Bull. Eur. Ass. Fish Pathol., 34(1) 2014, 25

Borderline ovarian mucinous cystadenoma with ’—ŸŠœ’˜—ȱ˜ȱœ›˜–Šȱ’—ȱ‘Žȱ˜•ęœ‘ȱ Carassius auratus (L.)

L. A. Romano1Șǰȱǯȱ •˜œŽ›‘˜ě2ǰȱǯȱû‘›2, R. V. Rodrigues3, M. B. Tesser3, M. A. R Garrido-Pereira1 and L. A. Sampaio3

1ȱŠ‹˜›Šà›’˜ȱŽȱ –ž—˜•˜’ŠȱŽȱŠ˜•˜’ŠȱŽȱ›Š—’œ–˜œȱŠ›’—‘˜œǰȱ —œ’ž˜ȱŽȱŒŽŠ—˜›ŠęŠǰȱ—’ŸŽ›œ’ŠŽȱ ŽŽ›Š•ȱ˜ȱ’˜ȱ ›Š—ŽǯȱŠ’¡Šȱ˜œŠ•ȱŚŝŚǰȱȱşŜǯŘŖŗȬşŖŖǰȱ’˜ȱ ›Š—Žǰȱǰȱ›Šœ’•Dzȱ2 Programa de Pós- ›ŠžŠ³¨˜ȱŽ–ȱšž’Œž•ž›Šǰȱ—’ŸŽ›œ’ŠŽȱŽŽ›Š•ȱ˜ȱ’˜ȱ ›Š—ŽǯȱŠ’¡Šȱ˜œŠ•ȱŚŝŚǰȱȱşŜŘŖŗȬşŖŖǰȱ ’˜ȱ ›Š—Žǰȱǰȱ›Šœ’•Dzȱ3ȱŠ‹˜›Šà›’˜ȱŽȱ’œŒ’Œž•ž›ŠȱœžŠ›’—ŠȱŽȱŠ›’—‘Šǰȱ —œ’ž˜ȱŽȱŒŽŠ—˜›ŠęŠǰȱ —’ŸŽ›œ’ŠŽȱŽŽ›Š•ȱ˜ȱ’˜ȱ ›Š—ŽǯȱŠ’¡Šȱ˜œŠ•ȱŚŝŚǰȱȱşŜŘŖŗȬşŖŖǰȱ’˜ȱ ›Š—Žǰȱǰȱ›Šœ’•

