Cite This: Environ. Sci. Technol. 2018, 52, 1234−1243 pubs.acs.org/est
Kinetics of Decomposition of Thiocyanate in Natural Aquatic Systems Irina Kurashova,† Itay Halevy,‡ and Alexey Kamyshny, Jr.*,†
† Department of Geological and Environmental Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel 84105 ‡ Department of Earth and Planetary Sciences, Weizmann Institute of Science, Rehovot, Israel 76100
ABSTRACT: Rates of thiocyanate degradation were measured in waters and sediments of marine and limnic systems under various redox conditions, oxic, anoxic (nonsulﬁdic, nonferruginous, nonmanganous), ferruginous, sulﬁdic, and manganous, for up to 200-day period at micromolar concentrations of thiocyanate. The decomposition rates in natural aquatic systems were found to be controlled by microbial processes under both oxic and anoxic conditions. The Michaelis−Menten model was applied for description of the decomposition kinetics. The decomposition rate in the sediments was found to be higher than in the water samples. Under oxic conditions, thiocyanate degradation was faster than under anaerobic conditions. In the presence of hydrogen sulﬁde, the decomposition rate increased compared to anoxic nonsulﬁdic conditions, whereas in the presence of iron(II) or manganese(II), the rate decreased. Depending on environmental conditions, half-lives of thiocyanate in sediments and water columns were in the ranges of hours to few dozens of days, and from days to years, respectively. Application of kinetic parameters presented in this research allows estimation of rates of thiocyanate cycling and its concentrations in the Archean ocean.
■ INTRODUCTION aquatic systems, as well as to chemical transformations, 24−26 Thiocyanate (NCS−) is formed in various natural and industrial volatilization and adsorption. processes. It was found in waste waters from coal and oil The presence of thiocyanate was recorded in various natural nonpolluted aquatic systems, for example in stratiﬁed lakes processing, steel manufacturing, and the petrochemical −1 1−5 · Rogoznica in Croatia (up to 288 nmol·L ) and Green Lake industry. Thiocyanate concentrations of up to 17 mmol −1 19 −1 4,6 (NY, USA) (up to 274 nmol·L ). In these lakes thiocyanate L were detected in coal plant wastewaters and in gold ﬁ ﬀ extraction wastewaters.7,8 In electroplating, dyeing, photo- is produced in the anoxic, sul de-rich sediments and di uses to ﬁnishing, thiourea, and pesticide production the concentration the chemocline, where it is consumed by oxidative processes, of NCS− in wastewater eﬄuents is in the range of 0.09−2 which are likely biologically enhanced. In the oxic North Sea · −1 9 water thiocyanate was detected at concentrations up to 13 mmol L . Thiocyanate is toxic to aquatic species with LC50 −1 16 −1 10 nmol·L . In saltmarsh sediment of the Delaware Great Downloaded via WEIZMANN INST OF SCIENCE on September 20, 2018 at 06:31:37 (UTC). values of 0.01 to 0.5 mmol·L reported for Daphnia magna. μ · −1 See https://pubs.acs.org/sharingguidelines for options on how to legitimately share published articles. Natural sources of thiocyanate include plants, biological and Marsh, thiocyanate concentrations are up to 2.28 mol L in μ · −1 26 abiotic decomposition of organic matter, and in vivo pore-water and up to 15.6 mol kg in wet sediment. In − detoxiﬁcation of cyanide.11 14 Several species of bacteria, these sediments, concentrations of thiocyanate precursors, algae, fungi, plants, and animals are physiologically capable of cyanide-reactive zerovalent sulfur and free hydrogen cyanide are μ · −1 μ · −1 detoxifying cyanide, and in most cases one of the end products as high as 78 mol L and 1.9 mol L , respectively. ﬁ of detoxiﬁcation is thiocyanate.10,15 Another important Coexistence of hydrogen cyanide, polysul des and thiocyanate mechanism of thiocyanate formation in natural aquatic systems allows attribution of thiocyanate formation to the abiotic is the reaction between hydrogen cyanide and sulﬁde oxidation reactions between hydrogen cyanide and reduced sulfur species. − μ · −1 intermediates, which contain sulfur−sulfur bonds, such as Thiocyanate was also found in concentrations 23 40 mol L 27 colloidal sulfur,16 polysulﬁdes,17 thiosulfate, and tetrathio- in the Red Sea Atlantis II Deep brine. Reactions between nate.18,19 Reaction with thiosulfate is ∼1000 times slower abiotically formed hydrogen cyanide and reduced sulfur species than reaction with polysulﬁdes,17,20,21 and is catalyzed by were proposed as the source of thiocyanate in the brine. The Cu(II) and sulfur transferases from the rhodanese family.15,22 Concentrations of hydrogen cyanide in polluted aquatic Received: September 17, 2017 systems may reach levels toxic to aquatic life.23 In nonpolluted Revised: December 25, 2017 aqueous systems, hydrogen cyanide concentrations are usually Accepted: December 28, 2017 very low due to fast degradation by plants, fungi and bacteria in Published: December 28, 2017
© 2017 American Chemical Society 1234 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
Table 1. Rates of Thiocyanate Degradation in Natural and Controlled Systems
− · −1 μ · −1· −1 system conditions [NCS ]o (mmol L ) rate ( mol L d ) ref Lake Kinneret and Mediterranean Sea water oxic 0.003 0.026−0.23 this work anoxic 0.003 0.0048−0.021 this work Lake Kinneret and Red Sea sediment oxic 0.003 2.1−2.4 this work anoxic 0.003 0.0074−0.023 this work gold mine tailing storage facility microbial consortium in synthetic medium oxic 0.003 ∼5.4 43 activated sludge mixed liquor oxic 0.003 110 72 wastewater with cultivated microorganisms oxic 0.003 2800 36 Lake Kinneret and Mediterranean Sea water oxic 1 0.43−0.90 this work anoxic 1 0.034−0.34 this work Lake Kinneret and Red Sea sediment oxic 1 45−49 this work anoxic 1 0.078−0.61 this work gold mine tailing storage facility microbial consortium in synthetic medium oxic 1 ∼1700 43 activated sludge mixed liquor oxic 1 2100 72 wastewater with cultivated microorganisms oxic 1 82500 36 water enrichment with Klebsiella species oxic 0.4−3.9 ∼860 79 enrichment cultures from steel plant wastewater oxic 5.7 ∼1.9 53 enrichment cultures from steel plant wastewater anoxic 4.7−5.6 ∼0.79 53 activated sludge anoxic 1.7 0 3 brine liquor with mixed methanogenic cultures anoxic 2.5 0 80 a simulated wastewater containing phenol, thiocyanate, and ammonia-nitrogen anoxic 1.9−10.3 0 81 same processes were proposed to result in thiocyanate the rates may be as high as 83 mmol·L−1·d−1 at 1 mmol·L−1 formation in the hydrothermal springs and pools in the concentration of thiocyanate (Table 1). Thiobacillus species Yellowstone National Park, where it was detected at isolated from steel plant wastewater, are able to degrade 5.2 − − concentrations 133−791 μmol·L−1.28 mmol·L 1 of NCS over 8 days under anaerobic denitrifying Although concentrations of thiocyanate in modern aquatic conditions.53 Rates of thiocyanate degradation depend not only systems are