both Early and Late Pregnancy
International Journal of Molecular Sciences Article Conditional Mutation of Hand1 in the Mouse Placenta Disrupts Placental Vascular Development Resulting in Fetal Loss in Both Early and Late Pregnancy Jennifer A. Courtney 1, Rebecca L. Wilson 2,3, James Cnota 4 and Helen N. Jones 2,3,* 1 Center for Fetal and Placental Therapy, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA; [email protected] 2 Center for Research in Perinatal Outcomes, College of Medicine, University of Florida, Gainesville, FL 32603, USA; rebecca.wilson@ufl.edu 3 Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL 32603, USA 4 Heart Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA; [email protected] * Correspondence: jonesh@ufl.edu; Tel.: +1-352-846-1503 Abstract: Congenital heart defects (CHD) affect approximately 1% of all live births, and often require complex surgeries at birth. We have previously demonstrated abnormal placental vascularization in human placentas from fetuses diagnosed with CHD. Hand1 has roles in both heart and placen- tal development and is implicated in CHD development. We utilized two conditionally activated Hand1A126fs/+ murine mutant models to investigate the importance of cell-specific Hand1 on placental development in early (Nkx2-5Cre) and late (Cdh5Cre) pregnancy. Embryonic lethality occurred in Citation: Courtney, J.A.; Wilson, R.L.; Nkx2-5Cre/Hand1A126fs/+ embryos with marked fetal demise occurring after E10.5 due to a failure Cnota, J.; Jones, H.N. Conditional in placental labyrinth formation and therefore the inability to switch to hemotrophic nutrition or Mutation of Hand1 in the Mouse Placenta Disrupts Placental Vascular maintain sufficient oxygen transfer to the fetus. -
Functional Morphology of the Cardiac Jelly in the Tubular Heart of Vertebrate Embryos
Review Functional Morphology of the Cardiac Jelly in the Tubular Heart of Vertebrate Embryos Jörg Männer 1,*,† and Talat Mesud Yelbuz 2,† 1 Group Cardio‐Embryology, Institute of Anatomy and Embryology UMG, Georg‐August‐University Goettingen, D‐37075 Goettingen, Germany; [email protected] 2 Department of Cardiac Sciences, King Abdulaziz Cardiac Center, Section of Pediatric Cardiology, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh 11426, Saudi Arabia; [email protected] * Correspondence: [email protected]; Tel.: +49‐551‐39‐7032 † This work is dedicated to the memory of our academic mentors Gerd Steding (1936–2011) and Armin Wessel (1946–2011). Received: 29 January 2019; Accepted: 21 February 2019; Published: 27 February 2019 Abstract: The early embryonic heart is a multi‐layered tube consisting of (1) an outer myocardial tube; (2) an inner endocardial tube; and (3) an extracellular matrix layer interposed between the myocardium and endocardium, called “cardiac jelly” (CJ). During the past decades, research on CJ has mainly focused on its molecular and cellular biological aspects. This review focuses on the morphological and biomechanical aspects of CJ. Special attention is given to (1) the spatial distribution and fiber architecture of CJ; (2) the morphological dynamics of CJ during the cardiac cycle; and (3) the removal/remodeling of CJ during advanced heart looping stages, which leads to the formation of ventricular trabeculations and endocardial cushions. CJ acts as a hydraulic skeleton, displaying striking structural and functional similarities with the mesoglea of jellyfish. CJ not only represents a filler substance, facilitating end‐systolic occlusion of the embryonic heart lumen. -
Conserved Signaling Mechanisms in Drosophila Heart Development
DEVELOPMENTAL DYNAMICS 246:641–656, 2017 DOI: 10.1002/DVDY.24530 REVIEWS Conserved Signaling Mechanisms in Drosophila Heart Development a Shaad M. Ahmad 1,2* 1Department of Biology, Indiana State University, Terre Haute, Indiana 2The Center for Genomic Advocacy, Indiana State University, Terre Haute, Indiana Abstract: Signal transduction through multiple distinct pathways regulates and orchestrates the numerous biological pro- cesses comprising heart development. This review outlines the roles of the FGFR, EGFR, Wnt, BMP, Notch, Hedgehog, Slit/ Robo, and other signaling pathways during four sequential phases of Drosophila cardiogenesis—mesoderm migration, cardiac mesoderm establishment, differentiation of the cardiac mesoderm into distinct cardiac cell types, and morphogenesis of the heart and its lumen based on the proper positioning and cell shape changes of these differentiated cardiac cells—and illus- trates how these same cardiogenic roles are conserved in vertebrates. Mechanisms bringing about the regulation and combi- natorial integration of these diverse signaling pathways in Drosophila are also described. This synopsis of our present state of knowledge of conserved signaling pathways in Drosophila cardiogenesis and the means by which it was acquired should facili- tate our understanding of and investigations into related processes in vertebrates. Developmental Dynamics 246:641–656, 2017. VC 2017 Wiley Periodicals, Inc. Key words: heart development; Drosophila cardiogenesis; cardiac mesoderm specification; cardiac morphogenesis; -
Grimme, Acadia.Pdf
