ARTICLE 401 et C. , 1 I , Quentin 1 , and Michael 4 I > 4 Davidsoniella Davidsoniella B % Key words: Eucalyptus Poaceae 4 et al. &''+ ;<<= Teratosphaeria and Teratosphaeria ., including s.lat., including 1996, Scott 2009). These et al. 1996, Scott 2009). F. F. IMA FUNGUS · 9(2): 401–418 (2018) 401–418 · 9(2): FUNGUS IMA C. I $ G 1 . The , Lieschen De Vos 4 et al. &''+ ! ;<<* > > $ F 4 Fusarium pseudomolecules for the pseudomolecules O B 3 A 1 s.lat., including
et al. &''* 3 4 et al. 1986, Miles U $ I 4 @ $ 2006) ? $ G B > 4 , Kasia Dadej ! " # $ % &'*+ ! 1976, Miles $ conditions 3 4 al. " $ $ " 4 ! E E spartinae, spartinae, 4 Claviceps purpurea purpurea Claviceps cf. % A @ % A@ ? $ C. P F 2 " E ) is a plant % # 1 and > I ! K and C. 4,5 [ E G E Davidsoniella eucalypti, Quambalaria 4 . E Hypocreales Fusarium fujikuroi J 4 > K = spartinae D 3 E F 1 cf. , Nicole Soal 1 J 4 and C. , Miao Liu , C. humidiphila 4
> $ P T 4,5 Grosmannia galeiformis , C. humidiphila,
P G 4 4 E E %%G! $ &; }
Teratosphaeria destructans, destructans, and Teratosphaeria , C. humidiphila E 3 4 3 ! $ [ [ G ? $ I $ A {& > |*<; G 3 ! $ [ [ G ? $ I $ A {& > |*<; G # % 4 > @ > G !K \ ` &<+= &q; ;< 3 A 4 > A4> U \ ] 3 $ ! ^% ! '*< ! $ U U P& \ 3 $ ! ^% ! &<& \ &<< > > \*> & _ ! destructans Article info: G x ;& F $ ;<&+v x ;* F $ ;<&+v I x &q 3 ;<&+. circinatum ". circinatum arundinis of , draft genome pathogen Fusarium circinatum pitch canker eucalypti, galeiformis, Quambalaria eucalypti, Grosmannia circinatum eucalypti insect associate J B > D $ arundinis Abstract: I q !K \ I $ A ";< D I <<;+ G v ^ x A B z% A@ K © 2018 International Mycological Association © 2018 International Mycological conditions: distribute and transmit the work, under the following are free to share - to copy, You Attribution: Non-commercial: No derivative works: if you get Any of the above conditions can be waived For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Nothing in this license impairs or restricts the author’s moral rights. permission from the copyright holder. 5 = 4 1 2 A 3 B C. Santana Nine draft genome sequences of sequences genome Nine draft IMA Genome-F 10 IMA B INTRODUCTION Claviceps purpurea (Clavicipitaceae, (Poaceae IMA Genome-F 10A IMA Nine draft genome sequences of Claviceps purpurea s.lat., including C. arundinis doi:10.5598/imafungus.2018.09.02.10 VOLUME 9 · NO. 2 cf. spartinae et al.
$ I K $ et al. 2000) in C. purpurea NUCLEOTIDE SEQUENCE ACCESSION s. lat E NUMBERS ^ C. arundinis, C. humidiphila, and C. spartinae E (Douhan et al. ;<<+ I $ et al. ;<&_ 33AJ[>A#4 A A I I\JF qq'=*& E
ARTICLE G $ $ [\3<&<<<<<< " ` # et al. 2016). In Canada, [B<&<<<<<< [{<&<<<<<< [ <&<<<<<< G [O<&<<<<<< [\ <&<<<<<< [\A<&<<<<<< > ;<<; > K ] [\!<&<<<<<< [\[<&<<<<<< \ E ;<&q 3 $ F!A@ G\ x $ ! $ G\I&='*&< x C. spartinae 4= !. humidiphila, and the C. purpurea MATERIALS AND METHODS s. lat. " % & D $ $ } % I 3 " I3 v A3 3 TM I $ &< at 20 °! > &M; $
} F2 3F " SEQUENCED STRAINS !E A 3 ] 3 &'+| E Claviceps purpurea s.str. $ \F " \F Canada: Saskatchewanx [ $ GP& q' &q F &<; '' B x & K Triticum aestivum, 2000, R. Clear |<< ) ;{ !E A &q< ) \F ! TM J 4 > K #>;+ 3 U>! ;_<*q| Czech Republic: [ K > " q" $ x ; _ ) _< ) A K D q' += F &q <= [ Secale cereale, v @ $ E % G ;+ ) ;<<= G I $ #>_+; 3 U>! ;_&|;= \F G[ q" $ x & ) &<< ) !!!||& "^ *M| ; &< ) I P _< ) )# v % Claviceps purpurea s.lat. G E % G Canada: Albertax F G _+ _= F &&+ &; B ; __ °! = A 3F Bromus inermis | G &'_* B I ! =_ \F ! #>|+ 3 U>! ;_<_|+ v > _; q& F &&< *= B $ " Elymus albicans | G &'_* B q_ M &
