Ophiuroidea: Euryalida: Gorgonocephalidae) from the East Sea, Korea
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Anim. Syst. Evol. Divers. Vol. 31, No. 4: 311-315, October 2015 http://dx.doi.org/10.5635/ASED.2015.31.4.311 Short communication A Newly Recorded Basket Star of Genus Gorgonocephalus (Ophiuroidea: Euryalida: Gorgonocephalidae) from the East Sea, Korea Donghwan Kim, Sook Shin* Department of Life Science, Sahmyook University, Seoul 01795, Korea ABSTRACT Euryalid specimens were collected from Gonghyeonjin and Daejin, Gangwon-do in the East Sea, Korea at a depth of 250-300 m by fishing nets on November 2013 and August 2014. They were identified as Gorgonocephalus arcticus Leach, 1819 belonging to family Gorgonocephalidae of order Euryalida, which was new to the Korean fauna. Nucleotide sequences of partial mitochondrial cytochrome c oxidase I (mt-COI) gene, which was 569 bp in length, were compared among four Gorgonocephalus species, and were subsequently employed to reconstruct phylogenetic trees using the MP, ML, and BI methods. As a result, no sequence difference was found between the G. arcticus mt-COI gene sequences from Korea and Canada, and the two made a strong monophyletic group. With the newly recorded G. arcticus in Korea, in total, four Gorgonocephalus species have been reported in Korea. Keywords: Gorgonocephalus arcticus, taxonomy, molecular phylogenetic analyses, mitochondrial COI gene INTRODUCTION gonocephalus species have been reported in Korea based on their morphology (Shin, 2013; Kim and Shin, 2015). Family Gorgonocephalidae, comprising 34 genera, is the Some basket stars were collected from Gonghyeonjin and largest of three families belonging to order Euryalida (Stöhr, Daejin, Gangwon-do in the East Sea, Korea at a depth of 2015), and its four genera (Astroboa, Astrocladus, Astroden 250-300 m by fishing nets on November 2013 and August drum, and Gorgonocephalus) have been reported in Korean 2014. The specimens were preserved in 95% ethyl alcohol fauna (Shin and Rho, 1996; Shin, 2013). Species of this and identified on the basis of morphological characteristics family have very branched five arms and have rings of little and molecular analyses. The important morphological char- hook-like spines forming bands around the branches of each acteristics were photographed using a digital camera (Nikon arm. Almost all Gorgonocephalus species are exclusively D7000, Tokyo, Japan), stereo-microscope (Nikon SMZ distributed in deep water and are of worldwide distribution 1000), and scanning electron microscope (JEOL JSM-6510, (Piepenburg, 2000). This genus has special features such as Tokyo, Japan). The sequences of mitochondrial cytochrome c the presence of arm spines before the first arm fork of arms, oxidase I (mt-COI) gene were analyzed with newly intended disk and arm covered with small stumps or tubercles, disk primers (F-TGRGCYGGVACMRYDGGAACHGC and R- often naked interradially (Baker, 1980), and the presence GGRTCHCCKCCHC CHGWDGGRTC) for the accurate of a row of marginal plates on the interbrachial outer mar- molecular identification of KoreanGorgonocephalus species. gin (Matsumoto, 1917). A family-level revision of order DNA was extracted using DNeasy Tissue and Blood Kits Euryalida based on sequences from mitochondrial genes (Qiagen, Hilden, Germany), and PCR and phylogenetic ana- (16S rRNA and cytochrome c oxidase I [COI]) and a nuclear lyses were conducted according to Lee and Shin (2011) with gene (18S rRNA) from 83 euryalid ophiuroids including G. minor revision. The phylogenetic trees were inferred from chilensis, G. eucnemis, G. pustulatum, and G. tuberosus their alignment sequences by maximum parsimony (MP), was investigated (Okanishi and Fujita, 2013). Only three (G. maximum likelihood (ML), and Bayesian inference (BI) dolichodactylus, G. eucnemis, and G. tuberosus) of 10 Gor methods. They were identified as Gorgonocephalus arcticus This is an Open Access article distributed under the terms of the Creative *To whom correspondence should be addressed Commons Attribution Non-Commercial License (http://creativecommons.org/ Tel: 82-2-3399-1717, Fax: 82-2-3399-1729 licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, E-mail: [email protected] and reproduction in any medium, provided the original work is properly cited. pISSN 2234-6953 eISSN 2234-8190 Copyright The Korean Society of Systematic Zoology Donghwan Kim, Sook Shin posed ofsharpspiniformteeth tic Ocean tic chial outermargin platesoninterbra small granules,witharowofmarginal Aug 2014,at300mdepthbyfishingnet. Gangwon-do, 29 Goseong-gun, Port, Hyeonnae-myeon, Daejin 1 specimen, Seoul, Korea. various sizes and dorsal sideofarms denselycovered with granules of SYSTEMATIC ACCOUNTS Re Echinoderm Marine inthe deposited are These specimens Leach, 1819, which is reported for the first time in Korea. 