Bacterial Involvements in Ulcerative Colitis: Molecular and Microbiological Studies
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UNIVERSIDADE ESTADUAL DE CAMPINAS Instituto De Biologia
UNIVERSIDADE ESTADUAL DE CAMPINAS Instituto de Biologia SANDERSON TARCISO PEREIRA DE SOUSA INVESTIGAÇÃO DE GENES ENVOLVIDOS NA BIODEGRADAÇÃO DE HIDROCARBONETOS AROMÁTICOS A PARTIR DO METAGENOMA DE MANGUEZAL IMPACTADO COM PETRÓLEO INVESTIGATION OF GENES INVOLVED IN THE BIODEGRADATION OF AROMATIC HYDROCARBONS FROM THE OIL-IMPACTED MANGROVE METAGENOME Campinas 2018 SANDERSON TARCISO PEREIRA DE SOUSA INVESTIGAÇÃO DE GENES ENVOLVIDOS NA BIODEGRADAÇÃO DE HIDROCARBONETOS AROMÁTICOS A PARTIR DO METAGENOMA DE MANGUEZAL IMPACTADO COM PETRÓLEO INVESTIGATION OF GENES INVOLVED IN THE BIODEGRADATION OF AROMATIC HYDROCARBONS FROM THE OIL-IMPACTED MANGROVE METAGENOME Tese apresentada ao Instituto de Biologia da Universidade Estadual de Campinas como parte dos requisitos exigidos para a obtenção do título de Doutor em Genética e Biologia Molecular na Área de Genética de Micro-organismos. Thesis presented to the Institute of Biology of the University of Campinas as partial fulfillment of the requirements for the degree of Doctor in Genetics and Molecular Biology in the area of Genetics of Microorganisms. ESTE ARQUIVO DIGITAL CORRESPONDE À VERSÃO FINAL DA TESE DEFENDIDA PELO ALUNO SANDERSON TARCISO PEREIRA DE SOUSA E ORIENTADO PELA DRA. VALÉRIA MAIA MERZEL. Orientador (a): DRA. VALÉRIA MAIA MERZEL Campinas 2018 Campinas, 20/04/2018. COMISSÃO EXAMINADORA Prof.(a) Dr.(a). Valéria Maia Merzel (Presidente) Prof.(a). Dr.(a) Cynthia Canedo da Silva Prof.(a) Dr(a). Tiago Palladino Delforno Prof.(a) Dr(a). Fabiana Fantinatti Garboggini Prof.(a) Dr(a). Geizecler Tomazetto Os membros da Comissão Examinadora acima assinaram a Ata de Defesa, que se encontra no processo de vida acadêmica do aluno. DEDICATÓRIA... Dedico essa tese a minha querida avó Antonina do Carmo Ribeiro, que hoje, infelizmente, já não está entre nós, mas tenho certeza que continua olhando e orando por mim de onde ela estiver. -
Carnitine Metabolism to Trimethylamine by an Unusual Rieske-Type Oxygenase from Human Microbiota
Carnitine metabolism to trimethylamine by an unusual Rieske-type oxygenase from human microbiota Yijun Zhua,1, Eleanor Jamesona,1, Marialuisa Crosattib,1, Hendrik Schäfera, Kumar Rajakumarb, Timothy D. H. Buggc, and Yin Chena,2 aSchool of Life Sciences and cDepartment of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom; and bDepartment of Infection, Immunity, and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom Edited by David W. Russell, University of Texas Southwestern Medical Center, Dallas, TX, and approved January 29, 2014 (received for review September 5, 2013) Dietary intake of L-carnitine can promote cardiovascular diseases in (14, 15). Assigning functions encoded in the human microbiome humans through microbial production of trimethylamine (TMA) using existing databases can be problematic. For example, the and its subsequent oxidation to trimethylamine N-oxide by hepatic Pfam protein database currently contains over 25% of protein flavin-containing monooxygenases. Although our microbiota are re- families with no assigned functions (release 26.0) (19). sponsible for TMA formation from carnitine, the underpinning mo- Lack of functional characterization of key microbial functions lecular and biochemical mechanisms remain unclear. In this study, in our microbiota is exemplified by very recent studies on car- using bioinformatics approaches, we first identified a two-component diovascular diseases (20–23). These studies have shown that the Rieske-type oxygenase/reductase (CntAB) and associated gene human microbiota is responsible for the production of trime- cluster proposed to be involved in carnitine metabolism in repre- thylamine N-oxide (TMAO), which is believed to promote ath- sentative genomes of the human microbiota. CntA belongs to a erogenesis through its interaction with macrophages and lipid group of previously uncharacterized Rieske-type proteins and has metabolism (20–23). -
