Differential Gene Expression Analysis Reveals Novel Genes and Pathways
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Metallothionein Monoclonal Antibody, Clone N11-G
Metallothionein monoclonal antibody, clone N11-G Catalog # : MAB9787 規格 : [ 50 uL ] List All Specification Application Image Product Rabbit monoclonal antibody raised against synthetic peptide of MT1A, Western Blot (Recombinant protein) Description: MT1B, MT1E, MT1F, MT1G, MT1H, MT1IP, MT1L, MT1M, MT2A. Immunogen: A synthetic peptide corresponding to N-terminus of human MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1IP, MT1L, MT1M, MT2A. Host: Rabbit enlarge Reactivity: Human, Mouse Immunoprecipitation Form: Liquid Enzyme-linked Immunoabsorbent Assay Recommend Western Blot (1:1000) Usage: ELISA (1:5000-1:10000) The optimal working dilution should be determined by the end user. Storage Buffer: In 20 mM Tris-HCl, pH 8.0 (10 mg/mL BSA, 0.05% sodium azide) Storage Store at -20°C. Instruction: Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Datasheet: Download Applications Western Blot (Recombinant protein) Western blot analysis of recombinant Metallothionein protein with Metallothionein monoclonal antibody, clone N11-G (Cat # MAB9787). Lane 1: 1 ug. Lane 2: 3 ug. Lane 3: 5 ug. Immunoprecipitation Enzyme-linked Immunoabsorbent Assay ASSP5 MT1A MT1B MT1E MT1F MT1G MT1H MT1M MT1L MT1IP Page 1 of 5 2021/6/2 Gene Information Entrez GeneID: 4489 Protein P04731 (Gene ID : 4489);P07438 (Gene ID : 4490);P04732 (Gene ID : Accession#: 4493);P04733 (Gene ID : 4494);P13640 (Gene ID : 4495);P80294 (Gene ID : 4496);P80295 (Gene ID : 4496);Q8N339 (Gene ID : 4499);Q86YX0 (Gene ID : 4490);Q86YX5 -
Emerging Roles of Metallothioneins in Beta Cell Pathophysiology: Beyond and Above Metal Homeostasis and Antioxidant Response
biology Review Emerging Roles of Metallothioneins in Beta Cell Pathophysiology: Beyond and above Metal Homeostasis and Antioxidant Response Mohammed Bensellam 1,* , D. Ross Laybutt 2,3 and Jean-Christophe Jonas 1 1 Pôle D’endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, B-1200 Brussels, Belgium; [email protected] 2 Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; [email protected] 3 St Vincent’s Clinical School, UNSW Sydney, Sydney, NSW 2010, Australia * Correspondence: [email protected]; Tel.: +32-2764-9586 Simple Summary: Defective insulin secretion by pancreatic beta cells is key for the development of type 2 diabetes but the precise mechanisms involved are poorly understood. Metallothioneins are metal binding proteins whose precise biological roles have not been fully characterized. Available evidence indicated that Metallothioneins are protective cellular effectors involved in heavy metal detoxification, metal ion homeostasis and antioxidant defense. This concept has however been challenged by emerging evidence in different medical research fields revealing novel negative roles of Metallothioneins, including in the context of diabetes. In this review, we gather and analyze the available knowledge regarding the complex roles of Metallothioneins in pancreatic beta cell biology and insulin secretion. We comprehensively analyze the evidence showing positive effects Citation: Bensellam, M.; Laybutt, of Metallothioneins on beta cell function and survival as well as the emerging evidence revealing D.R.; Jonas, J.-C. Emerging Roles of negative effects and discuss the possible underlying mechanisms. We expose in parallel findings Metallothioneins in Beta Cell from other medical research fields and underscore unsettled questions. -
A Comparative Genomic Study in Schizophrenic and in Bipolar Disorder Patients, Based on Microarray Expression Pro�Ling Meta�Analysis
