DOCSLIB.ORG

Injection of Incubating Chicken Eggs: Effects on Embryo Viability, Tissue Se Concentration, Lipid Peroxidation, Immune Response and Post Hatch Development

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Injection of Incubating Chicken Eggs: Effects on Embryo Viability, Tissue Se Concentration, Lipid Peroxidation, Immune Response and Post Hatch Development University of Kentucky UKnowledge Theses and Dissertations--Animal and Food Sciences Animal and Food Sciences 2012 IN OVO SELENIUM (SE) INJECTION OF INCUBATING CHICKEN EGGS: EFFECTS ON EMBRYO VIABILITY, TISSUE SE CONCENTRATION, LIPID PEROXIDATION, IMMUNE RESPONSE AND POST HATCH DEVELOPMENT Lizza M. Macalintal University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Macalintal, Lizza M., "IN OVO SELENIUM (SE) INJECTION OF INCUBATING CHICKEN EGGS: EFFECTS ON EMBRYO VIABILITY, TISSUE SE CONCENTRATION, LIPID PEROXIDATION, IMMUNE RESPONSE AND POST HATCH DEVELOPMENT" (2012). Theses and Dissertations--Animal and Food Sciences. 4. https://uknowledge.uky.edu/animalsci_etds/4 This Doctoral Dissertation is brought to you for free and open access by the Animal and Food Sciences at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Animal and Food Sciences by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained and attached hereto needed written permission statements(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine). I hereby grant to The University of Kentucky and its agents the non-exclusive license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known. I agree that the document mentioned above may be made available immediately for worldwide access unless a preapproved embargo applies. I retain all other ownership rights to the copyright of my work. I also retain the right to use in future works (such as articles or books) all or part of my work. I understand that I am free to register the copyright to my work. REVIEW, APPROVAL AND ACCEPTANCE The document mentioned above has been reviewed and accepted by the student’s advisor, on behalf of the advisory committee, and by the Director of Graduate Studies (DGS), on behalf of the program; we verify that this is the final, approved version of the student’s dissertation including all changes required by the advisory committee. The undersigned agree to abide by the statements above. Lizza M. Macalintal, Student Dr. Austin H. Cantor, Major Professor Dr. David L. Harmon, Director of Graduate Studies ABSTRACT OF DISSERTATION Lizza M. Macalintal The Graduate School University of Kentucky 2012 IN OVO SELENIUM (SE) INJECTION OF INCUBATING CHICKEN EGGS: EFFECTS ON EMBRYO VIABILITY, TISSUE SE CONCENTRATION, LIPID PEROXIDATION, IMMUNE RESPONSE AND POST HATCH DEVELOPMENT ABSTRACT OF DISSERTATION An abstract of a dissertation submitted in partial fulfillment of the requirements for the Degree of Doctor of Philosophy in the College of Agriculture at the University of Kentucky by Lizza M. Macalintal Lexington, Kentucky Director: Dr. Austin H. Cantor, Associate Professor of Animal Sciences Lexington, Kentucky 2012 Copyright © Lizza M. Macalintal 2012 ABSTRACT OF DISSERTATION IN OVO SELENIUM (SE) INJECTION OF INCUBATING CHICKEN EGGS: EFFECTS ON EMBRYO VIABILITY, TISSUE SE CONCENTRATION, LIPID PEROXIDATION, IMMUNE RESPONSE AND POST HATCH DEVELOPMENT Studies were conducted to investigate the effects of in ovo injection of selenium (Se) either as seleno-methionine (Se-Met) or sodium selenite (Na2SeO3) into the yolk of incubating eggs on tissue Se concentration, embryo livability, lipid peroxidation, immune response and growth performance. When white-shelled eggs were injected with 0.1ml of solutions providing 0, 2.5, 5, 10 or 20 μg Se/egg, no detrimental effects on embryo viability at 20 days of incubation were noted. The effects on tissue Se concentrations suggested that Se-Met and Na2SeO3 were metabolized differently by the chick embryo. In a subsequent study using injection