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Chapter 24 Primary Sex Organs = Gonads Produce Gametes Secrete Hormones That Control Reproduction Secondary Sex Organs = Accessory Structures
Anatomy Lecture Notes Chapter 24 primary sex organs = gonads produce gametes secrete hormones that control reproduction secondary sex organs = accessory structures Development and Differentiation A. gonads develop from mesoderm starting at week 5 gonadal ridges medial to kidneys germ cells migrate to gonadal ridges from yolk sac at week 7, if an XY embryo secretes SRY protein, the gonadal ridges begin developing into testes with seminiferous tubules the testes secrete androgens, which cause the mesonephric ducts to develop the testes secrete a hormone that causes the paramesonephric ducts to regress by week 8, in any fetus (XX or XY), if SRY protein has not been produced, the gondal ridges begin to develop into ovaries with ovarian follicles the lack of androgens causes the paramesonephric ducts to develop and the mesonephric ducts to regress B. accessory organs develop from embryonic duct systems mesonephric ducts / Wolffian ducts eventually become male accessory organs: epididymis, ductus deferens, ejaculatory duct paramesonephric ducts / Mullerian ducts eventually become female accessory organs: oviducts, uterus, superior vagina C. external genitalia are indeterminate until week 8 male female genital tubercle penis (glans, corpora cavernosa, clitoris (glans, corpora corpus spongiosum) cavernosa), vestibular bulb) urethral folds fuse to form penile urethra labia minora labioscrotal swellings fuse to form scrotum labia majora urogenital sinus urinary bladder, urethra, prostate, urinary bladder, urethra, seminal vesicles, bulbourethral inferior vagina, vestibular glands glands Strong/Fall 2008 Anatomy Lecture Notes Chapter 24 Male A. gonads = testes (singular = testis) located in scrotum 1. outer coverings a. tunica vaginalis =double layer of serous membrane that partially surrounds each testis; (figure 24.29) b. -
Oogenesis in Mammals
OOGENESIS IN MAMMALS In contrast to most other vertebrates , mammals do not replenish the stores of oocytes present in the ovary at birth. At birth the human ovaries contain about 1 million oocytes ( many of which are already degenerating) that have been arrested in the diplotene stage of the first meiotic division . These oocytes are already surrounded by a layer of follicular cells or granulosa cells , and the complex of ovum and its surrounding cellular investments is known as a follicle . Of all the germ cells present in the ovary ,only about 400 (one per menstrual cycle) will reach maturity and become ovulated. The remainder develop to varying degrees and then undergo atresia (degeneration). Oocytes first become associated with follicular cells in the late fetal period , when they are going through early prophase of the first meiotic division . The primary oocyte (so called because it is undergoing the first meiotic division ) plus its incomplete covering of flattened follicular cells is called a primordial follicle . According to Gougeon (1993) a follicle passes through three major phases on its way to ovulation. The first phase is characterized by a large pool of nongrowing follicles , approximately 500,000 per ovary at birth. In this pool are primordial follicles, which develop into primary follicles by surrounding themselves with a complete single layer of cuboidal follicular cells . By this time , the oocytes have entered the first period of meiotic arrest , the diplotene stage . In human , essentially all oocytes , unless Page 1 of 5 : SEM-2 (GEN ) , Unit#6 : OOGENESIS : Pritha Mondal they degenerate ,remain arrested in the diplotene stage until puberty ; some will not progress past the diplotene stage until the woman’s last reproductive cycle (age 45 to 55 years). -
Characterization of Murine Macrophages from Bone Marrow
