www.epic-assoc.com/epic-biophotonics-symposium-and-exhibition

Talks by medical doctors: 15 minutes + 5 minutes Q&A Talks by companies, universities and research organizations: 10 minutes + 5 minutes Q&A

Please note that timing is strictly respected at EPIC meetings. No company pitch, focus on technology. This is an EPIC technology workshop fostering exchange among actors of the value chain. Prior to the event, a list of participants including biography + picture of each attend and company description will be shared. The event is limited to 75 attendees to ensure efficient networking.

25 NOVEMBER 2015

EPIC members, EPIC committee, and medical doctors only (free of charge, registration required) 20:00 Dinner – Location to be announced.

26 NOVEMBER 2015

EPIC members, EPIC committee, and medical doctors only (free of charge, registration required) 08:30 Welcome Coffee 09:00 Introduction to Elisabeth Klinik - Zentrum Lasermedizin by Prof. Dr.med. H.-Peter Berlien 09:15 Medical doctor presentation + discussion 10:00 Coffee Break 10:30 Medical doctor presentation + discussion 11:15 Departure to Messe Berlin for EPIC Workshop (bus / car / public transport)

12:00 REGISTRATION / SNACKS / NETWORKING MARKETPLACE

Session 1: Photonics-enabled in-vivo Imaging and Spectroscopy in Oncology

Cellular microscopy techniques (confocal, spectroscopy, Raman, etc.) are already available to provide accurate characterization of tissues but the true challenge now is to bring these microscopic optical approaches into endoscopic instruments. This in vivo microscopy will contribute significantly to improve the diagnosis of several pathologies in the clinic.

Topic 1 : Label-free Imaging Modalities for in-situ resection monitoring

13:00 EPIC Welcome, Carlos Lee, Director General

13:10 “Application of spectroscopic methods for the prevention of side effects on patients during chemotherapy” by Prof. Dr. Dr.-Ing Juergen Lademann, M. Darvin, H. Richter, A. Patzelt, M. Meinke, S. Jung. Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité – Universitätsmedizin Berlin, Berlin, Germany

Although chemotherapeutics for cancer treatment are becoming increasingly efficient these days, they often cause severe dermal side effects. Systemically applied doxorubicin is known for inducing free radicals, which lead to the development of the hand-foot syndrome. This syndrome manifests itself through skin irritations via blistering to open wounds. As doxorubicin exhibits a fluorescence signal in the 520-600 nm region if excited at 488 nm, the doxorubicin’s leakage onto the skin surface could be analysed. It was found that part of the doxorubicin is ejected with the sweat onto the skin surface, where it spreads and penetrates into the skin like topically applied. This process is prominent especially in the palmar and plantar region where the density of sweat glands is highest. By topical application of antioxidants the doxorubicin could be prevented from inducing free radicals in the skin and consequently the hand-foot syndrome.

13:30 “Real-time multispectral imaging for biomedical applications” by Dr. Nikolaos Deliolanis, Group Leader, Fraunhofer IPA-PAMB Multispectral (MS) imaging of tissue can reveal anatomical, functional and pathological information by identifying concentrations of absorbers like oxy- and deoxy-hemoglobin and, additionally, multiple intrinsic fluorochromes or externally administered probes. State of the art MS imaging systems are limited: they can image only one fluorochrome, and not simultaneously with color images. We present an imaging platform based on a combined approach of temporal and spectral multiplexing that acquires MS fluorescence and reflectance images over the entire VIS/NIR spectrum without spectral gaps and unmixes the images to 6 fluorescence and 6 reflectance components. It can be directly adapted to existing systems like surgical microscopes or rigid endoscopes. We present results from preclinical experiments of fluorescence guided in-vivo tumor resection.

13:45 “OCT: an excellent match between what we have and what we want for the next generation medicine, applied to cancer” by Frans Dhaenens, Clinical Research Consultant, Agfa HealthCare The current trend of "precision medicine" points towards diagnostic and therapeutic genetic- determined biological processes with a personal basis. The medical model shifts towards sensitive and specific "point-of-care" detection technologies often multiplexed, generating huge datasets, which can be analysed in a meaningful way because they obey well established laws of physics giving sight on "quantum-biologics". Photonic imaging methods have the potential to span the classic divide between macro-imaging and histopathology. When offered at an attractive cost, photonics are accepted easily in a rather conservative daily workflow. The approach of photonic imaging to the growing cancer problem is demonstrative for its potential in medicine.

