Family Tree Dna Complaints
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Genetic Testing and the Implications for Future Therapies Genetic Testing for Inherited Marrow Failure
4/24/2017 Personalized Medicine: What This Talk Will Cover Genetic Testing and the Implications for Future Therapies Genetic testing for inherited marrow failure . General background: What are gene mutations? . Risk and severity: Do all people with inherited mutations develop disease? National Patient and Living with . How does medical genetic testing differ from commercially available testing? Aplastic Anemia, Family Conference MDS or PNH Las Vegas, NV April 22, 2017 Cytogenetic testing in Bone Marrow Failure Genomic profiling of bone marrow cells in MDS and AA for acquired mutations Implications for diagnosis, prognosis and therapies Katherine R. Calvo, M.D., Ph.D. Department of Laboratory Medicine, Clinical Center National Institutes of Health Benefits and Limitations of Genetic Testing and Genomic Profiling Bethesda, Maryland Bone marrow failure syndromes: How can genetic testing help to distinguish and guide therapy? AA/PNH Part One: PNH LGL Autoimmune SDS Disease: AA MS, IBD, uveitis, HYPOCELLULAR Genetic Testing for DM type 1, etc. TELOMERE MDS (DKC) GATA2 MDS AML Inherited Marrow Failure FA Acquired AA SDS AA, aplastic anaemia; AID, autoimmune disease; AML, acute myelogenous leukemia; DKC, dyskeratosis congenita; IBD, inflammatory bowel disease; LGL, large granular lymphocyte leukemia; GATA2, Gata2 deficiency; FA, Fanconi anemia; SDS, Shwachman–Diamond syndrome; MDS, myelodysplastic syndrome; MS, multiple sclerosis; DM, diabetes mellitus; PNH, paroxysmal nocturnal hemoglobinuria Modified from Young NS, et al. Blood 2006;108:2509–19 -
The Polymerase Chain Reaction (PCR): the Second Generation of DNA Analysis Methods Takes the Stand, 9 Santa Clara High Tech
Santa Clara High Technology Law Journal Volume 9 | Issue 1 Article 8 January 1993 The olP ymerase Chain Reaction (PCR): The Second Generation of DNA Analysis Methods Takes the Stand Kamrin T. MacKnight Follow this and additional works at: http://digitalcommons.law.scu.edu/chtlj Part of the Law Commons Recommended Citation Kamrin T. MacKnight, The Polymerase Chain Reaction (PCR): The Second Generation of DNA Analysis Methods Takes the Stand, 9 Santa Clara High Tech. L.J. 287 (1993). Available at: http://digitalcommons.law.scu.edu/chtlj/vol9/iss1/8 This Comment is brought to you for free and open access by the Journals at Santa Clara Law Digital Commons. It has been accepted for inclusion in Santa Clara High Technology Law Journal by an authorized administrator of Santa Clara Law Digital Commons. For more information, please contact [email protected]. THE POLYMERASE CHAIN REACTION (PCR): THE SECOND GENERATION OF DNA ANALYSIS METHODS TAKES THE STAND Kamrin T. MacKnightt TABLE OF CONTENTS INTRODUCTION ........................................... 288 BASIC GENETICS AND DNA REPLICATION ................. 289 FORENSIC DNA ANALYSIS ................................ 292 Direct Sequencing ....................................... 293 Restriction FragmentLength Polymorphism (RFLP) ...... 294 Introduction .......................................... 294 Technology ........................................... 296 Polymerase Chain Reaction (PCR) ....................... 300 H istory ............................................... 300 Technology .......................................... -
Codis Dna Database
INDIANAPOLIS-MARION COUNTY FORENSIC SERVICES AGENCY Doctor Dennis J. Nicholas Institute of Forensic Science 40 SOUTH ALABAMA STREET INDIANAPOLIS, INDIANA 46204 PHONE (317) 327-3670 FAX (317) 327-3607 Michael Medler Laboratory Director Evidence Submission Guideline #7 CODIS DNA DATABASE The Combined DNA Index System (CODIS) is a computer database of DNA profiles used to solve crimes across the United States. In Indiana, DNA profiles obtained from the following categories can be entered into CODIS by the Indianapolis Marion County Forensic Services Agency: crime scene evidence, missing persons and unidentified human remains. The purpose of the database is to generate investigative leads. Not all cases are considered CODIS eligible. CODIS is maintained at the national level by the Federal Bureau of Investigation which has requirements to determine the eligibility of a DNA profile for CODIS entry. A continued “life of crime” or the high incidence of recidivism in criminals is the concept upon which the DNA database program is based. If a convicted offender’s profile matches crime scene evidence, this can identify a potential suspect(s). The database can also link multiple unsolved crimes to identify serial offenses and connect agencies who can then work together in the investigation. Finally, CODIS can be used to identify human remains by matching them to missing persons or to their biological relatives. To maximize the value of this program, biological evidence from unsolved crimes and missing persons cases must be analyzed and the DNA profile developed is entered into CODIS. I. NO SUSPECT/UNSOLVED CASES A. All unsolved cases with potential probative DNA qualifying case evidence, including those with no suspects, should be submitted to the Indianapolis-Marion County Forensic Services Agency (IMCFSA) for analysis. -