Hindawi Publishing Corporation e Scienti�c �orld �ournal Volume 2013, Article ID 685917, 14 pages http://dx.doi.org/10.1155/2013/685917 Research Article A Comparative Genomic Study in Schizophrenic and in Bipolar Disorder Patients, Based on Microarray Expression Pro�ling Meta�Analysis Marianthi Logotheti,1, 2, 3 Olga Papadodima,2 Nikolaos Venizelos,1 Aristotelis Chatziioannou,2 and Fragiskos Kolisis3 1 Neuropsychiatric Research Laboratory, Department of Clinical Medicine, Örebro University, 701 82 Örebro, Sweden 2 Metabolic Engineering and Bioinformatics Program, Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece 3 Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, 15780 Athens, Greece Correspondence should be addressed to Aristotelis Chatziioannou; [email protected] and Fragiskos Kolisis; [email protected] Received 2 November 2012; Accepted 27 November 2012 Academic Editors: N. S. T. Hirata, M. A. Kon, and K. Najarian Copyright © 2013 Marianthi Logotheti et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Schizophrenia affecting almost 1 and bipolar disorder affecting almost 3 –5 of the global population constitute two severe mental disorders. e catecholaminergic and the serotonergic pathways have been proved to play an important role in the development of schizophrenia, bipolar% disorder, and other related psychiatric% disorders.% e aim of the study was to perform and interpret the results of a comparative genomic pro�ling study in schizophrenic patients as well as in healthy controls and in patients with bipolar disorder and try to relate and integrate our results with an aberrant amino acid transport through cell membranes. -
The Roles of Metallothioneins in Carcinogenesis Manfei Si and Jinghe Lang*
Si and Lang Journal of Hematology & Oncology (2018) 11:107 https://doi.org/10.1186/s13045-018-0645-x REVIEW Open Access The roles of metallothioneins in carcinogenesis Manfei Si and Jinghe Lang* Abstract Metallothioneins (MTs) are small cysteine-rich proteins that play important roles in metal homeostasis and protection against heavy metal toxicity, DNA damage, and oxidative stress. In humans, MTs have four main isoforms (MT1, MT2, MT3, and MT4) that are encoded by genes located on chromosome 16q13. MT1 comprises eight known functional (sub)isoforms (MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1M, and MT1X). Emerging evidence shows that MTs play a pivotal role in tumor formation, progression, and drug resistance. However, the expression of MTs is not universal in all human tumors and may depend on the type and differentiation status of tumors, as well as other environmental stimuli or gene mutations. More importantly, the differential expression of particular MT isoforms can be utilized for tumor diagnosis and therapy. This review summarizes the recent knowledge on the functions and mechanisms of MTs in carcinogenesis and describes the differential expression and regulation of MT isoforms in various malignant tumors. The roles of MTs in tumor growth, differentiation, angiogenesis, metastasis, microenvironment remodeling, immune escape, and drug resistance are also discussed. Finally, this review highlights the potential of MTs as biomarkers for cancer diagnosis and prognosis and introduces some current applications of targeting MT isoforms in cancer therapy. The knowledge on the MTs may provide new insights for treating cancer and bring hope for the elimination of cancer. Keywords: Metallothionein, Metal homeostasis, Cancer, Carcinogenesis, Biomarker Background [6–10]. -
The Functions of Metamorphic Metallothioneins in Zinc and Copper Metabolism
International Journal of Molecular Sciences Review The Functions of Metamorphic Metallothioneins in Zinc and Copper Metabolism Artur Kr˛ezel˙ 1 and Wolfgang Maret 2,* 1 Department of Chemical Biology, Faculty of Biotechnology, University of Wrocław, Joliot-Curie 14a, 50-383 Wrocław, Poland; [email protected] 2 Division of Diabetes and Nutritional Sciences, Department of Biochemistry, Faculty of Life Sciences and Medicine, King’s College London, 150 Stamford Street, London SE1 9NH, UK * Correspondence: [email protected]; Tel.: +44-020-7848-4264; Fax: +44-020-7848-4195 Academic Editor: Eva Freisinger Received: 25 April 2017; Accepted: 3 June 2017; Published: 9 June 2017 Abstract: Recent discoveries in zinc biology provide a new platform for discussing the primary physiological functions of mammalian metallothioneins (MTs) and their exquisite zinc-dependent regulation. It is now understood that the control of cellular zinc homeostasis includes buffering of Zn2+ ions at picomolar concentrations, extensive subcellular re-distribution of Zn2+, the loading of exocytotic vesicles with zinc species, and the control of Zn2+ ion signalling. In parallel, characteristic features of human MTs became known: their graded affinities for Zn2+ and