DOCSLIB.ORG

Bibliometric Analysis of Ongoing Projects 11Th Report September 2020

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Bibliometric Analysis of Ongoing Projects 11Th Report September 2020 Bibliometric analysis of ongoing projects 11th Report September 2020 Copyright ©2020 Innovative Medicines Initiative Prepared by Clarivate on behalf of IMI Programme Office under a public procurement procedure document reference: IMI.2018.OP.01. Bibliometric analysis of IMI ongoing projects 1 Disclaimer/Legal Notice This document has been prepared solely for the Innovative Medicines Initiative (IMI). All contents may not be re-used (in whatever form and by whatever medium) by any third party without prior permission of the IMI. Bibliom etric analysis of IMI ongoing projects 2 TABLE OF CONTENTS 1 EXECUTIVE SUMMARY ................................................................................................................. 5 2 INTRODUCTION ............................................................................................................................. 8 2.1 OVERVIEW ............................................................................................................................. 8 2.2 INNOVATIVE MEDICINES INITIATIVE (IMI) JOINT UNDERTAKING ................................... 8 2.3 CLARIVATE ............................................................................................................................ 8 2.4 SCOPE OF THIS REPORT .................................................................................................... 9 3 DATA SOURCES, INDICATORS AND INTERPRETATION ......................................................... 10 3.1 BIBLIOMETRICS AND CITATION ANALYSIS ..................................................................... 10 3.2 DATA SOURCE .................................................................................................................... 10 3.3 METHODOLOGY .................................................................................................................. 11 3.4 DATA COLLATION ............................................................................................................... 13 4 CITATION ANALYSIS – IMI SUPPORTED PUBLICATIONS OVERALL ...................................... 14 4.1 PUBLICATIONS FROM IMI-SUPPORTED PROJECTS ...................................................... 14 4.2 PUBLICATIONS FROM IMI PROJECTS BY DOCUMENT TYPE ........................................ 16 4.3 TRENDS IN PUBLICATION OUTPUT .................................................................................. 17 4.4 PUBLICATION OUTPUT BY COUNTRY .............................................................................. 19 4.5 PUBLICATION OUTPUT BY IMI PROJECT ......................................................................... 22 4.6 IS IMI PROJECT RESEARCH WELL-CITED? ..................................................................... 24 4.7 IN WHICH JOURNALS DO IMI PROJECT PUBLICATIONS APPEAR MOST FREQUENTLY? .............................................................................................................................................. 26 4.8 WHICH RESEARCH FIELDS ACCOUNT FOR THE HIGHEST VOLUME OF IMI PROJECT PUBLICATIONS? .............................................................................................................................. 30 4.9 IMI RESEARCH FIELDS WITH THE HIGHEST VOLUME OF PUBLICATIONS BENCHMARKED AGAINST EU-28 PUBLICATIONS OF THE SAME FIELD .................................. 35 5 CITATION ANALYSIS – AT IMI PROJECT LEVEL ....................................................................... 37 5.1 TRENDS IN PUBLICATION OUTPUT BY IMI FUNDING CALL .......................................... 37 5.2 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 1 PROJECTS – CALL 1 ....................... 41 5.3 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 1 PROJECTS – CALL 2 ....................... 43 5.4 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 1 PROJECTS – CALL 3 ....................... 45 5.5 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 1 PROJECTS – CALL 4 ....................... 47 5.6 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 1 PROJECTS – CALL 5-10 .................. 49 5.7 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 1 PROJECTS – CALL 11 ..................... 51 5.8 SUMMARY BIBLIOMETRIC ANALYSES FOR IMI 2 PROJECTS ....................................... 53 6 GEOGRAPHIC CLUSTERING ANALYSIS.................................................................................... 