DOCSLIB.ORG

Florida State University Libraries

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Florida State University Libraries Florida State University Libraries Electronic Theses, Treatises and Dissertations The Graduate School 2017 Neuromodulation of Mitral Cells by Serotonin and GLP-1 Neurons in the Olfactory Bulb and the Consequences of Gene Deletion of Kv1.3 Zhenbo Huang Follow this and additional works at the DigiNole: FSU's Digital Repository. For more information, please contact [email protected] FLORIDA STATE UNIVERSITY COLLEGE OF ARTS AND SCIENCES NEUROMODULATION OF MITRAL CELLS BY SEROTONIN AND GLP-1 NEURONS IN THE OLFACTORY BULB AND THE CONSEQUENCES OF GENE DELETION OF KV1.3 By ZHENBO HUANG A Dissertation submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Doctor of Philosophy 2017 Zhenbo Huang defended this dissertation on November 16, 2017. The members of the supervisory committee were: Debra Ann Fadool Professor Directing Dissertation Timothy M. Logan University Representative David M. Gilbert Committee Member Lisa C. Lyons Committee Member Zuoxin Wang Committee Member The Graduate School has verified and approved the above-named committee members, and certifies that the dissertation has been approved in accordance with university requirements. ii This work is dedicated to my parents, Shengding Huang and Heyin Tang, for their unconditional support throughout my life. Without them, I could not have achieved this. iii ACKNOWLEDGMENTS First, I would love to thank my mentor, Dr. Debra Ann Fadool, for her attention and guidance to various aspects of my Ph.D. training. With her hands-on teaching, I acquired solid patch-clamp technique. Her rigorous writing style has helped me to become a better writer. Her scientific think enabled me to do thorough science. She also serves as a role model for me in life. Her kindness and patience to everyone inspires me to do the same. I would also like to thank my Graduate Committee, Drs. David Gilbert, Lisa Lyons, Timothy Logan, and Zuoxin Wang for their insightful comments and suggestions to my research. I learned a lot from them. Thanks to post-docs, Dr. Nicolas Thiebaud and Dr. Erminia Fardone, and my fellow graduate students, Austin Schwartz, Brandon Chelette, Daniel Landi Conde, Dolly Al Koborssy, Genevieve Bell, and Kassandra Ferguson, and Louis Colling in the lab. They helped me both in scientific experiments and in daily life. With their help I made a relative easy and pleasant transition to live in a foreign country. Dr. James M. Fadool and his laboratory crew were also very helpful during my Ph.D. study. Last, but not least, I would like to thank all the faculty and staff in the Program in Neuroscience and in the Biological Science Department who helped me along the way. Their help made me feel at home and contributed to my achievements here at Florida State University. iv TABLE OF CONTENTS List of Tables ................................................................................................................................ vii List of Figures .............................................................................................................................. viii List of Abbreviations .......................................................................................................................x Abstract ......................................................................................................................................... xii 1. INTRODUCTION ......................................................................................................................1 1.1 Origin of Neuro-electricity and the Development Patch-clamp Technology .......................1 1.2 Introduction of Ion Channels and their Function ..................................................................5 1.3 Neuromodulation in the Olfactory Bulb ...............................................................................9 2. DIFFERENTIAL SEROTONERGIC MODULATION ACROSS THE MAIN AND ACCESSORY OLFACTORY BULBS ....................................................................................14 2.1 Introduction .........................................................................................................................14 2.2 Materials and Methods……………………………………………………………………16 2.3 Results. ................................................................................................................................21 2.4 Discussion ...........................................................................................................................36 3. THE MODULATION OF GLP-1 NEURONS IN THE OLFACTORY BULB ......................41 3.1 Introduction .........................................................................................................................41 3.2 Materials and Methods……………………………………………………………………43 3.3 Results. ................................................................................................................................49 3.4 Discussion ...........................................................................................................................55 4. OLFACTION AND ANXIETY