I ! #>+& 3 U>! ;_<_+& Quebecx ! F *_< 3# !D! 4 &= <<< >\! > I ^I _< &= F *& <+ B &_ I \F 3F Ammophila sp., 8 Sep. 2015, J. Cayouette ] Y. Dalpé & #>q_+ 3 U>! ;_&+'+ ;*<;+< F 3 &<<< G $= + E % G Claviceps cf. spartinae \F 3F } ® 2 .0 Canada: Manitobax 4 R % G q' <_ F '+ ** % @ $ # E B Phalaris arudinacea, 2014, J. Menzies E " 3F K q<< > # #>;&+ 3 U>! ;_&+q= Quebec: =<< ! $ #[;;< > ^! I ^E $ E # G ^J q+ *| F |& +| B Ammophila I!\ # F"G } %\[[ # 3F # breviligulata =& ;<&q J. Cayouette > # # R #>q_q 3 U>! ;_&+q_ 3 U> __<;q* M @ " $ } F " G } @ v Claviceps humidiphila > E # U \ ] Germany: Bavariax I q+ +* F &= *+ [ 3 $ ! ^% ! Dactylis sp., 1998 and #>_|* ;"&_< F " G } > U \ P @ 3 U>! ;_&|&| !!!q=q "^ E % ! $< && _ x } Claviceps arundinis } I } $ E Czech Republicx D $ 3$ G ` q' <& $< =* A et al. 2014) x F &q q= [ Phragmites australis, 11 Jan. 2008, HEADCROP:20 SLIDINGWINDOW:4:20 MINLEN:36. The #>_+= 3 U>! ;_&|;q !!!'== "^ A D F et al. ;<&= . De novo
402 IMA FUNGUS IMA Genome – F10
( & ARTICLE !" !" %"# %"# $ ( % $ ! $ # ! $ # 99/100 ! $ # ! $ # 80/98 ! $ # ! $ # 100/100 60/85 " #
$ $ $ $ $ $ 97/100 $ $ $ $ $ $ $ $ ! $ $ &$! %& &$! %& % #$ )& !&* # )& !&* # % & # # # 86/100 # # 90/100 # # # # 68/92 ) ) $ # % &' ( "%# #
Fig. 1. U >I Claviceps [%&^ '' $ =<; !@ < *<* \@ < +<| \! < q+' D@ < ='q 4^ ^|_ <+' ` >I A@
GI $= &< & A $ et al. ;<&; &<<< ? GE $q _ 4 $ et ! al. ;<&= $ !
VOLUME 9 · NO. 2 403 et al.
A ; $; < < # ] G K ;<&; A ; G > $ A > G > $< & &' # et al. ;<<'
Complete (%) BUSCO’s $ $; ; 4 ^
ARTICLE et al. ;<&; E $ ? $ G ^! U A?G!U $; (Simão et al. ;<&_ (x) ' 4 4 > ^[G $q =+ # K et al. ;<<_ O P E ^D $ et al. ;<<+ $ 4 4 D et al. ;<&q ; x $ ; ; K E & E 3F } &^ " Geneious 10.0.9 P N50 (bp) L50 GC (%) Coverage et al. ;<&; v $ } $ I $ et al. (2015) and Van # et al. ;<&* > %%E $ | https:// $ ) P ] G ;<&=. =<&''_<'28571566 ;||===<*'q'&= 21589 =;|=_+|__'q 46550 51,7 416=<_'+;_< 41916 190 51,4=<*';_+q 72 =''+q 189 51,5=
a a a _& _ E F_< ;& * q* * E A?G!U '* ' '+ * v +q&< ';=< The genome of Claviceps purpurea ;< & F!A@ . ! 4 <<<<<<<< & $ G et al. ;<&= E &qq; ++;=
et al. ;<&= K =< ' > F_< q* _ 4! _& * D $ &'& } LM78 3 U>! ;_<_|+ LM458LM218 3 U>! ;_&+'+ LM454 3 U>! ;_&+q= LM576 3 U>! ;_&+q_ #>_+= 3 U>! ;_&|&| !!!q=q 3 U>! ;_&|;q !!!'== 20,1 LM28LM582 3 U>! ;_<*q| 3 U>! ;_&|;= !!!||& B A?G!U
'| * U } Claviceps Claviceps s.lat. s.lat . s.lat. LM81 3 U>! ;_<_+& s.str. s.str. $ $ spartinae spartinae
cf. cf. Authors: H.D.T. Nguyen, W. Findlay, K. Dadej, O. Pochopski, P. Shoukouhi, J.G. Menzies, * &' * + /3 6 &7 ;T> ; 6 ? @ ["^ x !!!||& "^ !!!q=q "^ !!!'== "^ E } G Claviceps purpurea Claviceps purpurea Claviceps purpurea Claviceps Claviceps Claviceps humidiphila Claviceps arundinis Claviceps purpurea Claviceps purpurea Claviceps purpurea Species Strain # Voucher # Version number of Table 1. Statistics of Table a b *Contact: Miao Liu, z
404 IMA FUNGUS IMA Genome – F10
IMA Genome-F 10B $ %%G! ARTICLE E $ $ Assembling pseudomolecules for F. circinatum the pitch canker pathogen, Fusarium %%G! % K circinatum, utilising additional } ^ genome sequence data and synteny } B " K within the Fusarium fujikuroi %%G! B et al. ;<&= complex De Vos et al. ;<&q $ @ INTRODUCTION complement of F. circinatum E $ ^ } Fusarium $ $ $ 4 et al. ;<&= > F. circinatum $ +< $ $ associated Fusarium # ] G ;<<* %%G! B Fusarium fujikuroi " %%G! Fusarium. _< $ UR3 et al. 1998, 2000). Fusarium circinatum ^ SEQUENCED STRAIN O P Pinus USA: Californiax > Pinus radiata), 1996, spp. and Pseudotsuga menziesii 3 % @ T.R. Gordon, A.J. Storer & D. Okamoto %GI=q >\!|+|< !