Wang et al. BMC Immunology 2013, 14:6 http://www.biomedcentral.com/1471-2172/14/6 RESEARCH ARTICLE Open Access Characterization of murine macrophages from bone marrow, spleen and peritoneum Changqi Wang1*†, Xiao Yu1,2†, Qi Cao1, Ya Wang1, Guoping Zheng1, Thian Kui Tan1, Hong Zhao1,3, Ye Zhao1, Yiping Wang1 and David CH Harris1 Abstract Background: Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these particular sources remain unclear. In this study, the features of murine macrophages from bone marrow, spleen and peritoneum were compared. Results: We found that peritoneal macrophages (PMs) appear to be more mature than bone marrow derived macrophages (BMs) and splenic macrophages (SPMs) based on their morphology and surface molecular characteristics. BMs showed the strongest capacity for both proliferation and phagocytosis among the three populations of macrophage. Under resting conditions, SPMs maintained high levels of pro-inflammatory cytokines expression (IL-6, IL-12 and TNF-α), whereas BMs produced high levels of suppressive cytokines (IL-10 and TGF-β). However, SPMs activated with LPS not only maintained higher levels of (IL-6, IL-12 and TNF-α) than BMs or PMs, but also maintained higher levels of IL-10 and TGF-β. Conclusions: Our results show that BMs, SPMs and PMs are distinct populations with different biological functions, providing clues to guide their further experimental or therapeutic use. Keywords: Macrophage, Bone marrow, Spleen, Peritoneum Background macrophage populations could be attributed to their het- Macrophages play an essential role in both innate and erogeneity [4]. -
GROSS and HISTOMORPHOLOGY of the OVARY of BLACK BENGAL GOAT (Capra Hircus)
VOLUME 7 NO. 1 JANUARY 2016 • pages 37-42 MALAYSIAN JOURNAL OF VETERINARY RESEARCH RE# MJVR – 0006-2015 GROSS AND HISTOMORPHOLOGY OF THE OVARY OF BLACK BENGAL GOAT (Capra hircus) HAQUE Z.1*, HAQUE A.2, PARVEZ M.N.H.3 AND QUASEM M.A.1 1 Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh 2 Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 3 Department of Anatomy and Histology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Basherhat, Dinajpur * Corresponding author: [email protected] ABSTRACT. Ovary plays a vital 130.07 ± 12.53 µm and the oocyte diameter role in the reproductive biology and was 109.8 ± 5.75 µm. These results will be biotechnology of female animals. In this helpful to manipulate ovarian functions in study, both the right and left ovaries of small ruminants. the Black Bengal goat were collected from Keywords: Morphometry, ovarian the slaughter houses of different Thanas follicles, cortex, medulla, oocyte. in the Mymensingh district. For each of the specimens, gross parameters such as INTRODUCTION weight, length and width were recorded. Then they were processed and stained with Black Bengal goat is the national pride of H&E for histomorphometry. This study Bangladesh. The most promising prospect revealed that the right ovary (0.53 ± 0.02 of Black Bengal goat in Bangladesh is g) was heavier than the left (0.52 ± 0.02 g). that this dwarf breed is a prolific breed, The length of the right ovary (1.26 ± 0.04 requiring only a small area to breed and cm) was lower than the left (1.28 ± 0.02 with the advantage of their selective cm) but the width of the right (0.94 ± 0.02 feeding habit with a broader feed range. -
Human General Histology
135 اووم څپرکي انډوکراين سيستم (Endocrine system) (hormones) (target cells) (receptors) autonomic (sinusoids) ductless glands thyroid gland Pineal gland Hypophysis cerebri(pituitary glands) supra renal( adrenal glands) parathyroid glands 136 اووم څپرکي انډوکراين سيسټم islets cell corpora lutea interstitial tissue (placenta) GIT amines neurotransmitters amines neuromodulator 137 اووم څپرکي انډوکراين سيسټم APUD cells neuroendocrine system system adrenaline, (amino acid derivatives) thyroxin noradrenalin thyroid vasopressin encephalin (small peptides) releasing hormone(TRH) TSH(thyroid stimulating parathormone hormone) cortisol Testosterone estrogen (steroids) 5,12,3 (Hypophysis Cerebri) (brain) pituitary gland (stalk) Ventricle infundibulum stalk pituitary fossa sphenoid pineal hypothalamus body 138 اووم څپرکي انډوکراين سيسټم Hypophysis cerebri Pars pars anterior pars nervosa pars posterior intermediate hypothalamus infundibulum infundibulum stalk )Pars posterior neurohypophysis median eminence (tuber cinereum) infundibulum pars neurohypophysis median eminence pars intermediate pars distalis anterior Adenohypophysis infundibulum pars anterior pars tuberalis adenohypophysis 139 اووم څپرکي انډوکراين سيسټم Adenohypophysis pars intermediate pars anterior adenohypophyisis Pars anterior fenestrated sinusoids (cords) chromophil chromophobic acidophil chromophil basophils orange G eosin PAS-positive hematoxylline Beta cells basophil Alpha cells Acidophil basophils acidophil (dese cored vesicles) alpha Beta Histochemical 140 اووم څپرکي انډوکراين -