low,16,19,26,28 this may be not the case for the iron- on oxygen concentrations,3,53,80,81 but also on concentrations of rich water column of the Archean ocean. Existence of large nutrients. It was shown that concentrations of NH >70μmol· − 3 atmospheric ﬂuxes of both hydrogen cyanide and zerovalent L 1 may inhibit76 and phosphate concentrations >500 μmol· − − 8 sulfur to Archean ocean has been suggested.29 34 These works L 1 may stimulate thiocyanate biodegradation. provide constraints on cyanide and zerovalent sulfur ﬂuxes but Currently, metabolic pathways for thiocyanate degradation lack of data regarding rates of thiocyanate degradation under are known and the rates of its degradation in bioreactors are environmental conditions in the presence of dissolved iron(II) published, but no data on kinetics of decomposition of prevents estimation of thiocyanate concentrations and its fate in thiocyanate in natural aquatic systems is available. The main ﬁ the Archean ocean. aim of the present research is to ll this gap and to provide Multiple investigations focused on the isolation and constraints on rates of decomposition of thiocyanate in limnic identiﬁcation of microorganisms that are responsible for and marine water columns and sediments at various redox − thiocyanate biodegradation under controlled conditions.35 52 conditions, which are relevant for modern and ancient natural Thiocyanate-degrading bacteria have been isolated from aquatic systems. To achieve this goal, we have determined activated sludge,41 steel plant wastewater,53 gold mine tailing kinetic parameters of thiocyanate degradation in natural aquatic sites,43,50,51 and extreme alkaline54 and hypersaline55,56 environ- and sedimentary systems at various salinities and concen- − ﬁ ments. The end products of NCS degradation are ammonium, trations of thiocyanate, oxygen, hydrogen sul de, and iron(II). carbon dioxide, and sulfate, subsequent to the formation of − − cyanate57 62 or carbonyl sulﬁde63 67 as intermediates. A variety ■ MATERIALS AND METHODS − of autotrophic bacteria are capable of using NCS as their sole Materials. Solutions for measurements of the kinetics energy source via sulfur oxidation. Chemolithotrophic bacteria parameters of thiocyanate degradation were prepared in Milli-Q ﬁ − utilize hydrogen sul de formed on the initial step of NCS water, Mediterranean Sea water, Red Sea water, Lake Kinneret degradation and products of its incomplete oxidation (Lake Tiberias) water. Slurries were prepared from Red Sea and (polysulﬁde, elemental sulfur, or thiosulfate) as an energy Lake Kinneret sediments. All reagents were purchased from 41,63,68,69 source for growth, oxidizing sulﬁde to sulfate. Sigma-Aldrich and were at least 98% pure. Potassium Utilization of nitrogen released from thiocyanate as ammonia thiocyanate (KSCN) (99%), sodium sulﬁde nonahydrate 58 · has also been reported. A number of heterotrophic bacteria (Na2S 9H2O) (98%), and iron(II) sulfate heptahydrate − 46,50,52,70,71 · ﬁ utilize NCS as a source of nitrogen and sulfur as (FeSO4 7H2O) (99%) were used without further puri cation. well as energy source.38,44 Heterotrophic bacteria were shown Nitrogen gas (99.999%) was purchased from Maxima ltd, Israel. to utilize thiocyanate at concentrations as high as 100 mmol· Test tubes, screw caps with hole and septum, and aluminum − L 1.70 caps were purchased from Romical, Ltd., Israel, and Labco, Ltd., In biotechnological systems for thiocyanate removal, which UK; butyl rubber stoppers were purchased from Geo-Microbial are operating under high concentrations of thiocyanate (up to Technologies, Inc., USA. − − 120 mmol·L 1 and oxic conditions10,36,43,72 79), rates of Samples Preparation and Treatment. Water and thiocyanate decomposition are high. Under oxic conditions sediment samples were collected from various locations in
1235 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
Table 2. Sampling and Processing Protocols for Preparation of Water Samples
sample depth initial no. system (m) sampling date conditions treatment ﬁnal conditions WC-1 Lake Kinneret 1 17 Jun, 2015 oxic no oxic, pH 8.1 ﬁ μ · −1 μ · −1 WC-2 Lake Kinneret 37 17 Jun, 2015 sul dic N2 purge [O2]<1 mol L ,[H2S] < 1 mol L , pH 7.8 ﬁ ﬁ μ · −1 WC-3 Lake Kinneret 37 17 Jun, 2015 sul dic no sul dic ([H2S] = 35 mol L ), pH = 7.7 ﬁ μ · −1 WC-4 Lake Kinneret 37 17 Jun, 2015 sul dic N2 purge, FeSO4 spiking ferruginous ([Fe(II)] = 81 mol L ), pH 7.9 WC-5 Mediterranean Sea 1 17 May, 2015 oxic no oxic, pH 8.4 μ · −1 WC-6 Mediterranean Sea 1 17 May, 2015 oxic N2 purge [O2]<1 mol L , pH 8.4 ﬁ μ · −1 WC-7 Mediterranean Sea 1 17 May, 2015 oxic N2 purge, Na2S spiking sul dic ([H2S] = 118 mol L ), pH 8.3 μ · −1 WC-8 Mediterranean Sea 1 17 May, 2015 oxic N2 purge, FeSO4 spiking ferruginous ([Fe(II)] = 95 mol L ), pH 8.7
Israel (Tables 2 and 3). Sediment cores and water samples were were purged with nitrogen under a nitrogen atmosphere in a collectedfromtheRedSea(29°30′N, 34°55′E), the glovebag. Sulﬁdic conditions were simulated by addition of the Mediterranean Sea (32°49′N, 34°59′E), and Lake Kinneret sodium sulﬁde as described above. After distribution to the test (32°50′N, 35°35′E) in May 2015−December 2016. Sediment tubes, slurry samples were spiked by KCSN solution to the samplings were performed with a multicorer, Lake Kinneret, target concentrations of NCS− in slurry pore-waters: 3, 10, 30, and Red Sea waters were sampled with Niskin bottles, 100, 300, and 1000 μmol·L−1.TheratesofNCS− Mediterranean Sea water was sampled manually near the decomposition at concentrations >1 mmol·L−1 were not shore. The water and cores were stored at 25 ± 1 °C in the measured as (1) these concentrations are not relevant for dark and processed in <1 day after sampling. natural aquatic systems and (2) some works36,73 report that at The rates of decomposition of thiocyanate in water were these concentrations the microbial decomposition of thiocya- measured in the following sets of samples: (1) oxic water nate is substrate-inhibited; although there is also evidence for sample without pretreatment; (2) anoxic, nonsulﬁdic water high thiocyanate decomposition rates at higher concentrations. samples, which were stripped of oxygen or hydrogen sulﬁde by For each water and sediment sample, an identical sample purging for 90 min with nitrogen in a glovebag; (3) ferruginous, autoclaved at 120 ± 3 °C for 20 min was prepared in order to prepared by adding of iron sulfate to the target concentration provide a sterilized control. All types of autoclaved samples 100 μmol·L−1 of Fe(II); (4) sulﬁdic, prepared by adding were prepared in 35 mL (water samples) and in 50 mL sodium sulﬁde to the target concentration 100 μmol·L−1 of (sediment samples) tubes and were hermetically closed with a μ · −1 S(II) and 50 mol L H2SO4 for pH adjustment (Table 2). rubber stopper and aluminum cap. Nonautoclaved water samples were distributed to 12 mL test All sterilized and nonsterilized