The Police National DNA Database: Balancing Crime Detection, Human Rights and Privacy
The Police National DNA Database: Balancing Crime Detection, Human Rights and Privacy A Report by GeneWatch UK The Police National DNA Database: Balancing Crime Detection, Human Rights and Privacy. A Report for GeneWatch UK by Kristina Staley January 2005 The Mill House, Manchester Road, Tideswell, Buxton Derbyshire, SK17 8LN, UK GeneWatch Phone: 01287 871898 Fax: 01298 872531 E-mail: [email protected] UK Website: www.genewatch.org Acknowledgements GeneWatch would like to thank Jan van Aken, Sarah Sexton and Paul Johnson for their helpful comments on a draft of this report. Kristina Staley would also like to thank Val Sales for her help in preparing the report. The content of the final report remains the responsibility of GeneWatch UK. Cover photograph DNA genetic fingerprinting on fingerprint blue backdrop. © Adam Hart-Davis, http://www.adam-hart-davis.org/ GeneWatch UK 2 January 2005 Contents 1 Executive summary ................................................................................................................5 2 Introduction...........................................................................................................................10 3 What is the National DNA Database?..................................................................................11 3.1 Using DNA to identify individuals...................................................................................11 3.2 How the police use DNA................................................................................................12 3.3 Concerns -
The Canada's History Beginner's Guide to Genetic
THE CANADA’S HISTORY BEGINNER’S GUIDE TO GENETIC GENEALOGY Read in sequence or browse as you see fit by clicking on any navigation item below. Introduction C. How to proceed A. To test or not Testing strategies for beginners Reasons for testing Recovery guide for those who tested and were underwhelmed Bogus reasons for not testing Fear of the test D. Case studies “The tests are crap” Confirming a hypothesis with autosomal DNA Price Refuting a hypothesis with autosomal DNA Substantive reasons for not testing Confirming a hypothesis with Y DNA Privacy concerns Developing (and then confirming) a hypothesis with Unexpected findings autosomal DNA Developing a completely unexpected hypothesis from B. The ABCs of DNA testing autosomal DNA The four major testing companies (and others) Four types of DNA and three major genetic genealogy tests E. Assorted observations on interpreting DNA tests Mitochondrial DNA (mtDNA) Y DNA F. More resources Autosomal DNA (atDNA) Selected recent publications X DNA Basic information about genetic genealogy Summarizing the tests Blogs by notable genetic genealogists (a selective list) Tools and utilities © 2019 Paul Jones The text of this guide is protected by Canadian copyright law and published here with permission of the author. Unless otherwise noted, copyright of every image resides with the image’s owner. You should not use any of these images for any purpose without the owner’s express authorization unless this is already granted in a cited license. For further information or to report errors or omissions, please contact Paul Jones. CANADASHISTORY.CA ONLINE SPECIAL FEATURE 2019 1 Introduction The “bestest best boy in the land” recently had his DNA tested. -
So Many Ancestors, So Little Time
GENEALOGY WEBSITES National/International Subscription Sites (fee charged for these four sites): Ancestry - This is a subscription site (genealogy.com is a member site): http://www.ancestry.com Ancestry Library Edition - Often available through public libraries, as well as the Minnesota Historical Society: http://www.ancestrylibrary.com Geneanet – Search volunteer submissions. Has a variety of records available for free; premium access by subscription: http://en.geneanet.info/ ProGenealogists - A division of Ancestry; hire a professional to do the research for you: http://www.progenealogists.com/ World Vital Records - Has limited records available for free, detailed records by subscription; ancestry.com has many of the same records: http://worldvitalrecords.com/ FREE sites (Available at no cost as of March 2015; some require registration.) Family Search - This site has many of the same features as Ancestry.com, including many census records: http://familysearch.org Rootsweb - User donated family trees (includes blank forms and charts; requires free registration to post and respond to queries): http://www.rootsweb.ancestry.com/ or to search trees directly, go to: http://wc.rootsweb.com Find-A-Grave - GREAT site for finding burial information, dates, obituaries, etc.: http://www.findagrave.com Tombstone Transcription Project - Great for finding more genealogy links for cities, counties and countries: http://www.usgwtombstones.org/ The US Genweb Project – Sponsored by Rootsweb; includes links to state sites: http://usgenweb.org/ Statue of -
Is Our Tree Better Than My Tree? the Benefits and Pitfalls of Collaborative Genealogy by E
