DOCSLIB.ORG

LABORATORY - TESTING for the Time Period : 10/01/16 and 09/30/2017

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
LABORATORY - TESTING for the Time Period : 10/01/16 and 09/30/2017 LABORATORY - TESTING For the Time Period : 10/01/16 and 09/30/2017 IF YOU ARE COVERED BY HEALTH INSURANCE , YOU ARE STRONGLY ENCOURAGED TO CONSULT WITH YOUR HEALTH INSURER TO DETERMINE ACCURATE INFORMATION ABOUT YOUR FINACIAL RESPONSIBILITY FOR A PARTICULAR HEALTH CARE SERVICE PROVIDED AT THIS HEALTHCARE FACILITY. IF YOU ARE NOT COVERED BY HEALTH INSURANCE YOU ARE STRONGLY ENCOURAGED TO CONTACT THE BUSINESS OFFICE AT (719)584-4508 OR TOLL FREE AT 800-543-4046 TO DISCUSS PAYMENT OPTIONS PRIOR TO RECEIVING A HEALTH CARE SERVICE FROM THIS HEALTH CARE FACILITY SINCE POSTED HEALTHCARE SERVICES MAY NOT REFLECT THE ACTUAL AMOUNT OF YOUR FINANCIAL RESPONSIBILITY. Note: 1. The pricing on this page is for Laboratory testing only. It is not combined pricing with other testing 2. Charging is based on the Length of Stay, amount of supplies used, therapies provided, testing given as well as other care provided 3. This pricing is an average charge and not intended to be the exact charge for any particular patient 4. The average charge shown is an estimate and that actual charges for the service depend on the circumstances at the time the service is provided and the patient. 5. Any discount is negotiated by the insurance provider. Most insurance providers should be able to tell their members what financial responsibility they will have. 6. Patients without insurance are able to receive a discount equal to the insurance provider with the lowest negotiated discount CPT Description Average Charge 80048 METABOLIC PANEL TOTAL CA $ 1,198.36 80050 GENERAL HEALTH PANEL $ 7,521.00 80051 ELECTROLYTE PANEL $ 471.35 80053 COMPREHEN METABOLIC PANEL $ 1,832.37 80055 OBSTETRIC PANEL $ 1,900.00 80061 LIPID PANEL $ 543.48 80069 RENAL FUNCTION PANEL $ 1,349.52 80074 ACUTE HEPATITIS PANEL $ 2,205.10 80076 HEPATIC FUNCTION PANEL $ 872.14 80156 ASSAY CARBAMAZEPINE TOTAL $ 587.98 80158 DRUG ASSAY CYCLOSPORINE $ 50.00 80159 DRUG ASSAY CLOZAPINE $ 115.50 80162 ASSAY OF DIGOXIN TOTAL $ 395.41 80164 ASSAY DIPROPYLACETIC ACID TOTAL $ 524.28 80169 DRUG ASSAY EVEROLIMUS $ 113.75 80170 ASSAY OF GENTAMICIN $ 1,610.40 80175 DRUG SCREEN QUANTITATIVE LAMOTRIGINE $ 100.22 80177 DRUG SCREEN QUANTIOTATIVE LEVETIRACETAM $ 57.16 80178 ASSAY OF LITHIUM $ 266.08 80183 DRUG SCREEN QUANTITATIVE OXCARBAZEPIN $ 60.00 80184 ASSAY OF PHENOBARBITAL $ 452.08 80185 ASSAY OF PHENYTOIN TOTAL $ 647.72 80186 ASSAY OF PHENYTOIN FREE $ 28.00 80195 ASSAY OF SIROLIMUS $ 97.00 80197 ASSAY OF TACROLIMUS $ 115.21 80198 ASSAY OF THEOPHYLLINE $ 537.00 80201 ASSAY OF TOPIRAMATE $ 46.00 80202 ASSAY OF VANCOMYCIN $ 672.78 80203 DRUG SCREEN QUANTITATIVE ZONISAMIDE $ 87.00 80299 QUANTITATIVE ASSAY DRUG $ 180.00 81001 URINALYSIS AUTO W/SCOPE $ 291.83 81003 URINALYSIS AUTO W/OUT SCOPE $ 223.41 