MECHANISM OF ACTION OF HISTONE DEACETYLASE INHIBITORS ON SURVIVAL MOTOR NEURON 2 PROMOTER by Acadia L. Grimme A thesis submitted to the Faculty of the University of Delaware in partial fulfillment of the requirements for the degree of Bachelors of Science in Biological Sciences with Distinction Spring 2018 © 2018 Acadia Grimme All Rights Reserved MECHANISM OF ACTION OF HISTONE DEACETYLASE INHIBITORS ON SURVIVAL MOTOR NEURON 2 PROMOTER by Acadia L. Grimme Approved: __________________________________________________________ Matthew E. R. Butchbach, Ph.D. Professor in charge of thesis on behalf of the Advisory Committee Approved: __________________________________________________________ Deni S. Galileo, Ph.D. Professor in charge of thesis on behalf of the Advisory Committee Approved: __________________________________________________________ Carlton R. Cooper, Ph.D. Committee member from the Department of Biological Sciences Approved: __________________________________________________________ Gary H. Laverty, Ph.D. Committee member from the Board of Senior Thesis Readers Approved: __________________________________________________________ Michael Chajes, Ph.D. Chair of the University Committee on Student and Faculty Honors ACKNOWLEDGMENTS I would like to acknowledge my thesis director Dr. Butchbach for his wonderful guidance and patience as I worked through my project. He has been an excellent research mentor over the last two years and I am forever thankful that he welcomed me into his lab. His dedication to his work inspires me as an aspiring research scientist. His lessons will carry on with me as I pursue future research in graduate school and beyond. I would like to thank both current and former members of the Motor Neuron Disease Laboratory: Sambee Kanda, Kyle Hinkle, and Andrew Connell. Sambee and Andrew patiently taught me many of the techniques I utilized in my project, and without them it would not be what it is today. -
Cardiogenesis in the Bovine to 35 Somites
CARDIOGENESIS IN THE BOVINE TO 35 SOMITES by PATRICIA ANN NODEN A. A., Chanute Junior College, 1962 B. S., Kansas State University, 1964 A MASTER'S THESIS submitted in partial fulfillment of the requirements for the degree MASTER OF SCIENCE Department of Zoology KANSAS STATE UNIVERSITY Manhattan, Kansas 1966 Approved by: ii N7G> TABLE OF CONTENTS Oocu mtA t INTRODUCTION 1 METHODS AND MATERIALS 6 OBSERVATIONS 9 Presomite Stage 9 Six Somite Stage 9 Nine Somite Stage 10 13 Somite Stage 18 19 Somite Stage 18 22 Somite Stage 19 24-25 Somite Stage 20 28-30 Somite Stage ....... 21 33-35 Somite Stage 24 DISCUSSION 27 CONCLUSION S3 ACKNOWLEDGMENTS 35 BIBLIOGRAPHY . 36 . INTRODUCTION Mammalian cardiogenesis is a vast field which so far has not been thoroughly explored. There are few species in which the complete development of the heart has been studied and many in which partial formation has been observed The formation of the heart to the functional stage in the dog (14 somites) was studied by Bonnet (1901) and Duffey (1953). Duffey's thesis was used exclusively for comparison in this paper. The rat has been studied in some detail, from presomite to birth and after, by Burlingame and Long (1939) and early stages by Ravn (1895) and Goss (1942, 1952). The tubular phase of cardiogenesis in the rabbit of 10-13 somites was described by Girgis (1930, 1933). Dwinnel (1939) worked with early somite stages. Development of the aortic arches was described by Bremer (1912). Hensen (1875), Bom (1888, 1889), Strahl and Carius (1889) and Rouviere (1904) have described early cardiac formation. -
Functional Morphology of the Cardiac Jelly in the Tubular
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 30 January 2019 doi:10.20944/preprints201901.0312.v1 Peer-reviewed version available at J. Cardiovasc. Dev. Dis. 2019, 6, 12; doi:10.3390/jcdd6010012 1 Review 2 Functional Morphology of the Cardiac Jelly in the 3 Tubular Heart of Vertebrate Embryos 4 Jörg Männer 1,* and Talat Mesud Yelbuz 2 5 1 Group Cardio-Embryology, Institute of Anatomy and Embryology UMG, Georg-August-University 6 Goettingen, D-37075 Goettingen, Germany; [email protected] 7 2 Department of Cardiac Sciences, King Abdulaziz Cardiac Center, Section of Pediatric Cardiology, King 8 Abdulaziz Medical City, Ministry of National Guard Health Affairs; Riyadh, Kingdom of Saudi Arabia; 9 [email protected] 10 * Correspondence: [email protected]; Tel.: +49-551-39-7032 11 12 Abstract: The early embryonic heart is a multi-layered tube consisting of (1) an 13 outer myocardial tube; (2) an inner endocardial tube; and (3) an extracellular 14 matrix layer interposed between myocardium and endocardium, called “cardiac 15 jelly” (CJ). During the past decades, research on CJ has mainly focused on its 16 molecular and cell biological aspects. This review focuses on the morphological 17 and biomechanical aspects of CJ. Special attention is given to (1) the spatial 18 distribution and fiber architecture of CJ; (2) the morphological dynamics of CJ 19 during the cardiac cycle; and (3) the removal/remodeling of CJ during advanced 20 heart looping stages, which leads to the formation of ventricular trabeculations 21 and endocardial cushions. CJ acts as a hydraulic skeleton displaying striking 22 structural and functional similarities with the mesoglea of jellyfish.