Morvan Et Al. Timetree of Aselloidea Reveals Species Diversification

Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

Appendix 2 COI dataset. CO : iso country codes, LA : latitude in decimal degrees, LO : longitude in decimal degrees, HA : haplotype number, TH : evolutionary unit number as delimited with the threshold method, GM : evolutionary unit number as delimited with the GMYC method, AN : accession number.

SPECIES INDIVIDUAL LOCALITY CO LA LO HA TH GM AN Asellus (Asellus) aquaticus (Linnaeus, 1758) Alborg DK 57.04722 9.91944 H_478 T_176 G_035 DQ144816 ∗ Feres GR 40.85083 26.17750 H_479 T_174 G_143 DQ144885 ∗ Karlobag HR 44.52667 15.18139 H_484 T_175 G_146 DQ144788 ∗ Kharkiv UA 50.03917 36.16167 H_483 T_172 G_142 DQ144824 ∗ Lezignan FR 43.26694 2.71944 H_477 T_176 G_035 AY531784 ∗ Olsztyn PL 53.81139 20.43667 H_480 T_176 G_035 DQ144821 ∗ Santorin island GR 36.45278 25.50556 H_482 T_174 G_144 DQ144828 † Sheﬃeld GB 53.49444 -1.24778 H_476 T_176 G_035 DQ144886 ∗ Trieste IT 45.67258 13.82487 H_481 T_173 G_145 AY531762 ∗ Wageningen NL 51.96778 5.66278 H_485 T_176 G_035 DQ144844 † Asellus (Asellus) hilgendorﬁi Bovallius, 1886 Toyama JP 36.69583 137.21361 H_486 T_177 G_140 AY531829 † Betamorpha fusiformis (Barnard, 1920) Weddell Sea -75.04944 -47.26278 H_496 T_020 G_121 EF682291 ‡ Bragasellus comasi Henry & Magniez, 1976 COBALLE1_200906_1 Caso ES 43.18619 -5.38696 H_364 T_155 G_039 JQ921527 COBALLE2_200906_1 Caso ES 43.18470 -5.38804 H_365 T_155 G_039 JQ921528 COBALLE2_200906_2 Caso ES 43.18470 -5.38804 H_366 T_158 G_155 JQ921529 FRIES_200906_1ID Cangas de Onis ES 43.42265 -5.08772 H_369 T_157 G_040 JQ921532 FRIES_200906_2 Cangas de Onis ES 43.42265 -5.08772 H_370 T_157 G_040 JQ921533 FRIES_200906_3 Cangas de Onis ES 43.42265 -5.08772 H_369 T_157 G_040 JQ921534 LAFOZ_200906_1 Caso ES 43.14939 -5.27504 H_371 T_155 G_039 JQ921535 PENDONES_200906_1ID Caso ES 43.13238 -5.24747 H_367 T_154 G_153 JQ921530 PENDONES_200906_2ID Caso ES 43.13238 -5.24747 H_368 T_155 G_039 JQ921531 Bragasellus cortesi Afonso, 1989 POUCA_201010_1ID Vila Pouca de Aguiar PT 41.49062 -7.64903 H_372 T_169 G_045 JQ921536 POUCA_201010_2 Vila Pouca de Aguiar PT 41.49062 -7.64903 H_372 T_169 G_045 JQ921537 POUCA_201010_3 Vila Pouca de Aguiar PT 41.49062 -7.64903 H_373 T_169 G_045 JQ921538 Bragasellus escolai Henry & Magniez, 1978 YUSOREIN_200906_1ID Portilla de la Reina ES 43.02150 -4.86578 H_374 T_160 G_041 JQ921539 YUSOREIN_200906_4ID Portilla de la Reina ES 43.02150 -4.86578 H_375 T_160 G_041 JQ921540 Bragasellus frontellum (Braga, 1964) ABOIM_201010_1ID Aboim das Chocas PT 41.91711 -8.44715 H_376 T_170 G_156 JQ921541 ABOIM_201010_2 Aboim das Chocas PT 41.91711 -8.44715 H_376 T_170 G_156 JQ921542 ABOIM_201010_2ex Aboim das Chocas PT 41.91711 -8.44715 H_376 T_170 G_156 JQ921543 Bragasellus lagari Henry & Magniez, 1973 ALBENTOS_200910_1ID Albentosa ES 40.12242 -0.76379 H_377 T_026 G_137 JQ921544 ARGONGE_200910_1ex Teresa de Cofrentes ES 39.10269 -1.04100 H_382 T_025 G_029 JQ921525 ARGONGE_200910_2ex Teresa de Cofrentes ES 39.10269 -1.04100 H_383 T_025 G_029 JQ921526 CABRIEL_200910_1ID Cofrentes ES 39.24954 -1.09382 H_378 T_023 G_030 JQ921552 CABRIEL_200910_2 Cofrentes ES 39.24954 -1.09382 H_379 T_023 G_030 JQ921553 CASCAN2_200910_1ID Cascante del Rio ES 40.20431 -1.12963 H_381 T_022 G_031 JQ921545 CASCAN2_200910_2 Cascante del Rio ES 40.20431 -1.12963 H_381 T_022 G_031 JQ921546 CAUTAB_200910_2ID Jarafuel ES 39.14380 -1.04686 H_381 T_023 G_030 JQ921549 CAUTAB_200910_3ID Jarafuel ES 39.14380 -1.04686 H_380 T_023 G_030 JQ921550 CAUTAB_200910_5 Jarafuel ES 39.14380 -1.04686 H_380 T_023 G_030 JQ921551 MEREMIL_200910_1ID Gestalgar ES 39.59690 -0.84903 H_385 T_021 G_136 JQ921547 MEREMIL_200910_2 Gestalgar ES 39.59690 -0.84903 H_385 T_021 G_136 JQ921548 SUMIDORS_200910_1ID Vallada ES 38.88048 -0.68871 H_384 T_027 G_134 JQ921555 SUMIDORS_200910_2 Vallada ES 38.88048 -0.68871 H_362 T_027 G_134 JQ921556 TRILLO_200910_2 Trillo ES 40.69067 -2.58333 H_363 T_024 G_135 JQ921554 Bragasellus lagarioides Henry & Magniez, 1996 CREUS_200906_1 Santes Creus ES 41.34883 1.36169 H_386 T_162 G_037 JQ921557 MARFIL_200906_1 Valdenoceda ES 42.86092 -3.59298 H_387 T_156 G_043 JQ921558 MARFIL_200906_2 Valdenoceda ES 42.86092 -3.59298 H_388 T_156 G_043 JQ921559 MARFIL_200906_4ID Valdenoceda ES 42.86092 -3.59298 H_388 T_156 G_043 JQ921560 PERPETUA_200906_1ID Santa Perpetua de Gaya ES 41.46251 1.39087 H_389 T_162 G_037 JQ921561

1 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

PERPETUA_200906_2 Santa Perpetua de Gaya ES 41.46251 1.39087 H_389 T_162 G_037 JQ921562 RUDRON_201010_1ID Basconcillos del Tozo ES 42.70230 -3.91793 H_390 T_156 G_043 JQ921563 RUDRON_201010_2 Basconcillos del Tozo ES 42.70230 -3.91793 H_391 T_156 G_043 JQ921564 Bragasellus molinai Henry & Magniez, 1988 VALPORQU_201010_1ID Vegacervera ES 42.90665 -5.56286 H_392 T_163 G_038 JQ921565 VALPORQU_201010_2 Vegacervera ES 42.90665 -5.56286 H_393 T_163 G_038 JQ921566 VALPORQU_201010_3 Vegacervera ES 42.90665 -5.56286 H_394 T_163 G_038 JQ921567 Bragasellus peltatus (Braga, 1944) MEZIO_201010_1ID Lousada PT 41.28193 -8.30839 H_395 T_171 G_046 JQ921568 MEZIO_201010_2 Lousada PT 41.28193 -8.30839 H_396 T_171 G_046 JQ921569 SOUTO_201010_1ID Croca PT 41.22073 -8.24852 H_395 T_171 G_046 JQ921570 Bragasellus stocki Henry & Magniez, 1988 CONCHA_201010_2cyto Cudillero ES 43.55978 -6.19003 H_361 T_161 G_042 JQ921571 CONCHA_201010_3 Cudillero ES 43.55978 -6.19003 H_399 T_161 G_042 JQ921524 Bragasellus rouchi Henry & Magniez, 1988 DEVAPANE_200906_1ID Panes ES 43.31075 -4.60950 H_397 T_159 G_154 JQ921573 Caecidotea kenki (Bowman, 1967) PIMMIT1_200902_1 Fairfax US 38.92917 -77.11806 H_400 T_033 G_036 JQ921574 PIMMIT1_200902_2ID Fairfax US 38.92917 -77.11806 H_401 T_033 G_036 JQ921575 Chthonasellus bodoni Argano & Messana, 1991 CHIUPESI_200908_1ID Chiusa di Pesio IT 44.21600 7.65983 H_402 T_074 G_091 JQ921576 CHIUPESI_200908_2ID Chiusa di Pesio IT 44.21600 7.65983 H_403 T_074 G_091 JQ921577 CHIUPESI_200908_3 Chiusa di Pesio IT 44.21600 7.65983 H_403 T_074 G_091 JQ921578 Gallasellus heilyi (Legrand, 1956) FONTROI_200812_1ID La Jarrie-Audouin FR 46.03528 -0.46417 H_409 T_179 G_034 JQ921587 FONTROI_200812_2 La Jarrie-Audouin FR 46.03528 -0.46417 H_409 T_179 G_034 JQ921588 FONTVEUV_200812_2ID Saint-Pierre-de-l’Ile FR 46.02028 -0.44917 H_411 T_179 G_034 JQ921590 FONTVEUV_200812_3 Saint-Pierre-de-l’Ile FR 46.02028 -0.44917 H_412 T_179 G_034 JQ921591 MERIGNY_200908_1ex Merigny FR 46.64083 0.93472 H_408 T_178 G_141 JQ921585 MERIGNY_200912_1ID Merigny FR 46.64083 0.93472 H_408 T_178 G_141 JQ921586 PUITD_200912_1ID Le Thou FR 46.08472 -0.92528 H_406 T_180 G_032 JQ921582 PUITD_200912_2 Le Thou FR 46.08472 -0.92528 H_407 T_180 G_032 JQ921583 PUITD_200912_3 Le Thou FR 46.08472 -0.92528 H_407 T_180 G_032 JQ921584 SOMPT_200812_1ID Sompt FR 46.15611 -0.10194 H_410 T_180 G_032 JQ921589 Lirceolus bisetus (Steeves, 1968) San Saba US 31.01778 -98.50514 H_487 T_034 G_150 AY566533 § Lirceolus cocytus Lewis, 2001 Acuna MX 29.40254 -101.33401 H_489 T_029 G_138 AY566528 § Jeff Davis US 30.75278 -103.59917 H_488 T_028 G_139 AY566544 § Lirceolus hardeni Lewis & Bowman, 1996 Kendall US 29.90917 -98.61806 H_493 T_036 G_152 AY566537 § Travis US 30.26750 -97.76639 H_492 T_035 G_151 AY566551 § Lirceolus pilus (Steeves, 1968) Bandera US 29.77278 -99.56722 H_491 T_031 G_148 AY566539 § Medina US 29.52389 -99.40500 H_490 T_032 G_147 AY566554 § Magniezia gardei Magniez, 1978 ERRACHID_201004_1ID Errachidia MA 31.95300 -4.45240 H_422 T_014 G_132 JQ921605 ERRACHID_201004_2 Errachidia MA 31.95300 -4.45240 H_422 T_014 G_132 JQ921606 Mexistenasellus coahuila Cole & Minckley, 1972 San Antonio US 29.42337 -98.48781 H_495 T_018 G_133 AY566478 § Proasellus aff. escolai Henry & Magniez, 1982 JESUS_200910_1ID Iznatoraf ES 38.13233 -2.96932 H_003 T_109 G_204 JQ921020 JESUS_200910_2 Iznatoraf ES 38.13233 -2.96932 H_003 T_109 G_204 JQ921021 JESUS_200910_3 Iznatoraf ES 38.13233 -2.96932 H_003 T_109 G_204 JQ921022 Proasellus aff. lagari Henry & Magniez, 1982 PERAL_200910_1ID Sorbas ES 37.07920 -2.10080 H_004 T_117 G_201 JQ921023 PERAL_200910_2 Sorbas ES 37.07920 -2.10080 H_004 T_117 G_201 JQ921024 PERAL_200910_3 Sorbas ES 37.07920 -2.10080 H_004 T_117 G_201 JQ921025 Proasellus albigensis (Magniez, 1965) FOUSTER_200804_1 Cambon FR 43.91028 2.22278 H_006 T_126 G_009 JQ921028 LAUZIE_200804_2ID Cambon FR 43.90972 2.23389 H_007 T_126 G_009 JQ921029 RAYSSAGU_197700_2ID Cambon FR 43.91028 2.22278 H_008 T_126 G_009 JQ921030 Proasellus aquaecalidae (Racovitza, 1922) GEAUXCH_200811_1m Eaux-Bonnes FR 42.93417 -0.44056 H_009 T_140 G_009 JQ921031 OUEILNEE_200811_1ID Rebenacq FR 43.15000 -0.40472 H_010 T_141 G_114 JQ921032