IN KV1.3 KNOCK-OUT MICE: POSSIBLE APPLICATION IN ADHD.......................................................................................................60 4.1 Introduction .........................................................................................................................60 4.2 Materials and Methods……………………………………………………………………62 4.3 Results. ................................................................................................................................69 4.4 Discussion ...........................................................................................................................77 5. SUMMARY ..............................................................................................................................84 APPENDICES ...............................................................................................................................87 A. KV1.3 AND MITOCHONDRIA ...............................................................................................87 B. TESTING QUANTUM DOT-CONJUGATED DRUG ON BRAIN SLICE ...........................95 C. THE EFFECTS OF SHORT-TERM PRENATAL HIGH-FAT DIET ON OFFSPRINGS AND DAMS THEMSELVES ..................................................................................................96 D. ACUC APPROVAL LETTER ..................................................................................................99 v REFERENCES ............................................................................................................................100 BIOGRAPHIC SKETCH.............................................................................................................127 vi LIST OF TABLES 2.1 A comparison of intrinsic properties of mitral cells (MCs) in the main olfactory bulb (MOB) versus those in the accessory olfactory bulb (AOB) ................................................................21 3.1 Basic intrinsic properties of GLP-1 neurons in the olfactory bulb ..........................................50 C.1 Litter statistics comparing dams maintained on control- and high-fat diets ...........................98 vii LIST OF FIGURES 1.1 Basic model of the olfactory bulb neural network. ..................................................................11 2.1 Mitral cells in the main olfactory bulb (MOB MCs) largely exhibit an increase in action potential (AP) firing frequency in response to serotonin (5-HT) ............................................23 2.2 Quantitative analysis of the excitatory response of MOB MCs. .............................................24 2.3 Serotonin (5-HT) elicits direct excitation of MOB MCs. ........................................................26 2.4 5-HT-evoked excitation in MOB MCs is inhibited by 5-HT receptor antagonists that have subtype specificity. ..................................................................................................................27 2.5 Mitral cells in the accessory olfactory bulb (AOB MCs) predominantly exhibit a decrease in AP firing frequency in response to 5-HT ................................................................................28 2.6 AOB MCs are differentially inhibited by 5-HT, regardless of onset kinetics of the modulation. ..............................................................................................................................31 2.7 5-HT-evoked inhibition in AOB MCs responds differentially to gabazine. ...........................32 2.8 Slow-onset 5-HT modulation is sensitive to 5-HT2 receptor antagonists while rapid-onset is sensitive to 5-HT1 receptor antagonist in AOB MCs. ..............................................................34 2.9 Model of serotonergic modulation in two parallel processing pathways of the olfactory system. .....................................................................................................................................40 3.1 Representative current-clamp recording of a GLP-1 neuron. ..................................................50 3.2 Acetylcholine shows inhibitory effects on GLP-1 neurons. ....................................................51 3.3 Acetylcholine shows excitatory effects on GLP-1 neurons. ....................................................52 3.4 Cholecystokinin modulates the activity of GLP-1 neurons. ....................................................53 3.5 A subgroup of GLP-1 neurons responds
Load more

Recommended publications
  • Spring Mitre Clamp Set • Small This Is the Perfect Clamp Set When You Need to Hold ‘C’ Clamp to Holding a Mitre While the Glue Sets
