Introduction to Honey Bees and Beekeeping
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Honey Bee Immunity — Pesticides — Pests and Diseases
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Distance Master of Science in Entomology Projects Entomology, Department of 2017 A GUIDEBOOK ON HONEY BEE HEALTH: Honey Bee Immunity — Pesticides — Pests and Diseases Joey Caputo Follow this and additional works at: https://digitalcommons.unl.edu/entodistmasters Part of the Entomology Commons This Article is brought to you for free and open access by the Entomology, Department of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Distance Master of Science in Entomology Projects by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Photo by David Cappaert, Bugwood.org 1 A GUIDEBOOK ON HONEY BEE HEALTH Honey Bee Immunity — Pesticides— Pests and Diseases By Joey Caputo A graduate degree project submitted as partial fulfillment of the Option III requirements for the de- gree of Masters of Science in Entomology at the graduate school of the University of Nebraska- Lincoln, 2017. Last updated April 2017 — Version 1.2 i Contents Introduction 1 Honey Bee Immune System 2 Mechanical and Biochemical Immunity 2 Innate and Cell-Mediated Immunity 2 Humoral Immunity 2 Social Immunity 3 Detoxification Complexes 5 Problems in Beekeeping 5 Colony Collapse Disorder (CCD) 5 Bacterial, Fungal and Microsporidian Diseases 6 American foulbrood 6 European foulbrood 7 Nosemosis 8 Chalkbrood 10 Crithidia 10 Stonebrood 11 Varroa Mite and Viruses 11 Varroa Biology and Life Cycle 12 Varroa Mite Damage and Parasitic Mite -
Minimizing Honey Bee Exposure to Pesticides1 J
ENY-162 Minimizing Honey Bee Exposure to Pesticides1 J. D. Ellis, J. Klopchin, E. Buss, L. Diepenbrock, F. M. Fishel, W. H. Kern, C. Mannion, E. McAvoy, L. S. Osborne, M. Rogers, M. Sanford, H. Smith, B. S. Stanford, P. Stansly, L. Stelinski, S. Webb, and A. Vu2 Introduction state, and international partners to identify ways to reduce pesticide exposure to beneficial pollinators, while including Growers and pesticide applicators have a number of options appropriate label restrictions to safeguard pollinators, the when faced with a pest problem: do nothing, or apply environment, and humans. More information can be found some type of cultural, chemical, biological, or physical here: epa.gov/pollinator-protection. The bottom line is that method to mitigate the damage. The action to be taken the label is the law—it must be followed. should be chosen after weighing the risks and benefits of all available options. There are many situations where pest control is necessary and chemical controls must be Pollinator Importance used. Certain chemistries of insecticides, fungicides, and The western honey bee (Apis mellifera, Figure 1) is conceiv- herbicides are known to have negative and long-term ably the most important pollinator in Florida and American impacts on bees, other pollinators, and other beneficial agricultural landscapes (Calderone 2012). Over 50 major arthropods. Fortunately, there are pesticides that have crops in the United States and at least 13 in Florida either minimal impacts on pollinators and beneficial organisms. depend on honey bees for pollination or produce more The pollinator-protection language that is required to be yield when honey bees are plentiful (Delaplane and on US pesticide labels outlines how best to minimize these Mayer 2000). -
Save the Bees Save the Bees
Unit for week 5 save the bees Save the bees Stresses on the Honey bee Several factors may create stress in the hive, which can cause a decrease in population. Below are some of those possible contributors. All of these effects on the colony can be observed, some more easily than others, in the Observation Hive. VARROA MITES: The Varroa mite is a parasitic, invasive species that was introduced to the United States in the 1980’s . It BEYOND THE originated in Asia and the western honey bee has no resistance. The mated adult female Varroa mites enter the brood cells right before HIVE the bees cap the pupae and feed on the growing bee. The bee will hatch with deformities such as misshapen wings that result in an inability to fly. SMALL HIVE BEETLES: Hive beetles are pests to honey bees. Ask the Audience They entered the United States in the late 90’s. Most strong hives will not be severely affected by the beetle; however, if the hive • Do you know what it feels like beetle becomes too overbearing, the colony will desert the hive. The to be stressed? beetle tunnels in the comb and creates destruction in the storage of honey and pollen. Ways to identify a beetle problem is a smell of • Do you have any pests in your fermented honey, a slimy covering of the comb, and the presence life? of beetle maggots. • Do you have a vegetable DISEASE: although bees keep their hive very clean and try to garden or any flowers in your maintain sanitation as best as possible, there are many pathogens, yard? disease causing microorganisms, which can infect the bees. -
Factors Affecting Global Bee Health
