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General Disclaimer One Or More of the Following Statements May Affect General Disclaimer One or more of the Following Statements may affect this Document This document has been reproduced from the best copy furnished by the organizational source. It is being released in the interest of making available as much information as possible. This document may contain data, which exceeds the sheet parameters. It was furnished in this condition by the organizational source and is the best copy available. This document may contain tone-on-tone or color graphs, charts and/or pictures, which have been reproduced in black and white. This document is paginated as submitted by the original source. Portions of this document are not fully legible due to the historical nature of some of the material. However, it is the best reproduction available from the original submission. Produced by the NASA Center for Aerospace Information (CASI) ^Y CO-EXIST NCI : OF LIPID Ai,-IDC /:S E1^it:IJI.I IN ' E3:P%RIA(ita^!'I:^^.f. OI:C0;^4PRF.SSfOP. SlCfa^lCSS I A.'t.I . Cclrl; ai', M-A, S-M- Pauley, hIt.D.. J.C. Saunders, Mi.D. and F.N. Hirosc, I . N69-36'741 IACCXSSION NUMOLRIR RY) :` q tr Rur hoof WASA OR OR TM% OR AO NOM8811I ICATCOORTI From flee Depurinic-nis of Surg::1; /Urology, FICfri.)or Ger,aral l-losp ii•al, Torrance, Ca li fornia, 50504 and JCUA Schrol of 1v1ecicit;^: ; Los Anplcs C1il.i idle Dt'visktri of Jralu3j v, Univorsiiy of Rcchosler School of Rochcsicr, New York. I ,elf (N1'l . Sup/ +! :1' led in ,,r. Ly (-7o • i rocll NP.')-- 1 02-669) b2aw c!c rii the Office of l'•le.vat t'-501 GQ'^h, fife f}G r Cil'i(7Novy CIli F11-1:1 Harbor Goo-'wal Ho pl ilp !l, on(f.a f-rail; frai-1 li)e N 3 :Ai al A eronc ui'ics and 1X4:.0 Adminish-c.iIion 3 •-` `? N a A- DS`UO?-063 A traditional concept of pure nitrogenous bubble embolixation li:As arisen as the etiologic factor in decompression sickness. Quite properly recompression has been employed as the means of treating this syndrome. The resu l ts of treatment were usually dramatic ' 6nd effective. In 1961, we emphasized the importance of hcmoconcentration in altitude type bends cases and suggested the extent of the plasma deficit based on standard clinical data. 1 Subsequent studies by our group employing U mongrel cloys previously splenc-itemized documented' the exiont -of this plash. loss .2 Dextran (LIMI) replucement was effective in treating shock and maintaining an effective circulating blood volume. 3,4 Recompression was deliberately withheld: Th-j.,se early findings suggested to us thot ili .a syndroma of decompression sickness was more complex than the simple bubble theory. LVY/ dextran reverses blood viscosity caused by fibrinogen by fern*.. `ion of a dextran- fibrinogen, complex, This role is in addition to its colloidal, expansive properties. DNV,tran also affects hemostasis; it apparently alters the red blood cell surface by increasin;,j its negativity thus encouraging repulsion of red blood cells. A chance finding subsequently reported by our group s,:omed to clarify our un rl<^rsiandinu of this syndrome. We reported bone marrow emboli in pulmonary arterioles following experim--nial decompression sickness, 5 and sucggesicd? 11ruct a num6ar of conix,li in lice lungs; kidney urid liver wcro of a w 2 « lipid nature. This new wide based approach was amply supporied by our results in treating h nrins with ckncompression sickness. Dextran infusion begun immcdicaely of ar diaE)nosis improved the patients ShOCiail;e ;late and shortened the recompression schedule. Recently we have re-sectioned -lung areas known to be involved by emboli following ex; -arimental decompression. These pulmonary areas were obtained by biopsy or postmortem examination. Pulmonary localiza- tion in 14 dogs wos made possible after the development and refinerscnt of radioisotopic pulmonary scanniny.techniquiCs.6 The purpose of this study is to report the results of tissue sectioning of lungs in animals overcomprossed to 165 feet, maintained at depth for 60 minutes, and then docompressod to surface. Oil•-red••0 stains were made of treated and untreated animals. Treatment consisted of Lh%' dextran or y. intravenous heparin. MATERIALS AND METHODS Thirty-three mongrel dogs vrere, employed in this study. All animals were overcompressod to 165 feet V3.5 PSIG) for 60 minutes. We have previously described our protoc,-,l. ' Five animals unexposed to the chamber were sacrificed to provide baseline glair, 'or routine pulmonary fat stains. .. 