doses up to 60 μg/egg, a greater linear response in tissue Se was obtained with Se-Met, compared with Na2SeO3 (P < 0.01). Minimal changes in heart and breast muscle Se concentrations were noted above the 40 µg dose when Na2SeO3 was used (P > 0.05). In a study with broiler eggs, injection doses of 0, 2.5, 5, 10, 20 and 40 μg Se/egg were used. Se-Met or Na2SeO3 at doses up to 40 μg Se/egg had little effect on embryo viability. Injecting Se-Met resulted in greater tissue Se accumulation than Na2SeO3 at 20 days of incubation. In another study with broiler eggs using injection doses up to 40 μg Se/egg, Se-Met injection resulted in higher hatchability, reduced lipid peroxidation in the lung and heart muscle of the embryos after 20 days incubation and higher Se concentrations in heart and breast muscle of hatched chicks through 7 days and in lung through 21 days of growth. In a feeding trial with broiler breeder hens, adding 0.3 mg/kg of Se as Se yeast or Na2SeO3 to the diet improved tissue Se status at hatching of progeny chicks. Taken together, these results indicate that injection of Se into the yolk of incubating eggs may be useful for enhancing Se status during embryonic and early post-hatch development. Therefore, the improvement in Se status using this method in conjunction with dietary Se supplementation of breeder hens would be much greater than with only using dietary supplementation. Key words: in ovo injection, selenium, embryo, lipid peroxidation, sheep red blood cell Lizza M. Macalintal __________________________________ April 26, 2012 ___________________________________ IN OVO SELENIUM INJECTION OF INCUBATING CHICKEN EGGS: EFFECTS ON EMBRYO VIABILITY, TISSUE SE CONCENTRATION, LIPID PEROXIDATION, IMMUNE RESPONSE AND POST HATCH DEVELOPMENT By Lizza M. Macalintal Dr. Austin H. Cantor Director of Dissertation Dr. David L. Harmon Director of Graduate Studies April 26, 2012 (Date) RULES FOR THE USE OF DISSERTATION Unpublished dissertations submitted for the Doctor’s degree and deposited in the University of Kentucky Library are as a rule open for inspection, but are to be used only with due regard to the rights of the authors. Bibliographical references may be noted, but quotations or summaries of parts may be published only with the permission of the author, and with the usual scholarly acknowledgements. Extensive copying or publication of the dissertation in whole or in part also requires the consent of the Dean of Graduate School of the University of Kentucky. A library that borrows this dissertation for use by its patrons is expected to secure the signature of each user. Name Date ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ DISSERTATION Lizza M. Macalintal The Graduate School University of Kentucky 2012 IN OVO SELENIUM (SE) INJECTION OF INCUBATING CHICKEN EGGS: EFFECTS ON EMBRYO VIABILITY, TISSUE SE CONCENTRATION, LIPID PEROXIDATION, IMMUNE RESPONSE AND POST HATCH DEVELOPMENT DISSERTATION An abstract of a dissertation submitted in partial fulfillment of the requirements for the Degree of Doctor of Philosophy in the College of Agriculture at the University of Kentucky by Lizza M. Macalintal Lexington, Kentucky Director: Dr. Austin H. Cantor, Associate Professor of Animal Sciences Lexington, Kentucky 2012 Copyright © Lizza M. Macalintal 2012 ACKNOWLEDGEMENT I would like to express my utmost gratitude to my major professor, Dr. Austin H. Cantor and my committee members Dr. Anthony J. Pescatore, Dr. James L. Pierce and Dr. Gilbert Boissoneault. It has been a great honor to work with all of you and I will always be in awe that I was and am mentored by such great scientists. To Dr. Cantor, for all the guidance, patience and for all the knowledge you have shared with me, to prepare to the next big thing, I cannot thank you enough for trusting in my abilities. To Dr. Pescatore, as Mark Twain has quoted “really great people make you feel that you, too, can become great” that line comes to mind when I think of how to express my gratitude to you. To Dr. James Pierce, who I first met in Manila in 2005, thank you for accepting me into the Alltech-UK PhD program, I am truly grateful. To Dr. Boissoneault, for accepting the invitation as one of my committee members, for your encouragement and assistance, my sincerest gratitude. To Dr. C.K. Chow, thank you for accepting the invitation to sit as my outside examiner. It is very seldom in our lives that we meet people who influence us in the most positive way and help us improve to further our careers, for