81025 URINE PREGNANCY TEST $ 358.48 81050 URINALYSIS VOLUME MEASURE $ 187.80 81221 CFTR(CYSTIC FUBROSIS TRANSMEMBRANE) GENE KNOWN FAM VARIANTS $ 225.00 81235 EGFR GENE COM VARIANTS $ 992.25 81240 F2 GENE $ 192.40 81241 F5 GENE $ 262.44 81243 FMR1 GENE DETECTION $ 1,173.00 81270 JAK2 GENE $ 339.50 81332 SERPINA1 GENE $ 336.00 81374 HLA I TYPING 1 ANTIGEN LR $ 191.25 82010 ACETONE ASSAY $ 65.12 82017 ACYLCARNITINES QUANT $ 23.67 82024 ASSAY OF ACTH (ADRENOCORTICOTROPIC) $ 79.57 82040 ASSAY OF SERUM ALBUMIN $ 164.64 82042 ASSAY OF URINE ALBUMIN $ 78.30 82043 MICROALBUMIN QUANTITATIVE $ 20.51 82085 ASSAY OF ALDOLASE $ 32.00 82088 ASSAY OF ALDOSTERONE $ 103.75 82103 ALPHA-1-ANTITRYPSIN TOTAL $ 44.00 82105 ALPHA-FETOPROTEIN SERUM $ 49.00 82139 AMINO ACIDS QUAN 6 OR MORE $ 42.24 82140 ASSAY OF AMMONIA $ 932.66 82150 ASSAY OF AMYLASE $ 453.62 82164 ANGIOTENSIN I ENZYME TEST $ 34.00 82172 ASSAY OF APOLIPOPROTEIN $ 57.00 82175 ASSAY OF ARSENIC $ 67.67 82232 ASSAY OF BETA-2 PROTEIN $ 44.00 82247 BILIRUBIN TOTAL $ 183.98 82248 BILIRUBIN DIRECT $ 176.74 82270 OCCULT BLOOD FECES $ 29.00 82271 OCCULT BLOOD OTHER SOURCES $ 17.00 82272 OCCULT BLOOD FECES 1-3 TESTS $ 19.01 82306 VITAMIN D 25 HYDROXY $ 250.00 82308 ASSAY OF CALCITONIN $ 63.00 82310 ASSAY OF CALCIUM $ 209.52 82330 ASSAY OF CALCIUM $ 170.44 82340 ASSAY OF CALCIUM IN URINE $ 220.40 82365 CALCULUS SPECTROSCOPY $ 41.84 82375 ASSAY CARBOXYHB QUANT $ 420.54 82378 CARCINOEMBRYONIC ANTIGEN $ 237.84 82379 ASSAY OF CARNITINE $ 24.32 82382 ASSAY URINE CATECHOLAMINES $ 35.00 82384 ASSAY THREE CATECHOLAMINES $ 84.00 82390 ASSAY OF CERULOPLASMIN $ 29.00 82397 CHEMILUMINESCENT ASSAY $ 128.00 82436 ASSAY OF URINE CHLORIDE $ 231.20 82465 ASSAY BLD/SERUM CHOLESTEROL $ 121.13 82507 ASSAY OF CITRATE $ 197.00 82525 ASSAY OF COPPER $ 29.00 82530 CORTISOL FREE $ 55.00 82533 TOTAL CORTISOL $ 2,382.34 82550 ASSAY OF CK (CPK) $ 195.24 82553 CREATINE MB FRACTION $ 731.08 82565 ASSAY OF CREATININE $ 130.63 82570 ASSAY OF URINE CREATININE $ 285.21 82575 CREATININE CLEARANCE TEST $ 291.67 82595 ASSAY OF CRYOGLOBULIN $ 23.00 82607 VITAMIN B-12 $ 577.90 82626 DEHYDROEPIANDROSTERONE $ 67.00 82627 DEHYDROEPIANDROSTERONE $ 75.00 82652 VIT D 1 25-DIHYDROXY $ 123.53 82657 ENZYME CELL ACTIVITY $ 245.00 82668 ASSAY OF ERYTHROPOIETIN $ 55.00 82670 ASSAY OF ESTRADIOL $ 57.00 82693 ASSAY OF ETHYLENE GLYCOL $ 152.00 82705 FATS/LIPIDS FECES QUALITATIVE $ 19.33 82728 ASSAY OF FERRITIN $ 600.03 82731 ASSAY OF FETAL FIBRONECTIN $ 776.95 82746 ASSAY OF FOLIC ACID SERUM $ 501.13 82784 ASSAY IGA(IMMUNOGLOBULIN/IGD(IMMUNOGLOBULIN D)/IGG(IMMUNGLOBULIN GAMMA)/IGM(IMMUNOGLOBULIIN$ 524.09 M ANTIBODIES) EACH 82785 ASSAY OF IGE $ 47.81 82787 IGG 1 2 3 OR 4 EACH $ 34.00 82800 BLOOD PH $ 488.18 82805 BLOOD GASES W/O2 SATURATION $ 741.23 82941 ASSAY OF GASTRIN $ 36.00 82945 GLUCOSE OTHER FLUID $ 258.12 82947 ASSAY GLUCOSE BLOOD QUANT $ 164.90 82950 GLUCOSE TEST $ 202.00 82951 GLUCOSE TOLERANCE TEST (GTT) $ 424.00 82952 