2 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

OUEILNEE_200811_2 Rebenacq FR 43.15000 -0.40472 H_011 T_141 G_114 JQ921033 OUEILNEE_200811_3 Rebenacq FR 43.15000 -0.40472 H_010 T_141 G_114 JQ921034 PEAUXCH_200811_1 Eaux-Bonnes FR 42.93861 -0.44028 H_009 T_140 G_191 JQ921035 PEAUXCH_200811_2 Eaux-Bonnes FR 42.93861 -0.44028 H_009 T_140 G_191 JQ921036 Proasellus aragonensis Henry & Magniez, 1992 BURGUI_200906_1ID Burgui ES 42.70362 -1.01646 H_012 T_147 G_113 JQ921037 BURGUI_200906_2 Burgui ES 42.70362 -1.01646 H_013 T_146 G_190 JQ921038 BURGUI_200906_3 Burgui ES 42.70362 -1.01646 H_014 T_147 G_113 JQ921039 Proasellus arnautovici (Remy, 1932) Ohrid MK 41.03730 20.71899 H_018 T_149 G_094 DQ305140 ¶ Ohrid MK 41.03730 20.71899 H_474 T_148 G_093 DQ305138 ¶ LIVADIST_200909_1ID Livadi MK 41.00746 20.75565 H_473 T_148 G_093 JQ921041 OSOJ1_200909_1ID Pod Osoj MK 40.95107 20.77643 H_016 T_149 G_094 JQ921040 TRPEJCA_200909_1ID Trpejca MK 40.96057 20.78289 H_015 T_148 G_093 JQ921042 VELIDAB_200909_1ID Veli Dab MK 41.00746 20.75565 H_017 T_148 G_093 JQ921043 Proasellus arthrodilus (Braga, 1945) LEGACAO_201010_1ID Legacao PT 40.03177 -8.46979 H_019 T_150 G_097 JQ921044 LEGACAO_201010_2 Legacao PT 40.03177 -8.46979 H_019 T_150 G_097 JQ921045 LEGACAO_201010_3 Legacao PT 40.03177 -8.46979 H_020 T_150 G_097 JQ921046 Proasellus assaforensis Afonso, 1988 ASSAFORA_201010_1ID Assofora PT 38.90872 -9.42192 H_021 T_128 G_200 JQ921047 ASSAFORA_201010_2 Assofora PT 38.90872 -9.42192 H_021 T_128 G_200 JQ921048 ASSAFORA_201010_3 Assofora PT 38.90872 -9.42192 H_021 T_128 G_200 JQ921049 Proasellus bardaunii Alouf, Henry & Magniez, 1982 QATTINE_201010_1ID Tarchich LB 33.87570 35.79730 H_022 T_049 G_167 JQ921050 QATTINE_201010_2 Tarchich LB 33.87570 35.79730 H_022 T_049 G_167 JQ921051 Proasellus bellesi Henry & Magniez, 1982 TURON_200910_1ID El Burgo ES 36.78994 -4.94127 H_023 T_115 G_019 JQ921052 TURON_200910_2 El Burgo ES 36.78994 -4.94127 H_024 T_115 G_019 JQ921053 TURON_201010_3cyto El Burgo ES 36.78994 -4.94127 H_024 T_115 G_019 JQ921054 Proasellus beroni Henry & Magniez, 1968 ASINAIA_200810_1ID Ponte Novu FR 42.49306 9.29472 H_029 T_097 G_105 JQ921060 BERNACCI_200810_3 Castifao FR 42.54583 9.13722 H_025 T_095 G_185 JQ921055 CASALUNA_200810_2 Lano FR 42.38750 9.25861 H_027 T_097 G_187 JQ921057 CASALUNA_200810_3 Lano FR 42.38750 9.25861 H_027 T_097 G_187 JQ921058 CASAMOZA_200810_1ID Lucciana FR 42.51861 9.44222 H_026 T_097 G_105 JQ921056 ORTAMIN_200810_1ex Corte FR 42.31861 9.15083 H_028 T_096 G_186 JQ921059 Proasellus beticus Henry & Magniez, 1992 SUMIDORS_200910_1ID Vallada ES 38.88048 -0.68871 H_030 T_107 G_198 JQ921061 SUMIDORS_200910_2 Vallada ES 38.88048 -0.68871 H_030 T_107 G_198 JQ921062 SUMIDORS_200910_3 Vallada ES 38.88048 -0.68871 H_030 T_107 G_198 JQ921063 Proasellus boui Henry & Magniez, 1969 SAUVEGAR_0_1ID Sauve FR 43.94056 3.95000 H_031 T_039 G_059 JQ921064 SAUVEGAR_0_2ex Sauve FR 43.94056 3.95000 H_032 T_039 G_059 JQ921065 SAUVEGAR_0_3ex Sauve FR 43.94056 3.95000 H_033 T_039 G_059 JQ921066 Proasellus cantabricus Henry & Magniez, 1968 RESCANO_200906_1ID Mirones ES 43.29376 -3.69926 H_034 T_094 G_102 JQ921067 RESCANO_200906_2 Mirones ES 43.29376 -3.69926 H_035 T_094 G_102 JQ921068 RESCANO_200906_3 Mirones ES 43.29376 -3.69926 H_035 T_094 G_102 JQ921069 Proasellus cavaticus (Leydig, 1871) ARBOUSSE_0_1ID Ceilhes-et-Rocozels FR 43.82250 3.14361 H_052 T_081 G_073 JQ921087 BELLEGAR_200902_1ID Jaun CH 46.61139 7.27583 H_039 T_081 G_075 JQ921073 BELLEGAR_200902_2 Jaun CH 46.61139 7.27583 H_040 T_081 G_075 JQ921074 BELLEGAR_200902_3 Jaun CH 46.61139 7.27583 H_041 T_081 G_075 JQ921075 BELMONT_200707_1ID Belmont FR 47.00945 5.59472 H_042 T_079 G_174 JQ921076 BORCASSO_200711_1ID Nevy-sur-Seille FR 46.73667 5.64222 H_043 T_078 G_173 JQ921077 BURKH3_200905_2 Burkheim DE 48.09513 7.59328 H_044 T_081 G_074 JQ921078 BURKH3_200905_3 Burkheim DE 48.09513 7.59328 H_045 T_081 G_074 JQ921079 CRAMANS_200808_4ID Challes-la-Montagne FR 46.13556 5.47611 H_053 T_081 G_073 JQ921088 CRUPET_200807_3ID Assesse BE 50.35449 4.96145 H_046 T_081 G_073 JQ921080 CRUPET_200807_4 Assesse BE 50.35449 4.96145 H_046 T_081 G_073 JQ921081 ELM_200904_1ID Clydach GB 51.80521 -3.13996 H_047 T_081 G_073 JQ921082 FRAITE_200711_1 Thoiria FR 46.52500 5.73389 H_038 T_080 G_172 JQ921072 ISIS_200901_1ex Aveze FR 43.98361 3.59806 H_048 T_081 G_073 JQ921083