>B_&|_; I\[> *;&'|M 4 et al. 2015). NUCLEOTIDE SEQUENCE ACCESSION E } NUMBER 4 A J`<<<<<<<< $ J`<&<<<<<< B et al. 2012). The isolate E $ Fusarium circinatum, } %GI=q 4 et al. 1996), has a genetic 33AJ[>A# $ 3 ` et al. ;<<| 4 A J`<<<<<<<< $ 3 ` et al. ;<&q J`<;<<<<<< K } $ E# } 3 ` et al. ;<&& ` B et al. 2018). E 'q + B et al. MATERIALS AND METHODS ;<&| " q&q_ B et al. 2012, De Vos et al. 2014) and this limits its Fusarium circinatum $ " ;< " $ E } ;_ ! &;< %%G! $ | 3F " E Fusarium verticillioides > et al &''; 3F } } && $ F 3 ( G (Ma et al. 2010). This F. verticillioides " } F. circinatum " %GI=q PG&| %%G! E q&q_ B et al. ^ ;<&; @ PG&| %%G! { et al. 1995, Ma et al. ;<&< B et al. ;<&= P. radiata B ! ` F et al. ;<&q @ G ;<<_ G et al. 2014). The genomes } $ of F. circinatum %GI=q PG&| } $ F. fujikuroi B et ^ & K al. ;<&= E GU# 3( `q A G } %%G! ! D @ +; q_ &_= '_ ^ them and F. temperatum $ $ $ I } } $ B et ;< =* al. ;<&= 3 ` et al. 2014). This highlights the fact that $ !#! 4 B $ _ & !#! $
VOLUME 9 · NO. 2 405 et al. ARTICLE
Fig. 2. > " } !^ &^ G et al. 2011, De Vos et al ;<&q G } > %%E $ | P ] G ;<&= E > E $ ; & | 4 ] 4 ;<<= 3 et al. ;<&; ^ E@>;@4 E $ &'+* " ># I ># $ = & 4 et al ;<&< ` $ 1 +_ E F. circinatum %GI=q bold.
GGI ![ $ ; < A K et al. ;<&& K RESULTS AND DISCUSSION ^ %GI=q 4 A J`<<<<<<<< B et al. 2012), using E $ Fusarum circinatum, ^ 3 33AJ [>A#4 A J`<<<<<<<< ;<< E $ $ $ J`<;<<<<<< E F. circinatum # B } _+_ $ " K q= '=; '&; 3F F_< =*= *== E E $ ;|= +;" 4! q| q& && } &^&& F. verticillioides Fusarium %%G! > et al. ;<&< B E et al. 2012, Jeong et al ;<&= B et al. ;<&= ` A# GE et al. 1990) against a local Nest et al. ;<&q ! et al ;<&_ B et al. 2015a, F. verticillioides genome (DDBJ/EMBL/ F et al ;<&| B et al ;<&| 4 4 A @><<<<<<<< ; !#! ;<&+ G $ $ et al. ;<&+ ` B et al ;<&+ B 4 B E &; et al. ;<&+ &q ';= &; F. ^ ==' *+ fujikuroi B et al. ;<&= F. temperatum B I et al. ;<&_ $ E } " $ F. circinatum (Fig. 2). &<< FR G I && F. verticillioides and F. fujikuroi F. verticillioides and the pseudomolecules of F. circinatum I &; >?> $ = ;; P K et al. 2004). $ &; F. fujikuroi B E > P[\ '* '| ca q; *< > ! et al. ;<<+ K 4 F. circinatum scaffolds to these 12 pseudomolecules. These [G E ^D $ et al. ;<<+ G ] '' <' &_ <*< > ;<<_ GF I P ;< F. circinatum B et al. 2012). B Genomic alignments of these 12 pseudomolecules to the et al. ;<&; $ F. F. verticillioides and F. fujikuroi verticillioides, Fusarium oxysporum f. sp. lycopersici and % = E ^ $ F. graminearum (Ma et al. ;<&< " $ Fusarium } [GE $ F. circinatum B et FFSC. al. ;<&;
406 IMA FUNGUS IMA Genome – F10 ARTICLE
A B
Fig. 3. B x A. Fusarium verticillioides F. circinatum pseudomolecules. B. F. fujikuroi to F. circinatum @ $
CONCLUSIONS IMA Genome-F 10C
\ } $ $ $ Fusarium circinatum K Draft genome sequence of A Quambalaria eucalypti K %%G! B et al ;<&= 3 ` et al ;<&q &; INTRODUCTION E F. circinatum $ The genus Quambalaria ( Quambalariaceae, Microstromatales, Exobasidiomycetes, Basidiomycotina } Myrtaceae (De $ A et al. 2006, Pegg et al. ;<<' E " of F. circinatum E $ } Q. coyrecup Corymbia $ calophylla B I et al. 2008), and Q. $ cyanescens %%G! @ P et al. " ;<&_ E } %%G! ^ E $ P et al. 2006). Of the leaf and shoot pathogens in $ the genus, Q. pitereka and Q. eucalypti Quambalaria pitereka affects Corymbia Authors: ? Y Z7[ \] *; 3; ; 6^ _Y (Pegg et al. 2009) and China (Zhou et al. ;<<+ Q. `{ *3 >;|} ; 6~' ]7 3 eucalypti causes disease on Eucalyptus G *Contact: z K B et al. &''= A K et al. 2001) ? (I K et al. 2008) (Pegg et al. 2008), I A et al. 2015). \ Quambalariaceae " 3 A et al. ;<<* P ;<&| I et al. 2008), (Pegg et al. ;<<' ;<&& D $ ^ Malasseziales 3 A et al. ;<<* B et al. ;<&_ %
VOLUME 9 · NO. 2 407 et al.