samples were prepared and tubes. The tubes were ﬁlled without a headspace and analyzed in triplicates. Hermetically closed samples were hermetically closed using a screw cap with hole and septum. incubated at 25 ± 1 °C. Subsamples were taken from the KSCN was injected into the test tubes with samples to the samples by syringes immediately prior to analysis. Subsampling following target concentrations: 3, 10, 30, 100, and 300 μmol· of anoxic samples was carried out in the glovebag (typically −1 L . <0.2% O2 v/v) to maintain anoxic conditions in the sample. The rates of decomposition of thiocyanate in the sediments The rate of thiocyanate decomposition was studied by were measured in the samples from the locations presented in measurement of thiocyanate concentrations in multiple Table 3. Both Red Sea (Gulf of Aqaba) water and sediment subsamples during 35−200 day period. cores were sampled at 561 m water depth. The cores were Analytical Methods. Thiocyanate was quantiﬁed by an sectioned into slices: 1−2 cm (oxic), 8−12 cm (manganous), Agilent Technologies 1200 HPLC system with multiple 20−24 cm (ferruginous), and 30−34 cm (anaerobic zone with wavelength UV−visible detector operated at 220 nm. A 2+ 2+ μ · −1 ﬁ [H2S], [Fe ], [Mn ] < 1.5 mol L ). Sul dic Lake Kinneret Nomura Chemical, Japan, Develosil RPAQUEOUS C30 water and sediment cores were sampled below the chemocline reverse phase column (150 mm × 4.6 mm × 5 mm) was at 37 m depth. The cores were sectioned into slices: 5−10 cm used for HPLC separation of thiocyanate. The column was ﬁ − 2+ 2+ ﬁ 82 (sul dic), 12 17 cm (anaerobic zone, [H2S], [Fe ], [Mn ]< modi ed according to Rong et al. by pumping 5% aqueous 2.5 μmol·L−1), and 20−25 cm (ferruginous zone). Additional solution of PEG-20 000 at 0.3 mL·min−1 rate for at least 3 h. core was sampled above the chemocline at 32 m water depth Hydrogen sulﬁde was measured spectrophotometrically by the for oxic (upper 0−5 mm) sediment layer. methylene blue method according to Cline83 by LaMotte For experiments under anoxic conditions, the cores were cut SMART Spectro spectrophotometer. Fe2+ was quantiﬁed under a constant ﬂow of nitrogen and immediately transferred spectrophotometrically by ferrozine method according to 84 to a glovebag with N2 atmosphere. For killed control Stookey. Manganese was analyzed spectrophotometrically experiments, marine and limnic waters were sterilized by using the 1-(2-Pyridylazo)-2-naphthol (PAN) method.85 Oxy- autoclaving at 120 ± 3 °C for 20 min to prevent the eﬀect of gen level was monitored using an optical oxygen meter with a microbial community from the water column on the Retractable Needle-Type Oxygen microsensor (tip diameter degradation rate in sediment samples. Sediments were diluted ∼50 μm, MDL of 0.625 μmol·L−1, Firesting-Pyroscience) (1:1) with autoclaved bottom water (except for oxic Kinneret placed on a micromanipulator.86 Data was collected via sediment, which was diluted with autoclaved oxic water from 1 microproﬁling software (Proﬁx) with 2-point calibration. The m depth) and transferred to the 50 mL test tubes, which were optode needle was inserted into the sample through the septum hermetically closed using a hand crimper with a rubber stopper of the HPLC-vial which was ﬁlled and closed in the glovebag. and aluminum cap. For experiments under simulated anoxic Approach for Estimation of NCS− Degradation Rate. conditions, autoclaved oxic seawater and sulﬁdic limnic water Quantitative interpretation of biodegradation kinetics is based
1236 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
on substrates degradation via catalyzed reactions, which are carried out by the organisms bearing the speciﬁc enzymes. , pH 7.6 , pH 8.4 1 1 Therefore, rates of substrate degradation are generally depend- − − L L · · ent on the enzyme and substrate concentrations (e.g.,
mol mol Michaelis−Menten kinetics model). μ μ ), pH 8.6 1
− In natural aquatic systems degradation of thiocyanate may be L · performed by a single organism, as well as by microbial
μ consortium. It has been shown that the Michaelis Menten model87 (eq 1) can be applied for description of microbial , [Mn(II)] = 0.5 , [Mn(II)] = 0.5
1 1 utilization of cyanide and thiocyanate in activated sludge − − ), pH 8.7
1 3 L L ), pH 8.3 ), pH 7.8 · ·
− ﬀ 1 1 reactors, as well as for determination of the e ect of L − − · L L mol mol · · concentration of organic pollutants on their biodegradation μ μ , [Mn(II)] = 0.5), pH 7.5 , [Mn(II)] = 1 mol
1 1 88 μ − − mol mol rate by natural microbial communities. Thus, in this study we L L μ μ · · will deﬁne “apparent” parameters of Michaelis−Menten model,
mol mol ﬁ μ μ which correspond to degradation of thiocyanate by nonde ned 63
nal conditions microbial community. Katayama et al. showed that ﬁ , [Fe(II)] = 0.5 , [Fe(II)] = 0.5 1 1 thiocyanate-degradation catalyzed by enzyme isolated from − − L L · · , [Mn(II)] = 2 , [Mn(II)] = 2.5 − 1 1
, [Mn(II)] = 35 Thiobacillus thioparus cells, followed Michaelis Menten-type − − 1 − L L mol mol
· · − L kinetics. In this work, the Michaelis Menten model was μ μ · , [Fe(II)] = 0.5 , [Fe(II)] = 1.5 1 1 mol mol
− − applied to nonautoclaved samples. mol μ μ L L μ · · S] = 2.5 S] = 1.5 mol mol 2 2 [S]V μ μ V = max ,[H ,[H 1 1 [S] + K (1) − − L L · · S] = 39 S] = 75 2 2
mol mol where V is the rate of thiocyanate decomposition at the given μ μ μ · −1· −1 concentration ( mol L day ), Vmax is the apparent maximum −1 −1 dic ([H dic ([H ]<1 ]<1 μ · · 2 2 ﬁ ﬁ rate of thiocyanate decomposition ( mol L day ), K is the
sul apparent half-saturation constant (corresponding to the S
2 substrate concentration at which the reaction rate is 50% of μ · −1 the Vmax ( mol L ), and [S] is the concentration of substrate (μmol·L−1). Speciﬁc degradation rate at each initial substrate concen- tration was analytically estimated by ﬁtting the data for decrease dic water sul ﬁ -purged water-purged water [O ferruginous-purged ([Fe(II)] water = 40 [O -purged water-purged water ferruginous ([Fe(II)] = 37 manganous ([Fe(II)] = 2 of concentration versus time. An overall dependence was -purged water, Na 2 2 2 2 2 2 established by plotting the calculated thiocyanate degradation rates versus initial thiocyanate concentrations. For estimation of the speciﬁc degradation rate, reaction rate was measured only
spiking on the initial stage of thiocyanate degradation, where thiocyanate degradation rate had linear dependence on time. ﬁ Apparent Vmax and K values were calculated by tting curves to data using nonlinear regression and performing a nonlinear ﬁt dic dilution with sul initial ﬁ analysis in MATLAB. conditions treatment ferruginous dilution with N manganous dilution with N anoxic dilution with N oxicanoxic dilution with water dilution with N oxic, pH 8.8 ■ RESULTS AND DISCUSSION In all sterilized samples (water and sediments), the variation of date 2016 2016 2016 2016 2016 sampling thiocyanate concentration was <3% of initial concentration during the whole incubation period (data not shown). This indicates chemical stability of thiocyanate in aquatic systems in 24 20 Sep, 34 20 Sep, 25 1 Dec, 2016 ferruginous dilution with N 34 20 Sep, 17 1 Dec, 2016 anoxic dilution with N 12 20 Sep, 10 1 Dec, 2016 sul 2 20 Sep, 0.5 1 Dec, 2016 oxic dilution with water oxic, pH 8.0 − − − − − a wide range of environmentally relevant conditions, for − − − − (cm)