    We appreciate the opportunity to supply you with quality woodworking supplies at great prices. 4-Way Clamp Ideal for panels, doors, and more! The lever action of the clamp heads applies even pressure to the face of the lumber while applying clamping pressure. • No one-sided pressure. • No buckling. • No misalignment of lumber at the glue joint. A perfect joint every time. Clamp Anything! Clamp any shape! 13’ Strap Clamp The 13 foot strap wraps up inside the clamp body. Simply turn the tensioning knob to tighten or lift to release. Very handy! Includes: • 7 corner brackets Spring Mitre Clamp Set • Small This is the perfect clamp set when you need to hold ‘C’ clamp to holding a mitre while the glue sets. the body to bench top The ends of the springs are just sharp enough to dig into the wood and hold while you set the rest of the BlackJackCompany.com joints and wait for the glue to dry. Very handy! BlackJackTM Hardware Woodworking Tools • casters [email protected] • drawer slides Part Wholesale 100 Commissioner St E,PO Box 160 • euro hinges Number Product Each Embro, Ontario, Canada N0J 1J0 • fasteners 10102 13’ Strap Clamp 12.97 Phone (519) 475-4947 • legs Toll Free 1-800-387-5716 • multi-media 10103 4-Way Clamp 23.95 Fax (519) 475-4590 • swivels 10104 4-Way Clamp Blocks 6.57 Fax 1-800-561-3045 Dust Collection 16231 2-1/2” Springs - 8 pkg 7.77 • fittings Tools Supplies • adapters 16232 3-3/8” Springs - 8 pkg 7.77 • clamping • abrasives • collectors • drilling • drawer slides 16233 4-1/8” Springs - 8 pkg 8.37 • fittings • routering • joining • hose 16234 5” Springs - 8 pkg 8.37 • measuring • mats • tablesaw tools • storage ...and so 17522 Spring Mitre Clamp Set in Case 23.97 • safety • switches much more! Authorized BlackJack Dealers Confidential Price List How To Order Pick-ups: Order pick-ups by dealers can Prices: Prices are listed in Canadian funds, • Call toll free 1-800-387-5716 • 519-475-4947 be arranged with customer service.
    [Show full text]
  • STONE FAB CATALOG 2018-V1 Your Stone, Tile and Concrete Supply Experts Since 1971
    STONE FAB CATALOG 2018-V1 Your stone, tile and concrete supply experts since 1971 Component Bonder Macaron Pads 5” Cordless 18V Angle Quick-Change magnetic Stone Bonder Grinder backup pad WWW.GRANQUARTZ.CA see page 157 & 162 see page 41 see page 203 see page 34 STONE FAB CATALOG, 2018-V1 Dear valued customer, I am proud to present to you our new 2018 Stone Fab Catalog. As we strive to bring you all the latest and most innovative solution for your production year after year, our goal is to find tools, supplies and services for you to be as efficient as possible in the safest way possible. We are working on several exciting projects that will bring you advanced solutions for your day-to-day business. These projects are at different stages of development and we will bring them to market when the time is right to properly offer them. Rest assured, our priority is to bring these benefits as soon as possible. We here at GranQuartz Canada, have been working hard on an internal project for several months now that will continue till mid 2018. We are implementing a new state of the art ERP system. All our dedicated employees are involved in different aspects of this project, such as drawing out our internal processes, training programs and all this while doing their regular jobs to keep the same service level to you. This is a very big project that demands a lot of extra work and planning. We planned on a one year time frame to set up all of the parameters and we plan to go live in June 2018 and be able to reap the benefits of this new system right from day one.
    [Show full text]
  • Apprenticeship Curriculum Standard Pattern Maker Level 2 443A 2004
    Apprenticeship Curriculum Standard Pattern Maker Level 2 443A 2004 PATTERN MAKER Level 2 Hours Disclaimer: It is agreed that Training Delivery Agents (TDAs) may need to make slight adjustments (with cause) according to particular apprentice needs and may deviate from the unit sequencing and the prescribed Practical: and theoretical hours shown within the standard. However, all TDAs will comply with the hours at the reportable subject level. Please Note: Apprenticeship Training and Curriculum Standards were developed by the Ministry of Training, Colleges and Universities (MTCU). As of April 8th, 2013, the Ontario College of Trades (College) has become responsible for the development and maintenance of these standards. The College is carrying over existing standards without any changes. However, because the Apprenticeship Training and Curriculum Standards documents were developed under either the Trades Qualification and Apprenticeship Act (TQAA) or the Apprenticeship and Certification Act, 1998 (ACA), the definitions contained in these documents may no longer be accurate and may not be reflective of the Ontario College of Trades and Apprenticeship Act, 2009 (OCTAA) as the new trades legislation in the province. The College will update these definitions in the future. Meanwhile, please refer to the College’s website (www.collegeoftrades.ca) for the most accurate and up-to-date information about the College. For information on OCTAA and its regulations, please visit: www.collegeoftrades.ca/about/legislation-and-regulations. © Ontario College of Trades PATTERN MAKER Level 2 Table of Contents Introduction .......................................................................................................... 3 Summary of Total Program In-School Training Hours .......................................... 6 76.0 Applied Trade Calculations ......................................................................... 7 77.0 Engineering Drawings, Charts and Tables ..............................................