## ¨ ## ## ## Q. cyanescens
ARTICLE ## ## ¨ Q. simpsonii # Q. eucalypti # # ¨ # ## ## ## Q. pitereka ## ##
# # Q. coyrecup # #
0.005
Fig. 4 I ># \ "># @EG } } species of Quambalaria E 3F " E "^ v FE F E v FGB F G B v B B v #3 G $ &<<< $ $ |_
" Quambalariaceae $ SEQUENCED STRAIN " South Africa: KwaZulu Natalx P Eucalyptus \ ^ " grandis E 4&; > &'+| - /012[4 5 Ustilaginomycotina !>B&&<&!AG&&++qq "^ v I\[> _&<+' species in Microstromatales $ M $ Quambalariaceae P et al. ;<&+ B } $ " $ NUCLEOTIDE ACCESSION NUMBER et al. 2014), the Malasseziales { et al. ;<<| E } Quambalaria eucalypti Polyporales (James et al. ;<&= 4 A E I \\ !<<<<<<<< E $ } Q. eucalypti E } \\ !<&<<<<<<. " $ $ $ D } !>B&&<& MATERIALS AND METHODS I\[> _&<+' Q. eucalypti. 4 3F " > ; > " < _ " v ;
408 IMA FUNGUS IMA Genome – F10
A > G ARTICLE Duong et al. ;<&= E 3F > I et al. ;<<< ! et al. 2009, G P ^ _<< D et al. 2011) E $ T. destructans K } @ D } ;_<< @ &''* B et ;_< ^ &<< { $ al. 1996) E ` [ E } !#! E # ! G (Old et 4 B $ | _ & !#! al. ;<<= A et al. ;<<* A et al. ;<&; 4 } et al. 2016) @ de novo ^ ^ ^ Eucalyptus camaldulensis, E $ E. grandis and E. urophylla A ? $ G ^! U species B et al. 1996, Old et al. ;<<= A ;<Basidiomycota dataset E T. destructans $ (Simao et al. ;<&_ E $ $ = = ; G et al. 2008) using Eucalyptus et al. 2011). ^ Ustilago maydis. E $ T. destructans on clonal E. grandis × E. urophylla G RESULTS AND DISCUSSION et al. ;<&& 4 et al. 2016). E } $ =& G $ '** ;;_ _+= qq' of T. destructans $ Eucalyptus species $ K ;q =+q F_< $ $ R *; *<< E K ;= _ $ (Old et al. ;<<= @ > K 4! } G T. destructans is *< E K K . E $ } Exobasidiomycetes T. destructans $ &| > &' > " Tilletia caries $ $ $ " K ;' _ > P ;<&= G et al. 2014, Toome et al. G ;<&q B et al. ;<&_ P et al. 2018). BUSCO $ +q _ E $ $ &&;+ A?G!U &<'= ^ A?G!U =_ A?G!U &;' A?G!U |+ A?G!U SEQUENCED STRAIN &==_ A?G!U ?4?GE?G |;q& $ I South Africa: Kwa-Zulu Natalx P } } Eucalyptus grandis × E. urophylla, ;<&_ I. Greyling " Q. eucalypti (Fig. 4). E $ !>B qq'*; I\[> *;;<| M Q. eucalypti $ Quambalariaceae $ Exobasidiomycetes. NUCLEOTIDE ACCESSION NUMBER G $ $ $ The Teratosphaeria destructans B 4 G 33AJ[F 4 A Authors: S.W. Morgan, T. A. Duong, M. Coetzee, M.J. \@A <<<<<<<< E $ ; Y _ /* $ \@A <&<<<<<< *Contact: z K
MATERIALS AND METHODS IMA Genome-F 10D Teratosphaeria destructans !>B qq'*; Draft genome sequence of !>B % A @ % A@ the Eucalyptus pathogen ? $ I ^ Teratosphaeria destructans 4 et al. 2016) >[ " v A G v < = " v U" INTRODUCTION A ;_ ! K ^ 3F " The genus Teratosphaeria " =< K ^ _
VOLUME 9 · NO. 2 409 et al.
ARTICLE
Fig. 5. > " # Teratosphaeria } Teratosphaeria destructans E !^ [%&^ $ $ et al. ;<&q et al. 2018).