14:00 “Optical in vivo detection of cancer by Raman microspectroscopy” by Dr. Oliver Duehr, Senior Sales Engineer, Ocean Optics At present the success of a standard cancer treatment is investigated by biopsy and laboratory analysis. With the demonstrated in vivo Raman microspectroscopy it is possible to accelerate this process down to a few seconds to get information about the success of the cancer treatment and to distinguish between either vital or necrotic tissue by Raman spectra and assignment of the Raman spectral clusters. Although a classification model for the spectral data is used, the discrimination of necrotic and vital tissue yield an accuracy of 100%. The results makes Raman spectroscopy a powerful candidate for in vivo carcinogenic tissue analysis.

14:15 “The future of bio-optics in peri-operative surgical oncology” by Tim Clay, Associate Director, Global Medtech, Cambridge Consultants Significant advances have been achieved in real-time optical imaging strategies for intraoperative tumour detection and margin assessment. Optical imaging holds promise in achieving the highest percentage of negative surgical margins and in early detection of micrometastastic disease over the next decade. Currently the use of imaging modalities for guidance during surgical interventions has been limited to incidental use of intraoperative X-ray fluoroscopy and ultrasonography. However, there is a rapidly developing landscape for novel optical technologies, which will uncover the detailed micro-structure of tumour pathologies in situ and provide surgeons with new tools and techniques for successful patient treatment. We intend to provide a review of such techniques and weigh up the significance of such technologies.

14:30 COFFEE BREAK & NETWORKING IN MARKETPLACE

Topic 2 : Progress in Fiber Optics for Minimally Invasive Surgery & Diagnostic

15:00 “In vivo study for the discrimination of cancerous and normal skin using fibre probe-based Raman spectroscopy” by Asst. Prof. Martina C. Meinke1, J. Schleusener 2, P. Gluszczynska 2, C.Reble1,2, I. Gersonde 2, J. Helfmann 2, JW Fluhr1, J. Lademann , H. Röwert 1, A. Patzelt1 1 Department of Dermatology, Venerology and Allergology, Charité – Universitätsmedizin Berlin, Germany 2 Laser- und Medizin-Technolgie GmbH Berlin, Berlin, Germany

The application of fiber-coupled Raman probes for the discrimination of cancerous and normal skin has the advantage of a noninvasive in vivo application, easy clinical handling, and access to the majority of body sites, which would otherwise be limited by stationary Raman microscopes. In this in vivo clinical evaluation, the probe design is optimized for epithelial sensitivity, whereby a large part of the detected signal originates from within the epidermal layer’s depth down to the basal membrane where early stages of skin cancer develop.

15:30 Bioresorbable optical fibres: status and perspectives” by Daniel Milanese, Associate Professor, Politecnico di Torino Typically, the implementation of optical fibres to the biomedical field have foremost focused on the bio-compatibility of the fibre material. The development of bioresorbable optical fibres could pave the way to novel biomedical devices that combine several health diagnostic functionalities in compact format. The main types of currently available bioresorbable optical fibres will be reviewed together with their advantage and their range of applications.

15:45 "Specialty Optical Fibers Make Surgery Less Invasive" by Chris Emslie, CEO, Fibercore The use of specialised coatings, appropriately selected and processed enable fibers to survive multiple cycles of autoclave sterilisation. Careful matching and tight control of optical parameters facilitate optical coherence tomography (OCT) used in non-contact surface profiling for opthalmology as well as the precise navigation of blood-vessels to enable the safe and even de-skilled insertion of guide-wires. The incorporation of minute, intrinsic sensors into probes make the measurement of blood-flow and even gas analysis possible. The next generation of catheters and guide-wires, combining multi-core and FBG technologies may even render X-ray obsolete in many procedures by providing real-time information of the exact shape of the probe.

16:00 "Fiber spectroscopy for biophotonics - methods & sensors for promising applications" by Dr. Viacheslav Artyushenko, President, art photonics Spectral fiber sensors development described to detect tumor margins – due to the need to reduce 30% of incomplete tumor removal in surgery. Comparison of the best spectroscopy method (or their synergy combination): Raman scattering, fluorescence, Mid IR-absorption and diffuse Vis/NIR- reflection was made for the normal and malignant parts of the same organ (in-vitro samples of kidneys from 6 patients) – to select which way may enable tissue differentiation by combination of the key clinical parameters: sensitivity, specificity and accuracy.