    [Show full text]
  • STONE FAB CATALOG 2020-V1 Your Stone, Tile and Concrete Tool Supply Experts Since 1971
    STONE FAB CATALOG 2020-V1 Your stone, tile and concrete tool supply experts since 1971 C • Less risk of damaging tools • Increase tools life • Quicker set up • Better accuracy • Increase productivity • Increase parameters • Overall better quality © Grabo Nemo Portable Aardwolf Thin Material ® Electric Vacuum Cup Diarex VB Core Drills Slab Gripper Alpha AIR-830/850 see page 166 see page 78 & 81 see page 156 see page 231 STONE FAB CATALOG, 2020-V1 WWW.GRANQUARTZ.CA Dear valued customers, It’s just now been a year since we’ve implemented our new Enterprise Resource Planning (ERP) System. Despite the amount of preparation involved in this transition, it was a rough period to adapt to our new system and still offer the same level of service. Even after the change of system, we faced many unpredictable issues necessitating quick and efficient decisions altering the processes that were previously agreed on. Throughout all these challenges, our team worked together to ensure that we continued to offer the best possible service to you. We thank you for your patience and understanding as we sorted out those issues. Now that we have a solid foundation, we plan to work on numerous other projects that will connect with our ERP database to offer better and quicker solutions to you. During the previous year we have begun offering what we call the “Zoller service” for calibrating CNC tools so that you can save hours when you set up new tools or redressed tools on your CNC machine. If you haven’t heard of this service please ask your local sales and service representative or our customer service agents for more information.
    [Show full text]
  • 10-Inch Tilting Arbor
    OPERATING INSTRUCTIONS AND PARTS LIST FOR C |smqE 10-inch Tilting Arbor , , Model Number 113.27521 The above Model Number will be found on a plate attached to your saw, at the back, near the bottom of the base. Always men- tion the Model Number when communicating with us regarding your saw or when ordering parts. Instructions for Ordering Parts All parts listed herein must be ordered through a Sears retail store or mail order house. Parts are shipped prepaid. When ordering repair parts, always give the following information: 1. The part number. 2. The part name. 3. The model number, proper parts service. We suggest you keep it with other valuable papers.This list is valuable. It will assure your being able to obtain ........... ii i • i i i CHECK ARBOR NUT- Remove two screws (B, fig. 1) AB LE F H O M and lift out the insert (A, fig. 1). Check tightness of [ saw arbor nut (part 6362, fig. 5), / using wrench (M, fig. 1). Re- ""--_'I place insert and screws. ! ALIGN SAW BLADE WITH TABLE GROOVES- Using ele- vation hand wheel (A, fig. 2), set saw blade for deepest cut. Make pencil mark on tooth that is just above table top at front of blade. Measure distance from this tooth point to right-hand table groove (L, fig. 1). Rotate blade by hand to place this same tooth just above table top at rear then again T D J g measure distance from tooth point to right-hand groove. The two measurements must be exactly equal.
