Cucurbit Genetics Cooperative (CGC) Was Organized in 1977 to Develop and Advance the Genetics of Economically Important Cucurbits
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CGC Coordinating Committee Chair: Timothy J Ng College Park, MD, USA Cucurbit Cucumber: Jack E. Staub Madison, WI, USA Melon: David W. Wolff Lehigh Acres, FL, USA Watermelon: Dennis T. Ray Genetics Tucson, AZ, USA Cucurbita spp.: Linda Wessel-Beaver Mayagúez, PR, USA Other genera: Mark G. Hutton Cooperative Monmouth, ME, USA CGC Gene List Committee Cucumber: Todd C. Wehner Raleigh, NC, USA Melon: Michel Pitrat Montfavet, FRANCE Watermelon: Stephen R. King College Station, TX Cucurbita spp.: R.W. Robinson Geneva, NY, USA Rebecca Brown 27 Corvallis, Oregon Harry S. Paris Ramat Yishay, ISRAEL Other genera: R.W. Robinson Geneva, NY, USA CGC Gene Curators Cucumber: Todd C. Wehner Raleigh, NC, USA Jack E. Staub 2004 Madison, WI, USA Melon: Michel Pitrat Montfavet, FRANCE James D. McCreight ISSN 1064-5594 Salinas, CA, USA Watermelon: Todd C. Wehner Raleigh, NC, USA 2118 Plant Sciences Building Xingping Zhang College Park, Maryland Woodland, CA, USA Cucurbita spp.: R.W. Robinson 20742-4452 USA Geneva, NY, USA Other genera: R.W. Robinson Tel: (301) 405-4345 Geneva, NY, USA Deena Decker-Walters Fax: (301) 314-9308 Miami, FL, USA Internet: [email protected] http://www.umresearch.umd.edu/cgc/ The Cucurbit Genetics Cooperative (CGC) was organized in 1977 to develop and advance the genetics of economically important cucurbits. Membership to CGC is voluntary and open to individuals who have an interest in cucurbit genetics and breeding. CGC membership is on a biennial basis. For more information on CGC and its membership rates, visit our website (http://www.umresearch.umd.edu/cgc/) or contact Tim Ng at (301) 405-4345 or [email protected]. CGC Reports are issued on an annual basis. The Reports include articles submitted by CGC members for the use of CGC members. None of the information in the annual report may be used in publications without the consent of the respective authors for a period of five years. ISSN 1064-5594 NEWS & COMMENT 25 Protocol for DNA Extraction from Watermelon leaves for SSR Marker v Comments from the CGC Coordinating Studies Committee v Comments from CGC Gene List G. Gusmini, T.C. Wehner, T. Joobeur, Committee R.A. Dean and A. Levi (USA) v Comments from the CGC Gene Curators v 2003 CGC Business Meeting 30 A Fasciated Mutant in Watermelon vi Cucurbitaceae 2006 vii 24th Annual Watermelon Research and N. Guner and T.C. Wehner (USA) Development Working Group Report viii The Cucurbit Network ix Upcoming Cucurbit Meetings 32 Accessions Having Opposite Leaf Arrangement at the First True Leaf in RESEARCH REPORTS Watermelon N. Guner and T.C. Wehner (USA) Cucumber (Cucumis sativus) 34 Lycopene and Total Carotenoid 1 Harvest-Dependent Chemical Content as Descriptors for Citrullus Components in Cucumis sativus L. lanatus: Limitations and Preliminary Fruits: I. Salad Cucumbers Trials G. Pevicharova and N. Velkov (Bulgaria) A.R. Davis, S.R. King, R.L. Jarret, A. Levi (USA) and Y. Tadmor (Israel) 5 Harvest-Dependent Chemical Components in Cucumis sativus L. 41 Cultivars Suitable for Watermelon Fruits: II. Pickling Cucumbers Rind Pickles G. Pevicharova and N. Velkov (Bulgaria) G. Gusmini and T.C. Wehner (USA) Melon (Cucumis melo) 43 Heterosis for Yield in a Watermelon Hybrid 8 Notes on the Change of the Causal G. Gusmini and T.C. Wehner (USA) Species of the Cucurbit Powdery Mildew in the U.S. 45 Estimates of Variance Components and J.D. McCreight (USA) Broad-Sense Heritability for Yield in Watermelon Watermelon (Citrullus lanatus) G. Gusmini and T.C. Wehner (USA) 24 Qualitative Genes for Use in Development of Elite Watermelon Cultivars T.C. Wehner and N. Guner (USA) iii 49 Some Considerations on Speed of 69 Habitat Studies for the Wild Stocks of Weighing Watermelon Fruit in Yield Ecballium elaterium (L.) A. Rich Trials E. Attard, A. Scicluna-Spiteri, and H. G. Gusmini, J.R. Schultheis and T.C. Attard (Malta) Wehner (USA) 73 The Cultivation and Cucurbitacin 51 In Search of High Lycopene Content of Ecballium elaterium (L.) A. Watermelon Rich P. Perkins-Veazie and A.R. Davis (USA) E. Attard and A. Scicluna-Spiteri (Malta) Cucurbita spp. GENE LISTS 54 Morphological Traits of Possible Use as 77 2004 Gene List for Cucurbita species Species Markers in Cucurbita moschata and C. argryosperma H.S. Paris, and R.N. Brown L. Wessel-Beaver, J.S. Gonzales, and H.C. Marrero (Puerto Rico) 97 Gene Nomenclature for the Cucurbitaceae 57 Enhanced Haploids Regeneration in Anther Culture of Summer Squash 98 Covenant and By-Laws of the Cucurbit (Curcurbita pepo L.) Genetics Cooperative M.F. Mohamed and E.F.S. Refaei (Egypt) 101 Membership Directory 61 Development and Characterization of Microsatellite Markers (SSR) in Cucurbita species G. Stift, A. Zraidi, and T. Lelley (Austria) Other Genera and Species 66 Variability Analysis of Underutilized Nutritive Vegetable Kartoli: Indian Collection D. Ram, S. Kumar, A. Verma and M. Rai (Naria) iv Comments: forward