the redox activity of their thiolate coordination environments. Unlike the single species that structural models of mammalian MTs describe with a set of seven divalent or eight to twelve monovalent metal ions, MTs are metamorphic. In vivo, they exist as many species differing in redox state and load with different metal ions. The functions of mammalian MTs should no longer be considered elusive or enigmatic because it is now evident that the reactivity and coordination dynamics of MTs with Zn2+ and Cu+ match the biological requirements for controlling—binding and delivering—these cellular metal ions, thus completing a 60-year search for their functions. -
ID AKI Vs Control Fold Change P Value Symbol Entrez Gene Name *In
ID AKI vs control P value Symbol Entrez Gene Name *In case of multiple probesets per gene, one with the highest fold change was selected. Fold Change 208083_s_at 7.88 0.000932 ITGB6 integrin, beta 6 202376_at 6.12 0.000518 SERPINA3 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 1553575_at 5.62 0.0033 MT-ND6 NADH dehydrogenase, subunit 6 (complex I) 212768_s_at 5.50 0.000896 OLFM4 olfactomedin 4 206157_at 5.26 0.00177 PTX3 pentraxin 3, long 212531_at 4.26 0.00405 LCN2 lipocalin 2 215646_s_at 4.13 0.00408 VCAN versican 202018_s_at 4.12 0.0318 LTF lactotransferrin 203021_at 4.05 0.0129 SLPI secretory leukocyte peptidase inhibitor 222486_s_at 4.03 0.000329 ADAMTS1 ADAM metallopeptidase with thrombospondin type 1 motif, 1 1552439_s_at 3.82 0.000714 MEGF11 multiple EGF-like-domains 11 210602_s_at 3.74 0.000408 CDH6 cadherin 6, type 2, K-cadherin (fetal kidney) 229947_at 3.62 0.00843 PI15 peptidase inhibitor 15 204006_s_at 3.39 0.00241 FCGR3A Fc fragment of IgG, low affinity IIIa, receptor (CD16a) 202238_s_at 3.29 0.00492 NNMT nicotinamide N-methyltransferase 202917_s_at 3.20 0.00369 S100A8 S100 calcium binding protein A8 215223_s_at 3.17 0.000516 SOD2 superoxide dismutase 2, mitochondrial 204627_s_at 3.04 0.00619 ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) 223217_s_at 2.99 0.00397 NFKBIZ nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta 231067_s_at 2.97 0.00681 AKAP12 A kinase (PRKA) anchor protein 12 224917_at 2.94 0.00256 VMP1/ mir-21likely ortholog -
Heterogeneity Between Primary Colon Carcinoma and Paired Lymphatic and Hepatic Metastases
MOLECULAR MEDICINE REPORTS 6: 1057-1068, 2012 Heterogeneity between primary colon carcinoma and paired lymphatic and hepatic metastases HUANRONG LAN1, KETAO JIN2,3, BOJIAN XIE4, NA HAN5, BINBIN CUI2, FEILIN CAO2 and LISONG TENG3 Departments of 1Gynecology and Obstetrics, and 2Surgical Oncology, Taizhou Hospital, Wenzhou Medical College, Linhai, Zhejiang; 3Department of Surgical Oncology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang; 4Department of Surgical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang; 5Cancer Chemotherapy Center, Zhejiang Cancer Hospital, Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang, P.R. China Received January 26, 2012; Accepted May 8, 2012 DOI: 10.3892/mmr.2012.1051 Abstract. Heterogeneity is one of the recognized characteris- Introduction tics of human tumors, and occurs on multiple levels in a wide range of tumors. A number of studies have focused on the Intratumor heterogeneity is one of the recognized charac- heterogeneity found in primary tumors and related metastases teristics of human tumors, which occurs on multiple levels, with the consideration that the evaluation of metastatic rather including genetic, protein and macroscopic, in a wide range than primary sites could be of clinical relevance. Numerous of tumors, including breast, colorectal cancer (CRC), non- studies have demonstrated particularly high rates of hetero- small cell lung cancer (NSCLC), prostate, ovarian, pancreatic, geneity between primary colorectal tumors and their paired gastric, brain and renal clear cell carcinoma (1). Over the past lymphatic and hepatic metastases. It has also been proposed decade, a number of studies have focused on the heterogeneity that the heterogeneity between primary colon carcinomas and found in primary tumors and related metastases with the their paired lymphatic and hepatic metastases may result in consideration that the evaluation of metastatic rather than different responses to anticancer therapies. -