Load more

Recommended publications
  • A Comparative Analysis of Oocyte Development in Mammals
    cells Review A Comparative Analysis of Oocyte Development in Mammals Rozenn Dalbies-Tran 1,*, Véronique Cadoret 1,2, Alice Desmarchais 1,Sébastien Elis 1 , Virginie Maillard 1, Philippe Monget 1, Danielle Monniaux 1 , Karine Reynaud 1, Marie Saint-Dizier 1 and Svetlana Uzbekova 1 1 INRAE, CNRS, Université de Tours, IFCE, PRC, F-37380 Nouzilly, France 2 CHU Bretonneau, Médecine et Biologie de la Reproduction-CECOS, 37044 Tours, France * Correspondence: [email protected] Received: 14 February 2020; Accepted: 9 April 2020; Published: 17 April 2020 Abstract: Sexual reproduction requires the fertilization of a female gamete after it has undergone optimal development. Various aspects of oocyte development and many molecular actors in this process are shared among mammals, but phylogeny and experimental data reveal species specificities. In this chapter, we will present these common and distinctive features with a focus on three points: the shaping of the oocyte transcriptome from evolutionarily conserved and rapidly evolving genes, the control of folliculogenesis and ovulation rate by oocyte-secreted Growth and Differentiation Factor 9 and Bone Morphogenetic Protein 15, and the importance of lipid metabolism. Keywords: oocyte; mammals; evolution; gene expression; posttranscriptional control; Gdf9; Bmp15; lipids 1. Introduction Sexual reproduction requires the fertilization of a female gamete after it has undergone optimal development. In animals, oogenesis has been studied in organisms as diverse as insects, worms, amphibians and mammals. In the latter, the mouse has long been the model of choice to delineate the complex mechanisms that regulate oogenesis and to identify the major genes and molecular actors involved throughout the process.
    [Show full text]
  • Eggcyclopedia
    American Egg Board FIFTH EDITION the incredible edible egg™ EGGCYCLOPEDIA We are proud to present the newly revised, fifth edition of The Incredible Edible Egg™ Eggcyclopedia. This comprehensive, in-depth resource is designed to provide food and health professionals, as well as consumers with the latest egg information from A-Z. The Eggcyclopedia was developed by the American Egg Board (AEB) on behalf of America’s egg farmers who are committed to caring for their hens and producing a high-quality product. This commitment starts on the farms and continues through the egg’s journey to your table. 2 A A Although the air cell usually forms in the large end of the egg, it occasionally Aioli moves freely toward the uppermost Garlic mayonnaise popular in the point of the egg as the egg is rotated. Provence region of southern France. It is then called a free or floating air cell. - See Mayonnaise If the main air cell ruptures, resulting in one or more small separate air Air Cell bubbles floating beneath the main air The air-filled pocket between the white cell, it is known as a bubbly air cell. and shell at the large end of the egg. Candlers use the size of the air cell as When an egg is newly laid, it is about one basis for determining grade. 105ºF (41ºC) and has either no air cell or a very small one. As the egg cools, the liquid contents contract more than the shell and the inner shell membrane separates from the outer shell membrane to form the air cell.