Regulation and Roles of the Hyaluronan System in Mammalian Reproduction
REPRODUCTIONREVIEW Regulation and roles of the hyaluronan system in mammalian reproduction Ali A Fouladi-Nashta1, Kabir A Raheem1,2, Waleed F Marei1,3, Fataneh Ghafari1 and Geraldine M Hartshorne4 1Royal Veterinary College, Reproduction Research Group, Hawkshead Campus, Hatfield, UK, 2Department of Veterinary Surgery and Theriogenology, Michael Okpara University of Agriculture, Umudike, Nigeria, 3Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt and 4Warwick Medical School, University of Warwick, Coventry, UK and Centre for Reproductive Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, UK Correspondence should be addressed to A A Fouladi-Nashta; Email: [email protected] Abstract Hyaluronan (HA) is a non-sulphated glycosaminoglycan polymer naturally occurring in many tissues and fluids of mammals, including the reproductive system. Its biosynthesis by HA synthase (HAS1–3) and catabolism by hyaluronidases (HYALs) are affected by ovarian steroid hormones. Depending upon its molecular size, HA functions both as a structural component of tissues in the form of high-molecular-weight HA or as a signalling molecule in the form of small HA molecules or HA fragments with effects mediated through interaction with its specific cell-membrane receptors. HA is produced by oocytes and embryos and in various segments of the reproductive system. This review provides information about the expression and function of members of the HA system, including HAS, HYALs and HA receptors. We examine their role in various processes from folliculogenesis through oocyte maturation, fertilisation and early embryo development, to pregnancy and cervical dilation, as well as its application in assisted reproduction technologies. Particular emphasis has been placed upon the role of the HA system in pre-implantation embryo development and embryo implantation, for which we propose a hypothetical sequential model. -
1 | Page: Immune Cells and Tissues Swailes Cells and Tissues of The
Cells and Tissues of the Immune System N. Swailes, Ph.D. Department of Anatomy and Cell Biology Rm: B046A ML Tel: 5-7726 E-mail: [email protected] Required reading Mescher AL, Junqueira’s Basic Histology Text and Atlas, 12th Edition, Chapter 20: pp226-2480 Ross MH and Pawlina W, Histology: A text and Atlas, 6th Edition, Chapter 21: pp396-429 Learning objectives 1) Identify the major cells of the immune system and briefly outline their function 2) Describe the general structure of lymphoid tissue 3) Differentiate between primary and secondary immune organs 4) Identify the thymus and discuss the role of its cells in ‘educating’ immature T-cells 5) Identify a lymph node and outline how an immune response is triggered here 6) Identify the spleen and describe the role of red and white pulp in filtering the blood and reacting to blood borne antigens 7) Differentiate between MALT in the oral cavity (tonsils) 8) Know where to find and how to identify examples of BALT and GALT Major Take Home Points A. Lymphoid tissues are composed of different types of lymphocytes and supporting cells within a scaffold of Type III collagen/reticular fibers B. The major primary lymphoid organs are encapsulated organs where immature lymphocytes are born (bone marrow) and become immunocompetent (thymus) C. The major secondary lymphoid organs are encapsulated organs where immunocompetent lymphocytes differentiate into effector cells after exposure to antigen in the blood (spleen) or lymph (nodes) D. Mucosa Associated Lymphoid Tissues (MALT) are un-encapsulated areas of lymphoid tissue within the mucosa of organs that can be identified by their lining epithelium (tonsils, GALT: ileum and appendix, BALT) 1 | Page: Immune Cells and Tissues Swailes A1: Organization of the immune system 5a A. -
Pineal Calcification, Melatonin Production, Aging, Associated