3 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

JALLIFIE_0_10 Choranche FR 45.07500 5.38528 H_049 T_081 G_073 JQ921084 JALLIFIE_0_13 Choranche FR 45.07500 5.38528 H_050 T_081 G_073 JQ921085 MITTENA_200906_1ID Mittenwald DE 47.42670 11.25920 H_054 T_081 G_073 JQ921089 MITTENB_200910_1ID Mittenwald DE 47.42670 11.25920 H_055 T_081 G_073 JQ921090 MITTENB_200910_3ex Mittenwald DE 47.42670 11.25920 H_054 T_081 G_073 JQ921091 NEBLONCO_200807_3ex Jenneret BE 50.41377 5.45694 H_056 T_081 G_073 JQ921092 OGOF_200308_4ID Glyntawe GB 51.82441 -3.67296 H_057 T_081 G_073 JQ921093 OGOF_200402_1IDex Glyntawe GB 51.82441 -3.67296 H_047 T_081 G_073 JQ921100 ROCHE_200902_1 Rencruel FR 45.07167 5.49750 H_061 T_081 G_073 JQ921097 ROSSFELD_200911_1ID Rossfeld FR 48.33390 7.63116 H_051 T_081 G_074 JQ921086 SAUVEGAR_0_10ID Sauve FR 43.94056 3.95000 H_062 T_081 G_073 JQ921099 SAUVEGAR_0_9ID Sauve FR 43.94056 3.95000 H_062 T_081 G_073 JQ921098 STEREINE_200807_1ID Pierre-la-Treiche FR 48.64528 5.94194 H_058 T_081 G_073 JQ921094 STEREINE_200807_2 Pierre-la-Treiche FR 48.64528 5.94194 H_059 T_081 G_073 JQ921095 STEREINE_200807_3 Pierre-la-Treiche FR 48.64528 5.94194 H_060 T_081 G_073 JQ921096 SWILDON_200904_2ID Priddy GB 51.25896 -2.67324 H_063 T_081 G_073 JQ921101 TYNINGS_200612_1 Tyning GB 51.30407 -2.75980 H_064 T_081 G_073 JQ921102 UNTERACH_201009_2ex Mondsee AT 47.79230 13.48452 H_065 T_081 G_073 JQ921103 WALDEGG_201009_1ex Interlaken CH 46.71456 7.83211 H_066 T_081 G_075 JQ921104 WEISSEN1_201009_1ID Mondsee AT 47.78919 13.55226 H_067 T_081 G_073 JQ921105 WEISSEN1_201009_2 Mondsee AT 47.78919 13.55226 H_068 T_081 G_073 JQ921106 WEISSEN1_201009_3 Mondsee AT 47.78919 13.55226 H_069 T_081 G_073 JQ921107 Proasellus chappuisi Henry & Magniez, 1968 ARTZEGI_200906_1ID Cigoitia ES 43.01902 -2.75427 H_079 T_087 G_103 JQ921120 ARTZEGI_200906_2 Cigoitia ES 43.01902 -2.75427 H_080 T_087 G_103 JQ921121 ARTZEGI_200906_3 Cigoitia ES 43.01902 -2.75427 H_080 T_087 G_103 JQ921122 Proasellus chauvini Henry & Magniez, 1978 HAYTZA_200811_1ID Saint-Etienne-de-Baigorry FR 43.22222 -1.34528 H_081 T_131 G_189 JQ921123 HAYTZA_200811_2 Saint-Etienne-de-Baigorry FR 43.22222 -1.34528 H_081 T_131 G_189 JQ921124 HAYTZA_200811_3 Saint-Etienne-de-Baigorry FR 43.22222 -1.34528 H_081 T_131 G_189 JQ921125 OYANBELT_200811_1 Saint-Michel FR 43.05889 -1.22806 H_082 T_131 G_112 JQ921126 OYANBELT_200811_2 Saint-Michel FR 43.05889 -1.22806 H_083 T_131 G_112 JQ921127 OYANBELT_200811_3ID Saint-Michel FR 43.05889 -1.22806 H_082 T_131 G_112 JQ921128 Proasellus claudei Henry & Magniez, 1996 VINCENT_201011_1ID Saint-Vincent-Rive-d’Olt FR 44.45945 1.29973 H_084 T_098 G_025 JQ921129 VINCENT_201011_2 Saint-Vincent-Rive-d’Olt FR 44.45945 1.29973 H_085 T_098 G_025 JQ921130 Proasellus coiﬀaiti (Henry & Magniez, 1972) NAVAILLO_200906_1ID Navaillan FR 43.56129 -1.05223 H_086 T_044 G_063 JQ921131 NAVAILLO_200906_2ID Navaillan FR 43.56129 -1.05223 H_087 T_044 G_063 JQ921132 NAVAILLO_200906_3 Navaillan FR 43.56129 -1.05223 H_087 T_044 G_063 JQ921133 Proasellus comasi Henry & Magniez, 1982 VELEZ_200910_1 Vinuela ES 36.85189 -4.13523 H_088 T_112 G_016 JQ921134 VELEZ_200910_2ID Vinuela ES 36.85189 -4.13523 H_089 T_112 G_016 JQ921135 VELEZ_200910_3 Vinuela ES 36.85189 -4.13523 H_090 T_112 G_016 JQ921136 Proasellus coxalis (Dolfus, 1892) AMUNT_200803_1 Banyuls-sur-Mer FR 42.46861 3.09222 H_113 T_047 G_051 JQ921173 AMUNT_200803_3 Banyuls-sur-Mer FR 42.46861 3.09222 H_105 T_047 G_053 JQ921174 BEITED_200909_1ID Lahbiye LB 33.70255 35.47058 H_092 T_045 G_050 JQ921141 BEITED_200909_2 Lahbiye LB 33.70255 35.47058 H_092 T_045 G_050 JQ921142 BENAJO1_200910_1ID La Indiana ES 36.75376 -5.20183 H_093 T_047 G_052 JQ921143 BENAJO1_200910_2 La Indiana ES 36.75376 -5.20183 H_094 T_047 G_052 JQ921144 BISRI_200909_1ID Bisri LB 33.58020 35.53548 H_095 T_045 G_050 JQ921145 BUNAR_200402_1ID Island Sipan HR 42.72361 17.87611 H_099 T_047 G_053 JQ921151 BUNAR_200402_2 Island Sipan HR 42.72361 17.87611 H_099 T_047 G_053 JQ921152 BUNAR_200402_3 Island Sipan HR 42.72361 17.87611 H_100 T_047 G_053 JQ921153 CALPONT_200904_1ID Caldine IT 43.82683 11.30172 H_096 T_047 G_051 JQ921146 CALPONT_200904_2 Caldine IT 43.82683 11.30172 H_097 T_047 G_051 JQ921147 CASAMOZA_200810_1ID Lucciana FR 42.51861 9.44222 H_098 T_047 G_051 JQ921148 CASAMOZA_200810_2 Lucciana FR 42.51861 9.44222 H_098 T_047 G_051 JQ921149 CASAMOZA_200810_3 Lucciana FR 42.51861 9.44222 H_098 T_047 G_051 JQ921150 DADES_201004_1 Boumalne Dadaes MA 31.36680 -5.97527 H_111 T_047 G_053 JQ921137 GAJA_200804_1ID Carcasonne FR 43.19722 2.42028 H_101 T_047 G_051 JQ921171

4 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

LAUCHA1_200406_1ID Bad Lauchstadt DE 51.39622 11.98671 H_101 T_047 G_051 JQ921169 LAUCHA2_200406_4 Bad Lauchstadt DE 51.38177 11.95397 H_102 T_047 G_051 JQ921170 LOPAR_200508_1ID Lopar HR 44.83083 14.73194 H_103 T_047 G_053 JQ921154 LOPAR_200508_2 Lopar HR 44.83083 14.73194 H_104 T_047 G_053 JQ921155 MILLAS_200803_1ID Millas FR 42.68056 2.70583 H_105 T_047 G_051 JQ921156 NAUERNAA_200201_1ID Zaadam NL 52.46431 4.76365 H_108 T_047 G_051 JQ921157 NZALA_201004_1ID Sidi Bnour MA 32.62690 -8.41032 H_098 T_047 G_053 JQ921138 NZALA_201004_2 Sidi Bnour MA 32.62690 -8.41032 H_098 T_047 G_053 JQ921139 NZALA_201004_3 Sidi Bnour MA 32.62690 -8.41032 H_098 T_047 G_053 JQ921140 ORFES_200906_1ID Orfes ES 42.17053 2.86924 H_112 T_047 G_051 JQ921158 ORFES_200906_2 Orfes ES 42.17053 2.86924 H_106 T_047 G_051 JQ921159 QUERCIOL_200904_1ID Querciola IT 43.84525 11.32144 H_106 T_047 G_051 JQ921165 RAAN_200800_3ID Hellsingborg SE 55.99900 12.76600 H_091 T_047 G_051 JQ921166 RAAN_200800_8ID Hellsingborg SE 55.99900 12.76600 H_091 T_047 G_051 JQ921167 RAAN_200800_9 Hellsingborg SE 55.99900 12.76600 H_091 T_047 G_051 JQ921168 STENSAN_200811_2ID Bastad SE 56.42800 12.87300 H_091 T_047 G_161 JQ921172 TLEMCEN_200905_1ID Tlemcen DZ 34.77340 -1.25053 H_109 T_047 G_052 JQ921162 TLEMCEN_200905_2 Tlemcen DZ 34.77340 -1.25053 H_110 T_047 G_052 JQ921163 TLEMCEN_200905_3 Tlemcen DZ 34.77340 -1.25053 H_107 T_047 G_052 JQ921164 TRICHONI_200404_1ID Padanassa GR 38.60278 21.55639 H_107 T_046 G_162 JQ921160 TRICHONI_200404_2 Padanassa GR 38.60278 21.55639 H_107 T_046 G_162 JQ921161 Proasellus deminutus (Sket, 1959) DOVJEZ_200910_1ID Dovjez SI 46.11441 14.48526 H_114 T_056 G_067 JQ921175 DOVJEZ_200910_4ex Dovjez SI 46.11441 14.48526 H_115 T_056 G_067 JQ921176 DOVJEZ_200910_8ex Dovjez SI 46.11441 14.48526 H_115 T_056 G_067 JQ921177 MALECNI2_201009_2 Maribor SI 46.55427 15.69569 H_116 T_053 G_062 JQ921178 MALECNI2_201009_3 Maribor SI 46.55427 15.69569 H_117 T_053 G_062 JQ921179 MALECNI2_201009_3ex Maribor SI 46.55427 15.69569 H_120 T_053 G_062 JQ921183 ROSNJA_201009_1ID Rosnja SI 46.43262 15.75602 H_118 T_053 G_062 JQ921180 ROSNJA_201009_2 Rosnja SI 46.43262 15.75602 H_118 T_053 G_062 JQ921181 ROSNJA_201009_3 Rosnja SI 46.43262 15.75602 H_119 T_053 G_062 JQ921182 Proasellus dianae Pesce & Argano, 1985 CLITUNNO_200904_1ID Campello sul Clitunno IT 42.83293 12.76815 H_121 T_102 G_096 JQ921184 CLITUNNO_200904_2 Campello sul Clitunno IT 42.83293 12.76815 H_122 T_102 G_096 JQ921185 CLITUNNO_200904_3 Campello sul Clitunno IT 42.83293 12.76815 H_121 T_102 G_096 JQ921186 Proasellus ebrensis Henry & Magniez, 1992 CERECEDA_200906_1 Cereceda ES 42.80023 -3.49401 H_123 T_089 G_100 JQ921187 MARFIL_200906_1ID Valdenoceda ES 42.86092 -3.59298 H_124 T_090 G_100 JQ921188 RUDRON_201010_1IDex Basconcillos del Tozo ES 42.70230 -3.91793 H_036 T_089 G_098 JQ921070 RUDRON_201010_3ex Basconcillos del Tozo ES 42.70230 -3.91793 H_037 T_089 G_098 JQ921071 Proasellus escolai Henry & Magniez, 1982 DEIFONTE_200910_1ID Deifontes ES 37.32921 -3.58740 H_128 T_116 G_023 JQ921192 DEIFONTE_200910_2 Deifontes ES 37.32921 -3.58740 H_129 T_116 G_023 JQ921193 SALAR_200910_1ID Salar ES 37.14075 -4.06587 H_125 T_114 G_020 JQ921189 SALAR_200910_2 Salar ES 37.14075 -4.06587 H_126 T_114 G_020 JQ921190 SALAR_201010_3cyto Salar ES 37.14075 -4.06587 H_127 T_114 G_020 JQ921191 Proasellus espanoli Henry & Magniez, 1982 ALCAZAR_200910_1ID Jerez del Marquesado ES 37.17591 -3.16663 H_130 T_122 G_018 JQ921194 ALCAZAR_200910_2 Jerez del Marquesado ES 37.17591 -3.16663 H_131 T_122 G_018 JQ921195 ALCAZAR_200910_3 Jerez del Marquesado ES 37.17591 -3.16663 H_130 T_122 G_018 JQ921196 Proasellus faesulanus Messana & Caselli, 1995 MUGNONE_200904_1ID Pian di Mugnone IT 43.81933 11.29332 H_132 T_101 G_021 JQ921197 MUGNONE_200904_2 Pian di Mugnone IT 43.81933 11.29332 H_133 T_101 G_021 JQ921198 MUGNONE_200904_3 Pian di Mugnone IT 43.81933 11.29332 H_134 T_101 G_021 JQ921199 Proasellus franciscoloi (Chappuis, 1955) BANDITO_200907_1ID Roaschia IT 44.29220 7.42741 H_137 T_066 G_070 JQ921203 BANDITO_200907_3 Roaschia IT 44.29220 7.42741 H_138 T_066 G_070 JQ921204 BOSSEA_200801_2ID Bossea IT 44.24167 7.83972 H_135 T_064 G_069 JQ921200 BOSSEA_200801_3 Bossea IT 44.24167 7.83972 H_135 T_064 G_069 JQ921201 ORSO_200801_1ID Ponte di Nava IT 44.11861 7.87444 H_136 T_064 G_069 JQ921202 Proasellus gjorgjevici (Karaman, 1933) Ohrid MK 41.03730 20.71899 H_475 T_149 G_095 DQ305135 ¶ OSOJ1_200909_1ID Pod Osoj MK 40.95107 20.77643 H_139 T_149 G_095 JQ921205

