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Katalog 2015 Cover Paul Lin *Hinweis Förderung.Indd
Product List 2015 WE LIVE SERVICE Certificates quartett owns two productions sites that are certified according to EN ISO 9001:2008 Quality management systems - Requirements EN ISO 13485:2012 + AC:2012 Medical devices - Quality management systems - Requirements for regulatory purposes GMP Conformity Our quality management guarantees products of highest quality! 2 Foreword to the quartett product list 2015 quartett Immunodiagnostika, Biotechnologie + Kosmetik Vertriebs GmbH welcomes you as one of our new business partners as well as all of our previous loyal clients. You are now member of quartett´s worldwide customers. First of all we would like to introduce ourselves to you. Founded as a family-run company in 1986, quartett ensures for more than a quarter of a century consistent quality of products. Service and support of our valued customers are our daily businesses. And we will continue! In the end 80´s quartett offered radioimmunoassay and enzyme immunoassay kits from different manufacturers in the USA. In the beginning 90´s the company changed its strategy from offering products for routine diagnostic to the increasing field of research and development. Setting up a production plant in 1997 and a second one in 2011 supported this decision. The company specialized its product profile in the field of manufacturing synthetic peptides for antibody production, peptides such as protease inhibitors, biochemical reagents and products for histology, cytology and immunohistology. All products are exclusively manufactured in Germany without outsourcing any production step. Nowadays, we expand into all other diagnostic and research fields and supply our customers in universities, government institutes, pharmaceutical and biotechnological companies, hospitals, and private doctor offices. -
Datasheet BA3564-2 Anti-FES Antibody
Product datasheet Anti-FES Antibody Catalog Number: BA3564-2 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-FES Antibody Gene Name FES Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human FES(808-822aa STIYQELQSIRKRHR). Purification Immunogen affinity purified. Observed MW Dilution Ratios Western blot: 1:500-2000 Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information FES(feline sarcoma oncogene) is an enzyme that in humans is encoded by the FES gene, also known as Proto-oncogene tyrosine-protein kinase Fes/Fps, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, Proto-oncogene c-Fes, Proto-oncogene c-Fps, p93c-fes c-fes/fps protein, FPS, Oncogene FES, feline sarcoma virus, FPS. This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. Non-onc intervening sequences were present in the human counterpart. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of°Cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia, but it is also involved in normal hematopoiesis. A truncated transcript has been identified that is generated utilizing a start site in one of the far downstream exons but a protein product associated with this transcript has not been identified. -
Supplementary Table 1. in Vitro Side Effect Profiling Study for LDN/OSU-0212320. Neurotransmitter Related Steroids