GTT-ADDED SAMPLES $ 357.00 82955 ASSAY OF G6PD ENZYME $ 74.00 82962 GLUCOSE BLOOD TEST $ 308.89 82977 ASSAY OF GGT(GAMMA-GLUTAMYL TRANSPEPTIDASE $ 169.55 83001 ASSAY OF GONADOTROPIN (FSH) $ 38.11 83002 ASSAY OF GONADOTROPIN (LH) $ 38.13 83003 ASSAY GROWTH HORMONE (HGH) $ 102.00 83010 ASSAY OF HAPTOGLOBIN QUANT $ 312.87 83021 HEMOGLOBIN CHROMOTOGRAPHY $ 138.37 83036 GLYCOSYLATED HEMOGLOBIN TEST $ 57.52 83050 BLOOD METHEMOGLOBIN ASSAY $ 975.00 83090 ASSAY OF HOMOCYSTINE $ 174.54 83497 ASSAY OF 5-HIAA (HYDROXYINDOLEACETIC ACID) $ 42.00 83498 ASSAY OF PROGESTERONE 17-D $ 76.00 83516 IMMUNOASSAY NONANTIBODY $ 86.00 83519 RIA NONANTIBODY $ 100.02 83520 IMMUNOASSAY QUANT NOS NONAB $ 111.59 83525 ASSAY OF INSULIN $ 36.30 83540 ASSAY OF IRON $ 446.12 83550 IRON BINDING TEST $ 358.04 83605 ASSAY OF LACTIC ACID $ 244.99 83615 LACTATE (LD - LIPID DROPLETS) (LDH -LACTIC ACID DEHYDROGENASE) ENZYME $ 169.55 83631 LACTOFERRIN FECAL (QUANTITATIVE) $ 170.00 83655 ASSAY OF LEAD $ 28.84 83664 LAMELLAR BODY FETAL LUNG $ 228.00 83690 ASSAY OF LIPASE $ 506.65 83695 ASSAY OF LIPOPROTEIN(A) $ 29.00 83735 ASSAY OF MAGNESIUM $ 462.05 83825 ASSAY OF MERCURY $ 41.67 83835 ASSAY OF METANEPHRINES $ 135.36 83864 MUCOPOLYSACCHARIDES $ 129.00 83873 ASSAY OF CSF PROTEIN $ 35.00 83874 ASSAY OF MYOGLOBIN $ 42.25 83880 ASSAY OF NATRIURETIC PEPTIDE $ 219.69 83883 ASSAY NEPHELOMETRY NOT SPEC $ 46.52 83915 ASSAY OF NUCLEOTIDASE $ 49.00 83916 OLIGOCLONAL BANDS $ 41.00 83919 ORGANIC ACIDS QUAL EACH $ 241.09 83921 ORGANIC ACID SINGLE QUANT $ 174.00 83930 ASSAY OF BLOOD OSMOLALITY $ 216.94 83935 ASSAY OF URINE OSMOLALITY $ 236.61 83945 ASSAY OF OXALATE $ 33.00 83970 ASSAY OF PARATHORMONE $ 153.63 83993 ASSAY FOR CALPROTECTIN FECAL $ 231.25 84030 ASSAY OF BLOOD PKU(PHENYLKETONURIA) $ 613.00 84075 ASSAY ALKALINE PHOSPHATASE $ 122.62 84080 ASSAY ALKALINE PHOSPHATASES $ 50.00 84100 ASSAY OF PHOSPHORUS $ 147.97 84112 EVAL AMNIOTIC FLUID PROTEIN $ 102.08 84132 ASSAY OF SERUM POTASSIUM $ 221.71 84133 ASSAY OF URINE POTASSIUM $ 241.55 84134 ASSAY OF PREALBUMIN $ 270.50 84144 ASSAY OF PROGESTERONE $ 49.19 84145 PROCALCITONIN (PCT) $ 304.12 84146 ASSAY OF PROLACTIN $ 46.12 84153 ASSAY OF PSA TOTAL $ 215.15 84155 ASSAY OF PROTEIN SERUM $ 126.11 84156 ASSAY OF PROTEIN URINE $ 296.42 84157 ASSAY OF PROTEIN OTHER $ 292.55 84165 PROTEIN E-PHORESIS SERUM $ 235.33 84210 ASSAY OF PYRUVATE $ 169.42 84244 ASSAY OF RENIN $ 55.00 84260 ASSAY OF SEROTONIN $ 108.00 84295 ASSAY OF SERUM SODIUM $ 125.46 84300 ASSAY OF URINE SODIUM $ 213.60 84305 ASSAY OF SOMATOMEDIN $ 63.57 84311 SPECTROPHOTOMETRY $ 53.50 84402 ASSAY OF FREE TESTOSTERONE $ 52.00 84403 ASSAY OF TOTAL TESTOSTERONE $ 54.51 84425 ASSAY OF VITAMIN B-1 $ 178.00 84432 ASSAY OF THYROGLOBULIN $ 281.59 84436 ASSAY OF TOTAL THYROXINE $ 359.53 84439 ASSAY OF FREE THYROXINE $ 258.94 84443 ASSAY THYROID STIM HORMONE $ 834.50 84445 ASSAY OF TSI(THYROID STIMULATING IMMUNE) GLOBULIN $ 140.00 84446 ASSAY OF VITAMIN E $ 29.00 