    [Show full text]
  • AEG Powertools Catal
    POWEPOWERING PROFEOFESSIONONALSALS SINCESINCE 1898 CATALOGUE 2010/11 www.aeg-powertools.com AEG POWERTOOLS POWERING PROFESSIONALS SINCE 1898 AEG POWERTOOLS promises to draw upon its heritage and deliver exciting, high performance, innovative working solutions for the professional tradesmen of today and the future. AEG HISTORY Since being founded, AEG POWERTOOLS has been leading the way in new product development. By introducing the first transportable drilling machines in 1898, portable powertools were born. Over 100 years later AEG are still offering the professional user innovative, powerful solutions… PROUD PAST... EXCITING FUTURE... RES FEATU KEY FIX-TEC system Keyless Safety Guard Impact Driver For changing accessories in Safety guard adjustment without For heavy duty screwdriving seconds. the use of a tool. applications. Anti-Vibration-System Soft Hammer Mode HEX 1/4” Significantly reduces vibration Reduces blow energy for increased control. 1/4” quick release hex fitment. by up to 50%. Safety Clutch 2-Speed Gear Pendulum Action Protects user and machine if a drill bit 2-speed gearbox, for optimum speed to For a faster, more aggressive cut. should jam. suit application. Dust Extraction Drill Only Kitbox Integrated dust extraction for a clean Single mode - rotary drilling. Heavy duty kitbox. working environment. Electronics Drill And Hammer Drill Kitbag With variable speed pre-selection, softstart, 2 mode - rotary and rotary hammer drilling. Heavy duty kitbag. constant speed and overload protection. Chisel And Hammer Drill L.E.D. Crosscut Capacity 2 mode - rotary hammer drill with chipping/ For workplace illumination. Maximum crosscut capacity of saw. demolition function. Combi Hammer Metal Chuck Mitre Saw Blade Laser 3 mode - rotary and rotary hammer drilling For enhanced durability.
    [Show full text]
  • Second Major Fire Ts Asbury Park; Ss at $1 Million by CHARLES A
    Second Major Fire ts Asbury Park; ss at $1 Million By CHARLES A. JOHNSTON Asbury pavilion was the oldest so-called perma- nent building on the walk. ASBURY PARK—The second of two apparent- Nine firemen, of more than 250 from Asbury ly matching fires in five weeks yesterday destroyed Park and seven surrounding communities who bat- a long section of city boardwalk and a beachfront tled the three-hour blaze, suffered burns, cuts and two-story brick city pavilion. smoke poisoning. All were treated at Fitkin Hos- In carbon-copy fashion to the fire of Aug. 6 pital, Neptune, and were released. which wrecked the Sunset Avenue Pavilion, and About 50 customers and store personnel es- 550 feet of boardwalk, at the north end of the caped unhurt. beach, fire whipped through the Asbury Avenue More than 5,000 people flocked to the scene Pavilion arid 850 feet of promenade. just north of the Casino which arcs the boardwalk at the entrance to Ocean Grove. The fire broke Smoke was so dense on both occasions that out at 12:15 p.m. roaring, leaping flames couldn't be seen at times. Though the cause of either boardwalk fire is AT THE END — Aerial phpto reveals Asbury Park boardwalk and pavilion loss after the.smoke has cleared anci City Manager Kendall H. Lee, who had esti- as yet undetermined, Mr. Lee and Fire Chief Mi- flames have burned out. In background, across from gutted area, it Empress Motel which was evacuated and mated the Sunset loss at.$800,000, said the As- chael De Sarno agreed both started in the same Wet down by firemen as precautionary measure.