5 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

Proasellus graﬁ Henry & Magniez, 2003 CUEVAVAL_200906_2 Rasines ES 43.29843 -3.41971 H_140 T_091 G_182 JQ921206 SOURVALL_200906_1 Rasines ES 43.29843 -3.41996 H_140 T_091 G_182 JQ921207 Proasellus granadensis Henry & Magniez, 2003 PILAS_200910_1IDex Alhama de Granada ES 37.04646 -4.06266 H_142 T_113 G_203 JQ921211 PILAS_200910_2ex Alhama de Granada ES 37.04646 -4.06266 H_142 T_113 G_203 JQ921212 PILAS_200910_3ex Alhama de Granada ES 37.04646 -4.06266 H_142 T_113 G_203 JQ921213 Proasellus guipuzcoensis Henry & Magniez, 2003 UBAO_200906_1ID Onate ES 43.00262 -2.40459 H_141 T_138 G_192 JQ921208 UBAO_200906_2 Onate ES 43.00262 -2.40459 H_141 T_138 G_192 JQ921209 UBAO_200906_3 Onate ES 43.00262 -2.40459 H_141 T_138 G_192 JQ921210 Proasellus hercegovinensis (Karaman, 1933) BJELUSNI_200008_1ID Zavala BA 42.84516 17.97832 H_144 T_051 G_163 JQ921215 BJELUSNI_200008_2ID Zavala BA 42.84516 17.97832 H_144 T_051 G_163 JQ921216 LEDENJA_200508_2 Foca BA 43.52333 18.66472 H_143 T_050 G_164 JQ921214 Proasellus hermallensis (Arcangeli, 1938) CHAMPP_200807_2ID Yvoir BE 50.31028 4.88866 H_145 T_104 G_117 JQ921217 CRUPET_200807_5ex Assesse BE 50.35449 4.96145 H_145 T_104 G_117 JQ921218 LUNETTE_200807_1ID Wavreille BE 50.12577 5.25159 H_145 T_104 G_117 JQ921219 LUNETTE_200807_2 Wavreille BE 50.12577 5.25159 H_145 T_104 G_117 JQ921220 LUNETTE_200807_3 Wavreille BE 50.12577 5.25159 H_145 T_104 G_117 JQ921221 NEBLONPR_200807_1ID Himbe BE 50.41642 5.45530 H_145 T_104 G_117 JQ921222 NEBLONPR_200807_2 Himbe BE 50.41642 5.45530 H_146 T_104 G_117 JQ921223 Proasellus ibericus (Braga, 1946) BARRIO_201010_1ID Barrio PT 41.84607 -8.56764 H_147 T_118 G_199 JQ921224 BARRIO_201010_2 Barrio PT 41.84607 -8.56764 H_147 T_118 G_199 JQ921225 BARRIO_201010_3ID Barrio PT 41.84607 -8.56764 H_147 T_118 G_199 JQ921226 Proasellus intermedius (Sket, 1965) COMARIE_200908_1ID Doberdo del Lago IT 45.84420 13.53940 H_154 T_055 G_064 JQ921229 COMARIE_200908_2 Doberdo del Lago IT 45.84420 13.53940 H_148 T_055 G_064 JQ921230 COMARIE_200908_3 Doberdo del Lago IT 45.84420 13.53940 H_149 T_055 G_064 JQ921231 IDREIJCA_200908_1ID Trebusa SI 46.09520 13.83370 H_150 T_055 G_064 JQ921235 IDRIJA_200904_1ID Idrija SI 46.15391 13.77397 H_151 T_055 G_064 JQ921227 IDRIJA_200904_2 Idrija SI 46.15391 13.77397 H_152 T_055 G_064 JQ921228 ZALOSCE_201008_1ID Zalosce SI 45.88808 13.74331 H_153 T_055 G_064 JQ921232 ZALOSCE_201008_2 Zalosce SI 45.88808 13.74331 H_153 T_055 G_064 JQ921233 ZALOSCE_201008_3 Zalosce SI 45.88808 13.74331 H_153 T_055 G_064 JQ921234 Proasellus istrianus (Stammer, 1932) GLINSCIC_200701_1ID Bagnoli IT 45.62051 13.87228 H_158 T_106 G_007 JQ921239 GLINSCIC_200701_2 Bagnoli IT 45.62051 13.87228 H_159 T_106 G_007 JQ921240 GLINSCIC_200701_3 Bagnoli IT 45.62051 13.87228 H_160 T_106 G_007 JQ921241 KLUZA_201007_1ID Trieste IT 45.60010 13.85440 H_161 T_106 G_007 JQ921242 KLUZA_201007_2 Trieste IT 45.60010 13.85440 H_159 T_106 G_007 JQ921243 KLUZA_201007_3 Trieste IT 45.60010 13.85440 H_162 T_106 G_007 JQ921244 STEPAN1_201009_1 Stepani SI 45.55022 13.85564 H_155 T_106 G_007 JQ921236 STEPAN2_201009_2 Stepani SI 45.55225 13.85515 H_156 T_106 G_007 JQ921237 STEPAN2_201009_3 Stepani SI 45.55225 13.85515 H_157 T_106 G_007 JQ921238 Proasellus jaloniacus Henry & Magniez, 1978 JALON_200910_1ID Benichembla ES 38.75669 -0.10486 H_163 T_108 G_008 JQ921245 JALON_200910_2 Benichembla ES 38.75669 -0.10486 H_163 T_108 G_008 JQ921246 JALON_200910_3 Benichembla ES 38.75669 -0.10486 H_164 T_108 G_008 JQ921247 Proasellus karamani (Remy, 1934) ZALOMSKA_200305_1 Nevesinje BA 43.18116 18.12157 H_165 T_038 G_165 JQ921248 Proasellus lagari Henry & Magniez, 1982 CRUZ_200910_1ID Caravaca de la Cruz ES 38.10056 -1.82796 H_166 T_125 G_014 JQ921249 CRUZ_200910_2 Caravaca de la Cruz ES 38.10056 -1.82796 H_167 T_125 G_014 JQ921250 CRUZ_200910_3 Caravaca de la Cruz ES 38.10056 -1.82796 H_168 T_125 G_014 JQ921251 Proasellus lescherae Henry & Magniez, 1978 ALGAS_200910_1ID Arnes ES 40.90855 0.24851 H_169 T_136 G_109 JQ921252 ALGAS_200910_2 Arnes ES 40.90855 0.24851 H_170 T_136 G_109 JQ921253 AQUERRET_200906_2ex Esteribar ES 42.89463 -1.55449 H_174 T_137 G_111 JQ921113 AQUERRET_200906_3 Esteribar ES 42.89463 -1.55449 H_174 T_137 G_111 JQ921114 BERGANT_200910_1ID Villores ES 40.66946 -0.19280 H_171 T_133 G_188 JQ921257 BERGANT_200910_2 Villores ES 40.66946 -0.19280 H_172 T_133 G_188 JQ921258