CropLife International A.I.S.B.L. Avenue Louise 326, box 35 - B-1050 - Brussels – Belgium TEL +32 2 542 04 10 FAX +32 2 542 04 19 www.croplife.org Factors Affecting Global Bee Health Honey Bee Health and Population Losses in Managed Bee Colonies Prepared by Diane Castle for CropLife International May 2013 Table of Contents Summary ............................................................................................................................................. 1 1.0 Introduction ................................................................................................................................ 1 2.0 Pest and Diseases Affecting Honey Bees ..................................................................... 2 2.1 Parasitic Mites ................................................................................................................................ 3 2.1.1 Varroa Destructor .................................................................................................................... 3 2.1.2 The Role of V Destructor in Colony Losses ............................................................................ 3 2.1.3 Control of Varroa Mites ........................................................................................................... 4 2.2 Viral infections ................................................................................................................................ 5 2.2.1 Key Viral Infections Implicated in Colony Loss ...................................................................... -
Honey Bees: a Guide for Veterinarians
the veterinarian’s role in honey bee health HONEY BEES: A GUIDE FOR VETERINARIANS 01.01.17 TABLE OF CONTENTS Introduction Honey bees and veterinarians Honey bee basics and terminology Beekeeping equipment and terminology Honey bee hive inspection Signs of honey bee health Honey bee diseases Bacterial diseases American foulbrood (AFB) European foulbrood (EFB) Diseases that look like AFB and EFB Idiopathic Brood Disease (IBD) Parasitic Mite Syndrome (PMS) Viruses Paralytic viruses Sacbrood Microsporidial diseases Nosema Fungal diseases Chalkbrood Parasitic diseases Parasitic Mite Syndrome (PMS) Tracheal mites Small hive beetles Tropilaelaps species Other disease conditions Malnutrition Pesticide toxicity Diploid drone syndrome Overly hygienic hive Drone-laying queen Laying Worker Colony Collapse Disorder Submission of samples for laboratory testing Honeybee Flowchart (used with permission from One Health Veterinary Consulting, Inc.) Additional Resources Acknowledgements © American Veterinary Medical Association 2017. This information has not been approved by the AVMA Board of Directors or the House of Delegates, and it is not to be construed as AVMA policy nor as a definitive statement on the subject, but rather to serve as a resource providing practical information for veterinarians. INTRODUCTION Honey bees weren’t on veterinarians’ radars until the U.S. Food and Drug Administration issued a final Veterinary Feed Directive (VFD) rule, effective January 1, 2017, that classifies honey bees as livestock and places them under the provisions of the VFD. As a result of that rule and changes in the FDA’s policy on medically important antimicrobials, honey bees now fall into the veterinarians’ purview, and veterinarians need to know about their care. -
Life Cycles: Egg to Bee Free
FREE LIFE CYCLES: EGG TO BEE PDF Camilla de La Bedoyere | 24 pages | 01 Mar 2012 | QED PUBLISHING | 9781848355859 | English | London, United Kingdom Tracking the Life Cycle of a Honey Bee - dummies As we remove the frames, glance over the thousands of busy bees, check for brood, check for capped honey, maybe spot the queen… then the frames go back in their slots and the hive is sealed up again. But in the hours spent away from our hives, thousands of tiny miracles are happening everyday. Within the hexagonal wax cells little lives are hatching out and joining the hive family. The whole process from egg to adult worker bee takes around 18 days. During the laying season late spring to summer the Queen bee is capable of laying over eggs per day. Her worker bees help direct her to the best prepared comb and she lays a single egg in each hexagon shaped cell. The size of the cell prepared determines the type of egg she lays. If the worker bees have prepared a worker size cell, she Life Cycles: Egg to Bee lay a fertilized egg. This egg will produce a female worker bee. If the worker bees have prepared a slightly larger cell, the queen will recognize this as a drone cell and lay an unfertilized egg. This will produce a male drone bee. It is the workers and not the queen that determine the ratio of workers to drones within the hive. In three days the egg hatches and a larva emerges. It looks very similar to a small maggot. -
Effect of Wood Preservative Treatment of Beehives on Honey Bees Ad Hive Products