Supplementary Table 1. In vitro side effect profiling study for LDN/OSU-0212320. Percent Inhibition Receptor 10 µM Neurotransmitter Related Adenosine, Non-selective 7.29% Adrenergic, Alpha 1, Non-selective 24.98% Adrenergic, Alpha 2, Non-selective 27.18% Adrenergic, Beta, Non-selective -20.94% Dopamine Transporter 8.69% Dopamine, D1 (h) 8.48% Dopamine, D2s (h) 4.06% GABA A, Agonist Site -16.15% GABA A, BDZ, alpha 1 site 12.73% GABA-B 13.60% Glutamate, AMPA Site (Ionotropic) 12.06% Glutamate, Kainate Site (Ionotropic) -1.03% Glutamate, NMDA Agonist Site (Ionotropic) 0.12% Glutamate, NMDA, Glycine (Stry-insens Site) 9.84% (Ionotropic) Glycine, Strychnine-sensitive 0.99% Histamine, H1 -5.54% Histamine, H2 16.54% Histamine, H3 4.80% Melatonin, Non-selective -5.54% Muscarinic, M1 (hr) -1.88% Muscarinic, M2 (h) 0.82% Muscarinic, Non-selective, Central 29.04% Muscarinic, Non-selective, Peripheral 0.29% Nicotinic, Neuronal (-BnTx insensitive) 7.85% Norepinephrine Transporter 2.87% Opioid, Non-selective -0.09% Opioid, Orphanin, ORL1 (h) 11.55% Serotonin Transporter -3.02% Serotonin, Non-selective 26.33% Sigma, Non-Selective 10.19% Steroids Estrogen 11.16% 1 Percent Inhibition Receptor 10 µM Testosterone (cytosolic) (h) 12.50% Ion Channels Calcium Channel, Type L (Dihydropyridine Site) 43.18% Calcium Channel, Type N 4.15% Potassium Channel, ATP-Sensitive -4.05% Potassium Channel, Ca2+ Act., VI 17.80% Potassium Channel, I(Kr) (hERG) (h) -6.44% Sodium, Site 2 -0.39% Second Messengers Nitric Oxide, NOS (Neuronal-Binding) -17.09% Prostaglandins Leukotriene, -
Anti-Eph Receptor A1 Antibody Catalog # ABO10887
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Anti-Eph Receptor A1 Antibody Catalog # ABO10887 Specification Anti-Eph Receptor A1 Antibody - Product Information Application WB, IHC Primary Accession P21709 Host Rabbit Reactivity Human, Mouse, Rat Clonality Polyclonal Format Lyophilized Description Rabbit IgG polyclonal antibody for Ephrin type-A receptor 1(EPHA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Reconstitution Anti-Eph receptor A1 antibody, ABO10887, Add 0.2ml of distilled water will yield a Western blottingLane 1: Rat Liver Tissue concentration of 500ug/ml. LysateLane 2: Rat Lung Tissue LysateLane 3: Rat Intestine Tissue LysateLane 4: Rat Ovary Tissue LysateLane 5: U87 Cell LysateLane 6: Anti-Eph Receptor A1 Antibody - Additional A549 Cell LysateLane 7: COLO320 Cell Information LysateLane 8: SW620 Cell LysateLane 9: HELA Cell Lysate Gene ID 2041 Other Names Ephrin type-A receptor 1, hEpha1, 2.7.10.1, EPH tyrosine kinase, EPH tyrosine kinase 1, Erythropoietin-producing hepatoma receptor, Tyrosine-protein kinase receptor EPH, EPHA1, EPH, EPHT, EPHT1 Calculated MW 108127 MW KDa Application Details Immunohistochemistry(Paraffin-embedded Anti-Eph receptor A1 antibody, ABO10887, Section), 0.5-1 µg/ml, Human, Rat, Mouse, IHC(P)IHC(P): Rat Brain Tissue By Heat<br>Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse<br> Subcellular Localization Cell membrane ; Single-pass type I membrane protein . Tissue Specificity Overexpressed in several carcinomas. Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Protein Name Ephrin type-A receptor 1(hEpha1) Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. -
Protein Tyrosine Kinases: Their Roles and Their Targeting in Leukemia
cancers Review Protein Tyrosine Kinases: Their Roles and Their Targeting in Leukemia Kalpana K. Bhanumathy 1,*, Amrutha Balagopal 1, Frederick S. Vizeacoumar 2 , Franco J. Vizeacoumar 1,3, Andrew Freywald 2 and Vincenzo Giambra 4,* 1 Division of Oncology, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada; [email protected] (A.B.); [email protected] (F.J.V.) 2 Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada; [email protected] (F.S.V.); [email protected] (A.F.) 3 Cancer Research Department, Saskatchewan Cancer Agency, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada 4 Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies (ISBReMIT), Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, FG, Italy * Correspondence: [email protected] (K.K.B.); [email protected] (V.G.); Tel.: +1-(306)-716-7456 (K.K.B.); +39-0882-416574 (V.G.) Simple Summary: Protein phosphorylation is a key regulatory mechanism that controls a wide variety of cellular responses. This process is