56 6.1 LOCATIONS WHERE IMI-FUNDED RESEARCH TAKES PLACE ...................................... 56 7 COLLABORATION ANALYSIS FOR IMI RESEARCH .................................................................. 68 Bibliom etric analysis of IMI ongoing projects 3 7.1 COLLABORATION ANALYSIS FOR IMI RESEARCH ......................................................... 68 7.2 COLLABORATION ANALYSIS BY IMI PROJECT ............................................................... 71 7.3 COLLABORATION METRICS FOR IMI RESEARCH........................................................... 84 7.4 COLLABORATION INDEX .................................................................................................... 94 8 BENCHMARKING ANALYSIS – IMI PROJECT RESEARCH AGAINST RESEARCH FROM SELECTED COMPARATORS .............................................................................................................. 96 8.1 IDENTIFYING COMPARATORS .......................................................................................... 96 8.2 TRENDS IN OUTPUT: IMI PROJECT RESEARCH COMPARED WITH SELECTED COMPARATORS .............................................................................................................................. 97 8.3 SUMMARY OF BIBLIOMETRIC INDICATORS: IMI PROJECT RESEARCH COMPARED WITH SELECTED COMPARATORS .............................................................................................. 112 ANNEX 1: BIBLIOMETRICS AND CITATION ANALYSIS .................................................................. 113 ANNEX 2: BIOMEDICALLY RELATED JOURNAL CATEGORIES .................................................... 122 ANNEX 3: TOTAL NUMBER OF WEB OF SCIENCE PUBLICATIONS FROM IMI PROJECTS BETWEEN 2010 AND 2019 BY COUNTRY ....................................................................................... 123 ANNEX 4: TOTAL NUMBER OF WEB OF SCIENCE PUBLICATIONS, PAPERS AND OPEN- ACCESS PUBLICATIONS BETWEEN 2010 AND 2019 BY PROJECT ............................................ 126 ANNEX 5: COLLABORATION INDEX FOR ALL IMI SUPPORTED RESEARCH PROJECTS ......... 129 ANNEX 6: BIBLIOGRAPHY OF HOT PAPERS AND HIGHLY-CITED PAPERS ............................... 132 Bibliom etric analysis of IMI ongoing projects 4 1 EXECUTIVE SUMMARY This report presents a bibliometric analysis of the Innovative Medicine Initiative Joint Undertaking’s (IMI JU) research published between 2010 and 2019, using citations as an index of academic impact and co-authorship as an index of collaboration. This is the eleventh report commissioned by IMI from Clarivate. The data show that IMI continues to perform well. To date, IMI projects have produced 5,943 publications which have been matched to the Clarivate Web of Science™. This represents a 20% increase from the 4,938 publications matched to the Web of Science in the tenth report, which covered IMI project research published between 2010 and 2018. Between 2010 and 2018, the number of IMI research publications increased year on year. However, in 2019, the most recent year, publication output had fallen slightly. Growth had previously been sustained by an increasing number of active IMI projects and the fact that by some of IMI 1’s longest running and most productive projects (e.g., BTCure) have now come to an end is almost certainly a factor in the observed change in trend. However, this is a single observation and should not necessarily be taken as indicative of a long-term change in trajectory which will depend on the output of new and future IMI 2 projects. The majority of IMI research (55.7%) continues to be published in high impact journals, i.e. those journals in the highest quartile (Q1) ranked by Journal Impact Factor, and the average Journal Impact Factor of all IMI project publications was 6.38. IMI research was wide-ranging from basic biological research to clinical practice. IMI project research has been published most frequently in the fields of Pharmacology & Pharmacy, Neurosciences and Biochemistry & Molecular Biology. The impact of IMI project research (as indicated by citation impact) has remained high, with an increase in citation impact since last year. The field-normalised citation impact of IMI project research (2.05) is over twice the world average (1.00), which indicates the research was internationally influential. Between 2010 and 2019, the field-normalised citation impact of IMI papers was considerably higher (80%) than the European Union’s (EU) average citation impact (1.14) in similar biomedical fields (journal subject categories). Over a quarter of papers from IMI projects were highly cited - that is, the papers were in the world’s top 10% of papers in the same journal category and year of publication, when ranked by number of citations. The output of individual IMI projects has also increased between 2010 and 2019. BTCure (Call 2) has remained the most prolific IMI project, with 680 publications as of this report. This is a 5.4% increase on the 645 publications attributed to BTCure in the previous report. However, this growth is slower than the growth for all IMI projects in aggregate; most likely because the BTCure project ended in early 2017. Projects funded by IMI are highly collaborative. Since the last report, an