Abstract Žȱ›Ž™˜›ȱŠ—ȱ˜ŸŠ›’Š—ȱŒ¢œŠŽ—˜–Šȱ’—ȱŠ—ȱŠž•ȱ˜•ęœ‘ǯȱ‘ŽȱœŠ—Š›ȱ‘’œ˜™Š‘˜•˜’ŒȱŽ¡Š–’—Š’˜—ȱ ˜ȱ‘Žȱž–˜ž›ȱ–ŠœœȱŒ˜—Š’—ŽȱŠ›ŽŠœȱ˜ȱ–Šž›ŽȱŒ¢œ’Œȱ’œœžŽȱ‘ŠȱŽ¡‘’‹’Žȱ¢™’ŒŠ•ȱŠ••ȱŒ˜•ž–—Š›ȱŒŽ••œȱ with basally situated nuclei and abundant intracellular mucins stained with Alcian blue. Sections ˜ȱ‘Žȱ’œœžŽȱœ‘˜ ŽȱŠ—ȱ’—ŸŠœ’ŸŽȱœ›˜–Š•ȱ’–™•Š—ȱ˜ȱ‹˜›Ž›•’—Žȱ–žŒ’—˜žœȱŒ¢œŠŽ—˜–ŠȱŠœœ˜Œ’- ŠŽȱ ’‘ȱŒ•žœŽ›œȱ˜ȱŠŽ—˜ŒŠ›Œ’—˜–ŠȬ•’”ŽȱŒŽ••œǯȱ‘Žȱ’––ž—˜‘’œ˜Œ‘Ž–’ŒŠ•ȱœž’Žœȱœ‘˜ Žȱ‘Žȱ ¢™’ŒŠ•ȱ’––ž—˜‘’œ˜Œ‘Ž–’ŒŠ•ȱ™ŠĴŽ›—ȱ˜ȱœŠ’—’—ȱ˜ȱ ŝȱ˜ž—ȱ’—ȱŒ•Šœœ’ŒŠ•ȱ–žŒ’—˜žœȱŒ¢œŠŽ—˜–Šǯȱ ‘Žȱ’—ŸŠœ’ŸŽȱœ›˜–Š•ȱ’–™•Š—ȱŠ•œ˜ȱœ‘˜ Žȱ™˜œ’’ŸŽȱœŠ’—’—ȱ ’‘ȱ‘Žȱ ŝȱŠ—’‹˜¢ǯȱ‘Žȱž’•’¢ȱ˜ȱ Œ¢˜”Ž›Š’—ȱŝȱǻ ŝǼȱŽ¡™›Žœœ’˜—ȱ’œȱ›Žœ›’ŒŽȱŠ—ȱŠ••˜ œȱ˜ŸŠ›’Š—ȱ–žŒ’—˜žœȱŠŽ—˜–Šœȱ˜ȱ‹Žȱ’쎛Ž—’- ŠŽȱ›˜–ȱ•˜ Ž›ȱŠœ›˜’—Žœ’—Š•ȱ—Ž˜™•Šœ–œǰȱŠœȱ‘ŽȱŽ¡Ž—ȱ˜ȱœŠ’—’—ȱ˜›ȱ ŝȱ‘Šœȱ‹ŽŽ—ȱœ‘˜ —ȱ˜ȱ‹Žȱ ’Š—˜œ’ŒŠ••¢ȱ›Ž•ŽŸŠ—ȱ˜›ȱ‘Žȱ’œ’—Œ’˜—ȱ˜ȱ™›’–Š›¢ȱŠ—ȱœŽŒ˜—Š›¢ȱ–žŒ’—˜žœȱž–˜›œȱ˜ȱ‘Žȱ˜ŸŠ›¢ǯ

Introduction Ž•Ž˜œȱ꜑ŽœȱŒ˜—œ’žŽȱ‘Žȱ•Š›ŽœȱŠ—ȱ–˜œȱ ŸŽ›Ž‹›ŠŽœȱǻ꜑ŽœǰȱŠ–™‘’‹’Š—œǰȱŠ—ȱ›Ž™’•ŽœǼǯȱ ’ŸŽ›œŽȱŒ•Šœœȱ˜ȱŸŽ›Ž‹›ŠŽœǰȱ ’‘ȱ˜ŸŽ›ȱŘŖǰŖŖŖȱ ž››Ž—•¢ǰȱ‘ŽȱŒ•Šœœ’ęŒŠ’˜—ȱ˜ȱ꜑ȱ—Ž˜™•Šœ–œȱ known species. Their diversity and placement in is largely based on mammalian criteria (Ma- the phylogenetic tree make them ideal subjects sahito et al., 1998; Romano and Marozzi, 2004; ˜›ȱŒ˜–™Š›Š’ŸŽȱŒŠ›Œ’—˜Ž—Žœ’œȱœž’Žœǰȱ™›˜Ÿ’- Romano et al., 2010). ing broader knowledge about basic mecha- nisms, in comparison to studies limited only to ••ȱ‘Žȱ–Š“˜›ȱ›˜ž™œȱ˜ȱž–˜ž›œȱ‘Šȱ˜ŒŒž›ȱ mammalian models. Previously, such studies in mammals and birds have been reported in have primarily dealt with tumors in the higher ꜑Žœǯȱ˜–ŽȱŸŽ›¢ȱœ™ŽŒ’ęŒǰȱ Ž••Ȭ˜Œž–Ž—Žȱ vertebrates (mammals and bird); considerably —Ž˜™•Šœ’Šœȱ‘ŠŸŽȱ‹ŽŽ—ȱŽœŒ›’‹Žȱ’—ȱ꜑ǰȱ’—Œ•ž- •ŽœœȱŠĴŽ—’˜—ȱ‘Šœȱ‹ŽŽ—ȱŽŸ˜Žȱ˜ȱ‘Žȱ•˜ Ž›ȱ ing viral-induced dermal sarcomas in walleye,

* Corresponding author’s e-mail: [email protected] 26, Bull. Eur. Ass. Fish Pathol., 34(1) 2014