&= $ I`I G $ A ? $ Steinheim " % I G ^! U A?G!U $ ; < & %I&;< K ! ! G } (Simão et al. 2015) A ; $ & & ; *_< ) !E A " &<< > E ^D! D+v ;_ G > $ & _ $ > [3E v ; > F ! v = _ !E Av ; G3G ; ) _<< $ # G Steinheim q _ $$ (Li et al. ;<<' # ] G K ;<&; ? GE $ !^ _ < & > et al. ;<&+ *< ! ;< & _ genome statistics. $ x ;qx& E !^ &^ [%&^ " &_ +&<< g E ^ " & _ $ Teratosphaeria $ et al. 2014) " x ;qx& &_ the T. destructans } E } &* =<< g E $ } $ & _ > et al. ;<&q et al. 2018) F!A@ =< M;< ! & _ $ x $ =< ;q ! E O P I ;< &* =<< g to collect 3 et al. 2008, 2010) E 3F |< E included a MUSCLE alignment [ ;<# 4 ] 4 ;<<= E 3F ! $ ] 4 ;<<* . % ! % G ? $ G G } @ 4 G G_ F " ^4 G } RESULTS AND DISCUSSION @ _=< &; *<< R E =; =&* &;< E q&=; U &+=| _<< GI $ = &; < F et al. ;<&= ^$ =& *& > E 4! ;& == __ || '' &;| E _& += F_< $ &<= &&'
410 IMA FUNGUS IMA Genome – F10
='+ |_| K #_< $ ARTICLE 'q E $ $ Xyleborini, Corthylini and Xyloterini $ > &|<" E A?G!U et al. ;<&_ E " '_ +* ;|+ $ Chalaropsis $ A?G!U ;'< genus Huntiella E } Teratosphaeria destructans } 3 A et al. 2014). The genus Davidsoniella % _ ! D.eucalypti, D. et al. ;<&& 4 et neocaledoniae and D. australis al. ;<&* A ] B ;<&| $ D. virescens F 3 } $ E A et al. 2014). $ @ Teratosphaeria Davidsoniella eucalypti Eucalyptus D et al. 2011) G $ Eucalyptus P et al. 1996). $ K $ pathogenic (Kile et al. &''* @ D. virescens, D. of fungal species P et al. 2011, Condon et al. australis, and D. neocaledoniae ^ ;<&= O et al. ;<&= 3 et al. 2017) E $ Acer spp.), } $ Nothofagus cunninghamii P &''= Coffea $ ^ (Gnocato robusta 3 &'_< $ et al. ;<&| \ et al. 2018) Davidsoniella eucalypti and D. virescens $ $ $ " 3 A et al. ;<&q $ M Authors: F.A. Lane*, C.T. Tatham, J. Aylward, M. Havenga, D. virescens D. eucalypti D P.M. Wilken, T.A. Duong, M.A. van der Nest, et al. &''+ B } D. virescens ; _ Y B et al. ;<&_ *Contact % # z% A@ K D. eucalypti Davidsoniella } E $ IMA Genome-F 10E
Draft nuclear genome assembly for SEQUENCED STRAIN
Davidsoniella eucalypti Australia: Victoria: ! E ! Eucalyptus sieberi, &'+' M.J. Dudzinski !>B =;_q ! *=' 3 \ |<;<_ M INTRODUCTION
E Ceratocystidaceae NUCLEOTIDE SEQUENCE ACCESSION $ && NUMBER 3 A et al. ;<&q ;<&| > et al. 2015, Nel et al. ;<&+ E ^ Ceratocystis, a genus E B 4 G Davidsoniella eucalypti !>B =;_q 33AJ the mango and Acacia mangium pathogen C. manginecans [F 4 A \>AB<<<<<<<< E et al. ;<&= E et al. 2011) and the causal $ $ \>AB<&<<<<<< C. cacaofunesta A [ ] D ;<<_ G Endoconidiophora and Thielaviopsis MATERIALS AND METHODS $ > et al. ;<&q B et al. ;<&= E Bretziella and Berkeleyomyces Davidsoniella eucalypti !>B =;_q ^ % Br. fagacearum $ Ceratocystis fagacearumv 3 A @ % A@ ; " A et al. ;<&| ^ Be. basiola >[ x ; $ A G ;_ ! &q^ Thielaviopsis basicolav Nel et al. ;<&+ > 3F " Ceratocystidaceae $ ^ \ " et al. ;< G ` B et al. ;<&= @ P ^ } Ambrosiella, Meredithiella, and Phialophoropsis I A G G ^ G>\E
VOLUME 9 · NO. 2 411 et al.