6 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

LUMBIER_200906_1ID Urraul Bajo ES 42.68482 -1.30277 H_173 T_135 G_108 JQ921254 LUMBIER_200906_2 Urraul Bajo ES 42.68482 -1.30277 H_181 T_135 G_108 JQ921255 LUMBIER_200906_3 Urraul Bajo ES 42.68482 -1.30277 H_181 T_135 G_108 JQ921256 MATARRA_200910_1ID Torre del Compte ES 40.92964 0.10431 H_182 T_136 G_109 JQ921265 MATARRA_200910_2 Torre del Compte ES 40.92964 0.10431 H_183 T_136 G_109 JQ921266 MIJARES_200910_1ID Espadilla ES 40.03000 -0.35000 H_175 T_134 G_107 JQ921267 MIJARES_200910_2 Espadilla ES 40.03000 -0.35000 H_176 T_134 G_107 JQ921268 MONZON_200906_1ID Monzon ES 41.91594 0.17631 H_177 T_132 G_106 JQ921259 MONZON_200906_2 Monzon ES 41.91594 0.17631 H_184 T_132 G_106 JQ921260 MONZON_200906_3 Monzon ES 41.91594 0.17631 H_185 T_132 G_106 JQ921261 PEQUENO_200910_1ID Espadilla ES 40.02410 -0.35433 H_179 T_134 G_107 JQ921269 PEQUENO_200910_2 Espadilla ES 40.02410 -0.35433 H_180 T_134 G_107 JQ921270 SIPAN_200906_1ID Sipan ES 42.18312 -0.26590 H_178 T_132 G_106 JQ921263 SIPAN_200906_2 Sipan ES 42.18312 -0.26590 H_181 T_132 G_106 JQ921264 SIPANFON_200906_1 Sipan ES 42.18312 -0.26590 H_073 T_132 G_106 JQ921262 TASTAV1_200910_2ex Valderrobres ES 40.88716 0.10522 H_074 T_136 G_109 JQ921271 Proasellus ligusticus Bodon & Argano, 1982 MADIRA_200904_1ID Carrodano IT 44.23552 9.63908 H_186 T_099 G_118 JQ921272 MADIRA_200904_2 Carrodano IT 44.23552 9.63908 H_186 T_099 G_118 JQ921273 MADIRA_200904_3 Carrodano IT 44.23552 9.63908 H_187 T_099 G_118 JQ921274 SORBA_200904_1ID Monaglia IT 44.24783 9.47847 H_188 T_100 G_104 JQ921275 SORBA_200904_2 Monaglia IT 44.24783 9.47847 H_189 T_100 G_104 JQ921276 SORBA_200904_3 Monaglia IT 44.24783 9.47847 H_190 T_100 G_104 JQ921277 Proasellus margaleﬁ Henry & Magniez, 1982 ALBENTOS_200910_1ID Albentosa ES 40.12242 -0.76379 H_191 T_103 G_119 JQ921278 ALBENTOS_200910_2 Albentosa ES 40.12242 -0.76379 H_192 T_103 G_119 JQ921279 CASCAN2_200910_1ID Cascante del Rio ES 40.20431 -1.12963 H_193 T_120 G_120 JQ921026 CASCAN2_200910_2 Cascante del Rio ES 40.20431 -1.12963 H_193 T_120 G_120 JQ921027 EBRON_200910_1 Castielfabib ES 40.14534 -1.31605 H_005 T_120 G_120 JQ921115 MEREMIL_200910_1ID Gestalgar ES 39.59690 -0.84903 H_005 T_119 G_196 JQ921280 MEREMIL_200910_2 Gestalgar ES 39.59690 -0.84903 H_075 T_119 G_196 JQ921281 Proasellus meijersae Henry & Magniez, 2003 MUNDO_200910_1ID Riopar ES 38.48366 -2.36152 H_194 T_124 G_022 JQ921282 MUNDO_200910_2 Riopar ES 38.48366 -2.36152 H_195 T_124 G_022 JQ921283 MUNDO_200910_3 Riopar ES 38.48366 -2.36152 H_195 T_124 G_022 JQ921284 Proasellus meridianus (Racovitza, 1919) BARBOTT_200808_1 Thaire FR 46.05694 -0.97639 H_196 T_151 G_004 JQ921285 BARBOTT_200808_2 Thaire FR 46.05694 -0.97639 H_197 T_151 G_004 JQ921286 BOISFONT_200808_1 Vouhe FR 46.15083 -0.77389 H_198 T_151 G_004 JQ921287 BUSTA_200906_1ID Alfoz de Lloredo ES 43.34920 -4.18103 H_213 T_153 G_006 JQ921308 BUSTA_200906_3 Alfoz de Lloredo ES 43.34920 -4.18103 H_214 T_153 G_006 JQ921309 FAIL_201010_12ID Viseu PT 40.60108 -7.98398 H_204 T_151 G_004 JQ921293 FAIL_201010_13 Viseu PT 40.60108 -7.98398 H_204 T_151 G_004 JQ921294 FAIL_201010_14 Viseu PT 40.60108 -7.98398 H_205 T_151 G_004 JQ921295 FERMENTE_201010_1ID Fermentelos PT 40.55199 -8.49900 H_206 T_151 G_004 JQ921521 FERMENTE_201010_2 Fermentelos PT 40.55199 -8.49900 H_206 T_151 G_004 JQ921522 FERMENTE_201010_3 Fermentelos PT 40.55199 -8.49900 H_206 T_151 G_004 JQ921523 GEORGES_201009_1ID Montvalent FR 44.88967 1.61851 H_211 T_151 G_003 JQ921296 GEORGES_201009_2 Montvalent FR 44.88967 1.61851 H_211 T_151 G_003 JQ921297 GEORGES_201009_3 Montvalent FR 44.88967 1.61851 H_212 T_151 G_003 JQ921298 ILESNOUV_200804_1ID Balan FR 45.81583 5.10056 H_207 T_151 G_004 JQ921305 ILESNOUV_200804_2 Balan FR 45.81583 5.10056 H_208 T_151 G_004 JQ921306 ILESNOUV_200804_3 Balan FR 45.81583 5.10056 H_209 T_151 G_004 JQ921307 LAFINOU_201011_1ID Montvalent FR 44.87955 1.61442 H_209 T_151 G_003 JQ921299 LAFINOU_201011_2 Montvalent FR 44.87955 1.61442 H_199 T_151 G_003 JQ921300 LOMBARD_201011_1ID Montvalent FR 44.88191 1.61637 H_200 T_151 G_003 JQ921301 LOMBARD_201011_2 Montvalent FR 44.88191 1.61637 H_201 T_151 G_003 JQ921302 MOULINCH_200905_1ID Gradignan FR 44.76194 -0.63472 H_215 T_151 G_004 JQ921288 MOULINCH_200905_2 Gradignan FR 44.76194 -0.63472 H_216 T_151 G_004 JQ921289 MOULINCH_200905_3 Gradignan FR 44.76194 -0.63472 H_217 T_151 G_004 JQ921290 NAUERNAA_200201_1ID Zaadam NL 52.46431 4.76365 H_210 T_151 G_004 JQ921310

7 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

NAUERNAA_200201_2 Zaadam NL 52.46431 4.76365 H_210 T_151 G_004 JQ921311 NAUERNAA_200201_3 Zaadam NL 52.46431 4.76365 H_220 T_151 G_004 JQ921312 PADIDEBA_201011_1ID Padirac FR 44.85809 1.75047 H_221 T_151 G_003 JQ921303 PADIDEBA_201011_2cyto Padirac FR 44.85809 1.75047 H_202 T_151 G_003 JQ921304 PIEZA_200906_1 Quintanilla de Pienza ES 43.00429 -3.48502 H_203 T_152 G_005 JQ921316 PIEZA_200906_3 Quintanilla de Pienza ES 43.00429 -3.48502 H_218 T_152 G_005 JQ921317 PLAULT_200808_1ID Lureuil FR 46.74694 1.02889 H_215 T_151 G_004 JQ921291 PLAULT_200808_2 Lureuil FR 46.74694 1.02889 H_219 T_151 G_004 JQ921292 POSTLAKE_200912_1ID Clyst Saint George GB 50.68643 -3.41774 H_222 T_151 G_004 JQ921313 POSTLAKE_200912_2 Clyst Saint George GB 50.68643 -3.41774 H_225 T_151 G_004 JQ921314 POSTLAKE_200912_3 Clyst Saint George GB 50.68643 -3.41774 H_225 T_151 G_004 JQ921315 RIBELLE_200808_1 Lureuil FR 46.75194 1.03556 H_225 T_151 G_004 JQ921318 SAVONRU_200812_1ID Savonnieres FR 47.34556 0.55194 H_223 T_151 G_004 JQ921322 SAVONRU_200812_2 Savonnieres FR 47.34556 0.55194 H_224 T_151 G_004 JQ921323 SAVONRU_200812_3 Savonnieres FR 47.34556 0.55194 H_223 T_151 G_004 JQ921324 SOUGEAL_0_1ID Sougeal FR 48.51143 -1.50631 H_359 T_151 G_004 JQ921319 SOUGEAL_0_2 Sougeal FR 48.51143 -1.50632 H_360 T_151 G_004 JQ921320 SOUGEAL_0_3ID Sougeal FR 48.51143 -1.50633 H_360 T_151 G_004 JQ921321 Proasellus micropectinatus Baratti & Messana, 1990 MARGHE_200904_1ID Sesta Godano IT 44.27222 9.65710 H_226 T_086 G_181 JQ921325 MARGHE_200904_2 Sesta Godano IT 44.27222 9.65710 H_226 T_086 G_181 JQ921326 MARGHE_200904_3 Sesta Godano IT 44.27222 9.65710 H_226 T_086 G_181 JQ921327 Proasellus n. sp. (Alviela) ALVIELA_201010_1ID Alcacena PT 39.44561 -8.71215 H_232 T_127 G_012 JQ921337 ALVIELA_201010_3 Alcacena PT 39.44561 -8.71215 H_233 T_127 G_012 JQ921338 Proasellus n. sp. (Aquamalas Pontones) AQUALA_200910_1ID Pontones ES 38.06000 -2.82000 H_070 T_121 G_202 JQ921108 AQUALA_200910_2 Pontones ES 38.06000 -2.82000 H_070 T_121 G_202 JQ921109 AQUALA_200910_3 Pontones ES 38.06000 -2.82000 H_070 T_121 G_202 JQ921110 Proasellus n. sp. (Aquerreta) AQUERRET_200906_1IDex Esteribar ES 42.89463 -1.55449 H_234 T_139 G_115 JQ921339 AQUERRET_200906_4ex Esteribar ES 42.89463 -1.55449 H_235 T_139 G_115 JQ921340 Proasellus n. sp. (Boreon) BOREO_200904_1ID Saint-Martin-Vesubie FR 44.07898 7.25068 H_236 T_077 G_087 JQ921341 BOREO_200904_2 Saint-Martin-Vesubie FR 44.07898 7.25068 H_237 T_077 G_087 JQ921342 BOREO_200904_3 Saint-Martin-Vesubie FR 44.07898 7.25068 H_236 T_077 G_087 JQ921343 SAUTINEE_200904_2 Saint-Sauveur-sur-Tinee FR 44.08073 7.10510 H_238 T_076 G_180 JQ921345 STMART2_200904_1 Saint-Martin-Vesubie FR 44.08318 7.24472 H_236 T_077 G_087 JQ921344 Proasellus n. sp. (Cantarana) CANTARA_200801_1IDex Ponte di Nava IT 44.12111 7.87639 H_001 T_072 G_090 JQ921017 CANTARA_200801_2 Ponte di Nava IT 44.12111 7.87639 H_002 T_072 G_090 JQ921018 CANTARA_200801_4ID Ponte di Nava IT 44.12111 7.87639 H_002 T_072 G_090 JQ921019 Proasellus n. sp. (Lentegi) LENTIGI_201010_1ID Lentegi ES 36.83188 -3.68367 H_239 T_111 G_015 JQ921346 LENTIGI_201010_2 Lentegi ES 36.83188 -3.68367 H_240 T_111 G_015 JQ921347 LENTIGI_201010_3 Lentegi ES 36.83188 -3.68367 H_241 T_111 G_015 JQ921348 Proasellus n. sp. (Maglia) MAGLIA_200908_1ID Breil-sur-Roya FR 43.97152 7.47518 H_242 T_065 G_169 JQ921349 Proasellus n. sp. (Martino) CRISSOLO_200801_1ID Crissolo IT 44.69806 7.14167 H_243 T_061 G_170 JQ921350 CRISSOLO_200801_2ID Crissolo IT 44.69806 7.14167 H_243 T_061 G_170 JQ921351 CRISSOLO_200801_3 Crissolo IT 44.69806 7.14167 H_243 T_061 G_170 JQ921352 Proasellus n. sp. (Mazandaran) MAZANDAR_200910_1 Mahdiye IR 35.67900 51.18656 H_231 T_037 G_166 JQ921336 Proasellus n. sp. (Mescla) MESCLA_200904_20 Villars-sur-Var FR 43.91250 7.18583 H_244 T_063 G_071 JQ921353 MESCLA_200904_21 Villars-sur-Var FR 43.91250 7.18583 H_245 T_063 G_071 JQ921354 Proasellus n. sp. (Pesio Santa Anna) ANNA_200904_1ID Chiusa di Pesio IT 44.31344 7.67890 H_404 T_075 G_089 JQ921579 ANNA_200904_2ID Chiusa di Pesio IT 44.31344 7.67890 H_405 T_075 G_089 JQ921580 ANNA_200904_3 Chiusa di Pesio IT 44.31344 7.67890 H_404 T_075 G_089 JQ921581 Proasellus n. sp. (Sanguiniere) SANGUINI_200908_1ID Entraunes FR 44.25226 6.77112 H_246 T_062 G_171 JQ921355 Proasellus n. sp. (Sospel) SOSPEL_200908_1ID Sospel FR 43.89310 7.42103 H_247 T_073 G_092 JQ921356

