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Relevance of the Epigenetic Regulation Exercised by Hepatic Micrornas in the Fatty Liver Arena: from the Bedside to the Bench

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Relevance of the Epigenetic Regulation Exercised by Hepatic Micrornas in the Fatty Liver Arena: from the Bedside to the Bench RELEVANCE OF THE EPIGENETIC REGULATION EXERCISED BY HEPATIC MICRORNAS IN THE FATTY LIVER ARENA: FROM THE BEDSIDE TO THE BENCH Jèssica Latorre Luque Per citar o enllaçar aquest document: Para citar o enlazar este documento: Use this url to cite or link to this publication: http://hdl.handle.net/10803/671499 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. DOCTORAL THESIS RELEVANCE OF THE EPIGENETIC REGULATION EXERCISED BY HEPATIC microRNAs IN THE FATTY LIVER ARENA: from the bedside to the bench Jèssica Latorre Luque 2019 DOCTORAL THESIS RELEVANCE OF THE EPIGENETIC REGULATION EXERCISED BY HEPATIC microRNAs IN THE FATTY LIVER ARENA: from the bedside to the bench Jèssica Latorre Luque 2019 Doctoral Programme in Molecular Biology, Biomedicine and Health Supervised by: Francisco José Ortega Delgado, Ph. D José Manuel Fernández-Real Lemos, Ph. D, M.D. Tutor: Prof. José Manuel Fernández-Real Lemos Presented to obtain the degree of PhD at the University of Girona Dr Francisco José Ortega Delgado, principal investigator of the Biomedical Research Institute of Girona (IDIBGI), Endocrinology, Diabetes and Nutrition Research Unit (UDEN). Researcher ascribed to the CIBER de la Fisiopatologiá de la Obesidad y la Nutricioń (CIBEROBN, ISCIII). Prof. José Manuel Fernández-Real Lemos, group leader of the Biomedical Research Institute of Girona (IDIBGI), Endocrinology, Diabetes and Nutrition Research Unit (UDEN), lead investigator of the CIBER de la Fisiopatologiá de la Obesidad y la Nutricioń (CIBEROBN, ISCIII), and professor of medicine at the University of Girona (UdG). WE DECLARE: That the thesis entitled “RELEVANCE OF THE EPIGENETIC REGULATION EXERCISED BY HEPATIC microRNAs IN THE FATTY LIVER ARENA: from the bedside to the bench”, presented by Jèssica Latorre Luque to obtain the doctoral degree in Molecular Biology, Biomedicine and Health by the UdG, has been completed under our supervision and fulfils all the requirements to qualify for an International Doctorate. For all intents and purposes, we hereby sign this document. Dr. Francisco José Ortega Delgado Dr. José-Manuel Fernández-Real Lemos Girona, 2019 Alça el vol, marxa lluny, I què importa si ningú ho entén? Que les mateixes pors que ens ceguen Són finestres plenes de llum. Oques Grasses (2019) ACKNOWLEDGEMENTS Bé, doncs finalment sembla que he acabat la tesi. La veritat és que abans de començar, només de pensar que hauria de doctorar-me, feia bastanta por perquè no dir-ho. I després de quasi cinc anys, puc dir que m’han passat volant i tot i que hem passat moments bons i no tan bons, tot el que m’enduc no ho canviaria per res. Al final, el trajecte no ha estat tan complicat gràcies al suport i la cooperació incondicional de totes i cadascuna de les persones que m’he trobat pel camí. En primer lloc, vull donar les gràcies al Dr. José Manuel Fernández-Real, director d’aquesta tesi, per ser el primer en parar-me la mà, per la confiança dipositada en mi i per ajudar-me a créixer com a investigadora. Paco, gràcies per tot. Podria fer una llista molt llarga de tot el que m’has ensenyat i com he arribat a aprendre al teu costat. Gràcies per fer-me veure que la ciència pot ser a vegades frustrant, però també molt gratificant. Gràcies per dir-me sempre les veritats, pels consells, per ajudar-me a calmar els nervis, per fer possible que avui acabi aquesta tesi. Gràcies per ser més que un director, un amic. José, el teu suport, els teus consells, la teva disposició a ajudar-me en tot moment i la dedicació que has posat en ensenyar-me fan que ara sigui aquí, a punt de dipositar una tesi que sense vosaltres no hagués estat possible. Quan vaig arribar a UDEN el grup era molt petit. Isa, estar contigo los primeros días cuando empecé me ayudó muchísimo. Després va tornar la Moni, m’havien dit que eres una peça clau del grup i no s’equivocaven. Pacient, enèrgica, amable, atenta, tot un suport i un exemple a seguir. Aina i Ferran, els meus germans petits. Un gran tàndem dins i fora del laboratori, per la tranquil·litat que m’ha donat saber que éreu sempre allà. Hem compartit moltíssimes coses, des dels mítics esmorzars de bikini al Trueta, colze amb colze als tres ordinadors de baix ben apretadets, infinites converses arreglant el món, hores i hores de poiata, i fins a unes bones nits de festa. De les persones amb qui més hem plorat de riure. Aina, tot i que hem passat un any separades, t’he sentit més a prop que mai. Em considero molt afortunada d’haver-vos tingut al costat tot aquest temps. Núria i Anna, les noves incorporacions de UDEN, gràcies per l’ajuda desinteressada, els somriures i les estones juntes. Anna, especial menció pels últims dies colze a colze amb les respectives tesis, pels riures que només denotaven estrés, tu a punt de llegir, i jo estirant-me els i cabells amb els últims detalls. I Núria, per ser els meus segons ulls, per cedir-me una mica del teu perfeccionisme. Gràcies a totes dues, en poc temps hem creat un vincle molt especial. I a tots els membres de UDEN, que han format part de tot això, Estefania, Òscar, Emili, María, Laia, Fer, Luz...gràcies. També agrair a tots els col·laboradors que han format part d’aquest projecte, gràcies a tots per proporcionar les eines necessàries per arrodonir aquesta tesi. Però com que a l’IdIBGi som pocs i benavinguts, em toca agrair una miqueta més. Rocío, crec que vas ser la primera persona que vaig conèixer fora de UDEN, de seguida vaig pensar, serem amigues. Molts consells, però sobretot una pila de bromes per no parar mai de riure. María! Que haría yo sin ti! Creyendo en mi más de lo que yo haré nunca. Gracias por tu apoyo incondicional, por preocuparte siempre por mi y por lo bien que lo hemos pasado juntas, y junto a Aina, las tres. Marini, l’alegria assegurada, un riure contagiós que et canvia l’estat d’ànim fins i tot quan se’t moren les cèl·lules a cultius. Gràcies per transmetre aquesta alegria sempre. I a les noietes de Pedi, Berta i Ari, tant semblants entre vosaltres i molt semblants a mi també, gràcies per la complicitat, pels moments de desconnexió durant el dia, cafès, dinars i estonetes a la tarda si és necessari. Una estona de riures i una mica de xocolata sempre fa les coses més fàcils. També vull agrair-vos Bet, la veu de l’experiència, Sara, Carme, Sílvia, Ester, Esther, Gerard, Laia...per l’agradable convivència de l’IdIBGi. Moltes gràcies a tots. Moving to Finland, I am totally grateful to Prof. Vesa Olkkonen for providing me the opportunity to work in his laboratory for three months. I really appreaciate all the scientific help and facilities. I sincerely thank also to Dr.
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