TAXON 58 (4) • November 2009: 1153–1167 Rohwer & al. • Is Persea monophyletic? Is Persea (Lauraceae) monophyletic? Evidence from nuclear ribosomal ITS sequences Jens G. Rohwer1, Jie Li2, Barbara Rudolph1, Sabrina A. Schmidt1, Henk van der Werff 3 & Hsi-wen Li2 1 Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, 22609 Hamburg, Germany.
[email protected] (author for correspondence) 2 Laboratory of Plant Phylogenetics and Conservation Biology, Xishuangbanna Tropical Botanical Garden, The Chinese Academy of Sciences, 88 Xuefu Rd., Kunming, Yunnan 650223, P.R. China 3 Missouri Botanical Garden, P.O.Box 299, St. Louis, Missouri 63166-0299, U.S.A. The delimitation of genera within the Persea group (Lauraceae) has always been controversial. In an attempt to resolve the phylogenetic lines within this group, we analyzed ITS sequences of 61 species of the Persea group (Lauraceae) and 30 other species of Lauraceae. Several of the traditional genera or subgenera form well-supported groups, viz., Persea subg. Eriodaphne, Machilus, Persea subg. Persea, and Alseodaphne including Dehaasia. The included species of Phoebe form two clades that are unresolved with respect to Alseodaphne. However, Persea subg. Eriodaphne (together with the Macaronesian Apollonias barbujana) forms one of the clades of an unresolved basal trichotomy within the Persea group, whereas Persea subg. Persea is well supported as member of an oth- erwise Asian clade including Alseodaphne and Phoebe. This indicates that Persea, as currently circumscribed, is not monophyletic. The affinities of the Macaronesian Persea indica are clearly American rather than Asian. KEYWORDS: ITS, Lauraceae, neotropics, paleotropics, Persea group, phylogeny group. The species in this group have paniculate-cymose INTRODUCTION inflorescences, in which the lateral flowers of a cyme are The Persea group, with about 400 to 450 species, strictly opposite.