Stizostedion vitreumȱǻ’Œ‘’••ǼǰȱŠ—ȱŠĚŠ˜¡’—Ȭ in 50 mL H2O2c. Counterstaining was then associated hepatocellular adenomas and carci- ™Ž›˜›–Žȱ ’‘ȱ‘ŠŽ–Š˜¡¢•’—ȱǻ œžȱŽȱŠ•ǯǰȱŗşŞŗǼǯ nomas in rainbow trout, —Œ˜›‘¢—Œ‘žœȱ–¢”’œœ ǻŠ•‹Šž–Ǽǰȱ’—ȱŠ’’˜—ȱ˜ȱŠȱ ’ŽȱŸŠ›’Ž¢ȱ˜ȱ Results sporadic tumours in numerous other species The tumour was cystic and multilobulat- ǻŽŽȱŽȱŠ•ǯǰȱŗşŝŗDzȱ˜‹Ž›œǰȱŘŖŗŘǼǯ ed with abundant mucins (Figure 1). Histo- pathological examination showed the tumour Materials and methods –ŠœœȱŒ˜—Š’—ŽȱŠ›ŽŠœȱ˜ȱ–Šž›ŽȱŒ¢œ’Œȱ’œœžŽȱ Massive abdominal enlargements were ob- that exhibited typical tall columnar cells with œŽ›ŸŽȱ’—ȱŠ—ȱŠž•ȱ˜•ęœ‘ǰȱCarassius auratus basally situated nuclei and abundant intracellu- ǻǯǼȱ›˜–ȱŠ—ȱ˜›—Š–Ž—Š•ȱ꜑ȱ‹›ŽŽŽ›ȱǻŠ—Šȱ lar mucins stained with Alcian blue (Figure 2). Žǰȱ›Ž—’—ŠǼǯȱ‘Žȱ꜑ǰȱŠ‹˜žȱ ˜ȱ¢ŽŠ›œȱ˜ȱ ŽŒ˜—Š›¢ȱŒ¢œœȱŠ—ȱœ‘˜›ȱ™Š™’••Š›¢ȱ’—˜•’—œȱ ŠŽǰȱ‘ŠȱŠȱ Ž’‘ȱ˜ȱŚŜŖȱȱŠ—ȱŠȱ˜Š•ȱ•Ž—‘ȱ Ž›ŽȱŒ‘Š›ŠŒŽ›’œ’Œǯȱ —ȱœ˜–ŽȱŒ¢œœǰȱŠȱꕒ›ŽŽȱ˜ȱ ˜ȱřŚřȱ––ǯȱȱ—ŽŒ›˜™œ¢ǰȱŠȱž–˜ž›ȱ˜ȱŗşȱŒ–ȱ’—ȱ ꗎȱ™›˜“ŽŒ’˜—œȱœž™™˜›Žȱ‹¢ȱŽ•’ŒŠŽȱŒ˜——ŽŒ- ’Š–ŽŽ›ȱ Šœȱ˜ž—ȱ’—ȱ‘ŽȱŠ‹˜–’—Š•ȱŒŠŸ’¢ǯȱ ’ŸŽȱ’œœžŽȱ Šœȱ‘Žȱ™›Ž˜–’—Š—ȱ™ŠĴŽ›—ǯ ›Š–Ž—œȱ˜ȱ‘Žȱž–˜ž›ȱ Ž›Žȱę¡Žȱ’—ȱŗŖƖȱ ‹žěŽ›Žȱ˜›–Š•’—ǰȱŽ–‹ŽŽȱ’—ȱ™Š›ŠĜ—ǰȱœŽŒ- A transitional zone was observed between the tioned at 3 to 5 μm, and stained with haema- classical mucinous cystadenoma and papillary toxylin and eosin and alcian blue. In addition Š›ŽŠœǯȱŠ‘Ž›ȱ‘Š—ȱŠȱœ’—•Žȱ•Š¢Ž›ȱ˜ȱ Ž••Ȭ’쎛- histological sections were also stained using entiated mucinous cells, the cysts were lined by immunohistochemical procedures, according Š¢™’ŒŠ•ȱŽ™’‘Ž•’ž–ȱ‘Šȱ Šœȱœ›Š’ꮍȱ’—˜ȱ ˜ȱ ˜ȱŠȱ–˜’ꮍȱŠŸ’’—Ȭ‹’˜’—Ȭ™Ž›˜¡’ŠœŽȱŒ˜–™•Ž¡ȱ ˜ȱ‘›ŽŽȱ•Š¢Ž›œǯȱŽŒ’˜—œȱ˜ȱ‘Žȱ’œœžŽȱœ‘˜ Žȱ technique.2 This involved tissue slides being Š—ȱ’—ŸŠœ’ŸŽȱœ›˜–Š•ȱ’–™•Š—ȱ˜ȱŠȱ‹˜›Ž›•’—Žȱ Ž™Š›ŠĜ—’œŽǰȱ›’—œŽȱ ’‘ȱ¡¢•˜•ȱŠ—ȱ‘Ž—ȱ›Ž- mucinous tumour associated with clusters ‘¢›ŠŽȱ ’‘ȱŠ•Œ˜‘˜•œȱ˜ȱ’쎛Ž—ȱ›ŠŽœȱǻŠ‹- ˜ȱŠŽ—˜ŒŠ›Œ’—˜–ŠȬ•’”ŽȱŒŽ••œǯȱ‘Žȱ—Ž˜™•Šœ’Œȱ œ˜•žŽȱŽ‘Š—˜•ǰȱşŖǰȱŞŖǰȱŝŖǰȱśŖƖǼǯȱ‘ŽȱŽ—˜Ž—˜žœȱ epithelial structures were prominent and the peroxidase activity was blocked by incubating ’œœžŽȱ–Šœœȱ’œȱ—˜ȱ™Ž›˜›ŠŽȱǻ’ž›ŽœȱřȱŠ—ȱŚǼǯ ‘Žȱœ•’Žœȱ˜›ȱŘŖȱ–’—ȱ’—ȱŖǯřƖȱ ŘŘȱ’—ȱŠȱśƖȱ The immunohistochemical studies showed –Ž‘Š—˜•ȱœ˜•ž’˜—ǯȱŽ›ȱ Šœ‘’—ȱ‘Žȱœ•’Žœȱ’—ȱ ‘Žȱ¢™’ŒŠ•ȱ’––ž—˜‘’œ˜Œ‘Ž–’ŒŠ•ȱ™ŠĴŽ›—ȱ˜ȱ ŠŽ›ȱŠ—ȱȦŖǯŖśƖȬ ŽŽ—ȱŘŖȱœ˜•ž’˜—ǰȱ‘Ž¢ȱ œŠ’—’—ȱ˜ȱ ŝȱ˜ž—ȱ’—ȱŒ•Šœœ’ŒŠ•ȱ–žŒ’—˜žœȱŒ¢œ- Ž›Žȱ’—Œž‹ŠŽȱ’—ȱ—˜›–Š•ȱŗȦŗŖŖȱœŽ›ž–a, in a tadenoma. The invasive stromal implant also 10% PBS bovine serum albumin (BSA) solution œ‘˜ Žȱ™˜œ’’ŸŽȱœŠ’—’—ȱ ’‘ȱ‘Žȱ ŝȱŠ—’‹˜¢ȱ Šȱ—˜›–Š•ȱŽ–™Ž›Šž›Žȱ˜›ȱřŖȱ–’—ȱ’—ȱŠȱ‘ž–’ȱ (Figure 5). Œ‘Š–‹Ž›ǯȱŽ›ȱ’—Œž‹Š’˜—ǰȱ‘Žȱ™›’–Š›¢ȱŠ—’ȬȱŒ¢- ˜”Ž›Š’—ȱŝȱǻ ŝǼȱŠ—’‹˜¢b was added at a Discussion ’•ž’˜—ȱ˜ȱŗDZŘŖŖȱ’—ȱȬȱŗŖƖǰȱŠ—ȱ‘Žȱœ•’Žœȱ Ž˜™•Šœ–œȱ ˜ȱ ˜•ęœ‘ȱ Š—ȱ Œ˜––˜—ȱ ŒŠ›™ǰȱ were incubated overnight at 41°C in humid Cyprinus carpio L., are well represented in the Œ‘Š–‹Ž›ǯȱ‘’œȱ Šœȱ˜••˜ Žȱ‹¢ȱ›’—œ’—ȱŠ—ȱ •’Ž›Šž›Žǯȱ—ȱ’–™˜›Š—ȱŒ‘Š›ŠŒŽ›’œ’Œȱ˜ȱ‘’œȱ ’—Œž‹Š’˜—ȱ˜›ȱŝȱ–’—ȱ’—ȱŠȱśŖȱ–ȱřŖǯřȬ’Š–’—- ›˜ž™ȱ˜ȱ꜑ȱ’œȱ‘Ž’›ȱ•˜—ŽŸ’¢ǰȱŽœ™ŽŒ’Š••¢ȱ‘Žȱ”˜’ȱ obenzidine solution containing 1% PBS-BSA œ›Š’—œȱ˜ȱŒ˜––˜—ȱŒŠ›™ǯȱ ˜•ęœ‘ȱŠ›ŽȱŠ•œ˜ȱ Ž••ȱ ž••ǯȱž›ǯȱœœǯȱ’œ‘ȱŠ‘˜•ǯǰȱřŚǻŗǼȱŘŖŗŚǰȱŘŝ

