Bull. Eur. Ass. Fish Pathol., 34(1) 2014, 25 Borderline ovarian mucinous cystadenoma with ȱȱȱȱȱęȱ Carassius auratus (L.) L. A. Romano1Șǰȱǯȱ ě2ǰȱǯȱû2, R. V. Rodrigues3, M. B. Tesser3, M. A. R Garrido-Pereira1 and L. A. Sampaio3 1ȱàȱȱ ȱȱȱȱȱǰȱ ȱȱęǰȱȱ ȱȱȱ ǯȱ¡ȱȱŚŝŚǰȱȱşŜǯŘŖŗȬşŖŖǰȱȱ ǰȱǰȱDzȱ2 Programa de Pós- ³¨ȱȱǰȱȱȱȱȱ ǯȱ¡ȱȱŚŝŚǰȱȱşŜŘŖŗȬşŖŖǰȱ ȱ ǰȱǰȱDzȱ3ȱàȱȱȱȱȱǰȱ ȱȱęǰȱ ȱȱȱȱ ǯȱ¡ȱȱŚŝŚǰȱȱşŜŘŖŗȬşŖŖǰȱȱ ǰȱǰȱ Abstract ȱȱȱȱ¢ȱȱȱȱęǯȱȱȱȱ¡ȱ ȱȱȱȱȱȱȱȱ¢ȱȱȱ¡ȱ¢ȱȱȱȱ with basally situated nuclei and abundant intracellular mucins stained with Alcian blue. Sections ȱȱȱ ȱȱȱȱȱȱȱȱ¢ȱ- ȱ ȱȱȱȬȱǯȱȱȱȱ ȱȱ ¢ȱȱĴȱȱȱȱ ŝȱȱȱȱȱ¢ǯȱ ȱȱȱȱȱ ȱȱȱ ȱȱ ŝȱ¢ǯȱȱ¢ȱȱ ¢ȱŝȱǻ ŝǼȱ¡ȱȱȱȱ ȱȱȱȱȱȱě- ȱȱ ȱȱǰȱȱȱ¡ȱȱȱȱ ŝȱȱȱ ȱȱȱ ¢ȱȱȱȱȱȱ¢ȱȱ¢ȱȱȱȱȱ¢ǯ Introduction ȱęȱȱȱȱȱȱ ȱǻęǰȱǰȱȱǼǯȱ ȱȱȱǰȱ ȱȱŘŖǰŖŖŖȱ ¢ǰȱȱęȱȱęȱȱ known species. Their diversity and placement in is largely based on mammalian criteria (Ma- the phylogenetic tree make them ideal subjects sahito et al., 1998; Romano and Marozzi, 2004; ȱȱȱǰȱ- Romano et al., 2010). ing broader knowledge about basic mecha- nisms, in comparison to studies limited only to ȱȱȱȱȱȱȱȱ mammalian models. Previously, such studies in mammals and birds have been reported in have primarily dealt with tumors in the higher ęǯȱȱ¢ȱęǰȱ Ȭȱ vertebrates (mammals and bird); considerably ȱȱȱȱȱęǰȱ- ȱĴȱȱȱȱȱȱ ȱ ing viral-induced dermal sarcomas in walleye, * Corresponding author’s e-mail: [email protected] 26, Bull. Eur. Ass. Fish Pathol., 34(1) 2014 Stizostedion vitreumȱǻǼǰȱȱĚ¡Ȭ in 50 mL H2O2c. Counterstaining was then associated hepatocellular adenomas and carci- ȱ ȱ¡¢ȱǻ ȱȱǯǰȱŗşŞŗǼǯ nomas in rainbow trout, ¢ȱ¢ ǻǼǰȱȱȱȱȱ ȱ¢ȱȱ Results sporadic tumours in numerous other species The tumour was cystic and multilobulat- ǻȱȱǯǰȱŗşŝŗDzȱǰȱŘŖŗŘǼǯ ed with abundant mucins (Figure 1). Histo- pathological examination showed the tumour Materials and methods ȱȱȱȱȱ¢ȱȱ Massive abdominal enlargements were ob- that exhibited typical tall columnar cells with ȱȱȱȱęǰȱCarassius auratus basally situated nuclei and abundant intracellu- ǻǯǼȱȱȱȱęȱȱǻȱ lar mucins stained with Alcian blue (Figure 2). ǰȱǼǯȱȱęǰȱȱ ȱ¢ȱȱ ¢ȱ¢ȱȱȱ¢ȱȱ ǰȱȱȱ ȱȱŚŜŖȱȱȱȱȱȱ ȱǯȱ ȱȱ¢ǰȱȱęȱȱ ȱřŚřȱǯȱȱ¢ǰȱȱȱȱŗşȱȱȱ ęȱȱȱ¢ȱȱ- ȱ ȱȱȱȱȱ¢ǯȱ ȱȱ ȱȱȱĴǯ ȱȱȱȱ ȱę¡ȱȱŗŖƖȱ ěȱǰȱȱȱĜǰȱ- A transitional zone was observed between