depth bsf example, in the presence as well as in the absence of sediments, oxygen, hydrogen sulﬁde, and dissolved iron in marine, as well as in limnic waters. (m)
depth In all nonautoclaved samples with initial thiocyanate concentrations of 3 μmol·L−1, decomposition was not observed during the ﬁrst 26−100 days of incubation, except for oxic sediments and some oxic Mediterranean seawater samples in which the lag periods were <12 h and <12 days, respectively (Table 4). With an increase of NCS− concentrations, the lag period increased by 25−63%. After the lag period, the thiocyanate concentrations decreased in all nonsterilized ﬂ no. system S-8 Red Sea 561 20 S-9 Red Sea 561 8 S-3 Lake Kinneret 37S-7 5 Red Sea 561 30 S-4 Lake Kinneret 37 20 S-5S-6 Red Sea Red Sea 561 561 0 30 S-2 Lake Kinneret 37 12 S-1 Lake Kinneret 32 0 samples. The lag period likely re ects the time which is sample
Table 3. Sampling and Processing Protocols for Preparation of Sediment Samples required for adaptation to a new substrate and an increase in
1237 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
Table 4. Lag Times for Thiocyanate Decomposition at 3 μmol·L−1 Concentration
ﬁnal conditions system time oxic anoxic sulﬁdic ferruginous manganous Lake Kinneret water incubation period (d) 156 164 112 142 lag time (d) 37 74 58 72 Mediterranean Sea water incubation period (d) 164 164 130 141 lag time (d) <12 79 71 75 Lake Kinneret sediment incubation period (d) 35 150 150 150 lag time (d) <1 46 35 45 Red Sea sediment incubation period (d) 35 157 157 157 157 lag time (d) <1 35 26 45 45
a Table 5. Coeﬃcients of the Michaelis−Menten Equation
ﬁ μ · −1 μ · −1· −1 sample no. system initial conditions nal conditions K ( mol L ) Vmax ( mol L day ) WC-1 LK oxic oxic 48 ± 11 0.450 ± 0.032 WC-2 LK sulﬁdic anoxic 59 ± 17 0.145 ± 0.014 WC-3 LK sulﬁdic sulﬁdic 48 ± 13 0.355 ± 0.030 WC-4 LK sulﬁdic ferruginous 29 ± 10 0.052 ± 0.006 WC-5 MS oxic oxic 9 ± 3 0.905 ± 0.07 WC-6 MS oxic anoxic 31 ± 8 0.167 ± 0.010 WC-7 MS oxic sulﬁdic 75 ± 20 0.251 ± 0.023 WC-8 MS oxic ferruginous 18 ± 3 0.035 ± 0.006 S-1 LK oxic oxic 61 ± 10 52 ± 3 S-2 LK anoxic anoxic 57 ± 20 0.351 ± 0.029 S-3 LK sulﬁdic sulﬁdic 89 ± 29 0.663 ± 0.039 S-4 LK ferruginous ferruginous 28 ± 8 0.081 ± 0.006 S-5 RS oxic oxic 65 ± 24 48 ± 2 S-6 RS anoxic anoxic 58 ± 12 0.356 ± 0.019 S-7 RS anoxic sulﬁdic 69 ± 21 0.575 ± 0.044 S-8 RS ferruginous ferruginous 55 ± 18 0.155 ± 0.014 S-9 RS manganous manganous 51 ± 14 0.166 ± 0.021 aWC, water column; S, sediment; LK, Lake Kinneret; MS, Mediterranean Sea; RS, Red Sea. cell concentrations as a response to thiocyanate amend- diﬀerence in thiocyanate decomposition rates in the absence ment.43,51 and in the presence of dissolved iron and hydrogen sulﬁde may On the basis of the speciﬁc degradation rate at each initial be attributed to diﬀerences in microbial community structure in substrate concentration, the apparent kinetic parameters of the thepresenceofdiﬀerent electron donors. Calculated Michaelis−Menten model for each analyzed system were concentrations of thiocyanate-iron complex, Fe(SCN)+, are determined (Table 5). Experimental rates and the model data μ · −1 − <0.08 mol L . This concentration corresponds to the sample versus initial NCS concentration were plotted; the thiocyanate with the highest concentrations of Fe2+ (95 μmol·L−1) and − ﬁ − decomposition rates and their Michaelis Menten model ts are thiocyanate (1 mmol·L 1) and the stability constant of 0.84 − − presented in Figure 1. Apparent parameters of the Michaelis mol 1·L for Fe(SCN)+ complex.91 Therefore, iron complex- Menten model were used to calculate thiocyanate half-lives at ation should not signiﬁcantly aﬀect the observed decrease of various concentrations (Figure 2). The lag time was not iron or thiocyanate concentrations. included in determination of the half-life time. − Comparison between Marine and Limnic Systems. In oxic Degradation of NCS in Water Columns. Inﬂuence of Mediterranean Sea water, the thiocyanate half-life was shorter redox conditions. The thiocyanate degradation rates in Lake than in oxic Lake Kinneret water. We attribute this diﬀerence to Kinneret waters decreased in the following order: oxic > sulﬁdic faster adaptation of local microbial communities in seawater to > anoxic > ferruginous (Figures 1a and 2a). In seawater − samples, the trend was similar: oxic > sulﬁdic ≈ anoxic > an increase in NCS concentrations. This explanation is ferruginous (Figures 1band2a). Relatively low rate of supported by longer lag periods observed in the case of oxic thiocyanate degradation under anaerobic conditions is charac- limnic water. Another support for this explanation comes from teristic for thiocyanate-decomposing bacteria and may be comparison of thiocyanate degradation rates in the presence of ﬁ ﬁ explained by lower yield of metabolic energy associated with hydrogen sul de. In Lake Kinneret water, which was sul dic utilization of electron acceptors, which are weaker than during the sampling, degradation rates were higher than in the oxygen.53,59,89 Since the only electron acceptor proven to Mediterranean Sea water, which was oxic at the time of support thiocyanate oxidation by bacteria in the absence of sampling but was amended with hydrogen sulﬁde. In the latter oxygen is nitrate, degradation of thiocyanate in these systems is case, the microbial community has to adapt to drastically likely to be nitrate-limited. In the anoxic waters of Lake changed conditions. Our observations show that the inﬂuence Kinneret, nitrate concentrations are <37 μmol·L−1, but can be of salinity on thiocyanate degradation rates is less pronounced as well <4 μmol·L−1 in the benthic boundary layer.90 The for all systems than inﬂuence of oxygen.
1238 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
Figure 1. Thiocyanate degradation rates as a function of the initial thiocyanate concentration in Lake Kinneret water (a) and Mediterranean Sea water (b), in oxic Lake Kinneret and Red Sea sediments (c), anoxic Lake Kinneret sediment (d), and anoxic Red Sea sediment (e). Open symbols represent experiment data; solid symbols represent apparent K of the Michaelis−Menten equation; broken lines represent the model based on the apparent Michaelis−Menten kinetic parameters. Black color represents samples under oxic conditions, red color represents samples under anoxic conditions, green color represents samples under sulﬁdic condition, blue color represents samples under ferruginous conditions, and purple color represents samples under manganous conditions.