molecules Review Pineal Calcification, Melatonin Production, Aging, Associated Health Consequences and Rejuvenation of the Pineal Gland Dun Xian Tan *, Bing Xu, Xinjia Zhou and Russel J. Reiter * Department of Cell Systems & Anatomy, UT Health San Antonio, San Antonio, TX 78229, USA; [email protected] (B.X.); [email protected] (X.Z.) * Correspondence: [email protected] (D.X.T.); [email protected] (R.J.R.); Tel.: +210-567-2550 (D.X.T.); +210-567-3859 (R.J.R.) Received: 13 January 2018; Accepted: 26 January 2018; Published: 31 January 2018 Abstract: The pineal gland is a unique organ that synthesizes melatonin as the signaling molecule of natural photoperiodic environment and as a potent neuronal protective antioxidant. An intact and functional pineal gland is necessary for preserving optimal human health. Unfortunately, this gland has the highest calcification rate among all organs and tissues of the human body. Pineal calcification jeopardizes melatonin’s synthetic capacity and is associated with a variety of neuronal diseases. In the current review, we summarized the potential mechanisms of how this process may occur under pathological conditions or during aging. We hypothesized that pineal calcification is an active process and resembles in some respects of bone formation. The mesenchymal stem cells and melatonin participate in this process. Finally, we suggest that preservation of pineal health can be achieved by retarding its premature calcification or even rejuvenating the calcified gland. Keywords: pineal gland; calcification; melatonin; aging; neurodegenerative diseases; rejuvenation 1. Introduction Pineal gland is a unique organ which is localized in the geometric center of the human brain. Its size is individually variable and the average weight of pineal gland in human is around 150 mg [1], the size of a soybean. -
Ans 214 SI Multiple Choice Set 4 Weeks 10/14 - 10/23
AnS 214 SI Multiple Choice Set 4 Weeks 10/14 - 10/23 The following multiple choice questions pertain to material covered in the last two weeks' lecture sets. Answering the following questions will aid your exam preparation. These questions are meant as a gauge of your content knowledge - use them to pinpoint areas where you need more preparation. 1. A heifer begins ovarian activity at A. 6-8 months B. 8-10 months C.10-12 months D. 12-14 months E. 24 months 2. The gestation length of a cow is A. 82 days C. 166 days D. 283 days E. 311 days 3. All of the following produce hormones vital to ovarian cyclicity EXCEPT A. hypothalamus B. ovary C. posterior pituitary D. uterus E. all of the above are correct 4. Which of the following structures is INCORRECTLY matched to the hormones it produces? A. uterus: PGF2a B. ovary: testosterone, activin, estrogen, oxytocin C. placenta: progesterone, testosterone, estrogen D. anterior pituitary: ACTH, FSH, LH E. hypothalamus: GnRH, CRH 5. In the female reproductive system of all species A. the ovaries are supported by the mesometrium B. urine can only exit via the urethra via the suburethral diverticulum C. the uterus produces progesterone D. the oviduct is supported by the mesosalpinx E. the ovary is directly connected to the oviduct 6. Which of the following is FALSE about the mare? A. Ovulates from the medulla because of an inverted ovarian structure B. Ovulates a 2n oocyte C. Does not have a suburethral diverticulum D. Ovulates at only one site on the ovary, called the ovulation fossa E. -
And Theca Interna Cells from Developing Preovulatory Follicles of Pigs B
Differential production of steroids by dispersed granulosa and theca interna cells from developing preovulatory follicles of pigs B. K. Tsang, L. Ainsworth, B. R. Downey and G. J. Marcus * Reproductive Biology Unit, Department of Obstetrics and Gynecology and Department of Physiology, University of Ottawa, Ottawa Civic Hospital, Ottawa, Ontario, Canada Kl Y 4E9 ; tAnimal Research Centre, Agriculture Canada, Ottawa, Ontario, Canada K1A 0C6; and \Department of Animal Science, Macdonald College of McGill University, Ste Anne de Bellevue, Quebec, Canada H9X ICO Summary. Dispersed granulosa and theca interna cells were recovered from follicles of prepubertal gilts at 36, 72 and 108 h after treatment with 750 i.u. PMSG, followed 72 h later with 500 i.u. hCG to stimulate follicular growth and ovulation. In the absence of aromatizable substrate, theca interna cells produced substantially more oestrogen than did granulosa cells. Oestrogen production was increased markedly in the presence of androstenedione and testosterone in granulosa cells but only to a limited extent in theca interna cells. The ability of both cellular compartments to produce oestrogen increased up to 72 h with androstenedione being the preferred substrate. Oestrogen production by the two cell types incubated together was greater than the sum produced when incubated alone. Theca interna cells were the principal source of androgen, predominantly androstenedione. Thecal androgen production increased with follicular development and was enhanced by addition of pregnenolone or by LH 36 and 72 h after PMSG treatment. The ability of granulosa and thecal cells to produce progesterone increased with follicular development and addition of pregnenolone. After exposure of developing follicles to hCG in vivo, both cell types lost their ability to produce oestrogen. -
