DOCSLIB.ORG

Download Full Article in PDF Format

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Download Full Article in PDF Format cryptogamie Mycologie 2021 ● 42 ● 4 DIRECTEUR DE LA PUBLICATION / PUBLICATION DIRECTOR : Bruno DAVID Président du Muséum national d’Histoire naturelle RÉDACTEUR EN CHEF / EDITOR-IN-CHIEF : Bart BUYCK ASSISTANTE DE RÉDACTION / ASSISTANT EDITOR : Marianne SALAÜN ([email protected]) MISE EN PAGE / PAGE LAYOUT : Marianne SALAÜN RÉDACTEURS ASSOCIÉS / ASSOCIATE EDITORS : Slavomír ADAMČÍK Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava (Slovakia) André APTROOT ABL Herbarium, G.v.d. Veenstraat 107, NL-3762 XK Soest (The Netherlands) Cony DECOCK Mycothèque de l’Université catholique de Louvain, Earth and Life Institute, Microbiology, Université catholique de Louvain, Croix du Sud 3, B-1348 Louvain-la-Neuve (Belgium) André FRAITURE Botanic Garden Meise, Domein van Bouchout, B-1860 Meise (Belgium) Kevin D. HYDE School of Science, Mae Fah Luang University, 333 M. 1 T.Tasud Muang District, Chiang Rai 57100 (Thailand) Valérie HOFSTETTER Station de recherche Agroscope Changins-Wädenswil, Dépt. Protection des plantes, Mycologie, CH-1260 Nyon 1 (Switzerland) Sinang HONGSANAN College of Life Science and Oceanography, Shenzhen University, 1068, Nanhai Avenue, Nanshan, ShenZhen 518055 (China) Egon HORAK Schlossfeld 17, A-6020 Innsbruck (Austria) Jing LUO Department of Plant Biology & Pathology, Rutgers University New Brunswick, NJ 08901 (United States) Ruvishika S. JAYAWARDENA Center of Excellence in Fungal Research, Mae Fah Luang University, 333 M. 1 T.Tasud Muang District, Chiang Rai 57100 (Thailand) Chen JIE Instituto de Ecología, Xalapa 91070, Veracruz (México) Sajeewa S.N. MAHARCHCHIKUMBURA Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University (Oman) Pierre-Arthur MOREAU UE 7144. Faculté des Sciences pharmaceutiques et biologiques. Université Lille Nord de France. F-59006 Lille (France) Tian QING Center of Excellence in Fungal Research, Mae Fah Luang University 333 M. 1 T.Tasud Muang District, Chiang Rai 57100 (Thailand) Sylvie RAPIOR Laboratoire de Botanique, Phytochimie et Mycologie / UMR -CNRS 5175 CEFE, Faculté de Pharmacie, 15, avenue Charles-Flahault, Université Montpellier I, BP 14491, 34093 Montpellier Cedex 5 (France) Franck RICHARD Université de Montpellier II, CEFE/CNRS Campus du CNRS, 1919, route de Mende, 34293 Montpellier Cedex 5 (France) Naritsada THONGKLANG Center of Excellence in Fungal Research, Mae Fah Luang University, 333 M. 1 T.Tasud Muang District, Chiang Rai 57100 (Thailand) Xiang-Hua WANG CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Kunming 650201, P. R. (China) COUVERTURE / COVER : Extraits d’éléments de la Figure 4 / Extracts of the Figure 4 Cryptogamie, Mycologie est indexé dans / Cryptogamie, Mycologie is indexed in: – Biological Abstracts – Current Contents – Science Citation Index – Publications bibliographiques du CNRS (Pascal). Cryptogamie, Mycologie est distribué en version électronique par / Cryptogamie, Mycologie is distributed electronically by: – BioOne® (http://www.bioone.org/loi/crym) Cryptogamie, Mycologie est une revue en flux continu publiée par les Publications scientifiques du Muséum, Paris Cryptogamie, Mycologie is a fast track journal published by the Museum Science Press, Paris Les Publications scientifiques du Muséum publient aussi / The Museum Science Press also publishes: Adansonia, Geodiversitas, Zoosystema, Anthropozoologica, European Journal of Taxonomy, Naturae, Cryptogamie sous-sections Algologie, Bryologie, Comptes Rendus Palevol. Diffusion – Publications scientifiques Muséum national d’Histoire naturelle CP 41 – 57 rue Cuvier F-75231 Paris cedex 05 (France) Tél. : 33 (0)1 40 79 48 05 / Fax : 33 (0)1 40 79 38 40 [email protected] / http://sciencepress.mnhn.fr © Publications scientifiques du Muséum national d’Histoire naturelle, Paris, 2021 ISSN (imprimé / print) : 0181-1584/ ISSN (électronique / electronic) : 1776-100 Mucoralean fungi in Thailand: novel species of Absidia from tropical forest soil Vedprakash G. HURDEAL Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100 and School of Science, Mae Fah Luang University, Chiang Rai, 57100 (Thailand) Eleni GENTEKAKI School of Science, Mae Fah Luang University, Chiang Rai, 57100 (Thailand) [email protected] (corresponding author) Hyang B. LEE Environmental Microbiology Lab., Dept. of Agricultural Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186 (Korea) Rajesh JEEWON Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit (Mauritius) Kevin D. HYDE Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100 (Thailand) and Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Haizhu District, Guangzhou 510225 (China) and CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650201, Yunnan (China) Saowaluck TIBPROMMA Peter E. MORTIMER Jianchu XU CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650201, Yunnan and Mountain futures, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan (China) Submitted on 13 May 2020 | Accepted on 15 February 2021 | Published on 2 April 2021 Hurdeal V. G., Gentekaki E., Lee H. B., Jeewon R., Hyde K. D., Tipbromma S., Mortimer P. E. & Xu J. 2021. — Mucor- alean fungi in Thailand: novel species of Absidia from tropical forest soil. Cryptogamie, Mycologie 42 (4): 39-61. https:// doi.org/10.5252/cryptogamie-mycologie2021v42a4. http://cryptogamie.com/mycologie/42/4 ABSTRACT Species of Absidia Tiegh. (Mucorales) are commonly isolated from soil, dead plant materials and dung. The genus is of interest in industrial and medical fields due to the presence of active compounds and secondary metabolites. Absidia species are typically characterized by an arcuate stolon with rhizoids, zygospores with appendaged suspensors and columella with apical projections. Characterization and CRYPTOGAMIE, MYCOLOGIE • 2021 • 42 (4) © Publications scientifiques du Muséum national d’Histoire naturelle, Paris. www.cryptogamie.com/mycologie 39 Hurdeal V. G. et al. description of novel Absidia species are unprecedented in Thailand. During our field visits to explore soil fungi in northern Thailand, topsoil samples from a tropical mixed forest in Chiang Mai were collected. Four strains of Absidia were isolated from the soil samples. Initial morphological charac- teristics revealed that four strains represent two new species. Phylogenetic analysis of combined ITS, SSU, LSU, and ACT-1 sequence data revealed that the two new species are phylogenetically distinct. Microscopic investigations indicated that the new species, Absidia soli V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. produces larger sporangia (16-51 × 15-45.5 μm) and columella (7.5- 12.5 × 9-24 μm) than Absidia edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. (30.5-35.5 × 24-27 μm and 5-9.5 × 6.5-20 μm respectively). Both species have short cylindrical to cylindrical and hyaline sporangiospores. The sizes of the spores forA. edaphica sp. nov. and A. soli KEY WORDS sp. nov. are 3.5-5.5 × 2-3.5 μm and 3-4.5 × 1.5-2.5 μm respectively. Mycelial growth in four media, Mucorales, namely malt extract agar (MEA), potato dextrose agar (PDA), corn meal agar (CMA) and yeast malt polyphasic approach, agar (YMA) were measured. Both species grow in the range of 4 to 30°C, but not at >37°C. DNA phylogeny, soil, sequence analyses based on wider taxon sampling and LSU dataset revealed that there is a high phy- new species. logenetic diversity of species of Absidia. RÉSUMÉ Les microchampignons mucorales de Thaïlande : nouvelles espèces d’Absidia du sol de forêt tropicale. Les espèces d’Absidia Tiegh. (Mucorales) sont généralement isolées du sol, des matières végétales mortes et des excréments. Le genre présente un intérêt dans les domaines industriel et médical en raison de la présence de composés actifs et de métabolites secondaires. Les espèces Absidia sont typiquement carac- térisées par un stolon arqué avec des rhizoïdes, des zygospores avec des suspenseurs appendus et des columelles