    [Show full text]
  • The Amazing Science of Eggs Welcome
    A learning resource from The British Hen Welfare Trust Egg Heads The amazing science of eggs Welcome Thank you for downloading this resource pack. We hope you will find it useful. We’ve produced these resource to help your pupils explore the issues around egg production while developing new skills across the curriculum and applying them to real life situations. We love hearing from schools who have used our resources! If you have feedback, comments or suggestions that you’d like to share then please email them to [email protected] We love seeing your photos and artwork too! Using this pack... In this pack you will find a selection of lesson plans each with background notes, a resource list, and suggestions for extending the activities. Look out for the icons found throughout the pack to see what kind of activities or skills each element of the lesson plan supports. For example: Creative Sharing a Discussion writing story or debate All activity sheets, resource sheets and supporting resources can be found at the back of the pack. This is one of six resource packs. You can find the others, together with guidance on keeping your own school hens and other useful resources, on our website: www.bhwt.co.uk 2 What’s inside? Lesson plans Amazing eggs This resource is packed with messy, tasty, arty and Page 4 For EYFS to KS2 creative ideas for using eggs to explore the science curriculum. Eggs are everywhere! In this lesson pupils learn which foods are made with Page 13 For KS1 egg, the role eggs play in different kinds of cookery, and how to tell if the eggs they are using were laid by happy hens.
    [Show full text]
  • DATCP Docket No. 13-R-05 Rules Clearinghouse No. 14-037 ORDER
    DATCP Docket No. 13-R-05 Rules Clearinghouse No. 14-037 ORDER OF THE WISCONSIN DEPARTMENT OF AGRICULTURE, TRADE AND CONSUMER PROTECTION ADOPTING RULES 1 The Wisconsin department of agriculture, trade and consumer protection hereby adopts the following rule 2 to amend ATCP 75 (appendix) (3-202.13); to repeal and recreate ATCP 88; and to create ATCP 70.02 3 (16) (h) and (i), 70.04 (17), 70.06 (11), 70.09 (8), 70.10(6), and ATCP 75 (appendix) (3-201.11) (H); 4 relating to egg grading, handling and labeling, and affecting small business. _____________________________________________________________________________ Analysis Prepared by the Department of Agriculture, Trade and Consumer Protection This rule modifies: ch. ATCP 88, Wis. Adm. Code, related to egg grading, handling and labeling; ch. ATCP 70, Wis. Adm. Code, related to food processing plants; and, ch. ATCP 75, Wis. Adm. Code, related to retail food establishments. The rule comprehensively revises ATCP 88 to clarify the regulatory requirements applicable to egg producers and egg handlers. The rule makes minor revisions to chs. ATCP 70 and 75, moving primary egg regulation to ATCP 88 and further defining acceptable sources of eggs to be sold in retail food establishments. By placing requirements for licensing, facilities, equipment and utensils, egg handling operations, packing and labeling, recordkeeping and recall planning in ATCP 88, the rule limits the need for small egg-business operators to consult multiple chapters of rules. The rule implements 2013 Wisconsin Act 245 by eliminating the requirement for small-scale egg producers to hold a food processing plant license when selling eggs to consumers at a farmers’ market, on an egg sales route, or at the egg producer’s farm.
    [Show full text]
  • Chapter ATCP 88
    Published under s. 35.93, Wis. Stats., by the Legislative Reference Bureau. 663 AGRICULTURE, TRADE AND CONSUMER PROTECTION ATCP 88.01 Chapter ATCP 88 EGGS Subchapter I — General Provisions ATCP 88.20 Egg cleaning and storage operations. ATCP 88.01 Definitions. ATCP 88.22 Candling; candling requirements. ATCP 88.02 Licensing and Registration. ATCP 88.24 Grading standards for chicken eggs. ATCP 88.04 Federal registrations and records. ATCP 88.26 Minimum tolerance standards. ATCP 88.28 Restricted eggs. Subchapter II — Egg Facilities ATCP 88.30 Shell egg protection; egg shell oil. ATCP 88.06 Egg handling and storage facilities. ATCP 88.08 Egg handling rooms. Subchapter V — Packing and Labeling ATCP 88.10 Operations water. ATCP 88.32 Egg packing. ATCP 88.34 Egg labeling. Subchapter III — Equipment and Utensils ATCP 88.36 Labeling of baluts. ATCP 88.12 Equipment and utensil requirements. ATCP 88.38 Deceptive practices. ATCP 88.14 Cleaning and sanitizing equipment and utensils. Subchapter VI — Recordkeeping, Recall Planning, and Enforcement Subchapter IV — Egg Handling Operations ATCP 88.40 Dealers buying eggs from producers; receipts. ATCP 88.16 Personnel standards. ATCP 88.42 Recall plan. ATCP 88.18 Temperature standards. ATCP 88.44 Enforcement. History: Chapter Ag 90 as it existed on June 30, 1974 was repealed and a new (12) “Egg sales route” means one or more residences inhab- chapter Ag 90 created effective July 1, 1974; Chapter Ag 90 was renumbered ch. ATCP 88 under s. 13.93 (2m) (b) 1., Stats., Register, April, 1993, No. 448. Chapter ited by consumers who regularly buy eggs from an egg producer ATCP 88 as it existed on October 31, 1996 was repealed and a new chapter ATCP traveling to the residences.
