For Research Use Only HSP90AB1 Polyclonal antibody www.ptgcn.com Catalog Number:11405-1-AP 12 Publications

Catalog Number: GenBank Accession Number: Recommended Dilutions: Basic Information 11405-1-AP BC014485 WB 1:2000-1:10000 Size: GeneID (NCBI): IP 0.5-4.0 ug for IP and 1:500-1:1000 300 μg/ml 3326 for WB IHC 1:250-1:1000 Source: Full Name: IF 1:10-1:100 Rabbit heat shock protein 90kDa alpha Isotype: (cytosolic), class B member 1 IgG Calculated MW: Purification Method: 723 aa, 83 kDa Antigen affinity purification Observed MW: Immunogen Catalog Number: 83-90 kDa AG1947

Applications Tested Applications: Positive Controls: FC, IF, IHC, IP, WB,ELISA WB : PC-3 cells; mouse brain tissue, Jurkat cells Cited Applications: IP : NIH/3T3 cells; CoIP, IF, IHC, WB IHC : human pancreas cancer tissue; human breast Species Specificity: cancer tissue human, mouse, rat Cited Species: IF : HepG2 cells; deer, human, mouse, pig, sheep Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha Background Information (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Notable Publications Author Pubmed ID Journal Application Thomas Labadie 33075107 PLoS Pathog IF Baoming Xie 32160078 Autophagy WB Yan Sun 32323753 Int J Mol Med WB,IHC

Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 4006900926 E: [email protected] W: ptgcn.com Group brand and is not available to purchase from any other manufacturer. Selected Validation Data

Various lysates were subjected to SDS PAGE Various lysates were subjected to SDS PAGE Immunofluorescent analysis of HepG2 cells, using followed by western blot with 11405-1-AP followed by western blot with 11405-1-AP HSP90AB1 antibody 11405-1-AP at 1:25 dilution and (HSP90AB1 antibody) at dilution of 1:5000 (HSP90AB1 antibody) at dilution of 1:2000 Rhodamine-labeled goat anti-rabbit IgG (red). incubated at room temperature for 1.5 hours. incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin- Immunohistochemical analysis of paraffin- IP Result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; embedded human pancreas cancer tissue slide embedded human pancreas cancer tissue slide Detection:11405-1-AP 1:700) with NIH/3T3 cells using 11405-1-AP (HSP90AB1 antibody) at dilution using 11405-1-AP (HSP90AB1 antibody) at dilution lysate 1200ug. of 1:500 (under 10x lens. Heat mediated antigen of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were stained with 0.2ug HSP90AB1 antibody (11405-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.