avec des projections apicales. La caractérisation et la description des nouvelles espèces d’Absidia sont sans précédent en Thaïlande. Au cours de nos visites sur le terrain pour explorer les champignons du sol dans le nord de la Thaïlande, des échantillons de terre végétale ont été prélevés dans une forêt tropicale mixte à Chiang Mai. Quatre souches Absidia ont été isolées à partir des échantillons de sol. Les caractéristiques morphologiques initiales ont révélé que quatre souches représentent deux nouvelles espèces. L’analyse phylogénétique des données de séquences combinées ITS, SSU, LSU et ACT-1 a révélé que les deux nouvelles espèces sont phylogénétiquement distinctes. L’examen microscopique a indiqué que la nouvelle espèce Absidia soli V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. produit des sporanges (16-51 × 15-45,5 μm) et des columelles (7,5-12,5 × 9-24 μm) plus grands que Absidia edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. (30,5-35,5 × 24-27 μm et 5-9,5 × 6,5-20 μm respectivement). Les deux espèces ont des sporangiospores cylindriques courts à
Load more

Recommended publications
  • Molecular Identification of Fungi
    Molecular Identification of Fungi Youssuf Gherbawy l Kerstin Voigt Editors Molecular Identification of Fungi Editors Prof. Dr. Youssuf Gherbawy Dr. Kerstin Voigt South Valley University University of Jena Faculty of Science School of Biology and Pharmacy Department of Botany Institute of Microbiology 83523 Qena, Egypt Neugasse 25 [email protected] 07743 Jena, Germany [email protected] ISBN 978-3-642-05041-1 e-ISBN 978-3-642-05042-8 DOI 10.1007/978-3-642-05042-8 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2009938949 # Springer-Verlag Berlin Heidelberg 2010 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg, Germany, kindly supported by ‘leopardy.com’ Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Dedicated to Prof. Lajos Ferenczy (1930–2004) microbiologist, mycologist and member of the Hungarian Academy of Sciences, one of the most outstanding Hungarian biologists of the twentieth century Preface Fungi comprise a vast variety of microorganisms and are numerically among the most abundant eukaryotes on Earth’s biosphere.
    [Show full text]
  • Absidia Corymbifera
    Publié sur INSPQ (https://www.inspq.qc.ca) Accueil > Expertises > Santé environnementale et toxicologie > Qualité de l’air et rayonnement non ionisant > Qualité de l'air intérieur > Compendium sur les moisissures > Fiches sur les moisissures > Absidia corymbifera Absidia corymbifera Absidia corymbifera [1] [2] [3] [4] [5] [6] [7] Introduction Laboratoire Métabolites Problèmes de santé Milieux Diagnostic Bibliographie Introduction Taxonomie Règne Fungi/Metazoa groupe Famille Mucoraceae (Mycocladiaceae) Phylum Mucoromycotina Genre Absidia (Mycocladus) Classe Zygomycetes Espèce corymbifera (corymbiferus) Ordre Mucorales Il existe de 12 à 21 espèces nommées à l’intérieur du genre Absidia, selon la taxonomie utilisée {1056, 3318}; la majorité des espèces vit dans le sol {431}. Peu d’espèces sont mentionnées comme pouvant être trouvées en environnement intérieur {2694, 1056, 470}. Absidia corymbifera, aussi nommé Absidia ramosa ou Mycocladus corymbiferus, suscite l’intérêt, particulièrement parce qu’il peut être trouvé en milieu intérieur et qu’il est associé à plusieurs problèmes de santé. Même si l’espèce Absidia corymbifera a été placée dans un autre genre (Mycocladus) par certains taxonomistes, elle sera traitée dans ce texte comme étant un Absidia et servira d’espèce type d’Absidia. Absidia corymbifera est l’espèce d’Absidiala plus souvent isolée et elle est la seule espèce reconnue comme étant pathogène parmi ce genre, causant une zygomycose (ou mucoromycose) chez les sujets immunocompromis; il faut noter que ces zygomycoses sont souvent présentées comme une seule entité, étant donné que l’identification complète de l’agent n’est confirmée que dans peu de cas. Quelques-unes des autres espèces importantes d’Absidia sont : A.