1239 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
thiocyanate degradation. This observation agrees well with the results reported by Hung and Pavlostathis80 and Sahariah and Chakraborty,81 who found that thiocyanate is stable under the methanogenic conditions. Comparison of Thiocyanate Degradation Rates in Natural Systems and under Controlled Conditions. Compar- ison of thiocyanate decomposition rates reported in present work and those calculated for the same range of thiocyanate concentrations from kinetic parameters measured under controlled conditions are presented in Table 1. The rates of decomposition in nonpolluted natural aquatic systems are expected to be much lower than in the industrial systems due to (1) much lower microbial density and (2) lower concentrations of nutrients. Indeed, rates of thiocyanate decomposition in oxic sediments were found to be lower by 2−4.5 orders of magnitude as compared to activated sludge and wastewater microbial cultures.36,43,72 Under anoxic conditions, NCS− decomposition generally was not detected in industrial systems,3,80,81 except for steel plant wastewater with high concentrations of NCS− (4.7−5.6 mmol·L−1).53 Rates of thiocyanate decomposition calculated from the data presented by Grigor’eva et al.53 are only slightly higher than those detected in the anoxic sediments in this work at 1 mmol·L−1 concentration (Table 1). Although mechanisms and pathways of thiocyanate degra- dation under controlled conditions are well studied, detailed understanding of processes controlling stability of thiocyanate in natural aquatic systems is lacking. Future work should include detection of microbial species responsible for thiocyanate degradation in natural aquatic systems, evaluation of microbial community shifts as a response to thiocyanate Figure 2. Estimated half-lives of thiocyanate degradation in aquatic (a) pollution and identiﬁcation of speciﬁc metabolic pathways of and sedimentary systems (b). Solid lines represent seawater samples; thiocyanate degradation under various environmental con- dashed lines represent limnic samples. Black color represents samples ditions. under oxic conditions, red color represents samples under anoxic − conditions, green color represents samples under sulﬁdic condition, Implications for the Archean Oceans. The Michaelis blue color represents samples under ferruginous conditions, and Menten model for the ferruginous Mediterranean seawater was purple color represents samples under manganous conditions. appliedtoestimatethesteady-state concentration of thiocyanate in the Archean Ocean. Ferruginous conditions Degradation of NCS− in Sediments. Inﬂuence of Redox may have been a dominant characteristic of the anoxic oceanic 98 Conditions. In both marine (Red Sea) and limnic (Lake water column throughout much of Earth’s history. Based on Kinneret) sediments, NCS− degradation was found to be faster estimated concentration of iron in the deep Archean − than in aquatic samples, especially under aerobic conditions, ocean,99 104 ﬂux of elemental sulfur produced by photolysis 30−32,34 due to the higher density of microbial biomass compared to of SO2, and sulfate concentrations in the Archean − − water.92,93 In oxic sediment samples a decrease of NCS seawater,105 107 we have estimated concentration of poly- concentration was observed already in <12 h. In the samples ﬁ 108 − − sul des, the most reactive zerovalent sulfur species in the with an initial NCS concentration ≤300 μmol·L 1, it was − · −1 − Archean ocean to be in the 4 40 nmol L range. Atmospheric completely degraded in 14 days. The rates of NCS reactions between methane and nitrogen compounds are decomposition under various redox conditions change in the ﬁ thought to have led to the formation of free hydrogen same order as in water samples: oxic > sul dic > anoxic > cyanide,29,33 which is a precursor for the formation of ferruginous ≈ manganous (Figure 1c−e). Concentrations of − thiocyanate. Calculated thiocyanate concentration in the nitrate in the anoxic Red Sea sediments are ≤6.5 μmol·L 1 in − Archean ocean based on rates of formation of thiocyanate the upper 5 cm and <1 μmol·L 1 in the deeper sediments.94,95 from polysulﬁde and hydrogen cyanide17 and on rates of its In the anoxic sediments of Lake Kinneret concentrations of ∼ · −1 nitrate are ≤0.01 μmol·L−1.96 decomposition from this work is 6.5 nmol L . An important point to note is that despite the very low concentration of Comparison between Marine and Limnic Systems. In − ﬀ NCS , the entire deposition ﬂux of HCN, which was suggested contrast to water column, the di erences in thiocyanate 109 decomposition rates between limnic and marine sediments to be an important precursor of RNA, proteins, and lipids, is were minor (Figure 2b). The Lake Kinneret sediments are lost via this pathway, with likely implications for estimates of characterized by high concentration of methane (up to 3 mmol· cyanide concentrations.110 Work on a comprehensive model of L−1).96,97 Our results did not demonstrate any eﬀect of the the coupled sulfur and cyanide cycles is ongoing, and will be the presence of methanogenic microorganisms on the rates of topic of a future publication.
1240 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article ■ AUTHOR INFORMATION (16) Kamyshny, A. Improved cyanolysis protocol for detection of zero-valent sulfur in natural aquatic systems. Limnol. Oceanogr.: Corresponding Author Methods 2009, 7, 442−448. *E-mail: [email protected]. (17) Luthy, R. G.; Bruce, S. G., Jr. Kinetics of reaction of cyanide and ORCID reduced sulfur species in aqueous solutions. Environ. Sci. Technol. 1979, 13, 1481−1487. Alexey Kamyshny Jr.: 0000-0002-1053-2858 (18) Dzombak, D. A.; Ghosh, R. S.; Young, T. C. In Cyanide in Water Notes and Soil; Dzombak, D. A., Ghosh, R. S., Wong-Chong, G. M., Eds.; − The authors declare no competing ﬁnancial interest. CRC Press: Boca Raton, 2006; pp 57 92. (19) Kamyshny, A.; Zerkle, A. L.; Mansaray, Z. F.; Ciglenecki,̌ I.; Bura-Nakic,́ E.; Farquhar, J.; Ferdelman, T. G. Biogeochemical sulfur ■ ACKNOWLEDGMENTS cycling in the water column of a shallow stratified sea-water lake: This work was supported by the Marie Curie Actions CIG Speciation and quadruple sulfur isotope composition. Mar. Chem. 2011, 127, 144−154. PCIG10-GA-2011-303740 (ThioCyAnOx) Grant. I.H. ac- (20) Bartlett, P. D.; Davis, R. E. J. Am. Chem. Soc. 1958, 80, 2513. knowledges a Starting Grant (337183) from the European (21) Szekeres, L. Analytical chemistry of sulfur acids. Talanta 1974, Research Council. The authors would like to thank Werner 21,1−44. Eckert, Asaph Rivlin, Valeria Boyko, and Hanni Leibowitz for (22)Gupta,N.;Balomajumder,C.;Agarwal,V.Enzymatic help with natural water and sediment sampling and Orit Sivan mechanism and biochemistry for cyanide degradation: a review. J. and Ariel Kushmaro for help with samples processing and Hazard. Mater. 