Load more

Recommended publications
  • Fiorio Pla Gkika2019 Rev Nohighlights
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Institutional Research Information System University of Turin Ca2+ channels toolkit in Neuroendocrine tumors Alessandra Fiorio Pla1, 2, 3* and Dimitra Gkika2, 3 1 Department of Life Science and Systems Biology, University of Torino, Turin, Italy. 2 Université de Lille, Inserm U1003, PHYCEL laboratory and Villeneuve d’Ascq, France. 3 Laboratory of Excellence, Ion Channels Science and Therapeutics, Université de Lille, Villeneuve d’Ascq, France. Short Title: Ca2+ channels in Neuroendocrine tumors *Corresponding Author Alessandra Fiorio Pla Department of Life Science and Systems Biology University/Hospital Via Accademia Albertina 13 Torino, 10123, Italy Tel: +390116704660 E-mail: [email protected] Keywords: Neuroendocrine tumors, Ca2+ signals, ion channels. Abstract Neuroendocrine tumors (NET) constitute an heterogenous group of malignancies with various clinical presentations and growth rates but all rising from neuroendocrine cells located all over the body. NET present a relatively low frequency disease being mostly represented by gastroenteropancreatic (GEP) and bronchopulmonary tumors (pNET); on the other hand an increasing frequency and prevalence has been associated to NET. Beside the great effort of the latest years, management of NET is still a critical unmet point due to the lack in the knowledge of the biology of the disease, lack of adequate biomarkers, late presentation, the relative insensitivity of imaging modalities and a paucity of predictably 1 effective treatment options. In this context Ca2+ signals, being pivotal molecular devices in sensing and integrating signals from the microenvironment, are emerging to be particularly relevant in cancer, where they mediate interactions between tumor cells and the tumor microenvironment to drive different aspects of neoplastic progression (e.g.
    [Show full text]
  • Potassium Channels in Peripheral Pain Pathways: Expression, Function and Therapeutic Potential
    Send Orders for Reprints to [email protected] Current Neuropharmacology, 2013, 11, 621-640 621 Potassium Channels in Peripheral Pain Pathways: Expression, Function and Therapeutic Potential Xiaona Du1,* and Nikita Gamper1,2,* 1Department of Pharmacology, Hebei Medical University, Shijiazhuang, China; 2Faculty of Biological Sciences, University of Leeds, Leeds, UK Abstract: Electrical excitation of peripheral somatosensory nerves is a first step in generation of most pain signals in mammalian nervous system. Such excitation is controlled by an intricate set of ion channels that are coordinated to produce a degree of excitation that is proportional to the strength of the external stimulation. However, in many disease states this coordination is disrupted resulting in deregulated peripheral excitability which, in turn, may underpin pathological pain states (i.e. migraine, neuralgia, neuropathic and inflammatory pains). One of the major groups of ion channels that are essential for controlling neuronal excitability is potassium channel family and, hereby, the focus of this review is on the K+ channels in peripheral pain pathways. The aim of the review is threefold. First, we will discuss current evidence for the expression and functional role of various K+ channels in peripheral nociceptive fibres. Second, we will consider a hypothesis suggesting that reduced functional activity of K+ channels within peripheral nociceptive pathways is a general feature of many types of pain. Third, we will evaluate the perspectives of pharmacological enhancement of K+ channels in nociceptive pathways as a strategy for new analgesic drug design. + + Keywords: K channel/ M channel/ two-pore K channel/ KATP channel/ Dorsal root ganglion/ Pain/ Nociception. INTRODUCTION TRPV1 [4] while strong mechanical stimulation activates mechanosensitive channels which can be Piezo2 [5].