Daidzein Intake Is Associated with Equol Producing Status Through an Increase in the Intestinal Bacteria Responsible for Equol Production
Article Daidzein Intake Is Associated with Equol Producing Status through an Increase in the Intestinal Bacteria Responsible for Equol Production Chikara Iino 1, Tadashi Shimoyama 2,*, Kaori Iino 3, Yoshihito Yokoyama 3, Daisuke Chinda 1, Hirotake Sakuraba 1, Shinsaku Fukuda 1 and Shigeyuki Nakaji 4 1 Department of Gastroenterology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan; [email protected] (C.I.); [email protected] (D.C.); [email protected] (H.S.); [email protected] (S.F.) 2 Aomori General Health Examination Center, Aomori 030-0962, Japan 3 Department of Obstetrics and Gynecology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan; [email protected] (K.I.); [email protected] (Y.Y.) 4 Department of Social Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan; [email protected] * Correspondence: [email protected]; Tel.: +81-017-741-2336 Received: 9 January 2019; Accepted: 7 February 2019; Published: 19 February 2019 Abstracts: Equol is a metabolite of isoflavone daidzein and has an affinity to estrogen receptors. Although equol is produced by intestinal bacteria, the association between the status of equol production and the gut microbiota has not been fully investigated. The aim of this study was to compare the intestinal bacteria responsible for equol production in gut microbiota between equol producer and non-producer subjects regarding the intake of daidzein. A total of 1044 adult subjects who participated in a health survey in Hirosaki city were examined. The concentration of equol in urine was measured by high-performance liquid chromatography. -
Upper and Lower Case Letters to Be Used
Isolation, characterization and genome sequencing of a soil-borne Citrobacter freundii strain capable of detoxifying trichothecene mycotoxins by Rafiqul Islam A Thesis Presented to The University of Guelph In Partial Fulfilment of Requirements for the Degree of Doctor of Philosophy in Plant Agriculture Guelph, Ontario, Canada © Rafiqul Islam, April, 2012 ABSTRACT ISOLATION, CHARACTERIZATION AND GENOME SEQUENCING OF A SOIL- BORNE CITROBACTER FREUNDII STRAIN CAPABLE OF DETOXIFIYING TRICHOTHECENE MYCOTOXINS Rafiqul Islam Advisors: University of Guelph, 2012 Dr. K. Peter Pauls Dr. Ting Zhou Cereals are frequently contaminated with tricthothecene mycotoxins, like deoxynivalenol (DON, vomitoxin), which are toxic to humans, animals and plants. The goals of the research were to discover and characterize microbes capable of detoxifying DON under aerobic conditions and moderate temperatures. To identify microbes capable of detoxifying DON, five soil samples collected from Southern Ontario crop fields were tested for the ability to convert DON to a de-epoxidized derivative. One soil sample showed DON de-epoxidation activity under aerobic conditions at 22-24°C. To isolate the microbes responsible for DON detoxification (de-epoxidation) activity, the mixed culture was grown with antibiotics at 50ºC for 1.5 h and high concentrations of DON. The treatments resulted in the isolation of a pure DON de-epoxidating bacterial strain, ADS47, and phenotypic and molecular analyses identified the bacterium as Citrobacter freundii. The bacterium was also able to de-epoxidize and/or de-acetylate 10 other food-contaminating trichothecene mycotoxins. A fosmid genomic DNA library of strain ADS47 was prepared in E. coli and screened for DON detoxification activity. However, no library clone was found with DON detoxification activity. -
