Identification of Porcine RUNX1 As an LPS-Dependent Gene Expression Regulator
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Prospective Isolation of NKX2-1–Expressing Human Lung Progenitors Derived from Pluripotent Stem Cells
The Journal of Clinical Investigation RESEARCH ARTICLE Prospective isolation of NKX2-1–expressing human lung progenitors derived from pluripotent stem cells Finn Hawkins,1,2 Philipp Kramer,3 Anjali Jacob,1,2 Ian Driver,4 Dylan C. Thomas,1 Katherine B. McCauley,1,2 Nicholas Skvir,1 Ana M. Crane,3 Anita A. Kurmann,1,5 Anthony N. Hollenberg,5 Sinead Nguyen,1 Brandon G. Wong,6 Ahmad S. Khalil,6,7 Sarah X.L. Huang,3,8 Susan Guttentag,9 Jason R. Rock,4 John M. Shannon,10 Brian R. Davis,3 and Darrell N. Kotton1,2 2 1Center for Regenerative Medicine, and The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA. 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center, Houston, Texas, USA. 4Department of Anatomy, UCSF, San Francisco, California, USA. 5Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA. 6Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, Massachusetts, USA. 7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA. 8Columbia Center for Translational Immunology & Columbia Center for Human Development, Columbia University Medical Center, New York, New York, USA. 9Department of Pediatrics, Monroe Carell Jr. Children’s Hospital, Vanderbilt University, Nashville, Tennessee, USA. 10Division of Pulmonary Biology, Cincinnati Children’s Hospital, Cincinnati, Ohio, USA. It has been postulated that during human fetal development, all cells of the lung epithelium derive from embryonic, endodermal, NK2 homeobox 1–expressing (NKX2-1+) precursor cells. -
Supplemental Materials ZNF281 Enhances Cardiac Reprogramming
Supplemental Materials ZNF281 enhances cardiac reprogramming by modulating cardiac and inflammatory gene expression Huanyu Zhou, Maria Gabriela Morales, Hisayuki Hashimoto, Matthew E. Dickson, Kunhua Song, Wenduo Ye, Min S. Kim, Hanspeter Niederstrasser, Zhaoning Wang, Beibei Chen, Bruce A. Posner, Rhonda Bassel-Duby and Eric N. Olson Supplemental Table 1; related to Figure 1. Supplemental Table 2; related to Figure 1. Supplemental Table 3; related to the “quantitative mRNA measurement” in Materials and Methods section. Supplemental Table 4; related to the “ChIP-seq, gene ontology and pathway analysis” and “RNA-seq” and gene ontology analysis” in Materials and Methods section. Supplemental Figure S1; related to Figure 1. Supplemental Figure S2; related to Figure 2. Supplemental Figure S3; related to Figure 3. Supplemental Figure S4; related to Figure 4. Supplemental Figure S5; related to Figure 6. Supplemental Table S1. Genes included in human retroviral ORF cDNA library. Gene Gene Gene Gene Gene Gene Gene Gene Symbol Symbol Symbol Symbol Symbol Symbol Symbol Symbol AATF BMP8A CEBPE CTNNB1 ESR2 GDF3 HOXA5 IL17D ADIPOQ BRPF1 CEBPG CUX1 ESRRA GDF6 HOXA6 IL17F ADNP BRPF3 CERS1 CX3CL1 ETS1 GIN1 HOXA7 IL18 AEBP1 BUD31 CERS2 CXCL10 ETS2 GLIS3 HOXB1 IL19 AFF4 C17ORF77 CERS4 CXCL11 ETV3 GMEB1 HOXB13 IL1A AHR C1QTNF4 CFL2 CXCL12 ETV7 GPBP1 HOXB5 IL1B AIMP1 C21ORF66 CHIA CXCL13 FAM3B GPER HOXB6 IL1F3 ALS2CR8 CBFA2T2 CIR1 CXCL14 FAM3D GPI HOXB7 IL1F5 ALX1 CBFA2T3 CITED1 CXCL16 FASLG GREM1 HOXB9 IL1F6 ARGFX CBFB CITED2 CXCL3 FBLN1 GREM2 HOXC4 IL1F7 -
E Proteins Sharpen Neurogenesis by Modulating Proneural Bhlh
RESEARCH ARTICLE E proteins sharpen neurogenesis by modulating proneural bHLH transcription factors’ activity in an E-box-dependent manner Gwenvael Le Dre´ au1†*, Rene´ Escalona1†‡, Raquel Fueyo2, Antonio Herrera1, Juan D Martı´nez1, Susana Usieto1, Anghara Menendez3, Sebastian Pons3, Marian A Martinez-Balbas2, Elisa Marti1 1Department of Developmental Biology, Instituto de Biologı´a Molecular de Barcelona, Barcelona, Spain; 2Department of Molecular Genomics, Instituto de Biologı´a Molecular de Barcelona, Barcelona, Spain; 3Department of Cell Biology, Instituto de Biologı´a Molecular de Barcelona, Barcelona, Spain Abstract Class II HLH proteins