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Propagation Protocols for Indigenous/Endangered and Exotic Forest Tree Species and Some Fruit Trees

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Propagation Protocols for Indigenous/Endangered and Exotic Forest Tree Species and Some Fruit Trees Department of Environment and Natural Resources ECOSYSTEMS RESEARCH AND DEVELOPMENT BUREAU College, Laguna 4031 Information Bulletin # 1 June 2011 Propagation Protocols for Indigenous/Endangered and Exotic Forest Tree Species and Some Fruit Trees 1 National Greening Program Production of Quality Planting Materials National Coordinator - Director Marcial C. Amaro, Jr. Assistant National Coordinator - Rafael T. Cadiz Technical Working Group : Core Member - Marilyn Q. Landicho Group Leader - Maria DP. Dayan Members - Cristina A. Roxas Paulino A. Umali Jose Allan Castillo Aerosmith M. Masa (Project Staff) Information Bulletin # 1: Propagation Protocols for Indigenous And Exotic Forest Tree Species and Some Fruit Trees June 2011 2 PROPAGATION PROTOCOLS FOR INDIGENOUS AND EXOTIC FOREST TREE SPECIES AND SOME FRUIT TREES Introduction This information bulletin is a compilation of forest tree seed and clonal propagation protocols developed by ERDB from 1995 to 2007. The said protocols were lifted from the Seed Technology Manual of Some Reforestation Species, Forest Tree Seeds: A Phenological Guidebook (1995); DENR Recommends for Indigenous Species in Laguna #15a (2006) and #15b (2007); and Manual on Mass Propagation of Dipterocarps Using Non-mist Technique. The basic information contained in this bulletin on forest tree seed technology and seedling production includes common and scientific names, seed count/kg, pretreatments needed to enhance seed germination and rooting of cuttings, sowing medium to guide the end- users in the propagation of the species needed in the National Greening Program for the production of high quality planting materials (NGP-PQPM) of the Department of Environment and Natural Resources. The mass propagation of some dipterocarp (indigenous) species includes the use of non-mist technology as an effective way for rooting cuttings. The available propagation protocols for few fruit trees developed by the Horticulture Department of UPLB and published by PCARRD are also included to support the 1. Tindalo ± Afzelia rhomboidea (Blanco) agroforestryVidal activity of the various clusters and regions. Place and date of collection: A. INDIGENOUSLobo, Batangas FOREST TREE ± Oct- DecSEEDS Sorsogon ± Oct Albay ± Feb Leyte and Samar ± Dec Seed count: 230/kg 3 2. Akle ± Albizia acle (Blanco) Merr. x Place and date of collection: ¾ Sariaya, Quezon ± May-June ¾ Camp 7, Minglanilla, Cebu ± Oct x Seed count: 597/kg x Seed type: Orthodox x Pretreatment: Nicking or cutting of the seed coat and soaking in tap water or in 0.02% fungicide solution overnight x Sowing medium: 1:1:1 garden soil, dried humus, and fine sand 3. Akleng parang ± Albizia procera (Roxb.) Benth x Place and date of collection: ¾ Camp 3, Tuba, Benguet City Limit, ± Apr-June Ambuklao Road, Baguio City ¾ Mangatarem, Pangasinan ± Mar-April ¾ Ilocos Norte ± April ¾ Manleluag Reforestation, Pangasinan ± Mar-Apr ¾ La Union ± Mar-Apr ¾ Sallacong, Bantay, Ilocos Sur ± Jan-Feb ¾ Diadi, Nueva Vizcaya ± Apr ¾ Caylabne Resort, Cavite ± Mar-Apr ¾ Ternate, Cavite ± Mar-Apr x Seed count: 17,600-25,300/kg x Seed type: Orthodox x Pretreatment: Soak seeds in boiling water for 1 minute and keep soaked in tap water for 24 hours x