PRODUCT INFORMATION Kanamycin a Item No

PRODUCT INFORMATION Kanamycin A Item No. 16140

CAS Registry No.: 59-01-8 Formal Name: O-3-amino-3-deoxy-α-D- OH OH

glucopyranosyl-(1→6)-O- OH OH H O O [6-amino-6-deoxy-α-D- NH2

glucopyranosyl-(1→4)]-2-deoxy-

O O D-streptamine OH H2N NH2 OH MF: C18H36N4O11 FW: 484.5

Purity: ≥95% H2N OH Supplied as: A crystalline solid Storage: -20°C Stability: As supplied, 2 years from the QC date provided on the Certificate of Analysis, when stored properly

Laboratory Procedures

Kanamycin A is supplied as a crystalline solid. Kanamycin A is sparingly soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. For biological experiments, we suggest that organic solvent-free aqueous solutions of kanamycin A be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of kanamycin A in PBS, pH 7.2, is approximately 5 mg/ml. We do not recommend storing the aqueous solution for more than one day.

Description

Kanamycin A is a broad spectrum antibiotic isolated from a soil streptomyces (S. kanamyceticus). This aminoglycoside alters translation by prokaryotic ribosomes, resulting in bacterial cell death.1 In molecular biology, kanamycin A is used as a selective agent to isolate transformants that are expressing a kanamycin resistance gene, usually neomycin phosphotransferase (NptII/neo). This mode of selection is most commonly used in plant and mammalian biology.

Reference

1. Misumi, M. and Tanaka, N. Mechanism of inhibition of translocation by kanamycin and viomycin: A comparative study with fusidic acid. Biochem. Biophys. Res. Commun. 92(2), 647-654 (1980).

WARNING CAYMAN CHEMICAL THIS PRODUCT IS FOR RESEARCH ONLY - NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE. 1180 EAST ELLSWORTH RD SAFETY DATA ANN ARBOR, MI 48108 · USA This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution. PHONE: [800] 364-9897

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