    [Show full text]
  • The Chondrocyte Channelome: a Novel Ion Channel Candidate in the Pathogenesis of Pectus Deformities
    Old Dominion University ODU Digital Commons Biological Sciences Theses & Dissertations Biological Sciences Summer 2017 The Chondrocyte Channelome: A Novel Ion Channel Candidate in the Pathogenesis of Pectus Deformities Anthony J. Asmar Old Dominion University, [email protected] Follow this and additional works at: https://digitalcommons.odu.edu/biology_etds Part of the Biology Commons, Molecular Biology Commons, and the Physiology Commons Recommended Citation Asmar, Anthony J.. "The Chondrocyte Channelome: A Novel Ion Channel Candidate in the Pathogenesis of Pectus Deformities" (2017). Doctor of Philosophy (PhD), Dissertation, Biological Sciences, Old Dominion University, DOI: 10.25777/pyha-7838 https://digitalcommons.odu.edu/biology_etds/19 This Dissertation is brought to you for free and open access by the Biological Sciences at ODU Digital Commons. It has been accepted for inclusion in Biological Sciences Theses & Dissertations by an authorized administrator of ODU Digital Commons. For more information, please contact [email protected]. THE CHONDROCYTE CHANNELOME: A NOVEL ION CHANNEL CANDIDATE IN THE PATHOGENESIS OF PECTUS DEFORMITIES by Anthony J. Asmar B.S. Biology May 2010, Virginia Polytechnic Institute M.S. Biology May 2013, Old Dominion University A Dissertation Submitted to the Faculty of Old Dominion University in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY BIOMEDICAL SCIENCES OLD DOMINION UNIVERSITY August 2017 Approved by: Christopher Osgood (Co-Director) Michael Stacey (Co-Director) Lesley Greene (Member) Andrei Pakhomov (Member) Jing He (Member) ABSTRACT THE CHONDROCYTE CHANNELOME: A NOVEL ION CHANNEL CANDIDATE IN THE PATHOGENESIS OF PECTUS DEFORMITIES Anthony J. Asmar Old Dominion University, 2017 Co-Directors: Dr. Christopher Osgood Dr. Michael Stacey Costal cartilage is a type of rod-like hyaline cartilage connecting the ribs to the sternum.
    [Show full text]
  • Emerging Roles of the Membrane Potential: Action Beyond the Action Potential
    fphys-09-01661 November 19, 2018 Time: 14:41 # 1 REVIEW published: 21 November 2018 doi: 10.3389/fphys.2018.01661 Emerging Roles of the Membrane Potential: Action Beyond the Action Potential Lina Abdul Kadir1, Michael Stacey2 and Richard Barrett-Jolley1* 1 Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, United Kingdom, 2 Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, United States Whilst the phenomenon of an electrical resting membrane potential (RMP) is a central tenet of biology, it is nearly always discussed as a phenomenon that facilitates the propagation of action potentials in excitable tissue, muscle, and nerve. However, as ion channel research shifts beyond these tissues, it became clear that the RMP is a feature of virtually all cells studied. The RMP is maintained by the cell’s compliment of ion channels. Transcriptome sequencing is increasingly revealing that equally rich compliments of ion channels exist in both excitable and non-excitable tissue. In this review, we discuss a range of critical roles that the RMP has in a variety of cell types Edited by: beyond the action potential. Whereas most biologists would perceive that the RMP is Raheela N. Khan, primarily about excitability, the data show that in fact excitability is only a small part of it. University of Nottingham, United Kingdom Emerging evidence show that a dynamic membrane potential is critical for many other Reviewed by: processes including cell cycle, cell-volume control, proliferation, muscle contraction Juan C. Saez, (even in the absence of an action potential), and wound healing. Modulation of the RMP Pontificia Universidad Católica de Chile, Chile is therefore a potential target for many new drugs targeting a range of diseases and Bruno A.
    [Show full text]
  • Acutemicroglialrespons
    ACUTE MICROGLIAL RESPONSES TO SINGLE NON-EPILEPTOGENIC VS. EPILEPTOGENIC SEIZURES IN MOUSE HIPPOCAMPUS A Dissertation submitted to the Faculty of the Graduate School of Arts and Sciences of Georgetown University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Neuroscience By Alberto Sepulveda-Rodriguez, B.S. Washington, D.C. June 5, 2019 Copyright 2019 by Alberto Sepulveda-Rodriguez All Rights Reserved ii ACUTE MICROGLIAL RESPONSES TO SINGLE NON-EPILEPTOGENIC VS. EPILEPTOGENIC SEIZURES IN MOUSE HIPPOCAMPUS Alberto Sepulveda-Rodriguez, B.S. Thesis Advisor: Stefano Vicini, Ph.D. ABSTRACT As the resident macrophage of the central nervous system, microglia are in a uniquely privileged position to both affect and be affected by neuroinflammation, neuronal activity and injury, which are all hallmarks of seizures and the epilepsies. In one of the prototypical rodent models of seizure-induced epilepsy, hippocampal microglia become activated after prolonged, damaging seizures known as Status Epilepticus (SE). However, since SE comprises both neuronal hyperactivity and injury, the specific mechanisms triggering this microglial activation remain unclear, as does its relevance to the ensuing epileptogenic processes. The present studies employed another well-established seizure model, electroconvulsive shock (ECS), to study the effect of paroxysmal/ictal neuronal hyperactivity on mouse hippocampal microglia, in the absence of concomitant neuronal degeneration. Unlike SE, ECS did not cause neuronal injury and did not alter hippocampal CA1 microglial and astrocytic density, morphology nor baseline process motility. In contrast, both ECS and SE produced a similar increase in ATP-directed microglial process motility in acute slices and similarly upregulated expression of the iii chemokine CCL2.