Sowing medium: 1:1:1 garden soil, dried humus, and fine sand. 4. Lumbang ± Aleurites moluccana (L.) Willd. x Place and date of collection: ¾ Botanic Garden, UPLB ± July-Aug ¾ Diadi, Nueva Vizcaya ± Aug ¾ Valmayorville, College, Laguna ± Feb x Seed count: 116/kg x Seed type: Orthodox x Pretreatment: Cracking of seed coat and soaking in tap water overnight Sowing medium: 1:1:1 sand, topsoil, dried organic x matter 4 5. Batino ± Alstonia macrophylla G. Don x Place and date of collection: ¾ Cavinti & Luisiana, Laguna ± July-Sept ¾ Palawan Experimental Station ± Sept-Oct ¾ Camp 7, Minglanilla, Cebu ± Nov ¾ Calamba, Laguna ± Jan-Feb ¾ Biñan, Laguna ± Sept x Seed count: 365,000/kg x Seed type: Orthodox x Pre treatment: None x Sowing medium: Ordinary garden soil, dried organic matter 6. Dita ± Alstonia scholaris (L.) R. Br. var. scholaris x Place and date of collection: ¾ Ilocos Norte ± Jan - Feb ¾ La Union ± Feb - Mar ¾ Caniaw, Taleb, Bantay, Ilocos Sur ± Feb ¾ Echague, Isabela ± Jan ¾ Binahaan, Pagbilao, Quezon ± Aug-Sept ¾ Calauag, Quezon ± May-June ¾ Sooc, Camarines Sur ± June ¾ San Roque, Sorsogon ± July-Sept ¾ Calamba, Laguna ± Jan-Feb x Seed count: 365,000-370,000/kg x Seed type: Orthodox x Pretreatment: None x Sowing medium: 1:1:1 ordinary garden soil, dried humus, and fine sand 7. Palosapis ± Anisoptera thurifera (Blanco) Blume ssp. thurifera x Place and date of collection: ¾ Leyte ± Sept-Oct x Seed count: 830-900/kg x Seed type: Recalcitrant x Pretreatment: Removal of wings before sowing x Sowing medium: Plastic trays with moistened paper towel or seed boxes with fine sand or 10.16cm x 15.24cm (industry specs) plastic bags with 1:1:1 ratio of sand, topsoil, and dried organic matter 5 8. Binayuyu ± Antidesma ghaesembilla Gaertn. var. ghaesembilla x Place and date of collection: ¾ Cawag, Subic, Zambales ± Aug ¾ Castillejos, Zambales ± July ¾ San Juan, Cabangan, Zambales ± May ¾ San Marcelina, Zambales ± July ¾ Angat, Norzagaray, Bulacan ± July ¾ Mayantoc, Tarlac ± July-Aug ¾ Arayat, Pampanga ± Aug ¾ Tanawan, Dingalan, Quezon ± July-Aug ¾ Palawan Experimental Forest, ± July-Aug Bagumbayan, Napsan, Palawan x Seed count: 78,277/kg x Seed type: Orthodox x Pretreatment: None x Sowing medium: Fine sand or in mixture of garden soil and dried humus. 9. Alibangbang ± Piliostigma malabarica Roxb. x Place and date of collection: ¾ Ilocos Norte ± Apr ¾ Manleluag Reforestation, Pangasinan ± Feb - Apr ¾ La Union ± Mar - Apr ¾ Caniaw and Taleb, Bantay, Ilocos Sur ± Feb ¾ Diadi, Nueva Vizcaya ± Apr ¾ Mt. Makiling, Laguna ± Apr - May ¾ Leyte ± June - July x Seed count: 12,000-12,200/kg x Seed type: Orthodox x Pretreatment: Nicking or cutting of seed coat and soaking in tap water overnight or soaking of seeds in boiling water until water turns cold x Sowing medium: 1:1:1 ratio of ordinary garden soil, dried humus, and fine sand 10. Bitaog ± Calophyllum inophyllum L. x Place and date of collection: ¾ Ilocos Norte ± Jan ¾ Burgos, Bangui ± Mar-Apr ¾ La Union ± Dec ¾ Laongen, Sto. Domingo; Barbar, San Juan; ± Dec Nagkolloban, Sinait, Ilocos Sur ¾ Sooc, Camarines Sur ± Sept-Oct ¾ Legaspi ± Dec-Feb ¾ Camp 7, Minglanilla, Cebu ± July ¾ Leyte and Samar ± Feb-Mar x Seed count: 100-210/kg x Seed type: Intermediate x Pretreatment: Removal of the shell completely or partially then soaking the seeds in tap water overnight x Sowing medium: 1:1:1 ratio of ordinary garden soil, dried humus, and fine sand in plastic trays or seed boxes 6 11. Ilang -ilang ± Cananga odorata (Lamk.) Hook. f. & Thoms. x Place and