DOCSLIB.ORG

Marinomonas Mediterranea. Papel De La Flavoproteína Lodb En La Generación Del Cofactor Quinónico De Loda Mediante Modificación Post-Traduccional

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Marinomonas Mediterranea. Papel De La Flavoproteína Lodb En La Generación Del Cofactor Quinónico De Loda Mediante Modificación Post-Traduccional UNIVERSIDAD DE MURCIA FACULTAD DE BIOLOGÍA Estudio de la Lisina-Épsilon-Oxidasa LodA Sintetizada por Marinomonas mediterranea. Papel de la Flavoproteína LodB en la Generación del Cofactor Quinónico de LodA mediante Modificación Post-Traduccional. Dª. Mª Dolores Chacón Verdú 2015 Powered by TCPDF (www.tcpdf.org) Powered by TCPDF (www.tcpdf.org) El trabajo realizado en la presente memoria ha dado lugar a las siguientes publicaciones: Chacón-Verdú, M. D.; Gómez, D.; Solano, F.; Lucas-Elío, P. and Sánchez-Amat, A. (2014). LodB is required for the recombinant synthesis of the quinoprotein L-lysine- epsilon-oxidase from Marinomonas mediterranea. Appl. Microbiol. Biotechnol. 98(7):2981-2989. doi: 10.1007/s00253-013-5168-3. Chacón-Verdú, M. D.; Campillo-Brocal, J. C.; Lucas-Elío, P.; Davidson, V. L. and Sánchez-Amat, A. (2015). Characterization of recombinant biosynthetic precursors of the cysteine tryptophylquinone cofactors of L-lysine-epsilon-oxidase and glycine oxidase from Marinomonas mediterranea. BBA-Proteins and Proteomics. pii: S1570- 9639(14):00334-3. doi: 10.1016/j.bbapap.2014.12.018. Campillo-Brocal, J. C; Chacón-Verdú, M. D.; Lucas-Elío, P. and Sánchez-Amat, A. (2015). Distribution in microbial genomes of genes similar to lodA and goxA which encode a novel family of quinoproteins with amino acid oxidase activity. BMC Genomics 16(231). doi:10.1186/s12864-015-1455-y. Además ha sido expuesto en los siguientes Congresos Nacionales e Internacionales: . Gordon Research Conference on Protein Cofactors, Radicals & Quinones (2012) Mount Holyoke College, South Hadley, MA (EEUU). OxiZymes 2012. Marsella (Francia). VII Congreso de la Federación Española de Biotecnólogos (BAC2013). Sevilla (España). XIV International Symposium and VII European Conference on Marine Natural Products (MaNaPro) (2013). La Toja (España). I Congreso Internacional Campus Mare Nostrum de Jóvenes Investigadores en el Mediterráneo (2013). Murcia (España). III Jornadas de inicio a la investigación de estudiantes de la Facultad de Biología (2013). Murcia (España). OxiZymes 2014. Viena (Austria). The Fourth International Conference on Cofactors (ICC-04) (2014). Parma (Italia). AGRADECIMIENTOS AGRADECIMIENTOS Con estas palabras quiero dar las gracias a todas aquellas personas que de algún modo han participado en este proyecto. Un agradecimiento especial quiero dedicarle a mi director de tesis Dr. Antonio Sánchez Amat que sin conocerme depositó su confianza en mí y me dio la oportunidad de entrar en el mundo de la investigación. Muchísimas gracias de corazón. A la Dra. Patricia Elío Lucas, que me cedió los mutantes de LodA y que siempre estuvo dispuesta a ayudarme en lo que necesitara. A los Drs. Antonio Romero y Fco. Javier Medrano del CIB-CSIC (Madrid) por su colaboración en la obtención de la estructura recombinante de LodA. Y a este último, gracias por cederme el plásmido pETMBP28. Al Dr. Alejandro Torrecillas del Servicio de Biología Molecular del SAI de la Universidad de Murcia, por su valioso aporte en esta memoria en las diferentes técnicas de espectrometría de masas. A mis compañeros de departamento, Jonatan y Carmen, vuestra presencia, conversaciones y compañía han hecho el pasillo y el laboratorio más agradable todos estos años. Gracias también a Rafa, Sandra y Aída con los que en algún momento de este camino compartí laboratorio. A mis “amig@s de la universidad”, Ángel, Chules, Juan, Mar, Tere, Pepe… Gracias por las horas compartidas dentro y fuera de la facultad, porque este camino ha sido más llevadero a vuestro lado. Mucho ánimo a los que os queda ya poco para completar también