    [Show full text]
  • Avian Embryo
    Avian Embryo The avian embryo is amazing and exciting. In only three weeks, a small clump of cells with no characteristic features of any single animal species changes into an active, newly hatched chick. A study of this transformation is educational and interesting, and gives us insight into how humans are formed. This publication will help you study the formation of the egg and the avian embryo. It includes plans for two small incubators so you can build one. You can buy small commercially-built incubators at stores selling farm and educational supplies. Incubation procedures show you the effects of heat, moisture, and ventilation upon the development of the chick embryo. You also learn to hatch other fowl such as turkeys, ducks, quail, and pheasants. The publication describes how to observe and exhibit an avian embryo while it is alive and still functioning, or as a preserved specimen. Sections of this publication include: • Formation and Parts of the Egg • Stages of Embryonic Development • Incubating and Hatching Chicks • Constructing an Egg Incubator • Embryology Projects Formation and Parts of the Egg The avian egg, in all its complexity, is still a mystery. A highly complex reproductive cell, it is essentially a tiny center of life. Initial development of the embryo takes place in the blastoderm. The albumen surrounds the yolk and protects this potential life. It is an elastic, shock-absorbing semi-solid with a high water content. Together, the yolk and albumen are prepared to sustain life - the life of a growing embryo - for three weeks, in the case of the chicken.
    [Show full text]
  • Offspring Sex Ratio Bias and Sex Related Characteristics of Eggs in Chicken, 192 Pages
    Offspring sex ratio bias and sex related characteristics of eggs in chicken Muhammad Aamir Aslam Thesis committee Promotors Prof. Dr M.A. Smits Professor of Intestinal Health of Animals Wageningen University Prof. Dr T.G.G. Groothuis Professor of Behavioural biology University of Groningen Co-promotor Dr H. Woelders Senior Research Scientist, Animal Breeding and Genomics Centre Wageningen UR Livestock Research Other members Prof. Dr M.E. Visser, Wageningen University Dr F.R Leenstra, Wageningen UR Livestock Research Dr V. Goerlich-Jansson, Bielefeld University, Germany Dr K.J. Navara, The University of Georgia, Athens, USA This research was conducted under the auspices of the Graduate School of Wageningen Institute of Animal Sciences (WIAS) Offspring sex ratio bias and sex related characteristics of eggs in chicken Muhammad Aamir Aslam Thesis submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr M.J. Kropff, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Monday 8 September 2014 at 11 a.m. in the Aula. Muhammad Aamir Aslam Offspring sex ratio bias and sex related characteristics of eggs in chicken, 192 pages. PhD thesis, Wageningen University, Wageningen, NL (2014) With references, with summaries in Dutch and English ISBN 978-94-6257-075-7 Abstract Aslam, M.A. (2014), Offspring sex ratio bias and sex related characteristics of eggs in chicken. PhD thesis, Wageningen University, The Netherlands Understanding the factors influencing sex of egg and sex ratio in laying chicken may lead to finding potential solutions for the problem of killing of day old male chicks, which is the current practice in breeding of laying hens.
