Human EIF5A (His Tag)

Catalog Number: 15096-H07E

General Information SDS-PAGE:

Gene Name Synonym:

EIF-5A; EIF5A1; eIF5AI

Protein Construction:

A DNA sequence encoding the human EIF5A (NP_001961.1) (Met1- Lys154) was expressed with a polyhistidine tag at the N-terminus.

Source: Human Expression Host: E. coli QC Testing

Purity: > 85 % as determined by SDS-PAGE Endotoxin: Protein Description Please contact us for more information. EIF-5A, also known as EIF5, functions in start site selection as a GTPase Stability: accelerating protein (GAP) for the eukaryotic (eIF) 2•GTP•tRNA ternary complex within the -bound pre-initiation ℃ Samples are stable for up to twelve months from date of receipt at -70 complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and His Predicted N terminal: is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding Molecular Mass: of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and The recombinant human EIF5A consists of 170 amino acids and predicts a binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S molecular mass of 18.9 kDa. It migrates as an approximately 21 kDa band ribosome subunit. in SDS-PAGE under reducing conditions. References Formulation: 1.Jenkins ZA. et al., 2001, Genomics. 71 (1): 101-9. 2.Guan XY. et al., Lyophilized from sterile PBS, pH 7.4. 2001, Cancer Res. 61 (9): 3806-9. 3.Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements. Usage Guide

Storage:

Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.

Avoid repeated freeze-thaw cycles.

Reconstitution:

Detailed reconstitution instructions are sent along with the products.

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