8 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

SOSPEL_200908_4 Sospel FR 43.89310 7.42103 H_248 T_073 G_092 JQ921357 Proasellus n. sp. (Verclause) LAPENNE_201002_1ID La Penne-sur-l’Ouveze FR 44.24240 5.22722 H_251 T_071 G_068 JQ921361 VAUCLUS_200703_2ex Fontaine-de-Vaucluse FR 43.91861 5.13194 H_250 T_070 G_168 JQ921359 VAUCLUS_200703_5ex Fontaine-de-Vaucluse FR 43.91861 5.13194 H_250 T_070 G_168 JQ921360 VERCLAUS_201002_1ID Verclause FR 44.38028 5.42590 H_249 T_071 G_068 JQ921358 Proasellus navarrensis Henry & Magniez, 2003 NACEDERO_200906_1ID Larraun ES 42.97672 -1.91840 H_227 T_093 G_183 JQ921328 NACEDERO_200906_2 Larraun ES 42.97672 -1.91840 H_227 T_093 G_183 JQ921329 NACEDERO_200906_3 Larraun ES 42.97672 -1.91840 H_227 T_093 G_183 JQ921330 Proasellus nolli (Karaman, 1952) BURKH1_200905_1 Burkheim DE 48.09162 7.59935 H_228 T_040 G_054 JQ921331 OPFING1_200905_1 Opfingen DE 48.00093 7.75635 H_228 T_040 G_054 JQ921333 OPFING2_200905_1 Opfingen DE 48.00713 7.76364 H_228 T_040 G_054 JQ921334 OPFING3_200905_1ID Opfingen DE 48.00946 7.76707 H_230 T_040 G_054 JQ921335 ROSSFELD_200911_1ID Rossfeld FR 48.33390 7.63116 H_229 T_040 G_054 JQ921332 Proasellus ortizi Henry & Magniez, 1992 JIVERO2_200906_1ID Ozana ES 43.30385 -3.57186 H_252 T_092 G_184 JQ921362 JIVERO2_200906_2 Ozana ES 43.30385 -3.57186 H_252 T_092 G_184 JQ921363 JIVERO2_200906_3 Ozana ES 43.30385 -3.57186 H_252 T_092 G_184 JQ921364 Proasellus oviedensis Henry & Magniez, 2003 QUINTANA_200906_1 La Pereda ES 43.40031 -4.77120 H_253 T_105 G_197 JQ921365 Proasellus parvulus (Sket, 1960) OTOVEC_201009_1ID Crnomelj SI 45.59231 15.16690 H_254 T_048 G_160 JQ921366 OTOVEC_201009_2 Crnomelj SI 45.59231 15.16690 H_254 T_048 G_160 JQ921367 STOBE_201009_1ID Crnomelj SI 45.60372 15.17089 H_254 T_048 G_160 JQ921368 Proasellus pavani (Arcangeli, 1942) STERPO_201008_1ID Bertiolo IT 45.90291 13.04147 H_255 T_052 G_061 JQ921369 STERPO_201008_2 Bertiolo IT 45.90291 13.04147 H_256 T_052 G_061 JQ921370 STERPO_201008_3 Bertiolo IT 45.90291 13.04147 H_257 T_052 G_061 JQ921371 Proasellus racovitzai Henry & Magniez, 1972 BERNATAS_200811_3ID Arbas FR 42.97306 0.89889 H_258 T_110 G_205 JQ921372 Proasellus rectangulatus Afonso, 1982 MONTEMOR_201010_1ID Montemor-o-Novo PT 38.65417 -8.22641 H_259 T_129 G_011 JQ921373 MONTEMOR_201010_2 Montemor-o-Novo PT 38.65417 -8.22641 H_259 T_129 G_011 JQ921374 MONTEMOR_201010_3 Montemor-o-Novo PT 38.65417 -8.22641 H_260 T_129 G_011 JQ921375 Proasellus rectus Afonso, 1982 EVORA_201010_1ID Evora PT 38.60359 -7.87318 H_261 T_130 G_010 JQ921376 EVORA_201010_2 Evora PT 38.60359 -7.87318 H_261 T_130 G_010 JQ921377 EVORA_201010_3 Evora PT 38.60359 -7.87318 H_262 T_130 G_010 JQ921378 Proasellus remyi (Monod, 1932) Ohrid MK 41.03730 20.71899 H_264 T_149 G_094 DQ305130 ¶ NAUMOHRI_200404_1ID Sv Naum MK 40.91028 20.74778 H_265 T_148 G_093 JQ921382 OSOJ2_200909_1ID Pod Osoj MK 40.95107 20.77643 H_263 T_149 G_094 JQ921379 OSOJ3_200909_2ID Pod Osoj MK 40.95107 20.77643 H_263 T_149 G_094 JQ921380 TRPEJCA_200909_1 Trpejca MK 40.96057 20.78289 H_472 T_148 G_093 JQ921381 Proasellus rouchi Henry, 1980 MESCLA_200904_1ID Villars-sur-Var FR 43.91250 7.18583 H_266 T_082 G_178 JQ921383 MESCLA_200904_2ID Villars-sur-Var FR 43.91250 7.18583 H_266 T_082 G_178 JQ921384 MESCLA_200904_5 Villars-sur-Var FR 43.91250 7.18583 H_266 T_082 G_178 JQ921385 Proasellus slavus (Remy, 1948) CEPKI_201009_1ID Cepki SI 45.54328 13.83449 H_267 T_043 G_058 JQ921386 CEPKI_201009_2ID Cepki SI 45.54328 13.83449 H_268 T_043 G_058 JQ921387 CEPKI_201009_3 Cepki SI 45.54328 13.83449 H_268 T_043 G_058 JQ921388 DROBTINC_201009_1ID Apace SI 46.69689 15.84603 H_269 T_041 G_056 JQ921389 DROBTINC_201009_2 Apace SI 46.69689 15.84603 H_270 T_041 G_056 JQ921390 DROBTINC_201009_3 Apace SI 46.69689 15.84603 H_271 T_041 G_056 JQ921391 GLANBACH_201009_1ID Salzburg AT 47.81010 13.02193 H_278 T_040 G_054 JQ921400 GLANBACH_201009_2 Salzburg AT 47.81010 13.02193 H_278 T_040 G_054 JQ921401 GLANBACH_201009_3 Salzburg AT 47.81010 13.02193 H_279 T_040 G_054 JQ921402 GOLLING_201009_1ID Salzburg AT 47.58191 13.17079 H_278 T_040 G_054 JQ921403 GOLLING_201009_2 Salzburg AT 47.58191 13.17079 H_280 T_040 G_054 JQ921404 GOLLING_201009_3 Salzburg AT 47.58191 13.17079 H_281 T_040 G_054 JQ921405 KLUZA_201007_1ID Trieste IT 45.60010 13.85440 H_273 T_042 G_057 JQ921410

9 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

KLUZA_201007_2 Trieste IT 45.60010 13.85440 H_274 T_042 G_057 JQ921411 KLUZA_201007_3 Trieste IT 45.60010 13.85440 H_282 T_042 G_057 JQ921412 LAHAU_200703_1ID Vienne AT 48.18430 16.52240 H_275 T_040 G_054 JQ921393 LAHAU_200703_2 Vienne AT 48.18430 16.52240 H_276 T_040 G_054 JQ921394 MALECNI1_201009_1ex Maribor SI 46.55428 15.69496 H_272 T_041 G_056 JQ921406 MITTENB_200910_1ID Mittenwald DE 47.42670 11.25920 H_269 T_040 G_054 JQ921395 MITTENB_200910_2 Mittenwald DE 47.42670 11.25920 H_283 T_040 G_054 JQ921396 NEVESIN_200504_1 Landau DE 49.19361 8.11361 H_284 T_040 G_054 JQ921392 PRVOVERZ_201009_1ID Verzej SI 46.58587 16.16869 H_277 T_041 G_056 JQ921407 PRVOVERZ_201009_2 Verzej SI 46.58587 16.16869 H_277 T_041 G_056 JQ921408 PRVOVERZ_201009_3 Verzej SI 46.58587 16.16869 H_277 T_041 G_056 JQ921409 SWABIANA_200904_1ID Bachingen an der Brenz DE 48.53250 10.28500 H_285 T_040 G_054 JQ921397 SWABIANA_200904_2 Bachingen an der Brenz DE 48.53250 10.28500 H_286 T_040 G_054 JQ921398 SWABIANA_200904_3 Bachingen an der Brenz DE 48.53250 10.28500 H_287 T_040 G_054 JQ921399 Proasellus slovenicus (Sket, 1957) JAMAPOD_200409_1 Precna SI 45.81886 15.09865 H_288 T_054 G_060 JQ921413 JAMAPOD_200409_2ID Precna SI 45.81886 15.09865 H_289 T_054 G_060 JQ921414 JAMAPOD_200409_3 Precna SI 45.81886 15.09865 H_290 T_054 G_060 JQ921415 Proasellus sp. (type locality of P. gourbaultae) CAUTAB_200910_1ex Jarafuel ES 39.14380 -1.04686 H_071 T_123 G_017 JQ921111 CAUTAB_200910_4ex Jarafuel ES 39.14380 -1.04686 H_072 T_123 G_017 JQ921112 Proasellus spelaeus (Racovitza, 1922) ETCHEVE_200811_1ex Ancille FR 43.13806 -1.20028 H_291 T_142 G_193 JQ921416 ETCHEVE_200811_2ex Ancille FR 43.13806 -1.20028 H_291 T_142 G_193 JQ921417 ETCHEVE_200811_3IDex Ancille FR 43.13806 -1.20028 H_291 T_142 G_193 JQ921418 Proasellus stocki Henry & Magniez, 2003 SAJA_201010_1ID Cabezon de la Sal ES 43.12104 -4.29230 H_076 T_088 G_101 JQ921116 SAJA_201010_2 Cabezon de la Sal ES 43.12104 -4.29230 H_076 T_088 G_101 JQ921117 SAJA_201010_3 Cabezon de la Sal ES 43.12104 -4.29230 H_077 T_088 G_101 JQ921118 Proasellus strouhali (Karaman, 1955) COQUILLE_200807_1ID Etalante FR 47.64500 4.76611 H_292 T_068 G_085 JQ921419 COQUILLE_200807_2ID Etalante FR 47.64500 4.76611 H_292 T_068 G_085 JQ921420 CRECEY_200804_1ID Crecey-sur-Tille FR 47.56333 5.12611 H_293 T_068 G_085 JQ921421 CRECEY_200804_2 Crecey-sur-Tille FR 47.56333 5.12611 H_293 T_068 G_085 JQ921422 GAGGENAU_200703_1ID Gaggenau DE 48.80280 8.32654 H_294 T_068 G_083 JQ921423 GAGGENAU_200703_2 Gaggenau DE 48.80280 8.32654 H_295 T_068 G_083 JQ921424 GAGGENAU_200703_3 Gaggenau DE 48.80280 8.32654 H_294 T_068 G_083 JQ921425 GAISBER_201009_1ID Mondsee AT 47.84830 13.33237 H_298 T_068 G_082 JQ921428 GLANBACH_201009_1ID Salzburg AT 47.81010 13.02193 H_304 T_068 G_082 JQ921435 GOLLING_201009_1ID Salzburg AT 47.58191 13.17079 H_305 T_068 G_082 JQ921436 GRANBAUM_201006_1ID Lods FR 47.04332 6.23755 H_306 T_068 G_085 JQ921439 GRANCUIS_201006_2ID La Chatelaine FR 46.87901 5.81454 H_296 T_068 G_084 JQ921119 MARBRERI_200809_1 Perreon FR 46.05750 4.56294 H_297 T_068 G_085 JQ921426 MARBRERI_200809_2ID Perreon FR 46.05750 4.56294 H_299 T_068 G_085 JQ921427 NORGES_200700_2 Norges-la-Ville FR 47.40694 5.07861 H_300 T_068 G_085 JQ921429 PONTMOUL_200707_1 Pont-les-Moulins FR 47.31750 6.36083 H_301 T_067 G_086 JQ921430 SCENOIRE_200707_2 Cusance FR 47.32083 6.43917 H_302 T_067 G_086 JQ921431 SCENOIRE_200707_4ID Cusance FR 47.32083 6.43917 H_306 T_067 G_086 JQ921432 SOURCFIN_201006_1ID Mesnay FR 46.89295 5.80548 H_306 T_068 G_084 JQ921437 SOURLOUE_201006_1ID Ouhans FR 47.01108 6.29944 H_303 T_068 G_084 JQ921438 UNTERACH_201009_1ID Mondsee AT 47.79230 13.48452 H_303 T_068 G_082 JQ921433 UNTERACH_201009_3 Mondsee AT 47.79230 13.48452 H_078 T_068 G_082 JQ921434 Proasellus synaselloides (Henry, 1963) LAPENNE_201002_1ID La Penne-sur-l’Ouveze FR 44.24240 5.22722 H_307 T_084 G_176 JQ921449 SENEZASS_201002_1ID Senez FR 43.91530 6.40837 H_308 T_085 G_076 JQ921440 SENEZASS_201002_2 Senez FR 43.91530 6.40837 H_311 T_085 G_076 JQ921441 THEOULE_200700_4 Meounes-les-Montrieux FR 43.26361 5.95972 H_311 T_083 G_177 JQ921446 THEOULE_200810_2ID Meounes-les-Montrieux FR 43.26361 5.95972 H_309 T_083 G_177 JQ921445 VAUCLUS_200703_1 Fontaine-de-Vaucluse FR 43.91861 5.13194 H_310 T_085 G_076 JQ921442 VAUCLUS_200703_3 Fontaine-de-Vaucluse FR 43.91861 5.13194 H_309 T_085 G_076 JQ921443 VAUCLUS_200703_4ID Fontaine-de-Vaucluse FR 43.91861 5.13194 H_312 T_085 G_076 JQ921444 VIOLETTE_200707_1ID Tenay FR 45.93667 5.53167 H_312 T_085 G_175 JQ921447