    [Show full text]
  • Universidade De Évora Caracterização Polifásica
    UNIVERSIDADE DE ÉVORA ESCOLA DE CIÊNCIAS E TECNOLOGIA DEPARTAMENTO DE QUÍMICA CARACTERIZAÇÃO POLIFÁSICA DE FUNGOS FILAMENTOSOS ZYGOMYCETES COM INTERESSE EM BIOTECNOLOGIA AMBIENTAL Vânia Patrícia Gonçalves Dantas Orientação: Prof.ª Doutora Maria do Rosário Martins Prof. Doutor Nelson Lima Mestrado em Bioquímica Dissertação Évora, 2015 UNIVERSIDADE DE ÉVORA ESCOLA DE CIÊNCIAS E TECNOLOGIA DEPARTAMENTO DE QUÍMICA CARACTERIZAÇÃO POLIFÁSICA DE FUNGOS FILAMENTOSOS ZYGOMYCETES COM INTERESSE EM BIOTECNOLOGIA AMBIENTAL Vânia Patrícia Gonçalves Dantas Orientação: Prof.ª Doutora Maria do Rosário Martins Prof. Doutor Nelson Lima Mestrado em Bioquímica Dissertação Évora, 2015 AGRADECIMENTOS Este espaço é dedicado aqueles que de alguma forma contribuíram para que este trabalho fosse realizado. Foram muitas as pessoas que contribuíram direta e indiretamente para o sucesso do mesmo e, assim sendo, deixo aqui o meu profundo e sincero agradecimento. À Professora Maria do Rosário Martins pela orientação, ensinamentos, amizade e ajuda prestada durante a realização deste trabalho. Ao Professor Nelson Lima pela orientação, ensinamentos, compreensão, simpatia, bem como por proporcionar a realização de parte do trabalho na Micoteca da Universidade do Minho. À Doutora Célia Soares, pela paciência, apoio, dedicação e sobretudo amizade prestada, bem como a forma como me recebeu e me integrou no Departamento de Engenharia Biológica. À Anabela Cabeça, do Departamento de Química da Universidade de Évora, que me acompanhou desde o início do trabalho e que me transmitiu todo o incentivo necessário para a continuação e finalização do mesmo, que me motivou ao máximo pela sua boa disposição e por toda a alegria e carinho transmitidos. A todas as colegas de laboratório, mais especificamente a Andreia Piçarra, Carolina Rufino e Sílvia Arantes, que também contribuíram para a realização do trabalho, dando todos os apoios, tanto a nível do trabalho em si, como também a nível emocional, tornando os dias mais divertidos e descontraídos.
    [Show full text]
  • Ecology of Fungi in Wildland Soils Along the Mauna Loa Transect
    Technical Report No. 75 ECOLOGY OF FUNGI IN WILDLAND SOILS ALONG THE MAUNA LOA TRANSECT Martin F. Stoner Department of Biological Sciences California State Polytechnic University Pomona, California 91768 Darleen K. Stoner Walnut Valley Unified School District Walnut, California 91789 Gladys E. Baker Department of Botany University of Hawaii Honolulu, Hawaii 96822 ISIJU~D ECOSYSTEMS IRP • U. S. International Biological Program November 1975 ABSTRACT The distribution of fungi in soils along the Mauna Loa Transect was deter­ mined by an approach employing specific fungal reference genera, selective isolation methods, and a combination of analytical techniques. Two sets of transect ~ones were determined on the basis of fungal distribution. The influence of environmental factors, particularly those relating to soil, vascular plant communities, and climate, are interpreted according to distribution patterns. The distribution of fungal groups coincided clearly with vascular plant communities of the transect as defined by other studies. Features of the structure, stability, and development of fungal communities, and of the ecological roles of certain fungi are indicated by the results. The composition, spatial distribution, and environmental relationships of fungal communities along the Mauna Loa Transect are compared with situations in other insular and continental ecosystems in order to further characterize and elucidate the ecology of the Hawaiian soil-borne mycoflora. An overall evaluation of the research indicates that the selective methods
    [Show full text]
  • Transformation of the Fungus Absidia Glauca By
    FEBS Open Bio 2 (2012) 197–201 journal homepage: www.elsevier.com/locate/febsopenbio Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene Sedighe Karimi, Jana Wetzel, Johannes Wostemeyer,¨ Anke Burmester* General Microbiology and Microbe Genetics, Friedrich-Schiller University Jena, Neugasse 24, D-07743 Jena, Germany article info abstract Article history: Transformation of fungi by complementation of auxotrophs is generally much more reliable than Received 25 April 2012 usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Received in revised form 6 July 2012 Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the Accepted 6 July 2012 minus mating type and characterized at the molecular level. The mutant is disrupted in the coding region of the Met2-1 gene, encoding homoserine O-acetyltransferase. The corresponding wild type gene