DNA Barcoding Facilitates a Rapid Biotic Survey of a Temperate Nature
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Bon Echo Provincial Park
BON ECHO PROVINCIAL PARK One Malaise trap was deployed at Bon Echo Provincial Park in 2014 (44.89405, -77.19691 278m ASL; Figure 1). This trap collected arthropods for twenty weeks from May 7 – September 24, 2014. All 10 Malaise trap samples were processed; every other sample was analyzed using the individual specimen protocol while the second half was analyzed via bulk analysis. A total of 2559 BINs were obtained. Over half the BINs captured were flies (Diptera), followed by bees, ants and wasps (Hymenoptera), moths and butterflies (Lepidoptera), and beetles (Coleoptera; Figure 2). In total, 547 arthropod species were named, representing 22.9% of the BINs from the site (Appendix 1). All BINs were assigned at least to Figure 1. Malaise trap deployed at Bon Echo family, and 57.2% were assigned to a genus (Appendix Provincial Park in 2014. 2). Specimens collected from Bon Echo represent 223 different families and 651 genera. Diptera Hymenoptera Lepidoptera Coleoptera Hemiptera Mesostigmata Trombidiformes Psocodea Sarcoptiformes Trichoptera Araneae Entomobryomorpha Symphypleona Thysanoptera Neuroptera Opiliones Mecoptera Orthoptera Plecoptera Julida Odonata Stylommatophora Figure 2. Taxonomy breakdown of BINs captured in the Malaise trap at Bon Echo. APPENDIX 1. TAXONOMY REPORT Class Order Family Genus Species Arachnida Araneae Clubionidae Clubiona Clubiona obesa Linyphiidae Ceraticelus Ceraticelus atriceps Neriene Neriene radiata Philodromidae Philodromus Salticidae Pelegrina Pelegrina proterva Tetragnathidae Tetragnatha Tetragnatha shoshone -
ARTHROPOD COMMUNITIES and PASSERINE DIET: EFFECTS of SHRUB EXPANSION in WESTERN ALASKA by Molly Tankersley Mcdermott, B.A./B.S
Arthropod communities and passerine diet: effects of shrub expansion in Western Alaska Item Type Thesis Authors McDermott, Molly Tankersley Download date 26/09/2021 06:13:39 Link to Item http://hdl.handle.net/11122/7893 ARTHROPOD COMMUNITIES AND PASSERINE DIET: EFFECTS OF SHRUB EXPANSION IN WESTERN ALASKA By Molly Tankersley McDermott, B.A./B.S. A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biological Sciences University of Alaska Fairbanks August 2017 APPROVED: Pat Doak, Committee Chair Greg Breed, Committee Member Colleen Handel, Committee Member Christa Mulder, Committee Member Kris Hundertmark, Chair Department o f Biology and Wildlife Paul Layer, Dean College o f Natural Science and Mathematics Michael Castellini, Dean of the Graduate School ABSTRACT Across the Arctic, taller woody shrubs, particularly willow (Salix spp.), birch (Betula spp.), and alder (Alnus spp.), have been expanding rapidly onto tundra. Changes in vegetation structure can alter the physical habitat structure, thermal environment, and food available to arthropods, which play an important role in the structure and functioning of Arctic ecosystems. Not only do they provide key ecosystem services such as pollination and nutrient cycling, they are an essential food source for migratory birds. In this study I examined the relationships between the abundance, diversity, and community composition of arthropods and the height and cover of several shrub species across a tundra-shrub gradient in northwestern Alaska. To characterize nestling diet of common passerines that occupy this gradient, I used next-generation sequencing of fecal matter. Willow cover was strongly and consistently associated with abundance and biomass of arthropods and significant shifts in arthropod community composition and diversity. -
The 2014 Golden Gate National Parks Bioblitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event
National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 ON THIS PAGE Photograph of BioBlitz participants conducting data entry into iNaturalist. Photograph courtesy of the National Park Service. ON THE COVER Photograph of BioBlitz participants collecting aquatic species data in the Presidio of San Francisco. Photograph courtesy of National Park Service. The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 Elizabeth Edson1, Michelle O’Herron1, Alison Forrestel2, Daniel George3 1Golden Gate Parks Conservancy Building 201 Fort Mason San Francisco, CA 94129 2National Park Service. Golden Gate National Recreation Area Fort Cronkhite, Bldg. 1061 Sausalito, CA 94965 3National Park Service. San Francisco Bay Area Network Inventory & Monitoring Program Manager Fort Cronkhite, Bldg. 1063 Sausalito, CA 94965 March 2016 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service. -