Enterorhabdus Caecimuris Sp. Nov., a Member of the Family
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central International Journal of Systematic and Evolutionary Microbiology (2010), 60, 1527–1531 DOI 10.1099/ijs.0.015016-0 Enterorhabdus caecimuris sp. nov., a member of the family Coriobacteriaceae isolated from a mouse model of spontaneous colitis, and emended description of the genus Enterorhabdus Clavel et al. 2009 Thomas Clavel,1 Wayne Duck,2 Ce´dric Charrier,3 Mareike Wenning,4 Charles Elson2 and Dirk Haller1 Correspondence 1Biofunctionality, ZIEL – Research Center for Nutrition and Food Science, Technische Universita¨t Thomas Clavel Mu¨nchen (TUM), 85350 Freising Weihenstephan, Germany [email protected] 2Division of Gastroenterology and Hepatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA 3NovaBiotics Ltd, Aberdeen, AB21 9TR, UK 4Microbiology, ZIEL – Research Center for Nutrition and Food Science, TUM, 85350 Freising Weihenstephan, Germany The C3H/HeJBir mouse model of intestinal inflammation was used for isolation of a Gram- positive, rod-shaped, non-spore-forming bacterium (B7T) from caecal suspensions. On the basis of partial 16S rRNA gene sequence analysis, strain B7T was a member of the class Actinobacteria, family Coriobacteriaceae, and was related closely to Enterorhabdus mucosicola T T Mt1B8 (97.6 %). The major fatty acid of strain B7 was C16 : 0 (19.1 %) and the respiratory quinones were mono- and dimethylated. Cells were aerotolerant, but grew only under anoxic conditions. Strain B7T did not convert the isoflavone daidzein and was resistant to cefotaxime. The results of DNA–DNA hybridization experiments and additional physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain B7T from the type strain of E. -
WO 2018/064165 A2 (.Pdf)
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/064165 A2 05 April 2018 (05.04.2018) W !P O PCT (51) International Patent Classification: Published: A61K 35/74 (20 15.0 1) C12N 1/21 (2006 .01) — without international search report and to be republished (21) International Application Number: upon receipt of that report (Rule 48.2(g)) PCT/US2017/053717 — with sequence listing part of description (Rule 5.2(a)) (22) International Filing Date: 27 September 2017 (27.09.2017) (25) Filing Language: English (26) Publication Langi English (30) Priority Data: 62/400,372 27 September 2016 (27.09.2016) US 62/508,885 19 May 2017 (19.05.2017) US 62/557,566 12 September 2017 (12.09.2017) US (71) Applicant: BOARD OF REGENTS, THE UNIVERSI¬ TY OF TEXAS SYSTEM [US/US]; 210 West 7th St., Austin, TX 78701 (US). (72) Inventors: WARGO, Jennifer; 1814 Bissonnet St., Hous ton, TX 77005 (US). GOPALAKRISHNAN, Vanch- eswaran; 7900 Cambridge, Apt. 10-lb, Houston, TX 77054 (US). (74) Agent: BYRD, Marshall, P.; Parker Highlander PLLC, 1120 S. Capital Of Texas Highway, Bldg. One, Suite 200, Austin, TX 78746 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. -
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INDEX CHAPTER NUMBER CHAPTER NAME PAGE Extraction of Fungal Chitosan and its Chapter-1 1-17 Advanced Application Isolation and Separation of Phenolics Chapter-2 using HPLC Tool: A Consolidate Survey 18-48 from the Plant System Advances in Microbial Genomics in Chapter-3 49-80 the Post-Genomics Era Advances in Biotechnology in the Chapter-4 81-94 Post Genomics era Plant Growth Promotion by Endophytic Chapter-5 Actinobacteria Associated with 95-107 Medicinal Plants Viability of Probiotics in Dairy Products: A Chapter-6 Review Focusing on Yogurt, Ice 108-132 Cream, and Cheese Published in: Dec 2018 Online Edition available at: http://openaccessebooks.com/ Reprints request: [email protected] Copyright: @ Corresponding Author Advances in Biotechnology Chapter 1 Extraction of Fungal Chitosan and its Advanced Application Sahira Nsayef