Keywords: was cloned, sequenced and inserted into appropriate vector plasmids. Transformants are prototrophs Homoserine O-acetyltransferase Met2 and show restored methionine-independent growth, based on complementation by the autonomously Methionine biosynthesis replicating plasmids. Mucor-like fungi c 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Zygomycetes 1. Introduction The most intriguing feature of P. parasitica infection is the transfer of nuclei from the parasite to the host, followed by establishment of Over the past two decades, the mucoralean fungus Absidia glauca foreign genes in the host. This phenomenon that occurs in frequencies has been developed to an important model for studying genetics and between 10−3 and 10−2 with respect to the total numbers of uninucle- molecular biology of the biotrophic fusion parasitism between the ated sporangiospores on the Petri dish used for infection, was proven host organism A.
    [Show full text]
  • Producing Fungi in Phylum Mucoromycota
    Master’s Thesis 2020 60 ECTS Faculty of Biosciences (BIOVIT) Whole genome sequencing with Oxford Nanopore and de novo genome assemblies of lipid- producing fungi in phylum Mucoromycota Kai Fjær Master of Biotechnology, Genetics Summary Phylum Mucoromycota consists of economically and ecologically important fungi, including industrial producers of lipids, enzymes and fermented foods and beverages, symbionts and decomposers of plants, as well as fungi causing post-harvest diseases and opportunistic infections in humans. The phylum includes great candidates for sustainable production lipids, but very few have so far been the subject of genomic research. In this project, the genomes of eleven lipid-producing strains were sequenced and assembled and placed phylogenetically in the phylum. Genomic DNA was extracted using bead-beating and sequenced with Oxford Nanopore Technologies’ PromethION platform. Three barcoded sequencing runs produced 62.7 gigabases and 18.9 million reads, of which 13 million reads were used to create de novo assemblies. Extracting high-quality DNA from Mucoromycota fungi is challenging. When extracting DNA for long-read sequencing, care should be taken to avoid mechanical fragmentation of the DNA and to inactivate and inhibit DNA-degrading enzymes. Despite the comparatively short read lengths resulting from degradation of the extracted DNA, the nanopore reads resulted in highly contiguous assemblies for several strains. 2 / 90 Abbreviations DNA Deoxyribose nucleic acid EDTA Ethylenediaminetetraacetic acid SDS Sodium
    [Show full text]
  • PORTADA Puente Biologico
    ISSN1991-2986 RevistaCientíficadelaUniversidad AutónomadeChiriquíenPanamá Polyporus sp.attheQuetzalestrailintheVolcánBarúNationalPark,Panamá Volume1/2006 ChecklistofFungiinPanama elaboratedinthecontextoftheUniversityPartnership ofthe UNIVERSIDAD AUTÓNOMA DECHIRIQUÍ and J.W.GOETHE-UNIVERSITÄT FRANKFURT AMMAIN supportedbytheGerman AcademicExchangeService(DAAD) For this publication we received support by the following institutions: Universidad Autónoma de Chiriquí (UNACHI) J. W. Goethe-Universität Frankfurt am Main German Academic Exchange Service (DAAD) German Research Foundation (DFG) Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ)1 German Federal Ministry for Economic Cooperation and Development (BMZ)2 Instituto de Investigaciones Científicas Avanzadas 3 y Servicios de Alta Tecnología (INDICASAT) 1 Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ) GmbH Convention Project "Implementing the Biodiversity Convention" P.O. Box 5180, 65726 Eschborn, Germany Tel.: +49 (6196) 791359, Fax: +49 (6196) 79801359 http://www.gtz.de/biodiv 2 En el nombre del Ministerio Federal Alemán para la Cooperación Económica y el Desarollo (BMZ). Las opiniones vertidas en la presente publicación no necesariamente reflejan las del BMZ o de la GTZ. 3 INDICASAT, Ciudad del Saber, Clayton, Edificio 175. Panamá. Tel. (507) 3170012, Fax (507) 3171043 Editorial La Revista Natura fue fundada con el objetivo de dar a conocer las actividades de investigación de la Facultad de Ciencias Naturales y Exactas de la Universidad Autónoma de Chiriquí (UNACHI), pero COORDINADORADE EDICIÓN paulatinamente ha ampliado su ámbito geográfico, de allí que el Comité Editorial ha acordado cambiar el nombre de la revista al Clotilde Arrocha nuevo título:PUENTE BIOLÓGICO , para señalar así el inicio de una nueva serie que conserva el énfasis en temas científicos, que COMITÉ EDITORIAL trascienden al ámbito internacional. Puente Biológico se presenta a la comunidad científica Clotilde Arrocha internacional con este número especial, que brinda los resultados Pedro A.CaballeroR.