este viaje. A aquellas personas que también llevo muy dentro en el corazón, y que siempre han tenido para mí una palabra de ánimo durante este tiempo, en especial a Rosario y Consue. La vida sólo tiene sentido cuando se comparte con personas maravillosas y os aseguro que para mí vosotr@s lo sois. A mis padres los cuales se merecen una mención más que especial, porque muchos de los logros de mi vida los he podido realizar gracias a ellos, sobretodo este. Os dedico este trabajo porque es el resultado del valor de la perseverancia que me habéis inculcado cada momento de mi vida. Gracias porque yo soy el fruto del enorme esfuerzo que habéis hecho a lo largo de vuestra vida. A mi hermana Cristina, porque tu llegada a mi mundo supuso sentar las bases de la responsabilidad en mi vida para intentar ser un buen ejemplo para ti, no se si lo he logrado pero como consecuencia de ello he llegado hasta aquí, por eso este trabajo es también parte de ti. Al resto de mi familia, y en especial a mis abuelos. Probablemente no entenderéis nada de lo que pone aquí, pero se que estáis orgullosos de vuestra nieta mayor, y con eso me sobra, por eso este éxito también es vuestro. A Cayetano, porque has sobrepasado todas las expectativas que deposité en tu persona, porque sin necesidad de pedirte nada, lo das todo; porque espero que sigas apoyándome incondicionalmente aunque no estés de acuerdo con mis decisiones, porque una parte de este triunfo también te pertenece. Y a aquellas personas que tal vez lean estas líneas y se sientan injustamente olvidadas, les pido de corazón que me perdonen, ya que es inevitable dejarse a alguien en el tintero. Por último, y en un alarde de narcisismo intencionado, me dedico esta tesis a mi misma porque después de todos estos años he conseguido culminar mi sueño. A mis padres y a mi hermana, Para ti, Caye, “Los sueños parecen al principio imposibles, luego improbables, y luego, cuando nos comprometemos, se vuelven inevitables” Mahatma Gandhi ABREVIATURAS AADH: amina aromática HPLC: cromatografía líquida de alta deshidrogenasa resolución AMP: adenosin monofosfato IPTG: isopropil-beta-D- Amp: ampicilina tiogalactopiranósido AP: presión atmosférica IS: secuencia de inserción ATCC: Colección Americana de Cultivos JGI: Joint Genome Institute (USA) Tipo Kb: kilobases de DNA o 1000 pb BLAST: Basic Local Alignment Search kDa: kilodalton Tool Km: kanamicina BSA: albúmina de suero bovino LAO: L-aminoácido oxidasa CECT: Colección Española de Cultivos LOD: actividad lisina oxidasa Tipo LodA: lisina-épsilon-oxidasa CIB: Centro de Investigaciones LTQ: lisina tirosilquinona Biológicas m/v: relación masa/volumen Cm: cloranfenicol m/z: relación masa/carga COG: Clusters of Orthologous Groups MADH: metilamina deshidrogenasa CSIC: Consejo Superior de MALDI: ionización por desorción láser Investigaciones Científicas asistida por una matriz CTQ: cisteína triptofilquinona mau: operón de utilización de DAO: D-aminoácido oxidasa metilamina DBM: motivo de unión a dinucleótidos MBP: proteína de unión a maltosa DMSO: dimetil sulfóxido MCO: multicobreoxidasa DNA: ácido desoxirribonucleico MCS: sitio de clonación múltiple DO: dopa oxidasa ML: Máxima verosimilitud (Maximum DO600: densidad óptica medida a 600 likelihood) nm MLodA: LodA multimérica DOE: Department of Energy (USA) MM: masa molecular Dopa: 3,4-dihidroxifenilalanina mob: región de movilización por DTT: ditiotreitol conjugación del plásmido RP4. EC: Comisión de Enzimas MP: Máxima parsimonia (Maximum EDTA: etilen diamino tetracetato (sal parsimony) disódica) MS/MS: espectrometría de masas en ESI: ionización por electrospray tándem FAD: flavín dinucleótido MS: espectrometría de masas FMN: flavín mononucleótido NADH: nicotinamida adenina Gm: gentamicina dinucleótido GoxA: glicina oxidasa NBT: azul de nitrotetrazolio Ha: hemaglutinina iii Ni-NTA agarosa: agarosa con níquel reb: genes que codifican los cuerpos R unido a nitrilotriacetato Rif: rifampicina NJ: Vecino