Under the Direction of Lisa L. Dean)
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ABSTRACT GRIFFIN, LAURA ELIZABETH. The Sensory and Nutritional Characterization of Cashew Nuts. (Under the direction of Lisa L. Dean). Cashews, known for their pleasing flavor, are the second most popular type of tree nut. While sales of cashew nuts continually grow at an annual rate of 7%, they are neither cultivated in plantations, nor processed with advanced industrial equipment. As such, the trees are left to grow in low-nutrient environments and many cashew varieties exist due to self-pollination and seed dispersal. Cashews are typically consumed as-is, but are also used in cooking and confectionary products worldwide. Processing for direct consumption involves detachment from the cashew apple, shell drying, shelling, removal of caustic cashew nut shell liquid (CNSL), drying, peeling, roasting, and packaging. Oil-roasting is the primary method of roasting and the nuts are almost exclusively sold without the shells and skins, as CNSL can cause allergic reactions in some individuals, despite the skins being a potential source of antioxidants. The unique Karma brand nuts were analyzed for their nutritional content, with special interest in the skin-on product. Proximate analysis indicated that the sugar content of the nuts varied due to processing. Oleic (61.3-61.8%) and linoleic (16.7-17.3%) acids were present in the nuts in the highest levels and the second most abundant amino acid was arginine (1.24-1.57 g/100g), a known vasodilator. The cashews had similar amounts of phytosterols (168.05-192.86 mg/100g) compared to other nuts, but were poor sources of water and fat soluble vitamins. The skin-on nuts did contain some antioxidant activity (25.2 mg Trolox eq/g cashew) but quantification of procyanidins revealed that only monomers were present in the skins. It is likely that the water washing procedure to remove CNSL from the skins also removes some of the larger water soluble phenolics. The amount of procyanidins in the cashew skins (1473.5 mg/100g) was much less than the amount in peanut skins (1500 mg/100g). Given the development of novel cashew products by Karma™, a lexicon was developed to describe cashew flavor. The lexicon, validated by tasting 14 different cashew products, contained 22 flavors, 3 feeling factors, and 4 texture terms. Principal component analysis of the cashew products indicated that Harris Teeter store brand cashews were most associated with the on-flavors found in cashews. Cashews with skins were highest in “woody”, “bitter”, and “astringent” flavors, while rancid dry roasted samples were most associated with “cardboardy” and “painty” flavors. Raw cashews were highest in “other nut meat”, “fruity”, and “raw, beany” flavors. Further consumer comparison of raw, dry roasted, skin-on, and oil roasted cashews yielded information regarding liking of different processing methods. Acceptance testing results from 102 panelists indicated that the oil-roasted cashews were most liked in terms of appearance, overall flavor, sweetness, saltiness, and texture. The oil roasted sample was also ranked the highest out of the four products. The skin-on cashew appearance was “disliked slightly”, indicating that despite potential health benefits, visual appeal could impact sales. The raw cashews, although having the same overall flavor liking score as all other nuts received the lowest rank. These observations indicated that nuts that have been roasted in some way were preferred. Preference mapping did indicate however, that one cluster of consumers in the panel did prefer the raw and skin on products, indicating that these unique nuts would perform best in niche markets. Cashew nuts have a unique sensory profile and different products appealed to different groups of consumers. Although they do contain compounds known to reduce heart disease risk factors such as phytosterols, magnesium, and unsaturated fatty acids, the amounts in cashew nuts are lower than the amounts found in other nuts such as pistachios, peanuts, or almonds. © Copyright 2016 Laura Elizabeth Griffin All Rights Reserved The Sensory and Nutritional Characterization of Cashew Nuts by Laura Elizabeth Griffin A thesis submitted to the Graduate Faculty of North Carolina State University in partial fulfillment of the requirements for the degree of Master of Science Food Science Raleigh, North Carolina 2016 APPROVED BY: _______________________________ ______________________________ Lisa L. Dean Mary Anne Drake Committee Chair ________________________________ ________________________________ Gabriel Keith Harris Anastasios Tsiatis BIOGRAPHY Laura Griffin’s passion for food science and research began in high school, where she conducted research on red cabbage anthocyanins. She went on to work with cocoa powder and