Vegetation Lists (Indigenous & Alien)
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Morphological and Molecular Barcode Analysis of the Medicinal Tree Mimusops Coriacea (A.DC.) Miq
Morphological and molecular barcode analysis of the medicinal tree Mimusops coriacea (A.DC.) Miq. collected in Ecuador Katherine Bustamante1, Efrén Santos-Ordóñez2,3, Migdalia Miranda4, Ricardo Pacheco2, Yamilet Gutiérrez5 and Ramón Scull5 1 Facultad de Ciencias Químicas, Ciudadela Universitaria “Salvador Allende,” Universidad de Guayaquil, Guayaquil, Ecuador 2 Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador 3 Facultad de Ciencias de la Vida, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador 4 Facultad de Ciencias Naturales y Matemáticas, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador 5 Instituto de Farmacia y Alimentos, Universidad de La Habana, Ciudad Habana, Cuba ABSTRACT Background: Mimusops coriacea (A.DC.) Miq., (Sapotaceae), originated from Africa, were introduced to coastal areas in Ecuador where it is not extensively used as a traditional medicine to treat various human diseases. Different therapeutically uses of the species include: analgesic, antimicrobial, hypoglycemic, inflammation and pain relieve associated with bone and articulation-related diseases. Furthermore, Mimusops coriacea could be used as anti-oxidant agent. However, botanical, chemical or molecular barcode information related to this much used species is not available from Ecuador. In this study, morphological characterization was performed from leaves, stem and seeds. Furthermore, genetic characterization was performed using molecular barcodes for rbcL, matk, ITS1 and ITS2 using DNA extracted from leaves. Methods: Macro-morphological description was performed on fresh leaves, stem Submitted 25 March 2019 and seeds. For anatomical evaluation, tissues were embedded in paraffin and Accepted 29 August 2019 transversal dissections were done following incubation with sodium hypochlorite Published 11 October 2019 and safranin for coloration and fixated later in glycerinated gelatin. -
Evaluación Genética, Química Y Farmacológica De Mimusops Coriácea TÍTULO Y SUBTÍTULO: (ADC) Miq
UNIVERSIDAD DE GUAYAQUIL FACULTAD DE CIENCIAS QUÍMICAS CARRERA EN QUÍMICA Y FARMACIA TRABAJO DE TITULACIÓN PRESENTADO COMO REQUISITO PREVIO PARA OPTAR AL GRADO DE QUÍMICO Y FARMACÉUTICO. MODALIDAD: INVESTIGACIÓN TEMA: Evaluación genética, química y farmacológica de Mimusops coriácea (ADC) Miq. AUTORES: Loor Moreira Emanuel Sebastián Rendón Plúas Lady Liliana TUTOR: Q.F. Katherine Bustamante Pesantes Mg. GUAYAQUIL - ECUADOR 2019 – 2020 CI i i FACULTAD DE CIENCIAS QUÍMICAS CARRERA QUÍMICA Y FARMACIA UNIDAD DE TITULACIÓN i FICHA DE REGISTRO DE TESIS/TRABAJO DE GRADUACIÓN Evaluación genética, química y farmacológica de Mimusops coriácea TÍTULO Y SUBTÍTULO: (ADC) Miq. AUTOR (ES) Loor Moreira Emanuel Sebastián (Apellidos/Nombres): Rendón Plúas Lady Liliana DOCENTE TUTOR Y TUTOR: Katherine Elizabeth Bustamante Pesantes DOCENTE REVISOR REVISOR: Alexandra Jenny López Barrera (Apellidos/Nombres): INSTITUCIÓN: UNIVERSIDAD DE GUAYAQUIL UNIDAD/FACULTAD: CIENCIAS QUÍMICAS MAESTRÍA/ESPECIALIDAD: GRADO OBTENIDO: QUÍMICO Y FARMACÉUTICO FECHA DE PUBLICACIÓN: Sept/2019 No. DE PÁGINAS: 89 ÁREAS TEMÁTICAS: Ciencia y Tecnología Farmacéutica PALABRAS CLAVES/ KEYWORDS: genotipo, taxonomía, extractos, hojas, corteza, fruto. RESUMEN/ABSTRACT (150-250 palabras): La familia Sapotácea, perteneci ente a las fanerógamas contempla decenas de géneros entre los que destaca Mimusops con varias especies identificadas por su genotipo. Se caracterizaron genéticamente las hojas, y los frutos fueron sometidos a est udio químico y farmacológico junto a la corteza por métodos -
Bark Medicines Used in Traditional Healthcare in Kwazulu-Natal, South Africa: an Inventory