    [Show full text]
  • Biol. Pharm. Bull. 41(8)
    1126 Biol. Pharm. Bull. 41, 1126 (2018) Vol. 41, No. 8 Current Topics Ion Channels as Therapeutic Targets for the Immune, Inflammatory, and Metabolic Disorders Foreword Susumu Ohya Department of Pharmacology, Graduate School of Medical Sciences, Nagoya City University; Nagoya 467–8601, Japan. A large number of ion channels and their auxiliary subunits osteoarthritis (OA). Recent ‘chondrocyte channelome’ studies play pivotal roles in various cellular signaling networks in have shown that a range of ion channels and transporters are nervous, cardiovascular, immune, metabolic, and endocrine expressed on plasma and intracellular membrane of chondro- systems. The ion channel dysfunctions produce “Channelo- cytes, and regulate multiple intracellular signaling pathways. pathies” such as neural, cardiovascular, immune, metabolic, The third review is “Physiological and Pathological Functions and endocrine disorders, therefore, ion channels are potential of Cl− Channels in Chondrocytes” by Yamamura et al. They therapeutic targets for treatment of their disorders. Review ar- first introduce the cation channels expressed in chondrocytes, ticles under the headings of ‘Ion Channels as Therapeutic Tar- and then review the physiological and pathophysiological roles gets for the Immune, Inflammatory, and Metabolic Disorders’ of Cl− channels/transporters composed of several superfami- will provide new insights and strategies to “Channelopathies.” lies (CFTR, ClC, TMEM16/ANO1). They play important roles Recent findings showed that voltage-gated calcium channels in control of the resting membrane potential, and are critical (VGCC) are responsible for the generation of inflammation to intracellular Ca2+ signaling. This review provides a novel and inflammatory pain. The first review is “Involvement of approach for ameliorating RA and OA severities. The forth Voltage-Gated Calcium Channels in Inflammation and Inflam- review is “Physiological and Pathophysiological Roles of Tran- matory Pain” by Sekiguchi et al.
    [Show full text]
  • A Digital Atlas of Ion Channel Expression Patterns in the Two-Week-Old Rat Brain
    Neuroinform (2015) 13:111–125 DOI 10.1007/s12021-014-9247-0 DATA ORIGINAL ARTICLE A Digital Atlas of Ion Channel Expression Patterns in the Two-Week-Old Rat Brain Volodymyr Shcherbatyy & James Carson & Murat Yaylaoglu & Katharina Jäckle & Frauke Grabbe & Maren Brockmeyer & Halenur Yavuz & Gregor Eichele Published online: 7 October 2014 # The Author(s) 2014. This article is published with open access at Springerlink.com Abstract The approximately 350 ion channels encoded by Keywords Ion channels . Gene expression analysis . In situ the mammalian genome are a main pillar of the nervous hybridization . Genepaint.org database . Rat brain . Digital system. We have determined the expression pattern of 320 atlas . Subdivision mesh channels in the two-week-old (P14) rat brain by means of non- radioactive robotic in situ hybridization. Optimized methods were developed and implemented to generate stringently cor- Introduction onal brain sections. The use of standardized methods permits a direct comparison of expression patterns across the entire ion Ion channels arguably are the functionally most important channel expression pattern data set and facilitates recognizing proteins of the nervous system. Accordingly, there exists a ion channel co-expression. All expression data are made pub- wealth of studies illustrating their spatiotemporal expression lically available at the Genepaint.org database. Inwardly rec- patterns at mRNA and protein levels. Critical knowledge tifying potassium channels (Kir, encoded by the Kcnj genes) about expression patterns includes information on co- regulate a broad spectrum of physiological processes. Kcnj expression of channel auxiliary subunits that form oligomers channel expression patterns generated in the present study and co-expression of channels known to operate in a concert- were fitted with a deformable subdivision mesh atlas produced ed fashion.