Muslim1; Israa MS AL-Kadmy1*; Alaa Naseer Mohammed Ali1; Ahmed Sahi Dwaish2; Saba Saadoon Khazaal1; Sraa Nsayef Muslim3; Sarah Naji Aziz1 1Branch of Biotechnology, Department of Biology, College of Science, AL-Mustansiryiah University, Baghdad-Iraq 2Branch of Fungi and Plant Science, Department of Biology, College of Science, AL-Mustansiryiah University, Baghdad-Iraq 3Department of Geophysics, College of remote sensing and geophysics, AL-Karkh University for sci- ence, Baghdad-Iraq *Correspondense to: Israa MS AL-Kadmy, Department of Biology, College of Science, AL-Mustansiryiah University, Baghdad-Iraq. Email: [email protected] 1. Definition and Chemical Structure Biopolymer is a term commonly used for polymers which are synthesized by living organisms [1]. Biopolymers originate from natural sources and are biologically renewable, biodegradable and biocompatible. Chitin and chitosan are the biopolymers that have received much research interests due to their numerous potential applications in agriculture, food in- dustry, biomedicine, paper making and textile industry. -
Radionuclide Fate in Naturally Occurring Radioactive Materials (NORM) in the Oil and Gas Industry
Radionuclide Fate in Naturally Occurring Radioactive Materials (NORM) in the Oil and Gas Industry A thesis submitted to the University of Manchester for the degree of Doctor of Philosophy in the Faculty of Science and Engineering 2019 Faraaz Ahmad School of Earth and Environmental Sciences 1 Table of Contents List of Figures…………………………….……………………………………………………………………………………….7 List of Tables…..……………………………..…………………………………………………………………………………15 List of Abbreviations ............................................................................................................. 17 Thesis Abstract ...................................................................................................................... 20 Declaration ............................................................................................................................ 22 Copyright statement ............................................................................................................. 22 Acknowledgements ............................................................................................................... 23 About The Author .................................................................................................................. 25 CHAPTER 1: Introduction…………………………………………..……………………….26 1.0 Project Introduction ................................................................................................... 26 1.1 Aims and objectives ............................................................................................... 31 1.1.1 -
Citrobacter Braakii
& M cal ed ni ic li a l C G f e Trivedi et al., J Clin Med Genom 2015, 3:1 o n l o a m n r DOI: 10.4172/2472-128X.1000129 i u c s o Journal of Clinical & Medical Genomics J ISSN: 2472-128X ResearchResearch Article Article OpenOpen Access Access Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1, Gopal Nayak1, Sambhu Charan Mondal2 and Snehasis Jana2* 1Trivedi Global Inc., Eastern Avenue Suite A-969, Henderson, NV, USA 2Trivedi Science Research Laboratory Pvt. Ltd., Chinar Fortune City, Hoshangabad Rd., Madhya Pradesh, India Abstract Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes. First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of C. -
Phenotyping and 16S Rdna Analysis After Biofield