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector South African Journal of Botany 2003, 69(3): 301–363 Copyright © NISC Pty Ltd Printed in South Africa — All rights reserved SOUTH AFRICAN JOURNAL OF BOTANY ISSN 0254–6299 Bark medicines used in traditional healthcare in KwaZulu-Natal, South Africa: An inventory OM Grace1, HDV Prendergast2, AK Jäger3 and J van Staden1* 1 Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa 2 Centre for Economic Botany, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AE, United Kingdom 3 Department of Medicinal Chemistry, Royal Danish School of Pharmacy, 2 Universitetsparken, 2100 Copenhagen 0, Denmark * Corresponding author, e-mail: [email protected] Received 13 June 2002, accepted in revised form 14 March 2003 Bark is an important source of medicine in South Overlapping vernacular names recorded in the literature African traditional healthcare but is poorly documented. indicated that it may be unreliable in local plant identifi- From thorough surveys of the popular ethnobotanical cations. Most (43%) bark medicines were documented literature, and other less widely available sources, 174 for the treatment of internal ailments. Sixteen percent of species (spanning 108 genera and 50 families) used for species were classed in threatened conservation cate- their bark in KwaZulu-Natal, were inventoried. gories, but conservation and management data were Vernacular names, morphological and phytochemical limited or absent from a further 62%. There is a need for properties, usage and conservation data were captured research and specialist publications to address the in a database that aimed to synthesise published infor- gaps in existing knowledge of medicinal bark species mation of such species. -
Accounting for Variation of Substitution Rates Through Time in Bayesian Phylogeny Reconstruction of Sapotoideae (Sapotaceae)
Molecular Phylogenetics and Evolution 39 (2006) 706–721 www.elsevier.com/locate/ympev Accounting for variation of substitution rates through time in Bayesian phylogeny reconstruction of Sapotoideae (Sapotaceae) Jenny E.E. Smedmark ¤, Ulf Swenson, Arne A. Anderberg Department of Phanerogamic Botany, Swedish Museum of Natural History, P.O. Box 50007, SE-104 05 Stockholm, Sweden Received 9 September 2005; revised 4 January 2006; accepted 12 January 2006 Available online 21 February 2006 Abstract We used Bayesian phylogenetic analysis of 5 kb of chloroplast DNA data from 68 Sapotaceae species to clarify phylogenetic relation- ships within Sapotoideae, one of the two major clades within Sapotaceae. Variation in substitution rates through time was shown to be a very important aspect of molecular evolution for this data set. Relative rates tests indicated that changes in overall rate have taken place in several lineages during the history of the group and Bayes factors strongly supported a covarion model, which allows the rate of a site to vary over time, over commonly used models that only allow rates to vary across sites. Rate variation over time was actually found to be a more important model component than rate variation across sites. The covarion model was originally developed for coding gene sequences and has so far only been tested for this type of data. The fact that it performed so well with the present data set, consisting mainly of data from noncoding spacer regions, suggests that it deserves a wider consideration in model based phylogenetic inference. Repeatability of phylogenetic results was very diYcult to obtain with the more parameter rich models, and analyses with identical settings often supported diVerent topologies. -
(Mimusops Caffra) and Transvaal Red
ELEMENTAL COMPOSITION AND NUTRITIONAL VALUE OF THE EDIBLE FRUITS OF COASTAL RED MILKWOOD (MIMUSOPS CAFFRA) AND TRANSVAAL RED MILKWOOD (MIMUSOPS ZEYHERI) AND THE IMPACT OF SOIL QUALITY by Sihle Vitalis Mngadi Submitted in fulfilment of the academic requirement for the degree of Master of Science in the School of Chemistry and Physics, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Durban, South Africa 2017 Elemental Composition and Nutritional value of the edible fruits of Coastal red milkwood (Mimusops caffra) and Transvaal red milkwood (Mimusops zeyheri) and the impact of soil quality SIHLE VITALIS MNGADI 2017 A thesis submitted to the School of Chemistry and Physics, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville, for the Degree of Master of Science. This thesis has been prepared according to Format 4 as outlined in the guidelines from the College of Agriculture, Engineering and Science which states: This is a thesis in which chapters are written as a set of discrete research papers, with an overall introduction and final discussion where one (or all) of the chapters have already been published. Typically, these chapters will have been published in internationally recognized, peer- reviewed journals. As the candidate’s supervisor(s), I have approved this thesis for submission. Supervisor: Name: Prof SB Jonnalagadda Signature Date: Co-Supervisor: Name: Dr Roshila Moodley Signature Date: 11/07/2017 II ABSTRACT Mimusops caffra and Mimusops zeyheri, both of the plant family Sapotaceae are indigenous plant species that grow widely in most parts of South Africa and the edible fruits of these species are picked and eaten raw in rural communities across South Africa. -
