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Mafr De Enterococcus Faecalis Y Mgap De Streptococcus Pneumoniae

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Mafr De Enterococcus Faecalis Y Mgap De Streptococcus Pneumoniae UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE CIENCIAS BIOLÓGICAS TESIS DOCTORAL Caracterización de dos activadores transcripcionales: MafR de Enterococcus faecalis y MgaP de Streptococcus pneumoniae Characterization of two transcriptional activators: MafR of Enterococcus faecalis and MgaP of Streptococcus pneumoniae MEMORIA PARA OPTAR AL GRADO DE DOCTORA PRESENTADA POR Ana Moreno Blanco DIRECTORA Alicia Bravo García Madrid © Ana Moreno Blanco, 2020 UNIVERSIDAD COMPLUTENSE DE MADRID Facultad de Ciencias Biológicas Departamento de Bioquímica y Biología Molecular TESIS DOCTORAL Caracterización de dos activadores transcripcionales: MafR de Enterococcus faecalis y MgaP de Streptococcus pneumoniae Characterization of two transcriptional activators: MafR of Enterococcus faecalis and MgaP of Streptococcus pneumoniae Ana Moreno Blanco Directora: Alicia Bravo García Madrid, 2020 UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE CIENCIAS BIOLÓGICAS TESIS DOCTORAL CARACTERIZACIÓN DE DOS ACTIVADORES TRANSCRIPCIONALES: MafR DE Enterococcus faecalis Y MgaP DE Streptococcus pneumoniae CHARACTERIZATION OF TWO TRANSCRIPTIONAL ACTIVATORS: MafR OF Enterococcus faecalis AND MgaP OF Streptococcus pneumoniae MEMORIA PARA OPTAR AL GRADO DE DOCTOR PRESENTADA POR ANA MORENO BLANCO DIRECTORA ALICIA BRAVO GARCÍA CENTRO DE INVESTIGACIONES BIOLÓGICAS MARGARITA SALAS CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC) MADRID, 2020 A mi madre, mi padre y mis hermanos. “Que no sea inmortal puesto que es llama, pero que sea eterno mientras dure” Vinicius de Moraes Poco espacio para expresar todo lo que llevo dentro. Ciertas palabras se repiten una y otra vez a lo largo de estos agradecimientos, "orgullo", "confianza", "vida", "motivar"... No es falta de léxico, es ser afortunada por haberos encontrado en mi camino. A ti, Alicia, por tu infinita paciencia y buen hacer. Por darme la oportunidad y confiar en mí. Muchas gracias por tu eterna dedicación y todas las horas que has invertido en mi formación. Gracias por tu generosidad y por todas las celebraciones que hemos podido disfrutar, y por las que espero poder seguir celebrando. La vida está hecha de momentos, así que vive. Manolo, por tu inmensa sabiduría, tus ganas de aprender y sobre todo, tus ganas de "chinchar" y meter el gusanillo de la curiosidad en los cerebritos que te vas encontrando. A los dos, “más, porque mejor es imposible”. No podría haber aprendido en un sitio mejor. Sofi, gracias por todo lo que me has enseñado tanto como persona como profesionalmente. Qué bonito que nuestra amistad empezase con el flow del DuoFlow, se fortaleciese con el Ac. Acético y 32P, y continuara con ¡¡Atrévete-te!! Queda pendiente el burocracy-scaperoom, y todo lo que aún no hemos descubierto. Aunque no ha sido posible, para mí siempre serás mi Co-directora, y sin duda, mi couch-animadora-motivadora. Sin ti y sin Alicia, no lo hubiera conseguido. Itzi, a ti por tus ganas de vida. Gracias por motivar a todo aquel que se cruza en tu camino, por ofrecer todo de ti y dar hasta las entrañas, por ser como eres, y enseñar como enseñas. A Glori y Paloma, por dejar que me acercara a vuestras mentes, que aunque son muy diferentes entre sí, son de lo más maravilloso del país. Glori por tu infinito apoyo, ánimo e inteligencia. Paloma por tu curiosidad, por acogerme como otra más del labo y por compartirme tu amor y experiencias por la ciencia. Josecin, echaré de menos pisarte tus zapatos impolutos y patearte por debajo de la mesa. Muchas gracias por aguantarme y escucharme; y sobre todo por responder siempre con una carcajada al "¿Tenéis comidita? - me muero de hambre", cuando aparecía en vuestro laboratorio a altas horas de la tarde (también a Glori, mi oficial proveedora de pastas y quesos, y a "GSD management"). Rafica, gracias por tu enorme y constante sonrisa que le mejora el día a cualquiera, por tener siempre una buena palabra en la boca y estar ahí con tus “¡Qué pasa Anica!”