10 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

VIOLETTE_200707_2ID Tenay FR 45.93667 5.53167 H_313 T_085 G_175 JQ921448 Proasellus valdensis (Chappuis, 1948) BALME_200709_1 La Balme-les-Grottes FR 45.85222 5.33917 H_315 T_069 G_072 JQ921452 BALME_200709_19ml La Balme-les-Grottes FR 45.85222 5.33917 H_314 T_069 G_072 JQ921450 BALME_200709_20ml La Balme-les-Grottes FR 45.85222 5.33917 H_314 T_069 G_072 JQ921451 BENOITE_0_3 Arith FR 45.70762 6.04234 H_316 T_069 G_072 JQ921453 BERNARD_200802_1 Samoens FR 46.10197 6.77999 H_324 T_069 G_072 JQ921468 CAVALE_200511_27ml Les Deserts FR 45.66472 5.99000 H_316 T_069 G_072 JQ921454 CAVALE_200511_28ml Les Deserts FR 45.66472 5.99000 H_316 T_069 G_072 JQ921455 CAVALE_200511_29ml Les Deserts FR 45.66472 5.99000 H_316 T_069 G_072 JQ921456 CLAUDE_200504_24ml Saint-Claude FR 46.36417 5.87778 H_332 T_069 G_072 JQ921484 CLAUDE_200504_26ml Saint-Claude FR 46.36417 5.87778 H_332 T_069 G_072 JQ921485 CUVES_200906_1ID Sassenage FR 45.20861 5.65861 H_330 T_069 G_072 JQ921480 CUVES_200906_2 Sassenage FR 45.20861 5.65861 H_330 T_069 G_072 JQ921481 CUVES_200906_3 Sassenage FR 45.20861 5.65861 H_330 T_069 G_072 JQ921482 DOUVERA_200908_1ID La Pesse FR 46.31778 5.83389 H_325 T_069 G_072 JQ921469 DOUVERA_200908_2 La Pesse FR 46.31778 5.83389 H_326 T_069 G_072 JQ921470 DOUVERA_200908_3 La Pesse FR 46.31778 5.83389 H_325 T_069 G_072 JQ921471 FALCON_200806_1 La Burbanche FR 45.84215 5.54655 H_318 T_069 G_072 JQ921458 FARDELE_200704_1f Sixt-Fer-a-Cheval FR 46.02472 6.77333 H_320 T_069 G_072 JQ921462 FARDELW_200602_17ml Sixt-Fer-a-Cheval FR 46.02474 6.77320 H_319 T_069 G_072 JQ921459 FARDELW_200602_18ml Sixt-Fer-a-Cheval FR 46.02474 6.77320 H_319 T_069 G_072 JQ921460 FARDELW_200602_22ml Sixt-Fer-a-Cheval FR 46.02474 6.77320 H_319 T_069 G_072 JQ921461 FOULESC_200710_1ID Saint-Claude FR 46.37667 5.89583 H_321 T_069 G_072 JQ921463 FOULESC_200710_3ID Saint-Claude FR 46.37667 5.89583 H_322 T_069 G_072 JQ921464 FURON_200808_1 Engins FR 45.16694 5.61194 H_317 T_069 G_072 JQ921457 HUGBIS_200709_2 Injoux FR 46.03194 5.76917 H_323 T_069 G_072 JQ921465 HUGBIS_200709_3 Injoux FR 46.03194 5.76917 H_323 T_069 G_072 JQ921466 HUGBIS_200709_4 Injoux FR 46.03194 5.76917 H_323 T_069 G_072 JQ921467 PLAGNE_200511_33ml Entremont-le-Vieux FR 45.44028 5.90361 H_327 T_069 G_072 JQ921472 PLAGNE_200511_34ml Entremont-le-Vieux FR 45.44028 5.90361 H_327 T_069 G_072 JQ921473 PLEUREUS_200509_1 Sixt-Fer-a-Cheval FR 46.01222 6.75861 H_328 T_069 G_072 JQ921476 PLEUREUS_200509_13ml Sixt-Fer-a-Cheval FR 46.01222 6.75861 H_328 T_069 G_072 JQ921474 PLEUREUS_200509_14ml Sixt-Fer-a-Cheval FR 46.01222 6.75861 H_328 T_069 G_072 JQ921475 SALLES_200509_15ml Sixt-Fer-a-Cheval FR 46.00139 6.75389 H_329 T_069 G_072 JQ921477 SALLES_200509_16ml Sixt-Fer-a-Cheval FR 46.00139 6.75389 H_329 T_069 G_072 JQ921478 SALLES_200509_7 Sixt-Fer-a-Cheval FR 46.00139 6.75389 H_329 T_069 G_072 JQ921479 SCESIXT_200704_1 Sixt-Fer-a-Cheval FR 46.05669 6.78425 H_331 T_069 G_072 JQ921483 Proasellus vandeli Magniez & Henry, 1969 CHAPGEN_200811_3ID Licq-Atherey FR 43.08333 -0.87278 H_333 T_144 G_195 JQ921486 CHAPGEN_200811_4 Licq-Atherey FR 43.08333 -0.87278 H_333 T_144 G_195 JQ921487 LAMINA_200811_1 Aussurucq FR 43.14528 -0.92583 H_334 T_143 G_194 JQ921488 UTHURRIA_201005_1ID Viodos-Abense-de-Bas FR 43.23386 -0.91735 H_335 T_145 G_116 JQ921489 UTHURRIA_201005_2 Viodos-Abense-de-Bas FR 43.23386 -0.91735 H_336 T_145 G_116 JQ921490 UTHURRIA_201005_3 Viodos-Abense-de-Bas FR 43.23386 -0.91735 H_336 T_145 G_116 JQ921491 Proasellus vulgaris (Sket, 1965) DOVJEZ_200910_1ex Dovjez SI 46.11441 14.48526 H_337 T_057 G_065 JQ921492 DOVJEZ_200910_1ID Dovjez SI 46.11441 14.48526 H_339 T_057 G_065 JQ921494 DOVJEZ_200910_2ex Dovjez SI 46.11441 14.48526 H_338 T_057 G_065 JQ921493 Proasellus walteri (Chappuis, 1948) AZERGUES_200700_1 Ternand FR 45.92845 4.55026 H_340 T_060 G_080 JQ921495 AZERGUES_200700_2ID Ternand FR 45.92845 4.55026 H_340 T_060 G_080 JQ921496 BELMONT_200707_1ID Belmont FR 47.00945 5.59472 H_341 T_059 G_079 JQ921497 BURKH1_200905_1ex Burkheim DE 48.09162 7.59935 H_342 T_058 G_078 JQ921498 COLLIER_200804_1ID Perreon FR 46.06694 4.57778 H_353 T_059 G_079 JQ921510 COLLIER_200804_2 Perreon FR 46.06694 4.57778 H_354 T_059 G_079 JQ921511 COLLIER_200804_3 Perreon FR 46.06694 4.57778 H_353 T_059 G_079 JQ921512 COQUILLE_200807_1ID Etalante FR 47.64500 4.76611 H_343 T_059 G_079 JQ921499 COQUILLE_200807_2ID Etalante FR 47.64500 4.76611 H_344 T_059 G_079 JQ921500 LAPENNE_201002_2ex La Penne-sur-l’Ouveze FR 44.24240 5.22722 H_358 T_060 G_179 JQ921520 LONGAMON_200804_1ID Longvic FR 47.28667 5.07944 H_349 T_059 G_079 JQ921505

