Abstract Supplement

2018 ABSTRACT SUPPLEMENT SAN FRANCISCO June 20-23 • Marriott Marquis Federation of Clinical Immunology Societies June 20-23, 2018 San Francisco, California FOCIS 2018 Abstract Supplement TABLE OF CONTENTS Abstracts by Subject Area …………………………………………………………...……………………………….2 Allergy/asthma ……………………………………………………………………………………………………...2 Autoimmune neurologic diseases .............................................................................................................. 7 Autoimmune rheumatologic diseases ...................................................................................................... 19 Bone marrow or stem cell transplantation ............................................................................................... 37 Cytokines/chemokines ............................................................................................................................ 42 Diabetes and other autoimmune endocrine diseases .............................................................................. 47 General Autoimmunity…………………………………………………………………………………………….58 Genetics .................................................................................................................................................. 70 Immune monitoring .................................................................................................................................. 74 Immunity & infection ................................................................................................................................ 82 Immunodeficiency: primary or acquired ................................................................................................. 103 Immuno-dermatology ............................................................................................................................ 111 Immunology of the eye .......................................................................................................................... 115 Immuno-oncology .................................................................................................................................. 118 Inflammatory bowel diseases ................................................................................................................ 134 Innate immunity ..................................................................................................................................... 139 Organ transplantation ............................................................................................................................ 150 Other ..................................................................................................................................................... 154 Reproductive immunology ..................................................................................................................... 155 Therapeutics/pharmacology .................................................................................................................. 159 Transplantation...................................................................................................................................... 168 Abstract Index by Subject Area ................................................................................................................. 176 Abstract Index by FOCIS Pillar .................................................................................................................. 177 Abstract Index by Author …………………………………………………………………………………………..178 1 Allergy/asthma F.35. Physicochemical Characterization of Philippine Grass Flora for Diagnosis of Respiratory Allergies Leonora Autus-Geniston1, Mary Anne Castor2, Ronald Matias3 and Alexander Tuazon3 1St Paul University-Quezon City/United Bayanihan Foundation/United Lab. Inc., Quezon City, National Capital Region, Philippines, 2Philippine General Hospital, Manila, National Capital Region, Philippines, 3United Laboratories, Inc, Mandaluyong, National Capital Region, Philippines As a tropical country, grass pollen grains are the important causes of respiratory allergies in the Philippines. There are different flora in the Philippines compared to that of the western countries where the pollen standards in clinical practice are imported for diagnosis of respiratory allergies. The use of local pollen extracts are important for personalized treatment. Since these pollen extracts have not yet characteirzed, they have to be processed to improve the extracts'; quality. The grass pollen extracts from Cyanodon dactylon (bermuda), Axonopus compressus (carabao), Imperata cylindrica (cogon), and Saccharum spontaneum (talahib) were separated by gradient SDS-PAGE and immunoblotted against IgE. Bands were visualized with Fluorchem C2 aided with Alpha View software. The total protein extracts ranged from 281.3-986.6 ug/ml. The protein sizes ranged from 14.4-66.3 kDa: carabao grass, 3.5-66.3 kDa; cogon, 3.5-200 kDa; and talahib, 21.6-66.3 kDa. A single IgE-binding protein band at 55.4 kDa corresponding to known groups of grass pollen allergens was identified in the serum sample in the patient with respiratory allergies. The study showed the varying concentrations of grass pollen extracts as well as the pollen profile of their sizes and these correspond to known groups of grass pollen allergens, and found that the clinical diagnosis is consistent with the identification of the known allergen of the patient with the aid of the local protein extracts. Keywords: Philippines, Cyanodon dactylon (bermuda), Axonopus compressus (carabao), Imperata cylindrica (cogon), and Saccharum spontaneum (talahib), diagnostic protein extract, clinical practice F.53. IL-10 Producing Lung Resident Memory TR1-like Cells do not Protect Against Allergic Airway Inflammation Carlos Medina1, Koshika Yadava2, Irina Gurevich1, Heather Ishak2, Hedwich Kuipers2 and Paul Bollyky2 1Stanford University School of Medicine, Stanford, CA, 2Stanford University, Stanford, CA Recent studies have identified allergen-specific tissue resident memory T cells that mediate allergic airway inflammation. However it remains unknown whether an analogous regulatory population exists. Given that previous studies have demonstrated a clear association between IL-10 induction and the long-term efficacy of immunotherapy, we hypothesized that an analogous population of IL-10 producing tissue resident memory T cells could also be present in the lungs of previously sensitized mice. Using a house dust mite induced model of allergic airway inflammation we characterized the cellular sources and the temporal and spatial aspects of endogenous IL-10 production. We find that in the setting of allergic inflammation TR1-like CD4+ Foxp3- T cells are the main producers of IL-10. Notably, these cells accumulate in the lungs and persist long after the resolution of inflammation as part of the CD44hi CD62low resident memory T cell pool. However, these tissue-resident memory TR1 cells are not sufficient to prevent inflammation in response to allergen re-challenge. Local depletion in the lung had no effect on airway inflammation and adoptive transfer of these cells into naïve mice was insufficient to protect against allergen re-challenge. Together these data demonstrate that although endogenous TR1-like resident memory cells are induced upon allergen sensitization, they are insufficient to regulate inflammation upon subsequent allergen re-challenge. Future studies focused on increasing the local pool of TR1-like resident memory cells may provide a means of raising the threshold of allergic activation to protect against further allergic challenges. 2 F.99. Duration of attenuation of allergic responses after modulation of idiotypic regulatory network Reginald Gorczynski1, Cesar Francisco Lara Alvarez2, Edwin Gershon3, George Hoffmann3, Ernesto Morales4 and Geoffrey Hoffmann3 1Sunnybrook Health Science Centre, Toronto, ON, Canada, 2Biodextra, Mexico City, Distrito Federal, Mexico, 3Network Immunology, Vancouver, BC, Canada, 4BioDextra, Mexico City, Distrito Federal, Mexico We showed that co-injection of antigen-specific plus anti-idiotypic antibodies could suppress OVA-induced IgE production (JIMR: 2017). Antigen-specific antibodies were produced by conventional immunization of mice (using tetanus toxoid or skin allografting). Anti-idiotypic antibodies were derived by absorption of antigen-specific antibody. Both antibodies with complementary specificity are hypothesized to stimulate two populations of T lymphocytes. Prior to using this treatment approach in larger animals and humans, we have first assessed the longevity of the attenuation afforded by this approach. Mice received 5 weekly im injections of a mixture of the antibodies described above, and at the same time began immunization with OVA in alum (two injections 14d apart). Control animals received normal mouse Ig only along with OVA imunization. Following the second OVA injection all mice were maintained on exposure to allergen by drinking egg white solution with 1% glucose. 14 and 80 days after the final injections of the antibody mixture groups of mice received a final OVA immunization in alum and were sacrificed 10d later. Serum was collected for asays of OVA-specific IgG and IgE responses, and splenocytes stimulated in vitro with ConA or OVA to assess mitogen and OVA-induced L4 and Il-2 production. Attenuation of OVA-IgE serum levels, and of OVA-induced IL-4 production in vitro, was seen even in mice receiving a boost in OVA-immunization 80d after the last

Load more