Is Our Tree Better Than My Tree? The Benefits and Pitfalls of Collaborative Genealogy by E. Randol Schoenberg The following article is based upon a presentation given at than 100,000 profiles. the IAJGS conference in Boston, August 2013—Ed. Merging Duplicates, Adding Sources, Finding Matches. ntil recently, we genealogists began by building our The better sites allow merging of duplicate profiles, so that U own individual family trees. We started with our par- if a person appears more than once in the tree, the profiles ents, siblings, children and spouses and continued to fill in may be joined together seamlessly. This avoids unnecessary as much as we could. When we reached a branch where we duplication of profiles in the tree. Sites such as Ances- did not know the information, we turned to a relative or try.com and MyHeritage also offer the ability to connect searched for records, perhaps even hired a professional ge- records from their enormous databases to the profiles in the nealogist. With time and patience, many of us built nice tree, which is a huge bonus for those who like to have their trees, even large ones, with hundreds or thousands of peo- trees well documented. Users also have the ability to search ple. We documented our results with records and photo- the unconnected trees of other users and contact them to graphs. confirm or trade information. The companies even have Most of us eventually computerized our trees, using pro- developed algorithms to detect data matches and suggest grams such as Family Tree Maker or Reunion. -
You Can't Take It with You!
You Can’t Take It With You! RGS CIG, 12 Sep 2013 & 8 Sep 2016 All Slides are available at… • www.dennisAhogan.com • Look for Lectures & Handouts tab • Everything is free on the site ©2014-2016, Dennis A. Hogan www.dennisAhogan.com What has changed in the last 3 years • Online trees are developing – Supporting infrastructure is improving – Sourcing is improving – Relevant apps are improving • Familysearch’s Memories allows uploading content and links to individuals in your family – Can upload text, images, audio – Free – UNLIMITED space ©2014-2016, Dennis A. Hogan www.dennisAhogan.com Online Trees • The big 4 all offer free trees – Familysearch, Ancestry, MyHeritage, FindMyPast • 3 other sites I’ll mention are – Wikitree, WeRelate, Geni • Some are free sites, some are subscription sites • Some are “One Tree” and some are not – “One Tree” means the goal is to have only 1 instance of each individual ©2014-2016, Dennis A. Hogan www.dennisAhogan.com Online Trees $ site Import Type tree type autosearch free familysearch.org ~no gedcom one tree Y free wikitree.com gedcom one tree N free werelate.org gedcom one tree N pay ancestry.com gedcom Y pay myheritage.com gedcom Y pay geni.com no gedcom one tree Y pay findmypast gedcom Y On Pay sites: can search, but to see all content must subscribe On Pay sites: non-subscribers can not contact you On familysearch: anyone can modify info in “your” tree, but they can not modify anything in your Memories ©2014-2016, Dennis A. Hogan www.dennisAhogan.com In the Good Ol’ Days… • We only had physical stuff – stuff that everyone could see & touch – Books – Binders – Folders – Maps – Photos – Boxes – Paper scraps – Sticky notes – Ephemera & other artifacts ©2014-2016, Dennis A. -
Diving Deeper Into Genetic Genealogy Handout
DIVING DEEPER INTO GENETIC GENEALOGY Presented by Melissa A. Johnson, CGSM [email protected] ETHNICITY RESULTS testing has not been particularly useful for The ethnicity information (also known as genealogy, but tests are now more refined biogeographical data) that is part of a test and this is changing. taker’s overall DNA test results is based • STR (short tandem repeat) testing examines on comparisons of the test taker’s DNA to a specific number of STR markers (as chosen sample populations. Each of the DNA testing by the test taker—typically 12, 37, 67, or 111 companies uses different calculations to markers). Tests of 37 markers or more can compare the test taker’s DNA to proprietary be genealogically useful. sample populations, as well as to publicly available sample population data. As a result, With Y-chromosome STR testing, a test taker’s a test taker’s ethnicity information will vary Y-DNA matches are determined by the number from company to company. The science behind of identical STR marker values. Identifying ethnicity results is still in its infancy. Ethnicity how closely related a test taker is to his Y-DNA results can be interesting, but they are not as matches can be difficult, as a number of factors useful for genealogical research as examining need to be considered, including: DNA matches. • the number of non-matching STR marker values in relation to the number of markers tested; and Y-CHROMOSOME DNA TESTING • the mutation rates for the tested markers Y-chromosome DNA is passed down the male (some STR markers mutate at higher rates line, from father to son. -
National DNA Databases 2011
