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Brochure/Abstract Book nd 2 Annual European MicroCal Meeting Paris, September 26-27, 2016 Welcome to the 2nd Annual European MicroCal Meeting On behalf of Malvern Instruments, Institut Pasteur, the ARBRE-MOBIEU COST Action and the organizing committee, it is our pleasure to welcome you to MicroCal European Users’Meeting 2016 in Paris. We would like to thank all our attendees and speakers. The organization of this meeting would not have been possible without their support. We do hope that you will enjoy intense and stimulating discussions as well as an unforgettable stay in the light city Paris... Introduction The second Annual European MicroCal Meeting, co-organized by Malvern Instruments, Institut Pasteur and ARBRE European molecular biophysics network, will focus on applications, best practices, advanced data analysis and latest developments in isothermal titration and differential scanning calorimetry (ITC and DSC) and will bring together MicroCal users from across Europe. The 2-days event will include ITC and DSC workshops and scientific sessions with panel discussions focused on the data quality, best experimental practices, new applications and advanced data analysis. It allows users to share and broaden their experiences and network on these technologies during application-specific oral presentations, poster sessions, panel discussions and workshops. Attend seminars and roundtables with European microcalorimetry experts. Meet MicroCal application scientists and discover several microcalorimetric systems on display. Location Institut Pasteur Paris, 28 rue du Docteur Roux, 75015 Paris http://www.pasteur.fr/en/adress-and-access Venue By Metro or Bus: By Metro: Get off at either Volontaires (line 12) or Pasteur (lines 6 and 12) station. These stations are just a 5 minute walk from the Institut Pasteur. Map of the metro network: http://www.ratp.fr/en/ratp/c_5000/accueil By Bus: Bus number 95 (Institut Pasteur stop, direction Porte de Vanves) stops in front of the Institute’s entrance. Bus numbers 39 and 70 (Volontaires-Vaugirard or Sèvres-Lecourbe stops) stop slightly further away (5 to 10 minute walk) and not far from Pasteur metro station. Map of bus routes: http://www.ratp.fr/en/ratp/c_5000/accueil Note: One ticket allows you to travel on the metro and includes all connections as long as you do not leave the metro network. For most bus and tram routes in Paris and Ile-de-France, one ticket is valid for a single journey without connections regardless of length. On some routes (Balabus, Noctambus, Orlybus, Roissybus, 221, 297, 299, 350 and 351) fares depend on journey length. By car: Rue du Docteur Roux is a one way street and you pay for parking spaces. Paying public car parks are available at the following addresses: Sogeparc France Car park 81 r Falguière - 75015 Paris 50 av Maine - 75015 Paris Tel.: +33 (0)1 43 35 27 69 Direct access to Montparnasse station and TGV Tel.: +33 (0)1 43 21 50 30 From Paris airports: The journey from Paris airports to the Institut Pasteur takes from 45 minutes (if you are coming from Orly airport) to 90 minutes (if you are coming from Roissy-Charles de Gaulle airport) Orly airport : Tel.: +33 (0)1 49 75 15 15 Different routes are possible from Orly airport: By Orlyval, you must buy a special ticket to travel between Paris and Orly via Antony which is valid throughout the metro and RER networks. At the Antony RER station, take RER line B to Denfert-Rochereau station. Then take line 6 to Pasteur station. By Orlybus, you must also buy a special ticket to travel by bus between Paris (Terminus at Denfert-Rochereau) and Orly. From Denfert-Rochereau take the metro (line 6 direction Charles de Gaulle - Etoile) to Pasteur station. You can take the Air France bus to Paris-Montparnasse. You can either walk about 15 minutes or take the metro (line number 6 direction Charles de Gaulle - Etoile) to get from Montparnasse-Bienvenue station to Pasteur station. An Orlyrail shuttle will also take you to the RER C (Aéroport d’Orly station) Finally, the Jetbus will take you to the Villejuif-Aragon metro station, terminus of line 7. Roissy - Charles de Gaulle airport : Tel.: +33 (0)1 48 62 22 80 Different routes are possible from Roissy – Charles de Gaulle airport: Cars Air France (01 41 56 89 00) : CdG - Porte Maillot - Arc de Triomphe CdG - Gare de Lyon - Gare Montparnasse Metro/RER: Go to the RER station at the airport accessible from terminals 1 or 2, or use the free Aéroports de Paris shuttles if your flight lands at the other terminals. Take the RER B to Denfert-Rochereau. Then take line 6 to Pasteur station. By Roissybus This bus goes from the airport to rue Scribe, near place de l’Opéra. Tickets are on sale at line terminus, in the airports and on board buses. By train, from Paris' main SNCF stations: Paris has several SNCF (railway) stations. Below are the routes by metro between the Institut Pasteur and the main stations. “Montparnasse” station This is the closest station to the Institut Pasteur. You can either walk about 15 minutes or take line 6 to Pasteur station. “Saint Lazare” station Take line 12 to Pasteur station (about 20 minutes). “Gare de Lyon” station Take line 14 to Bercy station. Change to line 6 to Pasteur station. Journey time is roughly 35 minutes. “Austerlitz” station Take line 5 to Place d’Italie. Change to line 6 to Pasteur station. Journey time is roughly 30 minutes. “Gare de l’Est” station Take line 4 to Raspail station. Then change to line 6 to Pasteur station. Journey time is roughly 35 minutes. “Gare du Nord” station Take the RER B to Denfert-Rochereau. Change and take line 6 to Pasteur station. Journey time is roughly 35 minutes. Campus map Agenda Monday, September 26th (Amphithéâtre Duclaux) Meeting Opens 08:00-08:45 Registration and poster installation 08:45-09:00 Opening remarks by the host and chair of ARBRE MOBIEU, Patrick England, Institut Pasteur, Paris, France Morning Session 1 Interaction analysis: The value of orthogonal approaches Chair: Patrick England, Institut Pasteur, Paris, France 09:00-09:30 "The Power of Orthogonal Biophysical Methods Exemplified by An Integrative Structural Biology Study of FGFR1" Geoff Holdgate, AstraZeneca, Cambridge, UK 09:30-09:50 "Combining isothermal titration calorimetry, fluorescence anisotropy and kinetic approaches to decipher the molecular mechanisms of the redox activation of the Yap1 transcription factor in S. cerevisiae" Sophie Rahuel-Clermont, Université de Lorraine, Nancy, France 09:50-10:10 "What have we learned from the enthalpy database of 200 compound binding to 8 carbonic anhydrase isoforms as determined by isothermal titration calorimetry" Daumantas Matulis, Vilnius University, Lithuania 10:10-10:30 Flash presentation of posters (1 slide & 2min/talk) 9 speakers : Regina Adao, University of Campinas, Sao Paulo, Brazil Mathieu Coinçon, Stockholm University, Sweden Andrea Gohlke, Cancer Research Institute UK Beatson Institute, Glasgow, UK Sergei Kuprin, Swedish Orphan Biovitrum AB, Stockholm, Sweden Vaida Linkuviene, Vilnius University, Lithuania Anna Mårtensson, Chalmers University of Technology, Gothenburg, Sweden Ksenia Maximova , Warsaw University, Poland Serena Rinaldo, Istituto Pasteur-Fondazione Cenci Bolognetti, Rome, Italy Katharina Weickhmann, Goethe University, Frankfurt, Germany 10:30-11:00 Coffee break and 1st poster session Morning Session 2 Focus on DSC: Differential Scanning Calorimetry Chair: Michaela Wimmerova, Brno, Czech Republic 11:00-11:30 "Thermal dissociation and unfolding of insulin and effects of ligand interactions studied by DSC. Predictability of insulin analogue stability using DSC and correlation with other methods" Kasper Huus, Novo Nordisk, Denmark 11:30-11:50 "Study of rabies virus by Differential Scanning Calorimetry: Identification of Proteins Involved in Thermal Transitions" Frederic Greco, Sanofi Pasteur, Marcy l'Etoile, France 11:50-12:10 "Using Differential Scanning Calorimetry to monitor glioblastoma evolution" François Devred, Université Aix-Marseille, France 12:10-12:30 "DSC in application to the studies of blood serum samples" Adrian Velazquez Campoy, Zaragoza University, Spain 12:30-13:30 Lunch 13:30-14:15 Panel discussion 1 "Best practices in DSC experimental setup and data analysis" Chaired by Margarida Bastos, Porto University, Portugal and Natalia Markova, Malvern Instruments, UK Afternoon session 1 Focus on ITC: Isothermal Titration Calorimetry Chair: Chris Johnson, Cambridge, UK 14:15-14:35 "Study of peptide/lipid interactions by ITC and DSC" Isabel Alves, CBMN, Bordeaux, France 14:35-14:55 "Enthalpic Forces Correlate with the Selectivity of Transthyretin-Stabilizing Ligands in Human Plasma" Kristoffer Brännström, Umea University, Sweden 14.55-15:15 "Affinity based definition of RNA motifs and strategic protein-RNA mutational design" Cameron Mackereth, IECB, Bordeaux, France 15:15-15:45 Coffee break and 2nd poster session Afternoon session 2 Novel developments in the field of calorimetry Chair: Margarida Bastos, Porto, Portugal 15:45-16:05 "The underestimated potential of isothermal microcalorimetry for microbiological studies" Olivier Braissant , Basel University, Switzerland 16:05:16:25 "ITC studies of enzyme activity in solution and suspensions" Peter Westh, Roskilde University, Denmark 16:25-16:45 "Use of isothermal titration calorimetry to investigate and identify novel peptide substrates for prolyl carboxypeptidase. A technology comparison" Anne-Marie Lambeir, Antwerp University, Belgium 16:45-17:05 "KinITC – Kinetic Revolution in Drug Discovery" Pascal Zihlmann, Basel University,
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