    [Show full text]
  • Wormy Cheese, Cloned Pig Meat and Much More for a Kosher Table?
    Wormy Cheese, Cloned Pig Meat and much more for a Kosher table? by Rabbi Dr. Chaim Simons Kiryat Arba, Israel 5779 / 2018 © Copyright Chaim Simons 2018 2 I N D E X * Wormy Cheese with fruit salad for Tu Bishvat 6 * Cloned Pig Meat for Mishloach Manot 12 * Kreplach filled with minced Swans’ meat for Hoshana Raba 16 * Gefilte Turbot for Shabbat dinner 20 * Fried Locusts with yoghurt 25 * Giraffes giblets as hors d'oeuvre for Yom Tov meal 30 * Bees’ Royal Jelly prior to a meal 35 * Nightingale schnitzels for Chol Hamoed 39 * Head of Swordfish for Leil Rosh Hashanah 43 * Rice Cakes for Ashkenazi Jews for Seder night 48 * Fish Blood Borsht for Erev Pesach 52 * Deer’s Kidneys for Melave Malka 56 * Hard boiled Sparrows’ eggs for Erev Tisha b’Av 60 * Pig Bone Gelatin coating on Chanukah Latkes 64 * Caviar from Sturgeon for Seudah Shlishit 68 * Milk dessert following a Meat meal for Shavuot 73 * Zebu meat in Shabbat cholent 77 3 A C K N O W L E D G M E N T S In order to write this book, I had to assemble a vast amount of material. Today, with the Internet, many of the papers and articles which I utilized in writing my book were to be found there. I acknowledge with gratitude the various authors of this material. In particular, I must mention the writings of the following: Rabbi Dr. Ari Zivotofsky, Professor Zohar Amar, Rabbi Dr. J. David Bleich and Dr. Yisrael Meir Levinger. Their material was invaluable for my book.
    [Show full text]
  • Follicle Selection and Growth in the Domestic Hen Ovary
    FOLLICLE SELECTION AND GROWTH IN THE DOMESTIC HEN OVARY A Dissertation Presented to the Faculty of the Graduate School of Cornell University In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy by Claire Stice Stephens August 2015 © 2015 Claire Stice Stephens FOLLICLE SELECTION AND GROWTH IN THE DOMESTIC HEN OVARY Claire Stice Stephens, Ph. D. Cornell University 2015 Ovarian follicle selection and development in domestic hens is highly regulated. A single small follicle (approximately 6-8 mm in diameter) is selected daily and enters the pre-ovulatory hierarchy, continuing to grow until ovulation occurs. We investigated the mechanism(s) involved in follicle selection and subsequent growth before ovulation. In the first study, we found bone morphogenetic protein 15 (BMP15) mRNA primarily in the oocyte, and BMP15 protein was detected in 6 and 8 mm follicles around the time of follicle selection. In addition, BMP15 altered mRNA expression of genes associated with follicle selection. Granulosa cell culture with BMP15 increased mRNA levels of follicle stimulating hormone receptor (FSHR) and decreased both anti-Müllerian hormone (AMH) and occludin (OCLN). OCLN protein, a tight junction protein, also decreased, suggesting that this oocyte factor may play a pivotal role in follicle selection into the pre-ovulatory hierarchy. Liver-derived yolk material enters follicles via capillaries in the theca layer, ultimately reaching oocytes via paracellular spaces between granulosa cells. Yolk is incorporated into the oocyte by receptor-mediated endocytosis. OCLN may regulate access of yolk material to the oocyte surface. We determined expression and regulation of OCLN in small follicles. OCLN mRNA expression decreased with increasing follicle size.