catalysed by the members of the protein kinase su- perfamily that are classified into two main families based on their ability to phosphorylate either tyrosine or serine and threonine residues in their substrates. Massive research efforts have been invested in dissecting the functions of tyrosine kinases, revealing their importance in the initiation and progression of human malignancies. Based on these investigations, numerous tyrosine kinase inhibitors have been included in clinical protocols and proved to be effective in targeted therapies for various haematological malignancies. -
FES Rabbit Polyclonal Antibody – TA590713 | Origene
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA590713 FES Rabbit Polyclonal Antibody Product data: Product Type: Primary Antibodies Applications: ELISA, WB Recommended Dilution: WB 1:20000, ELISA 1:100-1:2000 Reactivity: Human Host: Rabbit Isotype: IgG Clonality: Polyclonal Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein. Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0 Concentration: 0.83mg/ml Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Gene Name: FES proto-oncogene, tyrosine kinase Database Link: NP_001996 Entrez Gene 2242 Human P07332 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 3 FES Rabbit Polyclonal Antibody – TA590713 Background: FES (feline sarcoma oncogene) and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases. FES and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains. FES was originally identified as an oncogene from avian and feline retroviruses. -
Protein Kinase
Flexi® ORF Clone www.promega.co.jp/flexiclone/ Protein Kinase 355個 （2012/4/1 現在） 遺伝子シンボル 説明 FXC_PID 価格 FHC_PID 価格 AAK1 AP2 associated kinase 1 FXC00722 ¥40,000 FHC00722 ¥50,000 AATK apoptosis-associated tyrosine kinase FXC04004 ¥40,000 FHC04004M ¥60,000 ABL1 c-abl oncogene 1, receptor tyrosine kinase FXC01406 ¥40,000 FHC01406 ¥50,000 ACTR2 ARP2 actin-related protein 2 homolog (yeast) FXC02146 ¥40,000 FHC02146 ¥50,000 Activin receptor type-1 Precursor (EC 2.7.11.30)(Activin receptor type I)(ACTR-I)(Serine/threonine-protein kinase ACVR1 FXC03971 ¥40,000 FHC03971 ¥50,000 receptor R1)(SKR1)(Activin receptor-like kinase 2)(ALK- 2)(TGF-B superfamily receptor type I)(TSR-I) ACVR1B activin A receptor, type IB FXC01408 ¥40,000 FHC01408 ¥50,000 ACVR1C activin A receptor, type IC FXC09109M ¥50,000 FHC09109 ¥50,000 ACVRL1 activin A receptor type II-like 1, transcript variant 1 FXC28135M ¥50,000 FHC28135 ¥50,000 ADRBK1 adrenergic, beta, receptor kinase 1 FXC09648M ¥50,000 FHC09648 ¥50,000 ADRBK2 adrenergic, beta, receptor kinase 2 FXC27231M ¥50,000 FHC27231 ¥50,000 AKT1 v-akt murine thymoma viral oncogene homolog 1 FXC02215 ¥40,000 FHC02215 ¥50,000 AKT2 v-akt murine thymoma viral oncogene homolog 2 FXC26633 ¥40,000 FHC26633 ¥50,000 v-akt murine thymoma viral oncogene homolog 3 (protein AKT3 FXC02812 ¥40,000 FHC02812 ¥50,000 kinase B, gamma) ALK anaplastic lymphoma receptor tyrosine kinase FXC00337 ¥40,000 FHC00337 ¥50,000 A-Raf proto-oncogene serine/threonine-protein kinase (EC ARAF FXC08066 ¥40,000 FHC08066 ¥50,000 2.7.11.1)(A-raf-1)(Proto-oncogene -
Supporting Information
Supporting Information Noyes et al. 10.1073/pnas.1013486108 Table S1. Abbreviations Gene name Description ADRB3 Adrenergic, β-3-, receptor AGPAT6 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta) ARHGAP15 Rho GTPase activating protein 15 BRAF v-raf murine sarcoma viral oncogene homolog B1 CAPN2 Calpain 2, (m/II) large subunit CASP2 Caspase 2, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CD14 CD14 molecule CD28 CD28 molecule CDKN2D Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) CHRM2 Cholinergic receptor, muscarinic 2 CHRM2 Cholinergic receptor, muscarinic 2 CLDN23 Claudin 23 CTLA4 Cytotoxic t lymphocyte-associated