84450 TRANSFERASE (AST) (SGOT) $ 149.53 84460 ALANINE AMINO (ALT) (SGPT) $ 130.30 84466 ASSAY OF TRANSFERRIN $ 28.67 84478 ASSAY OF TRIGLYCERIDES $ 123.81 84479 ASSAY OF THYROID (T3 OR T4) $ 356.45 84480 ASSAY TRIIODOTHYRONINE (T3) $ 46.00 84481 FREE ASSAY (FT-3) $ 50.00 84482 T3 REVERSE $ 45.00 84484 ASSAY OF TROPONIN QUANT $ 376.19 84520 ASSAY OF UREA NITROGEN $ 125.26 84540 ASSAY OF URINE/UREA-N $ 241.00 84550 ASSAY OF BLOOD/URIC ACID $ 129.83 84560 ASSAY OF URINE/URIC ACID $ 271.50 84585 ASSAY OF URINE VMA(VANILLYLMANDELIC ACID) $ 31.00 84586 ASSAY OF VIP $ 127.00 84588 ASSAY OF VASOPRESSIN $ 105.00 84590 ASSAY OF VITAMIN A $ 54.00 84600 ASSAY OF VOLATILES $ 90.00 84630 ASSAY OF ZINC $ 60.00 84681 ASSAY OF C-PEPTIDE $ 52.00 84702 CHORIONIC GONADOTROPIN TEST $ 576.24 84703 CHORIONIC GONADOTROPIN ASSAY $ 339.55 85008 BLOOD SMEAR W/OUT DIFF WBC(WHITE BLOOD CELL) COUNT $ 48.00 85014 HEMATOCRIT $ 130.47 85018
Load more

Recommended publications
  • Ige – the Main Player of Food Allergy
    DDMOD-431; No of Pages 8 Vol. xxx, No. xx 2016 Drug Discovery Today: Disease Models Editors-in-Chief Jan Tornell – AstraZeneca, Sweden DRUG DISCOVERY Andrew McCulloch – University of California, SanDiego, USA TODAY DISEASE MODELS IgE – the main player of food allergy 1 2,3 2 Henrike C.H. Broekman , Thomas Eiwegger , Julia Upton , 4, Katrine L. Bøgh * 1 Department of Dermatology/Allergology, University Medical Centre Utrecht (UMCU), Utrecht, The Netherlands 2 Division of Immunology and Allergy, Food Allergy and Anaphylaxis Program, The Department of Paediatrics, Hospital for Sick Children, Toronto, Canada 3 Research Institute, Physiology and Experimental Medicine, The University of Toronto, Toronto, Canada 4 National Food Institute, Technical University of Denmark, Søborg, Denmark Food allergy is a growing problem worldwide, presently Section editor: affecting 2–4% of adults and 5–8% of young children. IgE Michelle Epstein – Medical University of Vienna, is a key player in food allergy. Consequently huge Department of Dermatology, DIAID, Experimental Allergy, Waehringer Guertel 18-20, Room 4P9.02, A1090, efforts have been made to develop tests to detect Vienna, Austria. either the presence of IgE molecules, their allergen binding sites or their functionality, in order to provide allergen ingestion [1], and involve one or more of the follow- information regarding the patient’s food allergy. The ing systems; the skin (pruritus, urticaria, or angioedema), the ultimate goal is to develop tools that are capable of gastro-intestinal tract (diarrhea, vomiting, contractions, in- creased bowel movement), the respiratory tract (asthma at- discriminating between asymptomatic sensitization tack, hoarseness, stridor/laryngeal angioedema) or the and a clinically relevant food allergy, and between cardiovascular system (dizziness, drop in blood pressure, loss different allergic phenotypes in an accurate and trust- of consciousness) [2,3].
