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NATIONAL SHELLFISHERIES ASSOCIATION Program And NATIONAL SHELLFISHERIES ASSOCIATION Program and Abstracts of the 110th Annual Meeng March 18 – 22, 2018 Seale, Washington NSA 110th ANNUAL MEETING National Shellfisheries Association Renaissance Hotel, SEATTLE, WASHINGTON March 18 – March 22, 2018 SUNDAY, MARCH 18, 2018 6:30 PM STUDENT ORIENTATION (Foyer outside Courtyard Ballroom) 7:00 PM PRESIDENT’S RECEPTION Courtyard Ballroom MONDAY, MARCH 19, 2018 6:30-8:00 AM STUDENT BREAKFAST (students only) South 8:00-8:50 AM PLENARY LECTURE: Suzanne Williams (Natural History Museum, London) Courtyard Ballroom Federal Superior Municipal James North SHELLFISH LEASING AND GENETICS, THE SHRIMP OLYMPIA PERMITTING WORKSHOP: 9:00-10:30 AM GENOMICS, AND EPIGENOME OYSTERS CASE STUDIES FOR OVERCOMING SCALLOPS BREEDING (SHRIMPENCODE) COMMON LEGAL BARRIERS PROJECT 10:30-11:00AM MORNING BREAK THE SHRIMP GENETICS, OLYMPIA COLLABORATIVE RESEARCH AND EPIGENOME 11:00-12:30PM GENOMICS, AND SCALLOPS OYSTERS ENGAGING FISHERMEN (SHRIMPENCODE) BREEDING PROJECT 12:30-1:30 PM LUNCH BREAK THE SHRIMP GENETICS, OLYMPIA COLLABORATIVE RESEARCH AND EPIGENOME 1:30-2:15 PM GENOMICS, AND SCALLOPS OYSTERS ENGAGING FISHERMEN (SHRIMPENCODE) BREEDING PROJECT THE SHRIMP GENETICS, OLYMPIA COLLABORATIVE RESEARCH AND EPIGENOME GENERAL 2:15-3:00 PM GENOMICS, AND OYSTERS ENGAGING FISHERMEN (SHRIMPENCODE) CONTRIBUTED I BREEDING PROJECT 3:00-3:30 PM AFTERNOON BREAK THE SHRIMP GENETICS, OLYMPIA COLLABORATIVE RESEARCH AND EPIGENOME GENERAL 3:30-6:00 PM GENOMICS, AND OYSTERS ENGAGING FISHERMEN (SHRIMPENCODE) CONTRIBUTED I BREEDING PROJECT TUESDAY, MARCH 20, 2018 8:00-8:50 AM PLENARY LECTURE: Peter Beninger (Universite de Nantes) Courtyard Ballroom Federal Superior Municipal James North SCIENCE, ART, AND HARMFUL ALGAL GENOMICS: AN GENERAL 9:00-10:30 AM VIBRIO COMMUNICATION BLOOMS INTRODUCTORY CONTRIBUTED II PRIMER WORKSHOP 10:30-11:00AM MORNING BREAK COMPARATIVE GENOMICS: SEAGRASS-BIVALVE HARMFUL ALGAL A HANDS-ON GENERAL 11:00-12:30PM VIBRIO INTERACTIONS BLOOMS WORKSHOP ON THE CONTRIBUTED II EASTERN OYSTER GENOME 12:30-1:30 PM LUNCH BREAK PREPARATION OF NORTHWEST INDIAN SHELLFISH FOR TRIBES SEAGRASS-BIVALVE 1:30-3:15 PM VIBRIO CLAMS DISEASE SHELLFISH INTERACTIONS DIAGNOSTICS MANAGEMENT WORKSHOP AND FISHERIES PREPARATION OF NORTHWEST INDIAN PHYTOPLANKTON SHELLFISH FOR TRIBES SEAGRASS-BIVALVE 3:15-4:30 PM VIBRIO CULTURE: A TRIBUTE DISEASE SHELLFISH INTERACTIONS TO RRL GUILLARD DIAGNOSTICS MANAGEMENT WORKSHOP AND FISHERIES POSTER SESSION AND HAPPY HOUR 4:00-6:00 PM Madison Ballroom STUDENT ENDOWMENT FUND AUCTION 7:00PM -??? Courtyard Ballroom WEDNESDAY, MARCH 21, 2018 8:00-8:50 AM PLENARY LECTURE: Donal Manahan (University of Southern California) Courtyard Ballroom North West James South East UNDERGRADUATE CONTAMINANTS OF OYSTERS: EAST MEETS GENERAL 9:00-10:30 AM GREEN CRABS RESEARCH EMERGING CONCERN SOUTH MEETS WEST CONTRIBUTED III COLLOQUIUM 10:30-11:00AM MORNING BREAK CONTAMINANTS OF OYSTERS: EAST MEETS GENERAL 11:00-11:45PM GREEN CRABS RIBBED MUSSELS EMERGING CONCERN SOUTH MEETS WEST CONTRIBUTED III OYSTERS: EAST MEETS GENERAL 11:45-12:30PM GREEN CRABS RIBBED MUSSELS SHELLS AND MATERIALS SOUTH MEETS WEST CONTRIBUTED