    [Show full text]
  • Oogenesis: Single Cell Development and Differentiation ⁎ Jia L
    Developmental Biology 300 (2006) 385–405 www.elsevier.com/locate/ydbio Oogenesis: Single cell development and differentiation ⁎ Jia L. Song, Julian L. Wong, Gary M. Wessel Department of Molecular and Cellular Biology and Biochemistry, Box G, Brown University, Providence RI 02912, USA Received for publication 10 May 2006; revised 27 July 2006; accepted 28 July 2006 Available online 5 August 2006 Abstract Oocytes express a unique set of genes that are essential for their growth, for meiotic recombination and division, for storage of nutrients, and for fertilization. We have utilized the newly sequenced genome of Strongylocentrotus purpuratus to identify genes that help the oocyte accomplish each of these tasks. This study emphasizes four classes of genes that are specialized for oocyte function: (1) Transcription factors: many of these factors are not significantly expressed in embryos, but are shared by other adult tissues, namely the ovary, testis, and gut. (2) Meiosis: A full set of meiotic genes is present in the sea urchin, including those involved in cohesion, in synaptonemal complex formation, and in meiotic recombination. (3) Yolk uptake and storage: Nutrient storage for use during early embryogenesis is essential to oocyte function in most animals; the sea urchin accomplishes this task by using the major yolk protein and a family of accessory proteins called YP30. Comparison of the YP30 family members across their conserved, tandem fasciclin domains with their intervening introns reveals an incongruence in the evolution of its major clades. (4) Fertilization: This set of genes includes many of the cell surface proteins involved in sperm interaction and in the physical block to polyspermy.
    [Show full text]
  • Cracking The
    Paper Height 308.0mm Height Paper 12.0mm 148.0 x 210.0mm x 148.0 210.0mm x 148.0 6.0mm 6.0mm 6.0mm SOCIETY FOR PROGRAMME AND ABSTRACT BOOK EXPERIMENTAL AN INTEGRATIVE BIOLOGY BIOLOGY OF THE EGG: FROM THE SHELL’S STRUCTURE TO THE PHYSIOLOGY WITHIN Paper Width 450.0mm 3 JULY 2016 BRIGHTON CENTRE, UK 148.0 x 210.0mm CR148.0A CKx 210.0mmING THE EGG 6.0mm 6.0mm SEB Main Office Charles Darwin House 12 Roger Street London, WC1N 2JU T el: +44 (0)20 7685 2600 Fax: + 44 (0)20 7685 2601 [email protected] The Society for Experimental Biology is a registered charity No. 273795 12.0mm SOCIETY FOR EXPERIMENTAL BIOLOGY Paper Height 308.0mm Height Paper 12.0mm 12.0mm 148.0 x 210.0mm x 148.0 210.0mm x 148.0 148.0 x 210.0mm x 148.0 210.0mm x 148.0 6.0mm 6.0mm 6.0mm 6.0mm Paper Width 450.0mm 6.0mm 6.0mm ANIMAL SECTION SATELLITE NOTES 20 ANIMAL SECTION SATELLITE CONTENTS 01 AN INTEGRATIVE BIOLOGY OF THE EGG: FROM THE Paper Width 450.0mm SHELL’S STRUCTURE TO THE PHYSIOLOGY WITHIN 1. DELEGATE INFORMATION 02 2. PROGRAMME 03 3. POSTER SESSION 06 4. ABSTRACTS 07 5. POSTER ABSTRACTS 16 6. AUTHOR INDEX 18 148.0 x 210.0mm 148.0 x 210.0mm 6.0mm 6.0mm 6.0mm 6.0mm ORGANISED BY: DR STEVE PORTUGAL ROYAL HOLLOWAY UNIVERSITY OF LONDON, UNITED KINGDOM PROF MARK HAUBER HUNTER COLLEGE, UNITED STATES 12.0mm MEETING SPONSORED BY: 12.0mm Paper Height 308.0mm Paper Height 308.0mm Height Paper 12.0mm 12.0mm 148.0 x 210.0mm x 148.0 210.0mm x 148.0 148.0 x 210.0mm x 148.0 210.0mm x 148.0 6.0mm 6.0mm 6.0mm 6.0mm Paper Width 450.0mm 6.0mm 6.0mm ANIMAL SECTION SATELLITE DELEGATEAUTHOR
    [Show full text]