11 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

LONGAMON_200804_2 Longvic FR 47.28667 5.07944 H_350 T_059 G_079 JQ921506 LONGAMON_200804_3 Longvic FR 47.28667 5.07944 H_350 T_059 G_079 JQ921507 MONTBAR_200707_1 Montbarrey FR 47.01111 5.62972 H_351 T_059 G_079 JQ921508 MONTBAR_200707_2 Montbarrey FR 47.01111 5.62972 H_352 T_059 G_079 JQ921509 MONTJOU1_201002_1ID Montjoux FR 44.50177 5.08935 H_347 T_060 G_081 JQ921503 MONTJOU1_201002_2 Montjoux FR 44.50177 5.08935 H_348 T_060 G_081 JQ921504 ROSSFELD_200911_1ID Rossfeld FR 48.33390 7.63116 H_356 T_058 G_078 JQ921516 ROSSFELD_200911_2 Rossfeld FR 48.33390 7.63116 H_356 T_058 G_078 JQ921517 SOUSPIRON_200700_2ID L’Isle-sur-la-Sorgue FR 43.90750 5.09000 H_357 T_060 G_080 JQ921518 SOUSPIRON_200700_4 L’Isle-sur-la-Sorgue FR 43.90750 5.09000 H_357 T_060 G_080 JQ921519 VERCLAUS_201002_1IDex Verclause FR 44.38028 5.42590 H_345 T_060 G_081 JQ921501 VERCLAUS_201002_2ex Verclause FR 44.38028 5.42590 H_346 T_060 G_081 JQ921502 VIOLETTE_200707_1ID Tenay FR 45.93667 5.53167 H_355 T_060 G_080 JQ921513 VIOLETTE_200707_2ID Tenay FR 45.93667 5.53167 H_355 T_060 G_080 JQ921514 VIOLETTE_200707_3ID Tenay FR 45.93667 5.53167 H_355 T_060 G_080 JQ921515 Remasellus parvus (Steeves, 1964) Madison US 30.41611 -83.21389 H_494 T_030 G_149 AY566472 § Stenasellus breuili Racovitza, 1924 ALLI_200906_1ID Larraun ES 42.98775 -1.89284 H_423 T_015 G_013 JQ921607 ALLI_200906_2 Larraun ES 42.98775 -1.89284 H_424 T_015 G_013 JQ921608 ALLI_200906_3 Larraun ES 42.98775 -1.89284 H_425 T_015 G_013 JQ921609 Stenasellus buili Remy, 1949 CAUNENG_197112_13 Opoul FR 42.86920 2.87388 H_428 T_011 G_044 JQ921613 CAUNENG_197112_2 Opoul FR 42.86920 2.87388 H_429 T_011 G_044 JQ921614 FONTANIL_200908_1ID Puechabon FR 43.75250 3.62331 H_427 T_012 G_033 JQ921610 FONTANIL_200908_2 Puechabon FR 43.75250 3.62331 H_427 T_012 G_033 JQ921611 CENTFONT_200908_1 Causse-de-la-Selle FR 43.75944 3.62306 H_426 T_012 G_033 JQ921612 Stenasellus galhanoae Braga, 1962 MORA_201010_1ID Aracena ES 37.78570 -6.83335 H_430 T_019 G_024 JQ921615 MORA_201010_3 Aracena ES 37.78570 -6.83335 H_431 T_019 G_024 JQ921616 Stenasellus racovitzai Razzauti, 1925 BERNACCI_200810_1ID Castifao FR 42.54583 9.13722 H_434 T_016 G_026 JQ921619 BERNACCI_200810_2 Castifao FR 42.54583 9.13722 H_435 T_016 G_026 JQ921620 CASALUNA_200810_1ID Lano FR 42.38750 9.25861 H_438 T_016 G_026 JQ921623 CASALUNA_200810_2 Lano FR 42.38750 9.25861 H_439 T_016 G_026 JQ921624 CASAMOZA_200810_1 Lucciana FR 42.51861 9.44222 H_436 T_017 G_027 JQ921621 CASAMOZA_200810_2ID Lucciana FR 42.51861 9.44222 H_437 T_017 G_027 JQ921622 FIUMALTO_200810_1 Penta-di-Casinca FR 42.44472 9.47306 H_433 T_016 G_026 JQ921618 SOLENZA_200908_1ID Sari-Solenzara FR 41.86620 9.38766 H_440 T_017 G_028 JQ921625 SOLENZA_200908_2 Sari-Solenzara FR 41.86620 9.38766 H_441 T_017 G_028 JQ921626 Stenasellus virei Dollfus, 1897 ALBANYA_200906_1ID Albanya ES 42.30525 2.72037 H_460 T_013 G_001 JQ921629 ALBENTOS_200910_1ID Albentosa ES 40.12242 -0.76379 H_461 T_182 G_077 JQ921659 ALBIA_200807_1 Llorengoz ES 42.98220 -3.10021 H_442 T_004 G_131 JQ921617 ALGAS_200910_1ID Arnes ES 40.90855 0.24851 H_443 T_003 G_099 JQ921651 ALTURA_200910_1ID Altura ES 39.83925 -0.50857 H_443 T_181 G_129 JQ921660 BERGANT_200910_1ID Villores ES 40.66946 -0.19280 H_444 T_003 G_099 JQ921652 CERECEDA_200906_1ID Cereceda ES 42.80023 -3.49401 H_444 T_002 G_110 JQ921635 CERECEDA_200906_2FID Cereceda ES 42.80023 -3.49401 H_446 T_002 G_110 JQ921636 CERECEDA_200906_3 Cereceda ES 42.80023 -3.49401 H_447 T_002 G_110 JQ921637 COBALLE1_200906_1FID Caso ES 43.18619 -5.38696 H_446 T_007 G_122 JQ921664 CONCHA_201010_1ID Cudillero ES 43.55978 -6.19003 H_448 T_008 G_124 JQ921627 CONCHA_201010_2 Cudillero ES 43.55978 -6.19003 H_448 T_008 G_124 JQ921628 CONCHA_201010_2ex Cudillero ES 43.55978 -6.19003 H_448 T_008 G_124 JQ921572 COUMES_200802_1 Riverenet FR 43.01710 1.15327 H_449 T_184 G_066 JQ921647 COUMES_200802_2 Riverenet FR 43.01710 1.15327 H_468 T_184 G_066 JQ921648 CREUS_200906_1ID Santes Creus ES 41.34883 1.36169 H_450 T_183 G_127 JQ921667 CREUS_200906_2 Santes Creus ES 41.34883 1.36169 H_450 T_183 G_127 JQ921668 EBRON_200910_1ID Castielfabib ES 40.14534 -1.31605 H_451 T_001 G_130 JQ921661 FUESENTA_200906_1FID Nava ES 43.34588 -5.48251 H_451 T_009 G_123 JQ921638 FUESENTA_200906_2 Nava ES 43.34588 -5.48251 H_451 T_009 G_123 JQ921639 FUESENTA_200906_3 Nava ES 43.34588 -5.48251 H_398 T_009 G_123 JQ921640

12 Morvan et al. Timetree of Aselloidea reveals species diversiﬁcation dynamics in groundwater Appendix 2

JIVERO2_200906_1FID Ozana ES 43.30385 -3.57186 H_398 T_005 G_088 JQ921641 MATARRA_200910_1ID Torre del Compte ES 40.92964 0.10431 H_398 T_003 G_099 JQ921662 MIJARES_200910_1ID Espadilla ES 40.03000 -0.35000 H_452 T_182 G_077 JQ921650 MONZON_200906_1ID Monzon ES 41.91594 0.17631 H_452 T_003 G_099 JQ921653 MONZON_200906_2 Monzon ES 41.91594 0.17631 H_453 T_003 G_099 JQ921654 MONZON_200906_3 Monzon ES 41.91594 0.17631 H_454 T_003 G_099 JQ921655 NERTNAPP_196802_1 Lacourt FR 42.94460 1.17457 H_432 T_186 G_128 JQ921632 NERTNAPP_196802_2 Lacourt FR 42.94460 1.17457 H_455 T_186 G_128 JQ921633 ORFES_200906_1FID Orfes ES 42.17053 2.86924 H_456 T_013 G_001 JQ921630 ORFES_200906_2 Orfes ES 42.17053 2.86924 H_457 T_013 G_001 JQ921631 OUEILNEE_200811_1IDex Rebenacq FR 43.15000 -0.40472 H_458 T_006 G_055 JQ921657 OUEILNEE_200811_2ex Rebenacq FR 43.15000 -0.40472 H_457 T_006 G_055 JQ921658 PEQUENO_200910_1FID Espadilla ES 40.02410 -0.35433 H_459 T_182 G_077 JQ921663 PROMILHA_0_1 Fayet FR 43.80500 2.91861 H_462 T_185 G_126 JQ921666 PUJOS_196403_1 Pujos FR 43.03400 0.82204 H_463 T_184 G_066 JQ921649 RIBERO_200906_1FID_YLK153 El Ribero ES 43.03771 -3.47997 H_464 T_002 G_110 JQ921665 RIBERO_200906_1FID_YLK280 El Ribero ES 43.03771 -3.47997 H_465 T_002 G_110 JQ921634 SOURVALL_200906_1 Rasines ES 43.29843 -3.41996 H_466 T_005 G_088 JQ921642 SOURVALL_200906_2FID Rasines ES 43.29843 -3.41996 H_467 T_005 G_088 JQ921643 TOJOS_201010_1ID Cabezon de la Sal ES 43.15919 -4.25163 H_470 T_010 G_125 JQ921644 TOJOS_201010_2 Cabezon de la Sal ES 43.15919 -4.25163 H_470 T_010 G_125 JQ921645 TOJOS_201010_3 Cabezon de la Sal ES 43.15919 -4.25163 H_469 T_010 G_125 JQ921646 TRILLO_200910_1ID_YCD364 Trillo ES 40.69067 -2.58333 H_445 T_001 G_002 JQ921656 TRILLO_200910_1ID_YCD365 Trillo ES 40.69067 -2.58333 H_471 T_001 G_002 JQ921669 Synasellus bragaianus Henry & Magniez, 1987 DEVAILCE_200906_1 Las Ilces ES 43.11537 -4.76860 H_415 T_165 G_157 JQ921595 DEVANARG_200906_1ex Narganes ES 43.32999 -4.56848 H_413 T_165 G_048 JQ921592 DEVAPANE_200906_1ID Panes ES 43.31075 -4.60950 H_413 T_165 G_048 JQ921593 HERMIDA_200906_1 La Hermida ES 43.25027 -4.60514 H_414 T_165 G_048 JQ921594 YUSOREIN_200906_1 Portilla de la Reina ES 43.02150 -4.86578 H_416 T_168 G_049 JQ921596 YUSOREIN_200906_2ex Portilla de la Reina ES 43.02150 -4.86578 H_417 T_168 G_049 JQ921597 Synasellus meijersae Henry & Magniez, 1987 PENDONES_200906_1ID Caso ES 43.13238 -5.24747 H_418 T_167 G_158 JQ921598 PENDONES_200906_2ID Caso ES 43.13238 -5.24747 H_418 T_167 G_158 JQ921599 PENDONES_200906_3ID Caso ES 43.13238 -5.24747 H_418 T_167 G_158 JQ921600 Synasellus n. sp. (Aboim) ABOIM_201010_1ID Aboim das Chocas PT 41.91711 -8.44715 H_419 T_164 G_047 JQ921601 ABOIM_201010_4ID Aboim das Chocas PT 41.91711 -8.44715 H_420 T_164 G_047 JQ921602 Synasellus n. sp. (Los Tojos) TOJOS_201010_1ID Cabezon de la Sal ES 43.15919 -4.25163 H_421 T_166 G_159 JQ921603 TOJOS_201010_2 Cabezon de la Sal ES 43.15919 -4.25163 H_421 T_166 G_159 JQ921604

∗ Verovnik, R., Sket, B. & Trontelj, P. (2005). The colonization of Europe by the freshwater crustacean Asellus aquaticus (Crustacea : Isopoda) proceeded from ancient refugia and was directed by habitat connectivity. Mol. Ecol., 14, 4355-4369. † Verovnik, R., Sket, B. & Trontelj, P. (2004). Phylogeography of subterranean and surface populations of water lice Asellus aquaticus (Crustacea : Isopoda). Mol. Ecol., 13, 1519-1532. ‡ Osborn, K.J. (2009). Relationships within the Munnopsidae (Crustacea, Isopoda, Asellota) based on three genes. Zool. Scr., 38,617-635. § Krejca, J.K. (2005). Stygobite phylogenetics as a tool for determining aquifer evolution. Ph.D. thesis (University of Texas, Austin). ¶ Wysocka, A., Kostoski, G., Kilikowska, A., Wróbel, B. & Sell, J. (2008). The Proasellus (Crustacea, Isopoda) species group, endemic to the Balkan Lake Ohrid : a case of ecological diversiﬁcation ? Fundam. Appl. Limnol., 172,301-313.