I A } E } @ D G } ;_<< > % __< K ^ ;_< E 3F @ }
ARTICLE " 3 et al. ;<&= E G>\E } } ^ ! $& q P et al. ;<&| E GGI ![^# \ A K et al. ;<&& A K ] I $ ;<&q ^ } ! E ^ Fig. 6. " ^ @ !#! 4 Davidsoniella ecualypti \ B $&& < & G Davidsoniella Endoconidiophora E " " $ ^ AB # ] 3 ;<<' G > # et al. 2009). E I B et al. ;<&q $ ^ '* & E &=&_ &;=* ^ single copies, 27 as complete duplicated copies, 16 as E $ =* @ ^ E D. eucalypti 4 % A K ] I $ ;<&; ^ D. virescens % * B et al. ;<&_ == * > + = > E than that of D. eucalypti E A ? $ G ! U '<;' vs *'_= D. virescens), A?G!U $ ; < & G et al. 2015) and the Ascomycota ;<| ;&_ > D. virescens and D. eucalypti $ @ de novo K ^ ?4?GE?G Fusarium graminearum ^ $ P et al. ;<&& G et al. ;<<* $ 3F 4! " % K et al. F_< #_< K ;<&+ > ] G ;<&| \ ] P ;<&; ? GE $_ < & > et al. 2018) E K ^ D. E *# } Davidsoniella $ 4 ] 4 ;<<= A $ E " $ ] Gascuel 2006). K D. eucalypti $
RESULTS AND DISCUSSION Authors: P.M. Wilken*, N. Soal, K. Naidoo, T.A. Duong, ; _ Y E q& +|q _&_ Davidsoniella *Contact: > B z K eucalypti &;&' &<<< & <*_ +=* E 4! q_ '= $ $ *'" F_< $ ;=< <'; #_< $ _& ?4?GE?G '<;' A?G!U
412 IMA FUNGUS IMA Genome – F10
IMA Genome-F 10F ARTICLE
Draft genome sequence of Grosmannia galeiformis
INTRODUCTION
Grosmannia galeiformis Ceratocystis galeiformes G &'_& A &'_& @ Ophiostoma (as Ophiostoma galeiformev > ^P &'_= Grosmannia (as G. galeiformisv Zipfel et al. 2006). Grosmannia galeiformis ^ [ A &'_& # et al. ;<&; > ^ P &'_= O et al. ;< et al ;<&& 4 % SEQUENCED STRAIN $ & &< A K ] I $ ;<&; E A United Kingdom: Elgin: on Pinus sylvestris (Scotch pine) ? $ G ! U A?G!U $ Tomicus piniperda ;' &''| T. Kirisits & ; < & Sordariomyceta ' G et al. - /012[4 5 !>B _;'< !AG &&_|&& 2015). I I\[>_|q'& > P[\ ! et al. 2008) 4 > $ q =; ^ v # K et al. ;<<_ ?4?GE?G $= ; ; NUCLEOTIDE SEQUENCE ACCESSION Neurospora crassav G et NUMBER al. ;<<* E } Grosmannia galeiformis !>B _;'< !AG &&_|&& 33AJ[>A# RESULTS AND DISCUSSION 4 A \B[<<<<<<<< E $ $ \B[<&<<<<<<. U$ ;_ &<< ^ E +*' $ _<< K E METHODS had a N50 of *| |' P K ;* qq > A?G!U 4 3F " K ^ '| 3x_ + %x& + >x< * n &=q+ !x v G. galeiformis !>B 3x v %x v >x _;'< 3F " $ 3 et al ;<&= 4 } Leptographium s. lat. $ @ D G } ;<<< B et al. 2015a, 2016). Genome annotation using ? $ ! 3 $ ! E =_< > P[\ 4 > ?4?GE?G __< K } +_;| ^ &<< ^ U ^ The genome of G. galeiformis E $ < =* A et al. 2014), and de novo GI $ = ' < A $ et al. B et al. ;<&_ ;<&* ;<&; G GI $ $ GGI ![^G $ = < A K studies of these fungi (Fig. 7). VOLUME 9 · NO. 2 413 et al. Authors: { ' Y@7 6 ^ _ / A [ !J D E! ;<<_ @ ; _ Y " 748 9;< 0< [=>80 *Contactx E 3 z K Mycologia 97x _|M*' A AP &'_& G Ceratocystis A Mycological ARTICLE Paper 35x &M&* ACKNOWLEDGEMENTS A $ F G $ 3 4 $ 3$ > et al. ;<&; GI x E Claviceps purpurea s.lat O ^ } Journal of Computational I $ \ > E # Biology 19x q__Mq|| >E# U \ ] 3 $ ! A I ;< 414 IMA FUNGUS IMA Genome – F10 Persoonia 22x =+Mq+ [ ! \ G D B > ` `, et al. (2012) Mind ARTICLE 3 \ &'_< U Coffea robusta F x I A \G ^ Caledonia. Revue Generale de Botanique 57x &M&& } PLoS ONE 7: e47768. 3 3 E 4# 3 \ I 3 ;<&; > E ;x % K # P A P G ^> > A > Nature S, et al. ;<&+ G K Methods 9: 772. ^ 3 A OB A 3 A \ I 4G ! IB et al. BMC Genomics ;<<* I Quambalariaceae $ 19x =+& Eucalyptus G . % J4 &'*+ The Day of St. Anthony’s Fire: the suspenseful, Studies in Mycology 55x ;+'M;'+ true account of a medieval plague in modern times, and of the 3 A OB 3 E A @ B A3 B >J ;<&q <9041 0[9 54 49 0 4 [ 8 \ 8 945 0 < 8]80<012 9 <4 F \ Ceratocystis Studies in Mycology x > 79x &+|M^;&' 4 R ^ % U $ P ! J ! K #> 4 K G 3 A OB > K G 3 E B >J ;<&| Bretziella, et al ;<&; x $ " ^ } Ceratocystis alignment data. Bioinformatics 28x;*|+M;*|' fagacearum (Microascales, Ascomycota). MycoKeys 27x &M&' 4 3> ;<&+ 4 } 3 A OB B >J ;<&= [ Fusarium verticillioides. Microbiology Resource Announcements Ophiostomatales. In: The Ophiostomatoid Fungi: expanding 7x <<'&+^&+ frontiers G P et al. x ;&Mq* !AG A $ G 4 3> E A !B A G[ A >P et al. &; ? x !AG^PF B % A $ @ ;<&= U x 3 Fusarium 3 4 FJ ` 3 IBJ ` EA D ` $ \ J &''+ [ x $ Phytopathology 103x q<<^q<+ 4 %G 3 I> P D O I A et al. " Drugs 56x _;=M_=_ ;<&| 3 $ K ^ 3 ` # > B >J 3 [, 4 E\ et GG\ Puccinia graminis al. ;<<| ! f. sp. avenae. Plant Pathology 67x q_|Mq** Fusarium circinatum and Fusarium subglutinans. 