    [Show full text]
  • Role of Serologic Testing in Rheumatic Diseases
    Role of Serologic Testing in Rheumatic Diseases Debendra Pattanaik MD FACP Associate professor of Medicine UTHSC, Memphis TN Disclosure None Objectives Discuss commonly available serologic testing useful in daily clinical practice Recognize the serologic associations of rheumatic diseases Recognize their diagnostic utilities and limitations Diagnostic Accuracy for Lupus and other autoimmune diseases in the community setting 476 patients were evaluated at Autoimmunity Center of University of Florida, Gainesville for 13 months which were by from primary care physicians SLE was over diagnosed on many patients on the basis of + ANA 39 patients are taking prednisone 60 mg/day who have no autoimmune disease but only have + ANA Inappropriate diagnosis leads to inappropriate therapy, emotional and financial consequences The authors suggested continuing education in screening for autoimmune disease and identify patients who may benefit from early referral. Arch Intern Med. 2004;164:2435-2441 Antinuclear Antibody (ANA) Testing for Connective Tissue Disease British Columbia Population: 4.631 million. More than 94,000 ANA tests were performed in B.C. in fiscal year 2011/12 at a cost of $2.24 million annually. Incidence and Estimated New Cases in B.C. for Selected CTDs Connective Tissue Disease Disease incidence per million population Estimated new BC cases/year * Systemic lupus erythematosus 56 259 Scleroderma 19 88 Dermatomyositis & polymyositis < 10 < 46 Eighteen percent of first-time tested outpatients underwent unnecessary repeat testing in 2010/2011. In 57.2% of the repeat testing, both the initial and the repeat ANA tests were ordered by a GP. In 24.8% the initial test was ordered by a GP and the repeat test was ordered by a specialist, and in 10.2% both the initial and the repeat test were ordered by the same specialist.
    [Show full text]
  • Importance of Ag-Ab Reactions
    Ag-Ab reactions Tests for Ag-Ab reactions EISA SALEHI PhD. Immunology Dept. TUMS Importance of Ag-Ab Reactions • Understand the mechanisms of defense • Abs as tools in: – Treatment – Diagnosis • As biomarkers • As tools to measure analytes Nature of Ag/Ab Reactions http://www.med.sc.edu:85/chime2/lyso-abfr.htm • Lock and Key Concept • Non-covalent Bonds – Hydrogen bonds – Electrostatic bonds – Van der Waal forces – Hydrophobic bonds • Multiple Bonds • Reversible Source: Li, Y., Li, H., Smith-Gill, S. J., Mariuzza, R. A., Biochemistry 39, 6296, 2000 Affinity • Strength of the reaction between a single antigenic determinant and a single Ab combining site High Affinity Low Affinity Ab Ab Ag Ag Affinity = ( attractive and repulsive forces Calculation of Affinity Ag + Ab ↔ Ag-Ab Applying the Law of Mass Action: [[gAg-Ab] Keq = [Ag] x [Ab] Avidity • The overall strength of binding between an Ag with many determinants and multivalent Abs 4 6 10 Keq = 10 10 10 Affinity Avidity Avidity SifiitSpecificity • The ability of an individual antibody combining site to react with only one antigenic determinant. • The ability of a population of antibody molecules to react with only one antigen. Cross Reactivity • The ability of an individual Ab combining site to react with more than one antigenic determinant. • The ability of a population of Ab molecules to react with more than one Ag Cross reactions Anti-A Anti-A Anti-A Ab Ab Ab Ag A Ag B Ag C Shared epitope Similar epitope Factors Affecting Measurement of A/AbRAg/Ab Reac tions • Affinity • Avidity Ab excess Ag excess • AAbiAg:Ab ratio •Phyygsical form of Ag Equivalence – Lattice formation Do you need to know what happens in Lab.
    [Show full text]
  • Principles of Immunochemical Techniques Used in Clinical Laboratories
    Review Received 2.11.06 | Revisions Received 3.1.06 | Accepted 3.2.06 Principles of Immunochemical Techniques Used in Clinical Laboratories Marja E. Koivunen, Richard L. Krogsrud (Antibodies Incorporated, Davis, CA) DOI: 10.1309/MV9RM1FDLWAUWQ3F Abstract binding site. The type of antibody and its diseases. Immunoassays can measure low Immunochemistry offers simple, rapid, robust affinity and avidity for the antigen determines levels of disease biomarkers and therapeutic or yet sensitive, and easily automated methods assay sensitivity and specificity. Depending on illicit drugs in patient’s blood, serum, plasma, for routine analyses in clinical laboratories. the assay format, immunoassays can be urine, or saliva. Immunostaining is an example Immunoassays are based on highly specific qualitative or quantitative. They can be used for of an immunochemical technique, which binding between an antigen and an antibody. the detection of antibodies or antigens specific combined with fluorescent labels allows direct An epitope (immunodeterminant region) on the for bacterial, viral, and parasitic diseases as visualization of target cells and cell structures. antigen surface is recognized by the antibody’s well as for the diagnosis of autoimmune Immunochemistry offers simple, rapid, robust yet sensitive, bind to an antigen. The third domain (complement-binding Fc and in most cases, easily automated methods applicable to routine fragment) forms the base of the Y, and is important in immune analyses in clinical laboratories. Immunochemical methods do not system function and regulation. usually require extensive and destructive sample preparation or The region of an antigen that interacts with an antibody is expensive instrumentation. In fact, most methods are based on called an epitope or an immunodeterminant region.