    [Show full text]
  • [Frontiers in Bioscience, Scholar, 5, 305-324, January 1, 2013] 305
    [Frontiers in Bioscience, Scholar, 5, 305-324, January 1, 2013] Calcium signalling in chondrogenesis: implications for cartilage repair Csaba Matta1, Roza Zakany1 1Department of Anatomy, Histology and Embryology, University of Debrecen, Medical and Health Science Centre, Nagyerdei krt. 98, H-4032 Debrecen, Hungary TABLE OF CONTENTS 1. Abstract 2. Introduction 3. Adult mesenchymal stem cells in cartilage repair 4. Articular cartilage: from structure to function 5. Chondrogenesis is regulated by interplay between numerous intra- and extracellular factors 6. Calcium signalling: a single messenger with diverse functions 7. Ca2+ entry processes in MSCs and in differentiating or mature chondrocytes 7.1. Voltage-operated Ca2+ entry pathways 7.2. Ligand-operated Ca2+ entry pathways 7.2.1. Purinergic signalling pathways 7.2.2. N-methyl-D-aspartate receptor mediated pathways 7.2.3. Transient Receptor Potential (TRP) pathways 7.3. Ca2+ release from internal stores and store-operated Ca2+ entry 8. Ca2+ elimination processes in MSCs, differentiating and mature chondrocytes 9. Temporal characteristics of Ca2+ dependent signals 9.1. Day-by-day variation of cytosolic Ca2+ concentration 9.2. Ca2+ oscillations in mesenchymal stem cells and chondrocytes 10. Ca2+ signalling during mechanotransduction 11. Conclusions 12. Acknowledgements 13. References 1. ABSTRACT 2. INTRODUCTION Undifferentiated mesenchymal stem cells Prevalence of musculoskeletal disorders (MSCs) represent an important source for cell-based tissue (comprised more than 100 conditions, including various regeneration techniques that require differentiation towards rheumatic, arthritic and joint diseases) is constantly specific lineages, including chondrocytes. Chondrogenesis, increasing owing to unfavourable changes in the population the process by which committed mesenchymal cells of developed countries, exerting an ever-growing burden on differentiate into chondrocytes, is controlled by complex healthcare systems around the globe (1).
    [Show full text]
  • The Chondrocyte Channelome: a Narrative Review
    Joint Bone Spine 86 (2019) 29–35 Available online at ScienceDirect www.sciencedirect.com Review The chondrocyte channelome: A narrative review Ali Mobasheri a,b,c,∗,1, Csaba Matta d, Ilona Uzielienè c, Emma Budd a,1, Pablo Martín-Vasallo e, Eiva Bernotiene c a Department of Veterinary Pre-Clinical Sciences, School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, United Kingdom b Arthritis Research UK Centre for Sport, Exercise and Osteoarthritis, Queen’s Medical Centre, Nottingham, United Kingdom c Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania d Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary e Department of Biochemistry and Molecular Biology, University of La Laguna, Tenerife, Spain article info abstract Article history: Chondrocytes are the main cells in the extracellular matrix (ECM) of articular cartilage and possess a Accepted 31 January 2018 highly differentiated phenotype that is the hallmark of the unique physiological functions of this spe- Available online 13 February 2018 cialised load-bearing connective tissue. The plasma membrane of articular chondrocytes contains a rich and diverse complement of membrane proteins, known as the membranome, which defines the cell Keywords: surface phenotype of the cells. The membranome is a key target of pharmacological agents and is impor- Osteoarthritis tant for chondrocyte function. It includes channels, transporters, enzymes, receptors, and anchors for Articular cartilage intracellular, cytoskeletal and ECM proteins and other macromolecular complexes. The chondrocyte Chondrocyte channelome is a sub-compartment of the membranome and includes a complete set of ion channels and Membranome Channelome porins expressed in these cells.