2010, 176,1−13. analyses. (23) Wong-Chong, G. M.; Dzombak, D. A.; Ghosh, R. S. In Cyanide in Water and Soil; Dzombak, D. A., Ghosh, R. S., Wong-Chong, G. M., Eds.; CRC Press: Boca Raton, 2006; pp 1−14. ■ REFERENCES (24) Ghosh, R. S.; Ebbs, S. D.; Bushey, J. T.; Neuhauser, E. F.; (1) Beekhuis, H. A. Technology and industrial applications. In Wong-Chong, G. M. In Cyanide in Water and Soil; Dzombak, D. A., Chemistry and Biochemistry of Thiocyanic Acid and its Derivatives; Ghosh, R. S., Wong-Chong, G. M., Eds.; CRC Press: Boca Raton, Newman, A. A., Ed.; Academic Press: London, 1975; pp 222−255. 2006; pp 225−236. (2) Neufeld, R. D.; Valiknac, T. Inhibition of phenol degradation by (25) Yu, X.; Zhou, P.; Zhou, X.; Liu, Y. Cyanide removal y Chinese thiocyanate. J. Water Pollut. Control 1979, 51, 2283−2291. vegetationquantification of the Michaelis-Menten kinetics. Environ. (3) Shieh, W. K.; Richards, D. J. Anoxic/oxic activated sludge Sci. Pollut. Res. 2005, 12, 221−226. treatment kinetics of cyanides and phenols. J. Environ. Eng. 1988, 114, (26) Kamyshny, A.; Oduro, H.; Mansaray, Z. F.; Farquhar, J. 639−654. Hydrogen cyanide accumulation and transformations in non-polluted (4) Paruchuri, Y. L.; Shivaraman, N.; Kumaran, P. Microbial salt marsh sediments. Aquat. Geochem. 2013, 19,97−113. transformation of thiocyanate. Environ. Pollut. 1990, 68,15−28. (27) Dowler, M. J.; Ingmanson, D. E. Thiocyanate in Red Sea brine (5) Wood, P. A.; Kelly, P. D.; McDonald, R. I.; Jordan, L. S.; Morgan, and its implications. Nature 1979, 279,51−52. D. T.; Khan, S.; Murrell, J. C.; Borodina, E. A novel pink-pigmented (28) Kamyshny, A.; Druschel, G.; Mansaray, Z. F.; Farquhar, J. facultative methylotroph, Methylobacterium thiocyanatum sp. nov., Multiple sulfur isotopes fractionation evidence of abiotic sulfur capable of growth of thiocyanate or cyanate as sole nitrogen sources. transformations in Yellowstone National Park geothermal springs. Arch. Microbiol. 1998, 169, 148−221. Geochem. Trans. 2014, 15,7. (6) Chakraborty, S.; Veeramani, H. Response of pulse phenol (29) Zahnle, K. J. Photochemistry of methane and the formation of injection on an anaerobic−anoxic−aerobic system. Bioresour. Technol. hydrocyanic acid (HCN) in the Earth’s early atmosphere. J. Geophys. 2005, 96, 761−767. Res. 1986, 91, 2819−2834. (7) Boucabeille, C.; Bories, A.; Ollivier, P.; Michel, G. Microbial (30) Pavlov, A. A.; Kasting, J. F. Mass-independent fractionation of degradation of metal complexed cyanides and thiocyanate from mining sulfur isotopes in Archean sediments: Strong evidence for an anoxic wastewaters. Environ. Pollut. 1994, 84,59−67. Archean atmosphere. Astrobiology 2002, 2,27−41. (8) Watts, M. P.; Gan, H. M.; Peng, L. Y.; LêCao, K.-A.; Moreau, J. (31) Ono, S.; Eigenbrode, J. L.; Pavlov, A. A.; Kharecha, P.; Rumble, W. In situ stimulation of thiocyanate biodegradation through D., III; Kasting, J. F.; Freeman, K. H. New insights into Archean sulfur phosphate amendment in gold mine tailings water. Environ. Sci. cycle from mass-independent sulfur isotope records from the Technol. 2017, 51, 13353−13362. Hamersley Basin, Australia. Earth Planet. Sci. Lett. 2003, 213,15−30. (9) Patil, Y. B. Utilization of thiocyanate (SCN−) by a metabolically (32) Zahnle, K.; Claire, M.; Catling, D. The loss of mass-independent active bacterial consortium as the sole source of nitrogen. Int. J. Chem., fractionation is sulfur due to a Palaeoproterozoic collapse of Environ. Pharm. Res. 2011, 2,44−48. atmospheric methane. Geobiology 2006, 4, 271−283. (10) Douglas Gould, W.; King, M.; Mohapatra, B. R.; Cameron, R. (33) Tian, F.; Kasting, J. F.; Zahnle, K. Revisiting HCN formation in A.; Kapoor, A.; Koren, D. W. A critical review on destruction of Earth’s early atmosphere. Earth Planet. Sci. Lett. 2011, 308, 417−423. thiocyanate in mining effluents. Miner. Eng. 2012, 34,38−47. (34) Claire, M.; Kasting, J.; Domagal-Goldman, S.; Stüeken, E.; (11) Brown, P. D.; Morra, M. J. Fate of ionic thiocyanate (SCN−)in Buick, R.; Meadows, V. Modeling the Signature of Sulfur Mass- soil. J. Agric. Food Chem. 1993, 41, 978−982. Independent Fractionation produced in the Archean Atmosphere. (12) Kelly, D. P.; Malin, G.; Wood, A. P. Microbial transformations Geochim. Cosmochim. Acta 2014, 141, 365−380. and biogeochemical cycling of one-carbon substrates containing (35) Sorokin, D. Y. Biological oxidation of sulfur atom in C1 and sulphur, nitrogen or halogens. In Microbial Growth on C1 Compounds; organic compounds. Microbiology (Engl. Transl. Mikrobiologiya) 1993, Murrell, J. C., Kelly, D. P., Eds; Intercept: Andover, 1993; pp 47−63. 62, 575−581. (13) Halkier, B. A.; Gershenzon, J. Biology and biochemistry of (36) Jeong, Y.-S.; Chung, J. S. Biodegradation of thiocyanate in glucosinolates. Annu. Rev. Plant Biol. 2006, 57, 303−333. biofilm reactor using fluidized-carriers. Process Biochem. 2006, 41, (14) Sirikantaramas, S.; Yamazaki, M.; Saito, K. Mechanisms of 701−707. resistance to self-produced toxic secondary metabolites in plants. (37) Chaudhari, A. U.; Kodam, K. M. Biodegradation of thiocyanate Phytochem. Rev. 2008, 7, 467−477. using co-culture of Klebsiella pneumoniae and Ralstonia sp. Appl. (15) Ezzi, M. I.; Pascual, J. A.; Gould, B. J.; Lynch, J. M. Microbiol. Biotechnol. 2010, 85, 1167−1174. Characterisation of the rhodanese enzyme in Trichoderma spp. Enzyme (38) Berben, T.; Overmars, L.; Sorokin, D. Y.; Muyzer, G. Microb. Technol. 2003, 32, 629−634. Comparative genome analysis of three thiocyanate oxidizing
1241 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
Thioalkalivibrio species isolated from soda lakes. Front. Microbiol. 2017, (59) de Kruyff, C. D.; van der Walt, J. I.; Schwartz, H. M. The 8, 254. utilization of thiocyanate and nitrate by thiobacilli. Antonie van (39) Happold, F. C.; Jones, G. L.; Pratt, D. B. Utilization of Leeuwenhoek 1957, 23, 305−316. thiocyanate by Thiobacillus thioparus and T. thiocyanoxidans. Nature (60) Anderson, P. M.; Little, R. M. Kinetic properties of cyanase. 1958, 182, 266−267. Biochemistry 1986, 25, 1621−1626. (40) Hutchinson, M.; Johnstone, K. I.; White, D. The taxonomy of (61) Kunz, D. A.; Nagappan, O. Appl. Environ. Microbiol. 1989, 55, certain thiobacilli. J. Gen. Microbiol. 1965, 41, 357−366. 256−258. (41) Katayama, Y.; Kuraishi, H. Characteristics of Thiobacillus (62) Anderson, P. M.; Sung, Y.-C.; Fuchs, J. A. The cyanase operon thiopaurs and its thiocyanate assimilation. Can. J. Microbiol. 1978, 24, and cyanate metabolism. FEMS Microbiol. Lett. 1990, 87, 247−252. 