III NSA BUSINESS LUNCHEON 12:30-2:00 PM Courtyard Ballroom SCALLOP GALLOP 2:00-4:30 PM EXPLORE SEATTLE!! POSTER SESSION AND HAPPY HOUR 4:30-6:00 PM Madison Ballroom KEN CHEW CHINESE DINNER 6:30 PM-??? (Tickets MUST be purchased by 5 PM on Monday) THURSDAY, MARCH 22, 2018 8:00-8:50 AM PLENARY LECTURE: Gary Wikfors (NOAA/NMFS, Northeast Fisheries Science Center) Courtyard Ballroom Federal Superior Municipal North West APPLICATIONS OF ONE HEALTH CRUSTACEAN EPIGENOMICS AND SHELLFISH AND 9:00-10:15 AM GEODUCKS OsHV-1 BIOLOGY TO MICROBIOMES: CLIMATE CHANGE FISHERIES FROM SOIL TO MANAGEMENT PEOPLE WORKSHOP 10:15-10:45AM MORNING BREAK APPLICATIONS OF ONE HEALTH CRUSTACEAN EPIGENOMICS AND SHELLFISH AND 10:45-12:00PM GEODUCKS OsHV-1 BIOLOGY TO MICROBIOMES: CLIMATE CHANGE FISHERIES FROM SOIL TO MANAGEMENT PEOPLE WORKSHOP 12:00-1:00 PM LUNCH BREAK APPLICATIONS OF ONE HEALTH CRUSTACEAN EPIGENOMICS AND SHELLFISH AND 1:00- 3:30PM DOWN ON THE FARM OsHV-1 BIOLOGY TO MICROBIOMES: CLIMATE CHANGE FISHERIES FROM SOIL TO MANAGEMENT PEOPLE WORKSHOP CLOSING HAPPY HOUR 3:30-6:30PM Madison Ballroom All BREAKS are in the MADISON BALLROOM with the POSTERS. In addition to the scheduled Poster Sessions, posters will be available for viewing in the Madison Ballroom from Monday, 9 a.m. through Thursday noon. PLEASE REMOVE YOUR POSTERS BY NOON ON THURSDAY 12 National Shellfisheries Association Seattle, Washington Abstracts 110th Annual Meeting, March 18–22, 2018 CONTENTS ORAL PRESENTATIONS John Adams RECOGNIZING HOW LNC VARIATIONS EXPOSE OLYMPIA OYSTER POPULATIONS TO THERMAL RISK.................................................................................................................................................. 35 Nicolaus G. Adams, Stephanie K. Moore, John B. Mickett, Tina Mikulski, Gregory J. Doucette, James M. Birch USE OF THE ENVIRONMENTAL SAMPLE PROCESSOR FOR NEAR-REAL TIME DETECTION OF HARMFUL ALGAL SPECIES AND DOMOIC ACID IN THE PACIFIC NORTHWEST .......................... 35 M. Victoria Agnew, Abigail Scro, Roxanna Smolowitz USE OF A QPCR ASSAY TO DETECT THE TREMATODE, PROCTOECES MACULATUS, IN WATER COLUMN SAMPLES ............................................................................................................................ 35 Acacia Alcivar-Warren ONE HEALTH EPIGENOMICS AND MICROBIOMES: FROM SOIL TO PEOPLE WORKSHOP ............... 36 Acacia Alcivar-Warren SHRIMP EPIGENOME (SHRIMPENCODE) PROJECT SESSION .................................................................. 36 Acacia Alcivar-Warren, ShrimpENCODE collaborators BASIC RESEARCH NEEDED TO PRODUCE HEALTHY SHRIMP: A PUBLICLY AVAILABLE, FULLY- ASSEMBLED, REFERENCE GENOME FROM THE FIRST SPECIFIC PATHOGEN-FREE (SPF) DOMESTICATED STOCKS OF PENAEUS (LITOPENAEUS) VANNAMEI IN THE UNITED STATES IS A PRIORITY..................................................................................................................................................... 36 Acacia Alcivar-Warren, ShrimpENCODE collaborators SHRIMPENCODE (2017-2027): TOWARDS UNDERSTANDING THE EPIGENETIC MECHANISMS AND TRANSGENERATIONAL INHERITANCE ASSOCIATED WITH EXPOSURE OF SPF SHRIMP, PENAEUS (LITOPENAEUS) VANNAMEI, TO ACUTE HEPATOPANCREATIC NECROSIS DISEASE (AHPND)-CAUSING VIBRIO SPP. AND MICROBIAL TRANSGENE INSECTICIDE, BACILLUS THURINGIENSIS.................................................................................................................................................. 