    [Show full text]
  • Igm Autoantibody Testing by Latex Agglutination Nephelometry and Elisa in Patients with Rheumatoid Arthritis
    European Journal of Molecular & Clinical Medicine ISSN 2515-8260 Volume 07, Issue 08, 2020 Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis Dr.C. Devi1, Dr.R. Ravichandran2, Dr. Logeswari Selvaraj3, Dr.S. Ramesh4, Dr.T. Aarthipriya5 1,2,3,4,5Senior Assistant professor Department of Microbiology, Government Kilpauk Medical College, Chennai mail id: [email protected] ABSTRACT Objectives: To test Rheumatoid factor (RF) IgM autoantibody in Patients with Rheumatoid Arthritis by various methods like latex agglutination, Nephelometer and ELISA. Comparative analysis of the sensitivity and specificity of the tests performed. Materials and Methods: The study was conducted for a period of six months from June 2018 to November 2018 in a tertiary care hospital in Chennai. 90 patients attending Rheumatology OPD or admitted in the ward with the diagnosis of Rheumatoid arthritis, satisfying the inclusion and exclusion criteria were taken up for the study. Inclusion criteria: Clinically diagnosed Rheumatoid Arthritis patient as per revised ACR 1987 classification criteria. Duration of symptoms (1yr- early (RA) 1 Yr. (Established RA). Exclusion Criteria: Those with systemic connective tissue diseases like SLE, Scleroderma, MCTD, Sjogren syndrome, those with chronic liver diseases, tuberculosis, subacute Bacterial endocarditis, Pregnancy, Lympho reticular malignancies are excluded for the study. Those with onset 16 years of age are also excluded. Under aseptic precautions about 3ml of blood was collected from each Patient. Rheumatoid factor (RF) IgM was tested for each patient by all three methods Latex agglutination, Nephelometry and ELISA. Results: IgM rheumatoid factor (RF) was detected in the sera of 90 patients with Rheumatoid Arthritis.
    [Show full text]
  • Prospective Comparison of Laser Nephelometry with Standard Agglutination Techniques for Detection of Rheumatoid Factor
    J Clin Pathol: first published as 10.1136/jcp.40.2.216 on 1 February 1987. Downloaded from J Clin Pathol 1987;40:216-220 Prospective comparison of laser nephelometry with standard agglutination techniques for detection of rheumatoid factor A G PRENTICE,* P HICKLING,t I C WISEMAN,* C J HOLWILL,* J NORTHWOODt From the Departments oftRheumatology, *Haematology, and IMicrobiology, Derriford Hospital, Plymouth, Devon SUMMARY IgM rheumatoid factor was assayed by three routine methods: latex fixation; haem- agglutination; and end point laser nephelometry in 69 patients with definite or classical rheumatoid arthritis and 58 patients with other non-rheumatoid arthropathies, selected prospectively according to the American Rheumatism Association clinical criteria. The operators of the assays were unaware of the clinical diagnoses. In the group with rheumatoid arthritis 75-4% were positive by latex fixation, 73*9% by haemagglutination, and 55 1% by nephelometry. In the group with non- rheumatoid arthropathies 10-4% were positive by latex fixation, 8-6% by haemagglutination, and 10-4% by nephelometry. Thus the simple and inexpensive latex fixation test was as good as the haemagglutination test, and both were significantly better than nephelometry in the laboratory = confirmation of the clinical diagnosis ofdefinite or classic rheumatoid arthritis (X2 5.40 and 4 56,copyright. and p < 0 025 and < 0 05, respectively). None of these tests was significantly better or worse than the others in producing positive results in the group with non-rheumatoid arthropathies. The diagnosis of rheumatoid arthritis is largely based complexes, which scatter a beam of light in propor- on clinical evidence and depends on the fulfilment of tion to the concentration of the antibody in the test the criteria for definite or classic disease, as described serum.
    [Show full text]
  • IMMUNOCHEMICAL TECHNIQUES Antigens Antibodies
    Imunochemical Techniques IMMUNOCHEMICAL TECHNIQUES (by Lenka Fialová, translated by Jan Pláteník a Martin Vejražka) Antigens Antigens are macromolecules of natural or synthetic origin; chemically they consist of various polymers – proteins, polypeptides, polysaccharides or nucleoproteins. Antigens display two essential properties: first, they are able to evoke a specific immune response , either cellular or humoral type; and, second, they specifically interact with products of this immune response , i.e. antibodies or immunocompetent cells. A complete antigen – immunogen – consists of a macromolecule that bears antigenic determinants (epitopes) on its surface (Fig. 1). The antigenic determinant (epitope) is a certain group of atoms on the antigen surface that actually interacts with the binding site on the antibody or lymphocyte receptor for the antigen. Number of epitopes on the antigen surface determines its valency. Low-molecular-weight compound that cannot as such elicit production of antibodies, but is able to react specifically with the products of immune response, is called hapten (incomplete antigen) . antigen epitopes Fig. 1. Antigen and epitopes Antibodies Antibodies are produced by plasma cells that result from differentiation of B lymphocytes following stimulation with antigen. Antibodies are heterogeneous group of animal glycoproteins with electrophoretic mobility β - γ, and are also called immunoglobulins (Ig) . Every immunoglobulin molecule contains at least two light (L) and two heavy (H) chains connected with disulphidic bridges (Fig. 2). One antibody molecule contains only one type of light as well as heavy chain. There are two types of light chains - κ and λ - that determine type of immunoglobulin molecule; while heavy chains exist in 5 isotypes - γ, µ, α, δ, ε; and determine class of immunoglobulins - IgG, IgM, IgA, IgD and IgE .