NUMBERED TREE SPECIES LIST in SOUTH AFRICA CYATHEACEAE 1 Cyathea Dregei 2 Cyathea Capensis Var. Capensis ZAMIACEAE 3 Encephalart
NUMBERED TREE SPECIES LIST IN SOUTH AFRICA 23 Hyphaene coriacea CYATHEACEAE 24 Hyphaene petersiana 1 Cyathea dregei 25 Borassus aethiopum 2 Cyathea capensis var. capensis 26 Raphia australis 27 Jubaeopsis caffra ZAMIACEAE 3 Encephalartos altensteinii ASPHODELACEAE 3.1 Encephalartos eugene-maraisii 28 Aloe barberae 3.2 Encephalartos arenarius 28.1 Aloe arborescens 3.3 Encephalartos brevifoliolatus 28.2 Aloe africana 3.4 Encephalartos ferox 28.3 Aloe alooides 4 Encephalartos friderici-guilielmi 28.4 Aloe angelica 5 Encephalartos ghellinckii 28.5 Aloe candelabrum 5.1 Encephalartos inopinus 28.6 Aloe castanea 5.2 Encephalartos lanatus 28.7 Aloe comosa 6 Encephalartos laevifolius 28.8 Aloe excelsa var. excelsa 7 Encephalartos latifrons 29 Aloe dichotoma 8 Encephalartos senticosus 29.1 Aloe dolomitica 8.1 Encephalartos lehmannii 29.2 Aloe ferox 9 Encephalartos longifolius 29.3 Aloe khamiesensis 10 Encephalartos natalensis 29.4 Aloe littoralis 11 Encephalartos paucidentatus 29.5 Aloe marlothii subsp. marlothii 12 Encephalartos princeps 29.6 Aloe plicatilis 12.5 Encephalartos relictus 29.7 Aloe marlothii subsp. orientalis 13 Encephalartos transvenosus 30 Aloe pillansii 14 Encephalartos woodii 30.1 Aloe pluridens 14.1 Encephalartos heenanii 30.2 Aloe ramosissima 14.2 Encephalartos dyerianus 30.3 Aloe rupestris 14.3 Encephalartos middelburgensis 30.4 Aloe spicata 14.4 Encephalartos dolomiticus 30.5 Aloe speciosa 14.5 Encephalartos aemulans 30.6 Aloe spectabilis 14.6 Encephalartos hirsutus 30.7 Aloe thraskii 14.7 Encephalartos msinganus 14.8 Encephalartos -
Regeneration of a Natal Coastal Dune Forest After Fire
Regeneration of a Natal coastal dune forest after frre N.W. Pammenter, M. Berjak and I.A.W. Macdonald Department of Biological Sciences and Department of Computer Science, University of Natal, Durban and Natal Parks Board, Pietermaritzburg The composition of a coastal dune forest in the Mlalazi Introduction Nature Reserve, Natal was studied three years after a fire Reports on the effects of fire on forest communities in Africa and compared with adjacent unburnt forest. The two have been confined largely to accounts of how frequent communities had similar plant densities but there was a marked decrease in biomass and diversity in the burnt burning, often in combination with shifting cultivation or community. Measured as density, basal area or canopy biotic influences, has led to the replacement of forest volume, Trema orienta/is contributed over 70% of the communities by derived savannas and grasslands (e.g. Swyn regenerating burnt forest. Saplings of some forest trees nerton 1918; Aubreville 1947; Beuchner & Dawkins 1961; were. present but those of Mimusops caffra, the dominant West 1971). Very little attention has been paid to the effects tree of the unburnt community, were absent from the burnt of 'natural' forest fire regimes in Africa although it is likely area. S. Afr. J. Bot. 1985, 51: 453 - 459 that forest communities in the region have always experienced Die samestelling van 'n kusduinwoud te Mlalazi· periodic fires, albeit predominantly on the margins. The natuurreservaat, Natal is drie jaar na 'n brand bestudeer en incidence of forest fires would probably have been highest met aangrensende ongebrande woud vergelyk. -
A Checklist of Lesotho Grasses
