molecules Article Preparative Separation of Flavonoids from Goji Berries by Mixed-Mode Macroporous Adsorption Resins and Effect on Aβ-Expressing and Anti-Aging Genes 1,2, 3, 4 4 5 1,2, Jianfei Liu y, Jiao Meng y , Jinhao Du , Xiaofeng Liu , Qiaosheng Pu , Duolong Di * and Chang Chen 2,3,* 1 CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; jfl
[email protected] 2 University of Chinese Academy of Sciences, Beijing 100049, China 3 National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;
[email protected] 4 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;
[email protected] (J.D.);
[email protected] (X.L.) 5 State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou 730000, China;
[email protected] * Correspondence:
[email protected] (D.D.);
[email protected] (C.C.); Fax: +86-931-4968248 (D.D.); +86-010-64888406 (C.C.) These authors contributed equally to this work. y Received: 30 May 2020; Accepted: 21 July 2020; Published: 31 July 2020 Abstract: Flavonoids are the main constituents of Goji berries and have good biological and pharmacological activities. The mixed-mode macroporous adsorption resins (MARs) for purification of flavonoids from Goji berries through computer-assisted calculation of the molecular size of flavonoids and the precise matching of MAR physical and chemical properties was firstly developed in the present study.