más cercano (Neighbor- ROS: especies reactivas de oxígeno joining) rpm: revoluciones por minuto ori: origen de replicación SAM: S-adenosil metionina P: promotor SDS: dodecil sulfato sódico PAGE: electroforesis en gel de TEMED: N,N,N’,N’- poliacrilamida tetrametiletilendiamina pb: pares de bases en una secuencia de TFA: ácido trifluoroacético DNA. TH: actividad tirosina hidroxilasa PCR: reacción en cadena de la tnp: gen que codifica una transposasa polimerasa TOF: analizador de tiempo de vuelo PDB: Protein Data Base TPQ: topaquinona pH: concentración de protones Tris: Tris(hidroximetil)aminometano PHB: poli-β-hidroxibutirato TTQ: triptófano triptofilquinona Poli-His: etiqueta de poli-histidinas URF: unidades relativas de ppm: partes por millón fluorescencia PPO: actividad polifenol oxidasa UV: ultravioleta Ppo: enzima polifenol oxidasa V: voltio PQQ: pirroloquinolin quinona Δ: deleción (en la descripción de QHNDH: quinohemoproteína genotipos) deshidrogenasa iv ÍNDICES ÍNDICE DE CONTENIDOS ÍNDICES .................................................................................................................... V I. INTRODUCCIÓN ..................................................................................................... 1 I.1. CARACTERÍSTICAS GENERALES DE Marinomonas mediterranea. ........................... 3 I.1.1. El género Marinomonas. ............................................................................. 3 I.1.2. La bacteria marina M. mediterranea. .......................................................... 4 I.1.2.1. Características generales. ............................................................. 4 I.1.3. Polifenol oxidasas en M. mediterranea. ...................................................... 6 I.1.4. L-aminoácido oxidasas (LAOs). .................................................................... 8 I.1.4.1. LodA, L-lisina-épsilon-oxidasa de M. mediterranea. .................. 11 I.1.4.2. GoxA, glicina oxidasa de M. mediterranea. ...............................
Load more

Recommended publications
  • Comparative Proteomic Profiling of Newly Acquired, Virulent And
    www.nature.com/scientificreports OPEN Comparative proteomic profling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease Kerrie Ní Dhufaigh1*, Eugene Dillon2, Natasha Botwright3, Anita Talbot1, Ian O’Connor1, Eugene MacCarthy1 & Orla Slattery4 The causative agent of amoebic gill disease, Neoparamoeba perurans is reported to lose virulence during prolonged in vitro maintenance. In this study, the impact of prolonged culture on N. perurans virulence and its proteome was investigated. Two isolates, attenuated and virulent, had their virulence assessed in an experimental trial using Atlantic salmon smolts and their bacterial community composition was evaluated by 16S rRNA Illumina MiSeq sequencing. Soluble proteins were isolated from three isolates: a newly acquired, virulent and attenuated N. perurans culture. Proteins were analysed using two-dimensional electrophoresis coupled with liquid chromatography tandem mass spectrometry (LC–MS/MS). The challenge trial using naïve smolts confrmed a loss in virulence in the attenuated N. perurans culture. A greater diversity of bacterial communities was found in the microbiome of the virulent isolate in contrast to a reduction in microbial community richness in the attenuated microbiome. A collated proteome database of N. perurans, Amoebozoa and four bacterial genera resulted in 24 proteins diferentially expressed between the three cultures. The present LC–MS/ MS results indicate protein synthesis, oxidative stress and immunomodulation are upregulated in a newly acquired N. perurans culture and future studies may exploit these protein identifcations for therapeutic purposes in infected farmed fsh. Neoparamoeba perurans is an ectoparasitic protozoan responsible for the hyperplastic gill infection of marine cultured fnfsh referred to as amoebic gill disease (AGD)1.