SYSTEMATICS of the MEGADIVERSE SUPERFAMILY GELECHIOIDEA (INSECTA: LEPIDOPTEA) DISSERTATION Presented in Partial Fulfillment of T
SYSTEMATICS OF THE MEGADIVERSE SUPERFAMILY GELECHIOIDEA (INSECTA: LEPIDOPTEA) DISSERTATION Presented in Partial Fulfillment of the Requirements for The Degree of Doctor of Philosophy in the Graduate School of The Ohio State University By Sibyl Rae Bucheli, M.S. ***** The Ohio State University 2005 Dissertation Committee: Approved by Dr. John W. Wenzel, Advisor Dr. Daniel Herms Dr. Hans Klompen _________________________________ Dr. Steven C. Passoa Advisor Graduate Program in Entomology ABSTRACT The phylogenetics, systematics, taxonomy, and biology of Gelechioidea (Insecta: Lepidoptera) are investigated. This superfamily is probably the second largest in all of Lepidoptera, and it remains one of the least well known. Taxonomy of Gelechioidea has been unstable historically, and definitions vary at the family and subfamily levels. In Chapters Two and Three, I review the taxonomy of Gelechioidea and characters that have been important, with attention to what characters or terms were used by different authors. I revise the coding of characters that are already in the literature, and provide new data as well. Chapter Four provides the first phylogenetic analysis of Gelechioidea to include molecular data. I combine novel DNA sequence data from Cytochrome oxidase I and II with morphological matrices for exemplar species. The results challenge current concepts of Gelechioidea, suggesting that traditional morphological characters that have united taxa may not be homologous structures and are in need of further investigation. Resolution of this problem will require more detailed analysis and more thorough characterization of certain lineages. To begin this task, I conduct in Chapter Five an in- depth study of morphological evolution, host-plant selection, and geographical distribution of a medium-sized genus Depressaria Haworth (Depressariinae), larvae of ii which generally feed on plants in the families Asteraceae and Apiaceae. -
Chorioactidaceae: a New Family in the Pezizales (Ascomycota) with Four Genera
mycological research 112 (2008) 513–527 journal homepage: www.elsevier.com/locate/mycres Chorioactidaceae: a new family in the Pezizales (Ascomycota) with four genera Donald H. PFISTER*, Caroline SLATER, Karen HANSENy Harvard University Herbaria – Farlow Herbarium of Cryptogamic Botany, Department of Organismic and Evolutionary Biology, Harvard University, 22 Divinity Avenue, Cambridge, MA 02138, USA article info abstract Article history: Molecular phylogenetic and comparative morphological studies provide evidence for the Received 15 June 2007 recognition of a new family, Chorioactidaceae, in the Pezizales. Four genera are placed in Received in revised form the family: Chorioactis, Desmazierella, Neournula, and Wolfina. Based on parsimony, like- 1 November 2007 lihood, and Bayesian analyses of LSU, SSU, and RPB2 sequence data, Chorioactidaceae repre- Accepted 29 November 2007 sents a sister clade to the Sarcosomataceae, to which some of these taxa were previously Corresponding Editor: referred. Morphologically these genera are similar in pigmentation, excipular construction, H. Thorsten Lumbsch and asci, which mostly have terminal opercula and rounded, sometimes forked, bases without croziers. Ascospores have cyanophilic walls or cyanophilic surface ornamentation Keywords: in the form of ridges or warts. So far as is known the ascospores and the cells of the LSU paraphyses of all species are multinucleate. The six species recognized in these four genera RPB2 all have limited geographical distributions in the northern hemisphere. Sarcoscyphaceae ª 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. Sarcosomataceae SSU Introduction indicated a relationship of these taxa to the Sarcosomataceae and discussed the group as the Chorioactis clade. Only six spe- The Pezizales, operculate cup-fungi, have been put on rela- cies are assigned to these genera, most of which are infre- tively stable phylogenetic footing as summarized by Hansen quently collected. -