    [Show full text]
  • Mucorales) Richard K
    Aliso: A Journal of Systematic and Evolutionary Botany Volume 5 | Issue 3 Article 2 1963 Obligate Azygospore Formation in Two Species of Mucor (Mucorales) Richard K. Benjamin B. S. Mehrotra Follow this and additional works at: http://scholarship.claremont.edu/aliso Part of the Botany Commons Recommended Citation Benjamin, Richard K. and Mehrotra, B. S. (1963) "Obligate Azygospore Formation in Two Species of Mucor (Mucorales)," Aliso: A Journal of Systematic and Evolutionary Botany: Vol. 5: Iss. 3, Article 2. Available at: http://scholarship.claremont.edu/aliso/vol5/iss3/2 ALISO VoL. 5, No.3, pp. 235-245 APRIL 15, 1963 OBLIGATE AZYGOSPORE FORMATION IN TWO SPECIES OF MUCOR (MUCORALES) RICHARD K. BENJAMIN1 AND B.S. MEHROTRA2 In the course of sexual reproduction in many Mucorales, gametangia! copulation occasion­ ally fails to take place normally and one or both of the gametangia involved in the sexual act may give rise to a parthenospore or azygospore. Azygospores typically are morphologically like the true zygospores of the species in which they occur, although they may be somewhat smaller. They may be single or double depending on whether or not one or both of the opposed gametangia develop parthenogenetically (for typical examples see Lead beater & Mercer, 1957a, Pl. 5, fig. 7~9, and 1957b, fig. 4 g~h). In many instances, according to Ling­ Young (1930), presumed double azygospores may be only true zygospores in which the exospore [ zygosporangium 1 differentiates prior to complete fusion of the gametangia. As Ling-Young states in the discussion of the azygospore included in his classic work on sexuality in the Mucorales (Ling-Young, 1930, pp.
    [Show full text]
  • Understanding Germination and Pathogenicity in Zygomycota Species Through Genomic and Transcriptomic Approaches by Poppy Sephton Clark
    Understanding Germination and Pathogenicity in Zygomycota Species through Genomic and Transcriptomic Approaches by Poppy Sephton Clark A thesis submitted to the University of Birmingham for the degree of DOCTOR OF PHILOSOPHY School of Biosciences College of Life and Environmental Sciences University of Birmingham August 2019 University of Birmingham Research Archive e-theses repository This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. Abstract Mucorales spores are the causative agents of the emerging disease mucormycosis. Mucorales species are also responsible for high quantities of food spoilage annually. The mechanism by which Mucorales spores cause disease and rot relies upon spore germination, however the mechanism underlying germination in these species remains poorly understood. Presented here are results which characterise Mucorales spore germination, through phenotypic and transcriptional studies (RNA-Seq), which followed the defined germination phenotype throughout. Hallmark pathways are identified through analysis of differentially expressed genes and co-transcriptional networks, providing targets for germination inhibition. With the resulting transcriptional data, the genome of Rhizopus delemar was enriched and analysed, thus providing better information on the Mucoralean genome. Comparative genomics was also employed to better understand genotypic variation between Mucorales species.