1176 J. Agric. Food Chem. 1984. 32, 1176-1180 Effect of Wood Preservative Treatment of Beehives on Honey Bees and Hive Products Martins A. Kalnins* and Benjamin F. Detroy Effects of wood preservatives on the microenvironment in treated beehives were assessed by measuring performance of honey bee (Apis mellifera L.) colonies and levels of preservative residues in bees, honey, and beeswax. Five hives were used for each preservative treatment: copper naphthenate, copper 8-quinolinolate, pentachlorophenol (PCP), chromated copper arsenate (CCA), acid copper chromate (ACC), tributyltin oxide (TBTO), Forest Products Laboratory water repellent, and no treatment (control). Honey, beeswax, and honey bees were sampled periodically during two successive summers. Elevated levels of PCP and tin were found in bees and beeswax from hives treated with those preservatives. A detectable rise in copper content of honey was found in samples from hives treated with copper na- phthenate. CCA treatment resulted in an increased arsenic content of bees from those hives. CCA, TBTO, and PCP treatments of beehives were associated with winter losses of colonies. Each year in the United States, about 4.1 million colo- honey. Harmful effect of arsenic compounds on bees was nies of honey bees (Apis mellifera L.) produce approxi- linked to orchard sprays and emissions from smelters in mately 225 million pounds of honey and 3.4 million pounds a Utah study by Knowlton et al. (1947). An average of of beeswax. This represents an annual income of about approximately 0.1 µg of arsenic trioxide/dead bee was $140 million; the agricultural economy receives an addi- reported. -
Bee Varroa Parasitosis Control
15 November 2010 EMA/CVMP/EWP/324712/2010 Committee for Medicinal Products for Veterinary Use (CVMP) Overview of comments received on 'Guideline on veterinary medicinal products controlling varroa destructor parasitosis in bees' (EMEA/CVMP/EWP/459883/2008-CONSULTATION) Interested parties (organisations or individuals) that commented on the draft document as released for consultation. Stakeholder no. Name of organisation or individual 1 Danish Beekeepers Association 2 7 Westferry Circus ● Canary Wharf ● London E14 4HB ● United Kingdom Telephone +44 (0)20 7418 8400 Facsimile +44 (0)20 7418 8416 E-mail [email protected] Website www.ema.europa.eu An agency of the European Union © European Medicines Agency, 2014. Reproduction is authorised provided the source is acknowledged. 1. General comments – overview Stakeholder General comment Outcome No. 1 The vast majority of beekeepers provide beeswax-foundation Indeed, some acaricides can lead to residues in honey. Honey always contains for their bees based on recycled beeswax from old comb. wax. Both water soluble and organic solvent soluble substances may end-up in honey. Some hydrophobic veterinary medicinal products or their metabolites may contaminate the beeswax and lead to In relation to the potential contamination of honey with residues transferred increasing levels by repeated recycles of beeswax from from wax it should be noted that the MRL set for honey does not distinguish treated colonies. The accumulation is dependent on the between residues incurred as a result of treatment and residues incurred as a stability of the compounds to the heat-treatment in the wax- result of transfer from wax. In addition, it is acknowledged that wax particles melting process. -
Bee Health: the Role of Pesticides
Bee Health: The Role of Pesticides Renée Johnson Specialist in Agricultural Policy M. Lynne Corn Specialist in Natural Resources Policy February 17, 2015 Congressional Research Service 7-5700 www.crs.gov R43900 Bee Health: The Role of Pesticides Summary Over the past few decades there has been heightened concern about the plight of honey bees as well as other bee species. Given the importance of honey bees and other bee species to food production, many have expressed concern about whether a “pollinator crisis” has been occurring in recent decades. Although honey bee colony losses due to bee pests, parasites, pathogens, and disease are not uncommon, there is the perception that bee health has been declining more rapidly than in prior years, both in the United States and globally. This situation gained increased attention in 2006 as some commercial beekeepers began reporting sharp declines in their honey bee colonies. Because of the severity and unusual circumstances of these colony declines, scientists named this phenomenon colony collapse disorder (CCD). Since then, honey bee colonies have continued to dwindle each year, for reasons not solely attributable to CCD. The U.S. Department of Agriculture (USDA) reports that CCD may not be the only or even the major cause of bee colony losses in recent years. In the United States, USDA estimates of overwinter colony losses from all causes have averaged nearly 30% annually since 2006. The precise reasons for honey bee losses are not yet known. USDA and most scientists working on the subject seem to agree that no research conclusively points to one single cause for the large number of honey bee deaths. -
Preventing Pests and Pathogens in Honey Bee Colonies