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana To cite this version: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al.. Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen. Journal of Clinical & Medical Genomics, Omics Publishing Group, 2015, 3 (1), pp.1000129. hal-01435926 HAL Id: hal-01435926 https://hal.archives-ouvertes.fr/hal-01435926 Submitted on 16 Jan 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License & M cal ed ni ic li a l C G f e Trivedi et al., J Clin Med Genom 2015, 3:1 o n l o a m n r DOI: 10.4172/2472-128X.1000129 i u c s o Journal of Clinical & Medical Genomics J ISSN: 2472-128X ResearchResearch Article Article OpenOpen Access Access Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1, Gopal Nayak1, Sambhu Charan Mondal2 and Snehasis Jana2* 1Trivedi Global Inc., Eastern Avenue Suite A-969, Henderson, NV, USA 2Trivedi Science Research Laboratory Pvt. -
Microbial Populations of Fresh and Cold Stored Donkey Milk by High-Throughput Sequencing Provide Indication for a Correct Management of This High-Value Product
applied sciences Communication Microbial Populations of Fresh and Cold Stored Donkey Milk by High-Throughput Sequencing Provide Indication for A Correct Management of This High-Value Product Pasquale Russo 1,*, Daniela Fiocco 2 , Marzia Albenzio 1, Giuseppe Spano 1 and Vittorio Capozzi 3 1 Department of Sciences of Agriculture, Food, and Environment, University of Foggia, via Napoli 25, 71122 Foggia, Italy; [email protected] (M.A.); [email protected] (G.S.) 2 Department of Clinical and Experimental Medicine, University of Foggia, Viale Pinto 1, 71122 Foggia, Italy; daniela.fi[email protected] 3 Institute of Sciences of Food Production, National Research Council (CNR), c/o CS-DAT, Via Michele Protano, 71121 Foggia, Italy; [email protected] * Correspondence: [email protected] Received: 12 March 2020; Accepted: 25 March 2020; Published: 28 March 2020 Abstract: Donkey milk is receiving increasing interest due to its attractive nutrient and functional properties (but also cosmetic), which make it a suitable food for sensitive consumers, such as infants with allergies, the immunocompromised, and elderly people. Our study aims to provide further information on the microbial variability of donkey milk under cold storage conditions. Therefore, we analysed by high-throughput sequencing the bacterial communities in unpasteurized donkey milk just milked, and after three days of conservation at 4 ◦C, respectively. Results showed that fresh donkey milk was characterized by a high incidence of spoilage Gram-negative bacteria mainly belonging to Pseudomonas spp. A composition lower than 5% of lactic acid bacteria was found in fresh milk samples, with Lactococcus spp. being the most abundant. -
Tree Scale: 1 D Bacteria P Desulfobacterota C Jdfr-97 O Jdfr-97 F Jdfr-97 G Jdfr-97 S Jdfr-97 Sp002010915 WGS ID MTPG01
d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Thermodesulfobacterium s Thermodesulfobacterium commune WGS ID JQLF01 d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Thermosulfurimonas s Thermosulfurimonas dismutans WGS ID LWLG01 d Bacteria p Desulfobacterota c Desulfofervidia o Desulfofervidales f DG-60 g DG-60 s DG-60 sp001304365 WGS ID LJNA01 ID WGS sp001304365 DG-60 s DG-60 g DG-60 f Desulfofervidales o Desulfofervidia c Desulfobacterota p Bacteria d d Bacteria p Desulfobacterota c Desulfofervidia o Desulfofervidales f Desulfofervidaceae g Desulfofervidus s Desulfofervidus auxilii RS GCF 001577525 1 001577525 GCF RS auxilii Desulfofervidus s Desulfofervidus g Desulfofervidaceae f Desulfofervidales o Desulfofervidia c Desulfobacterota p Bacteria d d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfatatoraceae g Thermodesulfatator s Thermodesulfatator atlanticus WGS ID ATXH01 d Bacteria p Desulfobacterota c Desulfobacteria o Desulfatiglandales f NaphS2 g 4484-190-2 s 4484-190-2 sp002050025 WGS ID MVDB01 ID WGS sp002050025 4484-190-2 s 4484-190-2 g NaphS2 f Desulfatiglandales o Desulfobacteria c Desulfobacterota p Bacteria d d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g QOAM01 s QOAM01 sp003978075 WGS ID QOAM01 d Bacteria p Desulfobacterota c BSN033 o UBA8473 f UBA8473 g UBA8473 s UBA8473 sp002782605 WGS