Figure 1.ȱ ›˜œœȱŠ™™ŽŠ›Š—ŒŽȱ˜ȱ‹˜›Ž›•’—Žȱ–žŒ’—˜žœȱŒ¢œŠŽ—˜–Šȱ˜ȱŠȱ–ž•’•˜Œž•Š›ȱŒ¢œ’Œȱ˜ŸŠ›¢ȱ ’‘ȱ’››Žž•Š›ȱ walls with abundant mucins. Bar = 1 cm. Insert: Carassius auratus with massive abdominal enlargements. Bar = 4.5 cm

Figure 2. Mature cyst showing typical tall columnar cells with basally situated nuclei and abundant intracellular mucins (arrow) supported connective tissue (SC). Haematoxylin and eosin. Bar = 200 μm 28, Bull. Eur. Ass. Fish Pathol., 34(1) 2014

Figure 3.ȱ’Œ›˜œŒ˜™’ŒȱŠ™™ŽŠ›Š—ŒŽȱ˜ȱ‹˜›Ž›•’—Žȱ–žŒ’—˜žœȱŒ¢œŠŽ—˜–Šȱ˜ȱ‘Žȱ ˜ŸŠ›¢ǯȱ ™’‘Ž•’ž–ȱ ’œȱ ™•Ž˜–˜›™‘’ŒȱŠ—ȱ˜›–œȱœ–Š••ȱ™Š™’••Š›¢ȱ™›˜ŒŽœœŽœȱ ’‘ȱ’—Œ’™’Ž—ȱ’—ŸŠœ’˜—ȱ˜ȱœ›˜–ŠȱǻŠ››˜ œǼǯȱ ŠŽ–Š˜¡¢•’—ȱ and eosin. Bar = 50 μm

Figure 4.ȱ˜›Ž›•’—Žȱ–žŒ’—˜žœȱŒ¢œŠŽ—˜–Šȱ˜ȱ‘Žȱ˜ŸŠ›¢ȱœ‘˜ ’—ȱŠ¢™’ŒŠ•ȱŠ—ȱ‘¢™Ž›™•Šœ’ŒȱŽ™’‘Ž•’ž–ȱ ǻŠ››˜ œǼȱŠ—ȱ’—ŸŠœ’˜—ȱ˜ȱœ›˜–Šȱ‹¢ȱŒ•žœŽ›œȱ˜ȱŠŽ—˜ŒŠ›Œ’—˜–ŠȱŒŽ••œȱǻȘǼȱ ŠŽ–Š˜¡¢•’—ȱŠ—ȱŽ˜œ’—ǯȱŠ›ȱƽȱŗŖŖȱ μm Bull. Eur. Ass. Fish Pathol., 34(1) 2014, 29