Research Article a Comparative Genomic Study in Schizophrenic and in Bipolar Disorder Patients, Based on Microarray Expression Pro�Ling Meta�Analysis
Hindawi Publishing Corporation e Scienti�c �orld �ournal Volume 2013, Article ID 685917, 14 pages http://dx.doi.org/10.1155/2013/685917 Research Article A Comparative Genomic Study in Schizophrenic and in Bipolar Disorder Patients, Based on Microarray Expression Pro�ling Meta�Analysis Marianthi Logotheti,1, 2, 3 Olga Papadodima,2 Nikolaos Venizelos,1 Aristotelis Chatziioannou,2 and Fragiskos Kolisis3 1 Neuropsychiatric Research Laboratory, Department of Clinical Medicine, Örebro University, 701 82 Örebro, Sweden 2 Metabolic Engineering and Bioinformatics Program, Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece 3 Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, 15780 Athens, Greece Correspondence should be addressed to Aristotelis Chatziioannou; [email protected] and Fragiskos Kolisis; [email protected] Received 2 November 2012; Accepted 27 November 2012 Academic Editors: N. S. T. Hirata, M. A. Kon, and K. Najarian Copyright © 2013 Marianthi Logotheti et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Schizophrenia affecting almost 1 and bipolar disorder affecting almost 3 –5 of the global population constitute two severe mental disorders. e catecholaminergic and the serotonergic pathways have been proved to play an important role in the development of schizophrenia, bipolar% disorder, and other related psychiatric% disorders.% e aim of the study was to perform and interpret the results of a comparative genomic pro�ling study in schizophrenic patients as well as in healthy controls and in patients with bipolar disorder and try to relate and integrate our results with an aberrant amino acid transport through cell membranes. -
MAFB Determines Human Macrophage Anti-Inflammatory
MAFB Determines Human Macrophage Anti-Inflammatory Polarization: Relevance for the Pathogenic Mechanisms Operating in Multicentric Carpotarsal Osteolysis This information is current as of October 4, 2021. Víctor D. Cuevas, Laura Anta, Rafael Samaniego, Emmanuel Orta-Zavalza, Juan Vladimir de la Rosa, Geneviève Baujat, Ángeles Domínguez-Soto, Paloma Sánchez-Mateos, María M. Escribese, Antonio Castrillo, Valérie Cormier-Daire, Miguel A. Vega and Ángel L. Corbí Downloaded from J Immunol 2017; 198:2070-2081; Prepublished online 16 January 2017; doi: 10.4049/jimmunol.1601667 http://www.jimmunol.org/content/198/5/2070 http://www.jimmunol.org/ Supplementary http://www.jimmunol.org/content/suppl/2017/01/15/jimmunol.160166 Material 7.DCSupplemental References This article cites 69 articles, 22 of which you can access for free at: http://www.jimmunol.org/content/198/5/2070.full#ref-list-1 by guest on October 4, 2021 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2017 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. -
Metallothioneins in Failure of Dental Implants and Periodontitis Down Syndrome Patients
G C A T T A C G G C A T genes Article Metallothioneins in Failure of Dental Implants and Periodontitis Down Syndrome Patients Maria Baus-Domínguez 1, Raquel Gómez-Díaz 2, Jose-Ramón Corcuera-Flores 3, Daniel Torres-Lagares 1,* , José-Cruz Ruiz-Villandiego 4, Guillermo Machuca-Portillo 3, José-Luis Gutiérrez-Pérez 1,5,* and María-Angeles Serrera-Figallo 3 1 Oral Surgery Department, Dentistry Faculty, University of Seville, 41009 Seville, Spain; [email protected] 2 Instituto de Biomedicina de Sevilla, 41007 Seville, Spain; [email protected] 3 Dentistry in Handicapped Patients Department, Dentistry Faculty, University of Seville, 41009 Seville, Spain; [email protected] (J.-R.C.-F.); [email protected] (G.M.-P.); [email protected] (M.-A.S.-F.) 4 Dentistry in Handicapped Patients Department, Quirón Hospital, 20012 San Sebastián, Spain; [email protected] 5 Oral and Maxillofacial Unit, Virgen del Rocio Hospital, 41009 Seville, Spain * Correspondence: [email protected] (D.T.-L.); [email protected] (J.-L.G.-P.) Received: 4 August 2019; Accepted: 12 September 2019; Published: 14 September 2019 Abstract: Background: Sometimes dental implants seem to be the only therapeutic alternative for the oral rehabilitation of patients with Down syndrome, given that they usually lose all their teeth early due to suffering aggressive periodontitis and they do not usually have the skills required to wear removable prostheses. However, the evolution of dental implants in these patients shows very adverse results. It is possible that basal genetic alterations, or at least some characteristics of these, may underlie these clinical results. The metabolic pathway of metallothioneins, molecules with an important influence on bone metabolism, could be one of the said alterations. -