16:15 COFFEE BREAK & NETWORKING IN MARKETPLACE

Session 2: Match light engines features with biophotonics requirement

Strong variety of tissues requires new wavelength to meet right trade-off between absorption and scattering as well as more power to go deeper in biological tissues. Time resolved imaging needs to match biological time constants from second to picoseconds. Beside intrinsic performance that enables the entry of photonics components in biophotonics application, cost and integration will be key for future massive deployment.

Topic 1 : Laser moving towards higher power, tunability and new wavelength

16:30 “Mid-IR swept lasers for biophotonics applications” by Stephen J Matcher, Reader in Biomedical Engineering, Sheffield University We will describe work we have undertaken in order to translate advances in near-IR swept lasers into the mid-IR. Near-IR swept lasers have been heavily developed to drive optical coherence tomography (OCT) applications. They are often preferred over broad-band superluminescent and supercontinuum sources because a) they offer a fundamental and very large (x100 to x1000) sensitivity advantage over time-domain OCT when using single-element detectors and b) they avoid the need for array detectors in fourier-domain OCT. By exploiting an analogy between time-domain OCT and Fourier Transform Infrared (FTIR) spectroscopy we show that mid-IR swept lasers potentially can bring similar advantages to vibrational spectroscopy. Further development of mid-IR swept lasers can potentially drive new applications in diverse areas of medicine, materials science and medicine.

16:45 “The CO laser – filling an important wavelength gap for medical applications” by Matthias Schulze, Director of Marketing, Coherent This presentation will introduce a disruptive mid-infrared laser technology – the CO laser released earlier this year at Laser Munich. Based on electronic and gas plasma technologies long-proven in commercial CO2 lasers, the CO laser delivers high power CW or pulsed output at a wavelength of 5 microns. This is in the middle of the mid-infrared “wavelength gap” between solid state and fiber lasers in the 1-2 micron range and CO2 lasers at 9-10 micron. Being stronger absorbed by water than CO2 lasers the CO laser offers potential for improved aesthetic and surgical procedures. CO lasers also have disruptive advantages with tighter focusability, better depth of field and a path towards fiber delivery.

17:00 "Novel concepts on multi-color femtosecond fiber lasers and cw diode lasers for various type of microscopy" by Thomas Renner, EVP Sales & Marketing, TOPTICA Advanced optical microscopy methods nowadays rely strongly on dedicated laser based light engines. For linear microscopy flexibility in choice of wavelength, pulsing-on-demand and multiple fiber delivery ports is required. For non-linear microscopy alternatives to bulky and expensive femtosecond Ti:Sa lasers are desired for quite some time. This presentation will show two latest technology advances, which are dedicated to various microscopy applications: Next generation cw diode based multi-laser engines with an option to field-upgrade up to 8 wavelengths as well as novel concepts for multi-color femtosecond all-fiber lasers with time synchronized multi-modal laser pulses.

17:15 “Ultrafast lasers for life science applications” by Lukas Krainer, CEO, Onefive Ultrafast lasers experience a wide spread of use nowadays in various life science (and related) applications, from medical, imaging, micromanipulation to free electron laser facilities. Current use and future challenges will be presented.

17:30 “Configurable multi-indication medical lasers for oncology” by Dr. Petteri Uusimaa, CEO, Modulight Clinical use of medical lasers has become a routine in many fields, yet true multipurpose medical systems are hardly available. We present a solution for a multi-indication medical laser system for oncology that can be configured for different indications and treatment modalities. The ML7710 medical laser supports multiple drugs and treatment configurations in photoimmunotherapy and also direct hyperthermal tumor killing from a single CE marked medical laser. The system supports automated light dose calibration and monitoring via an easy to use graphical user interface.

17:45 PANEL DISCUSSION moderate by Gerwin Puppels, CTO & Managing Director, RiverD

18:15 End

18:15 Shuttle to Dinner – Departure Foyer Grosser Stern

18:45 Dinner at Hamburger Bahnhof, historical location directly on the Spree river

27 NOVEMBER 2015

08:30 Arrival Participants

Topic 2 : LED entry in Biophotonics application

09:00 “Improving fluorescence assisted cystoscopy of bladder tumours using a high power LED light engine” by Dr. Lars R. Lindvold, Center for Nuclear Technologies, Technical University of Denmark and Dr. Gregers G. Hermann, Urological Research Unit, Bisperbjerg/Frederiksberg Hospital, Denmark This presentation pertains to the development a new high power green LED optical engine that can substitute the conventional high-pressure xenon discharge lamps used in fluorescence assisted cystoscopy of bladder tumours. We will present in vivo results showing how this optical engine can excite Hexvix® induced fluorescence of bladder tumours (photodynamic diagnostics) and autofluorescence of the bladder mucosa thereby enabling full view of the bladder purely based on fluorescent imaging.