NATIONAL FORENSIC DNA DATABASES COUNCIL FOR RESPONSIBLE GENETICS ANDREW D. THIBEDEAU, J.D. SENIOR FELLOW National DNA Databases 2011 National Forensic DNA Databases Table of Contents Executive Summary .................................................................................................................. - 5 - Summary Table ..................................................................................................................... - 5 - Laws on Point ...................................................................................................................... - 10 - Entry Criteria ...................................................................................................................... - 13 - Introduction & Trends ........................................................................................................... - 15 - National Status Reports Part I - Operational DNA Databases ........................................... - 19 - Australia............................................................................................................................... - 20 - Austria .................................................................................................................................. - 24 - Bahrain................................................................................................................................. - 27 - Belarus ................................................................................................................................. - -
Genetic Genealogy Genetic Genealogy
Family History Research Using Genetic Genealogy Genetic Genealogy ● DNA Testing Companies ● Three most common types of testing using DNA ● Y-DNA ● mtDNA ● Autosomal DNA (atDNA) (including X-dna) ● DNA Analysis Tools Genetic Genealogy ● Main testing companies to choose from: ● Family Tree DNA - www.familytreedna.com ● Y-dna ($139USD), mtDNA ($79-199USD), atDNA ($79, includes ethnicity, not medically focussed) ● Accepts transfers from some other testing companies (possibly free, or ~$19USD) ● 23 and ME – www.23andme.com ● atDNA ($249CDN, includes ethnicity, medically focussed) ● Ancestry – www.dna.ancestry.com ● atDNA ($149CDN, includes ethnicity, not medically focussed) ● MyHeritage - www.myheritage.com ● atDNA $79USD, accepts dna transfers for free ● LivingDNA - www.livingdna.com ● atDNA $143CDN, plans to accept transfers in the near future ● *https://isogg.org/wiki/Autosomal_DNA_testing_comparison_chart (comparison details) Genetic Genealogy ● Three most common types of testing using DNA ● Y-DNA ● mtDNA ● Autosomal DNA (atDNA) (including X-dna) ● Each tests a different type of dna and they CANNOT be compared to each other ! Don’t compare apples to oranges! Genetic Genealogy ● Y-DNA for direct male line (test for men only) ● mtDNA for direct female line (test for men and women) Genetic Genealogy ● Y-DNA mtDNA mtDNA Inheritance male / female Genetic Genealogy ● Y-DNA genetic testing ● The y chromosome is only passed down from a man to his son. ● Every man has a y chromosome that has been passed down to him from thousands and thousands of generations of fathers to sons going back into the dawn of humanity (National Genographic Project). ● Since the start of the use of surnames fathers have tended to pass on their surname along with a y chromosome ● “Surname” projects have become very popular as people try to link together groups of men with a certain surname. -
Where Have All the Indians Gone? Native American Eastern Seaboard Dispersal, Genealogy and DNA in Relation to Sir Walter Raleigh’S Lost Colony of Roanoke
Where Have All the Indians Gone? Native American Eastern Seaboard Dispersal, Genealogy and DNA in Relation to Sir Walter Raleigh’s Lost Colony of Roanoke. Roberta Estes Copyright 2009, all rights reserved, submitted for publication [email protected] or [email protected] Abstract Within genealogy circles, family stories of Native American1 heritage exist in many families whose American ancestry is rooted in Colonial America and traverses Appalachia. The task of finding these ancestors either genealogically or using genetic genealogy is challenging. With the advent of DNA testing, surname and other special interest projects2, tools now exist to facilitate grouping participants in a way that allows one to view populations in historical fashions. This paper references and uses data from several of these public projects, but particularly the Melungeon, Lumbee, Waccamaw, North Carolina Roots and Lost Colony projects3. The Lumbee have long claimed descent from the Lost Colony via their oral history4. The Lumbee DNA Project shows significantly less Native American ancestry than would be expected with 96% European or African Y chromosomal DNA. The Melungeons, long held to be mixed European, African and Native show only one ancestral family with Native DNA5. Clearly more testing would be advantageous in all of these projects. This phenomenon is not limited to these groups, and has been reported by other researchers such as Bolnick (et al, 2006) where she reports finding in 16 Native American populations with northeast or southeast roots that 47% of the families who believe themselves to be full blooded or no less than 75% Native with no paternal European admixture find themselves carrying European or African y-line DNA.