37 Acacia Alcivar-Warren, ShrimpENCODE Project Collaborators SHRIMPENCODE (2017-2027): TRANSPOSABLE ELEMENTS OF THE ORIGINAL SPECIFIC PATHOGEN-FREE (SPF) SHRIMP, PENAEUS (LITOPENAEUS) VANNAMEI, DOMESTICATED IN THE UNITED STATES – REVIEW OF HORIZONTAL TRANSFER OF TRANSPOSONS (HTT) ASSOCIATED WITH SHRIMP DISEASES............................................................... 37 Acacia Alcivar-Warren, Weidong Bao, Daniela Espinoza THE NONLTR-1_LVA NON-LTR RETROTRANSPON OF SPECIFIC PATHOGEN-FREE (SPF) SHRIMP, LI- TOPENAEUS VANNAMEI, FROM THE UNITED STATES IS HOMOLOGOUS TO THE NLRS NON- LTR RETROTRANSPOSON ASSOCIATED WITH ABDOMINAL SEGMENT DEFORMITY DISEASE (ASDD) OF CULTIVATED SHRIMP FROM THAILAND................................................................................. 38 Acacia Alcivar-Warren, Bruno Gomez-Gil, Sonia Soto-Rodriguez, Zhenkang Xu, Daniela Espinoza TRANSPOSASE DDE DOMAINS AND N-6 DNA METHYLASE FAMILY PROTEINS AS POTENTIAL EPIGENETIC MARKS OF PATHOGENIC VIBRIO SPP. FROM ASIA (CHINA, VIETNAM, THAILAND) AND LATIN AMERICA (MEXICO, ECUADOR) THAT CAUSE ACUTE HEPATOPANCREATIC NECROSIS DISEASE (AHPND) IN SHRIMP.................................................................................................... 38 Diego Alejandro-Tigrero, Karina Alejandro-Rosales, Kelvin Molina-Vasquez, Daniela Espinoza, Ana Lucia Matute, Ryan Hutchins, Allison Berardi, Sruthi Tanikella, Martha Reyes, Ayron Zambrano, Cristhian Argandoña, Miriam Alcivar-Arteaga, Chika Ikeogu, E.T. Asogwa, S.M. Obodoefuna, Chinnaiah Amutha, Karol Alarcon, Sara Zambrano-Basurto, Acacia Alcivar-Warren CHILDRENENCODE (2017-2027): REVIEW OF EPIGENETIC MECHANISMS ASSOCIATED WITH ENDOCRINE DISRUPTING CHEMICALS (GLYPHOSATE, METALS, BISPHENOL A) AND MICROBIAL TRANSGENE BACILLUS THURINGIENSIS IN SHELLFISH AND PEOPLE FROM ESTUARIES: A CASE STUDY OF METALS ASSOCIATED WITH MICROCEPHALY AND OTHER CONGENITAL MALFORMATIONS IN CHILDREN FROM THE SANTA ELENA PENINSULA OF ECUADOR ..................................................................................................................................................... 39 Standish K. Allen, Jr., J.M. Small, Peter D. Kube FAMILY-BASED GENETIC PARAMETERS FOR TETRAPLOID CRASSOSTREA VIRGINICA AND BREEDING IMPLICATIONS.............................................................................................................................. 39 National Shellfisheries Association Seattle, Washington Abstracts 110th Annual Meeting, March 18–22, 2018 13 Lindsay Alma, Courtney Fiamengo, Simone Alin, Molly Jackson, Jacqueline Padilla-Gamino VARIABLE SPATIAL AND TEMPORAL OCEAN CONDITIONS IMPACT PHYSIOLOGICAL PERFORMANCE OF BIVALVES IN PUGET SOUND, WASHINGTON, USA............................................... 40 Joel Anderson, Zachary Olsen, Tom Wagner, Carey Gelpi, Darin Topping ENVIRONMENTAL DRIVERS OF THE SPATIAL AND TEMPORAL DISTRIBUTION OF SPAWNING BLUE CRABS, CALLINECTES SAPIDUS, IN THE WESTERN GULF OF MEXICO....................................
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