protein 4 DGKI Diacylglycerol kinase, iota DKK4 Dickkopf homolog 4 (xenopus laevis) DUSP10 Dual-specificity phosphatase 10 DUSP4 Dual-specificity phosphatase 4 ECSIT ECSIT homolog (Drosophila) ECSIT ECSIT homolog (Drosophila) EPHA1 EPH receptor A1 EPHB6 EPH receptor B6 EPOR Erythropoietin receptor FCER2 Fc fragment of IgE, low affinity II, receptor for (CD23) FGF20 Fibroblast growth factor 20 FGFR1 Fibroblast growth factor receptor 1 GNA14 Guanine nucleotide binding protein (G protein), alpha 14 GNAQ Guanine nucleotide binding protein (G protein), q polypeptide GSTK1 GST kappa 1 ICAM1 Intercellular adhesion molecule 1 ICAM3 Intercellular adhesion molecule 3 ICOS Inducible T-cell costimulator IDH1 Isocitrate dehydrogenase 1 (nadp+), soluble IKBKB Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta INSR Insulin -
Supplementary Data
Supplementary Figure 1 Supplementary Figure 2 CCR-10-3244.R1 Supplementary Figure Legends Supplementary Figure 1. B-Myb is overexpressed in primary AML blasts and B-CLL cells. Baseline B-Myb mRNA levels were determined by quantitative RT-PCR, after normalization to the level of housekeeping gene, in primary B-CLL (n=10) and AML (n=5) patient samples, and in normal CD19+ (n=5) and CD34+ (n=4) cell preparations. Each sample was determined in triplicate. Horizontal bars are median, upper and lower edges of box are 75th and 25th percentiles, lines extending from box are 10th and 90th percentiles. Supplementary Figure 2. Cytotoxicity by Nutlin-3 and Chlorambucil used alone or in combination in leukemic cells. The p53wild-type EHEB and SKW6.4 cells lines, and the p53mutated BJAB cell line were exposed to Nutlin-3 or Chlorambucil used either alone or in combination. (Nutl.+Chlor.). In A, upon treatment with Nutlin-3 or Chlorambucil, used either alone (both at 10 μM) or in combination (Nutl.+Chlor.), induction of apoptosis was quantitatively evaluated by Annexin V/PI staining, while E2F1 and pRb protein levels were analyzed by Western blot. Tubulin staining is shown as loading control. The average combination index (CI) values (analyzed by the method of Chou and Talalay) for effects of Chlorambucil+Nutlin-3 on cell viability are shown. ED indicates effect dose. In B, levels of B-Myb and E2F1 mRNA were analyzed by quantitative RT- PCR. Results are expressed as fold of B-Myb and E2F1 modulation in cells treated for 24 hours as indicated, with respect to the control untreated cultures set to 1 (hatched line). -
Dema and Faust Et Al., Suppl. Material 2020.02.03
Supplementary Materials Cyclin-dependent kinase 18 controls trafficking of aquaporin-2 and its abundance through ubiquitin ligase STUB1, which functions as an AKAP Dema Alessandro1,2¶, Dörte Faust1¶, Katina Lazarow3, Marc Wippich3, Martin Neuenschwander3, Kerstin Zühlke1, Andrea Geelhaar1, Tamara Pallien1, Eileen Hallscheidt1, Jenny Eichhorst3, Burkhard Wiesner3, Hana Černecká1, Oliver Popp1, Philipp Mertins1, Gunnar Dittmar1, Jens Peter von Kries3, Enno Klussmann1,4* ¶These authors contributed equally to this work 1Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert- Rössle-Strasse 10, 13125 Berlin, Germany 2current address: University of California, San Francisco, 513 Parnassus Avenue, CA 94122 USA 3Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Strasse 10, 13125 Berlin, Germany 4DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Oudenarder Strasse 16, 13347 Berlin, Germany *Corresponding author Enno Klussmann Max Delbrück Center for Molecular Medicine Berlin in the Helmholtz Association (MDC) Robert-Rössle-Str. 10, 13125 Berlin Germany Tel. +49-30-9406 2596 FAX +49-30-9406 2593 E-mail: [email protected] 1 Content 1. CELL-BASED SCREENING BY AUTOMATED IMMUNOFLUORESCENCE MICROSCOPY 3 1.1 Screening plates 3 1.2 Image analysis using CellProfiler 17 1.4 Identification of siRNA affecting cell viability 18 1.7 Hits 18 2. SUPPLEMENTARY TABLE S4, FIGURES S2-S4 20 2 1. Cell-based screening by automated immunofluorescence microscopy 1.1 Screening plates Table S1. Genes targeted with the Mouse Protein Kinases siRNA sub-library. Genes are sorted by plate and well. Accessions refer to National Center for Biotechnology Information (NCBI, BLA) entries. The siRNAs were arranged on three 384-well microtitre platres. -