. Javi, también conocido como CO de GSD-management. Por tus infinitos "Che Ana, Relájate!" y tus incesantes propuestas de planes, eso sí, siempre con la "bucita campera". Diego, por tener siempre palabras bonitas y ser siempre tan "excelente". A Cathy, la chica que más brilla de todo el CIB. Thank you for your support and be always by my side. You are just amazing. Adriancin, por las risas y los bailes que nos hemos echado, que algún día nos lanzarán al estrellato, “porque en España la ciencia está muy mal, y eso ha sido así, de siempre…”; Gracias por el ambiente que creas allí donde vas, siendo el "β-glucano" de la planta tres. Gorettita, por la alegría y energía que desprendes allí donde vas. Norhane, merci beaucoup pour tes sourires et ta paix intérieure. A Mª Luz, Montse, Kenza, Annel, Paula, Inés, Lore, Vero, Jingwen, Virtu y todas las personas que han pasado por el CIB, brindándome su ayuda y dejando en mí su huella. Gracias por los "breaks" por los pasillos o en el comedor. A Rosa y su gente, sois los que habéis estado en mi peor momento de la Tesis. Gracias por confiar en mí y darme la oportunidad de unirme a vuestro mundo en una situación tan delicada. Nunca tendré forma de agradeceros la ayuda, facilidad y alegría que me habéis dado. A mis XXLKS, simplemente por estar siempre, por apoyarme y secuestrarme con un "tómate una cerveza, que eso te quita todo". Gracias por todos los viajes y retiros: por Cadalso, Peguerinos, Xeraco, Cork, Interrail… por todo en general, porque ¡Bastante hemos fregao' ya! Y obviamente, gracias por el soporte, sicológico, técnico, de diseño y administrativo, entre otros mil. Aquí, yo ya cierro mi cuaderno de “aborto”. Os quiero churris. A mis Biologogos, si de algo puedo estar más orgullosa, aparte de haber acabado la carrera, es de haberos conocido y tener este grupo. Lo mejor de la universidad, sin duda, habéis sido vosotros. Desde los “visu-culo” en el metro, pasando por los vivac en la Pedriza hasta las noches por Madrid. Especial mención a Chen, sin ti a saber qué sería de mí. A ti por ser tú, porque tú entero alegras el alma, mi alma. A mi rubito, Javi, por estar siempre en mis sueños y por haberme dejado un huequito en ese corazoncito. Blanditas, gracias por escuchar mis dramas continuos y estar ahí. Quedan pendientes esos viajes que nos cultivan el espíritu. Marina y Ali, con vosotras, un mundo. Marina, por esa mirada que inclina la cabeza y extiende los brazos, para acabar dándome ese abrazo que me levanta y saca del pozo. Siempre con tu sonrisa y ese corazón que tienes que retumba de lo fuerte que late. Ali, por todos los ratos de “cosquis” y por tus constantes ganas de ayudar, gracias. Que tu inquietud y arte por nuevas manualidades no desaparezca nunca, artista. Hajni, the strongest woman in all Hungary. Without you, Lori and Daniela, we had not been able to survive. Thank you for all your love and kind. A Julia que siempre me muestra su admiración, haciendo que confíe más en mi misma. Contigo todo es fácil y simple, y pese a las adversidades siempre sales a flote: “la palmera que se dobla, pero que resiste el huracán”. No puedo estar más orgullosa de ti. Gracias por estar en mi vida. Águeda, porque la distancia nunca fue problema para nosotras. Por ese mes que ha forjado nuestra amistad año tras año. Gracias por incluirme en tu enorme mundo. “Anem a la platja bussejar” y de cenar “algo ligerito”. A Paqui y Manolo, gracias por toda vuestra ayuda y generosidad. Gracias por acogerme como otra hija más. Me siento orgullosa de muchas cosas en mi vida, pero sin duda la que está en el top es mi Familia. Porque aunque nunca acabasteis de entender que es esto, nunca dejasteis de apoyarme y confiar en que lo lograría. A mis tetes por abrirme el camino e ir curtiéndome en la vida. Iván, a ti por tu corazón y por darlo todo. Porque lo que te pedimos se convierte en deseos cumplidos. También por tu cariño y afecto, "Salta pequeño canguro". Cris, gracias por llegar a estar familia y por todo lo que estás haciendo por nosotros. Fran, gracias por tu esfuerzo por querer entender lo que hago y el motivo por el cual lo hago, significa mucho para mí. No se me olvidarán tus palabras recién entrada en la carrera, "sé la mejor en lo que haces, y nunca te faltará trabajo". Quizás nunca llegue a ser la mejor, pero si me seguiré esforzando en conseguirlo, por vosotros y por mí. Gracias por actuar como un muro de carga cuando todo tambalea. A ti Almu, por acogerme como una hermana pequeña y ayudarme siempre que te lo pido. Cuñis, porque realmente pienso que habéis mejorado esta familia. A ti papá, por todas tardes que hemos pasado juntos en la calle, jugando, y no entre cuatro paredes. Por enseñarme la vida, desde habilidades sociales y hasta el amor por los seres vivos. Gracias por tu infinita paciencia y tus "Bueno, no pasa nada" cuando la "liaba". Gracias por todo lo que habéis trabajado tu y mamá para que nunca nos faltase nada a los tetes y a mí. Gracias por enseñarme bondad y respeto. Mamá, por ti, la vida. No conozco a otra persona que esté más orgullosa de sus hijos, pero tampoco conozco a unos hijos que estén más orgullosos de su madre, de ti. Tu forma de querernos, nos protege de todo. Al final, daba igual cual fuera el resultado, tu siempre has estado orgullosa de nosotros por llegar, motivándonos a seguir luchando. Tu también, papá. Gracias por todo lo que has/habéis luchado y trabajado para formar esta familia. Gracias por quitaros el pan de la boca para dárnoslo a nosotros.
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