    [Show full text]
  • For Industrial, Commercial and Retail Applications
    Contamination removal systems Full range of products and services to protect all process lines and products from foreign body contamination. ■ Magnetic separators ■ Vibratory sieves ■ Metal detectors ■ Site audits Magnetic Products For industrial, commercial Manufactured under an ISO 9001 registered quality and retail applications management system. www.eclipsetoolsinc.com FM31278 A world leader in magnetic technology A world leader in magnetic technology 2 A world leader in magnetic technology Eclipse Magnetics is at the forefront A worldof leader developments in magnetic in magnetic technology materials and design. With over 100 years experience, we have a proven track record for supplying quality products and providing total customer support to some of the Contents leading names in industry. Magnet Materials Alnico 1 Cylindrical Bar Magnets Rectangular Bar Magnets Eclipse Tools North America Inc., is a Button Magnets wholly owned subsidiary of Spear & Minor Magnets Jackson PLC, and is responsible for Pocket Magnets the sales, distribution and customer service of our group's magnetics, Alnico 2 Power Magnets hand tools and metrology products Deep Pots under the professional brands- Shallow Pots Eclipse Magnetics, Neill Tools, Spear & Jackson, Moore & Wright - Ceramic 3 throughout North America. Channel Magnets Shallow Pots Shallow Pots - Threaded Shallow Pots - Countersunk You've used our products... Heavy Duty Shallow Pots Magnetic Hooks There's a very good chance that today you've used something that either Neodymium 4 contains or was manufactured with the Disc Magnets aid of an Eclipse Magnetics product. Nickel Plated Pots Bi-Pole Deep Pots – Threaded Our range includes over 20,000 Pot Magnets magnets and magnetic products which Neodymium 5 are used in most industries, including Shallow Pots - Loop automotive, steel, food, environmental, Shallow Pots – Bolt communications, petrochemical, and engineering as well as in commercial, Samarium Cobalt 5 Deep Pots office and retail premises.
    [Show full text]
  • Carpentry and Joinery
    ’ dtbe £uilb er b tufimt s s wim. CARP ENTRY AND J O I NERY . A X - BO O K FO R R C HI C N G IN R TE T A TE TS , E EE S , - S R VE Y O R S A N D R F M N . U , C A TS E FULL Y I LLUS T R A T E D A N D W R ITTE N BY B A R F FLE T C HE R N I ST E . , A ssociat e of the R oyal I nstitu te of B r iti sh A r chitects Vi ce - P r e sid ent ' o the A r ch i te ctu r al A ssocia tion oi nt A u thor A Histor o f j , y f A r chite ctu r e Lectu r e r on A r chitectu r e and B u ild ing ’ ’ C onstr uction and D ir e ctor o the Stu d io i n s , f , g ine i n ar e ntr d . ate xam r C C oll . , Lon L E p y and joine ry to the C ity and Gu ild s n nstitu te of Lond o I , 63 c A N D H P HI LL I P S FLE T C HE R . , A ssociate of the R oyal I nstitu te of B r itish A r chite cts ; Fe llow of the ’ ’ S u rveyor s I nstitu tion D i r ector of the C ity C ompanies T r ad es T r aini n S chool Gr e at T itch eld Str e e t W.
    [Show full text]
  • Robert Larson Company 
    Robert Larson Company 1 Chisel Sets Bevel Edge Chisels Boxed Chisel Sets Wooden Handles Two different sets of high quality Two Cherries bevel edge chisels with These chisels are made of the highest quality German steel. Carefully made, pol- hardwood handles. The set of six ished all-around. One of the finest tools made in the world! Ten to fourteen inches chisels consists of sizes 6-10-12-16- in overall length. Choose between a traditional and well proportioned wooden Set of four and a set of six wooden 20-26mm, the set of four consists of handle, or nearly unbreakable cellulose acetate plastic handle. A number of sets are handled bevel edge chisels in a fitted 10-16-20-26mm. available which include the most important sizes. Inch sizing is approximate. wooden box. Keeps your chisels pro- tected in storage and ready to use. Set 500-1562 ..................................... Set of Six 500-1564 ...................................Set of Four Carefully forged blades are skillfully heat treated in an immersion bath method of six contains 6-10-12-16-20-26mm to achieve a Rockwell hardness of 61o C. This means that sharpening the chisels chisels. Set of four contains 10-16-20- may take a few extra minutes, but they will hold an edge significantly longer than 26mm chisels. almost any other chisels! After heat treating, the blades are polished to a rich finish 500-1559 .........Set of Four in Wooden Box 500-1561 ...........Set of Six in Wooden Box that makes them a joy to use. Fitting appropriate handles is the final step in produc- Chisel Sets ing these highest quality German chisels.
