For Research Use Only

NSUN2 Polyclonal Catalog Number:20854-1-AP Featured Product 23 Publications www.ptglab.com

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 20854-1-AP BC001041 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 500 μg/ml by 54888 WB 1:500-1:8000 Nanodrop and 307 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:2000 method using BSA as the standard; NOL1/NOP2/Sun domain family, for WB Source: member 2 IHC 1:50-1:500 IF 1:10-1:100 Rabbit Calculated MW: Isotype: 767 aa, 86 kDa IgG Observed MW: Immunogen Catalog Number: 90-100 kDa AG14791

Applications Tested Applications: Positive Controls: IF, IHC, IP, WB, ELISA WB : HEK-293, COLO 320 cells, HeLa cells, HepG2 cells, Cited Applications: MCF-7 cells, SH-SY5Y cells IF, IHC, IP, WB IP : HeLa cells, Species Specificity: IHC : human breast cancer tissue, human testis tissue human Cited Species: IF : Hela cells, HepG2 cells human, mouse Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing ), is a which Background Information catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Notable Publications Author Pubmed ID Journal Application Yuan Gao 31487418 Cancer Sci WB,IHC Mayumi Okamoto 25233213 PLoS Genet WB Lixiao Chen 34048786 Exp Cell Res WB

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

Immunohistochemical analysis of paraffin- WB result of NSUN2 antibody (20854-1-AP, 1:5000) Immunofluorescent analysis of Hela cells, using embedded human breast cancer tissue slide using with si-Control and si-NSUN2 transfected HepG2 NSUN2 antibody 20854-1-AP at 1:25 dilution and 20854-1-AP (NSUN2 antibody) at dilution of 1:200 cells. Rhodamine-labeled goat anti-rabbit IgG (red). (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-NSUN2 (IP:20854-1-AP, 4ug; Detection:20854-1-AP 1:1000) with HeLa cells lysate 4000ug.