804−810. (63) Katayama, Y.; Narahara, Y.; Inoue, Y.; Amano, F.; Kanagawa, T.; (42) Kelly, D. P.; Baker, S. C. The organosulphur cycle: aerobic and Kuraishi, H. A. thiocyanate hydrolase of Thiobacillus thioparus. A novel anaerobic processes leading to turnover of C1-sulfur compounds. enzyme catalyzing the formation of carbonyl sulfide from thiocyanate. FEMS Microbiol. Lett. 1990, 87, 241−246. J. Biol. Chem. 1992, 267, 9170−9175. (43) Watts, M. P.; Spurr, L. P.; Gan, H. M.; Moreau, J. W. (64) Kim, S.-J.; Katayama, Y. Effect of growth conditions on Characterization of an autotrophic bioreactor microbial consortium thiocyanate degradation and emission of carbonyl sulfide by − degrading thiocyanate. Appl. Microbiol. Biotechnol. 2017, 101, 5889 Thiobacillus thioparus THI 115. Water Res. 2000, 34, 2887−2894. 5901. (65) Smith, N. A.; Kelly, D. P. Oxidation of carbon disulphide as the (44) Sorokin, D. Y.; Abbas, B.; van Zessen, E.; Muyzer, G. Isolation sole source of energy for the autotrophic growth of Thiobacillus and characterization of an obligately chemolithoautotrophic Halothio- thioparus strain TK-m. Microbiology 1988, 134, 3041−3048. bacillus strain capable of growth on thiocyanate as an energy source. (66) Katayama, Y.; Hiraishi, A.; Kuraishi, H. Paracoccus thiocyanatus − FEMS Microbiol. Lett. 2014, 354,69 74. sp. nov., a new species of thiocyanate-utilizing facultative chemo- (45) Bezsudnova, E. Y.; Sorokin, D. Y.; Tikhonova, T. V.; Popov, V. lithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus O. Thiocyanate hydrolase, the primary enzyme initiating thiocyanate as Paracoccus versutus comb. nov. with emendation of the genus. degradation in the novel obligately chemolithoautotrophic halophilic Microbiology 1995, 141, 1469−1477. sulfur-oxidizing bacterium Thiohalophilus thiocyanoxidans. Biochim. (67) Yamasaki, M.; Matsushita, Y.; Namura, M.; Nyunoya, H.; − Biophys. Acta, Proteins Proteomics 2007, 1774, 1563 1570. Katayama, Y. Genetic and immunochemical characterization of (46) Stafford, D. A.; Callely, A. G. The utilization of thiocyanate by a thiocyanate-degrading bacteria in lake water. Appl. Environ. Microbiol. heterotrophic bacterium. J. Gen. Microbiol. 1969, 55, 285−289. − ’ ’ 2002, 68, 942 946. (47) Grigor eva, N. V.; Avakyan, Z. A.; Tourova, T. P.; Kondrat eva, (68) Sorokin, D. Y.; Tourova, T. P.; Lysenko, A. M.; Mityushina, L. T. F.; Karavaiko, G. I. The search for and study of microorganisms that − L.; Kuenen, J. G. Thioalkalivibrio thiocyanoxidans sp. nov. and degrade cyanides and thiocyanates. Microbiology 1999, 68, 453 460. Thioalkalivibrio paradoxus sp. nov., novel alkaliphilic, obligately (48) Betts, P. M.; Rinder, D. F.; Fleeker, J. R. Thiocyanate utilization autotrophic, sulfur-oxidizing bacteria capable of growth on thiocyanate, by an Arthrobacter. Can. J. Microbiol. 1979, 25, 1277−1282. from soda lakes. Int. J. Syst. Evol. Microbiol. 2002, 52, 657−664. (49) Ghosh, W.; Roy, P. Chemolithoautotrophic oxidation of (69) Sorokin, D. Y.; Antipov, A. N.; Muyzer, G.; Kuenen, J. G. thiosulphate, tetrathionate and thiocyanate by a novel rhizobacterium Anaerobic growth of the haloalkaliphilic denitrifying sulfur-oxidizing belonging to the genus Paracoccus. FEMS Microbiol. Lett. 2007, 270, bacterium Thialkalivibrio thiocyanodenitrificans sp.nov.with thiocya- 124−131. 2004 − (50) Vu, H. P.; Mu, A.; Moreau, J. W. Biodegradation of thiocyanate nate. Microbiology , 150, 2435 2442. (70) Stratford, J.; Dias, A. E.; Knowles, C. J. The utilization of by a novel strain of Burkholderia phytofirmans from soil contaminated by gold mine tailings. Lett. Appl. Microbiol. 2013, 57 (4), 368−372. thiocyanate as a nitrogen source by a heterotrophic bacterium: the (51) Vu, H. P.; Moreau, J. W. Moreau Effects of environmental degradative pathway involves formation of ammonia and tetrathionate. Microbiology 1994, 140, 2657−2662. parameters on thiocyanate biodegradation by Burkholderia phytofir- ’ ’ ’ mans candidate strain ST01hv. Environ. Eng. Sci. 2017, DOI: 10.1089/ (71) Grigor eva, N. V.; Kondrat eva, T. F.; Krasil nikova, E. N.; ees.2016.0351. Karavaiko, G. I. Mechanism of cyanide and thiocyanate decomposition (52) Mason, F.; Harper, D.; Larkin, M. The microbial degradation of by an association of Pseudomonas putida and Pseudomonas stutzeri − thiocyanate. Biochem. Soc. Trans. 1994, 22, 423S. strains. Microbiology 2006, 75, 266 273. (53) Grigor’eva, N. V.; Smirnova, Yu.V.; Terekhova, S. V.; Karavaiko, (72) Hung, C.; Pavlostathis, S. G. Aerobic biodegradation of − G. I. Isolation of an aboriginal bacterial community capable of utilizing thiocyanate. Water Res. 1997, 31, 2761 70. cyanide, thiocyanate and ammonia from metallurgical plant waste- (73) Hung, C.; Pavlostathis, S. G. Kinetics and modeling of water. Appl. Biochem. Microbiol. 2008, 44, 502−506. autotrophic thiocyanate biodegradation. Biotechnol. Bioeng. 1999, 62, − (54) Sorokin, D. Y.; Tourova, T. P.; Lysenko, A. M.; Kuenen, J. G. 1 11. Microbial thiocyanate utilization under highly alkaline conditions. (74) du Plessis, C. A.; Barnard, P.; Muhlbauer, R. M.; Naldrett, K. Appl. Environ. Microbiol. 2001, 67, 528−538. Empirical model for the autotrophic biodegradation of thiocyanate in − (55) Sorokin, D. Y.; Tourova, T. P.; Bezsoudnova, E. Y.; Pol, A.; an activated sludge reactor. Lett. Appl. Microbiol. 2001, 32, 103 107. Muyzer, G. Denitrification in a binary culture and thiocyanate (75) Lee, C.; Kim, J.; Do, H.; Hwang, S. Monitoring thiocyanate- metabolism in Thiohalophilus thiocyanooxidans gen. nov. sp. nov. − a degrading microbial community in relation to changes in process moderately halophilic chemolithotrophic sulfur-oxidizing gammapro- performance in mixed culture systems near washout. Water Res. 2008, teobacterium from hypersaline lakes. Arch. Microbiol. 2007, 187, 441− 42, 1254−1262. 450. (76) Lay-Son, M.; Drakides, C. New approach to optimize (56) Sorokin, D. Y.; Kovaleva, O. L.; Tourova, T. P.; Muyzer, G. operational conditions for the biological treatment of a high-strength Thiohalobacter thiocyanaticus gen. nov. sp. nov., a moderately thiocyanate and ammonium waste: pH as key factor. Water Res. 2008, halophilic, sulfur-oxidizing Gammaproteobacterium from hypersaline 42 (3), 774−780. lakes, that utilizes thiocyanate. Int. J. Syst. Evol. Microbiol. 2010, 60, (77) Kantor, R. S.; van Zyl, A. W.; van Hille, R. P.; Thomas, B. C.; 444−450. Harrison, S. T.; Banfield, J. F. Bioreactor microbial ecosystems for (57) Happold, F. C.; Key, A. The bacterial purification of gasworks thiocyanate and cyanide degradation unravelled with genome-resolved liquors. II. The biological oxidation of ammonium thiocyanate. metagenomics. Environ. Microbiol. 2015, 17, 4929−4941. Biochem. J. 1937, 31, 1323−1329. (78) van Zyl, A. W.; Huddy, R.; Harrison, S. T. L.; van Hille, R. P. (58) Youatt, J. B. Studies on the metabolism of Thiobacillus Evaluation of the ASTER process in the presence of suspended solids. thiocyanoxidans. J. Gen. Microbiol. 1954, 11, 139−149. Miner. Eng. 2015, 76, 72.