Guidelines for Using the Checklist The genera and species are arranged in alphabetical order. Accepted genus and species names are in bold print, for example, Agrostis barbuligera. Synonyms are in italics, for example, Agrostis natalensis. Not all synonyms for a species are listed. Naturalised taxa are preceded by an asterisk, for example, Pennisetum *clandestinum. These are species that were intro- duced from outside Lesotho but now occur in the wild as part of the natural flora. Single letters after the species names, on the right-hand side of the column, indicate the distribution of species within Lesotho as reflected by the ROML and MASE collections. This indicates that a species has definitely been recorded in Lesotho. L—Lowlands F—Foothills M—Mountains S—Senqu Valley Double letters after species names, on the right-hand side of the column, indicate the distribution of species along the border with South Africa as reflected in the literature. This indicates that a species could occur in Lesotho, but has not yet been recorded. KN—KwaZulu-Natal FS—Free State EC—Eastern Cape Literature references are abbreviated as follows: G—Gibbs Russell et al. (1990) J—Jacot Guillarmod (1971) SCH—Schmitz (1984) V—Van Oudtshoorn (1999) For example, G:103 refers to page 103 in the Gibbs Russell et al. (1990) publication, Grasses of southern Africa. The seven-digit number to the right of the genus names is the numbering system followed at Kew Herbarium (K) and used in Arnold & De Wet (1993) and Leistner (2000). N M F L M Free State S Kwa-Zulu Natal Key L Lowlands Zone Maize (Mabalane) F Foothills Zone Sorghum M Mountain Zone Wheat (Maloti) S Senqu Valley Zone Peas Cattle Beans Scale 1 : 1 500 000 Sheep and goats 20 40 60 km Eastern Cape Zones of Lesotho based on agricultural practices. -
A Classification of the Chloridoideae (Poaceae)
Molecular Phylogenetics and Evolution 55 (2010) 580–598 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev A classification of the Chloridoideae (Poaceae) based on multi-gene phylogenetic trees Paul M. Peterson a,*, Konstantin Romaschenko a,b, Gabriel Johnson c a Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC 20013, USA b Botanic Institute of Barcelona (CSICÀICUB), Pg. del Migdia, s.n., 08038 Barcelona, Spain c Department of Botany and Laboratories of Analytical Biology, Smithsonian Institution, Suitland, MD 20746, USA article info abstract Article history: We conducted a molecular phylogenetic study of the subfamily Chloridoideae using six plastid DNA Received 29 July 2009 sequences (ndhA intron, ndhF, rps16-trnK, rps16 intron, rps3, and rpl32-trnL) and a single nuclear ITS Revised 31 December 2009 DNA sequence. Our large original data set includes 246 species (17.3%) representing 95 genera (66%) Accepted 19 January 2010 of the grasses currently placed in the Chloridoideae. The maximum likelihood and Bayesian analysis of Available online 22 January 2010 DNA sequences provides strong support for the monophyly of the Chloridoideae; followed by, in order of divergence: a Triraphideae clade with Neyraudia sister to Triraphis; an Eragrostideae clade with the Keywords: Cotteinae (includes Cottea and Enneapogon) sister to the Uniolinae (includes Entoplocamia, Tetrachne, Biogeography and Uniola), and a terminal Eragrostidinae clade of Ectrosia, Harpachne, and Psammagrostis embedded Classification Chloridoideae in a polyphyletic Eragrostis; a Zoysieae clade with Urochondra sister to a Zoysiinae (Zoysia) clade, and a Grasses terminal Sporobolinae clade that includes Spartina, Calamovilfa, Pogoneura, and Crypsis embedded in a Molecular systematics polyphyletic Sporobolus; and a very large terminal Cynodonteae clade that includes 13 monophyletic sub- Phylogenetic trees tribes. -
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92 Bothalia 24,1 (1994) recognised as one of the principal methods for the forma REFERENCES tion of new species among higher plants’, but that the CRON, G.V. 1991. A sy stematic study o f certain species of Cineraria L species originating from this process are usually ‘very (Asteraceae). M.Sc. thesis, University of the Witwatersrand, Jo similar to their diploid ancestors in external morphology hannesburg. and ecological preferences’. C. albicans is a highly vari DARLINGTON, C.D. & LA COUR, F.L. 1976. The handling of chromo able species (Hilliard 1977; Cron 1991) and may well be somes. George Allen & Unwin, London. a species complex or in the process of speciating (Cron DUBININ, N.P. 1940. Darwinism and the genetics of populations. 