the tioned at 3 to 5 μm, and stained with haema- classical mucinous cystadenoma and papillary toxylin and eosin and alcian blue. In addition ǯȱȱȱȱȱ¢ȱȱ Ȭě- histological sections were also stained using entiated mucinous cells, the cysts were lined by immunohistochemical procedures, according ¢ȱȱȱ ȱęȱȱ ȱ ȱȱęȱȬȬ¡ȱ¡ȱ ȱȱ¢ǯȱȱȱȱȱ ȱ technique.2 This involved tissue slides being ȱȱȱȱȱȱȱ Ĝǰȱȱ ȱ¡¢ȱȱȱ- mucinous tumour associated with clusters ¢ȱ ȱȱȱěȱȱǻ- ȱȬȱǯȱȱȱ ȱǰȱşŖǰȱŞŖǰȱŝŖǰȱśŖƖǼǯȱȱȱ epithelial structures were prominent and the peroxidase activity was blocked by incubating ȱȱȱȱȱǻȱřȱȱŚǼǯ ȱȱȱŘŖȱȱȱŖǯřƖȱ ŘŘȱȱȱśƖȱ The immunohistochemical studies showed ȱǯȱȱ ȱȱȱȱ ȱ¢ȱȱĴȱȱ ȱȱȦŖǯŖśƖȬ ȱŘŖȱǰȱ¢ȱ ȱȱ ŝȱȱȱȱȱ¢- ȱȱȱȱŗȦŗŖŖȱa, in a tadenoma. The invasive stromal implant also 10% PBS bovine serum albumin (BSA) solution ȱȱȱ ȱȱ ŝȱ¢ȱ ȱȱȱȱřŖȱȱȱȱȱ (Figure 5). ǯȱȱǰȱȱ¢ȱȬȱ¢- ȱŝȱǻ ŝǼȱ¢b was added at a Discussion ȱȱŗDZŘŖŖȱȱȬȱŗŖƖǰȱȱȱȱ ȱ ȱ ęȱ ȱ ȱ ǰȱ were incubated overnight at 41°C in humid Cyprinus carpio L., are well represented in the ǯȱȱ ȱ ȱ¢ȱȱȱ ǯȱȱȱȱȱȱ ȱȱŝȱȱȱȱśŖȱȱřŖǯřȬ- ȱȱęȱȱȱ¢ǰȱ¢ȱȱȱ obenzidine solution containing 1% PBS-BSA ȱȱȱǯȱ ęȱȱȱ ȱ ǯȱǯȱǯȱȱǯǰȱřŚǻŗǼȱŘŖŗŚǰȱŘŝ Figure 1.ȱ ȱȱȱȱȱ¢ȱȱȱȱ¢ȱ¢ȱ ȱȱ walls with abundant mucins. Bar = 1 cm. Insert: Carassius auratus with massive abdominal enlargements. Bar = 4.5 cm Figure 2. Mature cyst showing typical tall columnar cells with basally situated nuclei and abundant intracellular mucins (arrow) supported connective tissue (SC). Haematoxylin and eosin. Bar = 200 μm 28, Bull. Eur. Ass. Fish Pathol., 34(1) 2014 Figure 3.ȱȱȱȱȱȱ¢ȱȱȱ ¢ǯȱ ȱ ȱ ȱȱȱȱ¢ȱȱ ȱȱȱȱȱǻ Ǽǯȱ ¡¢ȱ and eosin. Bar = 50 μm Figure 4.ȱȱȱ¢ȱȱȱ¢ȱ ȱ¢ȱȱ¢ȱȱ ǻ Ǽȱȱȱȱȱ¢ȱȱȱȱȱǻȘǼȱ ¡¢ȱȱǯȱȱƽȱŗŖŖȱ μm Bull. Eur. Ass. Fish Pathol., 34(1) 2014, 29 Figure 5.ȱȱ ȱȱȱȱȱ¡ȱ ŝȱ¡ȱǻ ǼǯȱȬ ŝȱ ȱ haematoxylin counterstain. Bar = 100 μm adapted to environments that are suboptimal or embryonic carcinomas. Squamous cell nests ȱȱȱȱęǯȱȱȱȱȱ ȱȱ ȱ¢ȱȱȱȱ ȱȱȱ¢ȱȱ¡¢ȱȱȱ tumor, but no mucinous cystadenoma was ob- damage caused by toxins that may be carcino- served. Thus, ovarian tumours in carp may have ǯȱȱ¢ȱȱȱȱȱȱěȱ diverse histogenetic origins and most likely ȱ¡ȱȱ¢ȱ¡ȱȱęȱ ȱȱȱȱȱȱȱȱ ȱȱȱȱȱȱǯ mesenchyme. Abdominal enlargement associated with ovarian ȱȱ¢ǰȱȱěȱȱ- neoplasia