grape seed extract procyanidins at Virginia Tech where she received Bachelor’s degrees in Food Science & Technology and Human Nutrition, Foods, & Exercise Science. She graduated as the Outstanding Senior in the Food Science program. Upon graduation, Laura matriculated to the North Carolina State University to obtain her Master’s degree in Food Science. While in school, Laura also completed two product development internships at The J.M. Smucker Company and one at The Hershey Company, participated in the Danisco Product Development Competition, and served as a co-chair for the NCSU Food Science Club Product Development Committee. After completing her Masters, Laura will pursue a PhD at Virginia Tech. In her spare time, Laura teaches group fitness classes at local gyms. She is a certified group fitness instructor and personal trainer. She teaches everything from Pilates to high intensity interval training. Laura also enjoys reading, traveling, playing with her Shetland sheepdog, and competitive ballroom dancing. ii ACKNOWLEDGMENTS I would like to acknowledge the members of my committee, the members of the USDA MQHR unit, and the Sensory Service Center. Without their help, I would not have been able to complete my graduate work. iii TABLE OF CONTENTS LIST OF TABLES………………………………………………………………………….ix LIST OF FIGURES………………………………………………………………………...xii CHAPTER 1: LITERARY REVIEW………………………………………………………1 CASHEWS………………………………………………………………………………..2 Introduction……………………………………………………………………………………..2 History…………………………………………………………………………………………...3 Botany……………………………………………………………………………………………5 Cashew Varietals……………………………………………………………………………....6 Cashew Apples………………………………………………………………………………….6 Cashew Nut Shell Liquid………………………………………………………………………7 Cashew Testa………………………………………………………………………………...…8 Cashew Growth and Harvest………………………………………………………………...8 Cashew Nut Quality………………………………………………………………………….10 Cashew Processing…………………………………………………………………………..11 Chemical Composition……………………………………………………………………….13 Lipid…………………………………………………………………………………………….13 Phytosterols……………………………………………………………………………………15 Phospholipids………………………………………………………………………………....16 Carbohydrate………………………………………………………………………………….16 Protein………………………………………………………………………………………….18 Free Amino Acids and Flavor……………………………………………………………....20 Vitamins and Minerals……………………………………………………………………….22 Antioxidant Properties……………………………………………………………………….23 Nut Consumption and Human Health…………………………………………………...…26 LABORATORY METHODS……………………………………………………………28 iv High Performance Liquid Chromatography………………………………………………28 Gas Chromatography……………………………………………………………………..…30 Spectrophotometry……………………………………………………………………………31 Flavor Lexicon Development……………………………………………………………….32 Texture Lexicon Development………………………………………………………………34 Consumer Testing…………………………………………………………………………….35 REFERENCES…………………………………………………..………………………37 CHAPTER 2: CHEMICAL ANALYSIS OF THE CASHEW…………………………..62 ABSTRACT………………………………………………………………………..……63 INTRODUCTION………………………………………………………………..……...64 MATERIALS AND METHODS………………………………………………………..66 Determination of Moisture Content…………………………………………………….….66 Determination of Total Oil Content………………………………………………………..67 Determination of Tocopherols……………………………………………………………...67 Determination of Fatty Acid Profile………………………………………………...……..68 Determination of Phytosterol Content……………………………………………………..70 Determination of Phospholipid Content…………………………………………………..72 Determination of Carotenoid Content……………………………………………………..74 Determination of Vitamin K Content…………………………………………………..…..74 Determination of Sugar Profile……………………………………………………………..75 Determination of Total Nitrogen……………………………………………………………77 Determination of Total Protein……………………………………………………………..78 Determination of Total Amino Acids……………………………………………………….78 v Determination of Thiamin and Riboflavin Content………………………………….…..81 Determination of Niacin Content…………………………………………………….…….81 Determination of Cashew Mineral Content……………………………………………….83 Determination of Total Phenolics…………………………………………………………..83 Determination of Total Antioxidant Activity………………………………………………85 Determination of Oxygen Radical Absorbance Capacity……………………………….87 Determination of Procyanidin Content……………………………………………………88 Determination of Small Molecule Phenolics……………………………………………..89 Determination of Cashew Nut Shell Liquid Residue…………………………………….90 RESULTS AND DISCUSSION…………………………………………………………91 Moisture…………………………………………………………………………………….....91 Lipids in Cashews…………………………………………………………………………….93 Phytosterols……………………………………………………………………………………97 Phospholipids…………………………………………………………………………………98 Carbohydrate………………………………………………………………………………..101 Protein………………………………………………………………………………………..104 Amino Acid Profile………………………………………………………………………….106 Free Amino Acids…………………………………………………………………………...109 B Vitamins……………………………………………………………………………………112 Fat Soluble Vitamins………………………………………………………………………..119