Viruses 2014, 6, 752-781; doi:10.3390/v6020752 OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Review Experimental Infections of Wild Birds with West Nile Virus Elisa Pérez-Ramírez *, Francisco Llorente and Miguel Ángel Jiménez-Clavero Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Valdeolmos (Madrid), 28130, Spain; E-Mails:
[email protected] (F.L.);
[email protected] (M.A.J.-C.) * Author to whom correspondence should be addressed; E-Mail:
[email protected]; Tel.: +34-91-6202300; Fax: +34-91-6202247. Received: 2 December 2013; in revised form: 4 February 2014 / Accepted: 4 February 2014 / Published: 13 February 2014 Abstract: Avian models of West Nile virus (WNV) disease have become pivotal in the study of infection pathogenesis and transmission, despite the intrinsic constraints that represents this type of experimental research that needs to be conducted in biosecurity level 3 (BSL3) facilities. This review summarizes the main achievements of WNV experimental research carried out in wild birds, highlighting advantages and limitations of this model. Viral and host factors that determine the infection outcome are analyzed in detail, as well as recent discoveries about avian immunity, viral transmission, and persistence achieved through experimental research. Studies of laboratory infections in the natural host will help to understand variations in susceptibility and reservoir competence among bird species, as well as in the epidemiological patterns found in different affected areas. Keywords: West Nile virus; wild birds; experimental infection; pathogenesis; transmission; immunity; host competence 1. Introduction West Nile virus (WNV, Flaviviridae, Flavivirus) is an emerging zoonotic arbovirus (arthropod- borne virus) widely distributed throughout the world and with considerable impact both on public health and on animal health [1].