Figure 5.ȱ›˜–Šȱ ’‘ȱŒ•žœŽ›œȱ˜ȱŠŽ—˜ŒŠ›Œ’—˜–ŠȱŒŽ••œȱŽ¡‘’‹’’—ȱ ŝȱŽ¡™›Žœœ’˜—ȱǻŠ››˜ œǼǯȱ—’Ȭ ŝȱ ’‘ȱ haematoxylin counterstain. Bar = 100 μm

adapted to environments that are suboptimal or embryonic carcinomas. Squamous cell nests ˜›ȱ˜‘Ž›ȱœ™ŽŒ’Žœȱ˜ȱ꜑ǯȱȱŒ˜—œŽšžŽ—ŒŽȱ˜ȱ‘’œȱ Ž›Žȱ˜ž—ȱ ’‘ȱ–ŽœŽ—Œ‘¢–Š•ȱŽ•Ž–Ž—œȱ’—ȱ˜—Žȱ ŠŠ™Š’˜—ȱ’œȱ‘ŽȱŠ‹’•’¢ȱ˜ȱŽ˜¡’¢ȱŠ—ȱ›Ž™Š’›ȱ tumor, but no mucinous cystadenoma was ob- damage caused by toxins that may be carcino- served. Thus, ovarian tumours in carp may have Ž—’Œǯȱ‘Žȱ•˜—ŽŸ’¢ȱ˜ȱ‘Žȱœ™ŽŒ’ŽœȱŠ—ȱ‘’œȱŽěŽŒȱ diverse histogenetic origins and most likely ˜ȱ˜¡’ŒȱŒŠ›Œ’—˜Ž—œȱ–Š¢ȱŽ¡™•Š’—ȱ‘Žȱœ’—’ęŒŠ—ȱ Š›’œŽȱ‹˜‘ȱ›˜–ȱŽ›–ȱŒŽ••œȱŠ—ȱ›˜–ȱ˜ŸŠ›’Š—ȱ —ž–‹Ž›ȱ˜ȱž–˜›œȱŽœŒ›’‹Žȱ’—ȱ‘ŽœŽȱŠ—’–Š•œǯ mesenchyme.

Abdominal enlargement associated with ovarian —ȱ‘ž–Š—ȱ™Š‘˜•˜¢ǰȱ‘Žȱ’쎛Ž—’Š’˜—ȱ˜ȱ–ŽŠ- neoplasia was previously described in eight œŠ’Œȱ–žŒ’—˜žœȱŒŠ›Œ’—˜–Šœȱ’—ȱ‘Žȱ˜ŸŠ›¢ȱ›˜–ȱ 4-6-year-old adult carp living in breeding ponds primary ovarian mucinous tumours (atypical ˜ȱŒŠ›™ȱ꜑Ž›’Žœȱ’—ȱ—˜›‘Ž›—ȱ Š™Š—ȱǻ Š›ǰȱŘŖŖśǼǯȱ ™›˜•’Ž›Š’ŸŽȦ‹˜›Ž›•’—ŽȱŠ—ȱŒŠ›Œ’—˜–ŠǼȱŒŠ—ȱ‹Žȱ ‘ŽœŽȱ—Ž˜™•Šœ’Šœȱ Ž›ŽȱŽ—Š’ŸŽ•¢ȱŒ•Šœœ’ꮍȱŠœȱ ’ĜŒž•ȱ‹ŽŒŠžœŽȱ˜ȱ‘Ž’›ȱœ’–’•Š›’’Žœȱ’—ȱ–˜›- ¢œ˜—˜Ž—Ž’Œȱž–˜ž›œȱ˜ȱ‘Žȱ˜ŸŠ›¢ǰȱ™˜œœ’- phology. bly teratoid in nature. Another study described 21 ovarian neoplasias in ornamental hybrid ˜›Ž›•’—ŽȱŒ¢œŠŽ—˜–Šœȱ˜ȱ‘Žȱ˜ŸŠ›¢ȱŠ›Žȱ˜ȱ ŒŠ›™ȱǻ œ‘’”Š ŠȱŠ—ȱŠ”Š¢Š–ŠǰȱŗşŝŞǼǯ Histologi- epithelial origin and represent a unique inter- cally, these neoplasias exhibited considerable mediate stage between benign cystadenomas ŸŠ›’Š’˜—ǰȱ‹žȱ‘Ž¢ȱ Ž›ŽȱŒ˜–™˜œŽȱ–Š’—•¢ȱ˜ȱ Š—ȱŠŽ—˜ŒŠ›Œ’—˜–Šœǯȱ‘Ž¢ȱŠ›ŽȱœŽ™Š›ŠŽȱ›˜–ȱ ŸŠ›’˜žœȱ¢™Žœȱ˜ȱŒŽ••œȱœ’–’•Š›ȱ˜ȱ‘˜œŽȱ˜ȱ‘ž–Š—ȱ Œ¢œŠŽ—˜–Šœȱ‹¢ȱ‘Žȱ™›ŽœŽ—ŒŽȱ˜ȱŒŽ••ž•Š›ȱŠ¢™’Šȱ dysgerminoma, granulosa or theca cell tumors Š—ȱ›˜–ȱ‘’‘Ȭ›ŠŽȱ–Š•’—Š—ȱž–˜›œȱ‹¢ȱ‘Žȱ 30, Bull. Eur. Ass. Fish Pathol., 34(1) 2014

