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Stem Cell Research Useful Tools for the Study of the Control of Cell Fate MAR2011

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Stem Cell Research Useful Tools for the Study of the Control of Cell Fate MAR2011 Products for Stem Cell Research Useful tools for the study of the control of cell fate MAR2011 Item No. Product Name Summary 13123 BIO Inhibits GSK3α/β, which enables activation of the Wnt signaling pathway and sustained pluripotency in human and murine embryonic stem cells; maintains self-renewal in human and murine ESCs as well as induces the differentiation of neonatal cardiomyocytes 13124 BIX01294 Used in combination with the calcium channel activator BayK8644 to (hydrochloride facilitate the generation of induced pluripotent stem cells from somatic hydrate) cells in vitro 13187 Cardiogenol C Induces the differentiation of myosin heavy chain-positive cardiomyocytes (hydrochloride) from embryonic stem cells with an EC50 value of 0.1 µM; about 90% of embryonic stem cells treated with 0.25 µM of this compound express the cardiac muscle cell specific transcription factors GATA-4, MEF2, and Nkx2.5 and display the characteristic beating behavior of differentiated cardiomyocytes 13122 CHIR99021 An inhibitor of GSK3α and GSK3β that induces the reprogramming of murine and human somatic cells into stem cells 13352 EHNA A reversible adenosine deaminase inhibitor (IC50 = 1.2 μM in human red (hydrochloride) blood cells) that also selectively inhibits PDE2 (IC50s = 0.8 and 2 μM from human and porcine myocardium, respectively, 3.5 μM in rat hepatocyte, and 5.5 μM in human platelet); used to maintain pluripotency/prevent differentiation of human embryonic stem cells 10566 Garcinol An inhibitor of the HATs p300 and PCAF (IC50 = 7 and 5 μM, respectively); promotes neurogenesis and ex vivo expansion of human hematopoietic stem cells 13224 Neurodazine Induces neuronal differentiation in myoblasts and skeletal muscle cells; 2 μM for seven days upregulated the expression of neuronal markers such as neuron-specific enolase, neurofilament 200, and synapsin in murine C2C12 myoblasts 13196 NSC 23766 A cell-permeable, reversible inhibitor of Rac1 activation by the Rac-specific (hydrochloride) GEFs TrioN and Tiam 1 (IC50 = 50 μM); used to investigate the role of Rac1 in such diverse cellular functions as stem cell mobilization, epithelial cell migration, angiogenesis, leukemia cell migration and growth, and gene expression 13034 PD 0325901 A potent MEK inhibitor that suppresses phosphorylation of ERK; when combined with CHIR99021 (a GSK3 inhibitor) this compound can prevent cell differentiation and sustain self renewal of murine embryonic stem cells for at least eight passages 13032 PD 173074 A potent, selective inhibitor of FGFR tyrosine kinase activity that blocks autophosphorylation of FGFR1 (IC50 = 21.5 nM) and prevents signaling through FGFR2-5 at nanomolar levels; this compound impairs angiogenesis as well as self-renewal of stem cells via ERK1/2 activation 10009634 Purmorphamine Promotes the differentiation of both human and murine mesenchymal progenitor cells into osteoblasts (EC50 = 1 µM); directly binds to and activates the 7-transmembrane Smo receptor of the Hedgehog signaling pathway See reverse side for continued list for full information on these products and more visit caymanchem.com Products for Stem Cell Research continued Item No. Product Name Summary 10004412 Reversine A dedifferentiation agent of embryonic progenitor cells; cultured myoblasts treated for four days with 5 µM of this compound were dedifferentiated into confluent stem cell progenitors, that could then be induced by osteogenic or adipogenic medium to re-differentiate into bone or adipose precursors, respectively 10006606 Retreversine A control molecule for reversine that is inactive at 10-fold higher concentrations than reversine in the myoblast assay 10007955 CAY10498 A structural analog of reversine; however, dedifferentiation effects with this compound have not been demonstrated 13579 Salinomycin Shows selective toxicity for the cancer stem cells that exist as a subpopulation within HMLER breast cancer cells (sodium salt) (IC50s = ~24 versus ~90 μM); At 8 μM this compound reduces the number of cancer stem cells 2-3-fold in 4T1 and MCF7Ras breast cancer cell lines and induces increased epithelial differentiation of tumor cells 13031 SB 431542 A potent and selective inhibitor of the TGF-β1 receptor ALK5 (IC50 = 94 nM) that specifically blocks Smad signaling, suppressing renewal in embryonic and induced pluripotent stem cells and promoting their differentiation 10009557 SC-1 A small molecule activator of stem cell renewal that allows the propagation of OG2 mES cells for at least 10 passages in an undifferentiated state; this compound’s activity is mediated by the combined inhibition of RasGAP, which increases Ras signaling via the PI3-kinase pathway to promote self-renewal, and ERK1, which blocks differentiation (Kd = 98 and 212 nM, respectively) 10011251 TWS119 Potently inhibits GSK3β (IC50 = 30 nM) and induces neurogenesis in murine embryonic stem cells, making it a useful tool to regulate stem cell self-renewal and differentiation 13033 Valproic Acid Inhibits GSK3 and depletes cellular inositol-1,4,5-trisphosphate (1,4,5-IP3, demonstrating pronounced effects on stem cell (sodium salt) differentiation and self-renewal 10005583 Y-27632 A potent, ATP-competitive inhibitor of Rho-associated protein kinases including p160ROCK (Ki = 140 nM) and ROCK- (hydrochloride) II (IC50 = 800 nM) that blocks apoptosis of dissociated cultured human embryonic stem cells, increasing cloning efficiency by 25% and sustaining survival up to 30 passages Assay Kits Item No. Product Name Summary 10009853 CFSE Cell Cayman’s CFSE Cell Division Assay Kit provides an easy to use format for labeling and tracing cells through successive Division Assay Kit cell divisions which can be used to study the induction and inhibition of cell division in any in vitro model. The kit contains sufficient reagents for labeling and analyzing 100 cell samples by flow cytometry. CFSE can also be combined with any fluorochrome compatible with fluorescein for use in flow cytometry. 10009856 7-AAD Cell Cayman’s 7-AAD Cell Viability Assay Kit employs 7-AAD as a fluorescent label for dead cells. A fixative/actinomycin D Viability Assay Kit solution is included in the kit for cell fixation and blocking, making subsequent immunostaining of surface/intracellular antigens possible. This kit provides a convenient tool to quantify cytotoxic effects of environmental toxins or drug candidates. 600120 7-AAD/CFSE Cayman’s 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE to label target cells and 7-AAD to label Cell-Mediated dead cells. This kit provides a convenient tool to quantify the cytotoxic effect of immune effector cells at a single cell Cytotoxicity level. The kit provides sufficient reagents to effectively treat/stain 10 plates worth of cells irrespective of the number of Assay Kit wells per plate. Cayman Chemical • Ann Arbor, MI 48108 • 800.364.9897 • caymanchem.com APRIL2012.
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