date of collection: ¾ Binga, Itogon, Benguet ± Aug-Sept ¾ Mangatarem, Pangasinan ± Sept ¾ Region II ± Jan-Dec ¾ Tarlac Province ± Sept-Oct ¾ Ternate, Cavite ± Aug-Sept ¾ Sapian, Capiz ± June-Aug x Seed count: 12,300-12,400/kg x Seed type: Orthodox x Pretreatment: None x Sowing medium: Mixture of fine sand, dried organic matter, and ordinary garden soil(1:1:1) 12. Agoho ± Casuarina equisetifolia L. x Place and date of collection: ¾ ISU, Cabagan, Isabela ± Aug ¾ Bulacan (NLEX) ± June-Aug ¾ Laguna ± June-July ¾ Palawan Experimental Forest,± June-July Sta. Monica, Palawan ¾ Legaspi ± Aug-Oct ¾ Leyte ± Aug x Seed count: 44,260/100g x Seed type: Orthodox x Pretreatment: None x Sowing medium: Fine sand or 1:1:1 ratio of sand, topsoil and dried organic matter 13. Kamagong ± Diospyros discolor Willd. x Place and date of collection: ¾ Bangued, Abra ± Aug-Sept ¾ Dupaz, Nueva Vizcaya ± Aug ¾ Claveria, Cagayan ± June ¾ Mt. Makiling, Los Baños, Laguna ± July-Sept x Seed count: 238/kg x Seed type: Recalcitrant x Pretreatment: Not necessary x S owing medium: Fine river sand in plastic trays or seedbeds or in 10.16cm x 15.24cm (industry specs) plastic bags with 1:1:1 ratio of sand, topsoil, and dried organic matter 7 14. Panau ± Dipterocarpus gracilis Blume x Place and date of collection: ¾ Palawan Experimental Forest ± June-July x Seed count: 145-200/kg x Seed type: Recalcitrant x Pretreatment: None x Sowing medium: Mixture of 1:1:1 ratio of fine sand, dried organic matter and ordinary garden soil 15. Apitong ± Dipterocarpus grandiflorus Blanco x Place and date of collection: ¾ Palawan Experimental Forest ± Aug-Sept ¾ Leyte ± Sept-Oct ¾ Valma, Compostela Valley ± Sept x Seed count: 63/kg (large size); 93/kg (medium size) x Seed type: Recalcitrant x Pretreatment: Removal of wings before sowing x Sowing medium: Plastic trays or seedbeds with fine sand or in 12.7cm x 20.32cm (industry specs) plastic bags with 1:1:1 ratio of sand, topsoil, and dried organic matter 16. Dao ± Dracontomelon dao (Blanco) Merr. & Rolfe x Place and date of collection: ¾ Caniaw, Taleb, Bantay, Ilocos Sur ± Sept-Oct ¾ Diadi, Nueva Vizcaya ± Sept-Oct ¾ Mt. Makiling, Los Baños, Laguna ± Sept-Oct ¾ Leyte and Samar ± Aug ¾ CARAGA ± July-Sept x Seed count: 1,525/kg x Seed type: Orthodox x Pretreatment: Scraping of seed coat and soaking in tap water overnight (for small volume of seeds) or just soak in tap water overnight (for large volume of seeds) x Sowing medium: Fine river sand in plastic trays or seedbeds or in 10.16cm x 15.24cm (industry specs) plastic bags with 1:1:1 ratio of sand, topsoil, and dried organic matter 8 17. Igyo ± Dysoxylum gaudichaudianum (A. Juss.) Miq. x Place and date of collection: ¾ Mt. Makiling, Los Baños, Laguna ± Dec-Jan ¾ Calauag, Quezon ± Dec-Jan ¾ Albay ± Nov-Dec x Seed count: 1,536/kg x Seed type: Intermediate x Pretreatment: Removal of seed coat and soaking in 0.02% fungicide solution overnight x Sowing medium: Mixture of ordinary garden soil, dried humus, and fine sand (1:1:1) 18. Earpod ± Enterolobium cyclocarpum (Jacq.) Griseb. x Place and date of collection: ¾ Magat, Diadi, Nueva Vizcaya ± Apr-May x Seed count: 1,059/kg x Seed type: Orthodox x Pretreatment: Nicking or cutting of seed coat and soaking in tap water or in 0.02% fungicide solution overnight x Sowing medium: Fine river sand in plastic trays or seedbeds or in 10.16cm x 15.24cm (industry specs) plastic bags with 1:1:1 ratio of sand, topsoil, and dried organic matter ± 19.
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