heterodimerize with class I HLH/E proteins to regulate transcription. Here, we show that E proteins sharpen neurogenesis by adjusting the neurogenic strength of the distinct proneural proteins. We find that inhibiting BMP signaling or its target ID2 in *For correspondence: the chick embryo spinal cord, impairs the neuronal production from progenitors expressing [email protected] ATOH1/ASCL1, but less severely that from progenitors expressing NEUROG1/2/PTF1a. We show this context-dependent response to result from the differential modulation of proneural proteins’ †These authors contributed activity by E proteins. E proteins synergize with proneural proteins when acting on CAGSTG motifs, equally to this work thereby facilitating the activity of ASCL1/ATOH1 which preferentially bind to such motifs. Present address: Conversely, E proteins restrict the neurogenic strength of NEUROG1/2 by directly inhibiting their ‡ Departamento de Embriologı´a, preferential binding to CADATG motifs. Since we find this mechanism to be conserved in Facultad de Medicina, corticogenesis, we propose this differential co-operation of E proteins with proneural proteins as a Universidad Nacional Auto´noma novel though general feature of their mechanism of action. -
Transcription Factor ATF5 Is Required for Terminal Differentiation and Survival of Olfactory Sensory Neurons
Transcription factor ATF5 is required for terminal differentiation and survival of olfactory sensory neurons Shu-Zong Wanga,b, Jianhong Oub, Lihua J. Zhub,c, and Michael R. Greena,b,1 aHoward Hughes Medical Institute, bPrograms in Gene Function and Expression and Molecular Medicine, and cProgram in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA 01605 Edited by Solomon H. Snyder, The Johns Hopkins University School of Medicine, Baltimore, MD, and approved September 27, 2012 (received for review June 21, 2012) Activating transcription factor 5 (ATF5) is a member of the ATF/cAMP not been determined. Here we address this issue by deriving and −/− response element-binding family of transcription factors, which characterizing Atf5 mice. compose a large group of basic region leucine zipper proteins whose members mediate diverse transcriptional regulatory functions. Results −/− ATF5 has a well-established prosurvival activity and has been found Derivation of Atf5 Mice. To investigate the physiological role of −/− to be overexpressed in several human cancers, in particular glioblas- ATF5, we derived Atf5 mice. The gene-targeting strategy in- toma. However, the role(s) of ATF5 in development and normal volved replacing almost the entire coding region of Atf5 with a physiology are unknown. Here we address this issue by deriving LacZ-Neo cassette, such that the LacZ gene is under the control and characterizing homozygous Atf5 knockout mice. We find that of the endogenous Atf5 promoter (Fig. S1A). The genomic struc- +/+ +/− −/− Atf5−/− pups die neonatally, which, as explained below, is consis- ture of the Atf5 locus in Atf5 , Atf5 ,andAtf5 mice was fi tent with an olfactory defect resulting in a competitive suckling con rmed by Southern blot (Fig. -
Molecular Regulation in Dopaminergic Neuron Development
International Journal of Molecular Sciences Review Molecular Regulation in Dopaminergic Neuron Development. Cues to Unveil Molecular Pathogenesis and Pharmacological Targets of Neurodegeneration Floriana Volpicelli 1 , Carla Perrone-Capano 1,2 , Gian Carlo Bellenchi 2,3, Luca Colucci-D’Amato 4,* and Umberto di Porzio 2 1 Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy; fl[email protected] (F.V.); [email protected] (C.P.C.) 2 Institute of Genetics and Biophysics “Adriano Buzzati Traverso”, CNR, 80131 Rome, Italy; [email protected] (G.C.B.); [email protected] (U.d.P.) 3 Department of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, Italy 4 Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy * Correspondence: [email protected]; Tel.: +39-0823-274577 Received: 28 April 2020; Accepted: 1 June 2020; Published: 3 June 2020 Abstract: The