Magic™ Anti-RB (Phospho S780) Monoclonal Antibody, Clone FS293(O) (DCABH-5848) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

Magic™ Anti-RB (Phospho S780) monoclonal antibody, clone FS293(O) (DCABH-5848) This product is for research use only and is not intended for diagnostic use.

PRODUCT INFORMATION

Product Overview Rabbit monoclonal to Rb (phospho S780)

Antigen Description Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 Lys-20 trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c- FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1s activity.

Specificity This antibody only detects Rb phosphorylated at serine 780.

Target RB

Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Rb aa 750-850 (phospho S780) (Cysteine residue). The exact sequence is proprietary.Database link: P06400

Isotype IgG

Source/Host Rabbit

Species Reactivity Human

Clone FS293(O)

Purity Tissue culture supernatant

Conjugate Unconjugated

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Positive Control Jurkat cell lysate; Human thyroid gland carcinoma and testis tissues.

Procedure Phospho-specific Antibodies

Format Liquid

Size 40 μl

Buffer pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA

Preservative 0.01% Sodium Azide

Storage Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Ship Shipped at 4°C.

GENE INFORMATION

Gene Name RB1 retinoblastoma 1 [ Homo sapiens ]

Official Symbol RB1

Synonyms RB1; retinoblastoma 1; OSRC, osteosarcoma; retinoblastoma-associated protein; RB; retinoblastoma suspectibility protein; pRb; OSRC; pp110; p105-Rb;

Entrez Gene ID 5925

Protein Refseq NP_000312

UniProt ID A0A024RDV3

Chromosome Location 13q14.2

Pathway Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism- specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem;

Function DNA binding; RNA polymerase II activating transcription factor binding; androgen receptor binding; core promoter binding; kinase activity; kinase binding; molecular_function; phosphoprotein binding; protein binding; sequence-specific DNA binding transcrip

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]