4 E\ G J ] U 3 &''* I Fungal Genetics and Biology 44: 701M714. Fusarium subglutinans f. sp. pini, in 3 ` # G [E > GD G ! % 4 et al. ! Mycological Research 100x +_ %1 G Southern Forests 78x &;=M&;' Fusarium circinatum 4 G 3 J^% # ` $ > D B et al. and Fusarium subglutinans. Fungal Biology 115: 902M'<+ ;<&< F " ^ 3 !D I P> J 3 A > !D G J et al. x I ># ;<&| ! $ = < Systematic Biology 59x =<|M=;& \ Venturia inaequalis and V. pirina 4 G 4 U ;<<= $ " $ " Systematic BMC Genomics 18x ==' Biology 52x *'*M| VOLUME 9 · NO. 2 415 et al. J D # G ! 4J # G G^D et al ;<&= 3 ` } Fusarium fujikuroi B14, the causal agent of the Genome Biology 5x \&; Genome Announcements 1x <<<=_^ # A G K G# ;<&; % ^ &= A ; Nature Methods 9x =_|M=_' P P G 3> ;<&= > %%E } # J% G A ;<<* The Fusarium Laboratory Manual. ARTICLE $ |x @ $ @ x B ^A Molecular Biology and Evolution 30: 772M|+< # D 3 \ ;<<' % P > > \ B G ^D $ G ! > et al. A ^B Bioinformatics 25x &|_qM&|*< ;<&; 4 A x " # D D A B % E \ J et al. (2009) The K } } G > Bioinformatics data. Bioinformatics 28x &*q|M&*q' 25x ;<|+M;<|' P U P > G > B G ;<&& $ # \ 3 A OB 3 E F I I } B >J ;<&; Grosmannia and Leptographium spp. alignments. Bioinformatics 27x |_|M|*= ^ % P E >! ;<&| E " $ \ L. taigense $ Antonie van Leeuwenhoek > x 102x =|_M='' $ Jaminaea Sympodiomycopsis species. # K A I3 A $ > ;<&q @ Mycological Progress 16: q'_M_<_ \F ^G } P E > GJ A P G # # # et al. (2018) Nucleic Acids Research 42: e119. A $ # K E ^D $ ` ! A $ > ;<<_ of the fungal clade Ustilaginomycotina. Molecular Biology and 4 $ ^ Evolution 35x &+q 416 IMA FUNGUS IMA Genome – F10 > > G [D ;<&| [$ I K ! 3 A OB F B >J A \ ;<<+ ARTICLE in fungal plant pathogens. Nature Reviews Microbiology 15: 3 $ Quambalaria eucalypti |_*M||& ^[ ? Australasian Plant F BJ 3 E B A3 B >J 3 A OB ;<&+ Pathology 37x *< VOLUME 9 · NO. 2 417 et al. G [E > U> ! E B A3 B troafricana, Diplodia scrobiculata, Fusarium nygamai, Leptographi- >J ;<&q [$ um lundbergii, Limonomyces culmigenus, Stagonosporopsis tanac- fungus Fusarium circinatum G Plant Pathology 63: eti, and Thielaviopsis punctulata. IMA Fungus 6x ;==M;q+ _= ARTICLE (*and other methods). ` q < &< eucalypticola, Chrysoporthe cubensis, C. deuterocubensis, G > x G Davidsoniella virescens, Fusarium temperatum, Graphilbum E > \ " J $ B > E A B >J ;<&& fragrans, Penicillium nordicum, and Thielaviopsis musarum. IMA ^ Acacia mangium associated Fungus 6x q'=M_<* Ceratocystis manginecans and C. acaciivora sp. nov. in B A3 A 3P G [E # DJ 3 E et al. Indonesia. South African Journal of Botany 77x ;';M= 418 IMA FUNGUS ! # P K @ Plant Pathology Journal 3x '|M&
D # I3\ @ x Proceedings of International 3 U> J } ! $ Conference on the Impacts of Climate Change to Forest Pests and 4 } ^ Diseases in the Tropics, 8–10 October (Mohammed C, Beadle C, % ! R 4 % ; \ " J et al x |'M+q @ x [ > " [ E " J^<<<
A 4 ;<&< A# GE^[{I#U\^ 4 $ G $ $ ` ` F E 4 ;<&= ? GEx [\ BMC Bioinformatics 29: Evolutionary Biology 10: 8. &<|;M&<|_ 3 4 ` A 4 G A G et al. (2008) D A 3 \ E 4 G ;<&q 4 4x I x ^ $ & < x x 4 4. Nucleic Acids Research 36x Bq*_MBq*' D E! G J P 4 &''+ 4 $ 3 4B G >[ D P# B A I et al. Ceratocystis " ;<<+ > $ European Journal of Forest Pathology fungal pathogen Claviceps purpurea. Molecular Ecology 17: 28x ;&|M;;* ;;|*M;;+* D J \ G > > % ! >, et al. 3 JJ 3 J# &'+| 3F ;<&q \ " ^ } Phytochemistry Bulletin 190x&&M&_ } Genome Research 24x *++M*'* 3 E A OB B A3 B >J ;<&= D 4! ! IB ! J A E@ B >J ;<&& ! K ^ Leptographium Mycosphaerella and Teratosphaeria diseases of Eucalyptusv procerum and Leptographium profanum. Fungal Biology 117: } Fungal Diversity q&&Mq;& 50x &q_M&** [ \! ;<