relatively few dopaminergic neurons in the mammalian brain are mostly located in the midbrain and regulate many important neural functions, including motor integration, cognition, emotive behaviors and reward. Therefore, alteration of their function or degeneration leads to severe neurological and neuropsychiatric diseases. Unraveling the mechanisms of midbrain dopaminergic (mDA) phenotype induction and maturation and elucidating the role of the gene network involved in the development and maintenance of these neurons is of pivotal importance to rescue or substitute these cells in order to restore dopaminergic functions. Recently, in addition to morphogens and transcription factors, microRNAs have been identified as critical players to confer mDA identity. The elucidation of the gene network involved in mDA neuron development and function will be crucial to identify early changes of mDA neurons that occur in pre-symptomatic pathological conditions, such as Parkinson’s disease. -
Notch Controls Multiple Pancreatic Cell Fate Regulators Through Direct Hes1-Mediated Repression
bioRxiv preprint doi: https://doi.org/10.1101/336305; this version posted June 1, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Notch Controls Multiple Pancreatic Cell Fate Regulators Through Direct Hes1-mediated Repression by Kristian H. de Lichtenberg1, Philip A. Seymour1, Mette C. Jørgensen1, Yung-Hae Kim1, Anne Grapin-Botton1, Mark A. Magnuson2, Nikolina Nakic3,4, Jorge Ferrer3, Palle Serup1* 1 Novo Nordisk Foundation Center for Stem Cell Biology (Danstem), University of Copenhagen, Denmark. 2 Center for Stem Cell Biology, Vanderbilt University, Tennessee, USA. 3 Section on Epigenetics and Disease, Imperial College London, UK. 4 Current Address: GSK, Stevenage, UK. *Corresponding Author: [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/336305; this version posted June 1, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Notch signaling and its effector Hes1 regulate multiple cell fate choices in the developing pancreas, but few direct target genes are known. Here we use transcriptome analyses combined with chromatin immunoprecipitation with next-generation sequencing (ChIP-seq) to identify direct target genes of Hes1. ChIP-seq analysis of endogenous Hes1 in 266-6 cells, a model of multipotent pancreatic progenitor cells, revealed high-confidence peaks associated with 354 genes. Among these were genes important for tip/trunk segregation such as Ptf1a and Nkx6-1, genes involved in endocrine differentiation such as Insm1 and Dll4, and genes encoding non-pancreatic basic-Helic-Loop-Helix (bHLH) factors such as Neurog2 and Ascl1. -
Blocking Notch-Signaling Increases Neurogenesis in the Striatum After Stroke
cells Communication Blocking Notch-Signaling Increases Neurogenesis in the Striatum after Stroke 1 1, 2 2 Giuseppe Santopolo , Jens P. Magnusson y, Olle Lindvall , Zaal Kokaia and Jonas Frisén 1,* 1 Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden; [email protected] (G.S.); [email protected] (J.P.M.) 2 Lund Stem Cell Center, University Hospital, SE-221 84 Lund, Sweden; [email protected] (O.L.); [email protected] (Z.K.) * Correspondence: [email protected] Current affiliation: Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. y Received: 13 June 2020; Accepted: 16 July 2020; Published: 20 July 2020 Abstract: Stroke triggers neurogenesis in the striatum in mice, with new neurons deriving in part from the nearby subventricular zone and in part from parenchymal astrocytes. The initiation of neurogenesis by astrocytes within the striatum is triggered by reduced Notch-signaling, and blocking this signaling pathway by deletion of the gene encoding the obligate Notch coactivator Rbpj is sufficient to activate neurogenesis by striatal astrocytes in the absence of an injury. Here we report that blocking Notch-signaling in stroke increases the neurogenic response to stroke 3.5-fold in mice. Deletion of Rbpj results in the recruitment of a larger number of parenchymal astrocytes to neurogenesis and over larger areas of the striatum. These data suggest inhibition of Notch-signaling as a potential translational strategy to promote neuronal regeneration after stroke. Keywords: neurogenesis; astrocyte; stem cell; stroke; striatum 1. Introduction Every year, 13.7 million people worldwide suffer a stroke, of whom 5.5 million die and as many suffer permanent loss of function with debilitating outcomes [1]. -