    [Show full text]
  • Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
    Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341
    [Show full text]
  • 87-Fernanda-Cid.Pdf
    UNIVERSIDAD DE LA FRONTERA Facultad de Ingeniería y Ciencias Doctorado en Ciencias de Recursos Naturales Characterization of bacterial communities from Deschampsia antarctica phyllosphere and genome sequencing of culturable bacteria with ice recrystallization inhibition activity DOCTORAL THESIS IN FULFILLMENT OF THE REQUERIMENTS FOR THE DEGREE DOCTOR OF SCIENCES IN NATURAL RESOURCES FERNANDA DEL PILAR CID ALDA TEMUCO-CHILE 2018 “Characterization of bacterial communities from Deschampsia antarctica phyllosphere and genome sequencing of culturable bacteria with ice recrystallization inhibition activity” Esta tesis fue realizada bajo la supervisión del Director de tesis, Dr. Milko A. Jorquera Tapia, profesor asociado del Departamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera y co-dirigida por el Dr. León A. Bravo Ramírez, profesor titular del Departamento de Ciencias Agronómicas y Recursos Naturales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera. Esta tesis ha sido además aprobada por la comisión examinadora. Fernanda del Pilar Cid Alda …………………………………………… ……………………………………. Dr. MILKO JORQUERA T. Dr. Francisco Matus DIRECTOR DEL PROGRAMA DE …………………………………………. DOCTORADO EN CIENCIAS DE Dr. LEON BRAVO R. RECURSOS NATURALES …………………………………………. Dra. MARYSOL ALVEAR Z. ............................................................ Dr. Juan Carlos Parra ………………………………………… DIRECTOR DE POSTGRADO Dra. PAULINA BULL S. UNIVERSIDAD DE LA FRONTERA ………………………………………… Dr. LUIS COLLADO G. ………………………………………… Dra. ANA MUTIS T Dedico esta tesis a mis padres, Lillie Alda Leiva y Fernando Cid Verdugo quienes dedicaron sus vidas a la tarea de enseñar en la Universidad de La Frontera, Temuco-Chile. Abril, 2018 Agradecimientos /Acknowledgements Agradecimientos /Acknowledgements This study was supported by the following research projects: Doctoral Scholarship from Conicyt (no. 21140534) and La Frontera University.
    [Show full text]
  • Degrading Bacteria in Deep‐
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Aberdeen University Research Archive Journal of Applied Microbiology ISSN 1364-5072 ORIGINAL ARTICLE Hydrocarbon-degrading bacteria in deep-water subarctic sediments (Faroe-Shetland Channel) E. Gontikaki1 , L.D. Potts1, J.A. Anderson2 and U. Witte1 1 School of Biological Sciences, University of Aberdeen, Aberdeen, UK 2 Surface Chemistry and Catalysis Group, Materials and Chemical Engineering, School of Engineering, University of Aberdeen, Aberdeen, UK Keywords Abstract clone libraries, Faroe-Shetland Channel, hydrocarbon degradation, isolates, marine Aims: The aim of this study was the baseline description of oil-degrading bacteria, oil spill, Oleispira, sediment. sediment bacteria along a depth transect in the Faroe-Shetland Channel (FSC) and the identification of biomarker taxa for the detection of oil contamination Correspondence in FSC sediments. Evangelia Gontikaki and Ursula Witte, School Methods and Results: Oil-degrading sediment bacteria from 135, 500 and of Biological Sciences, University of Aberdeen, 1000 m were enriched in cultures with crude oil as the sole carbon source (at Aberdeen, UK. ° E-mails: [email protected] and 12, 5 and 0 C respectively). The enriched communities were studied using [email protected] culture-dependent and culture-independent (clone libraries) techniques. Isolated bacterial strains were tested for hydrocarbon degradation capability. 2017/2412: received 8 December 2017, Bacterial isolates included well-known oil-degrading taxa and several that are revised 16 May 2018 and accepted 18 June reported in that capacity for the first time (Sulfitobacter, Ahrensia, Belliella, 2018 Chryseobacterium). The orders Oceanospirillales and Alteromonadales dominated clone libraries in all stations but significant differences occurred at doi:10.1111/jam.14030 genus level particularly between the shallow and the deep, cold-water stations.