?G!#[x } J E G G # B D J P D! et al. ;<&= Polyporales Nucleic Acids Research 32: $ &|';M&|'| Mycologia 105x &=|qM&='<
E % ! !# # GP et al. (2015) Chemistry 44x &;+_M&;'< @ K Quambalaria > [> A 4 G D 4 DD &''; cyanescens, 3F PLoS ONE 10x &M&_ P K G I 3 # G > G > et al. (2004) Nucleic Acids Research 20x *&&_M*&&*
Eucalyptus grandis in South Gibberella fujikuroi " Plant Pathology 53x q&qMq;& Mycologia 90x q*_Mq'= G P D D E % E ;<&q 3 } UR3 P F D@ E ! [ ;<<< Pseudozyma antarctica J!>&<=&| Gibberella fujikuroi "x Mycoscience 41: lipids. Genome Announcements 2x qM_ *&M|+ G J 4 > ] > % ;<&& Fusarium temperatum U P> I P E I B >J O ;<<= $ K Phaeophleospora destructans Fusarium subglutinans. Mycologia 103x _+*M_'| G [ Feb. ` ; M Offered papers ICPP: 165, G !# ! D ? G4 $ P et al. ! F O ;<&= I ^ x ^ I E A E@ > ! J G A# D 4[ ;<<+ $ $ Quambalaria species, including Q. coyrecup $ PLoS Genetics 9x &<<==;= Corymbia G I ;<<' [ x " " B Mycological Re- World Mycotoxin Journal 2x&q&M&q' search 112x _|M*' G % B \> @ I P $ $ [ ` O $ I \% P IJ B >J ! IB ;<<< % [> ;<&_ A?G!Ux @ x Diseases and Pathogens of ^ Bioinformatics 31: Eucalypts P IJ P 4 I %3 et al x &_=M;=' =;&
4 ^% *x Cercospora zeina, E $ G &'+* G Fusarium pininemorale, Hawksworthiomyces lignivorus, Huntiella 3F } @ x Lectures on Mathematics in the decipiens and Ophiostoma ips. IMA Fungus 8x =+_M='* Life Sciences 17x _|M+* > G \ B A3 A 4% 3 D B F BJ et al. (2018) Island. @> 4 ^% ' 3 } Annulohypoxylon E ^D $ ` # K ! U A $ > stygium, Aspergillus mulundensis, Berkeleyomyces basicola ;<<+ 4 $ ab . Thielaviopsis basicola), Ceratocystis smalleyi, initio $ Genome Research Cercospora beticola , Coleophoma cylindrospora, 18x &'|'M&''< Fusarium fracticaudum, Phialophora cf. hyalina, and Morchella E > P D A # K E et al ;<&q 3 septimelata. IMA Fungus 9x &''M;;= } $ Tilletiaria anomala B A3 3 E D ` F > B UBC 951. Genome Announcements 2x ;M= et al. ;<&* @> 4 ^% | 3 } ` # [J I $ P I $ G G \ $ [ % > Ceratocystis fagacearum, C. harringtonii, Grosmannia et al ;<&* [ Claviceps purpurea penicillata, and Huntiella bhutanensis. IMA Fungus 7x =&|M=;= G Fungal Biology 120x '&|M'=< B A3 G [E G ! ! K >I A ` F > A # ! J 3 J[ 3 A OB S, et al ;<&; % } x et al ;<&q 3 Amanita jacksonii, Ceratocystis South African Journal of Science 108x &M; albifundus, Fusarium circinatum, Huntiella omanensis, Leptogra- B A3 ` B > \ D B >J ;<&= Ceratocystis: phium procerum, Rutstroemia sydowiana, and Sclerotinia echi- $ @ x The nophila. IMA Fungus 5x q|=^q+* Ophiostomatoid Fungi: expanding frontiers G P 3 A ` B > B A3 B >J ;<&= Ceratocystis species OB B >J x _|M*q !AG A $ G &; in the 748 9;< 0< [=>80 " @ x The Ophiostomatoid ? x !AG^PF B % A $ @ Fungi: expanding frontiers G P 3 A OB B B >J ! IB A 3 &''* Kiiramyces destructans >J x *_M|= !AG A $ G &; ? x !AG^ $ Eucalyptus in Indonesia. PF B % A $ @ South African Journal of Botany 62x =;_M=;| ` B G B A3 3 ` # G ! ` > B >J ! IB G BJ &''= Sporothrix eucalypti (sp. F et al. ;<&+ > $ [ G Fusarium circinatum. IMA Mycopathologia 123x&_'M&*q Fungus 9x ;|M=* { J G !B D I 4 \ F E et al. (2007) B AJ E G E I > , et al. (2014) Pilon: 3 ^ Malassezia $ $ $ $ $ $ $ PLoS ONE 9x &&;'*= pathogens. Proceedings of the National Academy of Sciences, B F I E O J 3 { et al ;<&_ 3 USA 104x &+|=
P ` A P G # F [> et al. 96x &=<*M&=&_ ;<&= ? x ^ O O3 { J ! G% B >J ;<<+ 3 ^ Fusarium fujikuroi $ " ! x Fungal $ PLos Pathogens Diversity 32x &M+ 9x &<<=q|_ O \3 3 A OB J P B A3 B >J ;<<* B A3 IP ^F % A # A B et al. (2015a) > Ceratocystiopsis and Grosmannia @> 4 ^% q 3 } Chrysoporthe aus- Ophiostoma. Studies in Mycology 55x |_M'|