    [Show full text]
  • EUROPEAN PHARMACOPOEIA Free Access to Supportive Pharmacopoeial Texts in the Field of Vaccines for Human Use During the Coronavirus Disease (COVID-19) Pandemic
    EUROPEAN PHARMACOPOEIA Free access to supportive pharmacopoeial texts in the field of vaccines for human use during the coronavirus disease (COVID-19) pandemic Updated package - October 2020 Published in accordance with the Convention on the Elaboration of a European Pharmacopoeia (European Treaty Series No. 50) European Directorate Direction européenne for the Quality de la qualité of Medicines du médicament & HealthCare & soins de santé Council of Europe Strasbourg Free access to supportive pharmacopoeial texts in the field of vaccines for human use during the coronavirus disease (COVID-19) pandemic Updated package The EDQM is committed to supporting users during the coronavirus disease (COVID-19) pandemic – as well as contributing to the wider global effort to combat the virus – by openly sharing knowledge and providing access to relevant guidance/standards. To support organisations involved in the development, manufacture or testing of COVID-19 vaccines worldwide, many of which are universities and small and medium-sized enterprises, the EDQM is offeringte mporary free access to texts of the European Pharmacopoeia (Ph. Eur.) in the field of vaccines. This package includes quality standards for vaccines which developers can take into account to help build appropriate analytical control strategies for their COVID-19 candidate vaccines and ensure the quality and safety of the final product. Application of such quality requirements may ultimately help to facilitate regulatory acceptance of a subsequent marketing authorisation application. For ease of reading, a summary table listing the pharmacopoeial texts, with information regarding the vaccine types or vaccine platforms concerned (e.g. live attenuated viral vaccine, recombinant viral-vectored vaccines) is provided.
    [Show full text]
  • Antibodies As Diagnostic Targets and As Reagents for Diagnostics
    antibodies Editorial Antibodies as Diagnostic Targets and as Reagents for Diagnostics Nicole H. Trier and Gunnar Houen * Department of Neurology, Rigshospitalet Glostrup, Valdemar Hansens vej 13, 2600 Glostrup, Denmark; [email protected] * Correspondence: [email protected]; Tel.: +45-3863-3863 Received: 19 April 2020; Accepted: 22 April 2020; Published: 18 May 2020 Antibodies (Abs) were discovered around the turn of the 19th century and characterized in the following decades as an essential part of the human adaptive immune system [1,2]. Abs are produced in response to infections with pathogenic organisms and therefore have diagnostic potential. The levels of specific Abs reflect the burden of infection and the type of Abs produced may reflect the duration of infection and the site of infection, since Abs undergo class switching and affinity maturation in the course of infections [1,2]. Initially IgM is produced, but switching to IgG, IgA or IgE occurs during infections, depending on the type of pathogen and the site of infection. The use of Abs as targets of diagnostics can roughly be divided in five areas: 1. Infections The specificity, type and concentration of Abs have diagnostic value. The specificity giving information on the type of pathogen, the type and concentration giving information on the state of infection (IgM as an indication of early infection, IgG and IgA as indications of prolonged or chronic infections (IgA further indicating mucosal/epithelial infection)) [2]. 2. Autoimmune Diseases The specificity, type and concentration of auto-Abs have diagnostic value. The specificity and the type giving information on the tissue/organ involved (IgA indicating mucosal/epithelial affection, IgM being relevant in a few conditions), the concentration giving some information on the degree of affection [3].