Histology Histology
HISTOLOGY HISTOLOGY ОДЕСЬКИЙ НАЦІОНАЛЬНИЙ МЕДИЧНИЙ УНІВЕРСИТЕТ THE ODESSA NATIONAL MEDICAL UNIVERSITY Áiáëiîòåêà ñòóäåíòà-ìåäèêà Medical Student’s Library Серія заснована в 1999 р. на честь 100-річчя Одеського державного медичного університету (1900–2000 рр.) The series is initiated in 1999 to mark the Centenary of the Odessa State Medical University (1900–2000) 1 L. V. Arnautova O. A. Ulyantseva HISTÎLÎGY A course of lectures A manual Odessa The Odessa National Medical University 2011 UDC 616-018: 378.16 BBC 28.8я73 Series “Medical Student’s Library” Initiated in 1999 Authors: L. V. Arnautova, O. A. Ulyantseva Reviewers: Professor V. I. Shepitko, MD, the head of the Department of Histology, Cytology and Embryology of the Ukrainian Medical Stomatologic Academy Professor O. Yu. Shapovalova, MD, the head of the Department of Histology, Cytology and Embryology of the Crimean State Medical University named after S. I. Georgiyevsky It is published according to the decision of the Central Coordinational Methodical Committee of the Odessa National Medical University Proceedings N1 from 22.09.2010 Навчальний посібник містить лекції з гістології, цитології та ембріології у відповідності до програми. Викладено матеріали теоретичного курсу по всіх темах загальної та спеціальної гістології та ембріології. Посібник призначений для підготовки студентів до практичних занять та ліцензійного екзамену “Крок-1”. Arnautova L. V. Histology. A course of lectures : a manual / L. V. Arnautova, O. A. Ulyantseva. — Оdessa : The Оdessa National Medical University, 2010. — 336 p. — (Series “Medical Student’s Library”). ISBN 978-966-443-034-7 The manual contains the lecture course on histology, cytology and embryol- ogy in correspondence with the program. -
Interactions Between Oocyte and Surrounding Cumulus Cells Influence the Results of Assisted Reproduction Fritzsche H, Michelmann HW, Siebzehnrübl E Schmedemann RKA J
Journal für Reproduktionsmedizin und Endokrinologie – Journal of Reproductive Medicine and Endocrinology – Andrologie • Embryologie & Biologie • Endokrinologie • Ethik & Recht • Genetik Gynäkologie • Kontrazeption • Psychosomatik • Reproduktionsmedizin • Urologie Interactions between Oocyte and Surrounding Cumulus Cells Influence the Results of Assisted Reproduction Fritzsche H, Michelmann HW, Siebzehnrübl E Schmedemann RKA J. Reproduktionsmed. Endokrinol 2006; 3 (6), 373-378 www.kup.at/repromedizin Online-Datenbank mit Autoren- und Stichwortsuche Offizielles Organ: AGRBM, BRZ, DVR, DGA, DGGEF, DGRM, D·I·R, EFA, OEGRM, SRBM/DGE Indexed in EMBASE/Excerpta Medica/Scopus Krause & Pachernegg GmbH, Verlag für Medizin und Wirtschaft, A-3003 Gablitz Interactions between Oocyte and Surrounding Cumulus Cells Influence the Results of Assisted Reproduction H. Fritzsche1, H. W. Michelmann2, E. Siebzehnrübl3, R. K. A. Schmedemann4 The interactions between oocyte and surrounding cumulus cells, as well as between cumulus oophorus and theca cells, were investigated in IVF/ ICSI cycles. Gap junctions connect cumulus cells with the oocyte, thereby enabling a bi-directional exchange of products essential for optimal oocyte development. GnRH, FSH, LH and E2 play a major role during oocyte maturation. In general, FSH and LH are prerequisites for folliculogenesis, as well as oogenesis, but it is the quantitative threshold value of both that seems to determine oocyte quality and pregnancy rate. It remains to be determined how apoptosis and the anti-Muellerian hormone (AMH) can be used as predictive factors regarding the success of ART. In a retrospec- tive sub-analysis of comparative stimulation regimens, using either LH + FSH (hMG-HP) or FSH (rFSH) alone in GnRH-antagonist down-regulated cycles, it was possible to demonstrate that stimulation with LH and FSH results in a significantly higher pregnancy rate in IVF-patients compared to a stimulation with only FSH.