    [Show full text]
  • The Family Structure of the Mucorales: a Synoptic Revision Based on Comprehensive Multigene-Genealogies
    Persoonia 30, 2013: 57–76 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X666259 The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies K. Hoffmann1,2, J. Pawłowska3, G. Walther1,2,4, M. Wrzosek3, G.S. de Hoog4, G.L. Benny5*, P.M. Kirk6*, K. Voigt1,2* Key words Abstract The Mucorales (Mucoromycotina) are one of the most ancient groups of fungi comprising ubiquitous, mostly saprotrophic organisms. The first comprehensive molecular studies 11 yr ago revealed the traditional Mucorales classification scheme, mainly based on morphology, as highly artificial. Since then only single clades have been families investigated in detail but a robust classification of the higher levels based on DNA data has not been published phylogeny yet. Therefore we provide a classification based on a phylogenetic analysis of four molecular markers including the large and the small subunit of the ribosomal DNA, the partial actin gene and the partial gene for the translation elongation factor 1-alpha. The dataset comprises 201 isolates in 103 species and represents about one half of the currently accepted species in this order. Previous family concepts are reviewed and the family structure inferred from the multilocus phylogeny is introduced and discussed. Main differences between the current classification and preceding concepts affects the existing families Lichtheimiaceae and Cunninghamellaceae, as well as the genera Backusella and Lentamyces which recently obtained the status of families along with the Rhizopodaceae comprising Rhizopus, Sporodiniella and Syzygites. Compensatory base change analyses in the Lichtheimiaceae confirmed the lower level classification of Lichtheimia and Rhizomucor while genera such as Circinella or Syncephalastrum completely lacked compensatory base changes.
    [Show full text]
  • Fungal Contaminants in Drinking Water Regulation? a Tale of Ecology, Exposure, Purification and Clinical Relevance
    Perspective Fungal Contaminants in Drinking Water Regulation? A Tale of Ecology, Exposure, Purification and Clinical Relevance Monika Novak Babič 1,*, Nina Gunde-Cimerman 1, Márta Vargha 2, Zsófia Tischner 3, Donát Magyar 4, Cristina Veríssimo 5, Raquel Sabino 5,6, Carla Viegas 6, Wieland Meyer 7 and João Brandão 8,* 1 Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia; [email protected] 2 Department of Water Hygiene, National Public Health Center, Albert Flórián út 2-6, H-1097 Budapest, Hungary; [email protected] 3 Department of Biology, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary; [email protected] 4 Department of Air Hygiene and Aerobiology, National Public Health Center, Albert Flórián út 2-6, H-1097 Budapest, Hungary; [email protected] 5 Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal; [email protected] (C.V.); [email protected] (R.S.) 6 GIAS, ESTeSL—Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, 1990-096 Lisbon, Portugal; [email protected] 7 Molecular Mycology Research Laboratory, Centre for Infectious Disease and Microbiology, Sydney Medical School, Westmead Hospital, Marie Bashir Institute for Emerging Infectious Diseases and Biosecurity, Westmead Institute for Medical Research, The University of Sydney, Level 4, Room 0.4.04, 176 Hawkesbury Road, Westmead, NSW 2145, Australia; [email protected] 8 Department of Environmental Health, National Institute of Health Doutor Ricardo Jorge, Av.
    [Show full text]
  • The Family Structure of the <I>Mucorales</I>: a Synoptic
    UvA-DARE (Digital Academic Repository) The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies Hoffmann, K.; Pawłowska, J.; Walther, G.; Wrzosek, W.; de Hoog, G.S.; Benny, G.L.; Kirk, P.M.; Voigt, K. DOI 10.3767/003158513X666259 Publication date 2013 Document Version Final published version Published in Persoonia - Molecular Phylogeny and Evolution of Fungi Link to publication Citation for published version (APA): Hoffmann, K., Pawłowska, J., Walther, G., Wrzosek, W., de Hoog, G. S., Benny, G. L., Kirk, P. M., & Voigt, K. (2013). The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies. Persoonia - Molecular Phylogeny and Evolution of Fungi, 30, 57-76. https://doi.org/10.3767/003158513X666259 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl) Download date:05 Oct 2021 Persoonia 30, 2013: 57–76 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X666259 The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies K.
    [Show full text]