    [Show full text]
  • Modpathol201624.Pdf
    ANNUAL MEETING ABSTRACTS 509A 2022 Quick Review of Fine Needle Aspiration Accuracy with Focus on mcg/L, which correlates with cirrhosis, was 76% specific for C282Y. Most C282Y Cytohistologic Correlation and Evaluation of Discrepant Cases in a Limited heterozygotes (4) and C282Y/C282Y (13) patients with available results met these cut Sample offs, and two C282Y patients met criteria based on family history alone. Somaye Yekezare, Niloufar Reisian, Xiaoyan Liao, Farnaz Hasteh. University of Conclusions: The proposed criteria of TS>45%, ferritin>1,000 mcg/L, or a positive California San Diego Health System, San Diego, CA; Kaiser Medical Center, San family history, was 100% sensitive and moderately specific for C282Y screening. Diego, CA. Our institution is now considering an approach to include measuring ferritin/TS, and Background: Fine needle aspiration biopsy (FNAB) is a minimally invasive procedure reflexive HFE gene testing only when criteria are met. If used broadly, the algorithm commonly utilized for the primary investigation of mass lesions. Correlation of FNAB should detect all at-risk patients and result in an estimated 43% reduction of unnecessary diagnosis with histopathologic results is part of quality assurance and quality control HFE tests and a lower cost per new diagnosis. in cytology laboratories. The aim of this study was to investigate the diagnostic performance of FNAB and to identify the specimen types that are prone to errors. Design: A total of 1125 FNABs performed at our institutes in 2013 were selected and Techniques (including Ultrastructure) 401 satisfactory cases with the follow-up surgical specimens were included for this study. FNAB results were classified as negative, atypia, or positive; whereas final pathologic 2024 Complementary Value of Electron Microscopy and diagnoses were classified as malignant or benign.
    [Show full text]
  • Scope of Accreditation
    DIAGNOSTIC ACCREDITATION PROGRAM College of Physicians and Surgeons of BC Telephone: 604-733-7758 300–669 Howe Street Vancouver BC V6C 0B4 Toll free: 1-800-461-3008 (in BC) www.cpsbc.ca Fax: 604-733-3503 Laboratory Medicine – Scope of Accreditation Facility name Vancouver General Hospital Facility address 855 West 12th Avenue, Vancouver, BC, V5Z 1M9 Facility code 0610LM Corporate entity/health authority Vancouver Coastal Health Accreditation Full Accreditation Effective date 2019-03-28 Expiry date 2024-02-27 Laboratory Standards Version 1.4 Services The College of Physicians and Surgeons of BC Diagnostic Accreditation Program accredits the capability of the named facility to perform the listed services and examinations/procedures. Examination – Other Examination Methodology/principle – Other Sample Type – Other DAP Scope Classification (Analyte/Procedure) Methodology/principle Sample Type (Analyte/Procedure) (Specify) (Specify) (Specify) Sample Collection Sample Collection Venous and Capillary Whole Blood Sample Collection Sample Processing Receipt, accessioning, storage, Blood, fluids, stool, tissues, swabs, transportation foreign bodies Anatomical Pathology Autopsy Anatomical Pathology Fixation and Gross examination Fixation and gross examination Tissue - Formalin Fixed Anatomical Pathology Fixation and Gross examination Fixation and gross examination Tissue - Fresh Anatomical Pathology Immunofluorescence microscopy Microscopy - Fluorescent Tissue - Frozen Anatomical Pathology Immunohistochemistry - Class I p63, CD15, CX2, CD56 IHC
    [Show full text]
  • Distinction of Human Immunodeficiency Virus Type 1 Neutralization and Infection Enhancement by Human Monoclonal Antibodies to Glycoprotein 120 Akira Takeda,* James E
    Distinction of Human Immunodeficiency Virus Type 1 Neutralization and Infection Enhancement by Human Monoclonal Antibodies to Glycoprotein 120 Akira Takeda,* James E. Robinson, David D. Ho, Chrisfine Debouck, Nancy L. Haigwood, and Francis A. Ennis* *Division ofInfectious Diseases, Department ofMedicine, University ofMassachusetts Medical School, Worcester, Massachusetts 01655; Department ofPediatrics and Microbiology, Immunology and Parasitology, Louisiana State University School ofMedicine, New Orleans, Louisiana 70112; Division ofInfectious Diseases, The Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016; Department ofMolecular Genetics, Smith Kline Beecham Pharmaceuticals, King ofPrussia, Pennsylvania 19479; and Chiron Corporation, Emeryville, California 94608 Abstract reference to HIV vaccine development, since very low concen- trations of anti-HIV- 1 antibodies can enhance HIV-1 infec- There is increasing evidence that sera from HIV-1-infected tion. Robinson et al. first reported complement-mediated en- individuals contain antibodies that enhance infection by HIV-1 hancement of HIV- 1 infection in a T cell line bearing comple- in vitro. Previous work has demonstrated that complement re- ment receptors (1). We have previously demonstrated ceptors on T lymphoid cells and Fc receptors for IgG (Fc'yR) on antibody-dependent enhancement of HIV-l infection via Fc monocytic cells are required for enhanced infection by anti- receptor (FcR)'-mediated entry using a human monocytic cell body-complexed HIV-1. Characterization of such infection-en- line U937 that possesses FcRs for IgG (3). Heat-inactivated hancing antibodies is essential because immunogenic epitopes sera from HIV- 1 antibody-positive individuals enhanced infec- which induce enhancing antibodies should be excluded from tion ofU937 cells.
    [Show full text]