09:15 “Special light sources for Optogenetics and bioanalytics” by Dr. Torsten Ledig, Sales Manager, AMS Technologies The presentation will address the advantages of LEDs in optogenetics. Optogenetics as technology allows targeted, fast control of precisely defined events in biological systems. It provides the necessary speed and the precision required for biological processing. LEDs are able to simultaneously meet both the speed and the spatial precision requirements in millisecond scale and cell type specific, LEDs excel lasers in almost every respect: they are cheaper, smaller, more reliable, and easier to control.

Topic 3 : Integrated schemes for light transport and diffusion

09:30 “Light stimulated healing of chronic wounds by use of optical waveguides and light management microstructures” by Dr. David Kallweit, Senior Project Manager, CSEM Chronic wounds are creating costs of approximately 40 billion € annually. Recent studies have proven the therapeutic effects of visible light on the self-healing process. In this presentation the first results of the development of a device for medical wound care using large area disposable and flexible waveguides with incorporated micro- and nanometer scale light management structures is presented.

09:45 “Control the light where you need it; new development in accurate delivery of visible laser light” by Joost van Kerkhof, CEO, XiO Photonics Most biophotonic applications require high quality laser light which is accurately delivered to one or more defined locations. The possibility to realize this with conventional free space optics becomes a limiting factor due to increased complexity and decreasing form factors. The photonic integrated circuit technology of XiO Photonics enables applications where flexible and accurate delivery is critical. In this presentation we will show some specific application examples and the possibilities of this new technology.

10:00 “Integrated photonics based on nanophotonic structures for medical applications” by Dr. Anna Lena Giesecke, Group Leader Nanophotonics, AMO Functional nanostructures are beneficial for optical imaging in high sensitivity applications. Compactness and highly functionality using multiple wavelengths enable enhanced methods for diagnosis. The opportunity of high resolution measurements will be also discussed. The range of applications is wide such as OCT and spectroscopy.

10:15 “Linear Variable Filters for Fluorescence and Hyperspectral Imaging Applications” by Oliver Pust, Director Sales and Marketing, Delta Optical Thin Film Linear Variable Filters recently reached quality levels that make them applicable for demanding biomedical and hyperspectral imaging applications that often rely on fluorescence techniques. LVF offer tunability over a wide wavelength range with excellent blocking.

10:30 COFFEE BREAK & NETWORKING IN MARKETPLACE

Session 3: Towards Higher-Content with Photonics

11:00 “Actinic keratosis, squamous cell carcinoma and healthy skin – morphologic differences and similarities obtained in vivo using multiphoton tomography” by Dr. rer. medic. Maxim E. Darvin1, M. Klemp1, M.C. Meinke1, M. Weinigel2, K. König2,3, J. Lademann1 1Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité – Universitätsmedizin Berlin, Germany 2 JenLab GmbH, Schillerstr. 1, 07745 Jena, Germany 3 Dept. of Biophotonics and Laser Technology, Saarland University, Campus A5.1 66123 Saarbrücken, Germany

Patients with clinical diagnosis of actinic keratosis (AK) and squamous-cell carcinoma (SCC) were examined noninvasively using two-photon tomography at subcellular resolution. Changes in the morphology of the keratinocytes, such as broadened epidermis, large intercellular spaces, enlarged nucleus and a large variance in cell shape were investigated for the stratum granulosum (SG), stratum spinosum (SSp) and stratum basale (SB). The cell density in AK and SCC was significantly lower than in the SB and SSp cell layers of healthy skin. In SCC, the cell density was significantly lower than in AK. The size of the AK and SCC nuclei was significantly larger than that of healthy skin cells in the SSp and SB but not for the SG cell layer. The nucleus-to-cytoplasm ratio was significantly higher for AK and SCC for the SG and SSp but not for the SB cell layer.