    [Show full text]
  • Marinomonas Brasiliensis Sp
    IJSEM Papers in Press. Published June 18, 2010 as doi:10.1099/ijs.0.024661-0 1 Marinomonas brasilensis sp. nov. isolated from the coral Mussismilia 2 hispida and reclassification of Marinomonas basaltis as a later synonym 3 of Marinomonas communis 4 5 Luciane A. Chimetto1,2,3, Ilse Cleenwerck3, Marcelo Brocchi1, Anne Willems4, 6 Paul De Vos3,4, and Fabiano L. Thompson2* 7 8 1Departament of Genetics, Evolution and Bioagents, Institute of Biology, State University of 9 Campinas (UNICAMP), Brazil 10 11 2Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), 12 Brazil 13 14 3BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, 15 Belgium 16 17 4Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B- 18 9000 Ghent, Belgium 19 20 *Corresponding author: Fabiano L. Thompson. Phone/FAX: +55-21-25626567. 21 E-mail: [email protected] 22 23 Running Title: 24 Marinomonas brasilensis sp. nov. 25 Subject Category: New taxa, Proteobacteria 26 27 Footnote: The GenBank/EMBL accession number for the 16S rRNA gene 28 sequence of strain R-40503T is GU929940. 29 30 31 32 33 34 35 36 37 38 39 40 1 41 Abstract 42 A Gram-negative, aerobic bacterium, designated R-40503T was isolated from 43 mucus of the reef builder coral, Mussismilia hispida, located in the São 44 Sebastião Channel, São Paulo, Brazil. Phylogenetic analyses revealed that 45 strain R-40503T belongs to the genus Marinomonas. The 16S rRNA gene 46 sequence similarity of R-40503T was above 97 % with the type strains of 47 Marinomonas vaga, M.
    [Show full text]
  • Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica
    Mar. Drugs 2011, 9, 184-195; doi:10.3390/md9020184 OPEN ACCESS Marine Drugs ISSN 1660-3397 www.mdpi.com/journal/marinedrugs Article Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica Yong Yu *, Hui-Rong Li, Yin-Xin Zeng and Bo Chen SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai, 200136, China; E-Mails: [email protected] (H.-R.L.); [email protected] (Y.-X.Z.); [email protected] (B.C.) * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +86-21-58717207; Fax: +86-21-58711663. Received: 17 December 2010; in revised form: 4 January 2011 / Accepted: 14 January 2011 / Published: 31 January 2011 Abstract: The diversity and cold-active hydrolytic enzymes of culturable bacteria associated with sandy sediment from Nella Fjord, Eastern Antarctica (69°22′6″ S, 76°21′45″ E) was investigated. A total of 33 aerobic heterotrophic bacterial strains were isolated at 4 °C. These bacterial isolates could be sorted into 18 phylotypes based on the 16S rRNA gene sequence belonging to four phyla, namely Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria. Only seven isolates were psychrophilic, 15 isolates were moderately psychrophilic, and 11 isolates were psychrotolerant. More than 72% of the isolates required sodium chloride to grow. Esterase, -glucosidase and proteases activities at 4 °C were detected in more than 45% of the strains while approximately 21%, 15% and 12% of the strains possessed lipase, amylase and chitinase, respectively. These results indicate that a relatively high culturable bacterial diversity is present within marine sediment of Nella Fjord and it could serve as an ideal candidate region for bioprospecting.