™›ŽœŽ—ŒŽȱ˜ȱŽœ›žŒ’ŸŽȱœ›˜–Š•ȱ’—ŸŠœ’˜—ǯȱ˜–Žȱ žŒ’Ÿ’¢ȱ›ŽœŽŠ›Œ‘ȱŽ••˜ œ‘’™œȱ›˜–ȱ‘Žȱ›Š£’•- ‹˜›Ž›•’—ŽȱŒ¢œŠŽ—˜–Šœȱ‘ŠŸŽȱŠȱ–’—˜›ȱ˜›–ȱ ’Š—ȱ˜ž—Œ’•ȱ˜ȱŽœŽŠ›Œ‘ǰȱšȱǻ›˜ŒŽœœȱ—ž–‹Ž›ȱ ˜ȱ’—ŸŠœ’˜—ȱŽœ’—ŠŽȱŠœȱ–’Œ›˜’—ŸŠœ’˜—ȱǻŒœǰȱ ȱřŖŗŖŖŘȦŘŖŗŘȬŜȱŠ—ȱřŖŞŖŗřȦŘŖŖşȬřǼǯȱ 2005). References The World Health Organization (WHO) classi- Acs G (2005). Serous and mucinous borderline ǻ•˜ ȱ–Š•’—Š—ȱ™˜Ž—’Š•Ǽȱž–˜›œȱ˜ȱ‘Žȱ ꎍȱ‘ŽœŽȱ—Ž˜™•Šœ–œȱ’—ȱ‘ž–Š—œȱŠœȱȃ‹˜›Ž›•’—Žȱ ovary. American Journal of Clinical Pathology tumours”; they have been too widely recognized 123, ŗřȬśŝǯ Šœȱ˜ŸŠ›’Š—ȱž–˜›œȱ˜ȱȃ•˜ ȱ–Š•’—Š—ȱ™˜Ž—’Š•Ȅȱ ›’ĵȱǰȱ Ž›ŸŽ¢ȱ ǰȱ ŠŒ”ȱǰȱ ŠŠœȱ ǰȱ ˜››Žœȱ Š—ȱŠœȱȃ™›˜•’Ž›Š’ŸŽȱ˜ŸŠ›’Š—ȱž–˜ž›œǰȄȱŽ›–’- MA and Burtom KL (2000). International nology that was accepted by the WHO in the Œ•Šœœ’ęŒŠ’˜—ȱ˜ȱ‘Žȱ’œŽŠœŽœȱ˜›ȱ˜—Œ˜•˜¢ǰȱ ¢ŽŠ›ȱŘŖŖŖǯȱž››Ž—•¢ǰȱ‘ŽȱžœŽȱ˜ȱ‘ŽȱŽœ’—Š’˜—ȱ 3th ed, World Health Organization (WHO), Geneva. ȃŠŽ—˜–Šȱ˜›ȱŒ¢œŠŽ—˜–Šȱ˜ȱ•˜ ȱ–Š•’—Š—ȱ™˜- Ž—’Š•Ȅȱ’œȱ—˜ȱ›ŽŒ˜––Ž—Žȱǻ›’ĵǰȱŽȱŠ•ǯǰȱŘŖŖŖǼǯȱ Hart WR (2005). Borderline epithelial tumors ˜ȱ‘Žȱ˜ŸŠ›¢ǯȱModern Pathology 18, 33–50.

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