Final Report 1
Sand pit for Biodiversity at Cep II quarry Researcher: Klára Řehounková Research group: Petr Bogusch, David Boukal, Milan Boukal, Lukáš Čížek, František Grycz, Petr Hesoun, Kamila Lencová, Anna Lepšová, Jan Máca, Pavel Marhoul, Klára Řehounková, Jiří Řehounek, Lenka Schmidtmayerová, Robert Tropek Březen – září 2012 Abstract We compared the effect of restoration status (technical reclamation, spontaneous succession, disturbed succession) on the communities of vascular plants and assemblages of arthropods in CEP II sand pit (T řebo ňsko region, SW part of the Czech Republic) to evaluate their biodiversity and conservation potential. We also studied the experimental restoration of psammophytic grasslands to compare the impact of two near-natural restoration methods (spontaneous and assisted succession) to establishment of target species. The sand pit comprises stages of 2 to 30 years since site abandonment with moisture gradient from wet to dry habitats. In all studied groups, i.e. vascular pants and arthropods, open spontaneously revegetated sites continuously disturbed by intensive recreation activities hosted the largest proportion of target and endangered species which occurred less in the more closed spontaneously revegetated sites and which were nearly absent in technically reclaimed sites. Out results provide clear evidence that the mosaics of spontaneously established forests habitats and open sand habitats are the most valuable stands from the conservation point of view. It has been documented that no expensive technical reclamations are needed to restore post-mining sites which can serve as secondary habitats for many endangered and declining species. The experimental restoration of rare and endangered plant communities seems to be efficient and promising method for a future large-scale restoration projects in abandoned sand pits. -
The Ascomycota
Papers and Proceedings of the Royal Society of Tasmania, Volume 139, 2005 49 A PRELIMINARY CENSUS OF THE MACROFUNGI OF MT WELLINGTON, TASMANIA – THE ASCOMYCOTA by Genevieve M. Gates and David A. Ratkowsky (with one appendix) Gates, G. M. & Ratkowsky, D. A. 2005 (16:xii): A preliminary census of the macrofungi of Mt Wellington, Tasmania – the Ascomycota. Papers and Proceedings of the Royal Society of Tasmania 139: 49–52. ISSN 0080-4703. School of Plant Science, University of Tasmania, Private Bag 55, Hobart, Tasmania 7001, Australia (GMG*); School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia (DAR). *Author for correspondence. This work continues the process of documenting the macrofungi of Mt Wellington. Two earlier publications were concerned with the gilled and non-gilled Basidiomycota, respectively, excluding the sequestrate species. The present work deals with the non-sequestrate Ascomycota, of which 42 species were found on Mt Wellington. Key Words: Macrofungi, Mt Wellington (Tasmania), Ascomycota, cup fungi, disc fungi. INTRODUCTION For the purposes of this survey, all Ascomycota having a conspicuous fruiting body were considered, excluding Two earlier papers in the preliminary documentation of the endophytes. Material collected during forays was described macrofungi of Mt Wellington, Tasmania, were confined macroscopically shortly after collection, and examined to the ‘agarics’ (gilled fungi) and the non-gilled species, microscopically to obtain details such as the size of the -
Boyne Valley Provincial Park