Decreased Eph Receptor‑A1 Expression Is Related to Grade in Ovarian Serous Carcinoma
MOLECULAR MEDICINE REPORTS 17: 5409-5415, 2018 Decreased Eph receptor‑A1 expression is related to grade in ovarian serous carcinoma YUNFENG JIN1, YI ZOU1, LINLING WAN1, MINGMING LU1, YA LIU2, GUOQIN HUANG3, JIANDONG WANG4 and QINGHUA XI1 1Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001; 2Department of Obstetrics and Gynecology, Affiliated Haian People's Hospital of Nantong University, Nantong, Jiangsu 226600; 3Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Care Hospital of Nantong University, Nantong, Jiangsu 226018; 4Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, P.R. China Received June 28, 2017; Accepted October 9, 2017 DOI: 10.3892/mmr.2018.8528 Abstract. Eph receptor‑A1 (EphA1) was the first member of 100%) and ovarian benign serous cystadenomas (12/12, 100%) the erythropoietin producing hepatocellular carcinoma (Eph) as well as in some borderline serous tumors (9/15, 60%) and family of receptor tyrosine kinases. Although the roles of ovarian serous carcinomas (33/76, 43.42%). EphA1 expression EphA1 in the tumorigenesis of various human cancers have was associated with grade of ovarian serous carcinomas, with been investigated, few studies have focused on ovarian carci- loss of EphA1 more often observed in high‑grade tumors noma. The present study aimed to explore the profile of EphA1 (P=0.016) and high Ki67 index tumors (P=0.007). These data expression in ovarian carcinomas, to analyzed the association suggest that EphA1 might be a useful marker for distinguishing between EphA1 expression and clinicopathologic parameters, low grade from high‑grade ovarian serous carcinoma. -
Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM 014911.2 KIAA1048, Dkfzp686k16132 AP2 Associated Kinase 1
Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM_014911.2 KIAA1048, DKFZp686K16132 AP2 associated kinase 1 (AAK1) AATK NM_001080395.2 AATYK, AATYK1, KIAA0641, LMR1, LMTK1, p35BP apoptosis-associated tyrosine kinase (AATK) ABL1 NM_007313.2 ABL, JTK7, c-ABL, p150 v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1) ABL2 NM_007314.3 ABLL, ARG v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2) ACVR1 NM_001105.2 ACVRLK2, SKR1, ALK2, ACVR1A activin A receptor ACVR1B NM_004302.3 ACVRLK4, ALK4, SKR2, ActRIB activin A receptor, type IB (ACVR1B) ACVR1C NM_145259.2 ACVRLK7, ALK7 activin A receptor, type IC (ACVR1C) ACVR2A NM_001616.3 ACVR2, ACTRII activin A receptor ACVR2B NM_001106.2 ActR-IIB activin A receptor ACVRL1 NM_000020.1 ACVRLK1, ORW2, HHT2, ALK1, HHT activin A receptor type II-like 1 (ACVRL1) ADCK1 NM_020421.2 FLJ39600 aarF domain containing kinase 1 (ADCK1) ADCK2 NM_052853.3 MGC20727 aarF domain containing kinase 2 (ADCK2) ADCK3 NM_020247.3 CABC1, COQ8, SCAR9 chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1) ADCK4 NM_024876.3 aarF domain containing kinase 4 (ADCK4) ADCK5 NM_174922.3 FLJ35454 aarF domain containing kinase 5 (ADCK5) ADRBK1 NM_001619.2 GRK2, BARK1 adrenergic, beta, receptor kinase 1 (ADRBK1) ADRBK2 NM_005160.2 GRK3, BARK2 adrenergic, beta, receptor kinase 2 (ADRBK2) AKT1 NM_001014431.1 RAC, PKB, PRKBA, AKT v-akt murine thymoma viral oncogene homolog 1 (AKT1) AKT2 NM_001626.2 v-akt murine thymoma viral oncogene homolog 2 (AKT2) AKT3 NM_181690.1