Loss of 13Q Is Associated with Genes Involved in Cell Cycle and Proliferation in Dedifferentiated Hepatocellular Carcinoma
Modern Pathology (2008) 21, 1479–1489 & 2008 USCAP, Inc All rights reserved 0893-3952/08 $30.00 www.modernpathology.org Loss of 13q is associated with genes involved in cell cycle and proliferation in dedifferentiated hepatocellular carcinoma Britta Skawran1, Doris Steinemann1, Thomas Becker2, Reena Buurman1, Jakobus Flik3, Birgitt Wiese4, Peer Flemming5, Hans Kreipe5, Brigitte Schlegelberger1 and Ludwig Wilkens1,5 1Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany; 2Department of Visceral and Transplantation Surgery, Hannover Medical School, Hannover, Germany; 3Institute of Virology, Hannover Medical School, Hannover, Germany; 4Institute of Biometry, Hannover Medical School, Hannover, Germany and 5Institute of Pathology, Hannover Medical School, Hannover, Germany Dedifferentiation of hepatocellular carcinoma implies aggressive clinical behavior and is associated with an increasing number of genomic alterations, eg deletion of 13q. Genes directly or indirectly deregulated due to these genomic alterations are mainly unknown. Therefore this study compares array comparative genomic hybridization and whole genome gene expression data of 23 well, moderately, or poorly dedifferentiated hepatocellular carcinoma, using unsupervised hierarchical clustering. Dedifferentiated carcinoma clearly branched off from well and moderately differentiated carcinoma (Po0.001 v2-test). Within the dedifferentiated group, 827 genes were upregulated and 33 genes were downregulated. Significance analysis of microarrays for hepatocellular carcinoma with and without deletion of 13q did not display deregulation of any gene located in the deleted region. However, 531 significantly upregulated genes were identified in these cases. A total of 6 genes (BIC, CPNE1, RBPMS, RFC4, RPSA, TOP2A) were among the 20 most significantly upregulated genes both in dedifferentiated carcinoma and in carcinoma with loss of 13q. -
On the Sensitivity of Feature Ranked Lists for Large-Scale Biological Data
MATHEMATICAL BIOSCIENCES doi:10.3934/mbe.2013.10.667 AND ENGINEERING Volume 10, Number 3, June 2013 pp. 667{690 ON THE SENSITIVITY OF FEATURE RANKED LISTS FOR LARGE-SCALE BIOLOGICAL DATA Danuta Gawe land Krzysztof Fujarewicz Silesian University of Technology, Institute of Automatic Control Akademicka 16, 44-100 Gliwice, Poland Abstract. The problem of feature selection for large-scale genomic data, for example from DNA microarray experiments, is one of the fundamental and well-investigated problems in modern computational biology. From the com- putational point of view, a selected gene list should be characterized by good predictive power and should be understood and well explained from the biolog- ical point of view. Recently, another feature of selected gene lists is increasingly investigated, namely their stability which measures how the content and/or the gene order change when the data are perturbed. In this paper we propose a new approach to analysis of gene list stability, termed the sensitivity index, that does not require any data perturbation and allows the gene list that is most reliable in a biological sense to be chosen. 1. Introduction. Present techniques in molecular biology such as DNA microar- rays, mass spectrometry, and deep sequencing deliver vast data sets. A common property of these data sets is that the number of features (genes, peptides, etc.) is much greater than the number of samples (observations). This requires careful feature selection as the very first step of supervised data analysis. Many methods of gene selection have been proposed in the literature, which may be divided into two groups: univariate methods and multivariate methods.