312 Korean arm segment arm cylindrical, withmanysmallpores,halfofcorresponding Arm spine short, arm segments onventralsideofarm. Gangwon-do, 12Nov2013,at250 Goseong-gun, Jugwang-myeon, Gonghyeonjin-ri, Port, Gorgonocephalus arcticus Gorgonocephalus Gorgonocephalus agassizi Astrophyton side ofdisk side ventral of corner on a trace left madreporite One of disk. towards center features tapering concave lengths and widely of Mexico. Gulf America, Norway, Kara Sea,FranzJosefLand),North the northwest North Atlantic Ocean the northwestNorth cold boreal zonearound in the species usually distributed pointed tips near endofarm near tips pointed alcohol. Description. Material examined. Material and Cochrane,2003:126. Anisimova 1990:446;1994:161; Smirnov andSmirnov, 1954:27; 1924: 231;Mortensen,1933:9;D’yakonov, 1 Genus Ljungman,1867 Family Gorgonocephalidae Order EuryalidaLamarck,1816 1840 Class OphiuroideaGray, Remarks. Size. Distribution. Color. source Bank of Korea of Bank source * Gorgonocephalus arcticus name: R Color is yellow ocher in lifeandislightbrown in Colorisyellowocher Gorgonocephalus = (Greenland, Spitzbergen, Barents Sea, White Sea, White Barents Sea, Spitzbergen, (Greenland, (Fig. 1I). Three or four arm spines exist between between exist spines orfour arm Three 1I). (Fig. 2.5 1 This northern basket star is an endemic arctic endemic arctic star isan This northernbasket * (Fig. 1F, G), becoming hooks with two or three orthree two hooks with G),becoming (Fig. 1F, 북극삼천발이 (Fig. 1C, H). Radial shields have different (Fig. 1C,H).Radialshieldshavedifferent cm, r Disk with thin plates, covered with many with many Diskwiththin plates,covered Korea agassizii (Fig. 1A, B). Especially, radial shields radialshields (Fig. 1A,B).Especially, : Lyman, 1865:186. : Lyman, = 1.5 (East Sea), Siberian East Coast, Arc Coast,Siberian East (East Sea), Korea: 1 specimen, Gonghyeonjin Gonghyeonjin 1 specimen, Korea: ( 신칭 (MERBK), Sahmyook University, University, Sahmyook (MERBK), cm, R/r : Döderlein, 1911: 103; Koehler, 103;Koehler, : Döderlein,1911: Leach,1815 : Lyman, 1882:264. : Lyman, ) (Fig. 1J (Fig. 1D,E). Leach,1819(Fig.1) = (Piepenburg, 2000), and (Piepenburg, m depth by fishing net; 1.6. - M). Oral parts com M). Oral - - - Table 1. Polymorphic sites between Korean G. arcticus and four Gorgonocephalus species obtained from NCBI 0 0 0 0 0 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 4 4 4 4 5 5 5 5 5 5 5 Scientific name 0 2 3 5 9 0 2 3 3 5 5 5 6 9 2 2 4 4 5 7 8 8 9 0 0 3 5 9 9 0 3 6 8 0 0 1 2 3 4 6 3 4 4 1 9 8 3 2 8 1 6 9 2 2 2 5 3 6 5 9 2 9 4 6 9 0 7 3 6 3 9 2 0 4 7 0 8 7 3 1 G. tuberosus G A A C C C C T A T C C G A A C C T C A G T T C T T T A T T T A C T T C T C C T (AB758811) G. chilensis . C . T . C . C . Anim. Syst.Evol.Divers. 31(4),311-315 (AB758812) G. eucnemis A . C . T . T . C . T . C . A T A T . A C C T . C . C A T A (GU670194) G. arcticus (HM543019, A G C A T T . A G C T T A C T . T . T C A . C T A C A C A A C C T A A T C A T A Canada) G. arcticus (KR919684, A G C A T T . A G C T T A C T . T . T C A . C T A C A C A A C C T A A T C A T A Korea) The total aligned sequence number was 569 bp. There were no sequence differences between KR919684 (Korea) and HM543019 (Canada). A Newly Recorded Gorgonocephalus Basket Star from East Sea, Korea A B C D E F G H I J K L M Fig. 1. Gorgonocephalus arcticus. A, C, Dorsal view; B, D, Ventral view; E, Oral part; F, Ventral side of arm; G, Lateral side of arm; H, Distal part of radial shield; I, Madreporite; J, K, Arm spines under scanning electron microscope; L, M, Arm spines under light microscope. Scale bars: A, B=2.5 cm, C, D=1.4 cm, E-I=3 mm, J-M=50 μm. Anim. Syst. Evol. Divers. 31(4), 311-315 313 Donghwan Kim, Sook Shin Fig. 2. Maximum parsimony tree inferred from mt-COI, showing phylogenetic relationships among four Gorgonocephalus species. Bootstrapping values obtained from maximum parsimony, maximum likelihood, and Bayesian inference methods are shown in each node in order. G. Gorgonocephalus; O. Ophiopholis. is characterized having a smooth and thick body, distinctly isms causing Ecolo gical Disturbance and Harmful Effects, up welled disk, and acute spiniform teeth. funded by KIMST/MOF, Korea. Molecular analysis. A total of 569 base pairs (bp) of mt- COI DNA were obtained from Korean G. arcticus (GenBank accession number: KR919684), and perfectly correspond to REFERENCES G. arcticus sequence data obtained from NCBI (GenBank HM543019, Canada). There were partial sequence gaps Anisimova NA, Cochrane SJ, 2003. An annotated checklist comparing to each sequence of other Gorgonocephalus of the echinoderms of the Svalbard and Franz Josef Land species obtained from NCBI: difference as 36 bp from G.