samples of currently held gene stocks to the respective Curator. Comments from the CGC Coordinating x Cucumber: Todd C. Wehner and Jack E. Staub Committee - 2004: The Call for Papers for the 2005 x Melon: Michael Pitrat and James D. Report (CGC Report No. 27) will be mailed in McCreight August 2004. Papers should be submitted to the x Watermelon: Todd C. Wehner and Stephen R. respective Coordinating Committee members by 31 King December 2004. The Report will be published by x Cucurbita spp.: Harry Paris and Richard W. June/July 2005. As always, we are eager to hear Robinson from CGC members regarding our current activities x Other Genera: Mark Hutton and Deena and the future direction of CGC. Decker-Walters x David Wolff (melon) x Todd C. Wehner (watermelon) 2003 CGC Business Meeting (Providence, x Mark G. Hutton (other genera) Rhode Island) x Jack E. Staub (cucumber) Tim Ng, CGC Chair Linda Wessel-Beaver (Cucurbita spp.) x The 2003 Business Meeting of the Cucurbit x Timothy Ng, Chair Genetics Cooperative was held on Friday, 3 Comments from CGC Gene List Committee: October 2003, in the Rhode Island Convention Lists of known genes for the Cucurbitaceae have Center in Providence, Rhode Island. Eighteen been published previously in Hortscience and in CGC members and guests were in attendance. reports of the Cucurbit Genetics Cooperative. CGC The meeting began with introductions around is currently publishing complete lists of known the room, then Tim Ng reported that CGC genes for cucumber (Cucumis sativus), melon (Cucumis melo), watermelon (Citrullus lanatus), and Report No. 25 (2002) had been printed and Cucurbita spp. on a rotating basis. mailed, albeit almost a year late. As an apology for the delay, Tim had paid for the printing and It is hoped that scientists will consult these lists as mailing himself, not using any CGC funds, well as the rules of gene nomenclature for the essentially giving every CGC member a free Cucurbitaceae before selecting a gene name and year of membership. He said that CGC Report symbol. Thus, inadvertent duplication of gene names and symbols will be prevented. The rules of gene No. 26 (2003) was being prepared, but that he nomenclature were adopted in order to provide was losing staff at the University of Maryland guidelines for the naming and symbolizing of genes due to budget cuts, plus the university Print previously reported and those which will be reported Shop he had used since 1988 was being closed. in the future. Scientists are urged to contact He did not yet have a target date for the printing members of the Gene List Committee regarding and mailing of CGC 26. Tim then described questions in interpreting the nomenclature rules and some updates to the CGC website, including a in naming and symbolizing new genes. javascript web-based application for x Cucumber: Todd C. Wehner and Jack E. Staub membership applications. x Melon: Michael Pitrat and James D. McCreight Upcoming cucurbit-related meetings were then x Watermelon: Todd C. Wehner and Stephen R. discussed. The 8th EUCARPIA Meeting on King Cucurbit Genetics and Breeding, x Cucurbita spp.: Harry Paris and Richard W. “Cucurbitaceae 2004,” was scheduled for 12-17 Robinson July 2004 at Palacký University in Olomouc, x Other Genera: Mark Hutton and Deena Czech Republic, with Aleš Lebeda as one of the Decker-Walters organizers. The webpage for the conference Comments from the CGC Gene Curators: CGC was already up, with links for deadlines, has appointed curators for the four major cultivated registration, and manuscript guidelines. Todd groups: cucumber, melon, watermelon and Wehner (North Carolina State University) Cucurbita spp. announced that Cucurbitaceae 2006 would be Curators are responsible for collecting, maintaining held in Asheville, North Carolina, during the and distributing upon request stocks of the known Fall of 2006, and that he would have more marker genes. CGC members are requested to details at the next CGC Business Meeting. Tim v mentioned that there were still some copies of With regard to membership dues, Tim indicated the proceedings from the Cucurbitaceae 2002 that he thought they could remain constant for conference in Naples, Florida, available through the next few years if there were no extraordinary the American Society for Horticultural Science increases in printing or postage costs. Tim also (ASHS), and that although no copies appeared mentioned that he was looking at the available at the ASHS website, they were amazon.com website the other day, and that the actually holding a few in reserve for present and CGC Report was listed there as a “Magazine future CGC members. Tim also mentioned that Subscription” for $28.85 an issue, which is he had recently received a number of copies of more than twice what CGC charges. Amazon the proceedings from the Cucurbitaceae 1994 listed this subscription as being provided by conference in Padre Island, Texas, and that “Magazine Express, Inc.” A similar entry was these copies would be made available to CGC listed for The Cucurbit Network (TCN) members.