3 ... Fourteen animals were treated by dextran following decompression. These anirnals undorvrent lug o biopsy /B hours after the chamber procedure .and immadicifoly following a second lung scan. A third group of M aniMUIS was ovorcompressed in the usual fashion. After chamber removal five animals served as exposed but untreated ecoirols while ten animals received IV heparin 3 hours after chamber removal. The control animals expired. Fat stains were made of lung tissue. Biopsies provided the basis for fat stains in the heparin treated group. RESULTS Unexposr:.d Control Animals (5 clogs) Fat globules may be seen occossionally in lungs of animals previously fasted for 24 hours. The fat globules are di-fficult • .to locate and may be seen in areas containing the smaller blood vessels near the bronchial cartilages. Exposed Untreated Animals (5 clogs) Numerous lipid einboli are easily seen on routine sections (Figures 1 and 21. The emboli appear most frequently in areas where hemorrhage and pulmonary edema are present. Exposed and Treater! Animals_ (24 dogs), Fourteen clogs were treated, by dextran alone. Ten animals were r d r treated by hopvrin alone (fable 1). F:yidcnce of hemorrhage and pulmoncry edema existing in pulmonary areas diagnosed by radioisotopic lung scanning is seen (Figure 3). Fat emboli are also &oen in biopsied areas 48 hours after decompress ion (Figure /). 1 'DISCUSSION 7 Philp ct•al and Partholomy have reported the benefits of heparin as a lipemie Oearing agent in decompression sickness. Survival rates wcrc significantly improved Mill heparin. We recently confirmed the benefits of 9 heparin in experimental decompression sickness lipid erabolization would appear to be significant since recompression was not employed, and heparin was Protect-h . While gaseous embolization is undoubtedly a major factor in the genesis of decompression sickness, our experience with dextran would suggest the more imporiont role of lipid emboli. lipid content is also reduced after dextran treattn:.nt. A resemblance exists bolween truurnatic .fatty emboli ation and decompression sickness. Striking similarities when comparing both clinical syndromes are as follows: 1) A latent period develops before the onset of syrniitoms. 2) Symptomatology are identical in both. They include puhronary edema, hypoxia, shod: and central nervous systern signs. J) Pelechiae in patients with decompression sickness tollo,v skin patterns described in fat ambolisrn. 4) IllheropcM is ren imens are similar. SUMMARY AND CONCLUSIONS 1) Lipid ombolization would appear to play a molar role in flip. genesis of doe omp ression sickness. 2) Evidence for the co-existence of lipid emboli c;nd gm'-- us emboli is presented. 3) Heparin or dextran-effective lipemic clearing agents—arc . beneficial in treating experimental decompression sickness. 4) A .combined therapeutic approach—recompression and dextran should be employed in treating human decompression sickness. Heparin may serve as u substitute in selected instances, LEGENDS Figure 1. Photornicrororap!i d-clonstrating fat emboli in small capillaries in pulmonary region (101OX) Figure 2. Fat erutiolus (oil-red-0) in capillary in lung (125X.) . Figure 3. flernorrhage and pulmoncn•y edema in Ivng of dog orposed to 165 feet for 60 minutes (60X) Figure 4. Fat emboli (oil-red-0) in small vessels surrounding bronchioles (`00X) - REFERENCES Watts-, W.G., J.B. Fitzgerald and A.T.K. Cockott: Dysbarism: A P.oviow with Suclqesllons for Therapy. Aerospace Med. 33:1132, 1962. 2. Cockott, A.T.IC., R.M. Nakamura and JJ. Franks: Delayed Shock in Experimental Dysbarism. Surg. Forum 14:7, 1963. 3. Cockett, A.T.I<. and R.M. Nakamura: Newer Concepts in the Patho- physiology of Experimental Dysbarism-Decompression Sickness. Amer. Surg. 30:447, 1964. to 4. Cockett, A.T.K., R.M. Nakamura and R:T. Kado: Physiological Factors in Decompression Siclness. Arch. Environ., Health 11:760, 1965. 5. Cockett, A.LK., R.M. Nakamura and J.J. Franks: Recent Findinas In the • Pathogenesis of Decompression Sicknoss (Dysbarism). Surgery ,. 58:384,; 1965. 6. CDckedi A.T.K., N.L. Mangelson, L. Swanson an'tl R.T. Kado: The Diagnosis of Experimental Pulmonary Aeroemboli Following Dacompi:ession by Radioisotopic Long Scanning. Amar. Surg. 34:109, 1960. 7. Philp, R.Q., Govedey, C.W.. and M. Prosall: (Ionaes in Blood Lipid Concentration and Coll Counts Following Decompression Sickness in Rats and the Influence of Diatary Lipid. Can. a. Physiol. Pharm. 45:1047, 1967. L. ^i. Parthelerny, L.: Blood Cougulution and Chemistry Dm ing Experimental Divos and the Trectirsient of Diving Accidents with 1-iopurin. Second Sympsitica on Undcrwater Physiolojy, - \Mash. V.C., Nat. Acrid. Sci. Pub. 118146, 19,63. 90 Cocicoii; A.T.I:., J.C. Snunc!crs and S.M. Pauley: Troaimcnt of ` Cxperim:ntal Decompression Sickness by I-lepurin Alone. Presenled at Annual Aliectincg of Aerospaco Med. Assoc., May 7, 1968, San Francisco, Californ.m. Y.
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