    [Show full text]
  • VIEWPOINT: the Emerging Fibroblast-Like Synoviocyte Channelome. Simon Tew & Richard Barrett-Jolley [email protected]
    VIEWPOINT: The Emerging Fibroblast-like Synoviocyte Channelome. Simon Tew & RicharD Barrett-Jolley [email protected], [email protected] Synovial joint arthritis is a leading cause of Disability with treatment options being remarkably limiteD. The synovial membrane lines the joint space, proDucing synovial fluiD to facilitate joint lubrication, anD is a tissue richly innervateD with nociceptors. It also acts as a key gateway between the avascular joint space anD the circulatory system. Therefore, as we work towarDs new treatments that target joint disease, building an understanding of how the cells within the synovial membrane are regulateD is of critical importance. SaDly, whilst we Do know a fair amount about the composition anD function of the synovium, we still have a lot to learn about how to control the important fibroblast-like synoviocytes within. The latest stuDy by KonDo et al 2018 goes some way to aDDress this by developing a funDamentally important working hypothesis for control of synoviocytes that links, purinergic anD histamine receptor activation to intracellular calcium mobilisation and cell membrane hyperpolarisation. KonDo et al 2018 use an unusually well laDen tool bag of cell physiological techniques to put this schema together; with a panel of 19 qPCR primer pairs, intracellular Ca2+ imaging, flash photolysis of cageD ip3 anD of course patch-clamp electrophysiology. Two separate inflammatory meDiators, ATP anD histamine both act in parallel to trigger PLC activation, IP3 elevation, thence Ca2+ elevation anD activation of a potassium conDuctance. A particular strength is that the entire moDel is built in human fibroblast-like synoviocytes bringing two strengths over previous moDels, which have tended to be based on either healthy rodent joints or rheumatic human joints.
    [Show full text]
  • Transcriptome-Based Screening of Ion Channels and Transporters in a Migratory Chondroprogenitor Cell Line Isolated from Late-Stage Osteoarthritic Cartilage
    Transcriptome-based screening of ion channels and transporters in a migratory chondroprogenitor cell line isolated from late-stage osteoarthritic cartilage Csaba Matta ( [email protected] ) University of Debrecen https://orcid.org/0000-0002-9678-7420 Rebecca Lewis University of Surrey https://orcid.org/0000-0003-1395-3276 Christopher Fellows University of Surrey Gyula Diszhazi University of Debrecen Janos Almassy University of Debrecen Nicolai Miosge Georg August University James E. Dixon University of Nottingham Marcos C. Uribe University of Nottingham Sean May University of Nottingham Szilard Poliska University of Debrecen Richard Barrett-Jolley University of Liverpool https://orcid.org/0000-0003-0449-9972 Erin Henslee University of Surrey Fatima H. Labeed University of Surrey https://orcid.org/0000-0002-0092-257X Michael P. Hughes University of Surrey Ali Mobasheri University of Oulu https://orcid.org/0000-0001-6261-1286 Page 1/54 Research Article Keywords: Chondrocyte, Cartilage, Mesenchymal stem cell, Osteoarthritis, Chondroprogenitor, Transcriptomics, Channelome Posted Date: November 13th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-106355/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published at Journal of Cellular Physiology on May 18th, 2021. See the published version at https://doi.org/10.1002/jcp.30413. Page 2/54 Abstract Chondrogenic progenitor cells (CPCs) may be used as an alternative source of cells with potentially superior chondrogenic potential compared to mesenchymal stem cells (MSCs), and could be exploited for future regenerative therapies targeting articular cartilage in degenerative diseases such as osteoarthritis (OA).
    [Show full text]
  • Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes
    Int. J. Mol. Sci. 2012, 13, 4433-4445; doi:10.3390/ijms13044433 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes Ismail M. Hdud 1, Abdelrafea A. El-Shafei 2, Paul Loughna 1, Richard Barrett-Jolley 3 and Ali Mobasheri 1,* 1 School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK; E-Mails: [email protected] (I.M.H.); [email protected] (P.L.) 2 Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt; E-Mail: [email protected] 3 Department of Musculoskeletal Biology, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, Merseyside L69 3GA, UK; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +44-115-951-6449; Fax: +44-115-951-6440. Received: 27 February 2012; in revised form: 12 March 2012 / Accepted: 26 March 2012 / Published: 10 April 2012 Abstract: Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV) subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective cation channels whereas TRPV5 and TRPV6 show high selectivity for calcium over other cations. In this study we investigated the effect of time in culture and passage number on the expression of TRPV4, TRPV5 and TRPV6 in articular chondrocytes isolated from equine metacarpophalangeal joints. Polyclonal antibodies raised against TRPV4, TRPV5 and TRPV6 were used to compare the expression of these channels in lysates from first expansion chondrocytes (P0) and cells from passages 1–3 (P1, P2 and P3) by western blotting.
    [Show full text]