BOYNE VALLEY PROVINCIAL PARK One Malaise trap was deployed at Boyne Valley Provincial Park in 2014 (44.11563, -80.12777, 468m ASL; Figure 1). This trap collected arthropods for twenty weeks from April 28 – September 19, 2014. All 10 Malaise trap samples were processed; every other sample was analyzed using the individual specimen protocol while the second half was analyzed via bulk analysis. A total of 1571 BINs were obtained. Over half the BINs captured were flies (Diptera), followed by bees, ants and wasps (Hymenoptera), moths and butterflies (Lepidoptera), and beetles (Coleoptera; Figure 2). In total, 427 arthropod species were named, representing 29% of the BINs from the site (Appendix 1). All BINs were assigned at least to Figure 1. Malaise trap deployed at Boyne Valley family, and 66.6% were assigned to a genus (Appendix Provincial Park in 2014. 2). Specimens collected from Boyne Valley represent 183 different families and 558 genera. Figure 2. Taxonomy breakdown of BINs captured in the Malaise trap at Boyne Valley. APPENDIX 1. TAXONOMY REPORT Class Order Family Genus Species Arachnida Araneae Clubionidae Clubiona Clubiona obesa Philodromidae Philodromus Philodromus rufus Theridiidae Mesostigmata Digamasellidae Dinychidae Halolaelapidae Parasitidae Phytoseiidae Opiliones Phalangiidae Sclerosomatidae Leiobunum Sarcoptiformes Acaridae Oribatulidae Phenopelopidae Scheloribatidae Trombidiformes Anystidae Cunaxidae Cunaxoides Erythraeidae Leptus Hygrobatidae Atractides Scutacaridae Tarsonemidae Tetranychidae Tetranychus Trombidiidae -
Species of Peziza S. Str. on Water-Soaked Wood with Special Reference to a New Species, P
DOI 10.12905/0380.sydowia68-2016-0173 Species of Peziza s. str. on water-soaked wood with special reference to a new species, P. nordica, from central Norway Donald H. Pfister1, *, Katherine F. LoBuglio1 & Roy Kristiansen2 1 Department of Organismic and Evolutionary Biology, Harvard University Herbaria, 22 Divinity Ave., Cambridge, MA 02138, USA 2 PO Box 32, N-1650 Sellebakk, Norway * e-mail: [email protected] Pfister D.H., LoBuglio K.F. & Kristiansen R. (2016) Species ofPeziza s. str. on water-soaked wood with special reference to a new species, P. nordica, from central Norway. – Sydowia 68: 173–185. Peziza oliviae, P. lohjaoensis, P. montirivicola and a new species from Norway form a well-supported clade within the Peziza s. str. group based on study of the internal transcribed spacer + 5.8S rRNA gene, large subunit rRNA gene and the 6–7 region of the DNA-dependent RNA polymerase II gene. Like P. oliviae and P. montirivicola, the new species, P. nordica, is distinctly stipi- tate and occurs on wood that has been inundated by fresh water. These species also have paraphyses with yellow vacuolar inclu- sions. They fruit early in the season or at high elevations and are presumed to be saprobic. A discussion of application of the name Peziza is given. Keywords: Ascomycota, molecular phylogeny, Pezizales, taxonomy. The present work was begun to determine the Schwein.) Fr., Cudoniella clavus (Alb. & Schwein.) identity of a collection made by one of us (RK) in Dennis and frequently Scutellinia scutellata (L.) August 2014. This large, orange brown to brown, Lambotte. -
Records of Two New Palearctic Moth Species Associated with Queen Anne’S Lace in Nova Scotia
J. Acad. Entomol. Soc. 13: 54-57 (2017) NOTE Records of two new Palearctic moth species associated with Queen Anne’s lace in Nova Scotia Jeffrey Ogden Daucus carota Linnaeus, commonly known as Queen Anne’s lace or wild carrot, is native to Europe and southwestern Asia. It is believed to have been introduced to North America in soil ballast by the first European settlers (Lindroth 1957). It has historically been used as a food source and is the ancestral plant of our common cultivated garden carrot. Since its introduction, Daucus carota has spread widely and is common to much of North America. In Nova Scotia, this biennial is a very common plant of fields, roadsides and other weedy areas throughout the mainland and parts of Cape Breton (Zinck 1998). Previous insect surveys have recorded hundreds of species of insects attracted to the large white flower heads of the plant (Judd 1970; Largo & Mann 1987). Although considered a common weed species, Daucus carota is an important host to numerous beneficial insect species, including many pollinators and predatory species (Judd 1970). In this report, the first detections ofSitochroa palealis (Denis & Schiffermüller) (Lepidoptera: Crambidae), the carrot seed moth, and Depressaria depressana (Fabricius) (Lepidoptera: Depressariidae), the purple carrot seed moth, are described from Nova Scotia. Larvae of each species were collected within the flower heads of Daucus carota during the summers of 2015 to 2017. Adults were collected as part of an ongoing light trapping survey and through lab rearing. Verified photograph records were also considered. Voucher specimens have been deposited in the Nova Scotia Department of Natural Resources Reference Collection, the Nova Scotia Museum, and the author’s personal collection. -