BestBest ManagementManagement PracticesPractices toto PreventPrevent thethe SpreadSpread ofof PestsPests andand PathogensPathogens inin HoneybeeHoneybee ColoniesColonies Photos by Rob Snyder (used with permission) Many pest and disease problems in managed honeybee hives can be avoided by practic- ing good sanitation and cultural controls. Prevention is the first and best line of defense against organisms that can harm your colonies. Sanitation Tools should be sterilized with flame and scrubbed with isopropyl alcohol after working in or inspecting a hive. Avoid using other beekeeper’s tools that have not been properly cleaned. Clothing and gloves that are exposed to a hive where disease is suspected needs to be scrubbed and disinfected with 10% bleach solution or disposed. If not using gloves, rinse hands with rubbing alcohol then scrub with soap and water after working in a hive that appears to have been infected with disease. When disease is suspected, practice the previously mentioned steps between working hive to hive in the same beeyard. Cultural Controls When purchasing a bee colony, find out if the seller has been treating with antibiotics for patho- gens. Treated colonies could already be infected with disease, even in the absence of symptoms. Never switch frames from a box that is suspected to have pests and pathogens to a box without such problems. Do not purchase or accept used frames, boxes, or other beekeeping equipment that have not been inspected and certified by your county’s bee inspector. Boxes infected with American Foulbrood should be marked with the letters “AFB” followed by the year to prevent unintentional contamination. Equipment that has been infected with American Foulbrood must be treated or burned and buried. -
Wax Worms (Galleria Mellonella) As Potential Bioremediators for Plastic Pollution Student Researcher: Alexandria Elliott Faculty Mentor: Danielle Garneau, Ph.D
Wax Worms (Galleria mellonella) as Potential Bioremediators for Plastic Pollution Student Researcher: Alexandria Elliott Faculty Mentor: Danielle Garneau, Ph.D. Center for Earth and Environmental Science SUNY Plattsburgh, Plattsburgh, NY 12901 Plastic Pollution Life History Stages Results Discussion • 30 million tons of plastic Combination of holes in plastic and • Egg stage: average length (0.478mm) • waste is generated annually and width (0.394mm) and persists 3-10 nylon in frass suggests worms are in the USA (Coalition 2018). days (Kwadha et al. 2017)(Fig. 3). digesting plastic (Figs. 6,7). • Larval stage: max length (30mm), white Of the plastic pilot trials which • 50% landfill, < 10% cream in color, possess 3 apical teeth, exhibited signs of feeding, two were HDPE (Fig. 6). recycled (PlasticsEurope, and persists 22-69 days. A Plastics The Facts 2013) • Pre-pupal/Pupal stage: length (12- • Bombelli et al. (2017) and Yang et al. 20mm) and persists 3-12 and 8-10 days, (2014) found wax worms were capable respectively. All extremities are glued to of PE consumption. • 10% of world’s plastic waste body with molting substance. Common bond (CH -CH ) in PE is 2 2 ends up in ocean 70% • Moth stage: sexual dimorphism is same as that in beeswax (Bombelli et sinks 30% floats in Fig. 1. Plastic Use distinct. Moths max length (20mm) and Fig. 5. Change in worm weight as a function of plastic pilot trial. al. 2017). Fig. 9. PE degradation as currents (Gyres, Fig. 2). (Plastics Europe). persists on average 6-14 days (males) B • Greater negative change in worm weight (g)/day for all FT-IR shows degradation of PE (i.e., evidenced by FT-IR PE and 23 days (females). -
Colony Collapse Disorder in Relation to Human-Produced Toxins: What's
Colony Collapse Disorder in relation to human-produced toxins: What’s the buzz all about? Available at: http://www.sawyoo.com/postpic/2013/09/honey-bee-hives_77452.jpg Last accessed: 17/04/2017 Abstract: p2 Introduction: p3 Insecticides: p5 Herbicides & fungicides: p7 Miticides & other preventative measures: p9 “Inactive” ingredients: p10 Synergies between pesticides: p11 Conclusions: p12 Discussion: p12 References: p14 1 Abstract In recent years, the global population of pollinating animals has been in decline. The honey bee in particular is one of the most important and well known pollinators and is no exception.The Western honey bee Apis mellifera, the most globally spread honey bee species suffers from one problem in particular. Colony Collapse Disorder (CCD), which causes the almost all the worker bees to abandon a seemingly healthy and food rich hive during the winter. One possible explanation for this disorder is that it is because of the several human produced toxins, such as insecticides, herbicides, fungicides and miticides. So the main question is: Are human-produced toxins the primary cause of CCD? It seems that insecticides and, in particular, neonicotinoid insecticides caused increased mortality and even recreated CCD-like symptoms by feeding the bees with neonicotinoids. Herbicides seem relatively safe for bees, though they do indirectly reduce the pollen diversity, which can cause the hive to suffer from malnutrition. Fungicides are more dangerous, causing several sublethal effects, including a reduced immune response and changing the bacterial gut community. The levels of one fungicide in particular, chlorothalonil, tends to be high in hives. Miticides levels tend to be high in treated hives and can cause result in bees having a reduced lifespan.