1242 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243 Environmental Science & Technology Article
(79) Ahn, J. H.; Kim, J.; Lim, J.; Hwang, S. Biokinetic evaluation and (103) Raiswell, R.; Reinhard, C. T.; Derkowski, A.; Owens, J.; modeling of continuous thiocyanate biodegradation by Klebsiella sp. Bottrell, S. H.; Anbar, A. D.; Lyons, T. W. Formation of syngenetic Biotechnol. Prog. 2004, 20, 1069−1075. and early diagenetic iron minerals in the late Archean Mt. McRae (80) Hung, C.-H.; Pavlostathis, S. G. Fate and transformation of Shale, Hamersley Basin, Australia: new insights on the patterns, thiocyanate and cyanate under methanogenic conditions. Appl. controls and paleoenvironmental implications of authigenic mineral Microbiol. Biotechnol. 1998, 49, 112−116. formation. Geochim. Cosmochim. Acta 2011, 75, 1072−1087. (81) Sahariah, B. P.; Chakraborty, S. Kinetic analysis of phenol, (104) Halevy, I.; Alesker, M.; Schuster, E. M.; Popovitz-Biro, R.; thiocyanate and ammonia-nitrogen removals in an anaerobic−anoxic− Feldman, Y. A key role for green rust in the Precambrian oceans and aerobic moving bed bioreactor system. J. Hazard. Mater. 2011, 190, the genesis of iron formations. Nat. Geosci. 2017, 10, 135−139. 260−267. (105) Jamieson, J. W.; Wing, B. A.; Farquhar, J.; Hannington, M. D. (82) Rong, L.; Lim, L.; Takeuchi, T. Determination of iodide and Neoarchaean seawater sulphate concentrations from sulphur isotopes thiocyanate in seawater by liquid chromatography with poly(ethylene in massive sulphide ore. Nat. Geosci. 2013, 6,61−63. glycol) stationary phase. Chromatographia 2005, 61, 371−374. (106) Halevy, I. Production, preservation and biological processing of (83) Cline, J. Spectrophotometric determination of hydrogen sulfide mass-independent sulfur isotope fractionation in the Archean surface in natural waters. Limnol. Oceanogr. 1969, 14, 454−458. environment. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 17644−17649. (84) Stookey, L. L. Ferrozine − a new spectrophotometric reagent (107) Crowe, S. A.; Paris, G.; Katsev, S.; Jones, C.; Kim, S.-T.; Zerkle, for iron. Anal. Chem. 1970, 42, 779−782. A. L.; Nomosatryo, S.; Fowle, D. A.; Adkins, J. F.; Sessions, A. L.; (85) Goto, K.; Taguchi, S.; Fukue, Y.; Ohta, K.; Watanabe, H. Farquhar, J.; Canfield, D. E. Sulfate was a trace constituent of Archean Spectrophotometric determination of manganese with 1-(2-pyridyla- seawater. Science 2014, 346, 735−737. zo)-2-naphthol and a non-ionic surfactant. Talanta 1977, 24, 752− (108) Kamyshny, A.; Gun, J.; Rizkov, D.; Voitsekovski, T.; Lev, O. 753. Equilibrium distribution of polysulfide ions in aqueous solutions at ﬁ (86) FireSting O2 Manual, 2015. www.pyroscience.com/media/ les/ different temperatures by rapid single phase derivatization. Environ. Sci. Manual%20Firesting%20O2.pdf. Technol. 2007, 41, 2395−2400. (87) Michaelis, L.; Menten, M. Die Kinetik der Invertinwirkung. (109) Patel, B. H.; Percivalle, C.; Ritson, D. J.; Duffy, C. D.; Biochem. Z. 1913, 49, 333−369. Sutherland, J. D. Common origins of RNA, protein and lipid (88) Boethling, R. S.; Alexander, M. Effect of concentration of precursors in a cyanosulfidic protometabolis. Nat. Chem. 2015, 7 − organic chemicals on their biodegradation by natural microbial (4), 301 307. communities. Appl. Environ. Microbiol. 1979, 37, 1211−1216. (110) Stribling, R.; Miller, S. L. Energy yields for hydrogen cyanide (89) Jørgensen, B. B. Bacteria and marine biogeochemistry. In Marine and formaldehyde syntheses: the HCN and amino acid concentrations − Geochemistry; Schulz, H. D., Zabel, M., Eds.; Springer-Verlag: Berlin, in the primitive ocean. Origins Life Evol. Biospheres 1987, 17, 261 273. 2000; pp 173−207. (90) Nishri, A. Quantitative aspects of the nitrogen cycle. In Lake KinneretEcology and Management; Zohary, T., Sukenik, A., Berman, T.; Nishri, A., Eds.; Springer, 2014; pp 365−381. (91) Bahta, A.; Parker, G. A.; Tuck, D. Critical survey of stability constants of complexes thiocyanate ion. Pure Appl. Chem. 1997, 69, 1489−1548. (92) Kallmeyer, J.; Pockalny, R.; Adhikari, R. R.; Smith, D. C.; D’Hondt, S. Global distribution of microbial abundance and biomass in subseafloor sediment. Proc. Natl. Acad. Sci. U. S. A. 2012, 109 (40), 16213−16216. (93) Schwarz, J.; Eckert, W.; Conrad, R. Community structure of archaea and bacteria in a profundal lake sediment Lake Kinneret (Israel). Syst. Appl. Microbiol. 2007, 30, 239−254. (94) Al-Rousan, S.; Rasheed, M.; Badran, M. Nutrient diffusive fluxes from sediments in the northern Gulf of Aqaba, Red Sea. Sci. Mar. 2004, 68, 483−490. (95) Rasheed, M.; Al-Rousan, S.; Manasrah, R.; Al-Horani, F. Nutrient fluxes from deep sediment support nutrient budget in the oligotrophic waters of the Gulf of Aqaba. J. Oceanogr. 2006, 62,83−89. (96) Sivan, O.; Adler, M.; Pearson, A.; Gelman, F.; Bar-Or, I.; John, S. G.; Eckert, W. Geochemical evidence for iron-mediated anaerobic oxidation of methane. Limnol. Oceanogr. 2011, 56, 1536−1544. (97)Adler,M.;Eckert,W.;Sivan,O.Quantifyingratesof methanogenesis and methanotrophy in Lake Kinneret sediments (Israel) using pore-water profiles. Limnol. Oceanogr. 2011, 56, 1525− 1535. (98) Poulton, S. W.; Canfield, D. Ferruginous conditions: A dominant feature of the ocean through Earth’s history. Elements 2011, 7, 107−112. (99) Holland, H. D. The Chemical Evolution of the Atmosphere and Oceans; Wiley-Interscience: New York, 1984; p. 351. (100) Grotzinger, J. P.; Kasting, J. F. New constrains on Precambrian ocean composition. J. Geol. 1993, 101, 235−243. (101) Holland, H. D. Volcanic gases, black smokers, and the Great Oxygen Event. Geochim. Cosmochim. Acta 2002, 66, 3811−3826. (102) Canfield, D. E. The early history of atmospheric oxygen: homage to Robert M. Garrels. Annu. Rev. Earth Planet. Sci. 2005, 33, 1−36.
1243 DOI: 10.1021/acs.est.7b04723 Environ. Sci. Technol. 2018, 52, 1234−1243