was previously described in eight ȱȱȱȱȱ¢ȱȱ 4-6-year-old adult carp living in breeding ponds primary ovarian mucinous tumours (atypical ȱȱęȱȱȱ ȱǻ ǰȱŘŖŖśǼǯȱ ȦȱȱǼȱȱȱ ȱȱ ȱ¢ȱęȱȱ Ĝȱȱȱȱȱȱ- ¢ȱȱȱȱ¢ǰȱ- phology. bly teratoid in nature. Another study described 21 ovarian neoplasias in ornamental hybrid ȱ¢ȱȱȱ¢ȱȱȱ ȱǻ ȱȱ¢ǰȱŗşŝŞǼǯ Histologi- epithelial origin and represent a unique inter- cally, these neoplasias exhibited considerable mediate stage between benign cystadenomas ǰȱȱ¢ȱ ȱȱ¢ȱȱ ȱǯȱ¢ȱȱȱȱ ȱ¢ȱȱȱȱȱȱȱȱ ¢ȱ¢ȱȱȱȱȱ¢ȱ dysgerminoma, granulosa or theca cell tumors ȱȱȬȱȱȱ¢ȱȱ 30, Bull. Eur. Ass. Fish Pathol., 34(1) 2014 ȱȱȱȱǯȱȱ ¢ȱȱ ȱȱȱ£- ȱ¢ȱȱȱȱȱ ȱȱȱǰȱȱǻȱȱ ȱȱȱȱȱǻǰȱ ȱřŖŗŖŖŘȦŘŖŗŘȬŜȱȱřŖŞŖŗřȦŘŖŖşȬřǼǯȱ 2005). References The World Health Organization (WHO) classi- Acs G (2005). Serous and mucinous borderline ǻ ȱȱǼȱȱȱȱ ęȱȱȱȱȱȱȃȱ ovary. American Journal of Clinical Pathology tumours”; they have been too widely recognized 123, ŗřȬśŝǯ ȱȱȱȱȃ ȱȱȄȱ ĵȱǰȱ ¢ȱ ǰȱ ȱǰȱ ȱ ǰȱ ȱ ȱȱȃȱȱǰȄȱ- MA and Burtom KL (2000). International nology that was accepted by the WHO in the ęȱȱȱȱȱ¢ǰȱ ¢ȱŘŖŖŖǯȱ¢ǰȱȱȱȱȱȱ 3th ed, World Health Organization (WHO), Geneva. ȃȱȱ¢ȱȱ ȱȱ- ȄȱȱȱȱǻĵǰȱȱǯǰȱŘŖŖŖǼǯȱ Hart WR (2005). Borderline epithelial tumors ȱȱ¢ǯȱModern Pathology 18, 33–50. ȱȱȱȱĚȱȱ Hsu SM, Raine L and Fanger H (1981). Use ȱ ȬȬ¡ȱ ¡ȱ ȱ ȱ ȱ ȱ ěȱ ȱ (ABC) in immunoperoxidase techniques: primary ovarian mucinous tumours and meta- a comparison between ABC an unlabeled static mucinous carcinomas in the human ovary. antibody (PAP) procedures. Journal of ȱ¢ȱȱ¢ȱŝȱǻ ŝǼȱ¡ȱȱ Histochemistry & Cytochemistry 29ǰȱśŝŝȮśŞśǯ restricted and allows ovarian mucinous tumors ȱȱȱ¢ȱȱǻŗşŝŞǼǯȱȱ ȱȱěȱȱ ȱȱ neoplasia in ornamental hybrid carp ǰȱȱȱ¡ȱȱȱȱ ŝȱȱ (Nishikigoi) in Japan. ȱȱȱ ȱ ȱ¢ȱȱ. 298, 330–341. ȱ ȱȱȱ¢ȱȱȱȱ ȱȱ¢ȱȱ¢ȱ- Lee DL, Wales JH and Sinnhuber RO ǻŗşŝŗǯȱ ȱ ȱȱȱȱ¢ǯȱ¢ȱȱ ȱĚ¡ȱȱȱ ȱȱ ȱȱ ȱ¢ȱ ¢ȱ- trout by methyl-malvalate and sterculate. ¢ȱȱȱ ȱŝȱǻşŞƖǼȱǻȱ Cancer Research 31, 960–963. 2011). Ovarian neoplasms have been reported in Masahito P, Ishikawa T and Sugano H (1998). ęǰȱȱȱȱ¢ȱȱ Fish tumors and their importance in cancer ȱ¢ȱ ȱȱȱȱȱǰȱ research. Japanese Journal of Cancer Research 79, 545–555. ȱȱ ȱȱȱȱȱęȱȱ case (Hart, 2005). 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