    [Show full text]
  • Marinomonas Basaltis Sp. Nov., a Marine Bacterium Isolated from Black Sand
    International Journal of Systematic and Evolutionary Microbiology (2008), 58, 2743–2747 DOI 10.1099/ijs.0.65724-0 Marinomonas basaltis sp. nov., a marine bacterium isolated from black sand Ho-Won Chang,1 Seong Woon Roh,1,2 Kyoung-Ho Kim,1 Young-Do Nam,1,2 Jung-Hoon Yoon,1 Hee-Mock Oh1 and Jin-Woo Bae1,2,3 Correspondence 1Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea Jin-Woo Bae 2Korea University of Science and Technology, 52, Eoeun-dong, Daejeon 305-333, Republic of [email protected] Korea 3Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea A Gram-negative, aerobic, slightly halophilic, rod-shaped bacterium was isolated from black sand in Soesoggak, Jeju island, Korea. The strain, designated J63T, was oxidase- and catalase-positive + and arginine dihydrolase-negative. The isolate required Na for growth and differed from phenotypically related species by being able to utilize sucrose and D-galactose as a carbon source. Phylogenetic analysis based on the sequence of the 16S rRNA gene revealed that strain J63T belongs to the genus Marinomonas. It exhibited 16S rRNA gene sequence similarities of 97.6–98.7 % to the closely related species Marinomonas communis, Marinomonas ostreistagni, Marinomonas aquimarina and Marinomonas vaga. The phylogenetic analysis revealed that strain J63T comprised a relatively long subline of descent, shared a branch point with the outlying species Marinomonas communis and occupied a phylogenetically distant position on the main Marinomonas branch. Based on DNA–DNA hybridization, the levels of relatedness between strain J63T and M.
    [Show full text]
  • “Investigaç Compostos a “Genetic an of Antimi
    PAULA DE FRANÇA “INVESTIGAÇÃO GENÉTICA E FUNCIONAL DA PRODUÇÃO DE COMPOSTOS ANTIMICROBIANOS POR BACTÉRIAS ORIUNDAS DA ANTÁRTICA” “GENETIC AND FUNCTIONAL EVALUATION OF PRODUCTION OF ANTIMICROBIAL COMPOUNDS BY BACTERIA FROM ANTARCTICA” CAMPINAS 2015 i ii UNIVERSIDADE ESTADUAL DE CAMPINAS Instituto de Biologia PAULA DE FRANÇA “INVESTIGAÇÃO GENÉTICA E FUNCIONAL DA PRODUÇÃO DE COMPOSTOS ANTIMICROBIANOS POR BACTÉRIAS ORIUNDAS DA ANTÁRTICA” Orientadora: Profª. Drª. Fabiana Fantinatt i-Garboggini Co-orientadora: Profª. Drª. Marta Cristina Teixeira Duarte “GENETIC AND FUNCTIONAL EVALUATION OF PRODUCTION OF ANTIMICROBIAL COMPOUNDS BY BACTERIA FROM ANTARCTICA” Dissertação apresentada ao Instituto de Biologia da Universidade Estadual de Campinas como parte dos requisitos exigidos para obtenção do título de Mestra em Genética e Biologia Molecular, na área de Microbiologia. Dissertation presented to the Biology Institute of the University of Campinas in partial fulfill of the requirements fo r the Degree of Master in Genetics and Molecular Biology, Microbiology area. ESTE EXEMPLAR CORRESPONDE À VERSÃO FINAL DA DISSERTAÇÃO DEFENDIDA PELA ALUNA PAULA DE FRANÇA E ORIENTADA PELA PROFª. DRª. FABIANA FANTINATTI -GARBOGGINI CAMPINAS 2015 iii Ficha catalográfica Universidade Estadual de Campinas Biblioteca do Instituto de Biologia Mara Janaina de Oliveira - CRB 8/6972 França, Paula, 1987- F844i Fra Investigação genética e funcional da produção de compostos antimicrobianos por bactérias oriundas da Antártica / Paula de França. – Campinas, SP : [s.n.], 2015. Fra Orientador: Fabiana Fantinatti Garboggini. Fra Coorientador: Marta Cristina Teixeira Duarte. Fra Dissertação (mestrado) – Universidade Estadual de Campinas, Instituto de Biologia. Fra 1. Bactérias. 2. Atividade antimicrobiana. 3. Concentração inibitória mínima. 4. 16S rRNA. 5. Policetídeo sintases. 6. Peptídeo sintases. I. Fantinatti- Garboggini, Fabiana.