1-S2.0-S0959438816302392-Main
Beyond proneural: emerging functions and regulations of proneural proteins François Guillemot, Bassem A. Hassan To cite this version: François Guillemot, Bassem A. Hassan. Beyond proneural: emerging functions and regula- tions of proneural proteins. Current Opinion in Neurobiology, Elsevier, 2017, 42, pp.93 - 101. 10.1016/j.conb.2016.11.011. hal-01434997 HAL Id: hal-01434997 https://hal.sorbonne-universite.fr/hal-01434997 Submitted on 13 Jan 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives| 4.0 International License Available online at www.sciencedirect.com ScienceDirect Beyond proneural: emerging functions and regulations of proneural proteins 1 2 Franc¸ois Guillemot and Bassem A Hassan Proneural proteins, which include Ascl1, Atoh1 and in multipotent cells and a role in fate specification distin- Neurogenins e´ pinie` re in vertebrates and Achaete-Scute guish proneural proteins from neuronal differentiation proteins and Atonal in Drosophila, are expressed in the factors such as members of the mammalian Neurod developing nervous system throughout the animal kingdom family, which are first expressed in cells already commit- and have an essential and well-characterised role in specifying ted to a neuronal fate and promote their differentiation the neural identity of progenitors. -
Primary Cilia Regulate Hippocampal Neurogenesis by Mediating Sonic Hedgehog Signaling
Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling Joshua J. Breunig*†, Matthew R. Sarkisian*†, Jon I. Arellano*, Yury M. Morozov*, Albert E. Ayoub*, Sonal Sojitra‡, Baolin Wang§¶, Richard A. Flavellʈ**, Pasko Rakic*††, and Terrence Town‡ʈ *Department of Neurobiology and Kavli Institute of Neuroscience and §Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, W404, New York, NY 10021; ¶Department of Cell and Developmental Biology, Weill Medical College of Cornell University, 1300 York Avenue, W404, New York, NY 10021; ʈDepartment of Immunobiology and **Howard Hughes Medical Institute, Yale University School of Medicine, 300 Cedar Street, TAC S-569, New Haven, CT 06519-8011; and ‡Departments of Biomedical Science, Neurosurgery, and Medicine, Maxine Dunitz Neurosurgical Institute, Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles, CA 90048 Contributed by Pasko Rakic, May 10, 2008 (sent for review March 20, 2008) Primary cilia are present on mammalian neurons and glia, but their tivity, we noted that the typical lamination and morphology of function is largely unknown. We generated conditional homozy- glial cells was severely disrupted (Fig. 1 E–H). Glial cell bodies, gous mutant mice for a gene we termed Stumpy. Mutants lack cilia as shown by combined Sox2 and Gfap immunoreactivity, were and have conspicuous abnormalities in postnatally developing located more randomly in NestinCre; Stumpyfl/fl mice (hereto- brain regions, including a hypoplasic hippocampus characterized fore referred to as ⌬Stumpy mice), and there was a notable lack by a primary deficiency in neural stem cells known as astrocyte-like of DG radial glia (Fig. 1 A, B, and E–H). -
Highly Dynamic Chromatin Interactions Drive Neurogenesis Through Gene