    [Show full text]
  • No. 商標 画像 出願日 登録日 出願人／権利者 代理人 区分 指定商品・役務 ステータス 1 Tokyo （東京） 34 Korek Api, Flint, Gas Lighter (Lighters)
    別紙1 インドネシア検索結果 No. 商標 画像 出願日 登録日 出願人／権利者 代理人 区分 指定商品・役務 ステータス 1 Tokyo （東京） 34 Korek Api, Flint, Gas Lighter (Lighters) 1-1 Tokyo なし 2007/8/31 PT PAKABAJA (Indonesia) 34 REGISTERED 12 Shock Absorber (Shockbacker), Suspension Arm (suspension), Wiper Blade (Fan Glass) 1-2 TOKYO N KYOTO なし 2010/2/8 DAVID MEIDIANTO (Indonesia) 12 REGISTERED 21 Equipment Requirements Not Eat Of Precious Metal, Needs Bathrooms, Coral Flower Bathroom, Attached Bath Container Equipment, Kitchen Equipment Instead Of Precious Metals, Bottle-Bottles, Cosmetic Equipment. 1-3 TOKYO 1 2010/8/20 HASAN (Indonesia) 21 REGISTERED Shirt; jacket; tie; footwear; outerwear; underwear; all barang2 mentioned above are included in 1-4 Tokyo Bay 2011/5/25 2013/9/10 FM Creative Pte Ltd (SG) 25 REGISTERED 02 All types of paints, wall paints, asbestos paints, aluminum paint, lacquer paint, ceramic paint, paint for the base vehicle chassis, paint thinners, paint thickener, paint materials for leather and wood,??? paint shiny platinum for ceramics, paint base, fireproof paint, epoxy 1-5 Tokyo なし 2011/6/16 RICHARD LITYO (Indonesia) 2 paint, paint an email, ship paint, paint for metal, copal varnish, lacquer solvent, varnish to REGISTERED give color bronze, japanese black lacquer (paint bitumen) 04 Grease Lubricant, Grease For Industry, Fat-Fat, Oil Lubricant. 1-6 Tokyo 2011/12/14 SANDY WIJAYA (Indonesia) 4 REGISTERED 09 ACCU PT SUMBER REJEKI LANCAR BATTERY 1-7 Tokyo 2012/7/30 9 REGISTERED (Indonesia) The tools are moved by hand, namely: the grinding wheel, wheel-shaped stone cutter (cutting wheel), stone grinding of aluminum oxide, diamond glass cutters, emery / grindstones, grinding wheel 1-8 TOKYO 2014/10/27 PT WHITE DOVE INDONESIA (ID) 8 shape, grinding sharpener, PENDING plain stone for geranit.
    [Show full text]
  • Clamps & Vices
    Clamps & Vices G Clamps Deep Axminster Trade Clamps Throat The clamps that trade use G Clamp Every workshop ought to What are our clamps made from and why? have a few deep throat Within this section, clamps come in a variety of materials. G clamps, as they’re Steel in one form or another predominates. So why use one invaluable for reaching type of steel rather than another? Clamps in particular exert G Clamp into those slightly more considerable force. The clamp has to withstand these forces Axminster G clamps are drop forged from quality inaccessible areas that are out of reach to without breaking, bending or permanently deforming. A clamp steel with extra webs at the stress points. Each features a strong standard clamps. Lifetime guarantee. that fails under load or ends up distorted is no use to anyone. rolled thread steel screw, live clamping pad and a tommy bar • 50 x 90mm - 190kg max clamping pressure Cold Drawn Steel with which to apply pressure. Lifetime guarantee. • 25 x 72mm – 250kg max clamping pressure • 75 x 120mm – 350kg max clamping pressure This is generally used when referring to the bars of sash and Inc.vat Ex.vat Code F clamps. Hot rolled steel bar would be cheaper and easier to • 50 x 30mm – 270kg max clamping pressure 50 X 90mm £3.46 £2.88 505519 use. However, steel bar reduced at room temperature by pulling • 75 x 50mm – 290kg max clamping pressure 75 X 120mm £5.76 £4.80 505520 (drawn) through an oil-lubricated die is superior in many ways.
    [Show full text]