    [Show full text]
  • Alcanivorax Strain Detected Among the Cultured Bacterial Community from Sediments Affected by the ‘Prestige’ Oil Spill
    Vol. 362: 25–36, 2008 MARINE ECOLOGY PROGRESS SERIES Published June 30 doi: 10.3354/meps07431 Mar Ecol Prog Ser Alcanivorax strain detected among the cultured bacterial community from sediments affected by the ‘Prestige’ oil spill Jorge Alonso-Gutiérrez1, Maria M. Costa1, Antonio Figueras1, Joan Albaigés2, Marc Viñas3, 4, Anna M. Solanas3, Beatriz Novoa1,* 1Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello 6, Vigo 36208, Spain 2IIQAB-CSIC, Jordi Girona 18–26, 08034 Barcelona, Spain 3Department of Microbiology, University of Barcelona, Diagonal 645, 08028 Barcelona, Spain 4GIRO Technological Centre, Rambla Pompeu Fabra, 1, 08100 Mollet del Vallès, Spain ABSTRACT: Sediments sampled next to the Cíes Islands, a natural reserve of the National Park of Atlantic Islands in Ría de Vigo (Spain), 3 mo after the first tide of fuel from the ‘Prestige’ tanker arrived, were chemically and microbiologically characterized. The chemical analysis of the sedi- ments, using oil fingerprinting techniques, showed the occurrence of fresh ‘Prestige’ fuel oil with a background of older hydrocarbon contamination. The cultured bacterial community of the contami- nated sediments harbored a high population of total heterotrophs and alkane degraders and a small proportion of aromatic-degrading bacteria. Based on partial 16S ribosomal RNA gene sequence data, 37 different bacterial strains, isolated in diluted marine agar, were detected. Most of them were clas- sified as members of the groups Gammaproteobacteria (59%) and Alphaproteobacteria (21%), although members of the Bacteroidetes (10%) and of Firmicutes (10%) were also found. Some of the different identified bacteria have previously been described as fuel oil–degrading species such as Alcanivorax, Shewanella, Vibrio, Pseudoalteromonas and Marinomonas.
    [Show full text]
  • Microorganisms Associated with Ulva Grown in Abalone Effluent Water: Implications for Biosecurity
    MICROORGANISMS ASSOCIATED WITH ULVA GROWN IN ABALONE EFFLUENT WATER: IMPLICATIONS FOR BIOSECURITY DJGKRI001 KRISTIN DE JAGER Dissertation presented in the partial fulfilment of the requirements for the degree of Master of Science (BIO 5006W Applied Marine Science) Department of Biological Science University of Cape Town February 2019 Supervisor: Prof John J. Bolton (University of Cape Town) Co-supervisor: Dr Brett M. Macey University(Department of Agriculture, of Forestry Cape and Fisheries) Town The copyright of this thesis vests in the author. No quotation from it or information derived from it is to be published without full acknowledgement of the source. The thesis is to be used for private study or non- commercial research purposes only. Published by the University of Cape Town (UCT) in terms of the non-exclusive license granted to UCT by the author. University of Cape Town P a g e | 1 DECLARATION I know the meaning of plagiarism and declare that all of the work in the dissertation (or thesis), other than that which was carried out by the University of the Western Cape Next Generation Sequencing Facility and that which is properly acknowledged, is my own work as assisted by my supervisors. This thesis has not been submitted in this or any form to another university. Experimental work discussed in this thesis was carried out under the supervision of Prof. J. J. Bolton of the Department of Botany, University of Cape Town and Dr. B.M. Macey of the Department of Agriculture, Forestry and Fisheries. Mr Kristin de Jager Date: P a g e | 2 DEDICATIONS I dedicate this thesis to all things green, as without words they strive to answer questions we have never asked.