bioRxiv preprint doi: https://doi.org/10.1101/303842; this version posted April 18, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Highly dynamic chromatin interactions drive neurogenesis through gene regulatory networks Valeriya Malysheva1,2,4, Marco Antonio Mendoza-Parra1, Matthias Blum1,3 and Hinrich Gronemeyer1,4 1 Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Equipe Labellisée Ligue Contre le Cancer, Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, University of Strasbourg, Illkirch, France. 2 Present address: The Babraham Institute, Babraham Research Campus, Cambridge, UK 3 Present address: European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, UK 4 Corresponding authors: Valeriya Malysheva Email: [email protected] Phone: +(44) 1223 49 6505 Hinrich Gronemeyer Email: [email protected] Phone: +(33) 3 88 65 34 73 bioRxiv preprint doi: https://doi.org/10.1101/303842; this version posted April 18, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Cell fate transitions are fundamental processes in the ontogeny of multicellular organisms1 and aberrations can generate pathologies2. Cell fate acquisition is a highly complex phenomenon which involves a plethora of intrinsic and extrinsic instructive signals that direct the lineage progression of pluripotent cells. -
The Hormone-Bound Vitamin D Receptor Regulates Turnover of Target
The hormone-bound vitamin D receptor regulates turnover of target proteins of the SCFFBW7 E3 ligase By Reyhaneh Salehi Tabar Department of Experimental Medicine McGill University Montreal, QC, Canada April 2016 A thesis submitted to McGill University in partial fulfillment of the requirements of the degree of Doctor of Philosophy © Reyhaneh Salehi Tabar 1 Table of Contents Abbreviations ................................................................................................................................................ 7 Abstract ....................................................................................................................................................... 10 Rèsumè ....................................................................................................................................................... 13 Acknowledgements ..................................................................................................................................... 16 Preface ........................................................................................................................................................ 17 Contribution of authors .............................................................................................................................. 18 Chapter 1-Literature review........................................................................................................................ 20 1.1. General introduction and overview of thesis ............................................................................ -
Subtype Heterogeneity and Epigenetic Convergence in Neuroendocrine Prostate Cancer
bioRxiv preprint doi: https://doi.org/10.1101/2020.09.13.291328; this version posted September 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Subtype Heterogeneity and Epigenetic Convergence in Neuroendocrine Prostate Cancer Paloma Cejas1,2,3, Yingtian Xie1,2, Alba Font-Tello1,2, Klothilda Lim1,2, Sudeepa Syamala1,2, Xintao Qiu1,2, Alok K. Tewari1,2,4, Neel Shah1,2, Holly M Nguyen5, Radhika A. Patel6, Lisha Brown5, Ilsa Coleman6, Wenzel M. Hackeng7, Lodewijk Brosens7, Koen M.A. Dreijerink8, Leigh Ellis4,9, Sarah Abou Alaiwi1, Ji-Heui Seo1, Mark Pomerantz1, Alessandra Dall'Agnese10, Jett Crowdis1,4, Eliezer M. Van Allen1,4, Joaquim Bellmunt11, Colm Morrisey5, Peter S. Nelson6, James DeCaprio1, Anna Farago12, Nicholas Dyson12, Benjamin Drapkin13,14,15, X. Shirley Liu2,16, Matthew Freedman1,2, Michael C. Haffner6,17,18, Eva Corey5, Myles Brown1,2* and Henry W. Long1,2* 1. Department of Medical Oncology, Dana-Farber Cancer Institute, Brigham and Women’s Hospital, and Harvard Medical School, Boston, Massachusetts, USA. 2. Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. 3. Translational Oncology Laboratory, Hospital La Paz Institute for Health Research (IdiPAZ) and CIBERONC, La Paz University Hospital, Madrid, Spain. 4. Broad Institute of MIT and Harvard, Cambridge, MA, USA 5. Department of Urology, University of Washington, Seattle, WA, USA 6. Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA, USA 7.