CHECKLIST of WISCONSIN MOTHS (Superfamilies Mimallonoidea, Drepanoidea, Lasiocampoidea, Bombycoidea, Geometroidea, and Noctuoidea)
WISCONSIN ENTOMOLOGICAL SOCIETY SPECIAL PUBLICATION No. 6 JUNE 2018 CHECKLIST OF WISCONSIN MOTHS (Superfamilies Mimallonoidea, Drepanoidea, Lasiocampoidea, Bombycoidea, Geometroidea, and Noctuoidea) Leslie A. Ferge,1 George J. Balogh2 and Kyle E. Johnson3 ABSTRACT A total of 1284 species representing the thirteen families comprising the present checklist have been documented in Wisconsin, including 293 species of Geometridae, 252 species of Erebidae and 584 species of Noctuidae. Distributions are summarized using the six major natural divisions of Wisconsin; adult flight periods and statuses within the state are also reported. Examples of Wisconsin’s diverse native habitat types in each of the natural divisions have been systematically inventoried, and species associated with specialized habitats such as peatland, prairie, barrens and dunes are listed. INTRODUCTION This list is an updated version of the Wisconsin moth checklist by Ferge & Balogh (2000). A considerable amount of new information from has been accumulated in the 18 years since that initial publication. Over sixty species have been added, bringing the total to 1284 in the thirteen families comprising this checklist. These families are estimated to comprise approximately one-half of the state’s total moth fauna. Historical records of Wisconsin moths are relatively meager. Checklists including Wisconsin moths were compiled by Hoy (1883), Rauterberg (1900), Fernekes (1906) and Muttkowski (1907). Hoy's list was restricted to Racine County, the others to Milwaukee County. Records from these publications are of historical interest, but unfortunately few verifiable voucher specimens exist. Unverifiable identifications and minimal label data associated with older museum specimens limit the usefulness of this information. Covell (1970) compiled records of 222 Geometridae species, based on his examination of specimens representing at least 30 counties. -
DNA Barcoding
Full-time PhD studies of Ecology and Environmental Protection Piotr Gadawski Species diversity and origin of non-biting midges (Chironomidae) from a geologically young lake PhD Thesis and its old spring system Performed in Department of Invertebrate Zoology and Hydrobiology in Institute of Ecology and Environmental Protection Różnorodność gatunkowa i pochodzenie fauny Supervisor: ochotkowatych (Chironomidae) z geologicznie Prof. dr hab. Michał Grabowski młodego jeziora i starego systemu źródlisk Auxiliary supervisor: Dr. Matteo Montagna, Assoc. Prof. Łódź, 2020 Łódź, 2020 Table of contents Acknowledgements ..........................................................................................................3 Summary ...........................................................................................................................4 General introduction .........................................................................................................6 Skadar Lake ...................................................................................................................7 Chironomidae ..............................................................................................................10 Species concept and integrative taxonomy .................................................................12 DNA barcoding ...........................................................................................................14 Chapter I. First insight into the diversity and ecology of non-biting midges (Diptera: Chironomidae)