11:30 “InSPECT – A broadband, integrated spectrometer device for spectral tissue sensing” by Dr. J.J.H.B. (Jean) Schleipen, Principal Scientist, Optics Group, Philips Minimally-invasive image-guided procedures – such as taking biopsies in screening suspicious nodules for cancerous tumors – are becoming increasingly important in clinical practice. Real-time tissue-characterization feedback during these procedures can significantly reduce false negatives, significantly improve the outcome of diagnosis and treatment, and reduce the cost of oncology treatment. Spectral tissue sensing using photonic needles has the promise to be a valuable tool for screening tumors, as shown by several clinical studies. The InSPECT project will develop photonic building blocks for low-cost miniaturized spectral tissue sensing devices. This involves the realization of a miniature broad-band (400-1700 nm) solid-state light source, based on phosphor and quantum- dot converted LEDs, and the realization of a miniature low-cost integrated VIS/NIR/SWIR spectrometer.

11:45 “FT-IR system for fast in vitro screening test” by Hughes Tariel, CEO, Diafir FT-IR spectroscopy has been described as a promising tool for simple and quick In vitro diagnostic for years. However, it failed to enter the clinical rooms, mostly due to sampling technique that required spectroscopy specialist to operate them. Fiber Evanescent Wave spectroscopy, that allows measurement by simple contact with the sample, can be a solution. We got promising result for infection and chronic disease detection. Oncology is the next step.

12:00 Closing Remarks

12:15 Lunch & Networking in Marketplace

Session 4: Future Trends in Biophotonics

14:45 FAREWELL DRINK AND SNACK

15:30 END

EPIC Pub 15-09-25 Magazine.qxp 25/09/15 15:53 Pagina 1

EPIC Biophotonics Symposium and Exhibition 26-27 November 2015 | Berlin, Germany

EPIC events are very well organised, and every time I get to know interesting people. Often mutual business opportunities emerge from these new contacts. bbee ppaarrtt ooff iitt!! Oliver Pust, DirectOr sales anD Marketing, Delta OPtical thin FilM a/s In partnership with micro photonics and the support of Berlin Partner

EPIC Biophotonics Symposium and Exhibition •» Session 1: Photonics-enabled Imaging and Therapies in Oncology • Label-free imaging modalities for in-situ resection monitoring • Progress in fiber optics for minimally invasive surgery

•» Session 2: Matching Light Engines Features with Biophotonics Requirement • Light engines, towards higher power and tunability • Integrated schemes for light transport and diffusion

•» Session 3: Towards Higher-content with Photonics In-Vitro Modalities • Cost effective spectroscopy for ex-vivo samples • New concepts in microscopy www.epic-assoc.com/epic-events

EPIC is the industry association that promotes the sustainable development of organisations working in the field of photonics in Europe. We foster a vibrant photonics ecosystem by maintaining a strong network and acting as a catalyst and facilitator for technological and commercial advancement. EPIC publishes market and technology reports, organizes technical workshops and B2B roundtables, coordinates EU funding proposals, advocacy and lobbying, education and training activities, standards and roadmaps, pavilions at exhibitions. www.epic-assoc.com/membership Coherent, Inc. Superior Laser Reliability and Performance.

Coherent was founded 1966 and is one of the leading manufacturers of laser technology. Headquartered in Santa Clara, CA USA it employs more than 2,400 employees at 14 manu- facturing sites in North America, Europe and Asia.

Coherent differentiates itself from other laser manufacturers by offering the broadest product portfolio available. It includes laser technology from ultraviolet to far infrared wavelength, from femtosecond pulse lengths to continuous wave radiation and from a few milliwatts to multiple kilowatts in power.

Coherent is active in the following markets and applications: • Microelectronics: Flat Panel Display, PC Board, Semiconductor and Photovoltaic Manufacturing • Materials Processing: Cutting, Welding, Marking and Engraving, Cladding, Heat Treating and Rapid Prototyping • Scientific Research: Multiphoton Microscopy and Spectroscopy • OEM & Instrumentation: Cytometry, Microscopy, DNA Sequencing, Refractive Surgery

Coherent’s key technologies are: • CO2 Lasers • Diode Lasers • Excimer Lasers • Diode-Pumped Solid-State Lasers • Fiber Lasers • Optically Pumped Semiconductor Lasers • High Power Diode Lasers • Ultra Short Pulse Lasers • Laser Machining Tools • Laser Diagnostics and Measurement • UV Optical Systems

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