    [Show full text]
  • Marinomonas Mediterranea Perteneciente a Una Nueva Familia De Quinoproteínas
    UNIVERSIDAD DE MURCIA FACULTAD DE BIOLOGÍA Identificación y Caracterización de una Glicina Oxidasa en Marinomonas mediterranea Perteneciente a una Nueva Familia de Quinoproteínas D. Jonatan Cristian Campillo Brocal 2016 UNIVERSIDAD DE MURCIA FACULTAD DE BIOLOGÍA Identificación y caracterización de una glicina oxidasa en Marinomonas mediterranea perteneciente a una nueva familia de quinoproteínas D. Jonatan Cristian Campillo Brocal 2016 El presente trabajo ha sido entregado para optar al grado de doctor en "Biología Molecular y Biotecnología". Asimismo ha sido propuesto para Mención de Doctorado Internacional en virtud a la estancia predoctoral realizada en el “Laboratorio de Biodiversidad Microbiana y Biotecnológica” (LBBM) situado en el Observatorio Oceanográfico de Banyuls sur Mer (Francia). Esta Tesis Doctoral ha sido desarrollada durante el periodo de disfrute de una beca-contrato predoctoral de la Universidad de Murcia y ha sido financiada por el Ministerio de Ciencia e Innovación (Proyecto BIO2010-15226) y por la Fundación Séneca de la Región de Murcia (Proyecto 11867/PI/09). El trabajo realizado en la presente memoria ha dado lugar a las siguientes publicaciones: 1. Campillo-Brocal, J. C., P. Lucas-Elío and A. Sánchez-Amat. (2013) Identification in Marinomonas mediterranea of a novel quinoprotein with glycine oxidase activity. MicrobiologyOpen. 2 (4): 684-694. doi: 10.1002/mbo3.107. 2. Chacón-Verdú, M. D., J. C. Campillo-Brocal, P. Lucas-Elío, V. L. Davidson and A. Sánchez-Amat. (2015) Characterization of recombinant biosynthetic precursors of the cysteine tryptophylquinone cofactors of L-lysine-epsilon-oxidase and glycine oxidase from Marinomonas mediterranea. BBA-Proteins and Proteomics. 1854 (9): 1123–1131. doi: 10.1016/j.bbapap.2014.12.018.
    [Show full text]
  • (Marine Microbiology) Programme Approved by the Board of Studies in Microbiology on 23Rd April 2012
    Goa University P.O. Goa University, Taleigao Plateau, Goa 403 206, India Syllabus of M.Sc. (Marine Microbiology) Programme Approved by the Board of Studies in Microbiology on 23rd April 2012. The department of Microbiology offers a two-year full time M.Sc. Marine Microbiology programme w.e.f. June 2012. This is an Innovative programme of teaching and research in interdisciplinary and emerging areas sponsored by UGC. A brief description of the course: Purpose: The course is designed to develop human resource in the field of Marine Microbiology at the level where the candidates will be equipped to take up research programmes and jobs in the related Industries sector, Institutions and Academics. The main objective of this Programme is to train manpower in the field of Marine Microbiology. Prerequisites: B. Sc (Microbiology) Credits: The Programme consists of Courses with a total of 80 credits – Theory: 60 credits, Practicals: 20 credits Number of Semesters, Course distribution: The Programme comprises 4 Semesters; each Semester has Courses equivalent of 20 credits Dissertation: Compulsory, with 12 credits, in Semester III & IV, to impart training in research Field work/Case studies/Cruise/Field trip/Report writing/Training in other University/Institute: to impart specialized practical training in specific areas of Marine Microbiology. 1 M. Sc Marine Microbiology: Course structure In the following tables L refers to Lectures, T to Tutorials and P to Practicals. Description of course appears on the page numbers listed in the tables.
    [Show full text]