1991). Characters such as the size and persistence of the Uspekhi Sovremennoi Biologii 13: 276—305. petiolar auricles, the glabrescence of the involucral bracts DYER, R.A. 1963. The use of lactopropionic orcein in rapid squash and the indumentum of the cypselae show great variation. methods for chromosome preparations. Stain Technology 38: 85- 90. (Note: the 'species A' of Hilliard (1977) is here considered GOLDBLATT, P. 1981. Index to plant chaimosome numbers 1975-1978. to be part of C. albicans.) Apparent isolation of popula Monographs in Systematic Botany 5. Braun-Brumfield, Ann tions of C. albicans in the gorges comprising valley Arbor, Michigan. bushveld in the Natal midlands and coastal areas, and in GRANT, V.E. 1971. Plant speciation. Columbia University Press, New Transkei has evidently resulted in variability becoming York and London. fixed in certain areas (Dubinin 1940). -
A Molecular Phylogeny and Classification of the Cynodonteae
TAXON 65 (6) • December 2016: 1263–1287 Peterson & al. • Phylogeny and classification of the Cynodonteae A molecular phylogeny and classification of the Cynodonteae (Poaceae: Chloridoideae) with four new genera: Orthacanthus, Triplasiella, Tripogonella, and Zaqiqah; three new subtribes: Dactylocteniinae, Orininae, and Zaqiqahinae; and a subgeneric classification of Distichlis Paul M. Peterson,1 Konstantin Romaschenko,1,2 & Yolanda Herrera Arrieta3 1 Smithsonian Institution, Department of Botany, National Museum of Natural History, Washington, D.C. 20013-7012, U.S.A. 2 M.G. Kholodny Institute of Botany, National Academy of Sciences, Kiev 01601, Ukraine 3 Instituto Politécnico Nacional, CIIDIR Unidad Durango-COFAA, Durango, C.P. 34220, Mexico Author for correspondence: Paul M. Peterson, [email protected] ORCID PMP, http://orcid.org/0000-0001-9405-5528; KR, http://orcid.org/0000-0002-7248-4193 DOI https://doi.org/10.12705/656.4 Abstract Morphologically, the tribe Cynodonteae is a diverse group of grasses containing about 839 species in 96 genera and 18 subtribes, found primarily in Africa, Asia, Australia, and the Americas. Because the classification of these genera and spe cies has been poorly understood, we conducted a phylogenetic analysis on 213 species (389 samples) in the Cynodonteae using sequence data from seven plastid regions (rps16-trnK spacer, rps16 intron, rpoC2, rpl32-trnL spacer, ndhF, ndhA intron, ccsA) and the nuclear ribosomal internal transcribed spacer regions (ITS 1 & 2) to infer evolutionary relationships and refine the -
Population Assessments of Priority Plant Species Used by Local Communities in and Around Three Wild Coast Reserves, Eastern Cape, South Africa
Population assessments of priority plant species used by local communities in and around three Wild Coast reserves, Eastern Cape, South Africa By JOCLYN J. FEARON Submitted in fulfillment of the requirements for the degree of Masters of Science Department of Environmental Science, Rhodes University Grahamstown 2010 Abstract The project was initiated by Eastern Cape Parks (ECP) as a request for the construction of inventories of priority species and their population levels inside three nature reserves on the Eastern Cape Wild Coast, South Africa, and to develop a strategic management plan to manage these natural resources in each reserve. Thirty key species were identified by local communities in and around Dwesa-Cwebe, Silaka and Mkambati Nature Reserves through community workshops. For forested areas belt transects of 100 m x 6 m where used. The basal circumference of key tree species within the belt transect was measured as well as the height of saplings (height < 150 m). Tree species were categorized based on densities, size class distribution (SCD) curves and values, and spatial grain. For grassland areas straight transects of 200 m long were used, along which ten 3 m x 3 m quadrates were placed at 20 m intervals. Within each grassland transect the height of herbs or tuft diameter of grasses was recorded and percentage cover estimated. Grassland species were categorized based on density, SCD curves and percentage cover. All species were placed into harvesting categories based on analysed ecological data that was collected in the field. Category 1 species were very rare or not found in the reserve and it was recommended that species be conserved and monitored.